MD11G1158600.v1.1

Transcriptional activator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0008252
Physical Location & Seq
Reverse (-)
15435751 .. 15437571
1821 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1158600.v1.1.491

Sequence Viewer

Length: 519 bp
ATGGCGACGCCGAAACAGATGTGGGAACAACAGCAATTGCAGGTGCAGCGAGTGAAGAACTCCGGTATCATCAGTTACAATGGGAGTCCAGTAGCTGATGATAAGGAAGAAGAAATGTCAAGGTCTGCATTGGCTTTGTTCCGGGCAAAGGAGGAAGAGATTGAGAAGAAGAAGTCGGAGGTGAAAGACAAGGTTCAGGCTCAGTTGGGACGCGTTGAAGAAGCAACTAAACGCTTGTCTGAGATTCAGGATGAGCTTGAAGCGCTAACAGATCCAATGAGAAAGGAACTTGCAATTATTCGCAAGAGGATAGACGTTATTCACAAAGAGTTAAAGCCGCTAGGCCTGAGCTGCCAAAGGAAGGAGAAAGAATACAAGGAAGCTCTTGAGGCTTTAAACGAGAAAAGCAGAGAGAAATCTCAACTAGTTTCCAAATTGATGGAGCTTGTGGGTGAAAGTGAGAGAATGAGGATGAAGAAGCTGGAGGAGCTGAACAAACATTTCGAAACTCTGCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04115 map04115
Pfam Domains
Protein Families
Transcrip_act

Protein Analysis

173

Amino Acids

20.12

Weight (kDa)

8.51

Isoelectric Point (pI)

52.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transcrip_act PF04949 19 - 170 2.2e-69 Transcriptional activator
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 31
Acc36I ACCTGC 1 cut(s) 31
AccII CGCG 1 cut(s) 213
AciI CCGC 1 cut(s) 338
AclWI GGATC 1 cut(s) 266
AcyI GRCGYC 1 cut(s) 8
AfeI AGCGCT 1 cut(s) 264
AfiI CCNNNNNNNGG 2 cut(s) 148, 361
AflIII ACRYGT 1 cut(s) 211
AgsI TTSAA 2 cut(s) 218, 260
AhlI ACTAGT 1 cut(s) 424
AluBI AGCT 7 cut(s) 95, 256, 351, 383, 445, 481, 490
AluI AGCT 7 cut(s) 95, 256, 351, 383, 445, 481, 490
AlwI GGATC 1 cut(s) 266
AlwNI CAGNNNCTG 1 cut(s) 95
Aor51HI AGCGCT 1 cut(s) 264
AoxI GGCC 1 cut(s) 343
ApeKI GCWGC 2 cut(s) 46, 351
AspLEI GCGC 1 cut(s) 265
AsuC2I CCSGG 1 cut(s) 143
AsuHPI GGTGA 2 cut(s) 193, 464
AsuII TTCGAA 1 cut(s) 504
BaeI ACNNNNGTAYC 2 cut(s) 49, 82
BbvI GCAGC 2 cut(s) 58, 338
BccI CCATC 1 cut(s) 433
BcnI CCSGG 1 cut(s) 143
BcuI ACTAGT 1 cut(s) 424
BfaI CTAG 2 cut(s) 341, 425
BfoI RGCGCY 1 cut(s) 266
BfuAI ACCTGC 1 cut(s) 31
BisI GCNGC 3 cut(s) 47, 338, 352
BlsI GCNGC 3 cut(s) 48, 339, 353
Bme1390I CCNGG 1 cut(s) 143
BmrFI CCNGG 1 cut(s) 143
BpmI CTGGAG 1 cut(s) 503
Bpu10I CCTNAGC 1 cut(s) 347
Bpu14I TTCGAA 1 cut(s) 504
BpuEI CTTGAG 1 cut(s) 407
BpuMI CCSGG 1 cut(s) 143
BsaHI GRCGYC 1 cut(s) 8
BsaWI WCCGGW 1 cut(s) 62
Bsc4I CCNNNNNNNGG 2 cut(s) 148, 361
Bse1I ACTGG 1 cut(s) 89
BseGI GGATG 2 cut(s) 256, 477
BseLI CCNNNNNNNGG 2 cut(s) 148, 361
BseMII CTCAG 3 cut(s) 215, 231, 338
BseNI ACTGG 1 cut(s) 89
BseRI GAGGAG 1 cut(s) 500
BseXI GCAGC 2 cut(s) 58, 338
BsgI GTGCAG 1 cut(s) 65
Bsh1236I CGCG 1 cut(s) 213
BshFI GGCC 1 cut(s) 345
BsiSI CCGG 2 cut(s) 63, 142
BslFI GGGAC 1 cut(s) 222
BslI CCNNNNNNNGG 2 cut(s) 148, 361
BsmFI GGGAC 1 cut(s) 222
BsnI GGCC 1 cut(s) 345
Bsp119I TTCGAA 1 cut(s) 504
Bsp143I GATC 1 cut(s) 271
BspACI CCGC 1 cut(s) 338
BspANI GGCC 1 cut(s) 345
BspCNI CTCAG 3 cut(s) 214, 232, 339
BspFNI CGCG 1 cut(s) 213
BspMI ACCTGC 1 cut(s) 31
BspPI GGATC 1 cut(s) 266
BspT104I TTCGAA 1 cut(s) 504
BsrI ACTGG 1 cut(s) 89
BssMI GATC 1 cut(s) 271
BssNI GRCGYC 1 cut(s) 8
Bst6I CTCTTC 1 cut(s) 150
BstACI GRCGYC 1 cut(s) 8
BstBI TTCGAA 1 cut(s) 504
BstDEI CTNAG 3 cut(s) 201, 240, 347
BstF5I GGATG 2 cut(s) 256, 477
BstFNI CGCG 1 cut(s) 213
BstH2I RGCGCY 1 cut(s) 266
BstHHI GCGC 1 cut(s) 265
BstKTI GATC 1 cut(s) 274
BstMBI GATC 1 cut(s) 271
BstMWI GCNNNNNNNGC 5 cut(s) 46, 262, 351, 389, 487
BstSCI CCNGG 1 cut(s) 141
BstUI CGCG 1 cut(s) 213
BstV1I GCAGC 2 cut(s) 58, 338
BstX2I RGATCY 1 cut(s) 271
BstXI CCANNNNNNTGG 1 cut(s) 439
BstYI RGATCY 1 cut(s) 271
BsuRI GGCC 1 cut(s) 345
BtsCI GGATG 2 cut(s) 256, 477
BveI ACCTGC 1 cut(s) 31
CaiI CAGNNNCTG 1 cut(s) 95
CfoI GCGC 1 cut(s) 265
CseI GACGC 2 cut(s) 16, 219
DdeI CTNAG 3 cut(s) 201, 240, 347
DpnI GATC 1 cut(s) 273
DpnII GATC 1 cut(s) 271
DraI TTTAAA 1 cut(s) 396
Eam1104I CTCTTC 1 cut(s) 150
EarI CTCTTC 1 cut(s) 150
Eco147I AGGCCT 1 cut(s) 345
Eco47III AGCGCT 1 cut(s) 264
FaqI GGGAC 1 cut(s) 222
Fnu4HI GCNGC 3 cut(s) 47, 338, 352
FokI GGATG 2 cut(s) 263, 484
Fsp4HI GCNGC 3 cut(s) 47, 338, 352
FspBI CTAG 2 cut(s) 341, 425
GlaI GCGC 1 cut(s) 264
GluI GCNGC 3 cut(s) 47, 338, 352
GsuI CTGGAG 1 cut(s) 503
HaeII RGCGCY 1 cut(s) 266
HaeIII GGCC 1 cut(s) 345
HapII CCGG 2 cut(s) 63, 142
HgaI GACGC 2 cut(s) 16, 219
HhaI GCGC 1 cut(s) 265
Hin1I GRCGYC 1 cut(s) 8
Hin6I GCGC 1 cut(s) 263
HinP1I GCGC 1 cut(s) 263
HinfI GANTC 2 cut(s) 85, 244
HpaII CCGG 2 cut(s) 63, 142
HphI GGTGA 2 cut(s) 193, 464
Hpy188I TCNGA 2 cut(s) 178, 241
Hpy188III TCNNGA 2 cut(s) 248, 386
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 1 cut(s) 355
HpyCH4IV ACGT 1 cut(s) 315
HpyCH4V TGCA 4 cut(s) 40, 46, 128, 293
HpyF10VI GCNNNNNNNGC 5 cut(s) 46, 262, 351, 389, 487
HpyF3I CTNAG 3 cut(s) 201, 240, 347
HpySE526I ACGT 1 cut(s) 315
Hsp92I GRCGYC 1 cut(s) 8
HspAI GCGC 1 cut(s) 263
Kzo9I GATC 1 cut(s) 271
LmnI GCTCC 2 cut(s) 442, 487
LpnPI CCDG 8 cut(s) 26, 76, 102, 155, 182, 233, 359, 467
Lsp1109I GCAGC 2 cut(s) 58, 338
MaeI CTAG 2 cut(s) 341, 425
MaeII ACGT 1 cut(s) 315
MaeIII GTNAC 1 cut(s) 74
MalI GATC 1 cut(s) 273
MboI GATC 1 cut(s) 271
MboII GAAGA 8 cut(s) 67, 119, 122, 167, 178, 181, 230, 487
MfeI CAATTG 1 cut(s) 35
MflI RGATCY 1 cut(s) 271
MluCI AATT 3 cut(s) 35, 294, 434
MluI ACGCGT 1 cut(s) 211
MlyI GAGTC 1 cut(s) 94
MmeI TCCRAC 1 cut(s) 156
MnlI CCTC 6 cut(s) 145, 172, 300, 382, 462, 478
MseI TTAA 3 cut(s) 332, 395, 517
MspI CCGG 2 cut(s) 63, 142
MspR9I CCNGG 1 cut(s) 143
MunI CAATTG 1 cut(s) 35
MvnI CGCG 1 cut(s) 213
MwoI GCNNNNNNNGC 5 cut(s) 46, 262, 351, 389, 487
NciI CCSGG 1 cut(s) 143
NdeII GATC 1 cut(s) 271
NspV TTCGAA 1 cut(s) 504
PaqCI CACCTGC 1 cut(s) 31
PceI AGGCCT 1 cut(s) 345
PfeI GAWTC 1 cut(s) 244
PkrI GCNGC 3 cut(s) 48, 339, 353
PleI GAGTC 1 cut(s) 93
PpsI GAGTC 1 cut(s) 93
PstNI CAGNNNCTG 1 cut(s) 95
PsuI RGATCY 1 cut(s) 271
SaqAI TTAA 3 cut(s) 332, 395, 517
SatI GCNGC 3 cut(s) 47, 338, 352
Sau3AI GATC 1 cut(s) 271
SchI GAGTC 1 cut(s) 94
ScrFI CCNGG 1 cut(s) 143
SfuI TTCGAA 1 cut(s) 504
SmlI CTYRAG 1 cut(s) 386
SmoI CTYRAG 1 cut(s) 386
SpeI ACTAGT 1 cut(s) 424
Sse9I AATT 3 cut(s) 35, 294, 434
SseBI AGGCCT 1 cut(s) 345
SsiI CCGC 1 cut(s) 338
SspMI CTAG 2 cut(s) 341, 425
StuI AGGCCT 1 cut(s) 345
StyD4I CCNGG 1 cut(s) 141
TaiI ACGT 1 cut(s) 318
TaqI TCGA 1 cut(s) 504
TasI AATT 3 cut(s) 35, 294, 434
TauI GCSGC 1 cut(s) 340
TfiI GAWTC 1 cut(s) 244
Tru1I TTAA 3 cut(s) 332, 395, 517
Tru9I TTAA 3 cut(s) 332, 395, 517
TseI GCWGC 2 cut(s) 46, 351
TspDTI ATGAA 1 cut(s) 488
XspI CTAG 2 cut(s) 341, 425
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.