MD03G1132800.v1.1

WSTF, HB1, Itc1p, MBD9 motif 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0007748
Physical Location & Seq
Reverse (-)
13206339 .. 13213938
7600 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1132800.v1.1.491

Sequence Viewer

Length: 4998 bp
ATGGACAATCCGGTGGTCAAAAAGAGGGGTCGCCCTAGGAAGCGTAAAGCGGAGGATGAAAAGGACCTGAAATTTGGTCCGGAAGCTAAGAAACGGGCTGTGGAGACAAGGTTGATTGTGTTGGTGGGTAGATATGTGTTGAAAGACTTCGGAAGGAGTGGGATTTTTCTTGGGAAAGTTGTGTATTATGGTGCCGGGTTGTATAGAGTTAATTACGAAGATGGCGATTGTGAGGACTTGGAGAGTCGCGAGATTCGTGGCATCCTTGTTGGTGATGATGATTTTGACACTGGTTTGAATGTTAGGAGGAAGAAATTAGATGATTTAGTGTCGAAATTGAATAGGGAAACTGCAGCTGGGTTGGATAAGAATGTTGTAAACTCAGTCCAGGAGGTGGATAGGATCGACTCACCAGCATTGAGTGATTTGGGCGGTGGAGTAAAGATCGAGAGTGATGAGACTCAAATTGAAGGTGACGATGATTCGTCTAGTGATTCAAGTGAGTATGCACGGGATAGAGATATGGGTTTTGATGATAAGCCACCCCCTGTCCCACCACCACAATTGCCGCCTTCTTCAGGTACCATAGGCGTGCCAGAGCAGTATGTTTCACATCTTTTCTCCGTATATGGTTTCTTGCGATCACTTAGCATCTCCCTGTTCTTGAACCCCTTCACACTGGATGATTTTGTGGGATCGCTTAATTGCTGTGCTCCAAACACACTGTTGGATGCAATTCATGTTGTTTTGCTGCGTGCTTTGAGGCGTCATCTTGAAACACTCTCATCTGATGGCTCAGAGGTAGCACCAAAGTGTTTGAGGTGCATTGATTGGAACTTGATTGATACATTGACTTGGCCAGTTTACTTGGTCCAGTATTTGACAATTATGGGATATACAAAAGGACCTGATTGGAGAGGCTTTTATAATGATGTTTTGGGCAAGGAGTATTATCTACTATCAGTTGGTAGAAAGTTAATTATTTTGCAAATCCTTTGCGACGATGTCTTAGATGCTAAAGATCTGAGAGATGAATTAGACATGCGTGAAGAATCAGAAGTCGGAATAGATTATGATGCAGAAGTAACGAATCCTCTTGTAAGTGGGCCAAGAAGGGTCCACCCGAGATATTCTAAAACTTCCGCCTGGAAGGACAGAGAAGAGGTGGAAACTAATCACACATCTGGTAATGCTAATTCAATCAGTTCCAAAGGTGTAAAAGAGGACATGGATGATGTAGATCACAACTCTGATGAATGTCGGCTTTGTGGCATGGATGGAACCTTGCTTTGCTGTGATGGGTGTCCTTCTGCTTACCACACAAGGTGTATTGGTGTTATGAAATTGTCTATACCAGAAGGGTCCTGGTATTGTCCTGAGTGCACAATTGATAAGATTGGGCCAACAATTACAATGGGAACATCTGTTAAAGGAGCACAAATATTTGGCATTGATTCCTACGAGCACATCTTCATGGGTACTTGCAACCACTTACTGGTGCTCAAGGAAACTATCAATACAGAACCATGTGTTAGATATTACAATCAGAATGATATTCCAAAAGTCCTTAAAGTTTTACATTCATCTGGCCAACATACAGCTTTATACTCGGATGTGTGCAAGGCAATTTTACAATATTGGAGTATTCCAGAAAGTATCTTATCTTTTCCAGAAGCAACTGAAACAGAAATTCCATTGGAATATACAAAGGAAGGTGCAAAATCTTCTACTCGGTCACTTCCTTTGCCTGGCAAGGAAAATCATACAGTTACAGATCCGATCAAGGCTGAGAATTATGTGACTAGTGTAAGTGGTACCAATTTAGGTTACACGGCAGCTTCATCTCTCAATGCCTCTGATAAGATCCAAGCTGACCTTCCTAATTTTCAGAGCAATGGTGGCAGTGAACCACAGGAGCGCCGGCCCTTGATTATCAAGATACGTACAAGGCAGAAAATGGAATTTTCTGATCCTAATGGTACTGGTAGCCAGCAGGCTGATCCATCTCATCTGACCCACCAAAGTTCAGTTGAGAGACCGCTTGCAGTAGCCCGTAGTACATGCACCTCAGGGAACATCAACAGTTCTTACAGTGAGCATGCATGCATGGGGGCTTTTTATAAACCTCAGGCATACATAAATTACTATATGCATGGAGAGTTTGCTGCATCTGCTGCTTCCAAACTTGCTGCAATATCATCAGAAGAAACTCGGGTCTCAGATAGTCAGGCTTTAGGCAATCCCAGGAAAGTTGCGTCTGCAAACATTTCATTGCAAACAAAAGCCTTCTCATTAACAGCCTCACGATTCTTCTGGCCAAGTTCTGAAAAGAAGCTTGTGGAGGTTCCAAGGGAGAGGTGTGGTTGGTGTCTTTCTTGTAAGGCTTCAGTGTCAAGCAAGAGAGGATGTATGCTAAATCATGCTGCTTTGAGTGCCACCAAAGGTACTATGAAGACCCTTGCCAGCCTGCATCCAATAAAGAATGGGGAGGGGAATCTTGTAAGCATTGCAACATATGTTTTATACATGGAGGAGAGTTTAGGTGGCCTAGTAACTGGACCCTTTGTAAATGAAAATTATAGAAACCAGTGGCGCAAACAAATTTATCAAGGATCAAGTTTCCGTGCAATAAAAGCGCTATTGCTTGAACTTGAGGCAAATATCCGAACCATTGCTCTATCTGGGGAGTGGATTAAGCTGGTGGATGATTGGTTGGTTGAATCTTTGATGATTCAAAGTGCAACTTGCTCTGTTGGCCCTACACAAAAACGCGGACCAAACAGCAGGAGGGGTAGGAAGCAGAATGCGATTCATGACGATAAAGATGATGATTGCCATGACCAGAGTTTTGTCTGGTGGCAAGGTGGGAAGCTATCAAAGCTTATATTCCAGAGAGCAATTTTATCATTTTCACTGGTTAAAAAAGCAGCTCGCCAGGGTGGTTGGAAGAAAATACCTGGTATATCTTATGCTGATGGTTCTGAGATTCCTAAAAGAAGCAGGCAGTCGGTATGGAGGGCTGCAGTTGAAATGAGTAAGAACGCATCACAGCTTGCGCTTCAGGTCAGATATTTGGACTATCATCTGAGATGGAGTGATCTTGTTCGTCCTGAGCAGAACCTCCCTGATGGGAAGGGTGTGGAGACAGATGCATCTGTTTTTAGAAATGCAAATATATGTGATAAAAAATCTGTGAAGAACAGTACCATATATGGGGTTGATTTTGGTAGTCAAAAGCATCTGCCTAACCGTGTAATGAAGAGTATCATTGAAACAGAGGAAAGTCAAGATGGATATAACAAGTTTTGGTTTCCTGAACTGCGCATTCCTTTATATTTGATTAAAGAATATGAAGAAAGGGTGGGCAAAGCTCTTTTGCCATCAGCTGAGGAGCCTTTGAATATGTTTCGCAAGTTACAGAAACAGCAGTGGAAGGTTCCCCGCAGGGACATATTTTTCTATCTTGTGTGCAAGAGAGAGAATCTGGACTTGTGTTCCTGCTCTTCATGTCAACTGAATGTTTTAATGCGGAATGCATTCAAGTGCAGCATCTGTCAAGGTTATTGTCATGAGGACTGTACTATGAGTTCAACAGTTTCTACAAATGAAGAGGTTGAGTTCTTGATCACTTGCAAGCAGTGCTACCATGCAAAGGCTTTCACTAAAAATGAAAACTTGAAGGAGTCTCCAACTAGTCCTTTGCACTTGCAAATGCAAGAATATCAAACTCCCATGACAGTCACCAGTGTTGCACGGACCAAAAATTACAATCAACCAGTAACACATGTCAAAGGACAAGAGACGCGTTCCGAGAAGAAAGCAACTTCAGAGTCTGCTTTGGCAGCAAAAAGACGTCGCGGCATTTGTTCTTGGGGTATTATATGGAAAAAGAAGAACGGCCAGGAAACTGACACTGATTTCCTGCTTAATAACATACTTCTTGCGGATGGTTCAGAAGTGCATGGGTTGTATCCTGTTTGCCACTTGTGCCATATGCCATATCAGTCAGATCTGATGTATATATGCTGCGAAACTTGCAAAAGTAAGTGTGCTCCCATAAGTGATTGGTACCATGCTGAAGCTGTTGAGCTCAACGAGTCAAAGATTTCTGAAGTGGCTGGTTTCAAGTGTTGCAAGTGTCGGAGGATAAAATCACCTGTGTGTCCTTACACTGATTCTAAAAACATAAAGATGCCTGAGAATAAGAAGGTTCACACTAGGCGTTCAAAGCAAGAAACAGTTGGAGAGGACTCTGATTCTGCAACCATTTCCATATCTGATTCTAAATTGTACGAACCTGCTACTCCAATATTTCCCATGGAGGAAGTGCCCATGCAGGATGGTGATCCTCTTCTCTTTTCTCTCGCTAAAGTTGAGTTAGTTACAGAGTACGATTCAGAAGTTAATGCTCAATGGAATGCTGCTGAGCCAGGGCCTAGGAAATTACCAGTGAGAAGGGGTATTAAGCGTGAAGAGGATGTAGATGGCTTTCATGAGAGTAACATATCTCATCCTGGAATATCTACACATGGTGAAATGAATTACCTGTCAAATTCCATGGACTTTGCACCATCCCATGTTGAATGGGATGCCTCCATGAATGGATTCGAAGGTGAGATGGTGCATGACTATGAAGATCTGAACTATGAGAATATGGAACCGCAAACTGTCTTCACTATCAATGAGTTGCTTGCACCTGATGATGGTGACCAGTTGGATGGATTCGAAGCATTTGCAGATGAATCAGGAAATCTGGAAAACCAATATACAGTTTTCCAGGATGGAGGCCCTGATCAATATAACATAGCTACTTTCACTGATGAAGTAAAGTCTGCTGTTGATATGATGCAATGTCAGGTATGTTCACTTCAAGAACCATCCCCCGATCTTTCCTGCCAGAACTGTGGGCTATTGATACACAATGAGTGTTCTCCTTGGACTGAATCATCACCGATGAATGGAAGCTGGAAGTGTGGCCAGTGCAGAGAGTGGCGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000122 GO:0000228 GO:0000785 GO:0000790 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0006139 GO:0006325 GO:0006338 GO:0006351 GO:0006355 GO:0006357 GO:0006725 GO:0006807 GO:0006996 GO:0007154 GO:0007165 GO:0007275 GO:0007399 GO:0007417 GO:0007420 GO:0008134 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009526 GO:0009527 GO:0009536 GO:0009707 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009941 GO:0009987 GO:0010019 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0016020 GO:0016043 GO:0016070 GO:0016589 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019867 GO:0023052 GO:0031010 GO:0031090 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031967 GO:0031968 GO:0031969 GO:0031974 GO:0031975 GO:0031981 GO:0032501 GO:0032502 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0035064 GO:0042170 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043565 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044454 GO:0044464 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0046483 GO:0048513 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051173 GO:0051252 GO:0051253 GO:0051254 GO:0051276 GO:0051716 GO:0060255 GO:0060322 GO:0065007 GO:0070013 GO:0070603 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:0098588 GO:0098805 GO:0140030 GO:0140034 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902494 GO:1902679 GO:1902680 GO:1903506 GO:1903507 GO:1903508 GO:1904949 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDT PHD WHIM1

Protein Analysis

1666

Amino Acids

186.67

Weight (kDa)

5.67

Isoelectric Point (pI)

46.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTM_DIR17_Tudor PF21743 43 - 89 7.2e-14 PTM/DIR17-like, Tudor domain
DDT PF02791 202 - 257 1.7e-15 DDT domain
WHIM1 PF15612 303 - 347 2.4e-08 WSTF, HB1, Itc1p, MBD9 motif 1
PHD PF00628 420 - 461 5.6e-08 PHD-finger
Chromo_PTM PF24294 1051 - 1123 4.7e-32 Membrane-bound transcription factor PTM, chromo domain-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 927, 2121
AatII GACGTC 1 cut(s) 3856
Acc16I TGCGCA 1 cut(s) 3323
Acc36I ACCTGC 1 cut(s) 4306
Acc65I GGTACC 3 cut(s) 581, 1814, 4068
AccB1I GGYRCC 4 cut(s) 191, 581, 1814, 4068
AccB7I CCANNNNNTGG 2 cut(s) 394, 1495
AccII CGCG 4 cut(s) 249, 2772, 3805, 3858
AccIII TCCGGA 1 cut(s) 79
AclWI GGATC 8 cut(s) 410, 703, 1769, 1858, 1964, 1994, 2620, 4340
AcoI YGGCCR 5 cut(s) 857, 1588, 2315, 3898, 4975
AcsI RAATTY 5 cut(s) 71, 1689, 1961, 2601, 4552
AcuI CTGAAG 6 cut(s) 561, 2370, 3044, 3810, 4098, 4131
AcyI GRCGYC 2 cut(s) 766, 3853
AdeI CACNNNGTG 2 cut(s) 1326, 4532
AfeI AGCGCT 1 cut(s) 2637
AfiI CCNNNNNNNGG 8 cut(s) 394, 578, 1495, 1748, 3213, 3652, 4703, 4958
AflIII ACRYGT 2 cut(s) 3784, 3803
AhlI ACTAGT 2 cut(s) 1802, 3692
AjuI GAANNNNNNNTTGG 2 cut(s) 1860, 1892
AleI CACNNNNGTG 2 cut(s) 811, 4159
Alw21I GWGCWC 7 cut(s) 715, 1385, 1438, 1467, 1503, 4054, 4092
Alw26I GTCTC 7 cut(s) 98, 452, 2029, 2221, 3137, 3690, 3794
Alw44I GTGCAC 1 cut(s) 1381
AlwI GGATC 8 cut(s) 410, 703, 1769, 1858, 1964, 1994, 2620, 4340
AlwNI CAGNNNCTG 2 cut(s) 3552, 3833
Ama87I CYCGRG 2 cut(s) 1123, 2211
Aor13HI TCCGGA 1 cut(s) 79
Aor51HI AGCGCT 1 cut(s) 2637
ApaLI GTGCAC 1 cut(s) 1381
ApoI RAATTY 5 cut(s) 71, 1689, 1961, 2601, 4552
ArsI GACNNNNNNTTYG 4 cut(s) 56, 88, 2831, 2863
Asp700I GAANNNNTTC 4 cut(s) 146, 671, 3536, 4604
Asp718I GGTACC 3 cut(s) 581, 1814, 4068
AspA2I CCTAGG 2 cut(s) 35, 4436
AspLEI GCGC 5 cut(s) 1920, 2595, 2638, 3058, 3324
AsuC2I CCSGG 1 cut(s) 196
AsuII TTCGAA 2 cut(s) 4608, 4725
AvaI CYCGRG 2 cut(s) 1123, 2211
AvaII GGWCC 9 cut(s) 64, 77, 871, 905, 1117, 1362, 2558, 2774, 3756
AvrII CCTAGG 2 cut(s) 35, 4436
AxyI CCTNAGG 2 cut(s) 2068, 2127
BaeGI GKGCMC 2 cut(s) 1385, 4332
BalI TGGCCA 4 cut(s) 859, 1590, 2317, 4977
BanI GGYRCC 4 cut(s) 191, 581, 1814, 4068
BanII GRGCYC 1 cut(s) 4092
BarI GAAGNNNNNNTAC 2 cut(s) 4851, 4883
BbsI GAAGAC 2 cut(s) 2459, 4663
Bbv12I GWGCWC 7 cut(s) 715, 1385, 1438, 1467, 1503, 4054, 4092
BbvCI CCTCAGC 1 cut(s) 3387
BceAI ACGGC 2 cut(s) 1848, 3913
BcgI CGANNNNNNTGC 2 cut(s) 4715, 4749
BciVI GTATCC 1 cut(s) 3981
BclI TGATCA 2 cut(s) 3624, 4792
BcnI CCSGG 1 cut(s) 196
BcoDI GTCTC 7 cut(s) 98, 452, 2029, 2221, 3137, 3690, 3794
BcuI ACTAGT 2 cut(s) 1802, 3692
BfaI CTAG 7 cut(s) 36, 489, 1803, 2549, 3693, 4218, 4437
BfmI CTRYAG 2 cut(s) 351, 3021
BfoI RGCGCY 2 cut(s) 1921, 2639
BfuAI ACCTGC 1 cut(s) 4306
BfuI GTATCC 1 cut(s) 3981
BglII AGATCT 3 cut(s) 1021, 4009, 4636
BlnI CCTAGG 2 cut(s) 35, 4436
BlpI GCTNAGC 1 cut(s) 4425
Bme18I GGWCC 9 cut(s) 64, 77, 871, 905, 1117, 1362, 2558, 2774, 3756
BmeT110I CYCGRG 2 cut(s) 1123, 2211
BpiI GAAGAC 2 cut(s) 2459, 4663
Bpu10I CCTNAGC 2 cut(s) 3111, 3387
Bpu1102I GCTNAGC 1 cut(s) 4425
Bpu14I TTCGAA 2 cut(s) 4608, 4725
BpuEI CTTGAG 2 cut(s) 1487, 2672
BpuMI CCSGG 1 cut(s) 196
BsaAI YACGTR 1 cut(s) 1943
BsaBI GATNNNNATC 2 cut(s) 1239, 4344
BsaHI GRCGYC 2 cut(s) 766, 3853
BsaI GGTCTC 2 cut(s) 2029, 2221
BsaJI CCNNGG 9 cut(s) 35, 2243, 2348, 2935, 4317, 4430, 4436, 4557, 4934
BsaWI WCCGGW 2 cut(s) 10, 79
BsaXI ACNNNNNCTCC 2 cut(s) 95, 125
Bsc4I CCNNNNNNNGG 8 cut(s) 394, 578, 1495, 1748, 3213, 3652, 4703, 4958
Bse118I RCCGGY 1 cut(s) 1920
Bse21I CCTNAGG 2 cut(s) 2068, 2127
Bse3DI GCAATG 5 cut(s) 1900, 2270, 2505, 2670, 4856
Bse8I GATNNNNATC 2 cut(s) 1239, 4344
BseAI TCCGGA 1 cut(s) 79
BseDI CCNNGG 9 cut(s) 35, 2243, 2348, 2935, 4317, 4430, 4436, 4557, 4934
BseJI GATNNNNATC 2 cut(s) 1239, 4344
BseLI CCNNNNNNNGG 8 cut(s) 394, 578, 1495, 1748, 3213, 3652, 4703, 4958
BseMI GCAATG 5 cut(s) 1900, 2270, 2505, 2670, 4856
BseRI GAGGAG 2 cut(s) 2546, 3404
BseSI GKGCMC 2 cut(s) 1385, 4332
BseYI CCCAGC 1 cut(s) 356
BsgI GTGCAG 1 cut(s) 3565
Bsh1236I CGCG 4 cut(s) 249, 2772, 3805, 3858
BshNI GGYRCC 4 cut(s) 191, 581, 1814, 4068
BsiHKAI GWGCWC 7 cut(s) 715, 1385, 1438, 1467, 1503, 4054, 4092
BsiHKCI CYCGRG 2 cut(s) 1123, 2211
BsiSI CCGG 4 cut(s) 11, 80, 195, 1921
BslFI GGGAC 2 cut(s) 536, 3461
BslI CCNNNNNNNGG 8 cut(s) 394, 578, 1495, 1748, 3213, 3652, 4703, 4958
BsmAI GTCTC 7 cut(s) 98, 452, 2029, 2221, 3137, 3690, 3794
BsmBI CGTCTC 1 cut(s) 3794
BsmFI GGGAC 2 cut(s) 536, 3461
BsmI GAATGC 5 cut(s) 2809, 3324, 3536, 3538, 4423
Bso31I GGTCTC 2 cut(s) 2029, 2221
BsoBI CYCGRG 2 cut(s) 1123, 2211
Bsp119I TTCGAA 2 cut(s) 4608, 4725
Bsp1286I GDGCHC 8 cut(s) 715, 1385, 1438, 1467, 1503, 4054, 4092, 4332
Bsp13I TCCGGA 1 cut(s) 79
Bsp1720I GCTNAGC 1 cut(s) 4425
Bsp19I CCATGG 2 cut(s) 4317, 4557
Bsp68I TCGCGA 1 cut(s) 249
BspEI TCCGGA 1 cut(s) 79
BspFNI CGCG 4 cut(s) 249, 2772, 3805, 3858
BspHI TCATGA 3 cut(s) 2812, 3568, 4492
BspMAI CTGCAG 2 cut(s) 355, 3025
BspMI ACCTGC 1 cut(s) 4306
BspPI GGATC 8 cut(s) 410, 703, 1769, 1858, 1964, 1994, 2620, 4340
BspQI GCTCTTC 1 cut(s) 3508
BspT104I TTCGAA 2 cut(s) 4608, 4725
BspT107I GGYRCC 4 cut(s) 191, 581, 1814, 4068
BspTNI GGTCTC 2 cut(s) 2029, 2221
BsrDI GCAATG 5 cut(s) 1900, 2270, 2505, 2670, 4856
BsrFI RCCGGY 1 cut(s) 1920
BssAI RCCGGY 1 cut(s) 1920
BssECI CCNNGG 9 cut(s) 35, 2243, 2348, 2935, 4317, 4430, 4436, 4557, 4934
BssNI GRCGYC 2 cut(s) 766, 3853
BssT1I CCWWGG 6 cut(s) 35, 2348, 4317, 4436, 4557, 4934
Bst6I CTCTTC 6 cut(s) 1155, 3254, 3508, 3603, 4356, 4467
BstACI GRCGYC 2 cut(s) 766, 3853
BstAPI GCANNNNNTGC 2 cut(s) 2174, 3639
BstBAI YACGTR 1 cut(s) 1943
BstBI TTCGAA 2 cut(s) 4608, 4725
BstDSI CCRYGG 2 cut(s) 4317, 4557
BstEII GGTNACC 1 cut(s) 4706
BstENI CCTNNNNNAGG 1 cut(s) 576
BstFNI CGCG 4 cut(s) 249, 2772, 3805, 3858
BstH2I RGCGCY 2 cut(s) 1921, 2639
BstHHI GCGC 5 cut(s) 1920, 2595, 2638, 3058, 3324
BstMAI GTCTC 7 cut(s) 98, 452, 2029, 2221, 3137, 3690, 3794
BstNSI RCATGY 5 cut(s) 1045, 2064, 2102, 2106, 3788
BstPI GGTNACC 1 cut(s) 4706
BstSFI CTRYAG 2 cut(s) 351, 3021
BstSLI GKGCMC 2 cut(s) 1385, 4332
BstSNI TACGTA 1 cut(s) 1943
BstUI CGCG 4 cut(s) 249, 2772, 3805, 3858
BstV2I GAAGAC 2 cut(s) 2459, 4663
BstX2I RGATCY 5 cut(s) 1021, 1774, 1863, 4009, 4636
BstXI CCANNNNNNTGG 2 cut(s) 4718, 4904
BstYI RGATCY 5 cut(s) 1021, 1774, 1863, 4009, 4636
Bsu36I CCTNAGG 2 cut(s) 2068, 2127
BsuI GTATCC 1 cut(s) 3981
BtgI CCRYGG 2 cut(s) 4317, 4557
BtsI GCAGTG 3 cut(s) 1909, 3434, 3644
BtuMI TCGCGA 1 cut(s) 249
BveI ACCTGC 1 cut(s) 4306
CaiI CAGNNNCTG 2 cut(s) 3552, 3833
CciI TCATGA 3 cut(s) 2812, 3568, 4492
CfoI GCGC 5 cut(s) 1920, 2595, 2638, 3058, 3324
Cfr10I RCCGGY 1 cut(s) 1920
CseI GACGC 3 cut(s) 755, 2244, 3811
CsiI ACCWGGT 1 cut(s) 2956
CspCI CAANNNNNGTGG 2 cut(s) 546, 581
DraIII CACNNNGTG 2 cut(s) 1326, 4532
EaeI YGGCCR 5 cut(s) 857, 1588, 2315, 3898, 4975
Eam1104I CTCTTC 6 cut(s) 1155, 3254, 3508, 3603, 4356, 4467
EarI CTCTTC 6 cut(s) 1155, 3254, 3508, 3603, 4356, 4467
EciI GGCGGA 1 cut(s) 1132
Ecl136II GAGCTC 1 cut(s) 4090
Eco105I TACGTA 1 cut(s) 1943
Eco130I CCWWGG 6 cut(s) 35, 2348, 4317, 4436, 4557, 4934
Eco24I GRGCYC 1 cut(s) 4092
Eco31I GGTCTC 2 cut(s) 2029, 2221
Eco47I GGWCC 9 cut(s) 64, 77, 871, 905, 1117, 1362, 2558, 2774, 3756
Eco47III AGCGCT 1 cut(s) 2637
Eco53kI GAGCTC 1 cut(s) 4090
Eco57I CTGAAG 6 cut(s) 561, 2370, 3044, 3810, 4098, 4131
Eco81I CCTNAGG 2 cut(s) 2068, 2127
Eco88I CYCGRG 2 cut(s) 1123, 2211
Eco91I GGTNACC 1 cut(s) 4706
EcoICRI GAGCTC 1 cut(s) 4090
EcoNI CCTNNNNNAGG 1 cut(s) 576
EcoO109I RGGNCCY 5 cut(s) 64, 905, 1362, 4433, 4787
EcoO65I GGTNACC 1 cut(s) 4706
EcoT14I CCWWGG 6 cut(s) 35, 2348, 4317, 4436, 4557, 4934
EcoT22I ATGCAT 5 cut(s) 2104, 2108, 2154, 3154, 3538
EcoT38I GRGCYC 1 cut(s) 4092
ErhI CCWWGG 6 cut(s) 35, 2348, 4317, 4436, 4557, 4934
Esp3I CGTCTC 1 cut(s) 3794
FalI AAGNNNNNCTT 4 cut(s) 1860, 1892, 2728, 2760
FaqI GGGAC 2 cut(s) 536, 3461
FauI CCCGC 1 cut(s) 3449
FauNDI CATATG 2 cut(s) 2515, 3993
FbaI TGATCA 2 cut(s) 3624, 4792
FriOI GRGCYC 1 cut(s) 4092
FspBI CTAG 7 cut(s) 36, 489, 1803, 2549, 3693, 4218, 4437
FspI TGCGCA 1 cut(s) 3323
GlaI GCGC 5 cut(s) 1919, 2594, 2637, 3057, 3323
GsaI CCCAGC 1 cut(s) 360
HaeII RGCGCY 2 cut(s) 1921, 2639
HapII CCGG 4 cut(s) 11, 80, 195, 1921
HgaI GACGC 3 cut(s) 755, 2244, 3811
HhaI GCGC 5 cut(s) 1920, 2595, 2638, 3058, 3324
Hin1I GRCGYC 2 cut(s) 766, 3853
Hin6I GCGC 5 cut(s) 1918, 2593, 2636, 3056, 3322
HinP1I GCGC 5 cut(s) 1918, 2593, 2636, 3056, 3322
HincII GTYRAC 1 cut(s) 3512
HindII GTYRAC 1 cut(s) 3512
HindIII AAGCTT 2 cut(s) 2333, 2879
HpaII CCGG 4 cut(s) 11, 80, 195, 1921
Hpy166II GTNNAC 8 cut(s) 379, 865, 1120, 1383, 1907, 3512, 4213, 4865
Hpy8I GTNNAC 8 cut(s) 379, 865, 1120, 1383, 1907, 3512, 4213, 4865
Hpy99I CGWCG 2 cut(s) 1004, 3858
HpyCH4IV ACGT 2 cut(s) 1942, 3853
HpySE526I ACGT 2 cut(s) 1942, 3853
Hsp92I GRCGYC 2 cut(s) 766, 3853
HspAI GCGC 5 cut(s) 1918, 2593, 2636, 3056, 3322
Kpn2I TCCGGA 1 cut(s) 79
KpnI GGTACC 3 cut(s) 585, 1818, 4072
KroI GCCGGC 1 cut(s) 1920
KroNI GCCGGC 1 cut(s) 1922
Ksp22I TGATCA 2 cut(s) 3624, 4792
LguI GCTCTTC 1 cut(s) 3508
LmnI GCTCC 5 cut(s) 718, 1433, 1915, 3391, 4057
MabI ACCWGGT 1 cut(s) 2956
MaeI CTAG 7 cut(s) 36, 489, 1803, 2549, 3693, 4218, 4437
MaeII ACGT 2 cut(s) 1942, 3853
MfeI CAATTG 2 cut(s) 563, 1386
MflI RGATCY 5 cut(s) 1021, 1774, 1863, 4009, 4636
MhlI GDGCHC 8 cut(s) 715, 1385, 1438, 1467, 1503, 4054, 4092, 4332
MlsI TGGCCA 4 cut(s) 859, 1590, 2317, 4977
MluI ACGCGT 1 cut(s) 3803
MluNI TGGCCA 4 cut(s) 859, 1590, 2317, 4977
MlyI GAGTC 7 cut(s) 253, 401, 454, 3692, 3839, 4106, 4244
MmeI TCCRAC 8 cut(s) 342, 708, 1042, 2924, 3713, 4121, 4222, 4695
Mox20I TGGCCA 4 cut(s) 859, 1590, 2317, 4977
Mph1103I ATGCAT 5 cut(s) 2104, 2108, 2154, 3154, 3538
MroI TCCGGA 1 cut(s) 79
MroNI GCCGGC 1 cut(s) 1920
MroXI GAANNNNTTC 4 cut(s) 146, 671, 3536, 4604
MscI TGGCCA 4 cut(s) 859, 1590, 2317, 4977
MslI CAYNNNNRTG 7 cut(s) 590, 811, 1472, 3541, 4159, 4190, 4629
Msp20I TGGCCA 4 cut(s) 859, 1590, 2317, 4977
MspA1I CMGCKG 2 cut(s) 356, 3386
MspI CCGG 4 cut(s) 11, 80, 195, 1921
MunI CAATTG 2 cut(s) 563, 1386
Mva1269I GAATGC 5 cut(s) 2809, 3324, 3536, 3538, 4423
MvnI CGCG 4 cut(s) 249, 2772, 3805, 3858
NaeI GCCGGC 1 cut(s) 1922
NciI CCSGG 1 cut(s) 196
NcoI CCATGG 2 cut(s) 4317, 4557
NdeI CATATG 2 cut(s) 2515, 3993
NgoMIV GCCGGC 1 cut(s) 1920
NmuCI GTSAC 5 cut(s) 473, 1734, 1798, 3739, 4706
NruI TCGCGA 1 cut(s) 249
NsbI TGCGCA 1 cut(s) 3323
NsiI ATGCAT 5 cut(s) 2104, 2108, 2154, 3154, 3538
NspI RCATGY 5 cut(s) 1045, 2064, 2102, 2106, 3788
NspV TTCGAA 2 cut(s) 4608, 4725
OliI CACNNNNGTG 2 cut(s) 811, 4159
PaeI GCATGC 2 cut(s) 2102, 2106
PagI TCATGA 3 cut(s) 2812, 3568, 4492
PciI ACATGT 1 cut(s) 3784
PciSI GCTCTTC 1 cut(s) 3508
PcsI WCGNNNNNNNCGW 2 cut(s) 222, 253
PctI GAATGC 5 cut(s) 2809, 3324, 3536, 3538, 4423
PdiI GCCGGC 1 cut(s) 1922
PdmI GAANNNNTTC 4 cut(s) 146, 671, 3536, 4604
PflFI GACNNNGTC 1 cut(s) 1004
PflMI CCANNNNNTGG 2 cut(s) 394, 1495
PfoI TCCNGGA 3 cut(s) 387, 4513, 4776
PleI GAGTC 7 cut(s) 252, 401, 454, 3691, 3838, 4105, 4244
PpsI GAGTC 7 cut(s) 252, 401, 454, 3691, 3838, 4105, 4244
Ppu21I YACGTR 1 cut(s) 1943
PpuMI RGGWCCY 3 cut(s) 64, 905, 1362
PscI ACATGT 1 cut(s) 3784
PsiI TTATAA 2 cut(s) 927, 2121
Psp124BI GAGCTC 1 cut(s) 4092
Psp5II RGGWCCY 3 cut(s) 64, 905, 1362
PspEI GGTNACC 1 cut(s) 4706
PspFI CCCAGC 1 cut(s) 356
PspPPI RGGWCCY 3 cut(s) 64, 905, 1362
PsrI GAACNNNNNNTAC 2 cut(s) 3571, 3603
PstI CTGCAG 2 cut(s) 355, 3025
PstNI CAGNNNCTG 2 cut(s) 3552, 3833
PsuI RGATCY 5 cut(s) 1021, 1774, 1863, 4009, 4636
PsyI GACNNNGTC 1 cut(s) 1004
PvuII CAGCTG 2 cut(s) 356, 3386
RruI TCGCGA 1 cut(s) 249
RseI CAYNNNNRTG 7 cut(s) 590, 811, 1472, 3541, 4159, 4190, 4629
SacI GAGCTC 1 cut(s) 4092
SapI GCTCTTC 1 cut(s) 3508
SchI GAGTC 7 cut(s) 253, 401, 454, 3692, 3839, 4106, 4244
SduI GDGCHC 8 cut(s) 715, 1385, 1438, 1467, 1503, 4054, 4092, 4332
SexAI ACCWGGT 1 cut(s) 2956
SfcI CTRYAG 2 cut(s) 351, 3021
SfuI TTCGAA 2 cut(s) 4608, 4725
SinI GGWCC 9 cut(s) 64, 77, 871, 905, 1117, 1362, 2558, 2774, 3756
SmiMI CAYNNNNRTG 7 cut(s) 590, 811, 1472, 3541, 4159, 4190, 4629
SmlI CTYRAG 2 cut(s) 1502, 2651
SmoI CTYRAG 2 cut(s) 1502, 2651
SnaBI TACGTA 1 cut(s) 1943
SpeI ACTAGT 2 cut(s) 1802, 3692
SphI GCATGC 2 cut(s) 2102, 2106
SspI AATATT 3 cut(s) 1443, 1637, 4311
SspMI CTAG 7 cut(s) 36, 489, 1803, 2549, 3693, 4218, 4437
SstI GAGCTC 1 cut(s) 4092
StyI CCWWGG 6 cut(s) 35, 2348, 4317, 4436, 4557, 4934
TaiI ACGT 2 cut(s) 1945, 3856
TaqI TCGA 5 cut(s) 332, 405, 447, 4608, 4725
TaqII GACCGA 1 cut(s) 1722
TatI WGTACW 2 cut(s) 2057, 3578
TauI GCSGC 2 cut(s) 571, 3861
TseFI GTSAC 5 cut(s) 473, 1734, 1798, 3739, 4706
Tsp45I GTSAC 5 cut(s) 473, 1734, 1798, 3739, 4706
TspGWI ACGGA 3 cut(s) 613, 2612, 3769
Tth111I GACNNNGTC 1 cut(s) 1004
Van91I CCANNNNNTGG 2 cut(s) 394, 1495
VneI GTGCAC 1 cut(s) 1381
VpaK11BI GGWCC 9 cut(s) 64, 77, 871, 905, 1117, 1362, 2558, 2774, 3756
XagI CCTNNNNNAGG 1 cut(s) 576
XapI RAATTY 5 cut(s) 71, 1689, 1961, 2601, 4552
XceI RCATGY 5 cut(s) 1045, 2064, 2102, 2106, 3788
XcmI CCANNNNNNNNNTGG 1 cut(s) 1362
XmaJI CCTAGG 2 cut(s) 35, 4436
XmnI GAANNNNTTC 4 cut(s) 146, 671, 3536, 4604
XspI CTAG 7 cut(s) 36, 489, 1803, 2549, 3693, 4218, 4437
ZraI GACGTC 1 cut(s) 3854
Zsp2I ATGCAT 5 cut(s) 2104, 2108, 2154, 3154, 3538
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.