Rh5DG417900

WD repeat-containing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0007846
Physical Location & Seq
Reverse (-)
66392370 .. 66396262
3893 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG417900.1

Sequence Viewer

Length: 1848 bp
ATGTACATGAACAACGGTTACAGCAGCGCCAGCACCAAAAACGGCGTCAATCTAACGTCGTCGTCGTCGGCGACGACGTCGGGCGCAACGACGTCGGCGTCCGGTGGGCAAACCCAGTCCCTGATAAAGGCGTATTTCAAGACCCCGGAGGGGCGGTACAAGCTGCACTCGGAGAAGACGCGGCCGGCGAATCTGCTGCCCTATTCGTACGCCAAGACAATCTCGCAGGTCACGCTAGCCCAGCTGAAGGAGAGGCCCAACCAGGCGGCGCCGGGGCCGGTCCCCGGGAACATCGCCAGCGGGAGTGTGCGCCACGCGGCGGCGAGGCTGTTGGGGGTTGGGGCTGGAAATGGGAGCAAGGCGCTTAGTTATTTGGGCGGTGGGAGCAGTAAGACGAGTAATGGAAGTAGTAGGAGTACTCATTTTGGAGGACTAGGGTCCAATGGGTATGGGGCCCATTCGTCGCCGACTTCGAGTCACGGCGGTAAAGGGTCGTTCTTGGTGTTCAATGTTGGAGATACTATCTATATTTGCGATCTCAATTCGCCAGATAAGGATCCTATTAAAGCGTTGCATTTTAGTAATTCGAATCCGGTGTGTCACGCGTTTGATTCGGAAGCCAAGGATGGGCATGACTTGCTTATTGGATTGAGCTCTGGAGATATTTATTCAGCCTCTTTGAGACAGCAATTGCAAGATTCTGGGAAGAAGCTTGTTGGTGCCCTGCATTATAACAAAGAGGGTTCTGTTAATACCAGTCGCTGTACCAGTATTGCATGGGTTCCCAAAGGCAATGGTACTTTTATTGCTGCTCATGCTGATGGAAACTTGTACGAATATGAGAAGGGCAAGGATATTACCGGTGATACTTCTTTCCCAGTTATAAAGGATCCTGCTCAGTTTTCAGTGTATCATGCAAGATCCAGTAAGAGTAATCCAATTTCCAGATGGCATATTTGTGAAGGTTCAATAAACAGCATTGTTTACTCTCCGGATGGGACATATTTGGCTACAGTTGGTAGAGATGGGTACTTGCGAGTATTTGACTACTCAAAAGAACAACTGATAGGTGGCGGCAAGAGTTATTATGGTTCTCTTCTATGTTGTACTTGGAGTCCGGATGGAAAATATGTTTTGACTGGAGGGGAAGATGATCTTGTTACAGTATGGAGCATGGAAGATAGGAAGGTTGTAGCTTGGGGTGAAGGACATAACTCATGGGTTAGTGGTTTGGCTTTTGACTCGCATTGGTCATCACCAACTTCGGATGGCACAGAAGAAAATCTTGTTTATCGCTTTGGCTCTGTTGGTCAGGACACAAAACTACTCCTGTGGGAACTCTCCATGGATGAGATTGTAGTGCCGCTTCGCCGGTGTTCAGTTGGTGGCTCTCCAACATTCAGCACTGGTAGCCAGTCATCCCATTGGGACAGCAGTTACCCGGTTGGCACTCTCCAACCTGCTCCAAGCATGCGGGATGTTCCCAAGCTCTCCCCACTAGTGGCTCACTGCGTTCACACCGAGCCCCTGTCTAGTTTAGCTTTTACCCAGGAAGCTATTCTCACTGCATCTTGGGAAGGGCATGTAAAAATTTGGATGAGACCAGGTGAACCCGAAAGTAAATCCACCAACTCAGAAGCTCTGCCAGGTGCTAGTTTGAGGGACCAACTACCACCGACAGGAAAAAGTTACTCAGAATCATTGAGGGGTTACCCATTGCCTGAAAGTAAATCAAACAACTCACAAGCTCTTCTAGGTGCTAGTTCAAAGGAGCAACAGCCATCGACGGGCAAAGGTGGTGCCTTTAGTTATAAAGACTTCTCAAAAAGCAACCGATACCTTCCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

615

Amino Acids

65.58

Weight (kDa)

8.22

Isoelectric Point (pI)

42.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WDR55 PF24796 303 - 393 7.3e-07 WDR55
Beta-prop_WDR3_1st PF25173 310 - 451 8e-10 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 314 - 413 6.2e-10 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 319 - 414 6.7e-12 THOC3 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 320 - 424 5e-06 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 321 - 420 3.2e-13 WDR5 beta-propeller domain
Beta-prop_EML_2 PF23414 329 - 415 9e-08 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 732, 884, 1812
AasI GACNNNNNNGTC 1 cut(s) 97
AatII GACGTC 2 cut(s) 80, 95
Acc36I ACCTGC 2 cut(s) 217, 1468
AccB1I GGYRCC 3 cut(s) 268, 719, 1799
AccII CGCG 3 cut(s) 181, 317, 605
AccIII TCCGGA 2 cut(s) 991, 1117
AclWI GGATC 5 cut(s) 551, 564, 884, 897, 915
AcoI YGGCCR 1 cut(s) 182
AcsI RAATTY 1 cut(s) 1590
AcuI CTGAAG 1 cut(s) 266
AcyI GRCGYC 5 cut(s) 45, 77, 92, 98, 269
AfaI GTAC 9 cut(s) 5, 158, 209, 418, 766, 799, 833, 1031, 1108
AfiI CCNNNNNNNGG 9 cut(s) 127, 150, 247, 284, 319, 627, 1501, 1679, 1787
AflIII ACRYGT 1 cut(s) 603
AgeI ACCGGT 1 cut(s) 860
AgsI TTSAA 4 cut(s) 139, 508, 969, 1767
AhdI GACNNNNNGTC 1 cut(s) 474
AhlI ACTAGT 1 cut(s) 1498
AjnI CCWGG 4 cut(s) 261, 1548, 1603, 1645
Alw21I GWGCWC 1 cut(s) 656
Alw26I GTCTC 2 cut(s) 676, 1594
AlwI GGATC 5 cut(s) 551, 564, 884, 897, 915
AlwNI CAGNNNCTG 2 cut(s) 121, 762
Ama87I CYCGRG 1 cut(s) 284
Aor13HI TCCGGA 2 cut(s) 991, 1117
AoxI GGCC 4 cut(s) 182, 254, 275, 453
ApaI GGGCCC 1 cut(s) 457
ApeKI GCWGC 4 cut(s) 24, 163, 196, 809
ApoI RAATTY 1 cut(s) 1590
ArsI GACNNNNNNTTYG 2 cut(s) 30, 62
AsiGI ACCGGT 1 cut(s) 860
Asp700I GAANNNNTTC 1 cut(s) 1557
AspLEI GCGC 5 cut(s) 29, 86, 271, 312, 364
AspS9I GGNCC 7 cut(s) 255, 275, 280, 438, 453, 454, 1663
AsuC2I CCSGG 5 cut(s) 146, 273, 285, 286, 1442
AsuHPI GGTGA 4 cut(s) 875, 1214, 1248, 1619
AsuII TTCGAA 1 cut(s) 587
AsuNHI GCTAGC 1 cut(s) 235
AvaI CYCGRG 1 cut(s) 284
AvaII GGWCC 3 cut(s) 280, 438, 1663
BaeGI GKGCMC 2 cut(s) 457, 724
BamHI GGATCC 2 cut(s) 556, 889
BanI GGYRCC 3 cut(s) 268, 719, 1799
BanII GRGCYC 3 cut(s) 457, 656, 1527
BarI GAAGNNNNNNTAC 2 cut(s) 1350, 1382
BbsI GAAGAC 1 cut(s) 182
Bbv12I GWGCWC 1 cut(s) 656
BbvI GCAGC 4 cut(s) 36, 150, 183, 796
BccI CCATC 8 cut(s) 620, 815, 942, 989, 1019, 1115, 1262, 1789
BceAI ACGGC 2 cut(s) 58, 496
BcgI CGANNNNNNTGC 4 cut(s) 75, 109, 178, 212
BciT130I CCWGG 4 cut(s) 263, 1550, 1605, 1647
BcnI CCSGG 5 cut(s) 146, 273, 285, 286, 1442
BcoDI GTCTC 2 cut(s) 676, 1594
BcuI ACTAGT 1 cut(s) 1498
BfaI CTAG 7 cut(s) 236, 434, 1499, 1533, 1653, 1754, 1761
BfmI CTRYAG 1 cut(s) 1011
BfoI RGCGCY 3 cut(s) 30, 272, 365
BfuAI ACCTGC 2 cut(s) 217, 1468
BmcAI AGTACT 1 cut(s) 418
Bme1390I CCNGG 9 cut(s) 146, 263, 273, 285, 286, 1442, 1550, 1605, 1647
Bme18I GGWCC 3 cut(s) 280, 438, 1663
BmeRI GACNNNNNGTC 1 cut(s) 474
BmeT110I CYCGRG 1 cut(s) 284
BmgT120I GGNCC 7 cut(s) 255, 275, 280, 438, 453, 454, 1663
BmrFI CCNGG 9 cut(s) 146, 263, 273, 285, 286, 1442, 1550, 1605, 1647
BmrI ACTGGG 2 cut(s) 109, 872
BmsI GCATC 1 cut(s) 1577
BmtI GCTAGC 1 cut(s) 239
BmuI ACTGGG 2 cut(s) 109, 872
BoxI GACNNNNGTC 1 cut(s) 436
BpiI GAAGAC 1 cut(s) 182
BpmI CTGGAG 2 cut(s) 678, 1161
Bpu14I TTCGAA 1 cut(s) 587
BpuMI CCSGG 5 cut(s) 146, 273, 285, 286, 1442
BsaBI GATNNNNATC 1 cut(s) 555
BsaHI GRCGYC 5 cut(s) 45, 77, 92, 98, 269
BsaI GGTCTC 1 cut(s) 1594
BsaJI CCNNGG 7 cut(s) 144, 272, 283, 284, 621, 1344, 1548
BsaWI WCCGGW 5 cut(s) 101, 592, 860, 991, 1117
BsaXI ACNNNNNCTCC 2 cut(s) 140, 170
Bsc4I CCNNNNNNNGG 9 cut(s) 127, 150, 247, 284, 319, 627, 1501, 1679, 1787
Bse118I RCCGGY 4 cut(s) 184, 277, 860, 1371
Bse1I ACTGG 8 cut(s) 115, 756, 768, 878, 924, 1144, 1411, 1414
Bse3DI GCAATG 2 cut(s) 799, 1715
Bse8I GATNNNNATC 1 cut(s) 555
BseAI TCCGGA 2 cut(s) 991, 1117
BseBI CCWGG 4 cut(s) 263, 1550, 1605, 1647
BseDI CCNNGG 7 cut(s) 144, 272, 283, 284, 621, 1344, 1548
BseGI GGATG 8 cut(s) 631, 1000, 1126, 1273, 1354, 1418, 1483, 1602
BseJI GATNNNNATC 1 cut(s) 555
BseLI CCNNNNNNNGG 9 cut(s) 127, 150, 247, 284, 319, 627, 1501, 1679, 1787
BseMI GCAATG 2 cut(s) 799, 1715
BseMII CTCAG 3 cut(s) 911, 1647, 1707
BseNI ACTGG 8 cut(s) 115, 756, 768, 878, 924, 1144, 1411, 1414
BseSI GKGCMC 2 cut(s) 457, 724
BseX3I CGGCCG 1 cut(s) 182
BseXI GCAGC 4 cut(s) 36, 150, 183, 796
BseYI CCCAGC 1 cut(s) 240
BsgI GTGCAG 1 cut(s) 149
Bsh1236I CGCG 3 cut(s) 181, 317, 605
Bsh1285I CGRYCG 1 cut(s) 185
BshFI GGCC 4 cut(s) 184, 256, 277, 455
BshNI GGYRCC 3 cut(s) 268, 719, 1799
BshTI ACCGGT 1 cut(s) 860
BsiEI CGRYCG 1 cut(s) 185
BsiHKAI GWGCWC 1 cut(s) 656
BsiHKCI CYCGRG 1 cut(s) 284
BsiWI CGTACG 1 cut(s) 207
BslFI GGGAC 5 cut(s) 103, 266, 1012, 1442, 1676
BslI CCNNNNNNNGG 9 cut(s) 127, 150, 247, 284, 319, 627, 1501, 1679, 1787
BsmAI GTCTC 2 cut(s) 676, 1594
BsmFI GGGAC 5 cut(s) 103, 266, 1012, 1442, 1676
BsnI GGCC 4 cut(s) 184, 256, 277, 455
Bso31I GGTCTC 1 cut(s) 1594
BsoBI CYCGRG 1 cut(s) 284
Bsp119I TTCGAA 1 cut(s) 587
Bsp120I GGGCCC 1 cut(s) 453
Bsp1286I GDGCHC 4 cut(s) 457, 656, 724, 1527
Bsp13I TCCGGA 2 cut(s) 991, 1117
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 5 cut(s) 535, 556, 889, 920, 1153
Bsp19I CCATGG 1 cut(s) 1344
BspANI GGCC 4 cut(s) 184, 256, 277, 455
BspCNI CTCAG 3 cut(s) 910, 1646, 1706
BspEI TCCGGA 2 cut(s) 991, 1117
BspFNI CGCG 3 cut(s) 181, 317, 605
BspMI ACCTGC 2 cut(s) 217, 1468
BspOI GCTAGC 1 cut(s) 239
BspPI GGATC 5 cut(s) 551, 564, 884, 897, 915
BspQI GCTCTTC 1 cut(s) 1755
BspT104I TTCGAA 1 cut(s) 587
BspT107I GGYRCC 3 cut(s) 268, 719, 1799
BspTNI GGTCTC 1 cut(s) 1594
BsrDI GCAATG 2 cut(s) 799, 1715
BsrFI RCCGGY 4 cut(s) 184, 277, 860, 1371
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 8 cut(s) 115, 756, 768, 878, 924, 1144, 1411, 1414
BssAI RCCGGY 4 cut(s) 184, 277, 860, 1371
BssECI CCNNGG 7 cut(s) 144, 272, 283, 284, 621, 1344, 1548
BssMI GATC 5 cut(s) 535, 556, 889, 920, 1153
BssNI GRCGYC 5 cut(s) 45, 77, 92, 98, 269
BssT1I CCWWGG 2 cut(s) 621, 1344
Bst2UI CCWGG 4 cut(s) 263, 1550, 1605, 1647
Bst4CI ACNGT 3 cut(s) 17, 1015, 1165
Bst6I CTCTTC 2 cut(s) 1101, 1755
BstACI GRCGYC 5 cut(s) 45, 77, 92, 98, 269
BstAPI GCANNNNNTGC 1 cut(s) 637
BstAUI TGTACA 1 cut(s) 3
BstBI TTCGAA 1 cut(s) 587
BstC8I GCNNGC 5 cut(s) 31, 186, 237, 298, 1472
BstDEI CTNAG 4 cut(s) 365, 897, 1633, 1693
BstDSI CCRYGG 1 cut(s) 1344
BstEII GGTNACC 1 cut(s) 1709
BstENI CCTNNNNNAGG 1 cut(s) 125
BstF5I GGATG 8 cut(s) 631, 1000, 1126, 1273, 1354, 1418, 1483, 1602
BstFNI CGCG 3 cut(s) 181, 317, 605
BstH2I RGCGCY 3 cut(s) 30, 272, 365
BstHHI GCGC 5 cut(s) 29, 86, 271, 312, 364
BstKTI GATC 5 cut(s) 538, 559, 892, 923, 1156
BstMAI GTCTC 2 cut(s) 676, 1594
BstMBI GATC 5 cut(s) 535, 556, 889, 920, 1153
BstMCI CGRYCG 1 cut(s) 185
BstMWI GCNNNNNNNGC 8 cut(s) 30, 160, 232, 241, 384, 637, 815, 1410
BstNI CCWGG 4 cut(s) 263, 1550, 1605, 1647
BstNSI RCATGY 2 cut(s) 1474, 1586
BstPAI GACNNNNGTC 1 cut(s) 436
BstPI GGTNACC 1 cut(s) 1709
BstSCI CCNGG 9 cut(s) 144, 261, 271, 283, 284, 1440, 1548, 1603, 1645
BstSFI CTRYAG 1 cut(s) 1011
BstSLI GKGCMC 2 cut(s) 457, 724
BstUI CGCG 3 cut(s) 181, 317, 605
BstV1I GCAGC 4 cut(s) 36, 150, 183, 796
BstV2I GAAGAC 1 cut(s) 182
BstX2I RGATCY 3 cut(s) 556, 889, 920
BstYI RGATCY 3 cut(s) 556, 889, 920
BstZI CGGCCG 1 cut(s) 182
BsuRI GGCC 4 cut(s) 184, 256, 277, 455
BtgI CCRYGG 1 cut(s) 1344
BtgZI GCGATG 1 cut(s) 277
BtsCI GGATG 8 cut(s) 631, 1000, 1126, 1273, 1354, 1418, 1483, 1602
BtsI GCAGTG 2 cut(s) 1507, 1563
BtsIMutI CAGTG 4 cut(s) 912, 1404, 1507, 1563
BveI ACCTGC 2 cut(s) 217, 1468
Cac8I GCNNGC 5 cut(s) 31, 186, 237, 298, 1472
CaiI CAGNNNCTG 2 cut(s) 121, 762
CfoI GCGC 5 cut(s) 29, 86, 271, 312, 364
Cfr10I RCCGGY 4 cut(s) 184, 277, 860, 1371
Cfr13I GGNCC 7 cut(s) 255, 275, 280, 438, 453, 454, 1663
Cfr9I CCCGGG 1 cut(s) 284
CseI GACGC 3 cut(s) 34, 87, 187
CsiI ACCWGGT 1 cut(s) 1603
Csp6I GTAC 9 cut(s) 4, 157, 208, 417, 765, 798, 832, 1030, 1107
CspAI ACCGGT 1 cut(s) 860
CviQI GTAC 9 cut(s) 4, 157, 208, 417, 765, 798, 832, 1030, 1107
DdeI CTNAG 4 cut(s) 365, 897, 1633, 1693
DinI GGCGCC 1 cut(s) 270
DpnI GATC 5 cut(s) 537, 558, 891, 922, 1155
DpnII GATC 5 cut(s) 535, 556, 889, 920, 1153
DrdI GACNNNNNNGTC 1 cut(s) 97
DriI GACNNNNNGTC 1 cut(s) 474
DseDI GACNNNNNNGTC 1 cut(s) 97
EaeI YGGCCR 1 cut(s) 182
EagI CGGCCG 1 cut(s) 182
Eam1104I CTCTTC 2 cut(s) 1101, 1755
Eam1105I GACNNNNNGTC 1 cut(s) 474
EarI CTCTTC 2 cut(s) 1101, 1755
Ecl136II GAGCTC 1 cut(s) 654
EclXI CGGCCG 1 cut(s) 182
Eco130I CCWWGG 2 cut(s) 621, 1344
Eco24I GRGCYC 3 cut(s) 457, 656, 1527
Eco31I GGTCTC 1 cut(s) 1594
Eco47I GGWCC 3 cut(s) 280, 438, 1663
Eco52I CGGCCG 1 cut(s) 182
Eco53kI GAGCTC 1 cut(s) 654
Eco57I CTGAAG 1 cut(s) 266
Eco88I CYCGRG 1 cut(s) 284
Eco91I GGTNACC 1 cut(s) 1709
EcoICRI GAGCTC 1 cut(s) 654
EcoNI CCTNNNNNAGG 1 cut(s) 125
EcoO109I RGGNCCY 1 cut(s) 453
EcoO65I GGTNACC 1 cut(s) 1709
EcoRII CCWGG 4 cut(s) 261, 1548, 1603, 1645
EcoT14I CCWWGG 2 cut(s) 621, 1344
EcoT38I GRGCYC 3 cut(s) 457, 656, 1527
EgeI GGCGCC 1 cut(s) 270
EheI GGCGCC 1 cut(s) 270
ErhI CCWWGG 2 cut(s) 621, 1344
FalI AAGNNNNNCTT 4 cut(s) 1140, 1172, 1269, 1301
FaqI GGGAC 5 cut(s) 103, 266, 1012, 1442, 1676
FauI CCCGC 2 cut(s) 293, 1467
FokI GGATG 8 cut(s) 638, 1007, 1133, 1280, 1361, 1405, 1490, 1609
FriOI GRGCYC 3 cut(s) 457, 656, 1527
FspBI CTAG 7 cut(s) 236, 434, 1499, 1533, 1653, 1754, 1761
GlaI GCGC 5 cut(s) 28, 85, 270, 311, 363
GsaI CCCAGC 1 cut(s) 244
GsuI CTGGAG 2 cut(s) 678, 1161
HaeII RGCGCY 3 cut(s) 30, 272, 365
HaeIII GGCC 4 cut(s) 184, 256, 277, 455
HgaI GACGC 3 cut(s) 34, 87, 187
HhaI GCGC 5 cut(s) 29, 86, 271, 312, 364
Hin1I GRCGYC 5 cut(s) 45, 77, 92, 98, 269
Hin6I GCGC 5 cut(s) 27, 84, 269, 310, 362
HinP1I GCGC 5 cut(s) 27, 84, 269, 310, 362
HindIII AAGCTT 1 cut(s) 710
HinfI GANTC 8 cut(s) 190, 475, 589, 611, 698, 1114, 1241, 1697
HphI GGTGA 4 cut(s) 875, 1214, 1248, 1619
Hpy166II GTNNAC 3 cut(s) 985, 1516, 1610
Hpy188I TCNGA 5 cut(s) 172, 616, 1267, 1636, 1696
Hpy188III TCNNGA 6 cut(s) 139, 657, 945, 992, 1118, 1313
Hpy8I GTNNAC 3 cut(s) 985, 1516, 1610
HpyAV CCTTC 6 cut(s) 241, 838, 956, 1180, 1199, 1571
HpyCH4III ACNGT 3 cut(s) 17, 1015, 1165
HpyCH4IV ACGT 3 cut(s) 56, 77, 92
HpyCH4V TGCA 7 cut(s) 166, 574, 694, 727, 776, 917, 1568
HpyF10VI GCNNNNNNNGC 8 cut(s) 30, 160, 232, 241, 384, 637, 815, 1410
HpyF3I CTNAG 4 cut(s) 365, 897, 1633, 1693
HpySE526I ACGT 3 cut(s) 56, 77, 92
Hsp92I GRCGYC 5 cut(s) 45, 77, 92, 98, 269
HspAI GCGC 5 cut(s) 27, 84, 269, 310, 362
KasI GGCGCC 1 cut(s) 268
Kpn2I TCCGGA 2 cut(s) 991, 1117
KroI GCCGGC 1 cut(s) 184
KroNI GCCGGC 1 cut(s) 186
Kzo9I GATC 5 cut(s) 535, 556, 889, 920, 1153
LguI GCTCTTC 1 cut(s) 1755
LmnI GCTCC 5 cut(s) 354, 384, 1170, 1468, 1771
Lsp1109I GCAGC 4 cut(s) 36, 150, 183, 796
LweI GCATC 1 cut(s) 1577
MabI ACCWGGT 1 cut(s) 1603
MaeI CTAG 7 cut(s) 236, 434, 1499, 1533, 1653, 1754, 1761
MaeII ACGT 3 cut(s) 56, 77, 92
MaeIII GTNAC 8 cut(s) 17, 229, 476, 599, 1159, 1436, 1688, 1709
MalI GATC 5 cut(s) 537, 558, 891, 922, 1155
MboI GATC 5 cut(s) 535, 556, 889, 920, 1153
MboII GAAGA 7 cut(s) 187, 718, 1088, 1160, 1190, 1289, 1742
MfeI CAATTG 1 cut(s) 689
MflI RGATCY 3 cut(s) 556, 889, 920
MhlI GDGCHC 4 cut(s) 457, 656, 724, 1527
MluCI AATT 5 cut(s) 541, 583, 689, 939, 1590
MluI ACGCGT 1 cut(s) 603
Mly113I GGCGCC 1 cut(s) 269
MlyI GAGTC 3 cut(s) 484, 1123, 1235
MmeI TCCRAC 3 cut(s) 493, 1418, 1480
MnlI CCTC 9 cut(s) 142, 246, 318, 422, 685, 733, 1136, 1653, 1698
MroI TCCGGA 2 cut(s) 991, 1117
MroNI GCCGGC 1 cut(s) 184
MroXI GAANNNNTTC 1 cut(s) 1557
MseI TTAA 2 cut(s) 564, 750
MslI CAYNNNNRTG 2 cut(s) 819, 957
MspA1I CMGCKG 2 cut(s) 244, 300
MspR9I CCNGG 9 cut(s) 146, 263, 273, 285, 286, 1442, 1550, 1605, 1647
MunI CAATTG 1 cut(s) 689
MvaI CCWGG 4 cut(s) 263, 1550, 1605, 1647
MvnI CGCG 3 cut(s) 181, 317, 605
MwoI GCNNNNNNNGC 8 cut(s) 30, 160, 232, 241, 384, 637, 815, 1410
NaeI GCCGGC 1 cut(s) 186
NarI GGCGCC 1 cut(s) 269
NciI CCSGG 5 cut(s) 146, 273, 285, 286, 1442
NcoI CCATGG 1 cut(s) 1344
NdeII GATC 5 cut(s) 535, 556, 889, 920, 1153
NgoMIV GCCGGC 1 cut(s) 184
NheI GCTAGC 1 cut(s) 235
NmuCI GTSAC 3 cut(s) 229, 476, 599
NspI RCATGY 2 cut(s) 1474, 1586
NspV TTCGAA 1 cut(s) 587
PaeI GCATGC 1 cut(s) 1474
PciSI GCTCTTC 1 cut(s) 1755
PcsI WCGNNNNNNNCGW 8 cut(s) 62, 68, 71, 74, 86, 95, 185, 470
PdiI GCCGGC 1 cut(s) 186
PdmI GAANNNNTTC 1 cut(s) 1557
PfeI GAWTC 5 cut(s) 190, 589, 611, 698, 1697
Pfl23II CGTACG 1 cut(s) 207
PflFI GACNNNGTC 1 cut(s) 76
PinAI ACCGGT 1 cut(s) 860
PleI GAGTC 3 cut(s) 483, 1122, 1235
PluTI GGCGCC 1 cut(s) 272
PpsI GAGTC 3 cut(s) 483, 1122, 1235
PshAI GACNNNNGTC 1 cut(s) 436
PsiI TTATAA 3 cut(s) 732, 884, 1812
Psp124BI GAGCTC 1 cut(s) 656
Psp6I CCWGG 4 cut(s) 261, 1548, 1603, 1645
PspEI GGTNACC 1 cut(s) 1709
PspFI CCCAGC 1 cut(s) 240
PspGI CCWGG 4 cut(s) 261, 1548, 1603, 1645
PspLI CGTACG 1 cut(s) 207
PspOMI GGGCCC 1 cut(s) 453
PspPI GGNCC 7 cut(s) 255, 275, 280, 438, 453, 454, 1663
PsrI GAACNNNNNNTAC 1 cut(s) 34
PstNI CAGNNNCTG 2 cut(s) 121, 762
PsuI RGATCY 3 cut(s) 556, 889, 920
PsyI GACNNNGTC 1 cut(s) 76
PvuII CAGCTG 1 cut(s) 244
RsaI GTAC 9 cut(s) 5, 158, 209, 418, 766, 799, 833, 1031, 1108
RsaNI GTAC 9 cut(s) 4, 157, 208, 417, 765, 798, 832, 1030, 1107
RseI CAYNNNNRTG 2 cut(s) 819, 957
SacI GAGCTC 1 cut(s) 656
SapI GCTCTTC 1 cut(s) 1755
SaqAI TTAA 2 cut(s) 564, 750
Sau3AI GATC 5 cut(s) 535, 556, 889, 920, 1153
Sau96I GGNCC 7 cut(s) 255, 275, 280, 438, 453, 454, 1663
ScaI AGTACT 1 cut(s) 418
SchI GAGTC 3 cut(s) 484, 1123, 1235
ScrFI CCNGG 9 cut(s) 146, 263, 273, 285, 286, 1442, 1550, 1605, 1647
SduI GDGCHC 4 cut(s) 457, 656, 724, 1527
SexAI ACCWGGT 1 cut(s) 1603
SfaNI GCATC 1 cut(s) 1577
SfcI CTRYAG 1 cut(s) 1011
SfoI GGCGCC 1 cut(s) 270
SfuI TTCGAA 1 cut(s) 587
SgrAI CRCCGGYG 1 cut(s) 1371
SinI GGWCC 3 cut(s) 280, 438, 1663
SmaI CCCGGG 1 cut(s) 286
SmiMI CAYNNNNRTG 2 cut(s) 819, 957
SpeI ACTAGT 1 cut(s) 1498
SphI GCATGC 1 cut(s) 1474
Sse9I AATT 5 cut(s) 541, 583, 689, 939, 1590
SspDI GGCGCC 1 cut(s) 268
SspMI CTAG 7 cut(s) 236, 434, 1499, 1533, 1653, 1754, 1761
SstI GAGCTC 1 cut(s) 656
StyD4I CCNGG 9 cut(s) 144, 261, 271, 283, 284, 1440, 1548, 1603, 1645
StyI CCWWGG 2 cut(s) 621, 1344
TaaI ACNGT 3 cut(s) 17, 1015, 1165
TaiI ACGT 3 cut(s) 59, 80, 95
TaqI TCGA 3 cut(s) 473, 587, 1784
TasI AATT 5 cut(s) 541, 583, 689, 939, 1590
TatI WGTACW 3 cut(s) 3, 416, 1106
TauI GCSGC 6 cut(s) 184, 269, 320, 323, 1077, 1366
TfiI GAWTC 5 cut(s) 190, 589, 611, 698, 1697
Tru1I TTAA 2 cut(s) 564, 750
Tru9I TTAA 2 cut(s) 564, 750
TscAI CASTG 4 cut(s) 912, 1411, 1514, 1570
TseFI GTSAC 3 cut(s) 229, 476, 599
TseI GCWGC 4 cut(s) 24, 163, 196, 809
Tsp45I GTSAC 3 cut(s) 229, 476, 599
TspDTI ATGAA 1 cut(s) 23
TspGWI ACGGA 1 cut(s) 1833
TspMI CCCGGG 1 cut(s) 284
TspRI CASTG 4 cut(s) 912, 1411, 1514, 1570
Tth111I GACNNNGTC 1 cut(s) 76
VpaK11BI GGWCC 3 cut(s) 280, 438, 1663
XagI CCTNNNNNAGG 1 cut(s) 125
XapI RAATTY 1 cut(s) 1590
XceI RCATGY 2 cut(s) 1474, 1586
XcmI CCANNNNNNNNNTGG 1 cut(s) 945
XmaI CCCGGG 1 cut(s) 284
XmnI GAANNNNTTC 1 cut(s) 1557
XspI CTAG 7 cut(s) 236, 434, 1499, 1533, 1653, 1754, 1761
ZraI GACGTC 2 cut(s) 78, 93
ZrmI AGTACT 1 cut(s) 418
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.