MD03G1134000.v1.1

Splicing factor U2af small subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0007614
Physical Location & Seq
Forward (+)
13390490 .. 13390651
162 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1134000.v1.1.491

Sequence Viewer

Length: 162 bp
ATGGCGAAGCATTTGGCTTCGATATTCGGGACGGAGAAGGACCGAGTGAACTGCCCTTTCTACTTCAAAATTGGGGCGTGTAGGCACAGCGACCGCTGCTCCAGACTTCACACCAAACCCAGCGTCAGCCCCACGATCTTGCTCTCCAACATGTACCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 ko05131 map03040 map05131
Pfam Domains
Protein Families
RRM_1 zf-CCCH

Protein Analysis

54

Amino Acids

6.06

Weight (kDa)

9.57

Isoelectric Point (pI)

50.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCCH PF00642 14 - 38 1.4e-07 Zinc finger C-x8-C-x5-C-x3-H type (and similar)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 94
AfaI GTAC 1 cut(s) 155
AflIII ACRYGT 1 cut(s) 150
AgsI TTSAA 1 cut(s) 67
ApeKI GCWGC 1 cut(s) 96
ArsI GACNNNNNNTTYG 2 cut(s) 108, 140
AspS9I GGNCC 1 cut(s) 40
AvaII GGWCC 1 cut(s) 40
BbvI GCAGC 1 cut(s) 83
BcgI CGANNNNNNTGC 2 cut(s) 33, 67
BisI GCNGC 1 cut(s) 97
BlsI GCNGC 1 cut(s) 98
Bme18I GGWCC 1 cut(s) 40
BmgT120I GGNCC 1 cut(s) 40
BpmI CTGGAG 1 cut(s) 85
BsaXI ACNNNNNCTCC 2 cut(s) 83, 113
Bse1I ACTGG 1 cut(s) 157
BseNI ACTGG 1 cut(s) 157
BseXI GCAGC 1 cut(s) 83
BseYI CCCAGC 1 cut(s) 119
Bsh1285I CGRYCG 1 cut(s) 94
BsiEI CGRYCG 1 cut(s) 94
BslFI GGGAC 1 cut(s) 43
BsmFI GGGAC 1 cut(s) 43
Bsp143I GATC 1 cut(s) 135
BspACI CCGC 1 cut(s) 94
BsrI ACTGG 1 cut(s) 157
BssMI GATC 1 cut(s) 135
BstKTI GATC 1 cut(s) 138
BstMBI GATC 1 cut(s) 135
BstMCI CGRYCG 1 cut(s) 94
BstMWI GCNNNNNNNGC 1 cut(s) 96
BstNSI RCATGY 1 cut(s) 154
BstV1I GCAGC 1 cut(s) 83
Cfr13I GGNCC 1 cut(s) 40
CseI GACGC 1 cut(s) 112
Csp6I GTAC 1 cut(s) 154
CviAII CATG 1 cut(s) 151
CviJI RGCY 2 cut(s) 17, 129
CviKI_1 RGCY 2 cut(s) 17, 129
CviQI GTAC 1 cut(s) 154
DpnI GATC 1 cut(s) 137
DpnII GATC 1 cut(s) 135
Eco47I GGWCC 1 cut(s) 40
FaeI CATG 1 cut(s) 154
FaiI YATR 1 cut(s) 152
FaqI GGGAC 1 cut(s) 43
FatI CATG 1 cut(s) 150
Fnu4HI GCNGC 1 cut(s) 97
Fsp4HI GCNGC 1 cut(s) 97
GluI GCNGC 1 cut(s) 97
GsaI CCCAGC 1 cut(s) 123
GsuI CTGGAG 1 cut(s) 85
HgaI GACGC 1 cut(s) 112
Hin1II CATG 1 cut(s) 154
Hpy166II GTNNAC 1 cut(s) 49
Hpy188III TCNNGA 2 cut(s) 28, 102
Hpy8I GTNNAC 1 cut(s) 49
HpyAV CCTTC 1 cut(s) 31
HpyF10VI GCNNNNNNNGC 1 cut(s) 96
Hsp92II CATG 1 cut(s) 154
Kzo9I GATC 1 cut(s) 135
LmnI GCTCC 1 cut(s) 104
LpnPI CCDG 2 cut(s) 115, 133
Lsp1109I GCAGC 1 cut(s) 83
MalI GATC 1 cut(s) 137
MboI GATC 1 cut(s) 135
MluCI AATT 1 cut(s) 69
MspA1I CMGCKG 1 cut(s) 96
MwoI GCNNNNNNNGC 1 cut(s) 96
NdeII GATC 1 cut(s) 135
NlaIII CATG 1 cut(s) 154
NspI RCATGY 1 cut(s) 154
PciI ACATGT 1 cut(s) 150
PkrI GCNGC 1 cut(s) 98
PscI ACATGT 1 cut(s) 150
PspFI CCCAGC 1 cut(s) 119
PspPI GGNCC 1 cut(s) 40
RsaI GTAC 1 cut(s) 155
RsaNI GTAC 1 cut(s) 154
SatI GCNGC 1 cut(s) 97
Sau3AI GATC 1 cut(s) 135
Sau96I GGNCC 1 cut(s) 40
SgeI CNNG 7 cut(s) 40, 56, 90, 114, 132, 145, 151
SinI GGWCC 1 cut(s) 40
Sse9I AATT 1 cut(s) 69
SsiI CCGC 1 cut(s) 94
TaqI TCGA 1 cut(s) 20
TaqII GACCGA 1 cut(s) 57
TasI AATT 1 cut(s) 69
TseI GCWGC 1 cut(s) 96
TspGWI ACGGA 1 cut(s) 47
VpaK11BI GGWCC 1 cut(s) 40
XceI RCATGY 1 cut(s) 154
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.