RchiOBHm_Chr5g0059721

WD repeat-containing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0007846
Physical Location & Seq
Reverse (-)
64767119 .. 64771388
4270 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33631

Sequence Viewer

Length: 1854 bp
ATGTACATGAACAACGGTTACAGCAGCGCCAGCACCAAAAACGGCGTCAATCTAACGTCGTCGTCGGCGGCGGCGGCGACGACATCGGGCGCAACGACGTCGTCGTCCGGTGCGCAAACCCAGTCCCTGATAAAGGCGTATTTCAAGACCCCGGAGGGGCGGTACAAGCTGCACTCGGAGAAGACGCGGCCGGCGAATCTGCTGCCCTATTCCTACGCCAAGACAATCTCGCAGGTCACGCTAGCCCAGCTGAAGGAGAGGCCCAACCAGGCGGCGGCGGGGCCGGTCCCCGGGAACATCGCCAGCGGGAGTGTGCGCCACGCGGCGGCGAGGCTGTTGGGGGTTGGGGCTGGAAATGGGAGCAAGGCGCTTAGTTATTTGGGCGGTGGGAGTAGTAAGACGAGTAATGGAAGTAGTAGGAGTGCTCATTTTGGAGGACTAGGGTCCAATGGGTATGGGGCCCATTCGTCGCCGACTTCGAGTCACGGCGGTAAAGGGTCGTTCTTGGTGTTCAATGTTGGAGATACTATCTATATTTGCGATCTCAATTCGCCAGATAAGGATCCTATTAAAGCGTTGCATTTTAGTAATTCGAATCCGGTGTGTCACGCGTTTGATTCGGAAGCCAAGGATGGGCATGACTTGCTTATTGGATTGAGCTCTGGAGATATTTATTCAGCCTCTTTGAGACAACAATTGCAAGATTCTGGGAAGAAGCTTGTTGGTGCCCTGCATTATAACAAAGAGTGTTCTGTTAATACCAGTCGCTGTACCAGTATTGCATGGGTTCCCAAAGGCAGTGGTACTTTTATTGCTGCTCATGCTGATGGAAACTTGTACGAGTATGAGAAGGGCAAGGATAGTACCGGTGATACTTCTTTCCCAGTTATAAAGGATCCTGCTCAGTTTTCAGTGTATCATGCAAGATCCAGTAAGAGTAATCCAATTTCCAGATGGCATATTTGTGAAGGTTCAATAAACAGCATTGTTTACTCTCCGGATGGGACATATTTGGCTACAGTTGGTAGAGATGGGTACTTGCGAGTATTTGACTACTCAAAAGAACAACTGATAGGTGGTGGCAAGAGTTATTATGGTTCTCTTCTATGTTGTACTTGGAGTCCGGATGGAAAATATGTTTTGACTGGAGGGGAAGATGATCTTGTTACGGTATGGAGCATGGAAGATAGGAAGGTTGTAGCTTGGGGTGAAGGACATAACTCATGGGTTAGTGGTGTGGCTTTTGACTCGCATTGGTCATCACCAACTTCGGATGGCACAGAAGAAAATCTTGTTTATCGCTTTGGCTCTGTTGGTCAGGACACAAAACTACTCCTGTGGGAACTCTCCATGGATGAGATTGTAGTTCCGCTTCGCCGGTGTTCAGTTGGTGGCTCTCCAACATTCAGCACTGGTAGCCAGTCATCCCATTGGGACAGCAGTTACCCGGTTGGCACTCTCCAACCTGCTCCAAGCATGCGGGATGTTCCCAAGCTCTCCCCACTAGTGGCTCACTGCGTTCACACCGAGCCCCTGTCTAGTTTAGCTTTTACCCAGGAAGCTATTCTCACTGCATCTTGGGAAGGGCATGTAAAAATTTGGATGAGACCAGGCGAACCCGAAAGTAAATCCATCAACTCAGAAGCTCTGCCAGGTGCTAGTTTGAGGGACCAACTACCACCGACAGGAAAAAGTTACTCAGAATCATTGAGGGGTTACCCATTGCCTGAAAGTAAGTCAAACAACTCACAAGCTCTTCTAGGTGCTAGTTCAAAGGAGCAACAGCCATCGACGGGCAAAGGTGGTGCCTTTAGTTATAAAGACTTCTCAAAAAGCAACCGATACCTTCCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

617

Amino Acids

65.67

Weight (kDa)

8.19

Isoelectric Point (pI)

43.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WDR55 PF24796 303 - 395 8.5e-07 WDR55
Beta-prop_WDR3_1st PF25173 312 - 453 4.9e-10 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 316 - 415 1e-09 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 321 - 416 4.1e-12 THOC3 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 322 - 426 1.3e-06 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 324 - 420 1.3e-13 WDR5 beta-propeller domain
Beta-prop_EML_2 PF23414 331 - 417 6.3e-08 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 738, 890, 1818
AasI GACNNNNNNGTC 1 cut(s) 103
AatII GACGTC 1 cut(s) 101
Acc16I TGCGCA 1 cut(s) 114
Acc36I ACCTGC 2 cut(s) 223, 1474
AccB1I GGYRCC 2 cut(s) 725, 1805
AccII CGCG 3 cut(s) 187, 323, 611
AccIII TCCGGA 2 cut(s) 997, 1123
AclWI GGATC 5 cut(s) 557, 570, 890, 903, 921
AcoI YGGCCR 1 cut(s) 188
AcsI RAATTY 1 cut(s) 1596
AcuI CTGAAG 1 cut(s) 272
AcyI GRCGYC 2 cut(s) 45, 98
AfaI GTAC 8 cut(s) 5, 164, 772, 805, 839, 865, 1037, 1114
AflIII ACRYGT 1 cut(s) 609
AgeI ACCGGT 1 cut(s) 866
AgsI TTSAA 4 cut(s) 145, 514, 975, 1773
AhdI GACNNNNNGTC 1 cut(s) 480
AhlI ACTAGT 1 cut(s) 1504
AjnI CCWGG 4 cut(s) 267, 1554, 1609, 1651
Alw21I GWGCWC 2 cut(s) 427, 662
Alw26I GTCTC 2 cut(s) 682, 1600
AlwI GGATC 5 cut(s) 557, 570, 890, 903, 921
AlwNI CAGNNNCTG 2 cut(s) 127, 768
Ama87I CYCGRG 1 cut(s) 290
Aor13HI TCCGGA 2 cut(s) 997, 1123
AoxI GGCC 4 cut(s) 188, 260, 281, 459
ApaI GGGCCC 1 cut(s) 463
ApeKI GCWGC 4 cut(s) 24, 169, 202, 815
ApoI RAATTY 1 cut(s) 1596
ArsI GACNNNNNNTTYG 2 cut(s) 30, 62
AsiGI ACCGGT 1 cut(s) 866
Asp700I GAANNNNTTC 1 cut(s) 1563
AspLEI GCGC 5 cut(s) 29, 92, 115, 318, 370
AspS9I GGNCC 7 cut(s) 261, 281, 286, 444, 459, 460, 1669
AsuC2I CCSGG 4 cut(s) 152, 291, 292, 1448
AsuHPI GGTGA 3 cut(s) 881, 1220, 1254
AsuII TTCGAA 1 cut(s) 593
AsuNHI GCTAGC 1 cut(s) 241
AvaI CYCGRG 1 cut(s) 290
AvaII GGWCC 3 cut(s) 286, 444, 1669
BaeGI GKGCMC 2 cut(s) 463, 730
BamHI GGATCC 2 cut(s) 562, 895
BanI GGYRCC 2 cut(s) 725, 1805
BanII GRGCYC 3 cut(s) 463, 662, 1533
BarI GAAGNNNNNNTAC 2 cut(s) 1356, 1388
BbsI GAAGAC 1 cut(s) 188
Bbv12I GWGCWC 2 cut(s) 427, 662
BbvI GCAGC 4 cut(s) 36, 156, 189, 802
BccI CCATC 9 cut(s) 626, 821, 948, 995, 1025, 1121, 1268, 1640, 1795
BceAI ACGGC 2 cut(s) 58, 502
BcgI CGANNNNNNTGC 4 cut(s) 81, 115, 184, 218
BciT130I CCWGG 4 cut(s) 269, 1556, 1611, 1653
BcnI CCSGG 4 cut(s) 152, 291, 292, 1448
BcoDI GTCTC 2 cut(s) 682, 1600
BcuI ACTAGT 1 cut(s) 1504
BfaI CTAG 7 cut(s) 242, 440, 1505, 1539, 1659, 1760, 1767
BfmI CTRYAG 1 cut(s) 1017
BfoI RGCGCY 2 cut(s) 30, 371
BfuAI ACCTGC 2 cut(s) 223, 1474
Bme1390I CCNGG 8 cut(s) 152, 269, 291, 292, 1448, 1556, 1611, 1653
Bme18I GGWCC 3 cut(s) 286, 444, 1669
BmeRI GACNNNNNGTC 1 cut(s) 480
BmeT110I CYCGRG 1 cut(s) 290
BmgT120I GGNCC 7 cut(s) 261, 281, 286, 444, 459, 460, 1669
BmrFI CCNGG 8 cut(s) 152, 269, 291, 292, 1448, 1556, 1611, 1653
BmrI ACTGGG 2 cut(s) 115, 878
BmsI GCATC 1 cut(s) 1583
BmtI GCTAGC 1 cut(s) 245
BmuI ACTGGG 2 cut(s) 115, 878
BoxI GACNNNNGTC 1 cut(s) 442
BpiI GAAGAC 1 cut(s) 188
BpmI CTGGAG 2 cut(s) 684, 1167
Bpu14I TTCGAA 1 cut(s) 593
BpuMI CCSGG 4 cut(s) 152, 291, 292, 1448
BsaBI GATNNNNATC 1 cut(s) 561
BsaHI GRCGYC 2 cut(s) 45, 98
BsaI GGTCTC 1 cut(s) 1600
BsaJI CCNNGG 6 cut(s) 150, 289, 290, 627, 1350, 1554
BsaWI WCCGGW 5 cut(s) 107, 598, 866, 997, 1123
BsaXI ACNNNNNCTCC 2 cut(s) 146, 176
Bse118I RCCGGY 4 cut(s) 190, 283, 866, 1377
Bse1I ACTGG 8 cut(s) 121, 762, 774, 884, 930, 1150, 1417, 1420
Bse3DI GCAATG 1 cut(s) 1721
Bse8I GATNNNNATC 1 cut(s) 561
BseAI TCCGGA 2 cut(s) 997, 1123
BseBI CCWGG 4 cut(s) 269, 1556, 1611, 1653
BseDI CCNNGG 6 cut(s) 150, 289, 290, 627, 1350, 1554
BseGI GGATG 8 cut(s) 637, 1006, 1132, 1279, 1360, 1424, 1489, 1608
BseJI GATNNNNATC 1 cut(s) 561
BseMI GCAATG 1 cut(s) 1721
BseMII CTCAG 3 cut(s) 917, 1653, 1713
BseNI ACTGG 8 cut(s) 121, 762, 774, 884, 930, 1150, 1417, 1420
BseSI GKGCMC 2 cut(s) 463, 730
BseX3I CGGCCG 1 cut(s) 188
BseXI GCAGC 4 cut(s) 36, 156, 189, 802
BseYI CCCAGC 1 cut(s) 246
BsgI GTGCAG 1 cut(s) 155
Bsh1236I CGCG 3 cut(s) 187, 323, 611
Bsh1285I CGRYCG 1 cut(s) 191
BshFI GGCC 4 cut(s) 190, 262, 283, 461
BshNI GGYRCC 2 cut(s) 725, 1805
BshTI ACCGGT 1 cut(s) 866
BsiEI CGRYCG 1 cut(s) 191
BsiHKAI GWGCWC 2 cut(s) 427, 662
BsiHKCI CYCGRG 1 cut(s) 290
BslFI GGGAC 5 cut(s) 109, 272, 1018, 1448, 1682
BsmAI GTCTC 2 cut(s) 682, 1600
BsmFI GGGAC 5 cut(s) 109, 272, 1018, 1448, 1682
BsnI GGCC 4 cut(s) 190, 262, 283, 461
Bso31I GGTCTC 1 cut(s) 1600
BsoBI CYCGRG 1 cut(s) 290
Bsp119I TTCGAA 1 cut(s) 593
Bsp120I GGGCCC 1 cut(s) 459
Bsp1286I GDGCHC 5 cut(s) 427, 463, 662, 730, 1533
Bsp13I TCCGGA 2 cut(s) 997, 1123
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 5 cut(s) 541, 562, 895, 926, 1159
Bsp19I CCATGG 1 cut(s) 1350
BspANI GGCC 4 cut(s) 190, 262, 283, 461
BspCNI CTCAG 3 cut(s) 916, 1652, 1712
BspEI TCCGGA 2 cut(s) 997, 1123
BspFNI CGCG 3 cut(s) 187, 323, 611
BspMI ACCTGC 2 cut(s) 223, 1474
BspOI GCTAGC 1 cut(s) 245
BspPI GGATC 5 cut(s) 557, 570, 890, 903, 921
BspQI GCTCTTC 1 cut(s) 1761
BspT104I TTCGAA 1 cut(s) 593
BspT107I GGYRCC 2 cut(s) 725, 1805
BspTNI GGTCTC 1 cut(s) 1600
BsrDI GCAATG 1 cut(s) 1721
BsrFI RCCGGY 4 cut(s) 190, 283, 866, 1377
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 8 cut(s) 121, 762, 774, 884, 930, 1150, 1417, 1420
BssAI RCCGGY 4 cut(s) 190, 283, 866, 1377
BssECI CCNNGG 6 cut(s) 150, 289, 290, 627, 1350, 1554
BssMI GATC 5 cut(s) 541, 562, 895, 926, 1159
BssNI GRCGYC 2 cut(s) 45, 98
BssT1I CCWWGG 2 cut(s) 627, 1350
Bst2UI CCWGG 4 cut(s) 269, 1556, 1611, 1653
Bst4CI ACNGT 3 cut(s) 17, 1021, 1171
Bst6I CTCTTC 2 cut(s) 1107, 1761
BstACI GRCGYC 2 cut(s) 45, 98
BstAPI GCANNNNNTGC 1 cut(s) 643
BstAUI TGTACA 1 cut(s) 3
BstBI TTCGAA 1 cut(s) 593
BstC8I GCNNGC 5 cut(s) 31, 192, 243, 304, 1478
BstDEI CTNAG 4 cut(s) 371, 903, 1639, 1699
BstDSI CCRYGG 1 cut(s) 1350
BstEII GGTNACC 1 cut(s) 1715
BstENI CCTNNNNNAGG 1 cut(s) 131
BstF5I GGATG 8 cut(s) 637, 1006, 1132, 1279, 1360, 1424, 1489, 1608
BstFNI CGCG 3 cut(s) 187, 323, 611
BstH2I RGCGCY 2 cut(s) 30, 371
BstHHI GCGC 5 cut(s) 29, 92, 115, 318, 370
BstKTI GATC 5 cut(s) 544, 565, 898, 929, 1162
BstMAI GTCTC 2 cut(s) 682, 1600
BstMBI GATC 5 cut(s) 541, 562, 895, 926, 1159
BstMCI CGRYCG 1 cut(s) 191
BstMWI GCNNNNNNNGC 8 cut(s) 30, 74, 166, 238, 247, 643, 821, 1416
BstNI CCWGG 4 cut(s) 269, 1556, 1611, 1653
BstNSI RCATGY 2 cut(s) 1480, 1592
BstPAI GACNNNNGTC 1 cut(s) 442
BstPI GGTNACC 1 cut(s) 1715
BstSCI CCNGG 8 cut(s) 150, 267, 289, 290, 1446, 1554, 1609, 1651
BstSFI CTRYAG 1 cut(s) 1017
BstSLI GKGCMC 2 cut(s) 463, 730
BstUI CGCG 3 cut(s) 187, 323, 611
BstV1I GCAGC 4 cut(s) 36, 156, 189, 802
BstV2I GAAGAC 1 cut(s) 188
BstX2I RGATCY 3 cut(s) 562, 895, 926
BstYI RGATCY 3 cut(s) 562, 895, 926
BstZI CGGCCG 1 cut(s) 188
BsuRI GGCC 4 cut(s) 190, 262, 283, 461
BtgI CCRYGG 1 cut(s) 1350
BtgZI GCGATG 1 cut(s) 283
BtsCI GGATG 8 cut(s) 637, 1006, 1132, 1279, 1360, 1424, 1489, 1608
BtsI GCAGTG 3 cut(s) 805, 1513, 1569
BtsIMutI CAGTG 5 cut(s) 805, 918, 1410, 1513, 1569
BveI ACCTGC 2 cut(s) 223, 1474
Cac8I GCNNGC 5 cut(s) 31, 192, 243, 304, 1478
CaiI CAGNNNCTG 2 cut(s) 127, 768
CfoI GCGC 5 cut(s) 29, 92, 115, 318, 370
Cfr10I RCCGGY 4 cut(s) 190, 283, 866, 1377
Cfr13I GGNCC 7 cut(s) 261, 281, 286, 444, 459, 460, 1669
Cfr9I CCCGGG 1 cut(s) 290
CseI GACGC 2 cut(s) 34, 193
Csp6I GTAC 8 cut(s) 4, 163, 771, 804, 838, 864, 1036, 1113
CspAI ACCGGT 1 cut(s) 866
CspCI CAANNNNNGTGG 2 cut(s) 781, 816
CviQI GTAC 8 cut(s) 4, 163, 771, 804, 838, 864, 1036, 1113
DdeI CTNAG 4 cut(s) 371, 903, 1639, 1699
DpnI GATC 5 cut(s) 543, 564, 897, 928, 1161
DpnII GATC 5 cut(s) 541, 562, 895, 926, 1159
DrdI GACNNNNNNGTC 1 cut(s) 103
DriI GACNNNNNGTC 1 cut(s) 480
DseDI GACNNNNNNGTC 1 cut(s) 103
EaeI YGGCCR 1 cut(s) 188
EagI CGGCCG 1 cut(s) 188
Eam1104I CTCTTC 2 cut(s) 1107, 1761
Eam1105I GACNNNNNGTC 1 cut(s) 480
EarI CTCTTC 2 cut(s) 1107, 1761
Ecl136II GAGCTC 1 cut(s) 660
EclXI CGGCCG 1 cut(s) 188
Eco130I CCWWGG 2 cut(s) 627, 1350
Eco24I GRGCYC 3 cut(s) 463, 662, 1533
Eco31I GGTCTC 1 cut(s) 1600
Eco47I GGWCC 3 cut(s) 286, 444, 1669
Eco52I CGGCCG 1 cut(s) 188
Eco53kI GAGCTC 1 cut(s) 660
Eco57I CTGAAG 1 cut(s) 272
Eco88I CYCGRG 1 cut(s) 290
Eco91I GGTNACC 1 cut(s) 1715
EcoICRI GAGCTC 1 cut(s) 660
EcoNI CCTNNNNNAGG 1 cut(s) 131
EcoO109I RGGNCCY 1 cut(s) 459
EcoO65I GGTNACC 1 cut(s) 1715
EcoRII CCWGG 4 cut(s) 267, 1554, 1609, 1651
EcoT14I CCWWGG 2 cut(s) 627, 1350
EcoT38I GRGCYC 3 cut(s) 463, 662, 1533
ErhI CCWWGG 2 cut(s) 627, 1350
FalI AAGNNNNNCTT 4 cut(s) 1146, 1178, 1275, 1307
FaqI GGGAC 5 cut(s) 109, 272, 1018, 1448, 1682
FauI CCCGC 3 cut(s) 271, 299, 1473
FokI GGATG 8 cut(s) 644, 1013, 1139, 1286, 1367, 1411, 1496, 1615
FriOI GRGCYC 3 cut(s) 463, 662, 1533
FspBI CTAG 7 cut(s) 242, 440, 1505, 1539, 1659, 1760, 1767
FspI TGCGCA 1 cut(s) 114
GlaI GCGC 5 cut(s) 28, 91, 114, 317, 369
GsaI CCCAGC 1 cut(s) 250
GsuI CTGGAG 2 cut(s) 684, 1167
HaeII RGCGCY 2 cut(s) 30, 371
HaeIII GGCC 4 cut(s) 190, 262, 283, 461
HgaI GACGC 2 cut(s) 34, 193
HhaI GCGC 5 cut(s) 29, 92, 115, 318, 370
Hin1I GRCGYC 2 cut(s) 45, 98
Hin6I GCGC 5 cut(s) 27, 90, 113, 316, 368
HinP1I GCGC 5 cut(s) 27, 90, 113, 316, 368
HindIII AAGCTT 1 cut(s) 716
HinfI GANTC 8 cut(s) 196, 481, 595, 617, 704, 1120, 1247, 1703
HphI GGTGA 3 cut(s) 881, 1220, 1254
Hpy166II GTNNAC 2 cut(s) 991, 1522
Hpy188I TCNGA 5 cut(s) 178, 622, 1273, 1642, 1702
Hpy188III TCNNGA 6 cut(s) 145, 663, 951, 998, 1124, 1319
Hpy8I GTNNAC 2 cut(s) 991, 1522
Hpy99I CGWCG 9 cut(s) 61, 64, 67, 82, 100, 103, 106, 472, 1795
HpyAV CCTTC 6 cut(s) 247, 844, 962, 1186, 1205, 1577
HpyCH4III ACNGT 3 cut(s) 17, 1021, 1171
HpyCH4IV ACGT 2 cut(s) 56, 98
HpyCH4V TGCA 7 cut(s) 172, 580, 700, 733, 782, 923, 1574
HpyF10VI GCNNNNNNNGC 8 cut(s) 30, 74, 166, 238, 247, 643, 821, 1416
HpyF3I CTNAG 4 cut(s) 371, 903, 1639, 1699
HpySE526I ACGT 2 cut(s) 56, 98
Hsp92I GRCGYC 2 cut(s) 45, 98
HspAI GCGC 5 cut(s) 27, 90, 113, 316, 368
Kpn2I TCCGGA 2 cut(s) 997, 1123
KroI GCCGGC 1 cut(s) 190
KroNI GCCGGC 1 cut(s) 192
Kzo9I GATC 5 cut(s) 541, 562, 895, 926, 1159
LguI GCTCTTC 1 cut(s) 1761
LmnI GCTCC 4 cut(s) 360, 1176, 1474, 1777
Lsp1109I GCAGC 4 cut(s) 36, 156, 189, 802
LweI GCATC 1 cut(s) 1583
MaeI CTAG 7 cut(s) 242, 440, 1505, 1539, 1659, 1760, 1767
MaeII ACGT 2 cut(s) 56, 98
MaeIII GTNAC 8 cut(s) 17, 235, 482, 605, 1165, 1442, 1694, 1715
MalI GATC 5 cut(s) 543, 564, 897, 928, 1161
MboI GATC 5 cut(s) 541, 562, 895, 926, 1159
MboII GAAGA 7 cut(s) 193, 724, 1094, 1166, 1196, 1295, 1748
MfeI CAATTG 1 cut(s) 695
MflI RGATCY 3 cut(s) 562, 895, 926
MhlI GDGCHC 5 cut(s) 427, 463, 662, 730, 1533
MluCI AATT 5 cut(s) 547, 589, 695, 945, 1596
MluI ACGCGT 1 cut(s) 609
MlyI GAGTC 3 cut(s) 490, 1129, 1241
MmeI TCCRAC 3 cut(s) 499, 1424, 1486
MnlI CCTC 8 cut(s) 148, 252, 324, 428, 691, 1142, 1659, 1704
MroI TCCGGA 2 cut(s) 997, 1123
MroNI GCCGGC 1 cut(s) 190
MroXI GAANNNNTTC 1 cut(s) 1563
MseI TTAA 2 cut(s) 570, 756
MslI CAYNNNNRTG 2 cut(s) 825, 963
MspA1I CMGCKG 2 cut(s) 250, 306
MspR9I CCNGG 8 cut(s) 152, 269, 291, 292, 1448, 1556, 1611, 1653
MunI CAATTG 1 cut(s) 695
MvaI CCWGG 4 cut(s) 269, 1556, 1611, 1653
MvnI CGCG 3 cut(s) 187, 323, 611
MwoI GCNNNNNNNGC 8 cut(s) 30, 74, 166, 238, 247, 643, 821, 1416
NaeI GCCGGC 1 cut(s) 192
NciI CCSGG 4 cut(s) 152, 291, 292, 1448
NcoI CCATGG 1 cut(s) 1350
NdeII GATC 5 cut(s) 541, 562, 895, 926, 1159
NgoMIV GCCGGC 1 cut(s) 190
NheI GCTAGC 1 cut(s) 241
NmuCI GTSAC 3 cut(s) 235, 482, 605
NsbI TGCGCA 1 cut(s) 114
NspI RCATGY 2 cut(s) 1480, 1592
NspV TTCGAA 1 cut(s) 593
PaeI GCATGC 1 cut(s) 1480
PciSI GCTCTTC 1 cut(s) 1761
PcsI WCGNNNNNNNCGW 4 cut(s) 92, 101, 191, 476
PdiI GCCGGC 1 cut(s) 192
PdmI GAANNNNTTC 1 cut(s) 1563
PfeI GAWTC 5 cut(s) 196, 595, 617, 704, 1703
PflFI GACNNNGTC 1 cut(s) 100
PinAI ACCGGT 1 cut(s) 866
PleI GAGTC 3 cut(s) 489, 1128, 1241
PpsI GAGTC 3 cut(s) 489, 1128, 1241
PshAI GACNNNNGTC 1 cut(s) 442
PsiI TTATAA 3 cut(s) 738, 890, 1818
Psp124BI GAGCTC 1 cut(s) 662
Psp6I CCWGG 4 cut(s) 267, 1554, 1609, 1651
PspEI GGTNACC 1 cut(s) 1715
PspFI CCCAGC 1 cut(s) 246
PspGI CCWGG 4 cut(s) 267, 1554, 1609, 1651
PspOMI GGGCCC 1 cut(s) 459
PspPI GGNCC 7 cut(s) 261, 281, 286, 444, 459, 460, 1669
PsrI GAACNNNNNNTAC 1 cut(s) 34
PstNI CAGNNNCTG 2 cut(s) 127, 768
PsuI RGATCY 3 cut(s) 562, 895, 926
PsyI GACNNNGTC 1 cut(s) 100
PvuII CAGCTG 1 cut(s) 250
RsaI GTAC 8 cut(s) 5, 164, 772, 805, 839, 865, 1037, 1114
RsaNI GTAC 8 cut(s) 4, 163, 771, 804, 838, 864, 1036, 1113
RseI CAYNNNNRTG 2 cut(s) 825, 963
SacI GAGCTC 1 cut(s) 662
SapI GCTCTTC 1 cut(s) 1761
SaqAI TTAA 2 cut(s) 570, 756
Sau3AI GATC 5 cut(s) 541, 562, 895, 926, 1159
Sau96I GGNCC 7 cut(s) 261, 281, 286, 444, 459, 460, 1669
SchI GAGTC 3 cut(s) 490, 1129, 1241
ScrFI CCNGG 8 cut(s) 152, 269, 291, 292, 1448, 1556, 1611, 1653
SduI GDGCHC 5 cut(s) 427, 463, 662, 730, 1533
SfaNI GCATC 1 cut(s) 1583
SfcI CTRYAG 1 cut(s) 1017
SfuI TTCGAA 1 cut(s) 593
SgrAI CRCCGGYG 1 cut(s) 1377
SinI GGWCC 3 cut(s) 286, 444, 1669
SmaI CCCGGG 1 cut(s) 292
SmiMI CAYNNNNRTG 2 cut(s) 825, 963
SpeI ACTAGT 1 cut(s) 1504
SphI GCATGC 1 cut(s) 1480
Sse9I AATT 5 cut(s) 547, 589, 695, 945, 1596
SspMI CTAG 7 cut(s) 242, 440, 1505, 1539, 1659, 1760, 1767
SstI GAGCTC 1 cut(s) 662
StyD4I CCNGG 8 cut(s) 150, 267, 289, 290, 1446, 1554, 1609, 1651
StyI CCWWGG 2 cut(s) 627, 1350
TaaI ACNGT 3 cut(s) 17, 1021, 1171
TaiI ACGT 2 cut(s) 59, 101
TaqI TCGA 3 cut(s) 479, 593, 1790
TasI AATT 5 cut(s) 547, 589, 695, 945, 1596
TatI WGTACW 2 cut(s) 3, 1112
TauI GCSGC 8 cut(s) 71, 74, 77, 190, 275, 278, 326, 329
TfiI GAWTC 5 cut(s) 196, 595, 617, 704, 1703
Tru1I TTAA 2 cut(s) 570, 756
Tru9I TTAA 2 cut(s) 570, 756
TscAI CASTG 5 cut(s) 805, 918, 1417, 1520, 1576
TseFI GTSAC 3 cut(s) 235, 482, 605
TseI GCWGC 4 cut(s) 24, 169, 202, 815
Tsp45I GTSAC 3 cut(s) 235, 482, 605
TspDTI ATGAA 1 cut(s) 23
TspGWI ACGGA 1 cut(s) 1839
TspMI CCCGGG 1 cut(s) 290
TspRI CASTG 5 cut(s) 805, 918, 1417, 1520, 1576
Tth111I GACNNNGTC 1 cut(s) 100
VpaK11BI GGWCC 3 cut(s) 286, 444, 1669
XagI CCTNNNNNAGG 1 cut(s) 131
XapI RAATTY 1 cut(s) 1596
XceI RCATGY 2 cut(s) 1480, 1592
XcmI CCANNNNNNNNNTGG 1 cut(s) 951
XmaI CCCGGG 1 cut(s) 290
XmnI GAANNNNTTC 1 cut(s) 1563
XspI CTAG 7 cut(s) 242, 440, 1505, 1539, 1659, 1760, 1767
ZraI GACGTC 1 cut(s) 99
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.