RLG00000035323

WD repeat-containing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0007846
Physical Location & Seq
Reverse (-)
64968552 .. 64972002
3451 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035323

Sequence Viewer

Length: 1851 bp
ATGTACATGAACAACGGTTACAGCAGCGCCAGCACCAAAAACGGCGTCAATCTAACGTCGTCGGCGGCGGCAGCGACGACATCGGGCGCAACGACGTCGTCGTCCGGTGCGCAAACCCAGTCCCTGATAAAGGCGTATTTCAAGACACCGGAGGGGCGGTACAAGCTGCACTCGGAGAAGACGCGGCCGGCGAATCTGCTGCCCTATTCGTACGCCAAGACAATCTCGCAGGTCACGCTAGCCCAGCTGAAGGAGAGGCCCAACCAGGCGGCGCCGGGGCCGGTCCCCGGGAACATCGCCAGCGGGAGTGTGCGCCACGCGGCGGCGAGGCTGTTGGGGGTTGGGGCTGGAAATGGGAGCAAGGCGCTTAGTTATTTGGGCGGTGGGAGTAGTAAGACGAGTAATGGAAGTAGTAGGAGTGCTCATTTTGGAGGACTAGGGTCCAATGGGTATGGGGCCCATTCGTCGCCGACTTCGAGTCACGGCGGCAAAGGGTCGTTCTTGGTGTTCAATGTTGGAGATTCTATCTATATTTGCGATCTCAATTCGCCAGATAAGGATCCTATTAAAGCGTTGCATTTTAGTAATTCAAATCCGGTGTGTCACGCGTTTGATTCGGAAGCCAAGGATGGGCATGACTTGCTTATTGGATTGAGCTCTGGAGATATTTATTCAGCCTCTTTGAGACAGCAATTGCAAGATTCTGGGAAGAAGCTTGTTGGTGCCCTGCATTATAACAAAGAGGGTTCTGTTAATACCAGTCGCTGTACCAGTATTGCATGGGTTCCCAAAGGCAATGGCACTTTTATTGCTGCTCATGCTGATGGAAACTTGTATGAGTATGAGAAGGGCAAGGATAGTACCGGTGATACTTCTTTCCCAGTTATAAAGGATCCTGCTCAGTTTTCAGTGTATCATGCAAGATCCAGTAAGAGTAATCCAATTTCCAGATGGCATATTTGTGAAGGTTCAATAAACAGCATTGTTTACTCTCCGGATGGGACGTATTTGGCTACAGTTGGTAGAGATGGGTACTTGCGAGTATTTGACTACTCAAAAGAACAACTGATAGGTGGCGGCAAGAGTTATTATGGTTCTCTTCTATGTTGTACTTGGAGTCCGGATGGAAAATATGTTTTGACTGGAGGGGAAGATGATCTTGTTACAGTATGGAGCATGGAAGATAGGAAGGTTGTAGCTTGGGGTGAAGGACATAACTCATGGGTTAGTGGTGTGGCTTTTGACTCGCATTGGTCATCACCAACTTCGGATGGCACAGAAGAAAATCTTGTTTATCGCTTTGGCTCTGTTGGTCAGGACACAAAACTACTCCTGTGGGAACTCTCCATGGATGAGATTGTAGTGCCGCTTCGCCGGTGTTCAGTTGGTGGCTCTCCAACATTCAGCACGGGTAGCCAGTCATCCCATTGGGACAGCAGTTACCCGGTTGGCACTCTCCAACCTGCTCCAAGCATGCGGGATGTTCCCAAGCTCTCCCCACTAGTGGCTCACTGCGTTCACACCGAGCCCCTGTCTAGTTTAGCTTTTACCCAGGAAGCTATTCTCACTGCATCTTGGGAAGGGCATGTAAAAATTTGGATGAGACCAGGCGAACCCGAAAGTAAATCCACCAACTCTGAAGCTCTGCCAGGTGCTAGTTTGAGGGACCAACTACCACCGACAGGAAAAAGTTACTCAGAATCATTAAGGGGTTACCCATTGCCTGAAAGTAAATCAAACAACTCACAAGCTCTTCTAGGTGCTAGTTCAAAGGAGCAACAGCCATCGACGGGCAAAGGTGGTGCCTTTAGTTATAAAGACTTCTCAAAAAGCAACCGATACCTTCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

617

Amino Acids

65.56

Weight (kDa)

8.22

Isoelectric Point (pI)

42.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WDR55 PF24796 302 - 394 8.4e-07 WDR55
Beta-prop_WDR3_1st PF25173 311 - 452 4.9e-10 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 315 - 414 1.1e-09 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 320 - 415 4.1e-12 THOC3 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 321 - 425 1.4e-06 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 323 - 419 1.3e-13 WDR5 beta-propeller domain
Beta-prop_EML_2 PF23414 330 - 416 7e-08 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 735, 887, 1815
AasI GACNNNNNNGTC 1 cut(s) 100
AatII GACGTC 1 cut(s) 98
Acc16I TGCGCA 1 cut(s) 111
Acc36I ACCTGC 2 cut(s) 220, 1471
AccB1I GGYRCC 3 cut(s) 271, 722, 1802
AccII CGCG 3 cut(s) 184, 320, 608
AccIII TCCGGA 2 cut(s) 994, 1120
AclWI GGATC 5 cut(s) 554, 567, 887, 900, 918
AcoI YGGCCR 1 cut(s) 185
AcsI RAATTY 1 cut(s) 1593
AcuI CTGAAG 2 cut(s) 269, 1659
AcyI GRCGYC 3 cut(s) 45, 95, 272
AfaI GTAC 7 cut(s) 5, 161, 212, 769, 862, 1034, 1111
AfiI CCNNNNNNNGG 8 cut(s) 130, 250, 287, 322, 630, 1504, 1682, 1790
AflIII ACRYGT 1 cut(s) 606
AgeI ACCGGT 1 cut(s) 863
AgsI TTSAA 5 cut(s) 142, 511, 591, 972, 1770
AhdI GACNNNNNGTC 1 cut(s) 477
AhlI ACTAGT 1 cut(s) 1501
AjnI CCWGG 4 cut(s) 264, 1551, 1606, 1648
Alw21I GWGCWC 2 cut(s) 424, 659
Alw26I GTCTC 2 cut(s) 679, 1597
AlwI GGATC 5 cut(s) 554, 567, 887, 900, 918
AlwNI CAGNNNCTG 2 cut(s) 124, 765
Ama87I CYCGRG 1 cut(s) 287
Aor13HI TCCGGA 2 cut(s) 994, 1120
AoxI GGCC 4 cut(s) 185, 257, 278, 456
ApaI GGGCCC 1 cut(s) 460
ApeKI GCWGC 5 cut(s) 24, 71, 166, 199, 812
ApoI RAATTY 1 cut(s) 1593
ArsI GACNNNNNNTTYG 2 cut(s) 30, 62
AsiGI ACCGGT 1 cut(s) 863
Asp700I GAANNNNTTC 1 cut(s) 1560
AspLEI GCGC 6 cut(s) 29, 89, 112, 274, 315, 367
AspS9I GGNCC 7 cut(s) 258, 278, 283, 441, 456, 457, 1666
AsuC2I CCSGG 4 cut(s) 276, 288, 289, 1445
AsuHPI GGTGA 3 cut(s) 878, 1217, 1251
AsuNHI GCTAGC 1 cut(s) 238
AvaI CYCGRG 1 cut(s) 287
AvaII GGWCC 3 cut(s) 283, 441, 1666
BaeGI GKGCMC 2 cut(s) 460, 727
BamHI GGATCC 2 cut(s) 559, 892
BanI GGYRCC 3 cut(s) 271, 722, 1802
BanII GRGCYC 3 cut(s) 460, 659, 1530
BarI GAAGNNNNNNTAC 2 cut(s) 1353, 1385
BbsI GAAGAC 1 cut(s) 185
Bbv12I GWGCWC 2 cut(s) 424, 659
BbvI GCAGC 5 cut(s) 36, 83, 153, 186, 799
BccI CCATC 8 cut(s) 623, 818, 945, 992, 1022, 1118, 1265, 1792
BceAI ACGGC 2 cut(s) 58, 499
BcgI CGANNNNNNTGC 4 cut(s) 78, 112, 181, 215
BciT130I CCWGG 4 cut(s) 266, 1553, 1608, 1650
BcnI CCSGG 4 cut(s) 276, 288, 289, 1445
BcoDI GTCTC 2 cut(s) 679, 1597
BcuI ACTAGT 1 cut(s) 1501
BfaI CTAG 7 cut(s) 239, 437, 1502, 1536, 1656, 1757, 1764
BfmI CTRYAG 1 cut(s) 1014
BfoI RGCGCY 3 cut(s) 30, 275, 368
BfuAI ACCTGC 2 cut(s) 220, 1471
Bme1390I CCNGG 8 cut(s) 266, 276, 288, 289, 1445, 1553, 1608, 1650
Bme18I GGWCC 3 cut(s) 283, 441, 1666
BmeRI GACNNNNNGTC 1 cut(s) 477
BmeT110I CYCGRG 1 cut(s) 287
BmgT120I GGNCC 7 cut(s) 258, 278, 283, 441, 456, 457, 1666
BmrFI CCNGG 8 cut(s) 266, 276, 288, 289, 1445, 1553, 1608, 1650
BmrI ACTGGG 2 cut(s) 112, 875
BmsI GCATC 1 cut(s) 1580
BmtI GCTAGC 1 cut(s) 242
BmuI ACTGGG 2 cut(s) 112, 875
BoxI GACNNNNGTC 1 cut(s) 439
BpiI GAAGAC 1 cut(s) 185
BpmI CTGGAG 2 cut(s) 681, 1164
BpuMI CCSGG 4 cut(s) 276, 288, 289, 1445
BsaBI GATNNNNATC 1 cut(s) 558
BsaHI GRCGYC 3 cut(s) 45, 95, 272
BsaI GGTCTC 1 cut(s) 1597
BsaJI CCNNGG 6 cut(s) 275, 286, 287, 624, 1347, 1551
BsaWI WCCGGW 6 cut(s) 104, 148, 595, 863, 994, 1120
BsaXI ACNNNNNCTCC 2 cut(s) 143, 173
Bsc4I CCNNNNNNNGG 8 cut(s) 130, 250, 287, 322, 630, 1504, 1682, 1790
Bse118I RCCGGY 4 cut(s) 187, 280, 863, 1374
Bse1I ACTGG 7 cut(s) 118, 759, 771, 881, 927, 1147, 1417
Bse3DI GCAATG 2 cut(s) 802, 1718
Bse8I GATNNNNATC 1 cut(s) 558
BseAI TCCGGA 2 cut(s) 994, 1120
BseBI CCWGG 4 cut(s) 266, 1553, 1608, 1650
BseDI CCNNGG 6 cut(s) 275, 286, 287, 624, 1347, 1551
BseGI GGATG 8 cut(s) 634, 1003, 1129, 1276, 1357, 1421, 1486, 1605
BseJI GATNNNNATC 1 cut(s) 558
BseLI CCNNNNNNNGG 8 cut(s) 130, 250, 287, 322, 630, 1504, 1682, 1790
BseMI GCAATG 2 cut(s) 802, 1718
BseMII CTCAG 2 cut(s) 914, 1710
BseNI ACTGG 7 cut(s) 118, 759, 771, 881, 927, 1147, 1417
BseSI GKGCMC 2 cut(s) 460, 727
BseX3I CGGCCG 1 cut(s) 185
BseXI GCAGC 5 cut(s) 36, 83, 153, 186, 799
BseYI CCCAGC 1 cut(s) 243
BsgI GTGCAG 1 cut(s) 152
Bsh1236I CGCG 3 cut(s) 184, 320, 608
Bsh1285I CGRYCG 1 cut(s) 188
BshFI GGCC 4 cut(s) 187, 259, 280, 458
BshNI GGYRCC 3 cut(s) 271, 722, 1802
BshTI ACCGGT 1 cut(s) 863
BsiEI CGRYCG 1 cut(s) 188
BsiHKAI GWGCWC 2 cut(s) 424, 659
BsiHKCI CYCGRG 1 cut(s) 287
BsiWI CGTACG 1 cut(s) 210
BslFI GGGAC 5 cut(s) 106, 269, 1015, 1445, 1679
BslI CCNNNNNNNGG 8 cut(s) 130, 250, 287, 322, 630, 1504, 1682, 1790
BsmAI GTCTC 2 cut(s) 679, 1597
BsmFI GGGAC 5 cut(s) 106, 269, 1015, 1445, 1679
BsnI GGCC 4 cut(s) 187, 259, 280, 458
Bso31I GGTCTC 1 cut(s) 1597
BsoBI CYCGRG 1 cut(s) 287
Bsp120I GGGCCC 1 cut(s) 456
Bsp1286I GDGCHC 5 cut(s) 424, 460, 659, 727, 1530
Bsp13I TCCGGA 2 cut(s) 994, 1120
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 5 cut(s) 538, 559, 892, 923, 1156
Bsp19I CCATGG 1 cut(s) 1347
BspANI GGCC 4 cut(s) 187, 259, 280, 458
BspCNI CTCAG 2 cut(s) 913, 1709
BspEI TCCGGA 2 cut(s) 994, 1120
BspFNI CGCG 3 cut(s) 184, 320, 608
BspMI ACCTGC 2 cut(s) 220, 1471
BspOI GCTAGC 1 cut(s) 242
BspPI GGATC 5 cut(s) 554, 567, 887, 900, 918
BspQI GCTCTTC 1 cut(s) 1758
BspT107I GGYRCC 3 cut(s) 271, 722, 1802
BspTNI GGTCTC 1 cut(s) 1597
BsrDI GCAATG 2 cut(s) 802, 1718
BsrFI RCCGGY 4 cut(s) 187, 280, 863, 1374
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 7 cut(s) 118, 759, 771, 881, 927, 1147, 1417
BssAI RCCGGY 4 cut(s) 187, 280, 863, 1374
BssECI CCNNGG 6 cut(s) 275, 286, 287, 624, 1347, 1551
BssMI GATC 5 cut(s) 538, 559, 892, 923, 1156
BssNI GRCGYC 3 cut(s) 45, 95, 272
BssT1I CCWWGG 2 cut(s) 624, 1347
Bst2UI CCWGG 4 cut(s) 266, 1553, 1608, 1650
Bst4CI ACNGT 3 cut(s) 17, 1018, 1168
Bst6I CTCTTC 2 cut(s) 1104, 1758
BstACI GRCGYC 3 cut(s) 45, 95, 272
BstAPI GCANNNNNTGC 1 cut(s) 640
BstAUI TGTACA 1 cut(s) 3
BstC8I GCNNGC 5 cut(s) 31, 189, 240, 301, 1475
BstDEI CTNAG 3 cut(s) 368, 900, 1696
BstDSI CCRYGG 1 cut(s) 1347
BstEII GGTNACC 1 cut(s) 1712
BstENI CCTNNNNNAGG 1 cut(s) 128
BstF5I GGATG 8 cut(s) 634, 1003, 1129, 1276, 1357, 1421, 1486, 1605
BstFNI CGCG 3 cut(s) 184, 320, 608
BstH2I RGCGCY 3 cut(s) 30, 275, 368
BstHHI GCGC 6 cut(s) 29, 89, 112, 274, 315, 367
BstKTI GATC 5 cut(s) 541, 562, 895, 926, 1159
BstMAI GTCTC 2 cut(s) 679, 1597
BstMBI GATC 5 cut(s) 538, 559, 892, 923, 1156
BstMCI CGRYCG 1 cut(s) 188
BstMWI GCNNNNNNNGC 8 cut(s) 30, 71, 163, 235, 244, 640, 818, 1413
BstNI CCWGG 4 cut(s) 266, 1553, 1608, 1650
BstNSI RCATGY 2 cut(s) 1477, 1589
BstPAI GACNNNNGTC 1 cut(s) 439
BstPI GGTNACC 1 cut(s) 1712
BstSCI CCNGG 8 cut(s) 264, 274, 286, 287, 1443, 1551, 1606, 1648
BstSFI CTRYAG 1 cut(s) 1014
BstSLI GKGCMC 2 cut(s) 460, 727
BstUI CGCG 3 cut(s) 184, 320, 608
BstV1I GCAGC 5 cut(s) 36, 83, 153, 186, 799
BstV2I GAAGAC 1 cut(s) 185
BstX2I RGATCY 3 cut(s) 559, 892, 923
BstYI RGATCY 3 cut(s) 559, 892, 923
BstZI CGGCCG 1 cut(s) 185
BsuRI GGCC 4 cut(s) 187, 259, 280, 458
BtgI CCRYGG 1 cut(s) 1347
BtgZI GCGATG 1 cut(s) 280
BtsCI GGATG 8 cut(s) 634, 1003, 1129, 1276, 1357, 1421, 1486, 1605
BtsI GCAGTG 2 cut(s) 1510, 1566
BtsIMutI CAGTG 3 cut(s) 915, 1510, 1566
BveI ACCTGC 2 cut(s) 220, 1471
Cac8I GCNNGC 5 cut(s) 31, 189, 240, 301, 1475
CaiI CAGNNNCTG 2 cut(s) 124, 765
CfoI GCGC 6 cut(s) 29, 89, 112, 274, 315, 367
Cfr10I RCCGGY 4 cut(s) 187, 280, 863, 1374
Cfr13I GGNCC 7 cut(s) 258, 278, 283, 441, 456, 457, 1666
Cfr9I CCCGGG 1 cut(s) 287
CseI GACGC 2 cut(s) 34, 190
Csp6I GTAC 7 cut(s) 4, 160, 211, 768, 861, 1033, 1110
CspAI ACCGGT 1 cut(s) 863
CviQI GTAC 7 cut(s) 4, 160, 211, 768, 861, 1033, 1110
DdeI CTNAG 3 cut(s) 368, 900, 1696
DinI GGCGCC 1 cut(s) 273
DpnI GATC 5 cut(s) 540, 561, 894, 925, 1158
DpnII GATC 5 cut(s) 538, 559, 892, 923, 1156
DrdI GACNNNNNNGTC 1 cut(s) 100
DriI GACNNNNNGTC 1 cut(s) 477
DseDI GACNNNNNNGTC 1 cut(s) 100
EaeI YGGCCR 1 cut(s) 185
EagI CGGCCG 1 cut(s) 185
Eam1104I CTCTTC 2 cut(s) 1104, 1758
Eam1105I GACNNNNNGTC 1 cut(s) 477
EarI CTCTTC 2 cut(s) 1104, 1758
Ecl136II GAGCTC 1 cut(s) 657
EclXI CGGCCG 1 cut(s) 185
Eco130I CCWWGG 2 cut(s) 624, 1347
Eco24I GRGCYC 3 cut(s) 460, 659, 1530
Eco31I GGTCTC 1 cut(s) 1597
Eco47I GGWCC 3 cut(s) 283, 441, 1666
Eco52I CGGCCG 1 cut(s) 185
Eco53kI GAGCTC 1 cut(s) 657
Eco57I CTGAAG 2 cut(s) 269, 1659
Eco88I CYCGRG 1 cut(s) 287
Eco91I GGTNACC 1 cut(s) 1712
EcoICRI GAGCTC 1 cut(s) 657
EcoNI CCTNNNNNAGG 1 cut(s) 128
EcoO109I RGGNCCY 1 cut(s) 456
EcoO65I GGTNACC 1 cut(s) 1712
EcoRII CCWGG 4 cut(s) 264, 1551, 1606, 1648
EcoT14I CCWWGG 2 cut(s) 624, 1347
EcoT38I GRGCYC 3 cut(s) 460, 659, 1530
EgeI GGCGCC 1 cut(s) 273
EheI GGCGCC 1 cut(s) 273
ErhI CCWWGG 2 cut(s) 624, 1347
FalI AAGNNNNNCTT 4 cut(s) 1143, 1175, 1272, 1304
FaqI GGGAC 5 cut(s) 106, 269, 1015, 1445, 1679
FauI CCCGC 2 cut(s) 296, 1470
FokI GGATG 8 cut(s) 641, 1010, 1136, 1283, 1364, 1408, 1493, 1612
FriOI GRGCYC 3 cut(s) 460, 659, 1530
FspBI CTAG 7 cut(s) 239, 437, 1502, 1536, 1656, 1757, 1764
FspI TGCGCA 1 cut(s) 111
GlaI GCGC 6 cut(s) 28, 88, 111, 273, 314, 366
GsaI CCCAGC 1 cut(s) 247
GsuI CTGGAG 2 cut(s) 681, 1164
HaeII RGCGCY 3 cut(s) 30, 275, 368
HaeIII GGCC 4 cut(s) 187, 259, 280, 458
HgaI GACGC 2 cut(s) 34, 190
HhaI GCGC 6 cut(s) 29, 89, 112, 274, 315, 367
Hin1I GRCGYC 3 cut(s) 45, 95, 272
Hin6I GCGC 6 cut(s) 27, 87, 110, 272, 313, 365
HinP1I GCGC 6 cut(s) 27, 87, 110, 272, 313, 365
HindIII AAGCTT 1 cut(s) 713
HinfI GANTC 8 cut(s) 193, 478, 521, 614, 701, 1117, 1244, 1700
HphI GGTGA 3 cut(s) 878, 1217, 1251
Hpy166II GTNNAC 2 cut(s) 988, 1519
Hpy188I TCNGA 5 cut(s) 175, 619, 1270, 1639, 1699
Hpy188III TCNNGA 6 cut(s) 142, 660, 948, 995, 1121, 1316
Hpy8I GTNNAC 2 cut(s) 988, 1519
Hpy99I CGWCG 8 cut(s) 61, 64, 79, 97, 100, 103, 469, 1792
HpyAV CCTTC 6 cut(s) 244, 841, 959, 1183, 1202, 1574
HpyCH4III ACNGT 3 cut(s) 17, 1018, 1168
HpyCH4IV ACGT 3 cut(s) 56, 95, 1004
HpyCH4V TGCA 7 cut(s) 169, 577, 697, 730, 779, 920, 1571
HpyF10VI GCNNNNNNNGC 8 cut(s) 30, 71, 163, 235, 244, 640, 818, 1413
HpyF3I CTNAG 3 cut(s) 368, 900, 1696
HpySE526I ACGT 3 cut(s) 56, 95, 1004
Hsp92I GRCGYC 3 cut(s) 45, 95, 272
HspAI GCGC 6 cut(s) 27, 87, 110, 272, 313, 365
KasI GGCGCC 1 cut(s) 271
Kpn2I TCCGGA 2 cut(s) 994, 1120
KroI GCCGGC 1 cut(s) 187
KroNI GCCGGC 1 cut(s) 189
Kzo9I GATC 5 cut(s) 538, 559, 892, 923, 1156
LguI GCTCTTC 1 cut(s) 1758
LmnI GCTCC 4 cut(s) 357, 1173, 1471, 1774
Lsp1109I GCAGC 5 cut(s) 36, 83, 153, 186, 799
LweI GCATC 1 cut(s) 1580
MaeI CTAG 7 cut(s) 239, 437, 1502, 1536, 1656, 1757, 1764
MaeII ACGT 3 cut(s) 56, 95, 1004
MaeIII GTNAC 8 cut(s) 17, 232, 479, 602, 1162, 1439, 1691, 1712
MalI GATC 5 cut(s) 540, 561, 894, 925, 1158
MboI GATC 5 cut(s) 538, 559, 892, 923, 1156
MboII GAAGA 7 cut(s) 190, 721, 1091, 1163, 1193, 1292, 1745
MfeI CAATTG 1 cut(s) 692
MflI RGATCY 3 cut(s) 559, 892, 923
MhlI GDGCHC 5 cut(s) 424, 460, 659, 727, 1530
MluCI AATT 5 cut(s) 544, 586, 692, 942, 1593
MluI ACGCGT 1 cut(s) 606
Mly113I GGCGCC 1 cut(s) 272
MlyI GAGTC 3 cut(s) 487, 1126, 1238
MmeI TCCRAC 3 cut(s) 496, 1421, 1483
MnlI CCTC 8 cut(s) 145, 249, 321, 425, 688, 736, 1139, 1656
MroI TCCGGA 2 cut(s) 994, 1120
MroNI GCCGGC 1 cut(s) 187
MroXI GAANNNNTTC 1 cut(s) 1560
MseI TTAA 3 cut(s) 567, 753, 1706
MslI CAYNNNNRTG 2 cut(s) 822, 960
MspA1I CMGCKG 2 cut(s) 247, 303
MspR9I CCNGG 8 cut(s) 266, 276, 288, 289, 1445, 1553, 1608, 1650
MunI CAATTG 1 cut(s) 692
MvaI CCWGG 4 cut(s) 266, 1553, 1608, 1650
MvnI CGCG 3 cut(s) 184, 320, 608
MwoI GCNNNNNNNGC 8 cut(s) 30, 71, 163, 235, 244, 640, 818, 1413
NaeI GCCGGC 1 cut(s) 189
NarI GGCGCC 1 cut(s) 272
NciI CCSGG 4 cut(s) 276, 288, 289, 1445
NcoI CCATGG 1 cut(s) 1347
NdeII GATC 5 cut(s) 538, 559, 892, 923, 1156
NgoMIV GCCGGC 1 cut(s) 187
NheI GCTAGC 1 cut(s) 238
NmuCI GTSAC 3 cut(s) 232, 479, 602
NsbI TGCGCA 1 cut(s) 111
NspI RCATGY 2 cut(s) 1477, 1589
PaeI GCATGC 1 cut(s) 1477
PciSI GCTCTTC 1 cut(s) 1758
PcsI WCGNNNNNNNCGW 4 cut(s) 89, 98, 188, 473
PdiI GCCGGC 1 cut(s) 189
PdmI GAANNNNTTC 1 cut(s) 1560
PfeI GAWTC 5 cut(s) 193, 521, 614, 701, 1700
Pfl23II CGTACG 1 cut(s) 210
PflFI GACNNNGTC 1 cut(s) 97
PinAI ACCGGT 1 cut(s) 863
PleI GAGTC 3 cut(s) 486, 1125, 1238
PluTI GGCGCC 1 cut(s) 275
PpsI GAGTC 3 cut(s) 486, 1125, 1238
PshAI GACNNNNGTC 1 cut(s) 439
PsiI TTATAA 3 cut(s) 735, 887, 1815
Psp124BI GAGCTC 1 cut(s) 659
Psp6I CCWGG 4 cut(s) 264, 1551, 1606, 1648
PspEI GGTNACC 1 cut(s) 1712
PspFI CCCAGC 1 cut(s) 243
PspGI CCWGG 4 cut(s) 264, 1551, 1606, 1648
PspLI CGTACG 1 cut(s) 210
PspOMI GGGCCC 1 cut(s) 456
PspPI GGNCC 7 cut(s) 258, 278, 283, 441, 456, 457, 1666
PsrI GAACNNNNNNTAC 1 cut(s) 34
PstNI CAGNNNCTG 2 cut(s) 124, 765
PsuI RGATCY 3 cut(s) 559, 892, 923
PsyI GACNNNGTC 1 cut(s) 97
PvuII CAGCTG 1 cut(s) 247
RsaI GTAC 7 cut(s) 5, 161, 212, 769, 862, 1034, 1111
RsaNI GTAC 7 cut(s) 4, 160, 211, 768, 861, 1033, 1110
RseI CAYNNNNRTG 2 cut(s) 822, 960
SacI GAGCTC 1 cut(s) 659
SapI GCTCTTC 1 cut(s) 1758
SaqAI TTAA 3 cut(s) 567, 753, 1706
Sau3AI GATC 5 cut(s) 538, 559, 892, 923, 1156
Sau96I GGNCC 7 cut(s) 258, 278, 283, 441, 456, 457, 1666
SchI GAGTC 3 cut(s) 487, 1126, 1238
ScrFI CCNGG 8 cut(s) 266, 276, 288, 289, 1445, 1553, 1608, 1650
SduI GDGCHC 5 cut(s) 424, 460, 659, 727, 1530
SfaNI GCATC 1 cut(s) 1580
SfcI CTRYAG 1 cut(s) 1014
SfoI GGCGCC 1 cut(s) 273
SgrAI CRCCGGYG 1 cut(s) 1374
SinI GGWCC 3 cut(s) 283, 441, 1666
SmaI CCCGGG 1 cut(s) 289
SmiMI CAYNNNNRTG 2 cut(s) 822, 960
SpeI ACTAGT 1 cut(s) 1501
SphI GCATGC 1 cut(s) 1477
Sse9I AATT 5 cut(s) 544, 586, 692, 942, 1593
SspDI GGCGCC 1 cut(s) 271
SspMI CTAG 7 cut(s) 239, 437, 1502, 1536, 1656, 1757, 1764
SstI GAGCTC 1 cut(s) 659
StyD4I CCNGG 8 cut(s) 264, 274, 286, 287, 1443, 1551, 1606, 1648
StyI CCWWGG 2 cut(s) 624, 1347
TaaI ACNGT 3 cut(s) 17, 1018, 1168
TaiI ACGT 3 cut(s) 59, 98, 1007
TaqI TCGA 2 cut(s) 476, 1787
TasI AATT 5 cut(s) 544, 586, 692, 942, 1593
TatI WGTACW 2 cut(s) 3, 1109
TauI GCSGC 9 cut(s) 68, 71, 187, 272, 323, 326, 489, 1080, 1369
TfiI GAWTC 5 cut(s) 193, 521, 614, 701, 1700
Tru1I TTAA 3 cut(s) 567, 753, 1706
Tru9I TTAA 3 cut(s) 567, 753, 1706
TscAI CASTG 3 cut(s) 915, 1517, 1573
TseFI GTSAC 3 cut(s) 232, 479, 602
TseI GCWGC 5 cut(s) 24, 71, 166, 199, 812
Tsp45I GTSAC 3 cut(s) 232, 479, 602
TspDTI ATGAA 1 cut(s) 23
TspMI CCCGGG 1 cut(s) 287
TspRI CASTG 3 cut(s) 915, 1517, 1573
Tth111I GACNNNGTC 1 cut(s) 97
VpaK11BI GGWCC 3 cut(s) 283, 441, 1666
XagI CCTNNNNNAGG 1 cut(s) 128
XapI RAATTY 1 cut(s) 1593
XceI RCATGY 2 cut(s) 1477, 1589
XcmI CCANNNNNNNNNTGG 1 cut(s) 948
XmaI CCCGGG 1 cut(s) 287
XmnI GAANNNNTTC 1 cut(s) 1560
XspI CTAG 7 cut(s) 239, 437, 1502, 1536, 1656, 1757, 1764
ZraI GACGTC 1 cut(s) 96
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.