RchiOBHm_Chr5g0055621

WSTF, HB1, Itc1p, MBD9 motif 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0007748
Physical Location & Seq
Forward (+)
58930247 .. 58938413
8167 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33260

Sequence Viewer

Length: 5049 bp
ATGGATCCTCCGGTGGCGAAAAAGAGGGGCCGGCCGAGGAAGCGGCGGGCTGATGATCGGAAAGCGGGCCCCGAGGCGAAGAAGCAGGCATTGGAGACGAGATCGATTGCGTTGTTGGGACGGTACGTGTTGAAAGAGTTTGAGAGTAGGATTTATCTGGGGAAGGTTGCGTTTTATGATGCTGGATTGTATAGAGTGCACTATGAAGATGGGGATTTCGAGGACTTGGAGAGTGGTGAGATTCGGGGCATTCTTGTGGGTGAGAGTGATTTTGATGGGAGTTTGAGTGCTAAGAGGAAGAAATTGGATAAGCTGGTGGCGAAAGTGAGTGACGAAAAGAAGGTTAAGGTGGATGAGAATGTGGAAAAGGAGGTGGTGGTGGATAGGGTTGAATCCTCGGAATTGAGTGAGTGGAGCGGTAGAGTAACTATTGACAATGATGAGATTCGAGAGGATGGTGATGCTGATTTGTCTAGTGATTCGAGTGAGTGTGCAGAGGATATGGATGCCGGGGTTGATGTTGAGACCCCGGCTGTGCTGCCGCCGGAGTTGCCACCGTCTTCTGGAACCATAGGCGTGCCGGAGCAGTGTGTTTCGCATCTTTTCTCGGTGTATGGTTTCATGCGGTCGTTTAGCATCTGCCTGTTCTTGAACCCATTTACTTTGGATGATTTTGTGGGTTCGCTTAATTGTCGTGCTCCAAACACATTGTTTGATGCAATTCATGTTGCTTTGTTGCGTGCCTTGAGACGTCATCTTGAAACAATCTCATCCGAAGGCTCAGAGGTAGCACAAAAATGTTTGAGGTGCATAGATTGGAGCTTGCTCGATACAGTGACTTGGCCTGTTTACTTGGTCCAGTATTTAACAATTATGGGATATGTAAAAGGACAAGAGTGGAAAGGTTTCTATGACGAGGTTTTGGACAGAGAGTATTATTTATTATCAGTTGATAGGAAGTTAAGCATTTTGCAAATCATTTGTGATGATGTCTTAGACAGTATAGATATAAGAGCTGAATTAGACATGCGTGAAGAATCAGAAGTTGGAATAGATTATGATGCCGAAGCAACCAGTGCTCTTGTAAATGGGCCAAGAAGGGTCCATCCGAGATATTCCAAAACTTCTGCATGCAAGGACAGAGAAGCAATGGAAATTGCTACTGAATCCCATGATATAAAATTATCTGGTAACTTAAATTCACTAGGTTCCAAAGTTGCTAAAGGGGAGTTTGATGCTACTGATGTTGATTCAGATTGTAACTCTGATGAATGTCGGCTTTGTGGTATGGATGGGACATTGCTTTGCTGTGATGGATGCCCATCCGCCTACCATACGAGGTGTATAGGTGTGATGAAACTGTCGATACCAGAAGGATCCTGGTATTGTCCTGAGTGCACTATCAACAAAATTGGGCCAACTATCACAGTTGGAACATCAGTTAAAGGAGCACAATTGTTTGGGATAGATGCCTACGAGCACATCTTCATGGGTACTTGCAACCACTTACTTGTGCTTAAGGAAACTATTAACACACAACCATGTCTTAGATATTACAATCAGCATGACATTCCAAAAGTCCTGAAAGTTCTTTGTTCATCTGGGCAAAATACATCTTTTTATCTGGGCTTGTGCAAGGCAATTTTAGAATACTGGAATATCCCAGACAGCATCTTATCTCTTTCAGAGCTGGCTGAATCAATGACTAATTTGGCGAAGATAAAGGAAGATGCGAACTTTTGTGGCCAGTCACTTCCTTTGTCTGGCAAGGATTGTGATAAAGTTATGGATATGGTTGAGGCTGGGAACTGTGAAACTTATGTAAATGAAGACCATTTAGGTAAATCAGTTTCATCTTACAATTGCCATGGTGATAGTACACCACAGGAGTACTCACATAGGAATATGGAGATTGATGAAAGGAATAAAATGGAATCTGCTGTTTCGACTGGTTCTGTCACCCAGCAAGCTGATCCATCTTACTTGGTCCACAGAAGCTCAGTTGACAGGCCAACTGCAATTGACCGAAGCACATGCACACCAGGGAACGTCAACAGTGGTAACAAAGTGCATGCAAATGGTATGCGTCTTTCAGTGACTTCACAAAATGAAGAAGGCAATCATATAGGAAAGGTTTACTCCACTTCAGTTGATGATTGCAAATACAAGGGTTCTTTCTTTAAACCCCATGCATACATAAATTACTATGTGCATGGGGATTTTGCTGCATCTGCTGCTGCCAAACTGGCTGTACTTTCATCAGAAGAAGCTCGGGGTTCAGATGTCCAGGCTTCAGGCAATCCCCGGAAAGTTGCATCATCAAACAATTTATGGCAAGCAAAAGCCTTCTCACTAACAGCCTCACGTTTCTTCTGGCCAAGTTTTGAAAAGAAGCTTGTGGAGGTTCCAAGAGAGAGGTGTGGTTGGTGTCTTTCTTGTAAGGCTCCAGTTGCAAGCAAGAGAGGATGCATGCTAAATCATGCTTGTTTGAGTGCCACTAAAGGTGCTATGAAGATCCTTGCCAGCCTTCGTCCTATAAAGAGTGTGGAGGGGAATCTTGTAAGCATCGCAACATACATCTTATACATGGAGGAGAGTTTGCATGGTCTAATAACTGGCCCCTTTCTCAATGAAAATTTTAGGAAACAATTCCGCCAACAAGTTTATCAAGCATCAACCTGCAGTGCAATAAAAGTCCTATTGCTCAAACTTGAGGAAAACATCCGAACAATTGCTCTTTCTGGGGAGTGGATTAAACTGGTAGATGATTTGCTGGTTGAATCTTCTGTGATTCAAGGTCCTACATGCACTGTTGGAACAACACAAAGACGTGCACCATACAGCAGGCGGAGCAGGAAACAGAGTGCAATTCAAGAAGTTATAGATGATGACTGCAATGACAAGAGTTTTGTATGGTGGCAAGGAGGGAAGCTGTCAAAAATCATATTCCAGAGAGCAATCTTGGCTCGCTCATTAGTTAAAAAAGCAGCTCGCCAAGGTGGATCGAGGAAAATTTTTGGTGTATCTTACGCTGATGGTCCTGATATTCCTAAAAGAAGCAGACAATCTGTATGGAGGGCTGCAGTTGAACTGAGCAAGAACGGATCCCAGCTTGCAGTTCAGGTTAGATACTTGGACTATCATTTGAGGTGGAGTGATCTTGTTCGTCCTGAGCAGAACCTCCTTGATGGGAAAGCTGCAGAGGCAGAAGCTTCTGCTTTTAGAAATGCAAGTATATGTGATAAAAAAATGTTAAAGAACAACATCGTCTATGGGGTTGCATTTGGTAGTCAAAAGCATCTGCCTAACCGTGTAATGAAAAATATCATTGAGACAGAGCAGAATCAGGATGGAACTAACAAGTTCTGGTTTCTTGAATCACGTATTCCTTTATATTTGATTAAGGAATATGAAGAAAGTGTAGGTAAAGTGTCTATTCCATCAGCTCAGGAGCCTAATCTATTGCACAAGTTAAAGAGAAGGCAGCGGAAAGCAACCTGCAGGGACATATTTTTTTATCTCGAGTGTAAAAGAGACAATCTGGACTTGTCTACCTGCTCTATGTGTCAACTGGATATTTTATTGCGCAATGCAGTCAAGTGCAGTGCCTGTCAAGGTTATTGCCATGAGGGCTGTACCATAAGTTCAACAGTTTCTACAAATGAGGAAGTTGAGTTCTTGATCACTTGCAAGCAGTGCTACCATGCGAAAGCTCTTACCCAGAAGCAAAAGTTTAAGGAGTTTCCAACTAGTCCTTTGCCCTTGCAAATGAAAGAATATCATACTCCACTGACAGTCACAACTGGTGGAAGGCCTAAAAATTATAACCAATCATTAACCTCCATCAGGGTTCAGGAGCCTCGTTCTGAGATTAAACAAGCTACTAGAGACTCGAGTTTGGCAACAAAAAAACGACGTCCCATTTGTTCTTGGGGTGTTATATGGAAGAAGAAGACTCCGGAAACTGGCACTGACTTCAGGATTAATAACATACTCCTCGGGGGTCGTTCGGATGTGCATGGGTTGAAACCTGTTTGCCACTTGTGCCATATGCCATATCTCTCCGATCTGACATATATACGCTGTGAATCTTGCAAAAATTGGTACCATGCTGAAGCTGTCGAACTTGAGGAGTCAAAGATCTGTGAAGTGGCAGGCTTCAAGTGTTGCAAGTGTCGCAGGATAAAATCGCCTCTTTGTCCTTATACTGATCTTAAAGACAAGACCTTGCAAGAGAGCAAGAAGATTCGCAATAAGCGTTCAAAGCAAGAAAACCTTGGAGAGGACTCGGATTCTGCAACCAATTTTGATTCAGAGGTGTTTGAACCTGCTACTCCAGTGTTTCCCATGGAGGAAGTCTCCATTCAGGATGATGATCCTCTTCTTTTTGCTCTCTCAAGAGTGGAGCTAGTTCCAGAACACAATTCAGAAGTTAATGCTGAATGGGATACTGCTGGGCCAGGGCCACGAAAATTACCTGTGAGAAGGCATGTTAAGCGTGAAGAGGACTTGGATATATATTGTGACAGTAATAAATCTCATGCTGAAAAAACTATGCATGATGAAACAAATTACCTGTCGGAACCGATGGAGATTGCGGCATTCCCTCATGTGGAATGGGATACTTCCATGAACGGCATCAATGGTGAGATAATGGAACAGTATGAAGATCTGAACTACGATATGATGGAACCGCAAACTGTCTTTACTATCAATGAGTTGCTGGCACCTGATGACGGCGACCTTTTTGATGGAGTTGAAACATTTGCGGATTTACCAGAAAATCTGGAAAATCCATATACAACTTCACAGCATGTAGGCCCTGAGCAATATGATATGGCCACTTTCACAGACGAATCAAAGTCTGCAATTCCTGAAACATCTGCTGTTAATATGATGCAATGTCAGATATGTTTACTTGCAGAACCAGCTCCTGATCTTTCTTGCCAGAACTGTGGGCTATTAATTCATAACCGTTGTTCCCCTTGGATTGAGTCATCATCTCAGAATAATAGCTGGAAGTGTGGACAGTGCCGGGAGTGGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000122 GO:0000228 GO:0000785 GO:0000790 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0006139 GO:0006325 GO:0006338 GO:0006351 GO:0006355 GO:0006357 GO:0006725 GO:0006807 GO:0006996 GO:0007154 GO:0007165 GO:0007275 GO:0007399 GO:0007417 GO:0007420 GO:0008134 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009526 GO:0009527 GO:0009536 GO:0009707 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009941 GO:0009987 GO:0010019 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0016020 GO:0016043 GO:0016070 GO:0016589 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019867 GO:0023052 GO:0031010 GO:0031090 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031967 GO:0031968 GO:0031969 GO:0031974 GO:0031975 GO:0031981 GO:0032501 GO:0032502 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0035064 GO:0042170 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043565 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044454 GO:0044464 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0046483 GO:0048513 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051173 GO:0051252 GO:0051253 GO:0051254 GO:0051276 GO:0051716 GO:0060255 GO:0060322 GO:0065007 GO:0070013 GO:0070603 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:0098588 GO:0098805 GO:0140030 GO:0140034 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902494 GO:1902679 GO:1902680 GO:1903506 GO:1903507 GO:1903508 GO:1904949 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDT PHD WHIM1

Protein Analysis

1682

Amino Acids

188.82

Weight (kDa)

5.74

Isoelectric Point (pI)

45.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTM_DIR17_Tudor PF21743 40 - 85 6.2e-15 PTM/DIR17-like, Tudor domain
DDT PF02791 196 - 252 5e-18 DDT domain
WHIM1 PF15612 298 - 342 8.7e-10 WSTF, HB1, Itc1p, MBD9 motif 1
PHD PF00628 425 - 466 5.7e-08 PHD-finger
Chromo_PTM PF24294 1071 - 1143 4.3e-33 Membrane-bound transcription factor PTM, chromo domain-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3825
AatII GACGTC 2 cut(s) 754, 3919
Acc16I TGCGCA 1 cut(s) 3587
Acc36I ACCTGC 4 cut(s) 2687, 3506, 3563, 4336
Acc65I GGTACC 1 cut(s) 4104
AccB1I GGYRCC 2 cut(s) 4104, 4726
AccBSI CCGCTC 1 cut(s) 417
AccI GTMKAC 1 cut(s) 3551
AccIII TCCGGA 1 cut(s) 3958
AclWI GGATC 9 cut(s) 12, 1371, 1384, 1967, 2509, 3010, 3099, 3112, 4370
AcoI YGGCCR 4 cut(s) 32, 1744, 2375, 4839
AcsI RAATTY 3 cut(s) 1198, 2635, 3012
AcuI CTGAAG 4 cut(s) 2130, 2277, 3961, 4134
AcyI GRCGYC 2 cut(s) 751, 3916
AfaI GTAC 7 cut(s) 125, 1495, 1879, 1892, 2253, 3637, 4106
AfiI CCNNNNNNNGG 7 cut(s) 563, 1763, 3846, 3965, 4282, 4612, 4736
AflII CTTAAG 1 cut(s) 1517
AflIII ACRYGT 1 cut(s) 126
AhlI ACTAGT 1 cut(s) 3749
AjiI CACGTC 1 cut(s) 2831
AjnI CCWGG 4 cut(s) 1379, 2041, 2286, 4459
AjuI GAANNNNNNNTTGG 4 cut(s) 74, 106, 1029, 1061
AloI GAACNNNNNNTCC 2 cut(s) 3659, 3691
Alw21I GWGCWC 7 cut(s) 201, 700, 1081, 1400, 1453, 1482, 2836
Alw26I GTCTC 7 cut(s) 89, 518, 742, 3326, 3528, 3882, 4363
Alw44I GTGCAC 3 cut(s) 197, 1396, 2832
AlwI GGATC 9 cut(s) 12, 1371, 1384, 1967, 2509, 3010, 3099, 3112, 4370
AlwNI CAGNNNCTG 2 cut(s) 3609, 4934
Ama87I CYCGRG 5 cut(s) 71, 2271, 3521, 3892, 3998
Aor13HI TCCGGA 1 cut(s) 3958
ApaI GGGCCC 1 cut(s) 71
ApaLI GTGCAC 3 cut(s) 197, 1396, 2832
ApeKI GCWGC 8 cut(s) 538, 2225, 2234, 2237, 2987, 3080, 3197, 3484
ApoI RAATTY 3 cut(s) 1198, 2635, 3012
ArsI GACNNNNNNTTYG 6 cut(s) 2891, 2923, 3906, 3938, 4562, 4594
AseI ATTAAT 2 cut(s) 3984, 4964
Asp700I GAANNNNTTC 2 cut(s) 905, 2648
Asp718I GGTACC 1 cut(s) 4104
AspLEI GCGC 1 cut(s) 3588
AsuC2I CCSGG 4 cut(s) 511, 530, 2305, 5036
AsuHPI GGTGA 6 cut(s) 248, 272, 470, 1883, 1951, 4658
AvaI CYCGRG 5 cut(s) 71, 2271, 3521, 3892, 3998
AvaII GGWCC 5 cut(s) 856, 1102, 1987, 2798, 3038
BaeGI GKGCMC 4 cut(s) 71, 201, 1400, 2836
BalI TGGCCA 3 cut(s) 1746, 2377, 4841
BamHI GGATCC 3 cut(s) 4, 1376, 3104
BanI GGYRCC 2 cut(s) 4104, 4726
BanII GRGCYC 1 cut(s) 71
BbsI GAAGAC 3 cut(s) 552, 1836, 3959
Bbv12I GWGCWC 7 cut(s) 201, 700, 1081, 1400, 1453, 1482, 2836
BbvI GCAGC 8 cut(s) 525, 2212, 2221, 2224, 2999, 3067, 3184, 3496
BceAI ACGGC 2 cut(s) 4651, 4753
BcgI CGANNNNNNTGC 2 cut(s) 2016, 2050
BciT130I CCWGG 4 cut(s) 1381, 2043, 2288, 4461
BciVI GTATCC 2 cut(s) 4441, 4615
BclI TGATCA 1 cut(s) 3681
BcnI CCSGG 4 cut(s) 511, 530, 2305, 5036
BcoDI GTCTC 7 cut(s) 89, 518, 742, 3326, 3528, 3882, 4363
BcuI ACTAGT 1 cut(s) 3749
BfaI CTAG 5 cut(s) 474, 1205, 3750, 3885, 4409
BfmI CTRYAG 4 cut(s) 2680, 3081, 3198, 3499
BfrI CTTAAG 1 cut(s) 1517
BfuAI ACCTGC 4 cut(s) 2687, 3506, 3563, 4336
BfuI GTATCC 2 cut(s) 4441, 4615
BglI GCCNNNNNGGC 2 cut(s) 2246, 3630
BglII AGATCT 2 cut(s) 4140, 4669
BmcAI AGTACT 1 cut(s) 1892
Bme1390I CCNGG 8 cut(s) 511, 530, 1381, 2043, 2288, 2305, 4461, 5036
Bme18I GGWCC 5 cut(s) 856, 1102, 1987, 2798, 3038
BmeT110I CYCGRG 5 cut(s) 71, 2271, 3521, 3892, 3998
BmgBI CACGTC 1 cut(s) 2831
BmrFI CCNGG 8 cut(s) 511, 530, 1381, 2043, 2288, 2305, 4461, 5036
BpiI GAAGAC 3 cut(s) 552, 1836, 3959
BplI GAGNNNNNCTC 2 cut(s) 4343, 4375
BpmI CTGGAG 2 cut(s) 2430, 4320
Bpu10I CCTNAGC 3 cut(s) 3171, 3447, 4824
BpuEI CTTGAG 4 cut(s) 766, 2732, 4148, 4381
BpuMI CCSGG 4 cut(s) 511, 530, 2305, 5036
Bsa29I ATCGAT 1 cut(s) 104
BsaAI YACGTR 2 cut(s) 127, 3383
BsaBI GATNNNNATC 2 cut(s) 1321, 4374
BsaHI GRCGYC 2 cut(s) 751, 3916
BsaI GGTCTC 1 cut(s) 518
BsaWI WCCGGW 2 cut(s) 10, 3958
BsaXI ACNNNNNCTCC 3 cut(s) 271, 301, 5030
Bsc4I CCNNNNNNNGG 7 cut(s) 563, 1763, 3846, 3965, 4282, 4612, 4736
Bse118I RCCGGY 1 cut(s) 30
Bse3DI GCAATG 5 cut(s) 1155, 1298, 2902, 3595, 4907
Bse8I GATNNNNATC 2 cut(s) 1321, 4374
BseAI TCCGGA 1 cut(s) 3958
BseBI CCWGG 4 cut(s) 1381, 2043, 2288, 4461
BseCI ATCGAT 1 cut(s) 104
BseJI GATNNNNATC 2 cut(s) 1321, 4374
BseLI CCNNNNNNNGG 7 cut(s) 563, 1763, 3846, 3965, 4282, 4612, 4736
BseMI GCAATG 5 cut(s) 1155, 1298, 2902, 3595, 4907
BseMII CTCAG 9 cut(s) 795, 1383, 2013, 3083, 3162, 3461, 3858, 4815, 5018
BseRI GAGGAG 3 cut(s) 2606, 3986, 4145
BseSI GKGCMC 4 cut(s) 71, 201, 1400, 2836
BseX3I CGGCCG 1 cut(s) 32
BseXI GCAGC 8 cut(s) 525, 2212, 2221, 2224, 2999, 3067, 3184, 3496
BseYI CCCAGC 4 cut(s) 1802, 1962, 3108, 4454
BsgI GTGCAG 2 cut(s) 513, 3622
Bsh1285I CGRYCG 2 cut(s) 35, 629
BshNI GGYRCC 2 cut(s) 4104, 4726
BshVI ATCGAT 1 cut(s) 104
BsiEI CGRYCG 2 cut(s) 35, 629
BsiHKAI GWGCWC 7 cut(s) 201, 700, 1081, 1400, 1453, 1482, 2836
BsiHKCI CYCGRG 5 cut(s) 71, 2271, 3521, 3892, 3998
BsiSI CCGG 9 cut(s) 11, 31, 510, 530, 545, 581, 2305, 3959, 5035
BslFI GGGAC 4 cut(s) 132, 1309, 3518, 3903
BslI CCNNNNNNNGG 7 cut(s) 563, 1763, 3846, 3965, 4282, 4612, 4736
BsmAI GTCTC 7 cut(s) 89, 518, 742, 3326, 3528, 3882, 4363
BsmBI CGTCTC 2 cut(s) 89, 742
BsmFI GGGAC 4 cut(s) 132, 1309, 3518, 3903
BsmI GAATGC 2 cut(s) 249, 4601
Bso31I GGTCTC 1 cut(s) 518
BsoBI CYCGRG 5 cut(s) 71, 2271, 3521, 3892, 3998
Bsp120I GGGCCC 1 cut(s) 67
Bsp1286I GDGCHC 8 cut(s) 71, 201, 700, 1081, 1400, 1453, 1482, 2836
Bsp13I TCCGGA 1 cut(s) 3958
Bsp19I CCATGG 2 cut(s) 1867, 4347
BspCNI CTCAG 9 cut(s) 794, 1384, 2012, 3084, 3163, 3460, 3859, 4816, 5017
BspDI ATCGAT 1 cut(s) 104
BspEI TCCGGA 1 cut(s) 3958
BspMAI CTGCAG 4 cut(s) 2684, 3085, 3202, 3503
BspMI ACCTGC 4 cut(s) 2687, 3506, 3563, 4336
BspPI GGATC 9 cut(s) 12, 1371, 1384, 1967, 2509, 3010, 3099, 3112, 4370
BspT107I GGYRCC 2 cut(s) 4104, 4726
BspTI CTTAAG 1 cut(s) 1517
BspTNI GGTCTC 1 cut(s) 518
BsrBI CCGCTC 1 cut(s) 417
BsrDI GCAATG 5 cut(s) 1155, 1298, 2902, 3595, 4907
BsrFI RCCGGY 1 cut(s) 30
BssAI RCCGGY 1 cut(s) 30
BssNI GRCGYC 2 cut(s) 751, 3916
BssT1I CCWWGG 5 cut(s) 1867, 2995, 4276, 4347, 4985
Bst2UI CCWGG 4 cut(s) 1381, 2043, 2288, 4461
Bst6I CTCTTC 2 cut(s) 4386, 4497
BstACI GRCGYC 2 cut(s) 751, 3916
BstAFI CTTAAG 1 cut(s) 1517
BstAPI GCANNNNNTGC 3 cut(s) 1076, 2234, 3696
BstBAI YACGTR 2 cut(s) 127, 3383
BstDSI CCRYGG 2 cut(s) 1867, 4347
BstENI CCTNNNNNAGG 2 cut(s) 3844, 4280
BstHHI GCGC 1 cut(s) 3588
BstMAI GTCTC 7 cut(s) 89, 518, 742, 3326, 3528, 3882, 4363
BstMCI CGRYCG 2 cut(s) 35, 629
BstNI CCWGG 4 cut(s) 1381, 2043, 2288, 4461
BstNSI RCATGY 8 cut(s) 1030, 1134, 2037, 2075, 2473, 2808, 4493, 4817
BstSCI CCNGG 8 cut(s) 509, 528, 1379, 2041, 2286, 2303, 4459, 5034
BstSFI CTRYAG 4 cut(s) 2680, 3081, 3198, 3499
BstSLI GKGCMC 4 cut(s) 71, 201, 1400, 2836
BstV1I GCAGC 8 cut(s) 525, 2212, 2221, 2224, 2999, 3067, 3184, 3496
BstV2I GAAGAC 3 cut(s) 552, 1836, 3959
BstX2I RGATCY 6 cut(s) 4, 1376, 2514, 3104, 4140, 4669
BstXI CCANNNNNNTGG 1 cut(s) 4955
BstYI RGATCY 6 cut(s) 4, 1376, 2514, 3104, 4140, 4669
BstZI CGGCCG 1 cut(s) 32
Bsu15I ATCGAT 1 cut(s) 104
BsuI GTATCC 2 cut(s) 4441, 4615
BsuTUI ATCGAT 1 cut(s) 104
BtgI CCRYGG 2 cut(s) 1867, 4347
BtgZI GCGATG 1 cut(s) 2551
BtrI CACGTC 1 cut(s) 2831
BtsI GCAGTG 4 cut(s) 593, 2689, 3610, 3701
BveI ACCTGC 4 cut(s) 2687, 3506, 3563, 4336
CaiI CAGNNNCTG 2 cut(s) 3609, 4934
CfoI GCGC 1 cut(s) 3588
Cfr10I RCCGGY 1 cut(s) 30
ClaI ATCGAT 1 cut(s) 104
CseI GACGC 1 cut(s) 2075
Csp6I GTAC 7 cut(s) 124, 1494, 1878, 1891, 2252, 3636, 4105
CspCI CAANNNNNGTGG 2 cut(s) 2131, 2166
CviQI GTAC 7 cut(s) 124, 1494, 1878, 1891, 2252, 3636, 4105
DraI TTTAAA 1 cut(s) 2182
EaeI YGGCCR 4 cut(s) 32, 1744, 2375, 4839
EagI CGGCCG 1 cut(s) 32
Eam1104I CTCTTC 2 cut(s) 4386, 4497
EarI CTCTTC 2 cut(s) 4386, 4497
EciI GGCGGA 3 cut(s) 1315, 2642, 2863
EclXI CGGCCG 1 cut(s) 32
Eco130I CCWWGG 5 cut(s) 1867, 2995, 4276, 4347, 4985
Eco147I AGGCCT 1 cut(s) 3814
Eco24I GRGCYC 1 cut(s) 71
Eco31I GGTCTC 1 cut(s) 518
Eco47I GGWCC 5 cut(s) 856, 1102, 1987, 2798, 3038
Eco52I CGGCCG 1 cut(s) 32
Eco57I CTGAAG 4 cut(s) 2130, 2277, 3961, 4134
Eco88I CYCGRG 5 cut(s) 71, 2271, 3521, 3892, 3998
EcoNI CCTNNNNNAGG 2 cut(s) 3844, 4280
EcoO109I RGGNCCY 3 cut(s) 68, 2798, 4820
EcoRII CCWGG 4 cut(s) 1379, 2041, 2286, 4459
EcoT14I CCWWGG 5 cut(s) 1867, 2995, 4276, 4347, 4985
EcoT22I ATGCAT 3 cut(s) 2194, 2471, 4560
EcoT38I GRGCYC 1 cut(s) 71
ErhI CCWWGG 5 cut(s) 1867, 2995, 4276, 4347, 4985
Esp3I CGTCTC 2 cut(s) 89, 742
FalI AAGNNNNNCTT 2 cut(s) 4260, 4292
FaqI GGGAC 4 cut(s) 132, 1309, 3518, 3903
FauI CCCGC 2 cut(s) 39, 58
FauNDI CATATG 1 cut(s) 4050
FbaI TGATCA 1 cut(s) 3681
FblI GTMKAC 1 cut(s) 3551
FriOI GRGCYC 1 cut(s) 71
FseI GGCCGGCC 1 cut(s) 34
FspBI CTAG 5 cut(s) 474, 1205, 3750, 3885, 4409
FspI TGCGCA 1 cut(s) 3587
GlaI GCGC 1 cut(s) 3587
GsaI CCCAGC 4 cut(s) 1806, 1966, 3112, 4458
GsuI CTGGAG 2 cut(s) 2430, 4320
HapII CCGG 9 cut(s) 11, 31, 510, 530, 545, 581, 2305, 3959, 5035
HgaI GACGC 1 cut(s) 2075
HhaI GCGC 1 cut(s) 3588
Hin1I GRCGYC 2 cut(s) 751, 3916
Hin6I GCGC 1 cut(s) 3586
HinP1I GCGC 1 cut(s) 3586
HincII GTYRAC 3 cut(s) 2005, 2053, 3569
HindII GTYRAC 3 cut(s) 2005, 2053, 3569
HindIII AAGCTT 2 cut(s) 2393, 3210
HpaII CCGG 9 cut(s) 11, 31, 510, 530, 545, 581, 2305, 3959, 5035
HphI GGTGA 6 cut(s) 248, 272, 470, 1883, 1951, 4658
Hpy99I CGWCG 1 cut(s) 3918
HpyCH4IV ACGT 7 cut(s) 126, 751, 2049, 2365, 2830, 3382, 3916
HpySE526I ACGT 7 cut(s) 126, 751, 2049, 2365, 2830, 3382, 3916
Hsp92I GRCGYC 2 cut(s) 751, 3916
HspAI GCGC 1 cut(s) 3586
Kpn2I TCCGGA 1 cut(s) 3958
KpnI GGTACC 1 cut(s) 4108
KroI GCCGGC 1 cut(s) 30
KroNI GCCGGC 1 cut(s) 32
Ksp22I TGATCA 1 cut(s) 3681
Lsp1109I GCAGC 8 cut(s) 525, 2212, 2221, 2224, 2999, 3067, 3184, 3496
MaeI CTAG 5 cut(s) 474, 1205, 3750, 3885, 4409
MaeII ACGT 7 cut(s) 126, 751, 2049, 2365, 2830, 3382, 3916
MbiI CCGCTC 1 cut(s) 417
MfeI CAATTG 4 cut(s) 1454, 1861, 2019, 2730
MflI RGATCY 6 cut(s) 4, 1376, 2514, 3104, 4140, 4669
MhlI GDGCHC 8 cut(s) 71, 201, 700, 1081, 1400, 1453, 1482, 2836
MlsI TGGCCA 3 cut(s) 1746, 2377, 4841
MluNI TGGCCA 3 cut(s) 1746, 2377, 4841
MlyI GAGTC 5 cut(s) 3884, 3949, 4142, 4280, 5003
MmeI TCCRAC 5 cut(s) 1027, 1411, 2794, 3770, 4560
Mox20I TGGCCA 3 cut(s) 1746, 2377, 4841
Mph1103I ATGCAT 3 cut(s) 2194, 2471, 4560
MroI TCCGGA 1 cut(s) 3958
MroNI GCCGGC 1 cut(s) 30
MroXI GAANNNNTTC 2 cut(s) 905, 2648
MscI TGGCCA 3 cut(s) 1746, 2377, 4841
MslI CAYNNNNRTG 6 cut(s) 254, 575, 796, 1487, 1540, 2076
Msp20I TGGCCA 3 cut(s) 1746, 2377, 4841
MspA1I CMGCKG 1 cut(s) 3487
MspCI CTTAAG 1 cut(s) 1517
MspI CCGG 9 cut(s) 11, 31, 510, 530, 545, 581, 2305, 3959, 5035
MspR9I CCNGG 8 cut(s) 511, 530, 1381, 2043, 2288, 2305, 4461, 5036
MunI CAATTG 4 cut(s) 1454, 1861, 2019, 2730
Mva1269I GAATGC 2 cut(s) 249, 4601
MvaI CCWGG 4 cut(s) 1381, 2043, 2288, 4461
NaeI GCCGGC 1 cut(s) 32
NciI CCSGG 4 cut(s) 511, 530, 2305, 5036
NcoI CCATGG 2 cut(s) 1867, 4347
NdeI CATATG 1 cut(s) 4050
NgoMIV GCCGGC 1 cut(s) 30
NmeAIII GCCGAG 1 cut(s) 60
NmuCI GTSAC 7 cut(s) 329, 835, 1749, 1957, 2095, 3796, 4523
NsbI TGCGCA 1 cut(s) 3587
NsiI ATGCAT 3 cut(s) 2194, 2471, 4560
NspI RCATGY 8 cut(s) 1030, 1134, 2037, 2075, 2473, 2808, 4493, 4817
PaeI GCATGC 3 cut(s) 1134, 2075, 2473
PaeR7I CTCGAG 2 cut(s) 3521, 3892
PceI AGGCCT 1 cut(s) 3814
PcsI WCGNNNNNNNCGW 1 cut(s) 4255
PctI GAATGC 2 cut(s) 249, 4601
PdiI GCCGGC 1 cut(s) 32
PdmI GAANNNNTTC 2 cut(s) 905, 2648
PleI GAGTC 5 cut(s) 3884, 3949, 4141, 4280, 5002
PpsI GAGTC 5 cut(s) 3884, 3949, 4141, 4280, 5002
Ppu21I YACGTR 2 cut(s) 127, 3383
PpuMI RGGWCCY 1 cut(s) 2798
PshBI ATTAAT 2 cut(s) 3984, 4964
PsiI TTATAA 1 cut(s) 3825
Psp5II RGGWCCY 1 cut(s) 2798
Psp6I CCWGG 4 cut(s) 1379, 2041, 2286, 4459
PspFI CCCAGC 4 cut(s) 1802, 1962, 3108, 4454
PspGI CCWGG 4 cut(s) 1379, 2041, 2286, 4459
PspOMI GGGCCC 1 cut(s) 67
PspPPI RGGWCCY 1 cut(s) 2798
PspXI VCTCGAGB 1 cut(s) 3892
PsrI GAACNNNNNNTAC 2 cut(s) 3628, 3660
PstI CTGCAG 4 cut(s) 2684, 3085, 3202, 3503
PstNI CAGNNNCTG 2 cut(s) 3609, 4934
PsuI RGATCY 6 cut(s) 4, 1376, 2514, 3104, 4140, 4669
RigI GGCCGGCC 1 cut(s) 34
RsaI GTAC 7 cut(s) 125, 1495, 1879, 1892, 2253, 3637, 4106
RsaNI GTAC 7 cut(s) 124, 1494, 1878, 1891, 2252, 3636, 4105
RseI CAYNNNNRTG 6 cut(s) 254, 575, 796, 1487, 1540, 2076
SbfI CCTGCAGG 1 cut(s) 3503
ScaI AGTACT 1 cut(s) 1892
SchI GAGTC 5 cut(s) 3884, 3949, 4142, 4280, 5003
ScrFI CCNGG 8 cut(s) 511, 530, 1381, 2043, 2288, 2305, 4461, 5036
SdaI CCTGCAGG 1 cut(s) 3503
SduI GDGCHC 8 cut(s) 71, 201, 700, 1081, 1400, 1453, 1482, 2836
SfcI CTRYAG 4 cut(s) 2680, 3081, 3198, 3499
Sfr274I CTCGAG 2 cut(s) 3521, 3892
SinI GGWCC 5 cut(s) 856, 1102, 1987, 2798, 3038
SlaI CTCGAG 2 cut(s) 3521, 3892
SmiMI CAYNNNNRTG 6 cut(s) 254, 575, 796, 1487, 1540, 2076
SmlI CTYRAG 7 cut(s) 745, 1517, 2711, 3521, 3892, 4127, 4396
SmoI CTYRAG 7 cut(s) 745, 1517, 2711, 3521, 3892, 4127, 4396
SpeI ACTAGT 1 cut(s) 3749
SphI GCATGC 3 cut(s) 1134, 2075, 2473
Sse8387I CCTGCAGG 1 cut(s) 3503
SseBI AGGCCT 1 cut(s) 3814
SspMI CTAG 5 cut(s) 474, 1205, 3750, 3885, 4409
StuI AGGCCT 1 cut(s) 3814
StyD4I CCNGG 8 cut(s) 509, 528, 1379, 2041, 2286, 2303, 4459, 5034
StyI CCWWGG 5 cut(s) 1867, 2995, 4276, 4347, 4985
TaiI ACGT 7 cut(s) 129, 754, 2052, 2368, 2833, 3385, 3919
TaqII GACCGA 1 cut(s) 2040
TatI WGTACW 3 cut(s) 1877, 1890, 2251
TauI GCSGC 3 cut(s) 46, 544, 4601
TseFI GTSAC 7 cut(s) 329, 835, 1749, 1957, 2095, 3796, 4523
TseI GCWGC 8 cut(s) 538, 2225, 2234, 2237, 2987, 3080, 3197, 3484
Tsp45I GTSAC 7 cut(s) 329, 835, 1749, 1957, 2095, 3796, 4523
TspGWI ACGGA 1 cut(s) 3117
Vha464I CTTAAG 1 cut(s) 1517
VneI GTGCAC 3 cut(s) 197, 1396, 2832
VpaK11BI GGWCC 5 cut(s) 856, 1102, 1987, 2798, 3038
VspI ATTAAT 2 cut(s) 3984, 4964
XagI CCTNNNNNAGG 2 cut(s) 3844, 4280
XapI RAATTY 3 cut(s) 1198, 2635, 3012
XceI RCATGY 8 cut(s) 1030, 1134, 2037, 2075, 2473, 2808, 4493, 4817
XcmI CCANNNNNNNNNTGG 1 cut(s) 1377
XhoI CTCGAG 2 cut(s) 3521, 3892
XmiI GTMKAC 1 cut(s) 3551
XmnI GAANNNNTTC 2 cut(s) 905, 2648
XspI CTAG 5 cut(s) 474, 1205, 3750, 3885, 4409
ZraI GACGTC 2 cut(s) 752, 3917
ZrmI AGTACT 1 cut(s) 1892
Zsp2I ATGCAT 3 cut(s) 2194, 2471, 4560
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.