MD03G1147900.v1.1

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0015196
Physical Location & Seq
Forward (+)
16353084 .. 16354911
1828 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1147900.v1.1.491

Sequence Viewer

Length: 1011 bp
ATGAAAATGTCTAGCACAGCTGCAGTGGCACTAGCAATTGGGCTCGTACTCGGGGCATTCTCAGGCCACTGTTCCAGTGCACTGGAGGTAGGATTTTACCGGGGGAAATGTGGCCGGGCTATAGATGTTGAAGAAATTGTAGCGCGTGTCGTAAAGGAAAAGTTTTTCAAAAATCGGACAATTGTTGCTGCCCTCATTCGCATGCAATTTCATGATTGCTTTGTCAATGGATGTGATGCATCCATTCTACTAGATGGAAGCTCCACTGAGAAAACAGCTCCTCCAAACTTAAGTGTACGGGGTTATGATGTCATTGATGCCGCCAAGACCGCCGTGGAAAGCGAATGCCGAGGAGTTGTCTCTTGCGCTGATATTATTGCAATTGCTACCAGAGAAGCTGTATTTTGGGGCGGAGGAGGGCGATACGAAGTACAAACAGGGAGAAGGGATGGCTTCGTATCTCAAGCATCAGATGTTGACCTCCCAAATCCCTCAATGTCAGTGTCTGATTCAGTTGCAGCATTTGCTAGAAAAGGACTCAATGCGAGTGACATGGTTCATCTTCTGGGTGGTCACACTGTTGGGGTGGCACATTGTAACCTCTTCCAAGATCGTCTTTACAATTTCAAAAGCACCGGAAAACCTGACCCCGACATGGAATCTTCATTATTGGAAAGGCTGAGACTTATATGCCCTGAAAACTCAGAAGGCACCAATGCTACTAATCTGGACCAGAACCCTCAAAGTTCATTTATTGTTGATAACTCCTTCTACAAGGAGATAGAAGCCCACAGAGGAGTTCTTCAAATCGACCAAGAACTAGCTGGGGACCCCATAACTCAGGCTATGGTGAAAACCTTAGCCAGCGATACAAATAGCTTCTCCACGAAATTTGGGCAGGCCATGGTTAAGTTGGGAGCAGTTGGAGTCCTTACTACTGAAAAACAAGGAGAGATTAGGAGATCATGCCGGGAAATTAATAACCTTGGCGGTTTTTTAAATTTTAATTGA

Protein Analysis

337

Amino Acids

36.16

Weight (kDa)

5.75

Isoelectric Point (pI)

33.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 47 - 290 1.1e-80 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015196)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G22410
fragaria_vesca FvH4_3g25270
malus_domestica MD03G1147900.v1.1
prunus_persica Prupe.6G132800_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0046861
rosa_laevigata RLG00000034451
rosa_multiflora Rmu_sc0002264.1_g000029
rosa_roxburghii Rroxscaffold_1G00034520
rosa_rugosa Rorug05G0232300
rosa_samantha Rh1BG405200 Rh1DG435700 Rh5AG311600 Rh5BG320600 Rh5CG346700 Rh5DG331300
rosa_wichuraiana Rw5G029120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 710
AccII CGCG 1 cut(s) 145
AciI CCGC 4 cut(s) 321, 330, 411, 990
AcoI YGGCCR 1 cut(s) 112
AcsI RAATTY 2 cut(s) 890, 1000
AfaI GTAC 3 cut(s) 48, 297, 432
AfiI CCNNNNNNNGG 1 cut(s) 655
AflII CTTAAG 1 cut(s) 289
AgsI TTSAA 4 cut(s) 131, 169, 628, 806
AluBI AGCT 6 cut(s) 20, 261, 278, 398, 824, 879
AluI AGCT 6 cut(s) 20, 261, 278, 398, 824, 879
Alw21I GWGCWC 1 cut(s) 82
Alw26I GTCTC 2 cut(s) 364, 676
Alw44I GTGCAC 1 cut(s) 78
AlwNI CAGNNNCTG 1 cut(s) 506
Ama87I CYCGRG 1 cut(s) 50
AoxI GGCC 3 cut(s) 64, 112, 900
ApaLI GTGCAC 1 cut(s) 78
ApeKI GCWGC 3 cut(s) 20, 188, 518
ApoI RAATTY 2 cut(s) 890, 1000
AseI ATTAAT 1 cut(s) 978
AspLEI GCGC 2 cut(s) 145, 368
AspS9I GGNCC 2 cut(s) 730, 829
AsuC2I CCSGG 3 cut(s) 101, 116, 971
AsuHPI GGTGA 1 cut(s) 862
AvaI CYCGRG 1 cut(s) 50
AvaII GGWCC 2 cut(s) 730, 829
BaeGI GKGCMC 1 cut(s) 82
BanI GGYRCC 1 cut(s) 710
BanII GRGCYC 1 cut(s) 45
Bbv12I GWGCWC 1 cut(s) 82
BbvI GCAGC 3 cut(s) 7, 175, 530
BccI CCATC 2 cut(s) 248, 443
BceAI ACGGC 1 cut(s) 317
BcnI CCSGG 3 cut(s) 101, 116, 971
BcoDI GTCTC 2 cut(s) 364, 676
BfaI CTAG 5 cut(s) 12, 32, 251, 528, 821
BfmI CTRYAG 2 cut(s) 21, 120
BfrI CTTAAG 1 cut(s) 289
BisI GCNGC 4 cut(s) 21, 189, 321, 519
BlsI GCNGC 4 cut(s) 22, 190, 322, 520
Bme1390I CCNGG 3 cut(s) 101, 116, 971
Bme18I GGWCC 2 cut(s) 730, 829
BmeT110I CYCGRG 1 cut(s) 50
BmgT120I GGNCC 2 cut(s) 730, 829
BmiI GGNNCC 3 cut(s) 712, 830, 831
BmrFI CCNGG 3 cut(s) 101, 116, 971
BmsI GCATC 4 cut(s) 226, 248, 307, 476
BpmI CTGGAG 1 cut(s) 104
Bpu10I CCTNAGC 1 cut(s) 859
BpuEI CTTGAG 1 cut(s) 447
BpuMI CCSGG 3 cut(s) 101, 116, 971
BsaJI CCNNGG 5 cut(s) 100, 333, 349, 903, 985
BsaWI WCCGGW 1 cut(s) 635
BsaXI ACNNNNNCTCC 2 cut(s) 265, 295
Bsc4I CCNNNNNNNGG 1 cut(s) 655
Bse1I ACTGG 2 cut(s) 75, 87
BseDI CCNNGG 5 cut(s) 100, 333, 349, 903, 985
BseGI GGATG 3 cut(s) 236, 239, 454
BseLI CCNNNNNNNGG 1 cut(s) 655
BseMII CTCAG 5 cut(s) 75, 258, 671, 717, 854
BseNI ACTGG 2 cut(s) 75, 87
BseRI GAGGAG 4 cut(s) 270, 366, 429, 810
BseSI GKGCMC 1 cut(s) 82
BseXI GCAGC 3 cut(s) 7, 175, 530
BseYI CCCAGC 1 cut(s) 824
Bsh1236I CGCG 1 cut(s) 145
BshFI GGCC 3 cut(s) 66, 114, 902
BshNI GGYRCC 1 cut(s) 710
BsiHKAI GWGCWC 1 cut(s) 82
BsiHKCI CYCGRG 1 cut(s) 50
BsiSI CCGG 4 cut(s) 100, 115, 636, 970
BslFI GGGAC 1 cut(s) 842
BslI CCNNNNNNNGG 1 cut(s) 655
BsmAI GTCTC 2 cut(s) 364, 676
BsmFI GGGAC 1 cut(s) 842
BsmI GAATGC 2 cut(s) 56, 350
BsnI GGCC 3 cut(s) 66, 114, 902
BsoBI CYCGRG 1 cut(s) 50
Bsp1286I GDGCHC 2 cut(s) 45, 82
Bsp143I GATC 2 cut(s) 610, 962
Bsp19I CCATGG 1 cut(s) 903
BspACI CCGC 4 cut(s) 321, 330, 411, 990
BspANI GGCC 3 cut(s) 66, 114, 902
BspCNI CTCAG 5 cut(s) 74, 259, 672, 716, 853
BspFNI CGCG 1 cut(s) 145
BspHI TCATGA 1 cut(s) 211
BspLI GGNNCC 3 cut(s) 712, 830, 831
BspMAI CTGCAG 1 cut(s) 25
BspT107I GGYRCC 1 cut(s) 710
BspTI CTTAAG 1 cut(s) 289
BsrI ACTGG 2 cut(s) 75, 87
BssECI CCNNGG 5 cut(s) 100, 333, 349, 903, 985
BssMI GATC 2 cut(s) 610, 962
BssT1I CCWWGG 2 cut(s) 903, 985
Bst4CI ACNGT 2 cut(s) 71, 580
Bst6I CTCTTC 1 cut(s) 608
BstAFI CTTAAG 1 cut(s) 289
BstAPI GCANNNNNTGC 1 cut(s) 524
BstC8I GCNNGC 3 cut(s) 203, 865, 900
BstDEI CTNAG 6 cut(s) 61, 267, 680, 703, 840, 859
BstDSI CCRYGG 2 cut(s) 333, 903
BstF5I GGATG 3 cut(s) 236, 239, 454
BstFNI CGCG 1 cut(s) 145
BstHHI GCGC 2 cut(s) 145, 368
BstKTI GATC 2 cut(s) 613, 965
BstMAI GTCTC 2 cut(s) 364, 676
BstMBI GATC 2 cut(s) 610, 962
BstMWI GCNNNNNNNGC 3 cut(s) 26, 329, 524
BstNSI RCATGY 1 cut(s) 205
BstSCI CCNGG 3 cut(s) 99, 114, 969
BstSFI CTRYAG 2 cut(s) 21, 120
BstSLI GKGCMC 1 cut(s) 82
BstUI CGCG 1 cut(s) 145
BstV1I GCAGC 3 cut(s) 7, 175, 530
BstXI CCANNNNNNTGG 1 cut(s) 82
BsuRI GGCC 3 cut(s) 66, 114, 902
BtgI CCRYGG 2 cut(s) 333, 903
BtsCI GGATG 3 cut(s) 236, 239, 454
BtsI GCAGTG 1 cut(s) 30
BtsIMutI CAGTG 7 cut(s) 30, 67, 80, 82, 264, 507, 576
Cac8I GCNNGC 3 cut(s) 203, 865, 900
CaiI CAGNNNCTG 1 cut(s) 506
CciI TCATGA 1 cut(s) 211
CfoI GCGC 2 cut(s) 145, 368
Cfr13I GGNCC 2 cut(s) 730, 829
Csp6I GTAC 3 cut(s) 47, 296, 431
CspCI CAANNNNNGTGG 2 cut(s) 874, 909
CviAII CATG 6 cut(s) 202, 212, 553, 655, 904, 966
CviQI GTAC 3 cut(s) 47, 296, 431
DdeI CTNAG 6 cut(s) 61, 267, 680, 703, 840, 859
DpnI GATC 2 cut(s) 612, 964
DpnII GATC 2 cut(s) 610, 962
DraI TTTAAA 1 cut(s) 999
EaeI YGGCCR 1 cut(s) 112
Eam1104I CTCTTC 1 cut(s) 608
EarI CTCTTC 1 cut(s) 608
EciI GGCGGA 1 cut(s) 426
Eco130I CCWWGG 2 cut(s) 903, 985
Eco24I GRGCYC 1 cut(s) 45
Eco47I GGWCC 2 cut(s) 730, 829
Eco88I CYCGRG 1 cut(s) 50
EcoO109I RGGNCCY 1 cut(s) 829
EcoT14I CCWWGG 2 cut(s) 903, 985
EcoT22I ATGCAT 1 cut(s) 241
EcoT38I GRGCYC 1 cut(s) 45
ErhI CCWWGG 2 cut(s) 903, 985
FaeI CATG 6 cut(s) 205, 215, 556, 658, 907, 969
FalI AAGNNNNNCTT 2 cut(s) 600, 632
FaqI GGGAC 1 cut(s) 842
FatI CATG 6 cut(s) 201, 211, 552, 654, 903, 965
Fnu4HI GCNGC 4 cut(s) 21, 189, 321, 519
FokI GGATG 3 cut(s) 226, 243, 461
FriOI GRGCYC 1 cut(s) 45
Fsp4HI GCNGC 4 cut(s) 21, 189, 321, 519
FspBI CTAG 5 cut(s) 12, 32, 251, 528, 821
GlaI GCGC 2 cut(s) 144, 367
GluI GCNGC 4 cut(s) 21, 189, 321, 519
GsaI CCCAGC 1 cut(s) 828
GsuI CTGGAG 1 cut(s) 104
HaeIII GGCC 3 cut(s) 66, 114, 902
HapII CCGG 4 cut(s) 100, 115, 636, 970
HhaI GCGC 2 cut(s) 145, 368
Hin1II CATG 6 cut(s) 205, 215, 556, 658, 907, 969
Hin6I GCGC 2 cut(s) 143, 366
HinP1I GCGC 2 cut(s) 143, 366
HincII GTYRAC 1 cut(s) 478
HindII GTYRAC 1 cut(s) 478
HinfI GANTC 4 cut(s) 509, 537, 659, 927
HpaII CCGG 4 cut(s) 100, 115, 636, 970
HphI GGTGA 1 cut(s) 862
Hpy166II GTNNAC 3 cut(s) 80, 296, 478
Hpy188I TCNGA 4 cut(s) 177, 472, 508, 706
Hpy188III TCNNGA 2 cut(s) 212, 728
Hpy8I GTNNAC 3 cut(s) 80, 296, 478
HpyAV CCTTC 3 cut(s) 438, 701, 778
HpyCH4III ACNGT 2 cut(s) 71, 580
HpyCH4V TGCA 6 cut(s) 23, 80, 205, 239, 380, 518
HpyF10VI GCNNNNNNNGC 3 cut(s) 26, 329, 524
HpyF3I CTNAG 6 cut(s) 61, 267, 680, 703, 840, 859
Hsp92II CATG 6 cut(s) 205, 215, 556, 658, 907, 969
HspAI GCGC 2 cut(s) 143, 366
KflI GGGWCCC 1 cut(s) 829
Kzo9I GATC 2 cut(s) 610, 962
LmnI GCTCC 3 cut(s) 266, 283, 917
Lsp1109I GCAGC 3 cut(s) 7, 175, 530
LweI GCATC 4 cut(s) 226, 248, 307, 476
MaeI CTAG 5 cut(s) 12, 32, 251, 528, 821
MaeIII GTNAC 3 cut(s) 548, 572, 596
MalI GATC 2 cut(s) 612, 964
MboI GATC 2 cut(s) 610, 962
MboII GAAGA 5 cut(s) 143, 554, 595, 654, 794
MfeI CAATTG 3 cut(s) 36, 180, 381
MhlI GDGCHC 2 cut(s) 45, 82
MlyI GAGTC 2 cut(s) 531, 936
MmeI TCCRAC 1 cut(s) 904
Mph1103I ATGCAT 1 cut(s) 241
MseI TTAA 5 cut(s) 290, 909, 978, 998, 1005
MslI CAYNNNNRTG 1 cut(s) 200
MspA1I CMGCKG 1 cut(s) 20
MspCI CTTAAG 1 cut(s) 289
MspI CCGG 4 cut(s) 100, 115, 636, 970
MspR9I CCNGG 3 cut(s) 101, 116, 971
MunI CAATTG 3 cut(s) 36, 180, 381
Mva1269I GAATGC 2 cut(s) 56, 350
MvnI CGCG 1 cut(s) 145
MwoI GCNNNNNNNGC 3 cut(s) 26, 329, 524
NciI CCSGG 3 cut(s) 101, 116, 971
NcoI CCATGG 1 cut(s) 903
NdeII GATC 2 cut(s) 610, 962
NlaIII CATG 6 cut(s) 205, 215, 556, 658, 907, 969
NlaIV GGNNCC 3 cut(s) 712, 830, 831
NmeAIII GCCGAG 1 cut(s) 374
NmuCI GTSAC 2 cut(s) 548, 572
NsiI ATGCAT 1 cut(s) 241
NspI RCATGY 1 cut(s) 205
PaeI GCATGC 1 cut(s) 205
PagI TCATGA 1 cut(s) 211
PctI GAATGC 2 cut(s) 56, 350
PfeI GAWTC 2 cut(s) 509, 659
PkrI GCNGC 4 cut(s) 22, 190, 322, 520
PleI GAGTC 2 cut(s) 531, 935
PpsI GAGTC 2 cut(s) 531, 935
PpuMI RGGWCCY 1 cut(s) 829
PshBI ATTAAT 1 cut(s) 978
Psp5II RGGWCCY 1 cut(s) 829
PspFI CCCAGC 1 cut(s) 824
PspN4I GGNNCC 3 cut(s) 712, 830, 831
PspPI GGNCC 2 cut(s) 730, 829
PspPPI RGGWCCY 1 cut(s) 829
PstI CTGCAG 1 cut(s) 25
PstNI CAGNNNCTG 1 cut(s) 506
PvuII CAGCTG 1 cut(s) 20
RsaI GTAC 3 cut(s) 48, 297, 432
RsaNI GTAC 3 cut(s) 47, 296, 431
RseI CAYNNNNRTG 1 cut(s) 200
SaqAI TTAA 5 cut(s) 290, 909, 978, 998, 1005
SatI GCNGC 4 cut(s) 21, 189, 321, 519
Sau3AI GATC 2 cut(s) 610, 962
Sau96I GGNCC 2 cut(s) 730, 829
SchI GAGTC 2 cut(s) 531, 936
ScrFI CCNGG 3 cut(s) 101, 116, 971
SduI GDGCHC 2 cut(s) 45, 82
SfaNI GCATC 4 cut(s) 226, 248, 307, 476
SfcI CTRYAG 2 cut(s) 21, 120
SinI GGWCC 2 cut(s) 730, 829
SmiMI CAYNNNNRTG 1 cut(s) 200
SmlI CTYRAG 2 cut(s) 289, 462
SmoI CTYRAG 2 cut(s) 289, 462
SphI GCATGC 1 cut(s) 205
SsiI CCGC 4 cut(s) 321, 330, 411, 990
SspMI CTAG 5 cut(s) 12, 32, 251, 528, 821
StyD4I CCNGG 3 cut(s) 99, 114, 969
StyI CCWWGG 2 cut(s) 903, 985
TaaI ACNGT 2 cut(s) 71, 580
TaqI TCGA 1 cut(s) 810
TatI WGTACW 1 cut(s) 430
TauI GCSGC 1 cut(s) 323
TfiI GAWTC 2 cut(s) 509, 659
Tru1I TTAA 5 cut(s) 290, 909, 978, 998, 1005
Tru9I TTAA 5 cut(s) 290, 909, 978, 998, 1005
TscAI CASTG 7 cut(s) 30, 74, 82, 87, 271, 507, 583
TseFI GTSAC 2 cut(s) 548, 572
TseI GCWGC 3 cut(s) 20, 188, 518
Tsp45I GTSAC 2 cut(s) 548, 572
TspDTI ATGAA 5 cut(s) 17, 200, 548, 654, 738
TspRI CASTG 7 cut(s) 30, 74, 82, 87, 271, 507, 583
Vha464I CTTAAG 1 cut(s) 289
VneI GTGCAC 1 cut(s) 78
VpaK11BI GGWCC 2 cut(s) 730, 829
VspI ATTAAT 1 cut(s) 978
XapI RAATTY 2 cut(s) 890, 1000
XceI RCATGY 1 cut(s) 205
XcmI CCANNNNNNNNNTGG 3 cut(s) 331, 821, 910
XspI CTAG 5 cut(s) 12, 32, 251, 528, 821
Zsp2I ATGCAT 1 cut(s) 241
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.