Rroxscaffold_1G00024660

WSTF, HB1, Itc1p, MBD9 motif 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0007748
Physical Location & Seq
Reverse (-)
30656382 .. 30664538
8157 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00024660.1

Sequence Viewer

Length: 5043 bp
ATGGATCCTCCGGTGGCGAAAAAGAGGGGCCGGCCGAGGAAGCGGCGGGCTGATGATCGGAAAGCGGGGCCCGAGGCGAAGAAACAGGCATTGGAGACGAGATCGATTGCGTTGTTGGGACGGTACGTGTTGAAGGAGTTTGAAAGTAGGATTTATCTGGGGAAAGTTGCGTTTTATGATGCTGGATTGTATAGAGTGCACTATGAAGATGGGGATTTCGAGGACTTGGAGAGTGGCGAGATTCGGGGCATTCTTGTGGGTGAGAGTGATTTTGATGGGAGTTTGAGTGCTAAGAGGAAGAAATTGGATAAGCTGGTGGCGAAAGTGAGTGACGAAAAGAAGGTTAAGGTGGAGGAGAATGTGGAAAAGGAGGTGGTGGATAGGGTTGAAACCTCGGAATTGAGTGAGTGGAGCGGTAGTGTAACTATTGACAATGATGAGATTCGAGAGGATGGTGATGCTGATTTGTCTAGTGATTCGAGTGAGTGTGCAGGGGATATGGATGCCGGGGTTGATGTTGAGACCCCAGCTGTGCCGCCGCCGGAGTTGCCACCGTCTTCTGGAACCATAGGGGTGCCGGAGCAGTGTGTTTCGCATCTTTTCTCTGTATATGGTTTCATGCGGTCGTTTAGCATCTGCCTGTTCTTGAACCCGTTTACTTTGGATGATTTTGTGGGTTCGCTTAATTGTCGTGCTCCAAACACATTGTTTGATGCAATTCATGTTGCTTTGTTGCGTGCCTTGAGACGTCATCTTGAAACAATCTCATCCGAAGGCTCAGAGGTAGCACAAAAATGTTTGAGGTGCATAGATTGGAGCTTGCTCGATACAGTGACTTGGCCTGTTTACTTGGTCCAGTATATAACAATTATGGGATATGTAAAAGGACAAGAGTGGAAAGGTTTCTATGATGAGGTTTTGGACAGAGAGTATTATTTATTATCAGTTGATAGGAAGTTAAGTATTTTGCAAATCATTTGTGATGATGTCTTAGACAGTACAGATATAAGAGCTGAATTAGACATGCGTGAAGAATCAGAAGTTGGAATAGATTATGATGCCGAAGCAACCGGTGCTCTTGTAAATGGGCCAAGAAGGGTCCATCCGAGATATTCTAAAACTTCTGCATGCAAGGACAGAGAAGCAATGGAAATTGCTACTGAATCCCATGATATAAAATTATCTGGTAACTTAAATTCACTAGGTTCCAAAGTTGCTAAAGGGGAGTTTGATGCTACTGATGTTGATTCGGATTGTAATTCTGATGAATGTCGGCTTTGTGGTATGGATGGGACATTGCTTTGCTGTGATGGATGCCCATCTGCCTACCATACGAGGTGTATAGGTGTGATGAAACTGTCGATACCAGAAGGATCCTGGTATTGTCCTGAGTGCACTATCAACAAAATTGGGCCAACTATCACAGTTGGAACATCAGTTAAAGGAGCACAATTGTTTGGGATAGATACCTACGAGCACATCTTCATGGGTACTTGCAACCACTTACTTGTGCTTAAGGAAACTATCAACACACAACCATGTCTTAGATATTACAATCAGCATGACATTCCAAAAGTCCTGAAAGTTCTTTGTTCATCTGGGCAAAATACATCTTTTTATCTGGGCTTGTGCAAGGCAATTTTAGAATACTGGAATATCCCAGAAAGCATCTTATCTCTTTCAGAGCTGGCTGAATCAATGACTAATTTGGTGAAGATAAAGGAAGATGCGAACTTTTGTGGCCAGTCACTTCCTTTGTCTGGCAAGGATTGTGATAAAGTTATGGATATGGTTGAGGCTGGGAACTGTGAAACTTATGTAAATGAAGACCATTTAGTTAAAGCAGTTTCATCTTTCGATTGCCAAGGTGATAGTACACCACAGGCGTACTCACATAGGAATATGGAGATTGATGAAAGGAGTAAAATGGAATATGCTGTTTCGACTGGTTCTGTCACCCAGCAAGCTGATCCATCTTACTTGGTCCACAGAAGCTCAGTTGACAGATCAACTGCAATTGACCGAAGCACATGCACACCAGGGAACGTCAACAGTGGTAACAAAGTGCATGCAAATGGTATGCGTCTTTCAGTGACTTCACAAAATGAAGAAGGCAATCATATAGGAAAGGTTTACTCCACTTCAGTTGATGATTGCAAATACAAGGGTTCTTTCTTTAAACCCCATGCATACATAAATTACTATGTGCATGGGGATTTTGCTGCATCTGCTGCTGCCAAACTGGCTGTACTTTCATCAGAAGAAGCTCGGGGTTCAGATGTCCAGGCTTCAGGCAATCCCCGGAAAGTTGCATCATCAAACAATTTATGGCAAGCAAAAGCCTTCTCACTAACAGCCTCACGTTTCTTCTGGCCAAGTTTTGAAAAGAAGCTTGTGGAGGTTCCAAGAGAGAGGTGTGGTTGGTGTCTTTCTTGTAAGGCTCCAGTTGCAAGCAAGAGAGGATGCATGCTAAATCATGCTTGTTTGAGTGCCACTAAAGGTGCTATGAAGATCCTTGCCAGCCTTCGTCCTATAAAGAGTGTGGAGGGGAATCTTGTAAGCATCGCAGCATACATCTTATACATGGAGGAGAGTTTGCATGGTCTAATAACTGGCCCCTTTCTCAATGAAAATTTTAGGAAACAATTCCGCCAACAAGTTTATCAAGCATCAACCTGCAGTGCAATAAAAGTCCTATTGCTCAAACTTGAGGAAAACATCCGAACAATTGCTCTTTCCGGGGAGTGGATTAAGCTGGTAGATGATTTGCTGGTTGAATCTTCTGTGATTCAAGGTCCTACGTGCACTGTTGGAACAACACAAAGACGTGCGCCATACAGCAGGCGGAGCAGGAAACAGAGTGCAATTCAAGAAGTTATAGATGATGACTGCAATGACAAGAGTTTTGTATGGTGGCAAGGAGGGAAGCTGTCAAAGATCATATTCCAGAGAGCAATCTTGGCTCGCTCATTGGTTAAAAAAGCAGCTCGCCAAGGTGGATCGAGGAAAATTTTTGGTGTATCCTACGCTGATGGTCCTGATATTCCTAAAAGAAGCAGACAATCTGTATGGAGGGCTGCAGTTGAACTGAGCAAGAACGGATCCCAGCTTGCAGTTCAGGTTAGATACTTGGACTATCATTTGAGGTGGAGTGATCTTGTTCGTCCTGAGCAGAACCTCCTTGATGGGAAAGCTGCAGAGGCAGAAGCTTCTGCTTTTAGAAATGCAAGTATATGTGATAAAAAAATGTTAAAGAACAACATCGTCTATGGGGTTGCATTTGGTAGTCAAAAGCATCTGCCTAACCGTGTAATGAAAAATATCATTGAGACAGAGCAGAATCAGGATGGAACTAACAAGTTCTGGTTTCTTGAATCACGTATTCCTTTGTATTTGATTAAGGAATATGAAGAAAGTGTAGGTAAAGTGTCTATTCCATCGGCTCAGGAGCCTAATCTATTGCACAAGTTACAGAGAAGGCAGCGGAAAGCAACCCGCAGGGACATATTTTTTTATCTCGAGTGTAAAAGAGACAATCTGGACTTGTCTACCTGCTCTATGTGTCAACTGGATATTTTATCGCGCAATGCAGTCAAGTGCAGTACCTGTCAAGGTTATTGCCATGAGGGCTGTACCATAAGTTCAACAGTTTCTACAAATGAGGAAGTTGAGTTCTTGATCACTTGCAAGCGGTGCTACCATGCGAAAGCTCTTGCCCAGAAGCAAAAGTTTAAGGAGTTTCCAACTAGTCCTTTGCCCTTGCAAATGAAAGAATATCATACTCCACTGACAGTCACAACTGGTGGAAGGCCTAAAAATTATAACCAATCATTAACCTCCATCAGGGTTCAGGAGCCTCGTTCTGAGATTAAACAAGCTACTACAGACTCGGGTTTGGCAACAAAAAAACGACGTCCCATTTGTTCTTGGGGTGTTATATGGAAGAAGAAGACTCCGGAAACTGGCACCGACTTCAGGATTAATAACATACTCGGGGGTCGTTCGGATGTGCATGGGTTGAAACCTGTTTGCCACTTGTGCCATATGCCATATCTCTCTGATCTGACATATATACGCTGTGAATCTTGCAAAAATTGGTACCATGCTGAAGCTGTCGAACTTGAGGAGTCAAAGATCTGTGAAGTGGCAGGCTTCAAGTGTTGCAAGTGTCGCAGGATAAAATCGCCTCTTTGTCCTTATACTGATCTTAAAGACAAGACCTTGCAAGAGAGCAAGAAGATTCGCAATAAGCGTTCAAAGCAAGAAAATCTTGGAGAGGACTCGGATTCTGCAACCAATTTTGATTCAGAGTTGTTTGAACCTGCTACTCCAGTGTTTCCCATGGAGGAAGTCATCATTCAGGATGATGATCCTCTTCTTTTTGCTCTCTCAAGAGTGGAGCTAGTTCCAGAACACAATTCAGAAGTTAATGCTGAATGGGATACTGCTGGGCCAGGGCCACGAAAATTACCTGTGAGAAGGCATGTTAAGCGTGAAGAGGACTTGGATATATATTGTGACAGTAATAAATCTCATGCTGAAAAAACTATGCATGATGAAACAAATTACCTGTCGGAACCGATGGAGATTGCGGAATTCCCTCATGTGGAATGGGATACTTCCATGAACGGCATCAATGGTGAGATAATGGAACAGTATGAAGATCTGAACTACGATATGATGGAACCGCAAACTGTCTTTACTATCAATGAGTTGCTGGCACCTGATGACGGCGACCTTTTCGATGGAGTCGAAACATTTGCGGATTTACCAGAAAATCTGGAAAATCCATATACAACTTCACAGCATGTAGGCCCTGAGCAATATGATATGGCCACTTTCACAGACGAATCAAAGTCTGCAATTCCTGAAACATCTGCTGTTAATATGATGCAATGTCAGATATGTTTACATGCAGAACCAGCTCCTGATCTTTCTTGCCAGAACTGTGGGCTATTAATTCATAACCGTTGTTCCCCTTGGATTGAGTCATCATCTCAGAATAATAGCTGGAAGTGTGGACAGTGCCGGGAGTGGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000122 GO:0000228 GO:0000785 GO:0000790 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0006139 GO:0006325 GO:0006338 GO:0006351 GO:0006355 GO:0006357 GO:0006725 GO:0006807 GO:0006996 GO:0007154 GO:0007165 GO:0007275 GO:0007399 GO:0007417 GO:0007420 GO:0008134 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009526 GO:0009527 GO:0009536 GO:0009707 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009941 GO:0009987 GO:0010019 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0016020 GO:0016043 GO:0016070 GO:0016589 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019867 GO:0023052 GO:0031010 GO:0031090 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031967 GO:0031968 GO:0031969 GO:0031974 GO:0031975 GO:0031981 GO:0032501 GO:0032502 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0035064 GO:0042170 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043565 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044454 GO:0044464 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0046483 GO:0048513 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051173 GO:0051252 GO:0051253 GO:0051254 GO:0051276 GO:0051716 GO:0060255 GO:0060322 GO:0065007 GO:0070013 GO:0070603 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:0098588 GO:0098805 GO:0140030 GO:0140034 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902494 GO:1902679 GO:1902680 GO:1903506 GO:1903507 GO:1903508 GO:1904949 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDT PHD WHIM1

Protein Analysis

1680

Amino Acids

188.55

Weight (kDa)

5.71

Isoelectric Point (pI)

45.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTM_DIR17_Tudor PF21743 40 - 85 6.2e-15 PTM/DIR17-like, Tudor domain
DDT PF02791 195 - 251 5e-18 DDT domain
WHIM1 PF15612 297 - 341 2.7e-10 WSTF, HB1, Itc1p, MBD9 motif 1
PHD PF00628 424 - 465 5.7e-08 PHD-finger
Chromo_PTM PF24294 1070 - 1142 4.3e-33 Membrane-bound transcription factor PTM, chromo domain-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3822
AatII GACGTC 2 cut(s) 751, 3916
Acc36I ACCTGC 3 cut(s) 2684, 3560, 4330
Acc65I GGTACC 1 cut(s) 4098
AccB1I GGYRCC 4 cut(s) 574, 3965, 4098, 4720
AccBSI CCGCTC 1 cut(s) 414
AccI GTMKAC 1 cut(s) 3548
AccII CGCG 1 cut(s) 3583
AccIII TCCGGA 1 cut(s) 3955
AclWI GGATC 9 cut(s) 12, 1368, 1381, 1964, 2506, 3007, 3096, 3109, 4364
AcoI YGGCCR 4 cut(s) 32, 1741, 2372, 4833
AcsI RAATTY 4 cut(s) 1195, 2632, 3009, 4595
AcuI CTGAAG 4 cut(s) 2127, 2274, 3958, 4128
AcyI GRCGYC 2 cut(s) 748, 3913
AfaI GTAC 9 cut(s) 125, 1000, 1492, 1876, 1889, 2250, 3604, 3634, 4100
AfiI CCNNNNNNNGG 8 cut(s) 560, 1760, 2745, 3843, 3962, 3975, 4606, 4730
AflII CTTAAG 1 cut(s) 1514
AflIII ACRYGT 1 cut(s) 126
AgeI ACCGGT 1 cut(s) 1070
AhlI ACTAGT 1 cut(s) 3746
AjiI CACGTC 1 cut(s) 2828
AjnI CCWGG 4 cut(s) 1376, 2038, 2283, 4453
AjuI GAANNNNNNNTTGG 4 cut(s) 74, 106, 1026, 1058
AloI GAACNNNNNNTCC 2 cut(s) 3656, 3688
Alw21I GWGCWC 7 cut(s) 201, 697, 1078, 1397, 1450, 1479, 2807
Alw26I GTCTC 5 cut(s) 89, 515, 739, 3323, 3525
Alw44I GTGCAC 3 cut(s) 197, 1393, 2803
AlwI GGATC 9 cut(s) 12, 1368, 1381, 1964, 2506, 3007, 3096, 3109, 4364
AlwNI CAGNNNCTG 2 cut(s) 3606, 4928
Ama87I CYCGRG 5 cut(s) 71, 2268, 3518, 3889, 3992
Aor13HI TCCGGA 1 cut(s) 3955
ApaI GGGCCC 1 cut(s) 72
ApaLI GTGCAC 3 cut(s) 197, 1393, 2803
ApeKI GCWGC 8 cut(s) 2222, 2231, 2234, 2567, 2984, 3077, 3194, 3481
ApoI RAATTY 4 cut(s) 1195, 2632, 3009, 4595
ArsI GACNNNNNNTTYG 6 cut(s) 2888, 2920, 3903, 3935, 4556, 4588
AseI ATTAAT 2 cut(s) 3981, 4958
AsiGI ACCGGT 1 cut(s) 1070
Asp700I GAANNNNTTC 2 cut(s) 902, 2645
Asp718I GGTACC 1 cut(s) 4098
AspLEI GCGC 2 cut(s) 2833, 3585
AsuC2I CCSGG 4 cut(s) 508, 2302, 2740, 5030
AsuHPI GGTGA 6 cut(s) 272, 467, 1723, 1880, 1948, 4652
AvaI CYCGRG 5 cut(s) 71, 2268, 3518, 3889, 3992
AvaII GGWCC 5 cut(s) 853, 1099, 1984, 2795, 3035
BaeGI GKGCMC 4 cut(s) 72, 201, 1397, 2807
BalI TGGCCA 3 cut(s) 1743, 2374, 4835
BamHI GGATCC 3 cut(s) 4, 1373, 3101
BanI GGYRCC 4 cut(s) 574, 3965, 4098, 4720
BanII GRGCYC 1 cut(s) 72
BbsI GAAGAC 3 cut(s) 549, 1833, 3956
Bbv12I GWGCWC 7 cut(s) 201, 697, 1078, 1397, 1450, 1479, 2807
BbvI GCAGC 8 cut(s) 2209, 2218, 2221, 2579, 2996, 3064, 3181, 3493
BceAI ACGGC 2 cut(s) 4645, 4747
BcgI CGANNNNNNTGC 4 cut(s) 2013, 2047, 4742, 4776
BciT130I CCWGG 4 cut(s) 1378, 2040, 2285, 4455
BciVI GTATCC 3 cut(s) 3031, 4435, 4609
BclI TGATCA 1 cut(s) 3678
BcnI CCSGG 4 cut(s) 508, 2302, 2740, 5030
BcoDI GTCTC 5 cut(s) 89, 515, 739, 3323, 3525
BcuI ACTAGT 1 cut(s) 3746
BfaI CTAG 4 cut(s) 471, 1202, 3747, 4403
BfmI CTRYAG 4 cut(s) 2677, 3078, 3195, 3882
BfrI CTTAAG 1 cut(s) 1514
BfuAI ACCTGC 3 cut(s) 2684, 3560, 4330
BfuI GTATCC 3 cut(s) 3031, 4435, 4609
BglI GCCNNNNNGGC 2 cut(s) 2243, 3627
BglII AGATCT 2 cut(s) 4134, 4663
Bme1390I CCNGG 8 cut(s) 508, 1378, 2040, 2285, 2302, 2740, 4455, 5030
Bme18I GGWCC 5 cut(s) 853, 1099, 1984, 2795, 3035
BmeT110I CYCGRG 5 cut(s) 71, 2268, 3518, 3889, 3992
BmgBI CACGTC 1 cut(s) 2828
BmrFI CCNGG 8 cut(s) 508, 1378, 2040, 2285, 2302, 2740, 4455, 5030
BpiI GAAGAC 3 cut(s) 549, 1833, 3956
BpmI CTGGAG 2 cut(s) 2427, 4314
Bpu10I CCTNAGC 3 cut(s) 3168, 3444, 4818
BpuEI CTTGAG 4 cut(s) 763, 2729, 4142, 4375
BpuMI CCSGG 4 cut(s) 508, 2302, 2740, 5030
Bsa29I ATCGAT 1 cut(s) 104
BsaAI YACGTR 3 cut(s) 127, 2802, 3380
BsaBI GATNNNNATC 2 cut(s) 1318, 4368
BsaHI GRCGYC 2 cut(s) 748, 3913
BsaI GGTCTC 1 cut(s) 515
BsaWI WCCGGW 3 cut(s) 10, 1070, 3955
BsaXI ACNNNNNCTCC 5 cut(s) 271, 301, 403, 433, 5024
Bsc4I CCNNNNNNNGG 8 cut(s) 560, 1760, 2745, 3843, 3962, 3975, 4606, 4730
Bse118I RCCGGY 2 cut(s) 30, 1070
Bse3DI GCAATG 5 cut(s) 1152, 1295, 2899, 3592, 4901
Bse8I GATNNNNATC 2 cut(s) 1318, 4368
BseAI TCCGGA 1 cut(s) 3955
BseBI CCWGG 4 cut(s) 1378, 2040, 2285, 4455
BseCI ATCGAT 1 cut(s) 104
BseJI GATNNNNATC 2 cut(s) 1318, 4368
BseLI CCNNNNNNNGG 8 cut(s) 560, 1760, 2745, 3843, 3962, 3975, 4606, 4730
BseMI GCAATG 5 cut(s) 1152, 1295, 2899, 3592, 4901
BseMII CTCAG 9 cut(s) 792, 1380, 2010, 3080, 3159, 3458, 3855, 4809, 5012
BseRI GAGGAG 3 cut(s) 368, 2603, 4139
BseSI GKGCMC 4 cut(s) 72, 201, 1397, 2807
BseX3I CGGCCG 1 cut(s) 32
BseXI GCAGC 8 cut(s) 2209, 2218, 2221, 2579, 2996, 3064, 3181, 3493
BseYI CCCAGC 5 cut(s) 526, 1799, 1959, 3105, 4448
BsgI GTGCAG 2 cut(s) 510, 3619
Bsh1236I CGCG 1 cut(s) 3583
Bsh1285I CGRYCG 2 cut(s) 35, 626
BshNI GGYRCC 4 cut(s) 574, 3965, 4098, 4720
BshTI ACCGGT 1 cut(s) 1070
BshVI ATCGAT 1 cut(s) 104
BsiEI CGRYCG 2 cut(s) 35, 626
BsiHKAI GWGCWC 7 cut(s) 201, 697, 1078, 1397, 1450, 1479, 2807
BsiHKCI CYCGRG 5 cut(s) 71, 2268, 3518, 3889, 3992
BslFI GGGAC 4 cut(s) 132, 1306, 3515, 3900
BslI CCNNNNNNNGG 8 cut(s) 560, 1760, 2745, 3843, 3962, 3975, 4606, 4730
BsmAI GTCTC 5 cut(s) 89, 515, 739, 3323, 3525
BsmBI CGTCTC 2 cut(s) 89, 739
BsmFI GGGAC 4 cut(s) 132, 1306, 3515, 3900
BsmI GAATGC 1 cut(s) 249
Bso31I GGTCTC 1 cut(s) 515
BsoBI CYCGRG 5 cut(s) 71, 2268, 3518, 3889, 3992
Bsp120I GGGCCC 1 cut(s) 68
Bsp1286I GDGCHC 8 cut(s) 72, 201, 697, 1078, 1397, 1450, 1479, 2807
Bsp13I TCCGGA 1 cut(s) 3955
Bsp19I CCATGG 1 cut(s) 4341
BspCNI CTCAG 9 cut(s) 791, 1381, 2009, 3081, 3160, 3457, 3856, 4810, 5011
BspDI ATCGAT 1 cut(s) 104
BspEI TCCGGA 1 cut(s) 3955
BspFNI CGCG 1 cut(s) 3583
BspMAI CTGCAG 3 cut(s) 2681, 3082, 3199
BspMI ACCTGC 3 cut(s) 2684, 3560, 4330
BspPI GGATC 9 cut(s) 12, 1368, 1381, 1964, 2506, 3007, 3096, 3109, 4364
BspT107I GGYRCC 4 cut(s) 574, 3965, 4098, 4720
BspTI CTTAAG 1 cut(s) 1514
BspTNI GGTCTC 1 cut(s) 515
BsrBI CCGCTC 1 cut(s) 414
BsrDI GCAATG 5 cut(s) 1152, 1295, 2899, 3592, 4901
BsrFI RCCGGY 2 cut(s) 30, 1070
BssAI RCCGGY 2 cut(s) 30, 1070
BssNI GRCGYC 2 cut(s) 748, 3913
BssT1I CCWWGG 4 cut(s) 1864, 2992, 4341, 4979
Bst2UI CCWGG 4 cut(s) 1378, 2040, 2285, 4455
Bst6I CTCTTC 2 cut(s) 4380, 4491
BstACI GRCGYC 2 cut(s) 748, 3913
BstAFI CTTAAG 1 cut(s) 1514
BstAPI GCANNNNNTGC 3 cut(s) 1073, 2231, 3693
BstBAI YACGTR 3 cut(s) 127, 2802, 3380
BstDSI CCRYGG 1 cut(s) 4341
BstENI CCTNNNNNAGG 1 cut(s) 3841
BstFNI CGCG 1 cut(s) 3583
BstHHI GCGC 2 cut(s) 2833, 3585
BstMAI GTCTC 5 cut(s) 89, 515, 739, 3323, 3525
BstMCI CGRYCG 2 cut(s) 35, 626
BstNI CCWGG 4 cut(s) 1378, 2040, 2285, 4455
BstNSI RCATGY 8 cut(s) 1027, 1131, 2034, 2072, 2470, 4487, 4811, 4916
BstSCI CCNGG 8 cut(s) 506, 1376, 2038, 2283, 2300, 2738, 4453, 5028
BstSFI CTRYAG 4 cut(s) 2677, 3078, 3195, 3882
BstSLI GKGCMC 4 cut(s) 72, 201, 1397, 2807
BstUI CGCG 1 cut(s) 3583
BstV1I GCAGC 8 cut(s) 2209, 2218, 2221, 2579, 2996, 3064, 3181, 3493
BstV2I GAAGAC 3 cut(s) 549, 1833, 3956
BstX2I RGATCY 6 cut(s) 4, 1373, 2511, 3101, 4134, 4663
BstXI CCANNNNNNTGG 1 cut(s) 4949
BstYI RGATCY 6 cut(s) 4, 1373, 2511, 3101, 4134, 4663
BstZI CGGCCG 1 cut(s) 32
Bsu15I ATCGAT 1 cut(s) 104
BsuI GTATCC 3 cut(s) 3031, 4435, 4609
BsuTUI ATCGAT 1 cut(s) 104
BtgI CCRYGG 1 cut(s) 4341
BtgZI GCGATG 1 cut(s) 2548
BtrI CACGTC 1 cut(s) 2828
BtsI GCAGTG 2 cut(s) 590, 2686
BtsIMutI CAGTG 9 cut(s) 590, 837, 2059, 2097, 2686, 2805, 3785, 4338, 5030
BveI ACCTGC 3 cut(s) 2684, 3560, 4330
CaiI CAGNNNCTG 2 cut(s) 3606, 4928
CfoI GCGC 2 cut(s) 2833, 3585
Cfr10I RCCGGY 2 cut(s) 30, 1070
ClaI ATCGAT 1 cut(s) 104
CseI GACGC 1 cut(s) 2072
Csp6I GTAC 9 cut(s) 124, 999, 1491, 1875, 1888, 2249, 3603, 3633, 4099
CspAI ACCGGT 1 cut(s) 1070
CspCI CAANNNNNGTGG 2 cut(s) 2128, 2163
CviQI GTAC 9 cut(s) 124, 999, 1491, 1875, 1888, 2249, 3603, 3633, 4099
DraI TTTAAA 1 cut(s) 2179
EaeI YGGCCR 4 cut(s) 32, 1741, 2372, 4833
EagI CGGCCG 1 cut(s) 32
Eam1104I CTCTTC 2 cut(s) 4380, 4491
EarI CTCTTC 2 cut(s) 4380, 4491
EciI GGCGGA 2 cut(s) 2639, 2860
EclXI CGGCCG 1 cut(s) 32
Eco130I CCWWGG 4 cut(s) 1864, 2992, 4341, 4979
Eco147I AGGCCT 1 cut(s) 3811
Eco24I GRGCYC 1 cut(s) 72
Eco31I GGTCTC 1 cut(s) 515
Eco47I GGWCC 5 cut(s) 853, 1099, 1984, 2795, 3035
Eco52I CGGCCG 1 cut(s) 32
Eco57I CTGAAG 4 cut(s) 2127, 2274, 3958, 4128
Eco88I CYCGRG 5 cut(s) 71, 2268, 3518, 3889, 3992
EcoNI CCTNNNNNAGG 1 cut(s) 3841
EcoO109I RGGNCCY 3 cut(s) 68, 2795, 4814
EcoRI GAATTC 1 cut(s) 4595
EcoRII CCWGG 4 cut(s) 1376, 2038, 2283, 4453
EcoT14I CCWWGG 4 cut(s) 1864, 2992, 4341, 4979
EcoT22I ATGCAT 3 cut(s) 2191, 2468, 4554
EcoT38I GRGCYC 1 cut(s) 72
ErhI CCWWGG 4 cut(s) 1864, 2992, 4341, 4979
Esp3I CGTCTC 2 cut(s) 89, 739
FalI AAGNNNNNCTT 2 cut(s) 4254, 4286
FaqI GGGAC 4 cut(s) 132, 1306, 3515, 3900
FauI CCCGC 3 cut(s) 39, 58, 3503
FauNDI CATATG 1 cut(s) 4044
FbaI TGATCA 1 cut(s) 3678
FblI GTMKAC 1 cut(s) 3548
FriOI GRGCYC 1 cut(s) 72
FseI GGCCGGCC 1 cut(s) 34
FspBI CTAG 4 cut(s) 471, 1202, 3747, 4403
GlaI GCGC 2 cut(s) 2832, 3584
GsaI CCCAGC 5 cut(s) 530, 1803, 1963, 3109, 4452
GsuI CTGGAG 2 cut(s) 2427, 4314
HgaI GACGC 1 cut(s) 2072
HhaI GCGC 2 cut(s) 2833, 3585
Hin1I GRCGYC 2 cut(s) 748, 3913
Hin6I GCGC 2 cut(s) 2831, 3583
HinP1I GCGC 2 cut(s) 2831, 3583
HincII GTYRAC 3 cut(s) 2002, 2050, 3566
HindII GTYRAC 3 cut(s) 2002, 2050, 3566
HindIII AAGCTT 2 cut(s) 2390, 3207
HphI GGTGA 6 cut(s) 272, 467, 1723, 1880, 1948, 4652
Hpy99I CGWCG 1 cut(s) 3915
HpyCH4IV ACGT 8 cut(s) 126, 748, 2046, 2362, 2801, 2827, 3379, 3913
HpySE526I ACGT 8 cut(s) 126, 748, 2046, 2362, 2801, 2827, 3379, 3913
Hsp92I GRCGYC 2 cut(s) 748, 3913
HspAI GCGC 2 cut(s) 2831, 3583
Kpn2I TCCGGA 1 cut(s) 3955
KpnI GGTACC 1 cut(s) 4102
KroI GCCGGC 1 cut(s) 30
KroNI GCCGGC 1 cut(s) 32
Ksp22I TGATCA 1 cut(s) 3678
Lsp1109I GCAGC 8 cut(s) 2209, 2218, 2221, 2579, 2996, 3064, 3181, 3493
MaeI CTAG 4 cut(s) 471, 1202, 3747, 4403
MaeII ACGT 8 cut(s) 126, 748, 2046, 2362, 2801, 2827, 3379, 3913
MbiI CCGCTC 1 cut(s) 414
MfeI CAATTG 3 cut(s) 1451, 2016, 2727
MflI RGATCY 6 cut(s) 4, 1373, 2511, 3101, 4134, 4663
MhlI GDGCHC 8 cut(s) 72, 201, 697, 1078, 1397, 1450, 1479, 2807
MlsI TGGCCA 3 cut(s) 1743, 2374, 4835
MluNI TGGCCA 3 cut(s) 1743, 2374, 4835
MlyI GAGTC 6 cut(s) 3881, 3946, 4136, 4274, 4758, 4997
MmeI TCCRAC 5 cut(s) 1024, 1408, 2791, 3767, 4554
Mox20I TGGCCA 3 cut(s) 1743, 2374, 4835
Mph1103I ATGCAT 3 cut(s) 2191, 2468, 4554
MroI TCCGGA 1 cut(s) 3955
MroNI GCCGGC 1 cut(s) 30
MroXI GAANNNNTTC 2 cut(s) 902, 2645
MscI TGGCCA 3 cut(s) 1743, 2374, 4835
MslI CAYNNNNRTG 6 cut(s) 254, 572, 793, 1484, 1537, 2073
Msp20I TGGCCA 3 cut(s) 1743, 2374, 4835
MspA1I CMGCKG 2 cut(s) 530, 3484
MspCI CTTAAG 1 cut(s) 1514
MspR9I CCNGG 8 cut(s) 508, 1378, 2040, 2285, 2302, 2740, 4455, 5030
MunI CAATTG 3 cut(s) 1451, 2016, 2727
Mva1269I GAATGC 1 cut(s) 249
MvaI CCWGG 4 cut(s) 1378, 2040, 2285, 4455
MvnI CGCG 1 cut(s) 3583
NaeI GCCGGC 1 cut(s) 32
NciI CCSGG 4 cut(s) 508, 2302, 2740, 5030
NcoI CCATGG 1 cut(s) 4341
NdeI CATATG 1 cut(s) 4044
NgoMIV GCCGGC 1 cut(s) 30
NmeAIII GCCGAG 1 cut(s) 60
NmuCI GTSAC 7 cut(s) 329, 832, 1746, 1954, 2092, 3793, 4517
NsiI ATGCAT 3 cut(s) 2191, 2468, 4554
NspI RCATGY 8 cut(s) 1027, 1131, 2034, 2072, 2470, 4487, 4811, 4916
PaeI GCATGC 3 cut(s) 1131, 2072, 2470
PaeR7I CTCGAG 1 cut(s) 3518
PceI AGGCCT 1 cut(s) 3811
PcsI WCGNNNNNNNCGW 2 cut(s) 4249, 4749
PctI GAATGC 1 cut(s) 249
PdiI GCCGGC 1 cut(s) 32
PdmI GAANNNNTTC 2 cut(s) 902, 2645
PinAI ACCGGT 1 cut(s) 1070
PleI GAGTC 6 cut(s) 3881, 3946, 4135, 4274, 4757, 4996
PpsI GAGTC 6 cut(s) 3881, 3946, 4135, 4274, 4757, 4996
Ppu21I YACGTR 3 cut(s) 127, 2802, 3380
PpuMI RGGWCCY 1 cut(s) 2795
PshBI ATTAAT 2 cut(s) 3981, 4958
PsiI TTATAA 1 cut(s) 3822
Psp5II RGGWCCY 1 cut(s) 2795
Psp6I CCWGG 4 cut(s) 1376, 2038, 2283, 4453
PspFI CCCAGC 5 cut(s) 526, 1799, 1959, 3105, 4448
PspGI CCWGG 4 cut(s) 1376, 2038, 2283, 4453
PspOMI GGGCCC 1 cut(s) 68
PspPPI RGGWCCY 1 cut(s) 2795
PsrI GAACNNNNNNTAC 2 cut(s) 3625, 3657
PstI CTGCAG 3 cut(s) 2681, 3082, 3199
PstNI CAGNNNCTG 2 cut(s) 3606, 4928
PsuI RGATCY 6 cut(s) 4, 1373, 2511, 3101, 4134, 4663
PvuII CAGCTG 1 cut(s) 530
RigI GGCCGGCC 1 cut(s) 34
RsaI GTAC 9 cut(s) 125, 1000, 1492, 1876, 1889, 2250, 3604, 3634, 4100
RsaNI GTAC 9 cut(s) 124, 999, 1491, 1875, 1888, 2249, 3603, 3633, 4099
RseI CAYNNNNRTG 6 cut(s) 254, 572, 793, 1484, 1537, 2073
SchI GAGTC 6 cut(s) 3881, 3946, 4136, 4274, 4758, 4997
ScrFI CCNGG 8 cut(s) 508, 1378, 2040, 2285, 2302, 2740, 4455, 5030
SduI GDGCHC 8 cut(s) 72, 201, 697, 1078, 1397, 1450, 1479, 2807
SfcI CTRYAG 4 cut(s) 2677, 3078, 3195, 3882
Sfr274I CTCGAG 1 cut(s) 3518
SinI GGWCC 5 cut(s) 853, 1099, 1984, 2795, 3035
SlaI CTCGAG 1 cut(s) 3518
SmiMI CAYNNNNRTG 6 cut(s) 254, 572, 793, 1484, 1537, 2073
SmlI CTYRAG 6 cut(s) 742, 1514, 2708, 3518, 4121, 4390
SmoI CTYRAG 6 cut(s) 742, 1514, 2708, 3518, 4121, 4390
SpeI ACTAGT 1 cut(s) 3746
SphI GCATGC 3 cut(s) 1131, 2072, 2470
SseBI AGGCCT 1 cut(s) 3811
SspMI CTAG 4 cut(s) 471, 1202, 3747, 4403
StuI AGGCCT 1 cut(s) 3811
StyD4I CCNGG 8 cut(s) 506, 1376, 2038, 2283, 2300, 2738, 4453, 5028
StyI CCWWGG 4 cut(s) 1864, 2992, 4341, 4979
TaiI ACGT 8 cut(s) 129, 751, 2049, 2365, 2804, 2830, 3382, 3916
TaqII GACCGA 1 cut(s) 2037
TatI WGTACW 3 cut(s) 998, 1874, 2248
TauI GCSGC 3 cut(s) 46, 538, 541
TscAI CASTG 9 cut(s) 590, 837, 2059, 2097, 2686, 2812, 3792, 4338, 5030
TseFI GTSAC 7 cut(s) 329, 832, 1746, 1954, 2092, 3793, 4517
TseI GCWGC 8 cut(s) 2222, 2231, 2234, 2567, 2984, 3077, 3194, 3481
Tsp45I GTSAC 7 cut(s) 329, 832, 1746, 1954, 2092, 3793, 4517
TspGWI ACGGA 1 cut(s) 3114
TspRI CASTG 9 cut(s) 590, 837, 2059, 2097, 2686, 2812, 3792, 4338, 5030
Vha464I CTTAAG 1 cut(s) 1514
VneI GTGCAC 3 cut(s) 197, 1393, 2803
VpaK11BI GGWCC 5 cut(s) 853, 1099, 1984, 2795, 3035
VspI ATTAAT 2 cut(s) 3981, 4958
XagI CCTNNNNNAGG 1 cut(s) 3841
XapI RAATTY 4 cut(s) 1195, 2632, 3009, 4595
XceI RCATGY 8 cut(s) 1027, 1131, 2034, 2072, 2470, 4487, 4811, 4916
XcmI CCANNNNNNNNNTGG 1 cut(s) 1374
XhoI CTCGAG 1 cut(s) 3518
XmiI GTMKAC 1 cut(s) 3548
XmnI GAANNNNTTC 2 cut(s) 902, 2645
XspI CTAG 4 cut(s) 471, 1202, 3747, 4403
ZraI GACGTC 2 cut(s) 749, 3914
Zsp2I ATGCAT 3 cut(s) 2191, 2468, 4554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.