Rh5AG389900

WD repeat-containing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0007846
Physical Location & Seq
Reverse (-)
67433027 .. 67436766
3740 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG389900.1

Sequence Viewer

Length: 1845 bp
ATGTACATTAACAACGGTTACAGCAGCGCCAGCACCAAAAACGGCGTCAATCTAACGTCGTCGGCGGCGACGACATCGGGCGCAACGACGTCGTCGTCCGGTGCGCAAACCCAGTCCCTGATAAAGGCGTATTTCAAGACCCCCGAGGGGCGGTACAAGCTGCACTCGGAGAAGACCCGGCCGGCGAATCTGCTGCCCTATTCGTACGCCAAGACAATCTCGCAGGTCACGCTAGCCCAGCTGAAGGAGAGGCCCAACCAGGCGGCGCCGGGGCCGGTCCCCGGGAACATCGCCAGCGGGAGTGTGCGCCACGCGGCCGCGAGGCTGTTGGGGGTTGGGGCTGGAAATGGGAGCAAGGCGCTTAGTTATTTGGGCGGTGGGAGTAGTAAGACGAGTAATGGAAGTAGTAGGAGTGCTCATTTTGGAGGACTAGGGTCCAATGGGTATGGGGCCCATTCGTCGCCGACTTCGAGTCACGGCGGTAAAGGGTCGTTCTTGGTGTTCAATGTTGGAGATACTATCTATATTTGCGATCTCAATTCGCCAGATAAGGATCCTATTAAAGCGTTGCATTTTAGTAATTCGAATCCGGTGTGTCACGCGTTTGATTCGGAAGCCAAGGATGGGCATGACTTGCTTATTGGATTGAGCTCTGGAGATGTTTATTCAGCCTCTTTGAGACAGCAATTGCAAGATTCTGGGAAGAAGCTTGTTGGTGCCCTGCATTATAACAAAGAGGGTTCTGTTAATACCAGTCGCTGTACCAGTATTGCATGGGTTCCCAAAGGCAATGGTACTTTTATTGCTGCTCATGCTGATGGAAACTTGTACGAGTATGAGAAGGGCAAGGATATTACCGGTGATACTTCTTTCCCAGTTATAAAGGATCCTGCGCAGTTTTCAGTGTATCATGCAAGATCCAGTAAGAGTAATCCAATTTCCAGATGGCATATTTGTGAAGGTTCAATAAACAGCATTGTTTACTCTCCGGATGGGACATATTTGGCTACAGTTGGTAGAGATGGGTACTTGCGAGTATTTGACTACTCAAAAGAACAACTGATAGGTGGCGGCAAGAGTTATTATGGTTCTCTTCTATGTTGTACTTGGAGTCCGGATGGAAAATATGTTTTGACTGGAGGGGAAGATGATCTTGTTACAGTATGGAGCATGGAAGATAGGAAGGTTGTAGCTTGGGGTGAAGGACATAACTCATGGGTTAGTGGTGTGGCTTTTGACTCGCATTGGTCATCACCAACTTCGGATGGCACAGAAGAAAATCTTGTTTATCGCTTTGGCTCTGTTGGTCAGGACACAAAACTACTCCTGTGGGAACTCTCCATGGATGAGATTGTAGTGCCGCTTCGCCGGTGTTCAGTTGGTGGCTCTCCAACATTCAGCACTGGTAGCCAGTCATCCCATTGGGACAGCAGTTACCCGGTTGGCACTCTCCAACCTGCTCCAAGCATGCGGGATGTTCCCAAGCTCTCCCCACTAGTGGCTCACTGCGTTCACACCGAGCCCCTGTCTAGTTTAGCTTTTACCCAGGAAGCTATTCTCACTGCATCTTGGGAAGGGCATGTAAAAATTTGGATGAGACCAGGCGAACCCGAAAGTAAATCCACCAACTCAGAAGCTCTGCCAGGTGCTAGTTTGAGGGACCAACTACCACCGACAGGAAAAAGTTACTCAGAATCATTGAGGGGTTACCCATTGCCTGAAAGTAAATCAAACAACTCACAAGCTCTTCTAGGTGCTAGTTCAAAGGAGCAACAGCCATCGACGGGCAAAGCTGGTGCCTTTAGTTATAAAGACTTCTCAAAAAGCAACCGATACCTTCCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

614

Amino Acids

65.44

Weight (kDa)

8.22

Isoelectric Point (pI)

41.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WDR55 PF24796 301 - 392 9e-07 WDR55
Beta-prop_WDR3_1st PF25173 309 - 450 4.9e-10 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 313 - 412 1.2e-09 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 318 - 413 4.1e-12 THOC3 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 319 - 423 1.3e-06 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 321 - 417 1.3e-13 WDR5 beta-propeller domain
Beta-prop_EML_2 PF23414 328 - 414 6.3e-08 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 729, 881, 1809
AasI GACNNNNNNGTC 1 cut(s) 94
AatII GACGTC 1 cut(s) 92
Acc16I TGCGCA 2 cut(s) 105, 894
Acc36I ACCTGC 2 cut(s) 214, 1465
AccB1I GGYRCC 3 cut(s) 265, 716, 1796
AccII CGCG 3 cut(s) 314, 320, 602
AccIII TCCGGA 2 cut(s) 988, 1114
AclWI GGATC 5 cut(s) 548, 561, 881, 894, 912
AcoI YGGCCR 2 cut(s) 179, 315
AcsI RAATTY 1 cut(s) 1587
AcuI CTGAAG 1 cut(s) 263
AcyI GRCGYC 3 cut(s) 45, 89, 266
AfaI GTAC 8 cut(s) 5, 155, 206, 763, 796, 830, 1028, 1105
AfiI CCNNNNNNNGG 9 cut(s) 124, 147, 150, 244, 281, 624, 1498, 1676, 1784
AflIII ACRYGT 1 cut(s) 600
AgeI ACCGGT 1 cut(s) 857
AgsI TTSAA 4 cut(s) 136, 505, 966, 1764
AhdI GACNNNNNGTC 1 cut(s) 471
AhlI ACTAGT 1 cut(s) 1495
AjnI CCWGG 4 cut(s) 258, 1545, 1600, 1642
Alw21I GWGCWC 2 cut(s) 418, 653
Alw26I GTCTC 2 cut(s) 673, 1591
AlwI GGATC 5 cut(s) 548, 561, 881, 894, 912
AlwNI CAGNNNCTG 2 cut(s) 118, 759
Ama87I CYCGRG 2 cut(s) 143, 281
Aor13HI TCCGGA 2 cut(s) 988, 1114
AoxI GGCC 5 cut(s) 179, 251, 272, 315, 450
ApaI GGGCCC 1 cut(s) 454
ApeKI GCWGC 4 cut(s) 24, 160, 193, 806
ApoI RAATTY 1 cut(s) 1587
ArsI GACNNNNNNTTYG 2 cut(s) 30, 62
AsiGI ACCGGT 1 cut(s) 857
Asp700I GAANNNNTTC 1 cut(s) 1554
AspLEI GCGC 7 cut(s) 29, 83, 106, 268, 309, 361, 895
AspS9I GGNCC 7 cut(s) 252, 272, 277, 435, 450, 451, 1660
AsuC2I CCSGG 5 cut(s) 178, 270, 282, 283, 1439
AsuHPI GGTGA 3 cut(s) 872, 1211, 1245
AsuII TTCGAA 1 cut(s) 584
AsuNHI GCTAGC 1 cut(s) 232
AvaI CYCGRG 2 cut(s) 143, 281
AvaII GGWCC 3 cut(s) 277, 435, 1660
BaeGI GKGCMC 2 cut(s) 454, 721
BamHI GGATCC 2 cut(s) 553, 886
BanI GGYRCC 3 cut(s) 265, 716, 1796
BanII GRGCYC 3 cut(s) 454, 653, 1524
BarI GAAGNNNNNNTAC 2 cut(s) 1347, 1379
BbsI GAAGAC 1 cut(s) 179
Bbv12I GWGCWC 2 cut(s) 418, 653
BbvI GCAGC 4 cut(s) 36, 147, 180, 793
BccI CCATC 8 cut(s) 617, 812, 939, 986, 1016, 1112, 1259, 1786
BceAI ACGGC 2 cut(s) 58, 493
BcgI CGANNNNNNTGC 4 cut(s) 72, 106, 175, 209
BciT130I CCWGG 4 cut(s) 260, 1547, 1602, 1644
BcnI CCSGG 5 cut(s) 178, 270, 282, 283, 1439
BcoDI GTCTC 2 cut(s) 673, 1591
BcuI ACTAGT 1 cut(s) 1495
BfaI CTAG 7 cut(s) 233, 431, 1496, 1530, 1650, 1751, 1758
BfmI CTRYAG 1 cut(s) 1008
BfoI RGCGCY 3 cut(s) 30, 269, 362
BfuAI ACCTGC 2 cut(s) 214, 1465
Bme1390I CCNGG 9 cut(s) 178, 260, 270, 282, 283, 1439, 1547, 1602, 1644
Bme18I GGWCC 3 cut(s) 277, 435, 1660
BmeRI GACNNNNNGTC 1 cut(s) 471
BmeT110I CYCGRG 2 cut(s) 143, 281
BmgT120I GGNCC 7 cut(s) 252, 272, 277, 435, 450, 451, 1660
BmrFI CCNGG 9 cut(s) 178, 260, 270, 282, 283, 1439, 1547, 1602, 1644
BmrI ACTGGG 2 cut(s) 106, 869
BmsI GCATC 1 cut(s) 1574
BmtI GCTAGC 1 cut(s) 236
BmuI ACTGGG 2 cut(s) 106, 869
BoxI GACNNNNGTC 1 cut(s) 433
BpiI GAAGAC 1 cut(s) 179
BpmI CTGGAG 2 cut(s) 675, 1158
Bpu14I TTCGAA 1 cut(s) 584
BpuMI CCSGG 5 cut(s) 178, 270, 282, 283, 1439
BsaBI GATNNNNATC 1 cut(s) 552
BsaHI GRCGYC 3 cut(s) 45, 89, 266
BsaI GGTCTC 1 cut(s) 1591
BsaJI CCNNGG 7 cut(s) 144, 269, 280, 281, 618, 1341, 1545
BsaWI WCCGGW 5 cut(s) 98, 589, 857, 988, 1114
Bsc4I CCNNNNNNNGG 9 cut(s) 124, 147, 150, 244, 281, 624, 1498, 1676, 1784
Bse118I RCCGGY 4 cut(s) 181, 274, 857, 1368
Bse1I ACTGG 8 cut(s) 112, 753, 765, 875, 921, 1141, 1408, 1411
Bse3DI GCAATG 2 cut(s) 796, 1712
Bse8I GATNNNNATC 1 cut(s) 552
BseAI TCCGGA 2 cut(s) 988, 1114
BseBI CCWGG 4 cut(s) 260, 1547, 1602, 1644
BseDI CCNNGG 7 cut(s) 144, 269, 280, 281, 618, 1341, 1545
BseGI GGATG 8 cut(s) 628, 997, 1123, 1270, 1351, 1415, 1480, 1599
BseJI GATNNNNATC 1 cut(s) 552
BseLI CCNNNNNNNGG 9 cut(s) 124, 147, 150, 244, 281, 624, 1498, 1676, 1784
BseMI GCAATG 2 cut(s) 796, 1712
BseMII CTCAG 2 cut(s) 1644, 1704
BseNI ACTGG 8 cut(s) 112, 753, 765, 875, 921, 1141, 1408, 1411
BseSI GKGCMC 2 cut(s) 454, 721
BseX3I CGGCCG 2 cut(s) 179, 315
BseXI GCAGC 4 cut(s) 36, 147, 180, 793
BseYI CCCAGC 1 cut(s) 237
BsgI GTGCAG 1 cut(s) 146
Bsh1236I CGCG 3 cut(s) 314, 320, 602
Bsh1285I CGRYCG 2 cut(s) 182, 318
BshFI GGCC 5 cut(s) 181, 253, 274, 317, 452
BshNI GGYRCC 3 cut(s) 265, 716, 1796
BshTI ACCGGT 1 cut(s) 857
BsiEI CGRYCG 2 cut(s) 182, 318
BsiHKAI GWGCWC 2 cut(s) 418, 653
BsiHKCI CYCGRG 2 cut(s) 143, 281
BsiWI CGTACG 1 cut(s) 204
BslFI GGGAC 5 cut(s) 100, 263, 1009, 1439, 1673
BslI CCNNNNNNNGG 9 cut(s) 124, 147, 150, 244, 281, 624, 1498, 1676, 1784
BsmAI GTCTC 2 cut(s) 673, 1591
BsmFI GGGAC 5 cut(s) 100, 263, 1009, 1439, 1673
BsnI GGCC 5 cut(s) 181, 253, 274, 317, 452
Bso31I GGTCTC 1 cut(s) 1591
BsoBI CYCGRG 2 cut(s) 143, 281
Bsp119I TTCGAA 1 cut(s) 584
Bsp120I GGGCCC 1 cut(s) 450
Bsp1286I GDGCHC 5 cut(s) 418, 454, 653, 721, 1524
Bsp13I TCCGGA 2 cut(s) 988, 1114
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 5 cut(s) 532, 553, 886, 917, 1150
Bsp19I CCATGG 1 cut(s) 1341
BspANI GGCC 5 cut(s) 181, 253, 274, 317, 452
BspCNI CTCAG 2 cut(s) 1643, 1703
BspEI TCCGGA 2 cut(s) 988, 1114
BspFNI CGCG 3 cut(s) 314, 320, 602
BspMI ACCTGC 2 cut(s) 214, 1465
BspOI GCTAGC 1 cut(s) 236
BspPI GGATC 5 cut(s) 548, 561, 881, 894, 912
BspQI GCTCTTC 1 cut(s) 1752
BspT104I TTCGAA 1 cut(s) 584
BspT107I GGYRCC 3 cut(s) 265, 716, 1796
BspTNI GGTCTC 1 cut(s) 1591
BsrDI GCAATG 2 cut(s) 796, 1712
BsrFI RCCGGY 4 cut(s) 181, 274, 857, 1368
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 8 cut(s) 112, 753, 765, 875, 921, 1141, 1408, 1411
BssAI RCCGGY 4 cut(s) 181, 274, 857, 1368
BssECI CCNNGG 7 cut(s) 144, 269, 280, 281, 618, 1341, 1545
BssMI GATC 5 cut(s) 532, 553, 886, 917, 1150
BssNI GRCGYC 3 cut(s) 45, 89, 266
BssT1I CCWWGG 2 cut(s) 618, 1341
Bst2UI CCWGG 4 cut(s) 260, 1547, 1602, 1644
Bst4CI ACNGT 3 cut(s) 17, 1012, 1162
Bst6I CTCTTC 2 cut(s) 1098, 1752
BstACI GRCGYC 3 cut(s) 45, 89, 266
BstAPI GCANNNNNTGC 1 cut(s) 634
BstAUI TGTACA 1 cut(s) 3
BstBI TTCGAA 1 cut(s) 584
BstC8I GCNNGC 5 cut(s) 31, 183, 234, 295, 1469
BstDEI CTNAG 3 cut(s) 362, 1630, 1690
BstDSI CCRYGG 1 cut(s) 1341
BstEII GGTNACC 1 cut(s) 1706
BstENI CCTNNNNNAGG 1 cut(s) 122
BstF5I GGATG 8 cut(s) 628, 997, 1123, 1270, 1351, 1415, 1480, 1599
BstFNI CGCG 3 cut(s) 314, 320, 602
BstH2I RGCGCY 3 cut(s) 30, 269, 362
BstHHI GCGC 7 cut(s) 29, 83, 106, 268, 309, 361, 895
BstKTI GATC 5 cut(s) 535, 556, 889, 920, 1153
BstMAI GTCTC 2 cut(s) 673, 1591
BstMBI GATC 5 cut(s) 532, 553, 886, 917, 1150
BstMCI CGRYCG 2 cut(s) 182, 318
BstMWI GCNNNNNNNGC 7 cut(s) 30, 157, 229, 238, 634, 812, 1407
BstNI CCWGG 4 cut(s) 260, 1547, 1602, 1644
BstNSI RCATGY 2 cut(s) 1471, 1583
BstPAI GACNNNNGTC 1 cut(s) 433
BstPI GGTNACC 1 cut(s) 1706
BstSCI CCNGG 9 cut(s) 176, 258, 268, 280, 281, 1437, 1545, 1600, 1642
BstSFI CTRYAG 1 cut(s) 1008
BstSLI GKGCMC 2 cut(s) 454, 721
BstUI CGCG 3 cut(s) 314, 320, 602
BstV1I GCAGC 4 cut(s) 36, 147, 180, 793
BstV2I GAAGAC 1 cut(s) 179
BstX2I RGATCY 3 cut(s) 553, 886, 917
BstYI RGATCY 3 cut(s) 553, 886, 917
BstZI CGGCCG 2 cut(s) 179, 315
BsuRI GGCC 5 cut(s) 181, 253, 274, 317, 452
BtgI CCRYGG 1 cut(s) 1341
BtgZI GCGATG 1 cut(s) 274
BtsCI GGATG 8 cut(s) 628, 997, 1123, 1270, 1351, 1415, 1480, 1599
BtsI GCAGTG 2 cut(s) 1504, 1560
BtsIMutI CAGTG 4 cut(s) 909, 1401, 1504, 1560
BveI ACCTGC 2 cut(s) 214, 1465
Cac8I GCNNGC 5 cut(s) 31, 183, 234, 295, 1469
CaiI CAGNNNCTG 2 cut(s) 118, 759
CciNI GCGGCCGC 1 cut(s) 315
CfoI GCGC 7 cut(s) 29, 83, 106, 268, 309, 361, 895
Cfr10I RCCGGY 4 cut(s) 181, 274, 857, 1368
Cfr13I GGNCC 7 cut(s) 252, 272, 277, 435, 450, 451, 1660
Cfr9I CCCGGG 1 cut(s) 281
CseI GACGC 1 cut(s) 34
Csp6I GTAC 8 cut(s) 4, 154, 205, 762, 795, 829, 1027, 1104
CspAI ACCGGT 1 cut(s) 857
CviAII CATG 9 cut(s) 629, 774, 812, 911, 1171, 1215, 1342, 1468, 1580
CviQI GTAC 8 cut(s) 4, 154, 205, 762, 795, 829, 1027, 1104
DdeI CTNAG 3 cut(s) 362, 1630, 1690
DinI GGCGCC 1 cut(s) 267
DpnI GATC 5 cut(s) 534, 555, 888, 919, 1152
DpnII GATC 5 cut(s) 532, 553, 886, 917, 1150
DrdI GACNNNNNNGTC 1 cut(s) 94
DriI GACNNNNNGTC 1 cut(s) 471
DseDI GACNNNNNNGTC 1 cut(s) 94
EaeI YGGCCR 2 cut(s) 179, 315
EagI CGGCCG 2 cut(s) 179, 315
Eam1104I CTCTTC 2 cut(s) 1098, 1752
Eam1105I GACNNNNNGTC 1 cut(s) 471
EarI CTCTTC 2 cut(s) 1098, 1752
Ecl136II GAGCTC 1 cut(s) 651
EclXI CGGCCG 2 cut(s) 179, 315
Eco130I CCWWGG 2 cut(s) 618, 1341
Eco24I GRGCYC 3 cut(s) 454, 653, 1524
Eco31I GGTCTC 1 cut(s) 1591
Eco47I GGWCC 3 cut(s) 277, 435, 1660
Eco52I CGGCCG 2 cut(s) 179, 315
Eco53kI GAGCTC 1 cut(s) 651
Eco57I CTGAAG 1 cut(s) 263
Eco88I CYCGRG 2 cut(s) 143, 281
Eco91I GGTNACC 1 cut(s) 1706
EcoICRI GAGCTC 1 cut(s) 651
EcoNI CCTNNNNNAGG 1 cut(s) 122
EcoO109I RGGNCCY 1 cut(s) 450
EcoO65I GGTNACC 1 cut(s) 1706
EcoRII CCWGG 4 cut(s) 258, 1545, 1600, 1642
EcoT14I CCWWGG 2 cut(s) 618, 1341
EcoT38I GRGCYC 3 cut(s) 454, 653, 1524
EgeI GGCGCC 1 cut(s) 267
EheI GGCGCC 1 cut(s) 267
ErhI CCWWGG 2 cut(s) 618, 1341
FaeI CATG 9 cut(s) 632, 777, 815, 914, 1174, 1218, 1345, 1471, 1583
FalI AAGNNNNNCTT 4 cut(s) 1137, 1169, 1266, 1298
FaqI GGGAC 5 cut(s) 100, 263, 1009, 1439, 1673
FatI CATG 9 cut(s) 628, 773, 811, 910, 1170, 1214, 1341, 1467, 1579
FauI CCCGC 2 cut(s) 290, 1464
FokI GGATG 8 cut(s) 635, 1004, 1130, 1277, 1358, 1402, 1487, 1606
FriOI GRGCYC 3 cut(s) 454, 653, 1524
FspBI CTAG 7 cut(s) 233, 431, 1496, 1530, 1650, 1751, 1758
FspI TGCGCA 2 cut(s) 105, 894
GlaI GCGC 7 cut(s) 28, 82, 105, 267, 308, 360, 894
GsaI CCCAGC 1 cut(s) 241
GsuI CTGGAG 2 cut(s) 675, 1158
HaeII RGCGCY 3 cut(s) 30, 269, 362
HaeIII GGCC 5 cut(s) 181, 253, 274, 317, 452
HgaI GACGC 1 cut(s) 34
HhaI GCGC 7 cut(s) 29, 83, 106, 268, 309, 361, 895
Hin1I GRCGYC 3 cut(s) 45, 89, 266
Hin1II CATG 9 cut(s) 632, 777, 815, 914, 1174, 1218, 1345, 1471, 1583
Hin6I GCGC 7 cut(s) 27, 81, 104, 266, 307, 359, 893
HinP1I GCGC 7 cut(s) 27, 81, 104, 266, 307, 359, 893
HindIII AAGCTT 1 cut(s) 707
HinfI GANTC 8 cut(s) 187, 472, 586, 608, 695, 1111, 1238, 1694
HphI GGTGA 3 cut(s) 872, 1211, 1245
Hpy166II GTNNAC 2 cut(s) 982, 1513
Hpy188I TCNGA 5 cut(s) 169, 613, 1264, 1633, 1693
Hpy188III TCNNGA 6 cut(s) 136, 654, 942, 989, 1115, 1310
Hpy8I GTNNAC 2 cut(s) 982, 1513
Hpy99I CGWCG 8 cut(s) 61, 64, 73, 91, 94, 97, 463, 1786
HpyAV CCTTC 6 cut(s) 238, 835, 953, 1177, 1196, 1568
HpyCH4III ACNGT 3 cut(s) 17, 1012, 1162
HpyCH4IV ACGT 2 cut(s) 56, 89
HpyCH4V TGCA 7 cut(s) 163, 571, 691, 724, 773, 914, 1565
HpyF10VI GCNNNNNNNGC 7 cut(s) 30, 157, 229, 238, 634, 812, 1407
HpyF3I CTNAG 3 cut(s) 362, 1630, 1690
HpySE526I ACGT 2 cut(s) 56, 89
Hsp92I GRCGYC 3 cut(s) 45, 89, 266
Hsp92II CATG 9 cut(s) 632, 777, 815, 914, 1174, 1218, 1345, 1471, 1583
HspAI GCGC 7 cut(s) 27, 81, 104, 266, 307, 359, 893
KasI GGCGCC 1 cut(s) 265
Kpn2I TCCGGA 2 cut(s) 988, 1114
KroI GCCGGC 1 cut(s) 181
KroNI GCCGGC 1 cut(s) 183
Kzo9I GATC 5 cut(s) 532, 553, 886, 917, 1150
LguI GCTCTTC 1 cut(s) 1752
LmnI GCTCC 4 cut(s) 351, 1167, 1465, 1768
Lsp1109I GCAGC 4 cut(s) 36, 147, 180, 793
LweI GCATC 1 cut(s) 1574
MaeI CTAG 7 cut(s) 233, 431, 1496, 1530, 1650, 1751, 1758
MaeII ACGT 2 cut(s) 56, 89
MaeIII GTNAC 8 cut(s) 17, 226, 473, 596, 1156, 1433, 1685, 1706
MalI GATC 5 cut(s) 534, 555, 888, 919, 1152
MboI GATC 5 cut(s) 532, 553, 886, 917, 1150
MboII GAAGA 7 cut(s) 184, 715, 1085, 1157, 1187, 1286, 1739
MfeI CAATTG 1 cut(s) 686
MflI RGATCY 3 cut(s) 553, 886, 917
MhlI GDGCHC 5 cut(s) 418, 454, 653, 721, 1524
MluCI AATT 5 cut(s) 538, 580, 686, 936, 1587
MluI ACGCGT 1 cut(s) 600
Mly113I GGCGCC 1 cut(s) 266
MlyI GAGTC 3 cut(s) 481, 1120, 1232
MmeI TCCRAC 3 cut(s) 490, 1415, 1477
MnlI CCTC 9 cut(s) 139, 243, 315, 419, 682, 730, 1133, 1650, 1695
MroI TCCGGA 2 cut(s) 988, 1114
MroNI GCCGGC 1 cut(s) 181
MroXI GAANNNNTTC 1 cut(s) 1554
MseI TTAA 3 cut(s) 9, 561, 747
MslI CAYNNNNRTG 2 cut(s) 816, 954
MspA1I CMGCKG 2 cut(s) 241, 297
MspR9I CCNGG 9 cut(s) 178, 260, 270, 282, 283, 1439, 1547, 1602, 1644
MunI CAATTG 1 cut(s) 686
MvaI CCWGG 4 cut(s) 260, 1547, 1602, 1644
MvnI CGCG 3 cut(s) 314, 320, 602
MwoI GCNNNNNNNGC 7 cut(s) 30, 157, 229, 238, 634, 812, 1407
NaeI GCCGGC 1 cut(s) 183
NarI GGCGCC 1 cut(s) 266
NciI CCSGG 5 cut(s) 178, 270, 282, 283, 1439
NcoI CCATGG 1 cut(s) 1341
NdeII GATC 5 cut(s) 532, 553, 886, 917, 1150
NgoMIV GCCGGC 1 cut(s) 181
NheI GCTAGC 1 cut(s) 232
NlaIII CATG 9 cut(s) 632, 777, 815, 914, 1174, 1218, 1345, 1471, 1583
NmuCI GTSAC 3 cut(s) 226, 473, 596
NotI GCGGCCGC 1 cut(s) 315
NsbI TGCGCA 2 cut(s) 105, 894
NspI RCATGY 2 cut(s) 1471, 1583
NspV TTCGAA 1 cut(s) 584
PaeI GCATGC 1 cut(s) 1471
PciSI GCTCTTC 1 cut(s) 1752
PcsI WCGNNNNNNNCGW 5 cut(s) 65, 68, 83, 92, 467
PdiI GCCGGC 1 cut(s) 183
PdmI GAANNNNTTC 1 cut(s) 1554
PfeI GAWTC 5 cut(s) 187, 586, 608, 695, 1694
Pfl23II CGTACG 1 cut(s) 204
PflFI GACNNNGTC 1 cut(s) 91
PinAI ACCGGT 1 cut(s) 857
PleI GAGTC 3 cut(s) 480, 1119, 1232
PluTI GGCGCC 1 cut(s) 269
PpsI GAGTC 3 cut(s) 480, 1119, 1232
PshAI GACNNNNGTC 1 cut(s) 433
PsiI TTATAA 3 cut(s) 729, 881, 1809
Psp124BI GAGCTC 1 cut(s) 653
Psp6I CCWGG 4 cut(s) 258, 1545, 1600, 1642
PspEI GGTNACC 1 cut(s) 1706
PspFI CCCAGC 1 cut(s) 237
PspGI CCWGG 4 cut(s) 258, 1545, 1600, 1642
PspLI CGTACG 1 cut(s) 204
PspOMI GGGCCC 1 cut(s) 450
PspPI GGNCC 7 cut(s) 252, 272, 277, 435, 450, 451, 1660
PstNI CAGNNNCTG 2 cut(s) 118, 759
PsuI RGATCY 3 cut(s) 553, 886, 917
PsyI GACNNNGTC 1 cut(s) 91
PvuII CAGCTG 1 cut(s) 241
RsaI GTAC 8 cut(s) 5, 155, 206, 763, 796, 830, 1028, 1105
RsaNI GTAC 8 cut(s) 4, 154, 205, 762, 795, 829, 1027, 1104
RseI CAYNNNNRTG 2 cut(s) 816, 954
SacI GAGCTC 1 cut(s) 653
SapI GCTCTTC 1 cut(s) 1752
SaqAI TTAA 3 cut(s) 9, 561, 747
Sau3AI GATC 5 cut(s) 532, 553, 886, 917, 1150
Sau96I GGNCC 7 cut(s) 252, 272, 277, 435, 450, 451, 1660
SchI GAGTC 3 cut(s) 481, 1120, 1232
ScrFI CCNGG 9 cut(s) 178, 260, 270, 282, 283, 1439, 1547, 1602, 1644
SduI GDGCHC 5 cut(s) 418, 454, 653, 721, 1524
SfaNI GCATC 1 cut(s) 1574
SfcI CTRYAG 1 cut(s) 1008
SfoI GGCGCC 1 cut(s) 267
SfuI TTCGAA 1 cut(s) 584
SgrAI CRCCGGYG 1 cut(s) 1368
SinI GGWCC 3 cut(s) 277, 435, 1660
SmaI CCCGGG 1 cut(s) 283
SmiMI CAYNNNNRTG 2 cut(s) 816, 954
SpeI ACTAGT 1 cut(s) 1495
SphI GCATGC 1 cut(s) 1471
Sse9I AATT 5 cut(s) 538, 580, 686, 936, 1587
SspDI GGCGCC 1 cut(s) 265
SspMI CTAG 7 cut(s) 233, 431, 1496, 1530, 1650, 1751, 1758
SstI GAGCTC 1 cut(s) 653
StyD4I CCNGG 9 cut(s) 176, 258, 268, 280, 281, 1437, 1545, 1600, 1642
StyI CCWWGG 2 cut(s) 618, 1341
TaaI ACNGT 3 cut(s) 17, 1012, 1162
TaiI ACGT 2 cut(s) 59, 92
TaqI TCGA 3 cut(s) 470, 584, 1781
TasI AATT 5 cut(s) 538, 580, 686, 936, 1587
TatI WGTACW 2 cut(s) 3, 1103
TauI GCSGC 6 cut(s) 68, 266, 317, 320, 1074, 1363
TfiI GAWTC 5 cut(s) 187, 586, 608, 695, 1694
Tru1I TTAA 3 cut(s) 9, 561, 747
Tru9I TTAA 3 cut(s) 9, 561, 747
TscAI CASTG 4 cut(s) 909, 1408, 1511, 1567
TseFI GTSAC 3 cut(s) 226, 473, 596
TseI GCWGC 4 cut(s) 24, 160, 193, 806
Tsp45I GTSAC 3 cut(s) 226, 473, 596
TspGWI ACGGA 1 cut(s) 1830
TspMI CCCGGG 1 cut(s) 281
TspRI CASTG 4 cut(s) 909, 1408, 1511, 1567
Tth111I GACNNNGTC 1 cut(s) 91
VpaK11BI GGWCC 3 cut(s) 277, 435, 1660
XagI CCTNNNNNAGG 1 cut(s) 122
XapI RAATTY 1 cut(s) 1587
XceI RCATGY 2 cut(s) 1471, 1583
XcmI CCANNNNNNNNNTGG 1 cut(s) 942
XmaI CCCGGG 1 cut(s) 281
XmnI GAANNNNTTC 1 cut(s) 1554
XspI CTAG 7 cut(s) 233, 431, 1496, 1530, 1650, 1751, 1758
ZraI GACGTC 1 cut(s) 90
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.