Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0010740

Physical Location & Seq

Genomic Coordinates Forward (+)

12442155 .. 12449772

Gene Structure 7618 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1127900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2946 bp

ATGGCGAAAGGTACGGATGCTGAGGAGTTCGTGCTGATGTCTAAGGTCCGGGCGGGGCTGAAACGCGAGTTCGCGTTTGCGTTGAAGGCCCAGGCTGAAATCTCTGGCTCTTTGGGGCGGACTCGGGGCAGTAGGGCCCAGAACGGCGACTGTAAGAGGATGAAGACGGAGGAGCGCAGCCGTGGTGATGGTGACGAGAAATTGGGGGGTGGGGAGATTGGGGAGGGCGAGAATGTGAACGTTAAGGAGGAATTGGAGGAGGCTATGAGCGAAGAGGATGCGAAAAGCGATGTGGTGGACGTCATGAGCGACGATGAGCCAAAATTCCATGGGGGCGAGTCTGTGCCCAGCGAGAGGGTTGGGGAAGACGAGTTGAAGCACGGTGTGGTGGAGATGGCAGTAGATGACGAGCCCCAGACTGGTTGCATTGGCGATAGTCAACCAGAAAAGCATTTGGTGGATGAAAAGGAGGAAGGGGAGGTGAGTGAGAAGCCTCCAAGGAGGTTTACTCGGTCAGCGTTGAAGCTTGAGGCAGAAAAAATGCAGAATATACCTGCGGTAGGTTGTACCAAGGAGATGGCAGTAGATGACGAGCCCCAGACCGGTTGCATCGGCGATAGTGAACCCGAAAAGCCTATGGTGGACGAAGAAGTTCCTGTTTTAGTTGAGAACAGAAATGTTAAGGTGGAAGAGGAGGTGATTGAGAAGCCTCCGAGGAGGTTTACTCGGTCAGCATTGAAGCTTGACGCAGAAAAAATGCAGAATATACCTGCCGAAGGTTGTACCAAACAGATAGATTCGGGGATACAGAAAAGCCCTTTTGTTACTCCAATGAAACTTGATACAAAAATGCCGAGTATGGTGAGGAAGTTCAGTAAACTGAAAGAACTTCTTGACACCGGTATTCTTGAGGGGCAGCCAGTCAAGTACCTTAGAGGATCAAAGGTAAGAGAGTCTGGAGAAACAGGGCTTAGGGGAGTGATTAGGGGCTCTTCTATATTGTGTCACTGTGATTCTTGCAAAGGAACAGAAGTTATTACACCTCCTGTGTTTGAGCTGCATGCGGGTAGTTCAATTAAACTTCCTTCGGACCATATTTATCTTGAGAACGGGAAGACCCTTCGGGATGTCATGACCATATGCGAAAATTCTCCCTTGGAAACTTTGGAAGAAGCTGTCCGACTTGTTATTGGTTGCTCATCTATAAGCAAATGTATGATCTGTCTCAATTGCAAAGATTCTATTGACAAAGACCGGACAAGGTCAGCAGTGCTACTTTGTAGCTCATGCATGGAGTTAAAAGAGTCTGGAGCTAGGCCTGCTGTAGGTGCTAAACAGAGTGACGAATCATCAAAGCTGGTTTCAGGTCCAAATAGTCCTGATACCTTGTCAAAGTGTAGCTCACCAGAGCAGGTTACAGTTCCAAAGCATCCATATATGCCAAAGGGTAGCTCATCAAAGCCAGTTAAAGTTCAAAAGCATCCTCATGACGTGCCAAAGTGCCGCTCGTCAGAAAGTAAAAGTCAGGGAAGAGTAACCAAAAAGGATCTTCGGCTGCACAAGTTGGTTTTTGAGGAAGATTGTTTGCTGGATGGCACAGAAGTAGGCTATTTTTCCAGGGGAAAGAAAATGTTGGTTGGTTATAAAAAGGGTCCTGGCATTGTTTGTGGTTGCTGCAGTGATGAGGTCAGCGCCTCGACATTTGAAGCTCATGCTGGTTTTAAGTCACGTCGCAAGCCTTACATGAACATTTACACATCTAATGGGGTATCTCTCCATGAATTGGCATTATCTCTCTCTAGTCGTCGGAAGTGGTCTACCAAGCATAATGATGACCTATGCTCCATCTGTGAAGCTGGTGGAGATCTCTTATGCTGTGATAATTGTCCAAGGGCTTTTCACAAAGAGTGCCTTTCTCTAACAAGTGTCCCTGGCGATACTTGGTACTGTAAGAATTGCCAGACTATGTTTGAGAAAGAAAAATATGTGGAGCATAATCCCAATGCTGTTGCAGCTGGAAGGGTTGCAGGGGTCGATCCCATAGAACAAATAACCAACAGATGCATTCGTATTGTCACTACTTTCGATGAGAAACTTGGTGGATGTGCACTGTGCAGTAGCCATGAGTTCAGCGGCTTGGATTTTGGTCCTGGCACCGTTATTTTGTGTGATCAGTGTGAAAAGGAGTATCATGTAGGTTGTTTGAAAGATAAAGGAATTGAAGATATGAAGGAAATACCGAAAGACAAGTGGTTCTGTTGCTCAGATTGCTTCAGGGTTCATTCAGCTTTGCAGAAGTTGGTAGCTCATGGGGAACAGAAGCTTCCAGACTCCCTTCTGAATGTTGTAAGGAAGAAGCACAATAAGAAAGGTCCGGAGAGTGGAGCTAATCTTGATATAAAATGGAGGGTGCTTAATGGGAAAATGTCTACTGATGATGAAAGTGTGCAGTTGCTTTCCAAGGCTCTTGAAATCTTCCATGATCGTTTTTCCCCAATAATTGATCCTACTTCACGCCAGGACTTTATCAAAGAAATGCTTTATGGAAGGACCATCCAGACCCAAGATTTCGGTGGGATGTACTGTGGAATAATTACAGTAAACCAATTGGTCGTGTCAGCTGGAATGTTTCGTATCTATGGAGCCGAAGTGGCAGAGCTTCCTTTAGTGGCAACATGTGCTGATTTGGAAGGACAGGGTTACTTCCAGATCTTGTTTTCATGTATTGAGAGGCTTCTTGCATTTCTAAACGTGAAAAGCCTTGTGCTGCCAGCCGCTAGCGAGGCAGAATCAATATGGAAAAATAGATTTGGTTTTGAGAAGTTAACCCAAAATGAGATACATGATTACAGGAAAAGTTACCAAATGATGATCTTCCAAGGGACTAACATGCTGCGGAAGCCCGTCCCCAAGTGCCGAATCCTTAGGTCACATATAAGCAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000118 GO:0000122 GO:0000228 GO:0000785 GO:0000790 GO:0001085 GO:0001103 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005813 GO:0005815 GO:0005856 GO:0006355 GO:0006357 GO:0008134 GO:0008150 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0015630 GO:0016043 GO:0016581 GO:0017053 GO:0019219 GO:0019222 GO:0019899 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032991 GO:0032993 GO:0042826 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0045892 GO:0045934 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050808 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0060255 GO:0065007 GO:0070013 GO:0070491 GO:0070603 GO:0071840 GO:0072553 GO:0080090 GO:0090545 GO:0090568 GO:0099172 GO:1902494 GO:1902679 GO:1903506 GO:1903507 GO:1904949 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Jas PHD

Protein Analysis

982

Amino Acids

108.65

Weight (kDa)

6.11

Isoelectric Point (pI)

47.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TDBD	PF16135	326 - 379	4.4e-12	Tify domain binding domain
TDBD	PF16135	550 - 595	4.8e-13	Tify domain binding domain
PHD-1st_NSD	PF23011	610 - 654	1e-08	Histone-lysine N-methyltransferase NSD-like, PHD zinc finger 1
PHD	PF00628	614 - 653	5.3e-07	PHD-finger
IDM1_C	PF23209	830 - 968	2e-51	Increased DNA methylation 1, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0010740)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G27980
fragaria_vesca	FvH4_3g30510 FvH4_3g30510 FvH4_3g30510
malus_domestica	MD03G1127900.v1.1 MD11G1149500.v1.1
prunus_persica	Prupe.6G113900_v2.0.a1 Prupe.6G113900_v2.0.a1 Prupe.6G113900_v2.0.a1
pyrus_communis	pycom11g12240
rosa_chinensis	RchiOBHm_Chr5g0056471
rosa_laevigata	RLG00000035101
rosa_multiflora	Rmu_sc0002761.1_g000015
rosa_roxburghii	Rroxscaffold_1G00023850
rosa_rugosa	Rorug05G0300200
rosa_samantha	Rh5AG370700 Rh5BG382000 Rh5CG405100 Rh5DG394900
rosa_wichuraiana	Rw5G034720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1644
AatII	GACGTC	1 cut(s)	303
Acc36I	ACCTGC	3 cut(s)	562, 778, 1402
AccB1I	GGYRCC	1 cut(s)	2153
AccB7I	CCANNNNNTGG	1 cut(s)	1783
AccBSI	CCGCTC	1 cut(s)	1506
AccI	GTMKAC	2 cut(s)	1817, 2429
AccII	CGCG	2 cut(s)	66, 74
AccIII	TCCGGA	1 cut(s)	2374
AciI	CCGC	8 cut(s)	53, 118, 557, 1064, 1504, 2133, 2775, 2896
AclI	AACGTT	1 cut(s)	240
AclWI	GGATC	4 cut(s)	946, 1554, 2030, 2498
AcsI	RAATTY	2 cut(s)	323, 1147
AcuI	CTGAAG	1 cut(s)	2257
AcyI	GRCGYC	1 cut(s)	300
AdeI	CACNNNGTG	1 cut(s)	385
AfaI	GTAC	6 cut(s)	13, 568, 784, 929, 1946, 2582
AfiI	CCNNNNNNNGG	8 cut(s)	354, 419, 560, 602, 776, 1325, 1783, 2381
AflIII	ACRYGT	1 cut(s)	2675
AgeI	ACCGGT	2 cut(s)	602, 899
AjiI	CACGTC	2 cut(s)	1492, 1730
AjnI	CCWGG	6 cut(s)	90, 1616, 1654, 1930, 2149, 2517
Alw21I	GWGCWC	1 cut(s)	2110
Alw26I	GTCTC	1 cut(s)	1229
Alw44I	GTGCAC	1 cut(s)	2106
AlwI	GGATC	4 cut(s)	946, 1554, 2030, 2498
Ama87I	CYCGRG	1 cut(s)	123
Aor13HI	TCCGGA	1 cut(s)	2374
AoxI	GGCC	3 cut(s)	87, 135, 1316
ApaI	GGGCCC	1 cut(s)	139
ApaLI	GTGCAC	1 cut(s)	2106
ApeKI	GCWGC	8 cut(s)	177, 916, 1057, 1555, 1674, 2012, 2767, 2893
ApoI	RAATTY	2 cut(s)	323, 1147
AsiGI	ACCGGT	2 cut(s)	602, 899
Asp700I	GAANNNNTTC	1 cut(s)	651
AspLEI	GCGC	2 cut(s)	177, 1694
AsuC2I	CCSGG	1 cut(s)	50
AsuHPI	GGTGA	6 cut(s)	197, 203, 493, 709, 874, 1395
AsuNHI	GCTAGC	1 cut(s)	2777
AvaI	CYCGRG	1 cut(s)	123
AvaII	GGWCC	7 cut(s)	46, 1090, 1367, 1652, 2147, 2372, 2550
AxyI	CCTNAGG	1 cut(s)	2924
BaeGI	GKGCMC	3 cut(s)	139, 348, 2110
BanI	GGYRCC	1 cut(s)	2153
BanII	GRGCYC	4 cut(s)	139, 414, 597, 992
BbsI	GAAGAC	3 cut(s)	170, 372, 1121
Bbv12I	GWGCWC	1 cut(s)	2110
BbvCI	CCTCAGC	1 cut(s)	21
BbvI	GCAGC	8 cut(s)	189, 928, 1044, 1542, 1661, 2024, 2754, 2880
BccI	CCATC	6 cut(s)	182, 388, 571, 1586, 1853, 2561
BceAI	ACGGC	2 cut(s)	160, 165
BcgI	CGANNNNNNTGC	2 cut(s)	260, 294
BciT130I	CCWGG	6 cut(s)	92, 1618, 1656, 1932, 2151, 2519
BciVI	GTATCC	1 cut(s)	798
BclI	TGATCA	1 cut(s)	2170
BcnI	CCSGG	1 cut(s)	50
BcoDI	GTCTC	1 cut(s)	1229
BfaI	CTAG	3 cut(s)	1314, 1800, 2778
BfmI	CTRYAG	2 cut(s)	1323, 1675
BfoI	RGCGCY	1 cut(s)	1695
BfuAI	ACCTGC	3 cut(s)	562, 778, 1402
BfuI	GTATCC	1 cut(s)	798
BglI	GCCNNNNNGGC	1 cut(s)	2651
BglII	AGATCT	2 cut(s)	1864, 2709
Bme1390I	CCNGG	7 cut(s)	50, 92, 1618, 1656, 1932, 2151, 2519
Bme18I	GGWCC	7 cut(s)	46, 1090, 1367, 1652, 2147, 2372, 2550
BmeT110I	CYCGRG	1 cut(s)	123
BmgBI	CACGTC	2 cut(s)	1492, 1730
BmiI	GGNNCC	4 cut(s)	137, 1653, 2155, 2644
BmrFI	CCNGG	7 cut(s)	50, 92, 1618, 1656, 1932, 2151, 2519
BmsI	GCATC	6 cut(s)	7, 268, 618, 1438, 1489, 2051
BmtI	GCTAGC	1 cut(s)	2781
BpiI	GAAGAC	3 cut(s)	170, 372, 1121
BplI	GAGNNNNNCTC	4 cut(s)	493, 525, 709, 741
BpmI	CTGGAG	2 cut(s)	978, 1329
Bpu10I	CCTNAGC	2 cut(s)	21, 971
BpuEI	CTTGAG	3 cut(s)	548, 929, 1124
BpuMI	CCSGG	1 cut(s)	50
BsaHI	GRCGYC	1 cut(s)	300
BsaWI	WCCGGW	4 cut(s)	602, 899, 1254, 2374
BsaXI	ACNNNNNCTCC	4 cut(s)	1826, 1856, 2634, 2664
Bsc4I	CCNNNNNNNGG	8 cut(s)	354, 419, 560, 602, 776, 1325, 1783, 2381
Bse118I	RCCGGY	2 cut(s)	602, 899
Bse1I	ACTGG	3 cut(s)	424, 920, 1463
Bse21I	CCTNAGG	1 cut(s)	2924
BseAI	TCCGGA	1 cut(s)	2374
BseBI	CCWGG	6 cut(s)	92, 1618, 1656, 1932, 2151, 2519
BseLI	CCNNNNNNNGG	8 cut(s)	354, 419, 560, 602, 776, 1325, 1783, 2381
BseMII	CTCAG	2 cut(s)	12, 2277
BseNI	ACTGG	3 cut(s)	424, 920, 1463
BseRI	GAGGAG	5 cut(s)	38, 185, 272, 707, 730
BseSI	GKGCMC	3 cut(s)	139, 348, 2110
BseXI	GCAGC	8 cut(s)	189, 928, 1044, 1542, 1661, 2024, 2754, 2880
BseYI	CCCAGC	1 cut(s)	347
BsgI	GTGCAG	3 cut(s)	1541, 2134, 2468
Bsh1236I	CGCG	2 cut(s)	66, 74
BshFI	GGCC	3 cut(s)	89, 137, 1318
BshNI	GGYRCC	1 cut(s)	2153
BshTI	ACCGGT	2 cut(s)	602, 899
BsiHKAI	GWGCWC	1 cut(s)	2110
BsiHKCI	CYCGRG	1 cut(s)	123
BsiSI	CCGG	5 cut(s)	49, 603, 900, 1255, 2375
BslFI	GGGAC	3 cut(s)	1913, 2891, 2896
BslI	CCNNNNNNNGG	8 cut(s)	354, 419, 560, 602, 776, 1325, 1783, 2381
BsmAI	GTCTC	1 cut(s)	1229
BsmFI	GGGAC	3 cut(s)	1913, 2891, 2896
BsmI	GAATGC	1 cut(s)	2064
BsnI	GGCC	3 cut(s)	89, 137, 1318
BsoBI	CYCGRG	1 cut(s)	123
Bsp120I	GGGCCC	1 cut(s)	135
Bsp1286I	GDGCHC	6 cut(s)	139, 348, 414, 597, 992, 2110
Bsp13I	TCCGGA	1 cut(s)	2374
Bsp19I	CCATGG	1 cut(s)	328
BspACI	CCGC	8 cut(s)	53, 118, 557, 1064, 1504, 2133, 2775, 2896
BspANI	GGCC	3 cut(s)	89, 137, 1318
BspCNI	CTCAG	2 cut(s)	13, 2276
BspEI	TCCGGA	1 cut(s)	2374
BspFNI	CGCG	2 cut(s)	66, 74
BspHI	TCATGA	3 cut(s)	303, 1131, 1486
BspLI	GGNNCC	4 cut(s)	137, 1653, 2155, 2644
BspMAI	CTGCAG	1 cut(s)	1679
BspMI	ACCTGC	3 cut(s)	562, 778, 1402
BspOI	GCTAGC	1 cut(s)	2781
BspPI	GGATC	4 cut(s)	946, 1554, 2030, 2498
BspQI	GCTCTTC	1 cut(s)	997
BspT107I	GGYRCC	1 cut(s)	2153
BsrBI	CCGCTC	1 cut(s)	1506
BsrFI	RCCGGY	2 cut(s)	602, 899
BsrI	ACTGG	3 cut(s)	424, 920, 1463
BssAI	RCCGGY	2 cut(s)	602, 899
BssNI	GRCGYC	1 cut(s)	300
BssT1I	CCWWGG	7 cut(s)	328, 497, 570, 1155, 1889, 2460, 2878
Bst2UI	CCWGG	6 cut(s)	92, 1618, 1656, 1932, 2151, 2519
Bst4CI	ACNGT	9 cut(s)	152, 383, 1010, 1420, 1949, 2112, 2158, 2585, 2599
Bst6I	CTCTTC	4 cut(s)	267, 684, 997, 1525
BstACI	GRCGYC	1 cut(s)	300
BstAPI	GCANNNNNTGC	1 cut(s)	2678
BstC8I	GCNNGC	5 cut(s)	1062, 1320, 1736, 2772, 2779
BstDEI	CTNAG	6 cut(s)	21, 42, 932, 971, 2263, 2924
BstDSI	CCRYGG	2 cut(s)	181, 328
BstENI	CCTNNNNNAGG	3 cut(s)	558, 774, 1323
BstFNI	CGCG	2 cut(s)	66, 74
BstH2I	RGCGCY	1 cut(s)	1695
BstHHI	GCGC	2 cut(s)	177, 1694
BstMAI	GTCTC	1 cut(s)	1229
BstMWI	GCNNNNNNNGC	9 cut(s)	86, 1319, 1328, 2012, 2268, 2651, 2678, 2783, 2899
BstNI	CCWGG	6 cut(s)	92, 1618, 1656, 1932, 2151, 2519
BstNSI	RCATGY	3 cut(s)	1064, 2679, 2893
BstSCI	CCNGG	7 cut(s)	48, 90, 1616, 1654, 1930, 2149, 2517
BstSFI	CTRYAG	2 cut(s)	1323, 1675
BstSLI	GKGCMC	3 cut(s)	139, 348, 2110
BstUI	CGCG	2 cut(s)	66, 74
BstV1I	GCAGC	8 cut(s)	189, 928, 1044, 1542, 1661, 2024, 2754, 2880
BstV2I	GAAGAC	3 cut(s)	170, 372, 1121
BstX2I	RGATCY	3 cut(s)	1546, 1864, 2709
BstXI	CCANNNNNNTGG	1 cut(s)	577
BstYI	RGATCY	3 cut(s)	1546, 1864, 2709
Bsu36I	CCTNAGG	1 cut(s)	2924
BsuI	GTATCC	1 cut(s)	798
BsuRI	GGCC	3 cut(s)	89, 137, 1318
BtgI	CCRYGG	2 cut(s)	181, 328
BtgZI	GCGATG	1 cut(s)	303
BtrI	CACGTC	2 cut(s)	1492, 1730
BtsI	GCAGTG	2 cut(s)	1275, 1684
BtsIMutI	CAGTG	5 cut(s)	1006, 1275, 1684, 2108, 2180
BveI	ACCTGC	3 cut(s)	562, 778, 1402
Cac8I	GCNNGC	5 cut(s)	1062, 1320, 1736, 2772, 2779
CciI	TCATGA	3 cut(s)	303, 1131, 1486
CfoI	GCGC	2 cut(s)	177, 1694
Cfr10I	RCCGGY	2 cut(s)	602, 899
CseI	GACGC	1 cut(s)	755
Csp6I	GTAC	6 cut(s)	12, 567, 783, 928, 1945, 2581
CspAI	ACCGGT	2 cut(s)	602, 899
CviQI	GTAC	6 cut(s)	12, 567, 783, 928, 1945, 2581
DdeI	CTNAG	6 cut(s)	21, 42, 932, 971, 2263, 2924
DraIII	CACNNNGTG	1 cut(s)	385
Eam1104I	CTCTTC	4 cut(s)	267, 684, 997, 1525
EarI	CTCTTC	4 cut(s)	267, 684, 997, 1525
EciI	GGCGGA	1 cut(s)	133
Eco130I	CCWWGG	7 cut(s)	328, 497, 570, 1155, 1889, 2460, 2878
Eco147I	AGGCCT	1 cut(s)	1318
Eco24I	GRGCYC	4 cut(s)	139, 414, 597, 992
Eco47I	GGWCC	7 cut(s)	46, 1090, 1367, 1652, 2147, 2372, 2550
Eco57I	CTGAAG	1 cut(s)	2257
Eco81I	CCTNAGG	1 cut(s)	2924
Eco88I	CYCGRG	1 cut(s)	123
EcoNI	CCTNNNNNAGG	3 cut(s)	558, 774, 1323
EcoO109I	RGGNCCY	2 cut(s)	135, 1652
EcoRII	CCWGG	6 cut(s)	90, 1616, 1654, 1930, 2149, 2517
EcoT14I	CCWWGG	7 cut(s)	328, 497, 570, 1155, 1889, 2460, 2878
EcoT22I	ATGCAT	2 cut(s)	1292, 2066
EcoT38I	GRGCYC	4 cut(s)	139, 414, 597, 992
ErhI	CCWWGG	7 cut(s)	328, 497, 570, 1155, 1889, 2460, 2878
FalI	AAGNNNNNCTT	6 cut(s)	876, 908, 1896, 1928, 2523, 2555
FaqI	GGGAC	3 cut(s)	1913, 2891, 2896
FauI	CCCGC	2 cut(s)	46, 1057
FauNDI	CATATG	1 cut(s)	1139
FbaI	TGATCA	1 cut(s)	2170
FblI	GTMKAC	2 cut(s)	1817, 2429
FriOI	GRGCYC	4 cut(s)	139, 414, 597, 992
FspBI	CTAG	3 cut(s)	1314, 1800, 2778
GlaI	GCGC	2 cut(s)	176, 1693
GsaI	CCCAGC	1 cut(s)	351
GsuI	CTGGAG	2 cut(s)	978, 1329
HaeII	RGCGCY	1 cut(s)	1695
HaeIII	GGCC	3 cut(s)	89, 137, 1318
HapII	CCGG	5 cut(s)	49, 603, 900, 1255, 2375
HgaI	GACGC	1 cut(s)	755
HhaI	GCGC	2 cut(s)	177, 1694
Hin1I	GRCGYC	1 cut(s)	300
Hin6I	GCGC	2 cut(s)	175, 1692
HinP1I	GCGC	2 cut(s)	175, 1692
HincII	GTYRAC	2 cut(s)	440, 2826
HindII	GTYRAC	2 cut(s)	440, 2826
HindIII	AAGCTT	3 cut(s)	524, 740, 2321
HpaI	GTTAAC	1 cut(s)	2826
HpaII	CCGG	5 cut(s)	49, 603, 900, 1255, 2375
HphI	GGTGA	6 cut(s)	197, 203, 493, 709, 874, 1395
Hpy188I	TCNGA	7 cut(s)	714, 1090, 1181, 1513, 1809, 2266, 2340
Hpy99I	CGWCG	3 cut(s)	314, 1734, 1809
HpyCH4III	ACNGT	9 cut(s)	152, 383, 1010, 1420, 1949, 2112, 2158, 2585, 2599
HpyCH4IV	ACGT	5 cut(s)	240, 300, 1491, 1729, 2751
HpyF10VI	GCNNNNNNNGC	9 cut(s)	86, 1319, 1328, 2012, 2268, 2651, 2678, 2783, 2899
HpyF3I	CTNAG	6 cut(s)	21, 42, 932, 971, 2263, 2924
HpySE526I	ACGT	5 cut(s)	240, 300, 1491, 1729, 2751
Hsp92I	GRCGYC	1 cut(s)	300
HspAI	GCGC	2 cut(s)	175, 1692
Kpn2I	TCCGGA	1 cut(s)	2374
Ksp22I	TGATCA	1 cut(s)	2170
KspAI	GTTAAC	1 cut(s)	2826
LguI	GCTCTTC	1 cut(s)	997
LmnI	GCTCC	6 cut(s)	172, 1310, 1847, 1990, 2384, 2642
Lsp1109I	GCAGC	8 cut(s)	189, 928, 1044, 1542, 1661, 2024, 2754, 2880
LweI	GCATC	6 cut(s)	7, 268, 618, 1438, 1489, 2051
MaeI	CTAG	3 cut(s)	1314, 1800, 2778
MaeII	ACGT	5 cut(s)	240, 300, 1491, 1729, 2751
MbiI	CCGCTC	1 cut(s)	1506
MfeI	CAATTG	2 cut(s)	1228, 2606
MflI	RGATCY	3 cut(s)	1546, 1864, 2709
MhlI	GDGCHC	6 cut(s)	139, 348, 414, 597, 992, 2110
MlyI	GAGTC	5 cut(s)	115, 347, 962, 1313, 2324
MmeI	TCCRAC	2 cut(s)	1204, 1787
Mph1103I	ATGCAT	2 cut(s)	1292, 2066
MroI	TCCGGA	1 cut(s)	2374
MroXI	GAANNNNTTC	1 cut(s)	651
MseI	TTAA	8 cut(s)	243, 681, 1077, 1298, 1467, 1722, 2415, 2825
MslI	CAYNNNNRTG	2 cut(s)	1485, 1830
MspA1I	CMGCKG	3 cut(s)	2015, 2133, 2621
MspI	CCGG	5 cut(s)	49, 603, 900, 1255, 2375
MspR9I	CCNGG	7 cut(s)	50, 92, 1618, 1656, 1932, 2151, 2519
MunI	CAATTG	2 cut(s)	1228, 2606
Mva1269I	GAATGC	1 cut(s)	2064
MvaI	CCWGG	6 cut(s)	92, 1618, 1656, 1932, 2151, 2519
MvnI	CGCG	2 cut(s)	66, 74
MwoI	GCNNNNNNNGC	9 cut(s)	86, 1319, 1328, 2012, 2268, 2651, 2678, 2783, 2899
NciI	CCSGG	1 cut(s)	50
NcoI	CCATGG	1 cut(s)	328
NdeI	CATATG	1 cut(s)	1139
NheI	GCTAGC	1 cut(s)	2777
NlaIV	GGNNCC	4 cut(s)	137, 1653, 2155, 2644
NmeAIII	GCCGAG	1 cut(s)	879
NmuCI	GTSAC	6 cut(s)	191, 1004, 1340, 1725, 2074, 2928
NsiI	ATGCAT	2 cut(s)	1292, 2066
NspI	RCATGY	3 cut(s)	1064, 2679, 2893
PaeI	GCATGC	1 cut(s)	1064
PagI	TCATGA	3 cut(s)	303, 1131, 1486
PceI	AGGCCT	1 cut(s)	1318
PciI	ACATGT	1 cut(s)	2675
PciSI	GCTCTTC	1 cut(s)	997
PcsI	WCGNNNNNNNCGW	1 cut(s)	306
PctI	GAATGC	1 cut(s)	2064
PdmI	GAANNNNTTC	1 cut(s)	651
PfeI	GAWTC	6 cut(s)	797, 1013, 1238, 1346, 2789, 2919
PflFI	GACNNNGTC	1 cut(s)	1261
PflMI	CCANNNNNTGG	1 cut(s)	1783
PinAI	ACCGGT	2 cut(s)	602, 899
PleI	GAGTC	5 cut(s)	115, 346, 961, 1312, 2324
PpsI	GAGTC	5 cut(s)	115, 346, 961, 1312, 2324
PpuMI	RGGWCCY	1 cut(s)	1652
PscI	ACATGT	1 cut(s)	2675
PsiI	TTATAA	1 cut(s)	1644
Psp1406I	AACGTT	1 cut(s)	240
Psp5II	RGGWCCY	1 cut(s)	1652
Psp6I	CCWGG	6 cut(s)	90, 1616, 1654, 1930, 2149, 2517
PspFI	CCCAGC	1 cut(s)	347
PspGI	CCWGG	6 cut(s)	90, 1616, 1654, 1930, 2149, 2517
PspN4I	GGNNCC	4 cut(s)	137, 1653, 2155, 2644
PspOMI	GGGCCC	1 cut(s)	135
PspPPI	RGGWCCY	1 cut(s)	1652
PstI	CTGCAG	1 cut(s)	1679
PsuI	RGATCY	3 cut(s)	1546, 1864, 2709
PsyI	GACNNNGTC	1 cut(s)	1261
PvuII	CAGCTG	2 cut(s)	2015, 2621
RsaI	GTAC	6 cut(s)	13, 568, 784, 929, 1946, 2582
RsaNI	GTAC	6 cut(s)	12, 567, 783, 928, 1945, 2581
RseI	CAYNNNNRTG	2 cut(s)	1485, 1830
SapI	GCTCTTC	1 cut(s)	997
SaqAI	TTAA	8 cut(s)	243, 681, 1077, 1298, 1467, 1722, 2415, 2825
SchI	GAGTC	5 cut(s)	115, 347, 962, 1313, 2324
ScrFI	CCNGG	7 cut(s)	50, 92, 1618, 1656, 1932, 2151, 2519
SduI	GDGCHC	6 cut(s)	139, 348, 414, 597, 992, 2110
SfaNI	GCATC	6 cut(s)	7, 268, 618, 1438, 1489, 2051
SfcI	CTRYAG	2 cut(s)	1323, 1675
SinI	GGWCC	7 cut(s)	46, 1090, 1367, 1652, 2147, 2372, 2550
SmiMI	CAYNNNNRTG	2 cut(s)	1485, 1830
SmlI	CTYRAG	3 cut(s)	527, 908, 1103
SmoI	CTYRAG	3 cut(s)	527, 908, 1103
SphI	GCATGC	1 cut(s)	1064
SseBI	AGGCCT	1 cut(s)	1318
SsiI	CCGC	8 cut(s)	53, 118, 557, 1064, 1504, 2133, 2775, 2896
SspMI	CTAG	3 cut(s)	1314, 1800, 2778
StuI	AGGCCT	1 cut(s)	1318
StyD4I	CCNGG	7 cut(s)	48, 90, 1616, 1654, 1930, 2149, 2517
StyI	CCWWGG	7 cut(s)	328, 497, 570, 1155, 1889, 2460, 2878
TaaI	ACNGT	9 cut(s)	152, 383, 1010, 1420, 1949, 2112, 2158, 2585, 2599
TaiI	ACGT	5 cut(s)	243, 303, 1494, 1732, 2754
TaqI	TCGA	3 cut(s)	1697, 2034, 2085
TaqII	GACCGA	2 cut(s)	501, 717
TatI	WGTACW	1 cut(s)	2580
TauI	GCSGC	3 cut(s)	1506, 2136, 2777
TfiI	GAWTC	6 cut(s)	797, 1013, 1238, 1346, 2789, 2919
Tru1I	TTAA	8 cut(s)	243, 681, 1077, 1298, 1467, 1722, 2415, 2825
Tru9I	TTAA	8 cut(s)	243, 681, 1077, 1298, 1467, 1722, 2415, 2825
TscAI	CASTG	5 cut(s)	1013, 1275, 1684, 2115, 2180
TseFI	GTSAC	6 cut(s)	191, 1004, 1340, 1725, 2074, 2928
TseI	GCWGC	8 cut(s)	177, 916, 1057, 1555, 1674, 2012, 2767, 2893
Tsp45I	GTSAC	6 cut(s)	191, 1004, 1340, 1725, 2074, 2928
TspDTI	ATGAA	9 cut(s)	176, 477, 848, 1760, 1794, 2243, 2270, 2454, 2709
TspGWI	ACGGA	2 cut(s)	29, 182
TspRI	CASTG	5 cut(s)	1013, 1275, 1684, 2115, 2180
Tth111I	GACNNNGTC	1 cut(s)	1261
Van91I	CCANNNNNTGG	1 cut(s)	1783
VneI	GTGCAC	1 cut(s)	2106
VpaK11BI	GGWCC	7 cut(s)	46, 1090, 1367, 1652, 2147, 2372, 2550
XagI	CCTNNNNNAGG	3 cut(s)	558, 774, 1323
XapI	RAATTY	2 cut(s)	323, 1147
XceI	RCATGY	3 cut(s)	1064, 2679, 2893
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2570
XmiI	GTMKAC	2 cut(s)	1817, 2429
XmnI	GAANNNNTTC	1 cut(s)	651
XspI	CTAG	3 cut(s)	1314, 1800, 2778
ZraI	GACGTC	1 cut(s)	301
Zsp2I	ATGCAT	2 cut(s)	1292, 2066

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.