MD03G1135500.v1.1

Pentatricopeptide repeat-containing protein At2g27800

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0012956
Physical Location & Seq
Forward (+)
13562313 .. 13563878
1566 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1135500.v1.1.491

Sequence Viewer

Length: 276 bp
ATGTCAGCTTCGATCGCCGGAAGATCGACAATTCTGCGCACGTTTTGCCAAGCAGCAACATCAACAACAAGAACAAGAATTTCAGCAGCGCCATGCCCTTCGATTCCGTCCAATTTCATGGCTCGTCGCTCCTCTTCCTCAAGGCTGTTGGGCCGAGAATTGAGCTCTCTTCAACCTCTCCACAATGCCATTGCGTCCGCTTGCCTTATCTCCAAGCTTCCGGCCCTGGCCACCTCCTCCGAAGGCAGATTTGTGAACTATATCAGTCCTATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

92

Amino Acids

9.63

Weight (kDa)

11.48

Isoelectric Point (pI)

80.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 38
AciI CCGC 1 cut(s) 198
AcoI YGGCCR 1 cut(s) 228
AcsI RAATTY 1 cut(s) 78
AgsI TTSAA 1 cut(s) 173
AjnI CCWGG 1 cut(s) 225
AluBI AGCT 3 cut(s) 8, 165, 217
AluI AGCT 3 cut(s) 8, 165, 217
Alw21I GWGCWC 1 cut(s) 167
AoxI GGCC 3 cut(s) 151, 222, 228
ApeKI GCWGC 2 cut(s) 53, 86
ApoI RAATTY 1 cut(s) 78
AspLEI GCGC 2 cut(s) 39, 91
AspS9I GGNCC 2 cut(s) 151, 223
BalI TGGCCA 1 cut(s) 230
BanII GRGCYC 1 cut(s) 167
Bbv12I GWGCWC 1 cut(s) 167
BbvI GCAGC 2 cut(s) 65, 98
BcgI CGANNNNNNTGC 2 cut(s) 16, 50
BciT130I CCWGG 1 cut(s) 227
BfaI CTAG 1 cut(s) 274
BfoI RGCGCY 1 cut(s) 92
BisI GCNGC 2 cut(s) 54, 87
BlsI GCNGC 2 cut(s) 55, 88
Bme1390I CCNGG 1 cut(s) 227
BmgT120I GGNCC 2 cut(s) 151, 223
BmrFI CCNGG 1 cut(s) 227
BpuEI CTTGAG 1 cut(s) 124
BsaJI CCNNGG 1 cut(s) 225
Bse3DI GCAATG 1 cut(s) 189
BseBI CCWGG 1 cut(s) 227
BseDI CCNNGG 1 cut(s) 225
BseMI GCAATG 1 cut(s) 189
BseRI GAGGAG 2 cut(s) 121, 226
BseXI GCAGC 2 cut(s) 65, 98
Bsh1285I CGRYCG 1 cut(s) 15
BshFI GGCC 3 cut(s) 153, 224, 230
BsiEI CGRYCG 1 cut(s) 15
BsiHKAI GWGCWC 1 cut(s) 167
BsiSI CCGG 2 cut(s) 18, 221
BsnI GGCC 3 cut(s) 153, 224, 230
Bsp1286I GDGCHC 1 cut(s) 167
Bsp143I GATC 2 cut(s) 12, 23
BspACI CCGC 1 cut(s) 198
BspANI GGCC 3 cut(s) 153, 224, 230
BsrDI GCAATG 1 cut(s) 189
BssECI CCNNGG 1 cut(s) 225
BssMI GATC 2 cut(s) 12, 23
Bst2UI CCWGG 1 cut(s) 227
Bst6I CTCTTC 2 cut(s) 139, 174
BstAPI GCANNNNNTGC 1 cut(s) 45
BstC8I GCNNGC 1 cut(s) 202
BstH2I RGCGCY 1 cut(s) 92
BstHHI GCGC 2 cut(s) 39, 91
BstKTI GATC 2 cut(s) 15, 26
BstMBI GATC 2 cut(s) 12, 23
BstMCI CGRYCG 1 cut(s) 15
BstMWI GCNNNNNNNGC 2 cut(s) 14, 45
BstNI CCWGG 1 cut(s) 227
BstSCI CCNGG 1 cut(s) 225
BstV1I GCAGC 2 cut(s) 65, 98
BstXI CCANNNNNNTGG 1 cut(s) 118
BsuRI GGCC 3 cut(s) 153, 224, 230
Cac8I GCNNGC 1 cut(s) 202
CfoI GCGC 2 cut(s) 39, 91
Cfr13I GGNCC 2 cut(s) 151, 223
CseI GACGC 1 cut(s) 183
CviAII CATG 2 cut(s) 93, 118
CviJI RGCY 8 cut(s) 8, 122, 145, 153, 165, 217, 224, 230
CviKI_1 RGCY 8 cut(s) 8, 122, 145, 153, 165, 217, 224, 230
DpnI GATC 2 cut(s) 14, 25
DpnII GATC 2 cut(s) 12, 23
EaeI YGGCCR 1 cut(s) 228
Eam1104I CTCTTC 2 cut(s) 139, 174
EarI CTCTTC 2 cut(s) 139, 174
Ecl136II GAGCTC 1 cut(s) 165
Eco24I GRGCYC 1 cut(s) 167
Eco53kI GAGCTC 1 cut(s) 165
EcoICRI GAGCTC 1 cut(s) 165
EcoRII CCWGG 1 cut(s) 225
EcoT38I GRGCYC 1 cut(s) 167
FaeI CATG 2 cut(s) 96, 121
FaiI YATR 3 cut(s) 94, 119, 261
FatI CATG 2 cut(s) 92, 117
Fnu4HI GCNGC 2 cut(s) 54, 87
FriOI GRGCYC 1 cut(s) 167
Fsp4HI GCNGC 2 cut(s) 54, 87
FspBI CTAG 1 cut(s) 274
FspI TGCGCA 1 cut(s) 38
GlaI GCGC 2 cut(s) 38, 90
GluI GCNGC 2 cut(s) 54, 87
HaeII RGCGCY 1 cut(s) 92
HaeIII GGCC 3 cut(s) 153, 224, 230
HapII CCGG 2 cut(s) 18, 221
HgaI GACGC 1 cut(s) 183
HhaI GCGC 2 cut(s) 39, 91
Hin1II CATG 2 cut(s) 96, 121
Hin6I GCGC 2 cut(s) 37, 89
HinP1I GCGC 2 cut(s) 37, 89
HindIII AAGCTT 1 cut(s) 215
HinfI GANTC 1 cut(s) 103
HpaII CCGG 2 cut(s) 18, 221
Hpy166II GTNNAC 1 cut(s) 256
Hpy188I TCNGA 1 cut(s) 241
Hpy8I GTNNAC 1 cut(s) 256
Hpy99I CGWCG 1 cut(s) 129
HpyAV CCTTC 2 cut(s) 108, 236
HpyCH4IV ACGT 1 cut(s) 41
HpyF10VI GCNNNNNNNGC 2 cut(s) 14, 45
HpySE526I ACGT 1 cut(s) 41
Hsp92II CATG 2 cut(s) 96, 121
HspAI GCGC 2 cut(s) 37, 89
Kzo9I GATC 2 cut(s) 12, 23
LmnI GCTCC 1 cut(s) 134
LpnPI CCDG 4 cut(s) 31, 212, 234, 239
Lsp1109I GCAGC 2 cut(s) 65, 98
MaeI CTAG 1 cut(s) 274
MaeII ACGT 1 cut(s) 41
MalI GATC 2 cut(s) 14, 25
MboI GATC 2 cut(s) 12, 23
MboII GAAGA 3 cut(s) 33, 126, 161
MhlI GDGCHC 1 cut(s) 167
MlsI TGGCCA 1 cut(s) 230
MluCI AATT 4 cut(s) 30, 78, 112, 158
MluNI TGGCCA 1 cut(s) 230
MnlI CCTC 5 cut(s) 142, 148, 186, 244, 247
Mox20I TGGCCA 1 cut(s) 230
MscI TGGCCA 1 cut(s) 230
Msp20I TGGCCA 1 cut(s) 230
MspI CCGG 2 cut(s) 18, 221
MspR9I CCNGG 1 cut(s) 227
MvaI CCWGG 1 cut(s) 227
MwoI GCNNNNNNNGC 2 cut(s) 14, 45
NdeII GATC 2 cut(s) 12, 23
NlaIII CATG 2 cut(s) 96, 121
NmeAIII GCCGAG 1 cut(s) 179
NsbI TGCGCA 1 cut(s) 38
PfeI GAWTC 1 cut(s) 103
PkrI GCNGC 2 cut(s) 55, 88
Ple19I CGATCG 1 cut(s) 15
Psp124BI GAGCTC 1 cut(s) 167
Psp6I CCWGG 1 cut(s) 225
PspGI CCWGG 1 cut(s) 225
PspPI GGNCC 2 cut(s) 151, 223
PvuI CGATCG 1 cut(s) 15
SacI GAGCTC 1 cut(s) 167
SatI GCNGC 2 cut(s) 54, 87
Sau3AI GATC 2 cut(s) 12, 23
Sau96I GGNCC 2 cut(s) 151, 223
ScrFI CCNGG 1 cut(s) 227
SduI GDGCHC 1 cut(s) 167
SetI ASST 6 cut(s) 10, 44, 167, 178, 219, 236
SmlI CTYRAG 1 cut(s) 139
SmoI CTYRAG 1 cut(s) 139
Sse9I AATT 4 cut(s) 30, 78, 112, 158
SsiI CCGC 1 cut(s) 198
SspMI CTAG 1 cut(s) 274
SstI GAGCTC 1 cut(s) 167
StyD4I CCNGG 1 cut(s) 225
TaiI ACGT 1 cut(s) 44
TaqI TCGA 3 cut(s) 11, 26, 101
TasI AATT 4 cut(s) 30, 78, 112, 158
TfiI GAWTC 1 cut(s) 103
TseI GCWGC 2 cut(s) 53, 86
TspDTI ATGAA 1 cut(s) 106
TspGWI ACGGA 1 cut(s) 96
XapI RAATTY 1 cut(s) 78
XspI CTAG 1 cut(s) 274
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.