Rh5AG363900

WSTF, HB1, Itc1p, MBD9 motif 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0007748
Physical Location & Seq
Forward (+)
61502903 .. 61511118
8216 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG363900.1

Sequence Viewer

Length: 4236 bp
ATGGATCCTCCGGTGGCGAAGAAGAGGGGCCGGCCGAGGAAGCGGCGGGCTGATGATCGGAAAGCGGGCCCCGAGGCGAAGAAACAGGCATTGGAGACGAGATCGATTGCGTTGTTGGGGCGGTACGTGTTGAAGGAGTTTGAAAGTAGGATTTATCTGGGGAAAGTTGCGTTTTATGATGCTGGATTGTATAGAGTGCACTATGAAGATGGGGATTTCGAGGACTTGGAGAGTGGTGAGATTCGGGGCATTCTTGTGGGTGAGAGTGATTTTGATGGGAGTTTGAGTGCTAAGAGGAAGAAATTGGATAAGCTGGTGGCGAAAGTGAGTGACGAAAAGAAGGTTAAGGTGGATGAGAATGTGGAAAAGGAGGTGGTGGTGGATAGGGTTGAATCCTCGGAATTGAGTGAGTGGAGCGGTAGAGTAACTATTGACAATGATGAGATTCGAGAGGATGGTGATGCTGATTTGTCTAGTGATTCGAGTGAGTGTGCAGAGGATATGGATGCCGGGGTTGATGTTGAGACCCCGGCCGTGCCGCCCCCGGAGTTGCCACCGTCTTCTGGAACCATAGGCGTGCCGGAGCAGTGTGTTTCGCATCTTTTCTCTGTGTATGGTTTCATGCGGTCGTTTAGCATCTGCCTGTTCTTGAACCCGTTTACTTTGGATGATTTTGTGGGTTCGCTTAATTGTCGTGCTCCAAACACATTGTTTGATGCAATTCATGTTGCTTTGTTGCGTGCCTTGAGACGTCATCTTGAAACAATCTCATCCGAAGGCTCAGAGGTAGCACAAAAATGTTTGAGGTGCATAGATTGGAGCTTGCTCGATACAGTGACTTGGCCTGTTTACTTGGTCCAGTATTTAACAATTATGGGATATGTAAAAGGACAAGAGTGGAAAGGTTTCTATGATGAGGTTTTGGACAGAGAGTATTATTTATTATCAGTTGATAGGAAGTTAAGCATTTTGCAAATCATTTGTGATGATGTCTTAGACAGTATAGATATAAGAGCTGAATTAGACATGCGTGAAGAATCAGAAGTTGGAATAGATTATGATGCCGAAGCAACCAGTGCTCTTGTAAATGGGCCAAGAAGGGTCCATCCGAGATATTCCAAAACTTCTGCATGCAAGGACAGAGAAGCAATGGAAATTGCTACTGAATCCCATGATATAAAATTATCTGGTAACTTAAATTCACTAGGTTCCAAAGTTGCTAAAGGGGAGTTTGATGCTACTGATGTTGATTCGGATTGTAACTCTGATGAATGTCGGCTTTGTGGTATGGATGGGACATTGCTTTGCTGTGATGGATGCCCATCTGCCTACCATACGAGGTGTATAGGTGTGATGAAACTGTCGATACCAGAAGGATCCTGGTATTGTCCTGAGTGCACTATCAACAAAATTGGGCCAACTATCACAGTTGGAACATCAGTTAAAGGAGCACAATTGTTTGGGATAGATGCCTACGAGCACATCTTCATGGGTACTTGCAACCACTTACTTGTGCTTAAGGAAACTATTAACACACAACCATGTCTTAGATATTACAATCAGCATGACATTCCAAAAGTCCTGAAAGTTCTTTGTTCATCTGGGCAAAATACATCTTTTTATCTGGGCTTGTGCAAGGCAATTTTAGAATATTGGAATATCCCAGAAAGCATCTTATCTCTTTCAGAGCTGGCTGAATCAATGACTAATTTGGCGAAGATAAAGGAAGATGCGAACTTTTGTGGCCAGTCACTTCCTTTGTCTGGCAAGGATTGTGATAAAGTTATGGATATGGTTGAGGCTGGGAACTGTGAAACTTATGTAAATGAAGACCATTTAGGTAAAGCAGTTTCATCTTTCGATTGCCATGGTGATAGTACACCACAGGAGTACTCACATAGGAATATGGAGATTGATGAAAGGAATAAAATGGAATCTGCTGTTTCGACTGGTTCTGTCACCCAGCAAGCTGATCCATCTTACTTGGTCCACAGAAGCTCAGTTGACAGATCAACTGCAATTGACCGAAGCACATGCACACCAGGGAACGTCAACAGTGGTAACAAAGTGCATGCAAATGGTATGCGTTTTTCAGTAACTTCACAAAATGAAGAAGGCAATCATATAGGAAAGGTTTACTCCACTTCAGTTGATGATTGCAAATACAAGGGTTCTTTCTTTAAACCCCATGCATACATAAATTACTATGTGCATGGGGATTTTGCTGCATCTGCTGCTGCCAAACTAGCTGTACTTTCATCAGAAGAAGCTCGGGGTTCAGATGTCCAGGCTTCAGGCAATCCCCGGAAAGTTGCATCATCAAACAATTTATGGCAAGCAAAAGCCTTCTCACTAACAGCCTCACGTTTCTTCTGGCCAAGTTTTGAAAAGAAGCTTGTGGAGGTTCCAAGAGAGAGGTGTGGTTGGTGTCTTTCTTGTAAGGCTCCAGTTGCAAGCAAGAGAGGATGCATGCTAAATCATGCTTGTTTGAGTGCCACTAAAGGTGCTATGAAGATCCTTGCCAGCCTTCGTCCTATAAAGAGTGTGGAGGGGAATCTTGTAAGCATCGCAACATACATCTTATACATGGAGGAGAGTCTGCATGGTCTAATAACTGGCCCCTTTCTCAATGAAAATTTTAGGAAACAATTCCGCCAACAAGTTTATGAAGCATCAACCTGCAGTGCAATAAAAGTCCTATTGCTCAAACTTGAGGAAAACATCCGAACAATTGCTCTTTCCGGGGAGTGGATTAAACTGGTAGATGATTTGCTGGTTGAATCTTCTGTGATTCAAGGTCCTACGTGCACTGTTGGAACAACACAAAGACGTGCACCATACAGCAGGCGGAGCAGGAAACAGAGTGCAATTCAAGAAGTTATAGATGATGACTGCAATGACAAGAGTTTTGTATGGTGGCAAGGAGGGAAGCTGTCAAAAATCATATTCCAGAGAGCAATCTTGGCTCGCTCATTGGTTAAAAAAGCAGCTCGCCAAGGTGGATCGAGGAAAATTTTTGGTGTATCTTACGCTGATGGTCCTGATATTCCTAAAAGAAGCAGACAATCTGTATGGAGGGCTGCAGTTGAACTGAGCAAGAACGGATCCCAGCTTGCAGTTCAGGTTAGATACTTGGACTATCATTTGAGGTGGAGTGATCTTGTTCGTCCTGAGCAGAACCTCCTTGATGGGAAAGCTGCAGAGGCAGAAGCTTCTGCTTTTAGAAATGCAAGTATATGTGATCAAAAAATGTTAAAGAACAACATCGTCTATGGGGTTGCATTTGGTAGTCAAAAGCATCTGCCTAACCGTGTAATGAAAAATATCATTGAGACAGAGCAGAATCAGGATGGAACTAACAAGTTCTGGTTTCTTGAATCACGTATTCCTTTATATTTGATTAAGGAATATGAAGAAAGTGTAAGTAAAGTGTCTATTCCATTAGCTCAGGAGCCTAATCTATTGCACAAGTTAAAGAGAAGGCAGCGGAAAGCAACCCGCAGGGACATATTTTTTTATCTCGAGTGTAAAAGAGACAATCTGGACTTGTCTACCTGTTCTATGTGTCAACTGGATATTTTATTGCGCAATGCAGTCAAGTGCAGTGCCTGTCAAGGTTATTGCCATGAGGGCTGTACCATAAGTTCAACAGTTTCTACAAATGAGGAAGTTGAGTTCTTGATCACTTGCAAGCGGTGCTACCATGCGAAAGCTCTTGCCCAGAAGCAAAAGTTTAAGGAGTTTCCAACTAGTCCTTTGCCCTTGCAAATGAAAGAATATCATACTCCACTGACAGTCACAACTGGTGGAAGGCCTAAAAATTATAACCAATCATTAACCTCCATCAGGGTTCAGGAGCCTCGTTCTGAGATTAAACAAGCTACTAGAGACTCGGGTTTGGCAACAAAAAAACGACGTCCCATTTGTTCTTGGGGTGTTATATGGAAGAAGAAGACTCCGGAAACTGGCACCGACTTCAGGATTAATAACATACTCCTCGGGGGTCGTTCGGATGTGCATGGGTTGAAACCTGTTTGCCACTTGTGCCATATGCCATATCTCTCCGATCTGACATATATACGCTGTGAATCTTGCAAAAGTAAGTTTGCTCCCATCAAAGTGTTGCCCCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCGTGTGTGTGTTTTTATTGGTGTTTAAGACTGTTTTGTGTTCATTCAGATTGGTACCATGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000122 GO:0000228 GO:0000785 GO:0000790 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0006139 GO:0006325 GO:0006338 GO:0006351 GO:0006355 GO:0006357 GO:0006725 GO:0006807 GO:0006996 GO:0007154 GO:0007165 GO:0007275 GO:0007399 GO:0007417 GO:0007420 GO:0008134 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009526 GO:0009527 GO:0009536 GO:0009707 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009941 GO:0009987 GO:0010019 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0016020 GO:0016043 GO:0016070 GO:0016589 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019867 GO:0023052 GO:0031010 GO:0031090 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031967 GO:0031968 GO:0031969 GO:0031974 GO:0031975 GO:0031981 GO:0032501 GO:0032502 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0035064 GO:0042170 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043565 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044454 GO:0044464 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0046483 GO:0048513 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051173 GO:0051252 GO:0051253 GO:0051254 GO:0051276 GO:0051716 GO:0060255 GO:0060322 GO:0065007 GO:0070013 GO:0070603 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:0098588 GO:0098805 GO:0140030 GO:0140034 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902494 GO:1902679 GO:1902680 GO:1903506 GO:1903507 GO:1903508 GO:1904949 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDT PHD WHIM1

Protein Analysis

1411

Amino Acids

157.75

Weight (kDa)

7.76

Isoelectric Point (pI)

43.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTM_DIR17_Tudor PF21743 40 - 85 5.1e-15 PTM/DIR17-like, Tudor domain
DDT PF02791 196 - 252 4.1e-18 DDT domain
WHIM1 PF15612 298 - 342 7.1e-10 WSTF, HB1, Itc1p, MBD9 motif 1
PHD PF00628 425 - 466 4.7e-08 PHD-finger
Chromo_PTM PF24294 1071 - 1143 2.9e-32 Membrane-bound transcription factor PTM, chromo domain-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3825
AatII GACGTC 2 cut(s) 754, 3919
Acc16I TGCGCA 1 cut(s) 3587
Acc36I ACCTGC 1 cut(s) 2687
Acc65I GGTACC 1 cut(s) 4225
AccB1I GGYRCC 2 cut(s) 3968, 4225
AccBSI CCGCTC 1 cut(s) 417
AccI GTMKAC 1 cut(s) 3551
AccIII TCCGGA 1 cut(s) 3958
AclWI GGATC 8 cut(s) 12, 1371, 1384, 1967, 2509, 3010, 3099, 3112
AcoI YGGCCR 4 cut(s) 32, 531, 1744, 2375
AcsI RAATTY 3 cut(s) 1198, 2635, 3012
AcuI CTGAAG 3 cut(s) 2130, 2277, 3961
AcyI GRCGYC 2 cut(s) 751, 3916
AfaI GTAC 7 cut(s) 125, 1495, 1879, 1892, 2253, 3637, 4227
AfiI CCNNNNNNNGG 6 cut(s) 563, 1763, 2748, 3846, 3965, 3978
AflII CTTAAG 1 cut(s) 1517
AflIII ACRYGT 1 cut(s) 126
AhlI ACTAGT 1 cut(s) 3749
AjiI CACGTC 1 cut(s) 2831
AjnI CCWGG 3 cut(s) 1379, 2041, 2286
AjuI GAANNNNNNNTTGG 4 cut(s) 74, 106, 1029, 1061
AloI GAACNNNNNNTCC 2 cut(s) 3659, 3691
Alw21I GWGCWC 8 cut(s) 201, 700, 1081, 1400, 1453, 1482, 2810, 2836
Alw26I GTCTC 6 cut(s) 89, 518, 742, 3326, 3528, 3882
Alw44I GTGCAC 4 cut(s) 197, 1396, 2806, 2832
AlwI GGATC 8 cut(s) 12, 1371, 1384, 1967, 2509, 3010, 3099, 3112
AlwNI CAGNNNCTG 1 cut(s) 3609
Ama87I CYCGRG 5 cut(s) 71, 2271, 3521, 3892, 3998
Aor13HI TCCGGA 1 cut(s) 3958
ApaI GGGCCC 1 cut(s) 71
ApaLI GTGCAC 4 cut(s) 197, 1396, 2806, 2832
ApeKI GCWGC 7 cut(s) 2225, 2234, 2237, 2987, 3080, 3197, 3484
ApoI RAATTY 3 cut(s) 1198, 2635, 3012
ArsI GACNNNNNNTTYG 4 cut(s) 2891, 2923, 3906, 3938
AseI ATTAAT 1 cut(s) 3984
Asp700I GAANNNNTTC 2 cut(s) 905, 2648
Asp718I GGTACC 1 cut(s) 4225
AspLEI GCGC 1 cut(s) 3588
AsuC2I CCSGG 5 cut(s) 511, 530, 545, 2305, 2743
AsuHPI GGTGA 5 cut(s) 248, 272, 470, 1883, 1951
AvaI CYCGRG 5 cut(s) 71, 2271, 3521, 3892, 3998
AvaII GGWCC 5 cut(s) 856, 1102, 1987, 2798, 3038
BaeGI GKGCMC 5 cut(s) 71, 201, 1400, 2810, 2836
BalI TGGCCA 2 cut(s) 1746, 2377
BamHI GGATCC 3 cut(s) 4, 1376, 3104
BanI GGYRCC 2 cut(s) 3968, 4225
BanII GRGCYC 1 cut(s) 71
BauI CACGAG 1 cut(s) 4172
BbsI GAAGAC 3 cut(s) 552, 1836, 3959
Bbv12I GWGCWC 8 cut(s) 201, 700, 1081, 1400, 1453, 1482, 2810, 2836
BbvI GCAGC 7 cut(s) 2212, 2221, 2224, 2999, 3067, 3184, 3496
BceAI ACGGC 1 cut(s) 518
BcgI CGANNNNNNTGC 2 cut(s) 2016, 2050
BciT130I CCWGG 3 cut(s) 1381, 2043, 2288
BclI TGATCA 2 cut(s) 3241, 3681
BcnI CCSGG 5 cut(s) 511, 530, 545, 2305, 2743
BcoDI GTCTC 6 cut(s) 89, 518, 742, 3326, 3528, 3882
BcuI ACTAGT 1 cut(s) 3749
BfaI CTAG 5 cut(s) 474, 1205, 2246, 3750, 3885
BfmI CTRYAG 3 cut(s) 2680, 3081, 3198
BfrI CTTAAG 1 cut(s) 1517
BfuAI ACCTGC 1 cut(s) 2687
BglI GCCNNNNNGGC 1 cut(s) 3630
BisI GCNGC 9 cut(s) 44, 539, 2226, 2235, 2238, 2988, 3081, 3198, 3485
BlsI GCNGC 9 cut(s) 45, 540, 2227, 2236, 2239, 2989, 3082, 3199, 3486
BmcAI AGTACT 1 cut(s) 1892
Bme1390I CCNGG 8 cut(s) 511, 530, 545, 1381, 2043, 2288, 2305, 2743
Bme18I GGWCC 5 cut(s) 856, 1102, 1987, 2798, 3038
BmeT110I CYCGRG 5 cut(s) 71, 2271, 3521, 3892, 3998
BmgBI CACGTC 1 cut(s) 2831
BmrFI CCNGG 8 cut(s) 511, 530, 545, 1381, 2043, 2288, 2305, 2743
BpiI GAAGAC 3 cut(s) 552, 1836, 3959
BpmI CTGGAG 1 cut(s) 2430
Bpu10I CCTNAGC 2 cut(s) 3171, 3447
BpuEI CTTGAG 2 cut(s) 766, 2732
BpuMI CCSGG 5 cut(s) 511, 530, 545, 2305, 2743
Bsa29I ATCGAT 1 cut(s) 104
BsaAI YACGTR 3 cut(s) 127, 2805, 3383
BsaBI GATNNNNATC 1 cut(s) 1321
BsaHI GRCGYC 2 cut(s) 751, 3916
BsaI GGTCTC 1 cut(s) 518
BsaWI WCCGGW 2 cut(s) 10, 3958
BsaXI ACNNNNNCTCC 2 cut(s) 271, 301
Bsc4I CCNNNNNNNGG 6 cut(s) 563, 1763, 2748, 3846, 3965, 3978
Bse118I RCCGGY 1 cut(s) 30
Bse3DI GCAATG 4 cut(s) 1155, 1298, 2902, 3595
Bse8I GATNNNNATC 1 cut(s) 1321
BseAI TCCGGA 1 cut(s) 3958
BseBI CCWGG 3 cut(s) 1381, 2043, 2288
BseCI ATCGAT 1 cut(s) 104
BseJI GATNNNNATC 1 cut(s) 1321
BseLI CCNNNNNNNGG 6 cut(s) 563, 1763, 2748, 3846, 3965, 3978
BseMI GCAATG 4 cut(s) 1155, 1298, 2902, 3595
BseMII CTCAG 7 cut(s) 795, 1383, 2013, 3083, 3162, 3461, 3858
BseRI GAGGAG 2 cut(s) 2606, 3986
BseSI GKGCMC 5 cut(s) 71, 201, 1400, 2810, 2836
BseX3I CGGCCG 2 cut(s) 32, 531
BseXI GCAGC 7 cut(s) 2212, 2221, 2224, 2999, 3067, 3184, 3496
BseYI CCCAGC 3 cut(s) 1802, 1962, 3108
BsgI GTGCAG 2 cut(s) 513, 3622
Bsh1285I CGRYCG 3 cut(s) 35, 534, 629
BshNI GGYRCC 2 cut(s) 3968, 4225
BshVI ATCGAT 1 cut(s) 104
BsiEI CGRYCG 3 cut(s) 35, 534, 629
BsiHKAI GWGCWC 8 cut(s) 201, 700, 1081, 1400, 1453, 1482, 2810, 2836
BsiHKCI CYCGRG 5 cut(s) 71, 2271, 3521, 3892, 3998
BsiSI CCGG 9 cut(s) 11, 31, 510, 530, 545, 581, 2305, 2742, 3959
BslFI GGGAC 3 cut(s) 1309, 3518, 3903
BslI CCNNNNNNNGG 6 cut(s) 563, 1763, 2748, 3846, 3965, 3978
BsmAI GTCTC 6 cut(s) 89, 518, 742, 3326, 3528, 3882
BsmBI CGTCTC 2 cut(s) 89, 742
BsmFI GGGAC 3 cut(s) 1309, 3518, 3903
BsmI GAATGC 1 cut(s) 249
Bso31I GGTCTC 1 cut(s) 518
BsoBI CYCGRG 5 cut(s) 71, 2271, 3521, 3892, 3998
Bsp120I GGGCCC 1 cut(s) 67
Bsp1286I GDGCHC 9 cut(s) 71, 201, 700, 1081, 1400, 1453, 1482, 2810, 2836
Bsp13I TCCGGA 1 cut(s) 3958
Bsp19I CCATGG 1 cut(s) 1867
BspCNI CTCAG 7 cut(s) 794, 1384, 2012, 3084, 3163, 3460, 3859
BspDI ATCGAT 1 cut(s) 104
BspEI TCCGGA 1 cut(s) 3958
BspMAI CTGCAG 3 cut(s) 2684, 3085, 3202
BspMI ACCTGC 1 cut(s) 2687
BspPI GGATC 8 cut(s) 12, 1371, 1384, 1967, 2509, 3010, 3099, 3112
BspT107I GGYRCC 2 cut(s) 3968, 4225
BspTI CTTAAG 1 cut(s) 1517
BspTNI GGTCTC 1 cut(s) 518
BsrBI CCGCTC 1 cut(s) 417
BsrDI GCAATG 4 cut(s) 1155, 1298, 2902, 3595
BsrFI RCCGGY 1 cut(s) 30
BssAI RCCGGY 1 cut(s) 30
BssNI GRCGYC 2 cut(s) 751, 3916
BssSI CACGAG 1 cut(s) 4172
BssT1I CCWWGG 2 cut(s) 1867, 2995
Bst2BI CACGAG 1 cut(s) 4172
Bst2UI CCWGG 3 cut(s) 1381, 2043, 2288
Bst6I CTCTTC 1 cut(s) 17
BstACI GRCGYC 2 cut(s) 751, 3916
BstAFI CTTAAG 1 cut(s) 1517
BstAPI GCANNNNNTGC 3 cut(s) 1076, 2234, 3696
BstBAI YACGTR 3 cut(s) 127, 2805, 3383
BstDSI CCRYGG 1 cut(s) 1867
BstENI CCTNNNNNAGG 1 cut(s) 3844
BstHHI GCGC 1 cut(s) 3588
BstMAI GTCTC 6 cut(s) 89, 518, 742, 3326, 3528, 3882
BstMCI CGRYCG 3 cut(s) 35, 534, 629
BstNI CCWGG 3 cut(s) 1381, 2043, 2288
BstNSI RCATGY 5 cut(s) 1030, 1134, 2037, 2075, 2473
BstSCI CCNGG 8 cut(s) 509, 528, 543, 1379, 2041, 2286, 2303, 2741
BstSFI CTRYAG 3 cut(s) 2680, 3081, 3198
BstSLI GKGCMC 5 cut(s) 71, 201, 1400, 2810, 2836
BstV1I GCAGC 7 cut(s) 2212, 2221, 2224, 2999, 3067, 3184, 3496
BstV2I GAAGAC 3 cut(s) 552, 1836, 3959
BstX2I RGATCY 4 cut(s) 4, 1376, 2514, 3104
BstYI RGATCY 4 cut(s) 4, 1376, 2514, 3104
BstZI CGGCCG 2 cut(s) 32, 531
Bsu15I ATCGAT 1 cut(s) 104
BsuTUI ATCGAT 1 cut(s) 104
BtgI CCRYGG 1 cut(s) 1867
BtgZI GCGATG 1 cut(s) 2551
BtrI CACGTC 1 cut(s) 2831
BtsI GCAGTG 3 cut(s) 593, 2689, 3610
BtsIMutI CAGTG 8 cut(s) 593, 840, 1081, 2062, 2689, 2808, 3610, 3788
BveI ACCTGC 1 cut(s) 2687
CaiI CAGNNNCTG 1 cut(s) 3609
CfoI GCGC 1 cut(s) 3588
Cfr10I RCCGGY 1 cut(s) 30
ClaI ATCGAT 1 cut(s) 104
Csp6I GTAC 7 cut(s) 124, 1494, 1878, 1891, 2252, 3636, 4226
CspCI CAANNNNNGTGG 2 cut(s) 2131, 2166
CviQI GTAC 7 cut(s) 124, 1494, 1878, 1891, 2252, 3636, 4226
DraI TTTAAA 1 cut(s) 2182
EaeI YGGCCR 4 cut(s) 32, 531, 1744, 2375
EagI CGGCCG 2 cut(s) 32, 531
Eam1104I CTCTTC 1 cut(s) 17
EarI CTCTTC 1 cut(s) 17
EciI GGCGGA 2 cut(s) 2642, 2863
EclXI CGGCCG 2 cut(s) 32, 531
Eco130I CCWWGG 2 cut(s) 1867, 2995
Eco147I AGGCCT 1 cut(s) 3814
Eco24I GRGCYC 1 cut(s) 71
Eco31I GGTCTC 1 cut(s) 518
Eco47I GGWCC 5 cut(s) 856, 1102, 1987, 2798, 3038
Eco52I CGGCCG 2 cut(s) 32, 531
Eco57I CTGAAG 3 cut(s) 2130, 2277, 3961
Eco88I CYCGRG 5 cut(s) 71, 2271, 3521, 3892, 3998
EcoNI CCTNNNNNAGG 1 cut(s) 3844
EcoO109I RGGNCCY 2 cut(s) 68, 2798
EcoRII CCWGG 3 cut(s) 1379, 2041, 2286
EcoT14I CCWWGG 2 cut(s) 1867, 2995
EcoT22I ATGCAT 2 cut(s) 2194, 2471
EcoT38I GRGCYC 1 cut(s) 71
ErhI CCWWGG 2 cut(s) 1867, 2995
Esp3I CGTCTC 2 cut(s) 89, 742
FaqI GGGAC 3 cut(s) 1309, 3518, 3903
FauI CCCGC 3 cut(s) 39, 58, 3506
FauNDI CATATG 1 cut(s) 4050
FbaI TGATCA 2 cut(s) 3241, 3681
FblI GTMKAC 1 cut(s) 3551
Fnu4HI GCNGC 9 cut(s) 44, 539, 2226, 2235, 2238, 2988, 3081, 3198, 3485
FriOI GRGCYC 1 cut(s) 71
FseI GGCCGGCC 1 cut(s) 34
Fsp4HI GCNGC 9 cut(s) 44, 539, 2226, 2235, 2238, 2988, 3081, 3198, 3485
FspBI CTAG 5 cut(s) 474, 1205, 2246, 3750, 3885
FspI TGCGCA 1 cut(s) 3587
GlaI GCGC 1 cut(s) 3587
GluI GCNGC 9 cut(s) 44, 539, 2226, 2235, 2238, 2988, 3081, 3198, 3485
GsaI CCCAGC 3 cut(s) 1806, 1966, 3112
GsuI CTGGAG 1 cut(s) 2430
HapII CCGG 9 cut(s) 11, 31, 510, 530, 545, 581, 2305, 2742, 3959
HhaI GCGC 1 cut(s) 3588
Hin1I GRCGYC 2 cut(s) 751, 3916
Hin6I GCGC 1 cut(s) 3586
HinP1I GCGC 1 cut(s) 3586
HincII GTYRAC 3 cut(s) 2005, 2053, 3569
HindII GTYRAC 3 cut(s) 2005, 2053, 3569
HindIII AAGCTT 2 cut(s) 2393, 3210
HpaII CCGG 9 cut(s) 11, 31, 510, 530, 545, 581, 2305, 2742, 3959
HphI GGTGA 5 cut(s) 248, 272, 470, 1883, 1951
Hpy99I CGWCG 1 cut(s) 3918
HpyCH4IV ACGT 8 cut(s) 126, 751, 2049, 2365, 2804, 2830, 3382, 3916
HpySE526I ACGT 8 cut(s) 126, 751, 2049, 2365, 2804, 2830, 3382, 3916
Hsp92I GRCGYC 2 cut(s) 751, 3916
HspAI GCGC 1 cut(s) 3586
Kpn2I TCCGGA 1 cut(s) 3958
KpnI GGTACC 1 cut(s) 4229
KroI GCCGGC 1 cut(s) 30
KroNI GCCGGC 1 cut(s) 32
Ksp22I TGATCA 2 cut(s) 3241, 3681
Lsp1109I GCAGC 7 cut(s) 2212, 2221, 2224, 2999, 3067, 3184, 3496
MaeI CTAG 5 cut(s) 474, 1205, 2246, 3750, 3885
MaeII ACGT 8 cut(s) 126, 751, 2049, 2365, 2804, 2830, 3382, 3916
MbiI CCGCTC 1 cut(s) 417
MfeI CAATTG 3 cut(s) 1454, 2019, 2730
MflI RGATCY 4 cut(s) 4, 1376, 2514, 3104
MhlI GDGCHC 9 cut(s) 71, 201, 700, 1081, 1400, 1453, 1482, 2810, 2836
MlsI TGGCCA 2 cut(s) 1746, 2377
MluNI TGGCCA 2 cut(s) 1746, 2377
MlyI GAGTC 3 cut(s) 2605, 3884, 3949
MmeI TCCRAC 4 cut(s) 1027, 1411, 2794, 3770
Mox20I TGGCCA 2 cut(s) 1746, 2377
Mph1103I ATGCAT 2 cut(s) 2194, 2471
MroI TCCGGA 1 cut(s) 3958
MroNI GCCGGC 1 cut(s) 30
MroXI GAANNNNTTC 2 cut(s) 905, 2648
MscI TGGCCA 2 cut(s) 1746, 2377
MslI CAYNNNNRTG 7 cut(s) 254, 575, 796, 1487, 1540, 2076, 4118
Msp20I TGGCCA 2 cut(s) 1746, 2377
MspA1I CMGCKG 1 cut(s) 3487
MspCI CTTAAG 1 cut(s) 1517
MspI CCGG 9 cut(s) 11, 31, 510, 530, 545, 581, 2305, 2742, 3959
MspR9I CCNGG 8 cut(s) 511, 530, 545, 1381, 2043, 2288, 2305, 2743
MunI CAATTG 3 cut(s) 1454, 2019, 2730
Mva1269I GAATGC 1 cut(s) 249
MvaI CCWGG 3 cut(s) 1381, 2043, 2288
NaeI GCCGGC 1 cut(s) 32
NciI CCSGG 5 cut(s) 511, 530, 545, 2305, 2743
NcoI CCATGG 1 cut(s) 1867
NdeI CATATG 1 cut(s) 4050
NgoMIV GCCGGC 1 cut(s) 30
NmeAIII GCCGAG 1 cut(s) 60
NmuCI GTSAC 5 cut(s) 329, 835, 1749, 1957, 3796
NsbI TGCGCA 1 cut(s) 3587
NsiI ATGCAT 2 cut(s) 2194, 2471
NspI RCATGY 5 cut(s) 1030, 1134, 2037, 2075, 2473
PaeI GCATGC 3 cut(s) 1134, 2075, 2473
PaeR7I CTCGAG 1 cut(s) 3521
PceI AGGCCT 1 cut(s) 3814
PctI GAATGC 1 cut(s) 249
PdiI GCCGGC 1 cut(s) 32
PdmI GAANNNNTTC 2 cut(s) 905, 2648
PkrI GCNGC 9 cut(s) 45, 540, 2227, 2236, 2239, 2989, 3082, 3199, 3486
PleI GAGTC 3 cut(s) 2604, 3884, 3949
PpsI GAGTC 3 cut(s) 2604, 3884, 3949
Ppu21I YACGTR 3 cut(s) 127, 2805, 3383
PpuMI RGGWCCY 1 cut(s) 2798
PshBI ATTAAT 1 cut(s) 3984
PsiI TTATAA 1 cut(s) 3825
Psp5II RGGWCCY 1 cut(s) 2798
Psp6I CCWGG 3 cut(s) 1379, 2041, 2286
PspFI CCCAGC 3 cut(s) 1802, 1962, 3108
PspGI CCWGG 3 cut(s) 1379, 2041, 2286
PspOMI GGGCCC 1 cut(s) 67
PspPPI RGGWCCY 1 cut(s) 2798
PsrI GAACNNNNNNTAC 2 cut(s) 3628, 3660
PstI CTGCAG 3 cut(s) 2684, 3085, 3202
PstNI CAGNNNCTG 1 cut(s) 3609
PsuI RGATCY 4 cut(s) 4, 1376, 2514, 3104
RigI GGCCGGCC 1 cut(s) 34
RsaI GTAC 7 cut(s) 125, 1495, 1879, 1892, 2253, 3637, 4227
RsaNI GTAC 7 cut(s) 124, 1494, 1878, 1891, 2252, 3636, 4226
RseI CAYNNNNRTG 7 cut(s) 254, 575, 796, 1487, 1540, 2076, 4118
SatI GCNGC 9 cut(s) 44, 539, 2226, 2235, 2238, 2988, 3081, 3198, 3485
ScaI AGTACT 1 cut(s) 1892
SchI GAGTC 3 cut(s) 2605, 3884, 3949
ScrFI CCNGG 8 cut(s) 511, 530, 545, 1381, 2043, 2288, 2305, 2743
SduI GDGCHC 9 cut(s) 71, 201, 700, 1081, 1400, 1453, 1482, 2810, 2836
SfcI CTRYAG 3 cut(s) 2680, 3081, 3198
Sfr274I CTCGAG 1 cut(s) 3521
SinI GGWCC 5 cut(s) 856, 1102, 1987, 2798, 3038
SlaI CTCGAG 1 cut(s) 3521
SmiMI CAYNNNNRTG 7 cut(s) 254, 575, 796, 1487, 1540, 2076, 4118
SmlI CTYRAG 4 cut(s) 745, 1517, 2711, 3521
SmoI CTYRAG 4 cut(s) 745, 1517, 2711, 3521
SpeI ACTAGT 1 cut(s) 3749
SphI GCATGC 3 cut(s) 1134, 2075, 2473
SseBI AGGCCT 1 cut(s) 3814
SspI AATATT 1 cut(s) 1652
SspMI CTAG 5 cut(s) 474, 1205, 2246, 3750, 3885
StuI AGGCCT 1 cut(s) 3814
StyD4I CCNGG 8 cut(s) 509, 528, 543, 1379, 2041, 2286, 2303, 2741
StyI CCWWGG 2 cut(s) 1867, 2995
TaiI ACGT 8 cut(s) 129, 754, 2052, 2368, 2807, 2833, 3385, 3919
TaqII GACCGA 1 cut(s) 2040
TatI WGTACW 3 cut(s) 1877, 1890, 2251
TauI GCSGC 2 cut(s) 46, 541
TscAI CASTG 8 cut(s) 593, 840, 1081, 2062, 2689, 2815, 3610, 3795
TseFI GTSAC 5 cut(s) 329, 835, 1749, 1957, 3796
TseI GCWGC 7 cut(s) 2225, 2234, 2237, 2987, 3080, 3197, 3484
Tsp45I GTSAC 5 cut(s) 329, 835, 1749, 1957, 3796
TspGWI ACGGA 1 cut(s) 3117
TspRI CASTG 8 cut(s) 593, 840, 1081, 2062, 2689, 2815, 3610, 3795
Vha464I CTTAAG 1 cut(s) 1517
VneI GTGCAC 4 cut(s) 197, 1396, 2806, 2832
VpaK11BI GGWCC 5 cut(s) 856, 1102, 1987, 2798, 3038
VspI ATTAAT 1 cut(s) 3984
XagI CCTNNNNNAGG 1 cut(s) 3844
XapI RAATTY 3 cut(s) 1198, 2635, 3012
XceI RCATGY 5 cut(s) 1030, 1134, 2037, 2075, 2473
XcmI CCANNNNNNNNNTGG 1 cut(s) 1377
XhoI CTCGAG 1 cut(s) 3521
XmiI GTMKAC 1 cut(s) 3551
XmnI GAANNNNTTC 2 cut(s) 905, 2648
XspI CTAG 5 cut(s) 474, 1205, 2246, 3750, 3885
ZraI GACGTC 2 cut(s) 752, 3917
ZrmI AGTACT 1 cut(s) 1892
Zsp2I ATGCAT 2 cut(s) 2194, 2471
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.