MD11G1158100.v1.1

Pentatricopeptide repeat-containing protein At2g27800

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0012956
Physical Location & Seq
Forward (+)
15368349 .. 15369908
1560 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1158100.v1.1.491

Sequence Viewer

Length: 276 bp
ATGTCAGCTTCGATCGCTGGAAGATCGACAATTCTGCGCGCGTTTTTCCGAGCAGCGACGTCAACAGCAAGAACAAGAATTTCAGCAGCGCCATGCCCTTCGATTCCGTCCAATTTCATGGCTCGTCGCTCCCCTTCCTCAAGGCTGTTGCGGCGTGAATTGAGCTCTCTTCAACCGCTCCACACTGCCATTGCGTCCGCTTGCCTTATCTCCAAGCTCCCCGCCCTGGCCACCTCCTCCGAAGGTAGATTTGTGAATTACATCAGTCCCATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

92

Amino Acids

9.74

Weight (kDa)

11.92

Isoelectric Point (pI)

87.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 62
AccBSI CCGCTC 1 cut(s) 178
AccII CGCG 2 cut(s) 39, 41
AciI CCGC 4 cut(s) 151, 176, 198, 222
AcoI YGGCCR 1 cut(s) 228
AcsI RAATTY 1 cut(s) 78
AcyI GRCGYC 1 cut(s) 59
AfiI CCNNNNNNNGG 1 cut(s) 226
AgsI TTSAA 1 cut(s) 173
AjnI CCWGG 1 cut(s) 225
AluBI AGCT 3 cut(s) 8, 165, 217
AluI AGCT 3 cut(s) 8, 165, 217
Alw21I GWGCWC 1 cut(s) 167
AoxI GGCC 1 cut(s) 228
ApeKI GCWGC 2 cut(s) 53, 86
ApoI RAATTY 1 cut(s) 78
AspLEI GCGC 3 cut(s) 39, 41, 91
BalI TGGCCA 1 cut(s) 230
BanII GRGCYC 1 cut(s) 167
Bbv12I GWGCWC 1 cut(s) 167
BbvI GCAGC 2 cut(s) 65, 98
BcgI CGANNNNNNTGC 2 cut(s) 16, 50
BciT130I CCWGG 1 cut(s) 227
BfaI CTAG 1 cut(s) 274
BfoI RGCGCY 1 cut(s) 92
BisI GCNGC 3 cut(s) 54, 87, 152
BlsI GCNGC 3 cut(s) 55, 88, 153
Bme1390I CCNGG 1 cut(s) 227
BmrFI CCNGG 1 cut(s) 227
BpuEI CTTGAG 1 cut(s) 124
BsaHI GRCGYC 1 cut(s) 59
BsaJI CCNNGG 1 cut(s) 225
Bsc4I CCNNNNNNNGG 1 cut(s) 226
Bse3DI GCAATG 1 cut(s) 189
BseBI CCWGG 1 cut(s) 227
BseDI CCNNGG 1 cut(s) 225
BseLI CCNNNNNNNGG 1 cut(s) 226
BseMI GCAATG 1 cut(s) 189
BsePI GCGCGC 1 cut(s) 37
BseRI GAGGAG 1 cut(s) 226
BseXI GCAGC 2 cut(s) 65, 98
Bsh1236I CGCG 2 cut(s) 39, 41
Bsh1285I CGRYCG 1 cut(s) 15
BshFI GGCC 1 cut(s) 230
BsiEI CGRYCG 1 cut(s) 15
BsiHKAI GWGCWC 1 cut(s) 167
BslFI GGGAC 1 cut(s) 252
BslI CCNNNNNNNGG 1 cut(s) 226
BsmFI GGGAC 1 cut(s) 252
BsnI GGCC 1 cut(s) 230
Bsp1286I GDGCHC 1 cut(s) 167
Bsp143I GATC 2 cut(s) 12, 23
BspACI CCGC 4 cut(s) 151, 176, 198, 222
BspANI GGCC 1 cut(s) 230
BspFNI CGCG 2 cut(s) 39, 41
BsrBI CCGCTC 1 cut(s) 178
BsrDI GCAATG 1 cut(s) 189
BssECI CCNNGG 1 cut(s) 225
BssHII GCGCGC 1 cut(s) 37
BssMI GATC 2 cut(s) 12, 23
BssNI GRCGYC 1 cut(s) 59
Bst2UI CCWGG 1 cut(s) 227
Bst6I CTCTTC 1 cut(s) 174
BstACI GRCGYC 1 cut(s) 59
BstC8I GCNNGC 2 cut(s) 39, 202
BstFNI CGCG 2 cut(s) 39, 41
BstH2I RGCGCY 1 cut(s) 92
BstHHI GCGC 3 cut(s) 39, 41, 91
BstKTI GATC 2 cut(s) 15, 26
BstMBI GATC 2 cut(s) 12, 23
BstMCI CGRYCG 1 cut(s) 15
BstMWI GCNNNNNNNGC 2 cut(s) 14, 151
BstNI CCWGG 1 cut(s) 227
BstSCI CCNGG 1 cut(s) 225
BstUI CGCG 2 cut(s) 39, 41
BstV1I GCAGC 2 cut(s) 65, 98
BstXI CCANNNNNNTGG 1 cut(s) 118
BsuRI GGCC 1 cut(s) 230
BtsI GCAGTG 1 cut(s) 183
BtsIMutI CAGTG 1 cut(s) 183
Cac8I GCNNGC 2 cut(s) 39, 202
CfoI GCGC 3 cut(s) 39, 41, 91
CseI GACGC 1 cut(s) 183
CviAII CATG 2 cut(s) 93, 118
CviJI RGCY 6 cut(s) 8, 122, 145, 165, 217, 230
CviKI_1 RGCY 6 cut(s) 8, 122, 145, 165, 217, 230
DpnI GATC 2 cut(s) 14, 25
DpnII GATC 2 cut(s) 12, 23
EaeI YGGCCR 1 cut(s) 228
Eam1104I CTCTTC 1 cut(s) 174
EarI CTCTTC 1 cut(s) 174
Ecl136II GAGCTC 1 cut(s) 165
Eco24I GRGCYC 1 cut(s) 167
Eco53kI GAGCTC 1 cut(s) 165
EcoICRI GAGCTC 1 cut(s) 165
EcoRII CCWGG 1 cut(s) 225
EcoT38I GRGCYC 1 cut(s) 167
FaeI CATG 2 cut(s) 96, 121
FaiI YATR 2 cut(s) 94, 119
FaqI GGGAC 1 cut(s) 252
FatI CATG 2 cut(s) 92, 117
FauI CCCGC 1 cut(s) 229
Fnu4HI GCNGC 3 cut(s) 54, 87, 152
FriOI GRGCYC 1 cut(s) 167
Fsp4HI GCNGC 3 cut(s) 54, 87, 152
FspBI CTAG 1 cut(s) 274
GlaI GCGC 3 cut(s) 38, 40, 90
GluI GCNGC 3 cut(s) 54, 87, 152
HaeII RGCGCY 1 cut(s) 92
HaeIII GGCC 1 cut(s) 230
HgaI GACGC 1 cut(s) 183
HhaI GCGC 3 cut(s) 39, 41, 91
Hin1I GRCGYC 1 cut(s) 59
Hin1II CATG 2 cut(s) 96, 121
Hin6I GCGC 3 cut(s) 37, 39, 89
HinP1I GCGC 3 cut(s) 37, 39, 89
HincII GTYRAC 1 cut(s) 63
HindII GTYRAC 1 cut(s) 63
HinfI GANTC 1 cut(s) 103
Hpy166II GTNNAC 1 cut(s) 63
Hpy188I TCNGA 2 cut(s) 50, 241
Hpy8I GTNNAC 1 cut(s) 63
Hpy99I CGWCG 2 cut(s) 61, 129
HpyAV CCTTC 3 cut(s) 108, 144, 236
HpyCH4IV ACGT 1 cut(s) 59
HpyF10VI GCNNNNNNNGC 2 cut(s) 14, 151
HpySE526I ACGT 1 cut(s) 59
Hsp92I GRCGYC 1 cut(s) 59
Hsp92II CATG 2 cut(s) 96, 121
HspAI GCGC 3 cut(s) 37, 39, 89
Kzo9I GATC 2 cut(s) 12, 23
LmnI GCTCC 3 cut(s) 134, 183, 222
LpnPI CCDG 3 cut(s) 3, 212, 239
Lsp1109I GCAGC 2 cut(s) 65, 98
MaeI CTAG 1 cut(s) 274
MaeII ACGT 1 cut(s) 59
MalI GATC 2 cut(s) 14, 25
MbiI CCGCTC 1 cut(s) 178
MboI GATC 2 cut(s) 12, 23
MboII GAAGA 2 cut(s) 33, 161
MhlI GDGCHC 1 cut(s) 167
MlsI TGGCCA 1 cut(s) 230
MluCI AATT 5 cut(s) 30, 78, 112, 158, 256
MluNI TGGCCA 1 cut(s) 230
MnlI CCTC 3 cut(s) 148, 244, 247
Mox20I TGGCCA 1 cut(s) 230
MscI TGGCCA 1 cut(s) 230
Msp20I TGGCCA 1 cut(s) 230
MspR9I CCNGG 1 cut(s) 227
MvaI CCWGG 1 cut(s) 227
MvnI CGCG 2 cut(s) 39, 41
MwoI GCNNNNNNNGC 2 cut(s) 14, 151
NdeII GATC 2 cut(s) 12, 23
NlaIII CATG 2 cut(s) 96, 121
PauI GCGCGC 1 cut(s) 37
PfeI GAWTC 1 cut(s) 103
PkrI GCNGC 3 cut(s) 55, 88, 153
Ple19I CGATCG 1 cut(s) 15
Psp124BI GAGCTC 1 cut(s) 167
Psp6I CCWGG 1 cut(s) 225
PspGI CCWGG 1 cut(s) 225
PteI GCGCGC 1 cut(s) 37
PvuI CGATCG 1 cut(s) 15
SacI GAGCTC 1 cut(s) 167
SatI GCNGC 3 cut(s) 54, 87, 152
Sau3AI GATC 2 cut(s) 12, 23
ScrFI CCNGG 1 cut(s) 227
SduI GDGCHC 1 cut(s) 167
SetI ASST 6 cut(s) 10, 62, 167, 219, 236, 247
SmlI CTYRAG 1 cut(s) 139
SmoI CTYRAG 1 cut(s) 139
Sse9I AATT 5 cut(s) 30, 78, 112, 158, 256
SsiI CCGC 4 cut(s) 151, 176, 198, 222
SspMI CTAG 1 cut(s) 274
SstI GAGCTC 1 cut(s) 167
StyD4I CCNGG 1 cut(s) 225
TaiI ACGT 1 cut(s) 62
TaqI TCGA 3 cut(s) 11, 26, 101
TasI AATT 5 cut(s) 30, 78, 112, 158, 256
TauI GCSGC 1 cut(s) 154
TfiI GAWTC 1 cut(s) 103
TscAI CASTG 1 cut(s) 190
TseI GCWGC 2 cut(s) 53, 86
TspDTI ATGAA 1 cut(s) 106
TspGWI ACGGA 1 cut(s) 96
TspRI CASTG 1 cut(s) 190
XapI RAATTY 1 cut(s) 78
XspI CTAG 1 cut(s) 274
ZraI GACGTC 1 cut(s) 60
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.