MD03G1136300.v1.1

Transcriptional activator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0008252
Physical Location & Seq
Reverse (-)
13682385 .. 13683889
1505 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1136300.v1.1.491

Sequence Viewer

Length: 519 bp
ATGGCGACGCCGAAACAGATGTGGGAGCAGCAGCAATTGCAGGTGCAGCTAGTGAAGAACTCCGGTATCGTCAGTTACAATGGGAGTCCGGTAGCTGATGACAAGGAAGAAGAGATGTCAAGGTCTGCATTGGCTTTGTTCAGGGCAAAGGAGGAAGAGATTGAGAGGAAGAAGTTGGAGGTGAAAGACAAGGTTCAGGCTCAGCTGGGACGCGTCGAGGAAGCAACTAAGCGCTTGTCTGAGATTCAGGAAGAGCTTGAAACGCTGATAGATCCAATGAGAAAGGAAGTTGCAATTATTCGCAAGAGGATAGACATCATTAACAAAGAGTTAAAGCCGCTAGGTCAGAGCTGCCAGAGGAAGGAGAAAGAATACAAGGAAGCTCTCGAGGCTTTAAACGAGAAGAGCAGAGAGAAATCTCAACTAGTTTCCAAATTGATGGAGCTTGTGGGTGAAAGTGAGAGAATGAGGATAAAGAAGCTGGAGGAGCTGAACAAACATGTTGAAAATCTGCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04115 map04115
Pfam Domains
Protein Families
Transcrip_act

Protein Analysis

173

Amino Acids

20.11

Weight (kDa)

7.74

Isoelectric Point (pI)

53.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transcrip_act PF04949 19 - 169 1.8e-71 Transcriptional activator
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 31
Acc36I ACCTGC 1 cut(s) 31
AccII CGCG 1 cut(s) 213
AciI CCGC 1 cut(s) 338
AclWI GGATC 1 cut(s) 266
AcyI GRCGYC 1 cut(s) 8
AfeI AGCGCT 1 cut(s) 233
AfiI CCNNNNNNNGG 1 cut(s) 361
AflIII ACRYGT 2 cut(s) 211, 499
AgsI TTSAA 2 cut(s) 260, 506
AhlI ACTAGT 1 cut(s) 424
AluBI AGCT 9 cut(s) 49, 95, 205, 256, 351, 383, 445, 481, 490
AluI AGCT 9 cut(s) 49, 95, 205, 256, 351, 383, 445, 481, 490
AlwI GGATC 1 cut(s) 266
Ama87I CYCGRG 1 cut(s) 386
Aor51HI AGCGCT 1 cut(s) 233
ApeKI GCWGC 4 cut(s) 28, 31, 46, 351
AspLEI GCGC 1 cut(s) 234
AsuHPI GGTGA 2 cut(s) 193, 464
AvaI CYCGRG 1 cut(s) 386
BaeI ACNNNNGTAYC 2 cut(s) 49, 82
BbvI GCAGC 4 cut(s) 40, 43, 58, 338
BccI CCATC 1 cut(s) 433
BcuI ACTAGT 1 cut(s) 424
BfaI CTAG 3 cut(s) 50, 341, 425
BfoI RGCGCY 1 cut(s) 235
BfuAI ACCTGC 1 cut(s) 31
BisI GCNGC 5 cut(s) 29, 32, 47, 338, 352
BlpI GCTNAGC 1 cut(s) 201
BlsI GCNGC 5 cut(s) 30, 33, 48, 339, 353
BmeT110I CYCGRG 1 cut(s) 386
BpmI CTGGAG 1 cut(s) 503
Bpu1102I GCTNAGC 1 cut(s) 201
BsaBI GATNNNNATC 1 cut(s) 314
BsaHI GRCGYC 1 cut(s) 8
BsaWI WCCGGW 2 cut(s) 62, 88
Bsc4I CCNNNNNNNGG 1 cut(s) 361
Bse8I GATNNNNATC 1 cut(s) 314
BseJI GATNNNNATC 1 cut(s) 314
BseLI CCNNNNNNNGG 1 cut(s) 361
BseMII CTCAG 2 cut(s) 215, 231
BseRI GAGGAG 1 cut(s) 500
BseXI GCAGC 4 cut(s) 40, 43, 58, 338
BseYI CCCAGC 1 cut(s) 205
BsgI GTGCAG 1 cut(s) 65
Bsh1236I CGCG 1 cut(s) 213
BsiHKCI CYCGRG 1 cut(s) 386
BsiSI CCGG 2 cut(s) 63, 89
BslFI GGGAC 1 cut(s) 222
BslI CCNNNNNNNGG 1 cut(s) 361
BsmFI GGGAC 1 cut(s) 222
BsoBI CYCGRG 1 cut(s) 386
Bsp143I GATC 1 cut(s) 271
Bsp1720I GCTNAGC 1 cut(s) 201
BspACI CCGC 1 cut(s) 338
BspCNI CTCAG 2 cut(s) 214, 232
BspFNI CGCG 1 cut(s) 213
BspMI ACCTGC 1 cut(s) 31
BspPI GGATC 1 cut(s) 266
BspQI GCTCTTC 2 cut(s) 246, 398
BssMI GATC 1 cut(s) 271
BssNI GRCGYC 1 cut(s) 8
Bst6I CTCTTC 4 cut(s) 105, 150, 246, 398
BstACI GRCGYC 1 cut(s) 8
BstAPI GCANNNNNTGC 1 cut(s) 37
BstDEI CTNAG 3 cut(s) 201, 228, 240
BstFNI CGCG 1 cut(s) 213
BstH2I RGCGCY 1 cut(s) 235
BstHHI GCGC 1 cut(s) 234
BstKTI GATC 1 cut(s) 274
BstMBI GATC 1 cut(s) 271
BstMWI GCNNNNNNNGC 5 cut(s) 37, 46, 262, 389, 487
BstNSI RCATGY 1 cut(s) 503
BstUI CGCG 1 cut(s) 213
BstV1I GCAGC 4 cut(s) 40, 43, 58, 338
BstX2I RGATCY 1 cut(s) 271
BstXI CCANNNNNNTGG 1 cut(s) 439
BstYI RGATCY 1 cut(s) 271
BveI ACCTGC 1 cut(s) 31
CfoI GCGC 1 cut(s) 234
CseI GACGC 3 cut(s) 16, 202, 219
CviAII CATG 1 cut(s) 500
DdeI CTNAG 3 cut(s) 201, 228, 240
DpnI GATC 1 cut(s) 273
DpnII GATC 1 cut(s) 271
DraI TTTAAA 1 cut(s) 396
Eam1104I CTCTTC 4 cut(s) 105, 150, 246, 398
EarI CTCTTC 4 cut(s) 105, 150, 246, 398
Eco47III AGCGCT 1 cut(s) 233
Eco88I CYCGRG 1 cut(s) 386
FaeI CATG 1 cut(s) 503
FaiI YATR 1 cut(s) 501
FaqI GGGAC 1 cut(s) 222
FatI CATG 1 cut(s) 499
Fnu4HI GCNGC 5 cut(s) 29, 32, 47, 338, 352
Fsp4HI GCNGC 5 cut(s) 29, 32, 47, 338, 352
FspBI CTAG 3 cut(s) 50, 341, 425
GlaI GCGC 1 cut(s) 233
GluI GCNGC 5 cut(s) 29, 32, 47, 338, 352
GsaI CCCAGC 1 cut(s) 209
GsuI CTGGAG 1 cut(s) 503
HaeII RGCGCY 1 cut(s) 235
HapII CCGG 2 cut(s) 63, 89
HgaI GACGC 3 cut(s) 16, 202, 219
HhaI GCGC 1 cut(s) 234
Hin1I GRCGYC 1 cut(s) 8
Hin1II CATG 1 cut(s) 503
Hin6I GCGC 1 cut(s) 232
HinP1I GCGC 1 cut(s) 232
HinfI GANTC 2 cut(s) 85, 244
HpaII CCGG 2 cut(s) 63, 89
HphI GGTGA 2 cut(s) 193, 464
Hpy188I TCNGA 2 cut(s) 241, 348
Hpy188III TCNNGA 2 cut(s) 248, 386
Hpy99I CGWCG 2 cut(s) 10, 218
HpyAV CCTTC 1 cut(s) 355
HpyCH4V TGCA 4 cut(s) 40, 46, 128, 293
HpyF10VI GCNNNNNNNGC 5 cut(s) 37, 46, 262, 389, 487
HpyF3I CTNAG 3 cut(s) 201, 228, 240
Hsp92I GRCGYC 1 cut(s) 8
Hsp92II CATG 1 cut(s) 503
HspAI GCGC 1 cut(s) 232
Kzo9I GATC 1 cut(s) 271
LguI GCTCTTC 2 cut(s) 246, 398
LmnI GCTCC 3 cut(s) 25, 442, 487
LpnPI CCDG 9 cut(s) 26, 76, 102, 127, 182, 191, 233, 368, 467
Lsp1109I GCAGC 4 cut(s) 40, 43, 58, 338
MaeI CTAG 3 cut(s) 50, 341, 425
MaeIII GTNAC 1 cut(s) 74
MalI GATC 1 cut(s) 273
MboI GATC 1 cut(s) 271
MboII GAAGA 7 cut(s) 67, 119, 122, 167, 181, 263, 415
MfeI CAATTG 1 cut(s) 35
MflI RGATCY 1 cut(s) 271
MluCI AATT 3 cut(s) 35, 294, 434
MluI ACGCGT 1 cut(s) 211
MlyI GAGTC 1 cut(s) 94
MmeI TCCRAC 1 cut(s) 156
MnlI CCTC 9 cut(s) 145, 159, 172, 211, 300, 351, 382, 462, 478
MseI TTAA 4 cut(s) 321, 332, 395, 517
MspA1I CMGCKG 1 cut(s) 205
MspI CCGG 2 cut(s) 63, 89
MunI CAATTG 1 cut(s) 35
MvnI CGCG 1 cut(s) 213
MwoI GCNNNNNNNGC 5 cut(s) 37, 46, 262, 389, 487
NdeII GATC 1 cut(s) 271
NlaIII CATG 1 cut(s) 503
NspI RCATGY 1 cut(s) 503
PaeR7I CTCGAG 1 cut(s) 386
PaqCI CACCTGC 1 cut(s) 31
PciI ACATGT 1 cut(s) 499
PciSI GCTCTTC 2 cut(s) 246, 398
PfeI GAWTC 1 cut(s) 244
PkrI GCNGC 5 cut(s) 30, 33, 48, 339, 353
PleI GAGTC 1 cut(s) 93
PpsI GAGTC 1 cut(s) 93
PscI ACATGT 1 cut(s) 499
PspFI CCCAGC 1 cut(s) 205
PsuI RGATCY 1 cut(s) 271
PvuII CAGCTG 1 cut(s) 205
SapI GCTCTTC 2 cut(s) 246, 398
SaqAI TTAA 4 cut(s) 321, 332, 395, 517
SatI GCNGC 5 cut(s) 29, 32, 47, 338, 352
Sau3AI GATC 1 cut(s) 271
SchI GAGTC 1 cut(s) 94
Sfr274I CTCGAG 1 cut(s) 386
SlaI CTCGAG 1 cut(s) 386
SmlI CTYRAG 1 cut(s) 386
SmoI CTYRAG 1 cut(s) 386
SpeI ACTAGT 1 cut(s) 424
Sse9I AATT 3 cut(s) 35, 294, 434
SsiI CCGC 1 cut(s) 338
SspMI CTAG 3 cut(s) 50, 341, 425
TaqI TCGA 2 cut(s) 216, 387
TasI AATT 3 cut(s) 35, 294, 434
TauI GCSGC 1 cut(s) 340
TfiI GAWTC 1 cut(s) 244
Tru1I TTAA 4 cut(s) 321, 332, 395, 517
Tru9I TTAA 4 cut(s) 321, 332, 395, 517
TseI GCWGC 4 cut(s) 28, 31, 46, 351
XceI RCATGY 1 cut(s) 503
XhoI CTCGAG 1 cut(s) 386
XspI CTAG 3 cut(s) 50, 341, 425
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.