Rw5G034220

WSTF, HB1, Itc1p, MBD9 motif 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0007748
Physical Location & Seq
Forward (+)
58866501 .. 58873787
7287 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G034220.1

Sequence Viewer

Length: 5055 bp
ATGGATCCTCCGGTGGCGAAAAAGAGGGGCCGGCCGAGGAAGCGGCGGGCTGATGATCGGAAAGCGGGCCCCGAGGCGAAGAAGCAGGCATTGGAGACGAGATCGATTGCGTTGTTGGGGCGGTACGTGTTGAAGGAGTTTGAAAATAGGATTTATCTGGGGAAGGTTGCGTTTTATGATGCTGGATTGTATAGAGTGCACTATGAAGATGGGGATTTCGAGGACTTGGAGAGTGGTGAGATTCGGGGCATTCTTGTGGGTGAGAGTGATTTTGATGGGAGTTTGAGTGCTAAGAGGAAGAAATTGGATAAGCTGGTGGCGAAAGTGAGTGACGAAAAGAAGGTTAAGGTGGATGAGAATGTGGAAAAGGATGTGGTGGTGGTGGATAGGGTTGAATCCTCGGAATTGAGTGAGTGGAGCGGTAGAGTAACTATTGACAATGATGAGATTCGAGAGGATGGTGATGCTGATTTGTCTAGTGATTCGAGTGAGTGTGCAGAGGATATGGATGCCGGGGTTGATGTTGAGACCCCGGCTGTGCCGCTGCCGGAGTTGCCACCGTCTTCTGGAACCATAGGCGTGCCGGAGCAGTGTGTTTCGCATCTTTTCTCGGTGTATGGTTTCATGCGGTCGTTTAGCATCTGCCTGTTCTTGAACCCGTTTACTTTGGATGATTTTGTGGGTTCGCTTAATTGTCGTGCTCCCAACACATTGTTTGATGCAATTCATGTTGCTTTGTTGCGTGCCTTGAGACGTCATCTTGAAACAATCTCATCTGAAGGCTCAGAGGTAGCACAAAAATGTTTGAGGTGCATAGATTGGAGCTTGCTCGATACAGTGACTTGGCCTGTTTACTTGGTCCAGTATTTAACAATTATGGGATATGTAAAAGGACAAGAGTGGAAAGGTTTCTATGATGAGGTTTTGGACAGAGAGTATTATTTATTATCAGTTGATAGGAAGTTAAGCATTTTGCAAATCATTTGTGATGATGTCTTAGACAGTATAGATATTAGAGCTGAATTAGACATGCGTGAAGAATCAGAAGTTGGAATAGATTATGATGCCGAAGCAACCAGTGCTCTTGTAAATGGGCCAAGAAGGGTCCATCCGAGATATTCCAAAACTTCTGCATGCAAGGACAGAGAAGCAATGGAAATTGCTACTGAATCCCATGATACAAAATTATCTGGTAACTTAAATTCACTAGGTTCCAAAGTTGCTAAAGGGGAGTTTGATGCTACTGATGTTGATTCGGATTGTAACTCTGATGAATGTCGGCTTTGTGGTATGGATGGGACATTGCTTTGCTGTGATGGATGCCCATCTGCCTACCATACGAGGTGTATAGGTGTGATGAAACTGTCGATACCAGAAGGATCCTGGTATTGTCCTGAGTGCACTATCAACAAAATTGGGCCAACTATCACAGTTGGAACATCAGTTAAAGGAGCACAATTGTTTGGGATAGATACCTACGAGCACATCTTCATGGGACTTGCAACCACTTACTTGTGTATGCTTAAGGAAACTATTAACACACAACCATGTCTTAGATATTACAATCAGCATGACATTCCAAAAGTCCTGAAAGTTCTTTGTTCATCTGGGCAAAATACATCTTTTTATCTGGGCTTGTGCAATGCAATTTTAGAATATTGGAATATCCCAGAAAGCATCTTATCTCTTTCAGAGCTGGCTGAATCAATGACTAATTTGGCGAAGATAAAGGAAGATGCGAACTTTTGTGGCCAGTCACTTCCTTTGTCTGGCAAGGATTGTGATAAAGTTATGGATATGGTTGAGGCTGGGAACTGTGAAACTTATGTAAATGAAGACCATTTAGGTAAAGCAGTTTCATCTTTCGATTGCCATGGTGATAGTACACCACAGGAGTACTCACATAGGAATATGGAGATTGATGAAAGGAATAAAATGGAATCTGCTGTTTCGACTGGTTCTGTCACCCAGCAAGCTGATCCATCTTACTTGGTCCACAGAAGCTCAGTTGACAGATCAACTGCAATTGACCGAAGCACATGCACACCAGGGAACATCAACAGTGGTAACAAAGTGCATGCAAATGGTATGCGTTTTTCAGTAACTTCACAAAATGAAGAAGGCAATCATATAGGAAAGGTTTACTCCACTTCAGTTGATGATTGCAAATACAAGGGTTCTTTCTTTAAACCCCATGCATACATAAATTACTATGTGCATGGGGATTTTGCTGCATCTGCTGCTGCCAAACTGGCTGTACTTTCATCAGAAGAAGCTCGGGGTTCAGATGTCCAGGCTTCAGGCAATCCCCGGAAAGTTGCATCATCAAACAATTTATGGCAAGCAAAAGCCTTCTCACTAACAGCCTCACGTTTCTTCTGGCCAAGTTTTGAAAAGAAGCTTGTGGAGGTTCCAAGAGAGAGGTGTGGTTGGTGTCTTTCTTGTAAGGCTCCAGTTGCAAGCAAGAGAGGATGCATGCTAAATCATGCTTGTTTGAGTGCCACTAAAGGTGCTATGAAGATCCTTGCCAGCCTTCGTCCTATAAAGAGTGTGGAGGGGAATCTTGTAAGCATCGCAACATACATCTTATACATGGAGGAGAGTTTGCATGGTCTAATAACTGGCCCCTTTCTCAATGAAAATTTTAGGAAACAATTCCGCCAACAAGTTTATGAAGCATCAACCTGCAGTGCAATAAAAGTCCTATTGCTCAAACTTGAGGAAAACATCCGAACAATTGCTCTTTCCGGGGAGTGGATTAAACTGGTAGATGATTTGCTGGTTGAATCTTCTGTGATTCAAGGTCCTACGTGCACTGTTGGAACAACACAAAGACGTGCACCATACAGCAGGCGGAGCAGGAAACAGAGTGCAATTCAAGAAGTTATAGATGATGACTGCAATGACAAGAGTTTTGTATGGTGGCAAGGAGGGAAGCTGTCAAAAATCATATTCCAGAGAGCAATCTTGGCTCGCTCATTGGTTAAAAAAGCTGCTCGCCAAGGTGGATCGAGGAAAATTTTTGGTGTATCTTACGCTGATGGTCCTGATATTCCTAAAAGAAGCAGACAATCTGTATGGAGGGCTGCAGTTGAACTGAGCAAGAACGGATCCCAGCTTGCAGTTCAGGTTAGATACTTGGACTATCATTTGAGGTGGAGTGATCTTGTTCGTCCTGAGCAGAACCTCCTTGATGGGAAAGCTGCAGAGGCAGAAGCTTCTGCTTTTAGAAATGCAAGTATATGTGATAAAAAAATGTTAAAGAACAACATCGTCTATGGGGTTGCATTTGGTAGTCAAAAGCATCTGCCTAACCGTGTAATGAAAAATATCATTGAGACAGAGCAGAATCAGGATGGAACTAACAAGTTCTGGTTTCTTGAATCACGTATTCCTTTATATTTGATTAAGGAATATGAAGAAAGTGTAAGTAAAGTGTCTATTCCATTAGCTCAGGAGCCTAATCTACTGCACAAGTTAAAGAGAAGGCAGCGGAAAGCAACCCGCAGGGACATATTTTTTTATCTCGAGTGTAAAAGAGACAATCTGGACTTGTCTACCTGTTCTATGTGTCAACTGGATATTTTATTGCGCAATGCAGTCAAGTGCAGTGCCTGTCAAGGTTATTGCCATGAGGGCTGTACCATAAGTTCAACAGTTTCTACAAATGAGGAAGTTGAGTTCTTGATCACTTGCAAGCGGTGCTACCATGCGAAAGCTCTTGTCCAGAAGCAAAAGTTTAAGGAGTTTCCAACTAGTCCTTTGCCCTTGCAAATGAAAGAATATCATACTCCACTGACAGTCACAACTGGTGGAAGGCCTAAAAATTATAACCAATCATTAACCTCCATCAGGGTTCAGGAGCCTCGTTCTGAGATTAAACAAGCTACTAGAGACTCGGGTTTGGCAACAAAAAAACGACGTCCCATTTGTTCTTGGGGTGTTATATGGAAGAAGAAGACTCCGGAAACTGGCACCGACTTCAGGATTAATAACATACTCCTCGGGGGTCGTTCGGATGTGCATGGGTTGAAACCTGTTTGCCACTTGTGCCATATGCCATATCTCTCCGATCTGACATATATACGCTGTGAATCTTGCAAAAATTGGTACCATGCTGAAGCTGTCGAACTTGAGGAGTCAAAGATCTGTGAAGTGGCAGGCTTCAAGTGTTGCAAGTGTCGCAGGATAAAATCGCCTCTTTGTCCTTATACTGATCTTAAAGACAAGACCTTGCAAGAGAGTAAGAAGATTCGCAATAAGCGTTCAAAGCAAGAAAATCTTGGAGAGGACTCAGATTCTGCAACCAATTTTGATTCAGAGGTGTTTGAACCTGCTACTCCAGTGTTTCCCATGGAGGAAGTCTCCATTCAGGATGATGATCCTCTTCTTTTTGCTCTCTCAAGAGTGGAGCTAGTTCCAGAACACAATTCAGAAGTTAATGCTGAATGGGATACTGCTGGGCCAGGGCCACGAAAATTACCTGTGAGAAGGCATGTTAAGCGTGAAGAGGACTTGGATATATATTGTGACGGTAATAAATCTCATGCTGAAAAAACTATGCATGATGAAACAAATTACCTGTCGGAACCGATGGAGATTGCGGCATTCCCTCATGTGGAATGGGATACTTCCATGAACAGCATCAATGGTGAGATAATGGAACAGTATGAAGATCTGAACTACGATATGATGGAACCGCAAACTGTCTTTACTATCAATGAGTTGCTGGCACCTGATGACGGCGACCTTTTTGATGGAGTTGAAACATTTGCGGATTTACCAGAAAATCTGGAAAATCCATATACAACTTCACAGCATGTAGGCCCTGAGCAATATGATATGGCCACTTTCCCAGACGAATCAAAGTCTGCAATTCCTGAAACATCTGCTGTTAATATGATGCAATGTCAGATATGTTTACATGCAGAACCAGCTCCTGATCTTTCTTGCCAGAACTGTGGGCTATTAATTCATAACCGTTGTTCCCCTTGGATTGAGTCATCATCTCAGAATAATAGCTGGAAGTGTGGACAGTGCCGGGAGTGGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000122 GO:0000228 GO:0000785 GO:0000790 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0006139 GO:0006325 GO:0006338 GO:0006351 GO:0006355 GO:0006357 GO:0006725 GO:0006807 GO:0006996 GO:0007154 GO:0007165 GO:0007275 GO:0007399 GO:0007417 GO:0007420 GO:0008134 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009526 GO:0009527 GO:0009536 GO:0009707 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009941 GO:0009987 GO:0010019 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0016020 GO:0016043 GO:0016070 GO:0016589 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019867 GO:0023052 GO:0031010 GO:0031090 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031967 GO:0031968 GO:0031969 GO:0031974 GO:0031975 GO:0031981 GO:0032501 GO:0032502 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0035064 GO:0042170 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043565 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044454 GO:0044464 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0046483 GO:0048513 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051173 GO:0051252 GO:0051253 GO:0051254 GO:0051276 GO:0051716 GO:0060255 GO:0060322 GO:0065007 GO:0070013 GO:0070603 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:0098588 GO:0098805 GO:0140030 GO:0140034 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902494 GO:1902679 GO:1902680 GO:1903506 GO:1903507 GO:1903508 GO:1904949 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDT PHD WHIM1

Protein Analysis

1684

Amino Acids

189.2

Weight (kDa)

5.71

Isoelectric Point (pI)

46.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTM_DIR17_Tudor PF21743 40 - 85 4.6e-15 PTM/DIR17-like, Tudor domain
DDT PF02791 197 - 253 5e-18 DDT domain
WHIM1 PF15612 299 - 343 8.7e-10 WSTF, HB1, Itc1p, MBD9 motif 1
PHD PF00628 426 - 467 5.7e-08 PHD-finger
Chromo_PTM PF24294 1073 - 1145 4.5e-33 Membrane-bound transcription factor PTM, chromo domain-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3831
AatII GACGTC 2 cut(s) 757, 3925
Acc16I TGCGCA 1 cut(s) 3593
Acc36I ACCTGC 2 cut(s) 2693, 4342
Acc65I GGTACC 1 cut(s) 4110
AccB1I GGYRCC 3 cut(s) 3974, 4110, 4732
AccBSI CCGCTC 1 cut(s) 420
AccI GTMKAC 1 cut(s) 3557
AccIII TCCGGA 1 cut(s) 3964
AclWI GGATC 9 cut(s) 12, 1374, 1387, 1973, 2515, 3016, 3105, 3118, 4376
AcoI YGGCCR 4 cut(s) 32, 1750, 2381, 4845
AcsI RAATTY 3 cut(s) 1201, 2641, 3018
AcuI CTGAAG 5 cut(s) 798, 2136, 2283, 3967, 4140
AcyI GRCGYC 2 cut(s) 754, 3922
AfaI GTAC 6 cut(s) 125, 1885, 1898, 2259, 3643, 4112
AfiI CCNNNNNNNGG 8 cut(s) 566, 1769, 2754, 3852, 3971, 3984, 4618, 4742
AflII CTTAAG 1 cut(s) 1523
AflIII ACRYGT 1 cut(s) 126
AhlI ACTAGT 1 cut(s) 3755
AjiI CACGTC 1 cut(s) 2837
AjnI CCWGG 4 cut(s) 1382, 2047, 2292, 4465
AjuI GAANNNNNNNTTGG 4 cut(s) 74, 106, 1032, 1064
AloI GAACNNNNNNTCC 2 cut(s) 3665, 3697
Alw21I GWGCWC 8 cut(s) 201, 703, 1084, 1403, 1456, 1485, 2816, 2842
Alw26I GTCTC 7 cut(s) 89, 521, 745, 3332, 3534, 3888, 4369
Alw44I GTGCAC 4 cut(s) 197, 1399, 2812, 2838
AlwI GGATC 9 cut(s) 12, 1374, 1387, 1973, 2515, 3016, 3105, 3118, 4376
AlwNI CAGNNNCTG 3 cut(s) 3615, 4301, 4940
Ama87I CYCGRG 5 cut(s) 71, 2277, 3527, 3898, 4004
Aor13HI TCCGGA 1 cut(s) 3964
ApaI GGGCCC 1 cut(s) 71
ApaLI GTGCAC 4 cut(s) 197, 1399, 2812, 2838
ApeKI GCWGC 8 cut(s) 544, 2231, 2240, 2243, 2993, 3086, 3203, 3490
ApoI RAATTY 3 cut(s) 1201, 2641, 3018
ArsI GACNNNNNNTTYG 6 cut(s) 2897, 2929, 3912, 3944, 4568, 4600
AseI ATTAAT 2 cut(s) 3990, 4970
Asp700I GAANNNNTTC 2 cut(s) 908, 2654
Asp718I GGTACC 1 cut(s) 4110
AspLEI GCGC 1 cut(s) 3594
AsuC2I CCSGG 5 cut(s) 514, 533, 2311, 2749, 5042
AsuHPI GGTGA 6 cut(s) 248, 272, 473, 1889, 1957, 4664
AvaI CYCGRG 5 cut(s) 71, 2277, 3527, 3898, 4004
AvaII GGWCC 5 cut(s) 859, 1105, 1993, 2804, 3044
BaeGI GKGCMC 5 cut(s) 71, 201, 1403, 2816, 2842
BalI TGGCCA 3 cut(s) 1752, 2383, 4847
BamHI GGATCC 3 cut(s) 4, 1379, 3110
BanI GGYRCC 3 cut(s) 3974, 4110, 4732
BanII GRGCYC 1 cut(s) 71
BbsI GAAGAC 3 cut(s) 555, 1842, 3965
Bbv12I GWGCWC 8 cut(s) 201, 703, 1084, 1403, 1456, 1485, 2816, 2842
BbvI GCAGC 8 cut(s) 531, 2218, 2227, 2230, 2980, 3073, 3190, 3502
BceAI ACGGC 1 cut(s) 4759
BcgI CGANNNNNNTGC 2 cut(s) 2022, 2056
BciT130I CCWGG 4 cut(s) 1384, 2049, 2294, 4467
BciVI GTATCC 2 cut(s) 4447, 4621
BclI TGATCA 1 cut(s) 3687
BcnI CCSGG 5 cut(s) 514, 533, 2311, 2749, 5042
BcoDI GTCTC 7 cut(s) 89, 521, 745, 3332, 3534, 3888, 4369
BcuI ACTAGT 1 cut(s) 3755
BfaI CTAG 5 cut(s) 477, 1208, 3756, 3891, 4415
BfmI CTRYAG 3 cut(s) 2686, 3087, 3204
BfrI CTTAAG 1 cut(s) 1523
BfuAI ACCTGC 2 cut(s) 2693, 4342
BfuI GTATCC 2 cut(s) 4447, 4621
BglI GCCNNNNNGGC 2 cut(s) 2252, 3636
BglII AGATCT 2 cut(s) 4146, 4675
BmcAI AGTACT 1 cut(s) 1898
Bme1390I CCNGG 9 cut(s) 514, 533, 1384, 2049, 2294, 2311, 2749, 4467, 5042
Bme18I GGWCC 5 cut(s) 859, 1105, 1993, 2804, 3044
BmeT110I CYCGRG 5 cut(s) 71, 2277, 3527, 3898, 4004
BmgBI CACGTC 1 cut(s) 2837
BmrFI CCNGG 9 cut(s) 514, 533, 1384, 2049, 2294, 2311, 2749, 4467, 5042
BpiI GAAGAC 3 cut(s) 555, 1842, 3965
BplI GAGNNNNNCTC 2 cut(s) 4349, 4381
BpmI CTGGAG 2 cut(s) 2436, 4326
Bpu10I CCTNAGC 3 cut(s) 3177, 3453, 4830
BpuEI CTTGAG 4 cut(s) 769, 2738, 4154, 4387
BpuMI CCSGG 5 cut(s) 514, 533, 2311, 2749, 5042
Bsa29I ATCGAT 1 cut(s) 104
BsaAI YACGTR 3 cut(s) 127, 2811, 3389
BsaBI GATNNNNATC 2 cut(s) 1324, 4380
BsaHI GRCGYC 2 cut(s) 754, 3922
BsaI GGTCTC 1 cut(s) 521
BsaWI WCCGGW 2 cut(s) 10, 3964
BsaXI ACNNNNNCTCC 3 cut(s) 271, 301, 5036
Bsc4I CCNNNNNNNGG 8 cut(s) 566, 1769, 2754, 3852, 3971, 3984, 4618, 4742
Bse118I RCCGGY 1 cut(s) 30
Bse3DI GCAATG 6 cut(s) 1158, 1301, 1648, 2908, 3601, 4913
Bse8I GATNNNNATC 2 cut(s) 1324, 4380
BseAI TCCGGA 1 cut(s) 3964
BseBI CCWGG 4 cut(s) 1384, 2049, 2294, 4467
BseCI ATCGAT 1 cut(s) 104
BseJI GATNNNNATC 2 cut(s) 1324, 4380
BseLI CCNNNNNNNGG 8 cut(s) 566, 1769, 2754, 3852, 3971, 3984, 4618, 4742
BseMI GCAATG 6 cut(s) 1158, 1301, 1648, 2908, 3601, 4913
BseRI GAGGAG 3 cut(s) 2612, 3992, 4151
BseSI GKGCMC 5 cut(s) 71, 201, 1403, 2816, 2842
BseX3I CGGCCG 1 cut(s) 32
BseXI GCAGC 8 cut(s) 531, 2218, 2227, 2230, 2980, 3073, 3190, 3502
BseYI CCCAGC 4 cut(s) 1808, 1968, 3114, 4460
BsgI GTGCAG 3 cut(s) 516, 3455, 3628
Bsh1285I CGRYCG 2 cut(s) 35, 632
BshNI GGYRCC 3 cut(s) 3974, 4110, 4732
BshVI ATCGAT 1 cut(s) 104
BsiEI CGRYCG 2 cut(s) 35, 632
BsiHKAI GWGCWC 8 cut(s) 201, 703, 1084, 1403, 1456, 1485, 2816, 2842
BsiHKCI CYCGRG 5 cut(s) 71, 2277, 3527, 3898, 4004
BslFI GGGAC 4 cut(s) 1312, 1509, 3524, 3909
BslI CCNNNNNNNGG 8 cut(s) 566, 1769, 2754, 3852, 3971, 3984, 4618, 4742
BsmAI GTCTC 7 cut(s) 89, 521, 745, 3332, 3534, 3888, 4369
BsmBI CGTCTC 2 cut(s) 89, 745
BsmFI GGGAC 4 cut(s) 1312, 1509, 3524, 3909
BsmI GAATGC 2 cut(s) 249, 4607
Bso31I GGTCTC 1 cut(s) 521
BsoBI CYCGRG 5 cut(s) 71, 2277, 3527, 3898, 4004
Bsp120I GGGCCC 1 cut(s) 67
Bsp1286I GDGCHC 9 cut(s) 71, 201, 703, 1084, 1403, 1456, 1485, 2816, 2842
Bsp13I TCCGGA 1 cut(s) 3964
Bsp19I CCATGG 2 cut(s) 1873, 4353
BspDI ATCGAT 1 cut(s) 104
BspEI TCCGGA 1 cut(s) 3964
BspMAI CTGCAG 3 cut(s) 2690, 3091, 3208
BspMI ACCTGC 2 cut(s) 2693, 4342
BspPI GGATC 9 cut(s) 12, 1374, 1387, 1973, 2515, 3016, 3105, 3118, 4376
BspT107I GGYRCC 3 cut(s) 3974, 4110, 4732
BspTI CTTAAG 1 cut(s) 1523
BspTNI GGTCTC 1 cut(s) 521
BsrBI CCGCTC 1 cut(s) 420
BsrDI GCAATG 6 cut(s) 1158, 1301, 1648, 2908, 3601, 4913
BsrFI RCCGGY 1 cut(s) 30
BssAI RCCGGY 1 cut(s) 30
BssNI GRCGYC 2 cut(s) 754, 3922
BssT1I CCWWGG 4 cut(s) 1873, 3001, 4353, 4991
Bst2UI CCWGG 4 cut(s) 1384, 2049, 2294, 4467
Bst6I CTCTTC 2 cut(s) 4392, 4503
BstACI GRCGYC 2 cut(s) 754, 3922
BstAFI CTTAAG 1 cut(s) 1523
BstAPI GCANNNNNTGC 3 cut(s) 1079, 2240, 3702
BstBAI YACGTR 3 cut(s) 127, 2811, 3389
BstDSI CCRYGG 2 cut(s) 1873, 4353
BstENI CCTNNNNNAGG 1 cut(s) 3850
BstHHI GCGC 1 cut(s) 3594
BstMAI GTCTC 7 cut(s) 89, 521, 745, 3332, 3534, 3888, 4369
BstMCI CGRYCG 2 cut(s) 35, 632
BstNI CCWGG 4 cut(s) 1384, 2049, 2294, 4467
BstNSI RCATGY 8 cut(s) 1033, 1137, 2043, 2081, 2479, 4499, 4823, 4928
BstSCI CCNGG 9 cut(s) 512, 531, 1382, 2047, 2292, 2309, 2747, 4465, 5040
BstSFI CTRYAG 3 cut(s) 2686, 3087, 3204
BstSLI GKGCMC 5 cut(s) 71, 201, 1403, 2816, 2842
BstV1I GCAGC 8 cut(s) 531, 2218, 2227, 2230, 2980, 3073, 3190, 3502
BstV2I GAAGAC 3 cut(s) 555, 1842, 3965
BstX2I RGATCY 6 cut(s) 4, 1379, 2520, 3110, 4146, 4675
BstXI CCANNNNNNTGG 1 cut(s) 4961
BstYI RGATCY 6 cut(s) 4, 1379, 2520, 3110, 4146, 4675
BstZI CGGCCG 1 cut(s) 32
Bsu15I ATCGAT 1 cut(s) 104
BsuI GTATCC 2 cut(s) 4447, 4621
BsuTUI ATCGAT 1 cut(s) 104
BtgI CCRYGG 2 cut(s) 1873, 4353
BtgZI GCGATG 1 cut(s) 2557
BtrI CACGTC 1 cut(s) 2837
BtsI GCAGTG 3 cut(s) 596, 2695, 3616
BveI ACCTGC 2 cut(s) 2693, 4342
CaiI CAGNNNCTG 3 cut(s) 3615, 4301, 4940
CfoI GCGC 1 cut(s) 3594
Cfr10I RCCGGY 1 cut(s) 30
ClaI ATCGAT 1 cut(s) 104
Csp6I GTAC 6 cut(s) 124, 1884, 1897, 2258, 3642, 4111
CspCI CAANNNNNGTGG 2 cut(s) 2137, 2172
CviQI GTAC 6 cut(s) 124, 1884, 1897, 2258, 3642, 4111
DraI TTTAAA 1 cut(s) 2188
EaeI YGGCCR 4 cut(s) 32, 1750, 2381, 4845
EagI CGGCCG 1 cut(s) 32
Eam1104I CTCTTC 2 cut(s) 4392, 4503
EarI CTCTTC 2 cut(s) 4392, 4503
EciI GGCGGA 2 cut(s) 2648, 2869
EclXI CGGCCG 1 cut(s) 32
Eco130I CCWWGG 4 cut(s) 1873, 3001, 4353, 4991
Eco147I AGGCCT 1 cut(s) 3820
Eco24I GRGCYC 1 cut(s) 71
Eco31I GGTCTC 1 cut(s) 521
Eco47I GGWCC 5 cut(s) 859, 1105, 1993, 2804, 3044
Eco52I CGGCCG 1 cut(s) 32
Eco57I CTGAAG 5 cut(s) 798, 2136, 2283, 3967, 4140
Eco88I CYCGRG 5 cut(s) 71, 2277, 3527, 3898, 4004
EcoNI CCTNNNNNAGG 1 cut(s) 3850
EcoO109I RGGNCCY 3 cut(s) 68, 2804, 4826
EcoRII CCWGG 4 cut(s) 1382, 2047, 2292, 4465
EcoT14I CCWWGG 4 cut(s) 1873, 3001, 4353, 4991
EcoT22I ATGCAT 3 cut(s) 2200, 2477, 4566
EcoT38I GRGCYC 1 cut(s) 71
ErhI CCWWGG 4 cut(s) 1873, 3001, 4353, 4991
Esp3I CGTCTC 2 cut(s) 89, 745
FalI AAGNNNNNCTT 2 cut(s) 4266, 4298
FaqI GGGAC 4 cut(s) 1312, 1509, 3524, 3909
FauI CCCGC 3 cut(s) 39, 58, 3512
FauNDI CATATG 1 cut(s) 4056
FbaI TGATCA 1 cut(s) 3687
FblI GTMKAC 1 cut(s) 3557
FriOI GRGCYC 1 cut(s) 71
FseI GGCCGGCC 1 cut(s) 34
FspBI CTAG 5 cut(s) 477, 1208, 3756, 3891, 4415
FspI TGCGCA 1 cut(s) 3593
GlaI GCGC 1 cut(s) 3593
GsaI CCCAGC 4 cut(s) 1812, 1972, 3118, 4464
GsuI CTGGAG 2 cut(s) 2436, 4326
HhaI GCGC 1 cut(s) 3594
Hin1I GRCGYC 2 cut(s) 754, 3922
Hin6I GCGC 1 cut(s) 3592
HinP1I GCGC 1 cut(s) 3592
HincII GTYRAC 2 cut(s) 2011, 3575
HindII GTYRAC 2 cut(s) 2011, 3575
HindIII AAGCTT 2 cut(s) 2399, 3216
HphI GGTGA 6 cut(s) 248, 272, 473, 1889, 1957, 4664
Hpy99I CGWCG 1 cut(s) 3924
HpyCH4IV ACGT 7 cut(s) 126, 754, 2371, 2810, 2836, 3388, 3922
HpySE526I ACGT 7 cut(s) 126, 754, 2371, 2810, 2836, 3388, 3922
Hsp92I GRCGYC 2 cut(s) 754, 3922
HspAI GCGC 1 cut(s) 3592
Kpn2I TCCGGA 1 cut(s) 3964
KpnI GGTACC 1 cut(s) 4114
KroI GCCGGC 1 cut(s) 30
KroNI GCCGGC 1 cut(s) 32
Ksp22I TGATCA 1 cut(s) 3687
Lsp1109I GCAGC 8 cut(s) 531, 2218, 2227, 2230, 2980, 3073, 3190, 3502
MaeI CTAG 5 cut(s) 477, 1208, 3756, 3891, 4415
MaeII ACGT 7 cut(s) 126, 754, 2371, 2810, 2836, 3388, 3922
MbiI CCGCTC 1 cut(s) 420
MfeI CAATTG 3 cut(s) 1457, 2025, 2736
MflI RGATCY 6 cut(s) 4, 1379, 2520, 3110, 4146, 4675
MhlI GDGCHC 9 cut(s) 71, 201, 703, 1084, 1403, 1456, 1485, 2816, 2842
MlsI TGGCCA 3 cut(s) 1752, 2383, 4847
MluNI TGGCCA 3 cut(s) 1752, 2383, 4847
MlyI GAGTC 5 cut(s) 3890, 3955, 4148, 4286, 5009
MmeI TCCRAC 5 cut(s) 1030, 1414, 2800, 3776, 4566
Mox20I TGGCCA 3 cut(s) 1752, 2383, 4847
Mph1103I ATGCAT 3 cut(s) 2200, 2477, 4566
MroI TCCGGA 1 cut(s) 3964
MroNI GCCGGC 1 cut(s) 30
MroXI GAANNNNTTC 2 cut(s) 908, 2654
MscI TGGCCA 3 cut(s) 1752, 2383, 4847
MslI CAYNNNNRTG 6 cut(s) 254, 578, 799, 1490, 1546, 2082
Msp20I TGGCCA 3 cut(s) 1752, 2383, 4847
MspA1I CMGCKG 2 cut(s) 544, 3493
MspCI CTTAAG 1 cut(s) 1523
MspR9I CCNGG 9 cut(s) 514, 533, 1384, 2049, 2294, 2311, 2749, 4467, 5042
MunI CAATTG 3 cut(s) 1457, 2025, 2736
Mva1269I GAATGC 2 cut(s) 249, 4607
MvaI CCWGG 4 cut(s) 1384, 2049, 2294, 4467
NaeI GCCGGC 1 cut(s) 32
NciI CCSGG 5 cut(s) 514, 533, 2311, 2749, 5042
NcoI CCATGG 2 cut(s) 1873, 4353
NdeI CATATG 1 cut(s) 4056
NgoMIV GCCGGC 1 cut(s) 30
NmeAIII GCCGAG 1 cut(s) 60
NmuCI GTSAC 6 cut(s) 329, 838, 1755, 1963, 3802, 4529
NsbI TGCGCA 1 cut(s) 3593
NsiI ATGCAT 3 cut(s) 2200, 2477, 4566
NspI RCATGY 8 cut(s) 1033, 1137, 2043, 2081, 2479, 4499, 4823, 4928
PaeI GCATGC 3 cut(s) 1137, 2081, 2479
PaeR7I CTCGAG 1 cut(s) 3527
PceI AGGCCT 1 cut(s) 3820
PcsI WCGNNNNNNNCGW 1 cut(s) 4261
PctI GAATGC 2 cut(s) 249, 4607
PdiI GCCGGC 1 cut(s) 32
PdmI GAANNNNTTC 2 cut(s) 908, 2654
PleI GAGTC 5 cut(s) 3890, 3955, 4147, 4286, 5008
PpsI GAGTC 5 cut(s) 3890, 3955, 4147, 4286, 5008
Ppu21I YACGTR 3 cut(s) 127, 2811, 3389
PpuMI RGGWCCY 1 cut(s) 2804
PshBI ATTAAT 2 cut(s) 3990, 4970
PsiI TTATAA 1 cut(s) 3831
Psp5II RGGWCCY 1 cut(s) 2804
Psp6I CCWGG 4 cut(s) 1382, 2047, 2292, 4465
PspFI CCCAGC 4 cut(s) 1808, 1968, 3114, 4460
PspGI CCWGG 4 cut(s) 1382, 2047, 2292, 4465
PspOMI GGGCCC 1 cut(s) 67
PspPPI RGGWCCY 1 cut(s) 2804
PsrI GAACNNNNNNTAC 2 cut(s) 3634, 3666
PstI CTGCAG 3 cut(s) 2690, 3091, 3208
PstNI CAGNNNCTG 3 cut(s) 3615, 4301, 4940
PsuI RGATCY 6 cut(s) 4, 1379, 2520, 3110, 4146, 4675
RigI GGCCGGCC 1 cut(s) 34
RsaI GTAC 6 cut(s) 125, 1885, 1898, 2259, 3643, 4112
RsaNI GTAC 6 cut(s) 124, 1884, 1897, 2258, 3642, 4111
RseI CAYNNNNRTG 6 cut(s) 254, 578, 799, 1490, 1546, 2082
ScaI AGTACT 1 cut(s) 1898
SchI GAGTC 5 cut(s) 3890, 3955, 4148, 4286, 5009
ScrFI CCNGG 9 cut(s) 514, 533, 1384, 2049, 2294, 2311, 2749, 4467, 5042
SduI GDGCHC 9 cut(s) 71, 201, 703, 1084, 1403, 1456, 1485, 2816, 2842
SfcI CTRYAG 3 cut(s) 2686, 3087, 3204
Sfr274I CTCGAG 1 cut(s) 3527
SinI GGWCC 5 cut(s) 859, 1105, 1993, 2804, 3044
SlaI CTCGAG 1 cut(s) 3527
SmiMI CAYNNNNRTG 6 cut(s) 254, 578, 799, 1490, 1546, 2082
SmlI CTYRAG 6 cut(s) 748, 1523, 2717, 3527, 4133, 4402
SmoI CTYRAG 6 cut(s) 748, 1523, 2717, 3527, 4133, 4402
SpeI ACTAGT 1 cut(s) 3755
SphI GCATGC 3 cut(s) 1137, 2081, 2479
SseBI AGGCCT 1 cut(s) 3820
SspI AATATT 1 cut(s) 1658
SspMI CTAG 5 cut(s) 477, 1208, 3756, 3891, 4415
StuI AGGCCT 1 cut(s) 3820
StyD4I CCNGG 9 cut(s) 512, 531, 1382, 2047, 2292, 2309, 2747, 4465, 5040
StyI CCWWGG 4 cut(s) 1873, 3001, 4353, 4991
TaiI ACGT 7 cut(s) 129, 757, 2374, 2813, 2839, 3391, 3925
TaqII GACCGA 1 cut(s) 2046
TatI WGTACW 3 cut(s) 1883, 1896, 2257
TauI GCSGC 3 cut(s) 46, 544, 4607
TseFI GTSAC 6 cut(s) 329, 838, 1755, 1963, 3802, 4529
TseI GCWGC 8 cut(s) 544, 2231, 2240, 2243, 2993, 3086, 3203, 3490
Tsp45I GTSAC 6 cut(s) 329, 838, 1755, 1963, 3802, 4529
TspGWI ACGGA 1 cut(s) 3123
Vha464I CTTAAG 1 cut(s) 1523
VneI GTGCAC 4 cut(s) 197, 1399, 2812, 2838
VpaK11BI GGWCC 5 cut(s) 859, 1105, 1993, 2804, 3044
VspI ATTAAT 2 cut(s) 3990, 4970
XagI CCTNNNNNAGG 1 cut(s) 3850
XapI RAATTY 3 cut(s) 1201, 2641, 3018
XceI RCATGY 8 cut(s) 1033, 1137, 2043, 2081, 2479, 4499, 4823, 4928
XcmI CCANNNNNNNNNTGG 1 cut(s) 1380
XhoI CTCGAG 1 cut(s) 3527
XmiI GTMKAC 1 cut(s) 3557
XmnI GAANNNNTTC 2 cut(s) 908, 2654
XspI CTAG 5 cut(s) 477, 1208, 3756, 3891, 4415
ZraI GACGTC 2 cut(s) 755, 3923
ZrmI AGTACT 1 cut(s) 1898
Zsp2I ATGCAT 3 cut(s) 2200, 2477, 4566
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.