Rw5G029120

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0015196
Physical Location & Seq
Reverse (-)
44364239 .. 44367162
2924 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G029120.1

Sequence Viewer

Length: 987 bp
ATGAAAATGTCTAGCAGAGCTCTAGTGGCAGTAGGGATATTTGGGCTCATATTTGTAAGCCTTACAGGGCAGTGCTATGGGGCACTAGAGTTAGGGTTCTACGAGAGAAAGTGCCTTGCTGATGTTGAAAAAGTAGTAGCTGATGTAGTCATGGCAAAGTACAAAATGGACCGAACAATTGCTGCTGCTCTCGTTCGCATGCAGTTCCACGATTGCTTTGTGAATGGATGTGATGCATCCATTCTACTAGATGGAAGCTCCAGTGAGAAAACAGCTCCTCCGAATCTCAGTGTACGGGGTTATGATGTCATTGATGCCGCAAAAGCTGCAGTTGAAGTTGTATGCCCAGGAAAGGTCTCCTGCTCGGATATCATTGTAATGGCTACCAGAGAAGCTGTATGTCTGAGTGGAGGAGGGCGATACAGTGTTCAAACTGGGAGAAGAGATGGCTTTGTATCTCTTGCATCAAATGTTGATCTCCCAGCTCCATCAATTTCAGTGAATCAATCTGTTGCAGCATTTGCTAGAAAAGGACTCAATGCCATTGACATGGTTTATCTTCTTGGCGGTCACACCATTGGGGTGGCACATTGTTTTCTCTTCCAAGACCGTCTATACAATTTCCAAAACACTGGCGGACCTGACCGGAGCATGGATTCATCATTGCTGCGAAAGCTGAGATCTATCTGCCCTCAAAATTCAGCAGGCAGTAAAACTGCTGATCTTGACCAGAACGAGTACGTACCAAGTTCGTTCATTGTTGACAACTCATTCTACAAGCAAATACGAGCCAGGAGAGGAGTTCTTCAAATCGACCAAGAGCTAGCTTTCGACCCCATAACTCAGTCCACAGTGACAACAATTGCCAATAGTTTTGGCTTCTCCGTGAAATTTGGGCAGGCCATGGTTAAGTTGGGAGCAGTGGAAGTACTTACAGGCACGCAAGGGGAGATCAGGAAATCATGCAGAGCAGTTAACACTCCTTGA

Protein Analysis

328

Amino Acids

35.26

Weight (kDa)

8.48

Isoelectric Point (pI)

34.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 46 - 291 3.4e-78 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015196)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G22410
fragaria_vesca FvH4_3g25270
malus_domestica MD03G1147900.v1.1
prunus_persica Prupe.6G132800_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0046861
rosa_laevigata RLG00000034451
rosa_multiflora Rmu_sc0002264.1_g000029
rosa_roxburghii Rroxscaffold_1G00034520
rosa_rugosa Rorug05G0232300
rosa_samantha Rh1BG405200 Rh1DG435700 Rh5AG311600 Rh5BG320600 Rh5CG346700 Rh5DG331300
rosa_wichuraiana Rw5G029120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 318, 567, 636
AcsI RAATTY 2 cut(s) 697, 890
AfaI GTAC 5 cut(s) 161, 294, 740, 744, 930
AfiI CCNNNNNNNGG 2 cut(s) 352, 652
AgsI TTSAA 4 cut(s) 128, 335, 431, 809
AjnI CCWGG 2 cut(s) 346, 791
Alw21I GWGCWC 1 cut(s) 22
Alw26I GTCTC 1 cut(s) 361
AoxI GGCC 1 cut(s) 900
ApeKI GCWGC 5 cut(s) 182, 185, 326, 515, 667
ApoI RAATTY 2 cut(s) 697, 890
AspS9I GGNCC 2 cut(s) 169, 638
AsuNHI GCTAGC 1 cut(s) 823
AvaII GGWCC 2 cut(s) 169, 638
BaeGI GKGCMC 1 cut(s) 85
BanII GRGCYC 2 cut(s) 22, 48
Bbv12I GWGCWC 1 cut(s) 22
BbvI GCAGC 5 cut(s) 169, 172, 313, 527, 654
BccI CCATC 3 cut(s) 245, 440, 496
BciT130I CCWGG 2 cut(s) 348, 793
BcoDI GTCTC 1 cut(s) 361
BfaI CTAG 6 cut(s) 12, 23, 86, 248, 525, 824
BfmI CTRYAG 1 cut(s) 327
BglII AGATCT 1 cut(s) 680
BisI GCNGC 6 cut(s) 183, 186, 318, 327, 516, 668
BlsI GCNGC 6 cut(s) 184, 187, 319, 328, 517, 669
BmcAI AGTACT 1 cut(s) 930
Bme1390I CCNGG 2 cut(s) 348, 793
Bme18I GGWCC 2 cut(s) 169, 638
BmgT120I GGNCC 2 cut(s) 169, 638
BmrFI CCNGG 2 cut(s) 348, 793
BmrI ACTGGG 1 cut(s) 444
BmsI GCATC 4 cut(s) 223, 245, 304, 473
BmtI GCTAGC 1 cut(s) 827
BmuI ACTGGG 1 cut(s) 444
BpmI CTGGAG 1 cut(s) 244
BsaAI YACGTR 1 cut(s) 742
BsaI GGTCTC 1 cut(s) 361
BsaJI CCNNGG 2 cut(s) 346, 903
BsaWI WCCGGW 1 cut(s) 645
BsaXI ACNNNNNCTCC 2 cut(s) 262, 292
Bsc4I CCNNNNNNNGG 2 cut(s) 352, 652
Bse1I ACTGG 3 cut(s) 261, 439, 637
Bse3DI GCAATG 1 cut(s) 662
BseBI CCWGG 2 cut(s) 348, 793
BseDI CCNNGG 2 cut(s) 346, 903
BseGI GGATG 2 cut(s) 233, 236
BseLI CCNNNNNNNGG 2 cut(s) 352, 652
BseMI GCAATG 1 cut(s) 662
BseMII CTCAG 4 cut(s) 301, 395, 668, 857
BseNI ACTGG 3 cut(s) 261, 439, 637
BseRI GAGGAG 3 cut(s) 267, 426, 813
BseSI GKGCMC 1 cut(s) 85
BseXI GCAGC 5 cut(s) 169, 172, 313, 527, 654
BseYI CCCAGC 1 cut(s) 481
BshFI GGCC 1 cut(s) 902
BsiHKAI GWGCWC 1 cut(s) 22
BsiSI CCGG 1 cut(s) 646
BslI CCNNNNNNNGG 2 cut(s) 352, 652
BsmAI GTCTC 1 cut(s) 361
BsnI GGCC 1 cut(s) 902
Bso31I GGTCTC 1 cut(s) 361
Bsp1286I GDGCHC 3 cut(s) 22, 48, 85
Bsp143I GATC 4 cut(s) 475, 680, 721, 951
Bsp19I CCATGG 1 cut(s) 903
BspACI CCGC 3 cut(s) 318, 567, 636
BspANI GGCC 1 cut(s) 902
BspCNI CTCAG 4 cut(s) 300, 396, 669, 856
BspMAI CTGCAG 1 cut(s) 331
BspOI GCTAGC 1 cut(s) 827
BspTNI GGTCTC 1 cut(s) 361
BsrDI GCAATG 1 cut(s) 662
BsrI ACTGG 3 cut(s) 261, 439, 637
BssECI CCNNGG 2 cut(s) 346, 903
BssMI GATC 4 cut(s) 475, 680, 721, 951
BssT1I CCWWGG 1 cut(s) 903
Bst2UI CCWGG 2 cut(s) 348, 793
Bst4CI ACNGT 3 cut(s) 425, 611, 853
Bst6I CTCTTC 2 cut(s) 436, 605
BstAPI GCANNNNNTGC 2 cut(s) 326, 521
BstBAI YACGTR 1 cut(s) 742
BstC8I GCNNGC 5 cut(s) 200, 706, 825, 900, 941
BstDEI CTNAG 4 cut(s) 287, 404, 677, 843
BstDSI CCRYGG 1 cut(s) 903
BstF5I GGATG 2 cut(s) 233, 236
BstKTI GATC 4 cut(s) 478, 683, 724, 954
BstMAI GTCTC 1 cut(s) 361
BstMBI GATC 4 cut(s) 475, 680, 721, 951
BstMWI GCNNNNNNNGC 5 cut(s) 26, 323, 326, 521, 673
BstNI CCWGG 2 cut(s) 348, 793
BstNSI RCATGY 1 cut(s) 202
BstSCI CCNGG 2 cut(s) 346, 791
BstSFI CTRYAG 1 cut(s) 327
BstSLI GKGCMC 1 cut(s) 85
BstSNI TACGTA 1 cut(s) 742
BstV1I GCAGC 5 cut(s) 169, 172, 313, 527, 654
BstX2I RGATCY 1 cut(s) 680
BstXI CCANNNNNNTGG 3 cut(s) 550, 583, 632
BstYI RGATCY 1 cut(s) 680
BsuRI GGCC 1 cut(s) 902
BtgI CCRYGG 1 cut(s) 903
BtsCI GGATG 2 cut(s) 233, 236
BtsI GCAGTG 2 cut(s) 77, 927
BtsIMutI CAGTG 8 cut(s) 77, 268, 295, 430, 504, 630, 858, 927
Cac8I GCNNGC 5 cut(s) 200, 706, 825, 900, 941
Cfr13I GGNCC 2 cut(s) 169, 638
Csp6I GTAC 5 cut(s) 160, 293, 739, 743, 929
CviAII CATG 6 cut(s) 151, 199, 550, 652, 904, 963
CviQI GTAC 5 cut(s) 160, 293, 739, 743, 929
DdeI CTNAG 4 cut(s) 287, 404, 677, 843
DpnI GATC 4 cut(s) 477, 682, 723, 953
DpnII GATC 4 cut(s) 475, 680, 721, 951
Eam1104I CTCTTC 2 cut(s) 436, 605
EarI CTCTTC 2 cut(s) 436, 605
EciI GGCGGA 1 cut(s) 651
Ecl136II GAGCTC 1 cut(s) 20
Eco105I TACGTA 1 cut(s) 742
Eco130I CCWWGG 1 cut(s) 903
Eco24I GRGCYC 2 cut(s) 22, 48
Eco31I GGTCTC 1 cut(s) 361
Eco32I GATATC 1 cut(s) 370
Eco47I GGWCC 2 cut(s) 169, 638
Eco53kI GAGCTC 1 cut(s) 20
EcoICRI GAGCTC 1 cut(s) 20
EcoRII CCWGG 2 cut(s) 346, 791
EcoRV GATATC 1 cut(s) 370
EcoT14I CCWWGG 1 cut(s) 903
EcoT22I ATGCAT 1 cut(s) 238
EcoT38I GRGCYC 2 cut(s) 22, 48
ErhI CCWWGG 1 cut(s) 903
FaeI CATG 6 cut(s) 154, 202, 553, 655, 907, 966
FatI CATG 6 cut(s) 150, 198, 549, 651, 903, 962
Fnu4HI GCNGC 6 cut(s) 183, 186, 318, 327, 516, 668
FokI GGATG 2 cut(s) 223, 240
FriOI GRGCYC 2 cut(s) 22, 48
Fsp4HI GCNGC 6 cut(s) 183, 186, 318, 327, 516, 668
FspBI CTAG 6 cut(s) 12, 23, 86, 248, 525, 824
GluI GCNGC 6 cut(s) 183, 186, 318, 327, 516, 668
GsaI CCCAGC 1 cut(s) 485
GsuI CTGGAG 1 cut(s) 244
HaeIII GGCC 1 cut(s) 902
HapII CCGG 1 cut(s) 646
Hin1II CATG 6 cut(s) 154, 202, 553, 655, 907, 966
HincII GTYRAC 2 cut(s) 763, 976
HindII GTYRAC 2 cut(s) 763, 976
HinfI GANTC 4 cut(s) 283, 502, 534, 656
HpaI GTTAAC 1 cut(s) 976
HpaII CCGG 1 cut(s) 646
Hpy166II GTNNAC 4 cut(s) 293, 763, 849, 976
Hpy188I TCNGA 3 cut(s) 282, 367, 405
Hpy188III TCNNGA 2 cut(s) 725, 955
Hpy8I GTNNAC 4 cut(s) 293, 763, 849, 976
HpyCH4III ACNGT 3 cut(s) 425, 611, 853
HpyCH4IV ACGT 1 cut(s) 741
HpyCH4V TGCA 6 cut(s) 202, 236, 329, 464, 515, 966
HpyF10VI GCNNNNNNNGC 5 cut(s) 26, 323, 326, 521, 673
HpyF3I CTNAG 4 cut(s) 287, 404, 677, 843
HpySE526I ACGT 1 cut(s) 741
Hsp92II CATG 6 cut(s) 154, 202, 553, 655, 907, 966
KspAI GTTAAC 1 cut(s) 976
Kzo9I GATC 4 cut(s) 475, 680, 721, 951
LmnI GCTCC 5 cut(s) 263, 280, 490, 648, 917
Lsp1109I GCAGC 5 cut(s) 169, 172, 313, 527, 654
LweI GCATC 4 cut(s) 223, 245, 304, 473
MaeI CTAG 6 cut(s) 12, 23, 86, 248, 525, 824
MaeII ACGT 1 cut(s) 741
MaeIII GTNAC 2 cut(s) 569, 853
MalI GATC 4 cut(s) 477, 682, 723, 953
MboI GATC 4 cut(s) 475, 680, 721, 951
MboII GAAGA 4 cut(s) 453, 551, 592, 797
MfeI CAATTG 2 cut(s) 177, 861
MflI RGATCY 1 cut(s) 680
MhlI GDGCHC 3 cut(s) 22, 48, 85
MluCI AATT 6 cut(s) 177, 492, 619, 697, 861, 890
MlyI GAGTC 1 cut(s) 528
MnlI CCTC 5 cut(s) 288, 404, 407, 702, 791
Mph1103I ATGCAT 1 cut(s) 238
MseI TTAA 2 cut(s) 909, 975
MslI CAYNNNNRTG 3 cut(s) 377, 548, 581
MspI CCGG 1 cut(s) 646
MspR9I CCNGG 2 cut(s) 348, 793
MunI CAATTG 2 cut(s) 177, 861
MvaI CCWGG 2 cut(s) 348, 793
MwoI GCNNNNNNNGC 5 cut(s) 26, 323, 326, 521, 673
NcoI CCATGG 1 cut(s) 903
NdeII GATC 4 cut(s) 475, 680, 721, 951
NheI GCTAGC 1 cut(s) 823
NlaIII CATG 6 cut(s) 154, 202, 553, 655, 907, 966
NmuCI GTSAC 2 cut(s) 569, 853
NsiI ATGCAT 1 cut(s) 238
NspI RCATGY 1 cut(s) 202
PaeI GCATGC 1 cut(s) 202
PfeI GAWTC 3 cut(s) 283, 502, 656
PkrI GCNGC 6 cut(s) 184, 187, 319, 328, 517, 669
PleI GAGTC 1 cut(s) 528
PpsI GAGTC 1 cut(s) 528
Ppu21I YACGTR 1 cut(s) 742
Psp124BI GAGCTC 1 cut(s) 22
Psp6I CCWGG 2 cut(s) 346, 791
PspFI CCCAGC 1 cut(s) 481
PspGI CCWGG 2 cut(s) 346, 791
PspPI GGNCC 2 cut(s) 169, 638
PstI CTGCAG 1 cut(s) 331
PsuI RGATCY 1 cut(s) 680
RsaI GTAC 5 cut(s) 161, 294, 740, 744, 930
RsaNI GTAC 5 cut(s) 160, 293, 739, 743, 929
RseI CAYNNNNRTG 3 cut(s) 377, 548, 581
SacI GAGCTC 1 cut(s) 22
SaqAI TTAA 2 cut(s) 909, 975
SatI GCNGC 6 cut(s) 183, 186, 318, 327, 516, 668
Sau3AI GATC 4 cut(s) 475, 680, 721, 951
Sau96I GGNCC 2 cut(s) 169, 638
ScaI AGTACT 1 cut(s) 930
SchI GAGTC 1 cut(s) 528
ScrFI CCNGG 2 cut(s) 348, 793
SduI GDGCHC 3 cut(s) 22, 48, 85
SfaNI GCATC 4 cut(s) 223, 245, 304, 473
SfcI CTRYAG 1 cut(s) 327
SinI GGWCC 2 cut(s) 169, 638
SmiMI CAYNNNNRTG 3 cut(s) 377, 548, 581
SnaBI TACGTA 1 cut(s) 742
SphI GCATGC 1 cut(s) 202
Sse9I AATT 6 cut(s) 177, 492, 619, 697, 861, 890
SsiI CCGC 3 cut(s) 318, 567, 636
SspMI CTAG 6 cut(s) 12, 23, 86, 248, 525, 824
SstI GAGCTC 1 cut(s) 22
StyD4I CCNGG 2 cut(s) 346, 791
StyI CCWWGG 1 cut(s) 903
TaaI ACNGT 3 cut(s) 425, 611, 853
TaiI ACGT 1 cut(s) 744
TaqI TCGA 2 cut(s) 813, 831
TaqII GACCGA 1 cut(s) 186
TasI AATT 6 cut(s) 177, 492, 619, 697, 861, 890
TatI WGTACW 2 cut(s) 159, 928
TauI GCSGC 1 cut(s) 320
TfiI GAWTC 3 cut(s) 283, 502, 656
Tru1I TTAA 2 cut(s) 909, 975
Tru9I TTAA 2 cut(s) 909, 975
TscAI CASTG 8 cut(s) 77, 268, 295, 430, 504, 637, 858, 927
TseFI GTSAC 2 cut(s) 569, 853
TseI GCWGC 5 cut(s) 182, 185, 326, 515, 667
Tsp45I GTSAC 2 cut(s) 569, 853
TspDTI ATGAA 3 cut(s) 17, 648, 745
TspGWI ACGGA 1 cut(s) 874
TspRI CASTG 8 cut(s) 77, 268, 295, 430, 504, 637, 858, 927
VpaK11BI GGWCC 2 cut(s) 169, 638
XapI RAATTY 2 cut(s) 697, 890
XceI RCATGY 1 cut(s) 202
XcmI CCANNNNNNNNNTGG 1 cut(s) 910
XspI CTAG 6 cut(s) 12, 23, 86, 248, 525, 824
ZrmI AGTACT 1 cut(s) 930
Zsp2I ATGCAT 1 cut(s) 238
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.