MD03G1170100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0006259
Physical Location & Seq
Forward (+)
23072230 .. 23075570
3341 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1170100.v1.1.491

Sequence Viewer

Length: 864 bp
ATGTCAACGACGGAAGCTCTCTACTCCAAACTCTACGAAAAGTACACAAAAATCAAGACGAAGAAATGGTCGGAGTTGGATGAAATTGGTAGAGAACAAGAAGGAAAGTTCAAGCATTATGTATCTGTTGCAGAGGAGTATATACAACATTTGCGAAATCAAAATGAGAGGTTGCGCTTGGAAGTTGATGAGCTGAGAAGTGAAGTGGCTTCTATTAGGTCCACCAAAGATGAACAATGTACCGAGTACCAGAATCTTTTGATGGAAGAGAACAGGACAAATAAAGAACTTTCTGAAGAAGTAGAGAGGCTCCGAAAGCTGCAACAGGTGGGAAATCTATCGAATGATAGCAACACAGACAATGGTGGACTGTGTACACCTGCTAGTGGTCAAGTATCCGGAGCAGTAGGAAATCTCTCAAAGAGAAGGACAAGTAAACGTAGGAAGAATTCCCACTCTGAGACAGAGGATATGGCTATGCCTTCTAGTGGGGCGGTTATAACTGTTCAAGAGCCACAATGGTGTAGAACTATTGAAAGACAAGGTTGTGCAAAACCAATTGGCCAAGTGAACTGCGTGTTTCAAGTGCTTGTTGAGTATATGGTGGATTTAAAGTTCTCAACTGTTAGTCAAGCCGAAGAAATATGCATCTCAGCTGTGCATCAATCAAGTGGTTATGCCTTCAATCTTACATGGATGAACAGAGCTGCTGGTAACGAAGTAGAGCTTCTGTACCGCACCTCATCCTTGGGGACCCTTGAGAGGGTAGCACCAGAATGGATGAAGGATGACATAGTGTTAAGCCCAAACATGTGCCCTATCTTCTTTGAAAGGTTATCTCGTGTTGTCAAGCTGCACTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

288

Amino Acids

32.8

Weight (kDa)

6.06

Isoelectric Point (pI)

52.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7806 PF25091 190 - 284 1.5e-43 Domain of unknown function (DUF7806)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 500
AarI CACCTGC 1 cut(s) 388
Acc36I ACCTGC 1 cut(s) 388
AccIII TCCGGA 1 cut(s) 398
AciI CCGC 2 cut(s) 494, 736
AcoI YGGCCR 1 cut(s) 562
AcsI RAATTY 1 cut(s) 448
AcuI CTGAAG 1 cut(s) 315
AfaI GTAC 5 cut(s) 44, 241, 248, 376, 734
AfiI CCNNNNNNNGG 4 cut(s) 386, 488, 762, 763
AflIII ACRYGT 1 cut(s) 810
AgsI TTSAA 6 cut(s) 112, 509, 536, 584, 685, 830
AleI CACNNNNGTG 1 cut(s) 520
AloI GAACNNNNNNTCC 2 cut(s) 599, 631
AluBI AGCT 7 cut(s) 17, 193, 319, 656, 707, 727, 853
AluI AGCT 7 cut(s) 17, 193, 319, 656, 707, 727, 853
Alw26I GTCTC 1 cut(s) 455
Aor13HI TCCGGA 1 cut(s) 398
AoxI GGCC 1 cut(s) 562
ApeKI GCWGC 3 cut(s) 319, 707, 853
ApoI RAATTY 1 cut(s) 448
ArsI GACNNNNNNTTYG 2 cut(s) 53, 85
AspLEI GCGC 1 cut(s) 177
AspS9I GGNCC 2 cut(s) 219, 753
AvaII GGWCC 2 cut(s) 219, 753
BaeGI GKGCMC 1 cut(s) 818
BalI TGGCCA 1 cut(s) 564
BauI CACGAG 1 cut(s) 840
BbvI GCAGC 3 cut(s) 306, 694, 840
BccI CCATC 1 cut(s) 256
BciVI GTATCC 1 cut(s) 406
BcoDI GTCTC 1 cut(s) 455
BfaI CTAG 3 cut(s) 384, 486, 862
BfuAI ACCTGC 1 cut(s) 388
BfuI GTATCC 1 cut(s) 406
BisI GCNGC 3 cut(s) 320, 708, 854
BlsI GCNGC 3 cut(s) 321, 709, 855
Bme18I GGWCC 2 cut(s) 219, 753
BmgT120I GGNCC 2 cut(s) 219, 753
BmiI GGNNCC 3 cut(s) 311, 754, 755
BmsI GCATC 2 cut(s) 657, 670
BpuEI CTTGAG 1 cut(s) 779
BsaJI CCNNGG 1 cut(s) 747
BsaWI WCCGGW 1 cut(s) 398
Bsc4I CCNNNNNNNGG 4 cut(s) 386, 488, 762, 763
BseAI TCCGGA 1 cut(s) 398
BseDI CCNNGG 1 cut(s) 747
BseGI GGATG 5 cut(s) 85, 702, 743, 786, 793
BseLI CCNNNNNNNGG 4 cut(s) 386, 488, 762, 763
BseMII CTCAG 3 cut(s) 185, 450, 666
BseRI GAGGAG 1 cut(s) 149
BseSI GKGCMC 1 cut(s) 818
BseXI GCAGC 3 cut(s) 306, 694, 840
BsgI GTGCAG 1 cut(s) 839
BshFI GGCC 1 cut(s) 564
BsiSI CCGG 1 cut(s) 399
BslFI GGGAC 1 cut(s) 766
BslI CCNNNNNNNGG 4 cut(s) 386, 488, 762, 763
BsmAI GTCTC 1 cut(s) 455
BsmFI GGGAC 1 cut(s) 766
BsnI GGCC 1 cut(s) 564
Bsp1286I GDGCHC 1 cut(s) 818
Bsp13I TCCGGA 1 cut(s) 398
Bsp1407I TGTACA 1 cut(s) 374
BspACI CCGC 2 cut(s) 494, 736
BspANI GGCC 1 cut(s) 564
BspCNI CTCAG 3 cut(s) 186, 451, 665
BspEI TCCGGA 1 cut(s) 398
BspLI GGNNCC 3 cut(s) 311, 754, 755
BspMI ACCTGC 1 cut(s) 388
BsrGI TGTACA 1 cut(s) 374
BssECI CCNNGG 1 cut(s) 747
BssSI CACGAG 1 cut(s) 840
BssT1I CCWWGG 1 cut(s) 747
Bst2BI CACGAG 1 cut(s) 840
Bst4CI ACNGT 3 cut(s) 372, 505, 625
Bst6I CTCTTC 1 cut(s) 261
BstAUI TGTACA 1 cut(s) 374
BstDEI CTNAG 3 cut(s) 194, 459, 652
BstF5I GGATG 5 cut(s) 85, 702, 743, 786, 793
BstHHI GCGC 1 cut(s) 177
BstMAI GTCTC 1 cut(s) 455
BstMWI GCNNNNNNNGC 1 cut(s) 316
BstNSI RCATGY 1 cut(s) 814
BstSLI GKGCMC 1 cut(s) 818
BstV1I GCAGC 3 cut(s) 306, 694, 840
BsuI GTATCC 1 cut(s) 406
BsuRI GGCC 1 cut(s) 564
BtsCI GGATG 5 cut(s) 85, 702, 743, 786, 793
BveI ACCTGC 1 cut(s) 388
CfoI GCGC 1 cut(s) 177
Cfr13I GGNCC 2 cut(s) 219, 753
Csp6I GTAC 5 cut(s) 43, 240, 247, 375, 733
CviAII CATG 2 cut(s) 693, 811
CviQI GTAC 5 cut(s) 43, 240, 247, 375, 733
DdeI CTNAG 3 cut(s) 194, 459, 652
DraI TTTAAA 1 cut(s) 612
EaeI YGGCCR 1 cut(s) 562
Eam1104I CTCTTC 1 cut(s) 261
EarI CTCTTC 1 cut(s) 261
Eco130I CCWWGG 1 cut(s) 747
Eco47I GGWCC 2 cut(s) 219, 753
Eco57I CTGAAG 1 cut(s) 315
EcoO109I RGGNCCY 1 cut(s) 753
EcoRI GAATTC 1 cut(s) 448
EcoT14I CCWWGG 1 cut(s) 747
EcoT22I ATGCAT 1 cut(s) 650
ErhI CCWWGG 1 cut(s) 747
FaeI CATG 2 cut(s) 696, 814
FalI AAGNNNNNCTT 2 cut(s) 711, 743
FaqI GGGAC 1 cut(s) 766
FatI CATG 2 cut(s) 692, 810
Fnu4HI GCNGC 3 cut(s) 320, 708, 854
FokI GGATG 5 cut(s) 92, 709, 730, 793, 800
Fsp4HI GCNGC 3 cut(s) 320, 708, 854
FspBI CTAG 3 cut(s) 384, 486, 862
GlaI GCGC 1 cut(s) 176
GluI GCNGC 3 cut(s) 320, 708, 854
HaeIII GGCC 1 cut(s) 564
HapII CCGG 1 cut(s) 399
HhaI GCGC 1 cut(s) 177
Hin1II CATG 2 cut(s) 696, 814
Hin6I GCGC 1 cut(s) 175
HinP1I GCGC 1 cut(s) 175
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HinfI GANTC 1 cut(s) 253
HpaII CCGG 1 cut(s) 399
Hpy166II GTNNAC 8 cut(s) 6, 45, 222, 368, 375, 377, 437, 571
Hpy188I TCNGA 4 cut(s) 73, 295, 314, 460
Hpy188III TCNNGA 3 cut(s) 55, 399, 509
Hpy8I GTNNAC 8 cut(s) 6, 45, 222, 368, 375, 377, 437, 571
Hpy99I CGWCG 1 cut(s) 13
HpyAV CCTTC 5 cut(s) 95, 420, 492, 691, 778
HpyCH4III ACNGT 3 cut(s) 372, 505, 625
HpyCH4IV ACGT 1 cut(s) 439
HpyCH4V TGCA 6 cut(s) 131, 322, 551, 648, 661, 856
HpyF10VI GCNNNNNNNGC 1 cut(s) 316
HpyF3I CTNAG 3 cut(s) 194, 459, 652
HpySE526I ACGT 1 cut(s) 439
Hsp92II CATG 2 cut(s) 696, 814
HspAI GCGC 1 cut(s) 175
KflI GGGWCCC 1 cut(s) 753
Kpn2I TCCGGA 1 cut(s) 398
LmnI GCTCC 2 cut(s) 315, 401
LpnPI CCDG 7 cut(s) 259, 263, 311, 393, 412, 696, 786
Lsp1109I GCAGC 3 cut(s) 306, 694, 840
LweI GCATC 2 cut(s) 657, 670
MaeI CTAG 3 cut(s) 384, 486, 862
MaeII ACGT 1 cut(s) 439
MaeIII GTNAC 1 cut(s) 713
MboII GAAGA 6 cut(s) 73, 278, 308, 457, 650, 814
MfeI CAATTG 1 cut(s) 558
MhlI GDGCHC 1 cut(s) 818
MlsI TGGCCA 1 cut(s) 564
MluCI AATT 3 cut(s) 84, 448, 558
MluNI TGGCCA 1 cut(s) 564
MmeI TCCRAC 2 cut(s) 51, 57
MnlI CCTC 6 cut(s) 127, 162, 300, 460, 751, 756
Mox20I TGGCCA 1 cut(s) 564
Mph1103I ATGCAT 1 cut(s) 650
MroI TCCGGA 1 cut(s) 398
MscI TGGCCA 1 cut(s) 564
MseI TTAA 2 cut(s) 611, 800
MslI CAYNNNNRTG 2 cut(s) 520, 775
Msp20I TGGCCA 1 cut(s) 564
MspA1I CMGCKG 1 cut(s) 656
MspI CCGG 1 cut(s) 399
MunI CAATTG 1 cut(s) 558
MwoI GCNNNNNNNGC 1 cut(s) 316
NlaIII CATG 2 cut(s) 696, 814
NlaIV GGNNCC 3 cut(s) 311, 754, 755
NsiI ATGCAT 1 cut(s) 650
NspI RCATGY 1 cut(s) 814
OliI CACNNNNGTG 1 cut(s) 520
PaqCI CACCTGC 1 cut(s) 388
PciI ACATGT 1 cut(s) 810
PfeI GAWTC 1 cut(s) 253
PkrI GCNGC 3 cut(s) 321, 709, 855
PpuMI RGGWCCY 1 cut(s) 753
PscI ACATGT 1 cut(s) 810
PsiI TTATAA 1 cut(s) 500
Psp5II RGGWCCY 1 cut(s) 753
PspN4I GGNNCC 3 cut(s) 311, 754, 755
PspPI GGNCC 2 cut(s) 219, 753
PspPPI RGGWCCY 1 cut(s) 753
PvuII CAGCTG 1 cut(s) 656
RsaI GTAC 5 cut(s) 44, 241, 248, 376, 734
RsaNI GTAC 5 cut(s) 43, 240, 247, 375, 733
RseI CAYNNNNRTG 2 cut(s) 520, 775
SaqAI TTAA 2 cut(s) 611, 800
SatI GCNGC 3 cut(s) 320, 708, 854
Sau96I GGNCC 2 cut(s) 219, 753
SduI GDGCHC 1 cut(s) 818
SfaNI GCATC 2 cut(s) 657, 670
SinI GGWCC 2 cut(s) 219, 753
SmiMI CAYNNNNRTG 2 cut(s) 520, 775
SmlI CTYRAG 1 cut(s) 758
SmoI CTYRAG 1 cut(s) 758
Sse9I AATT 3 cut(s) 84, 448, 558
SsiI CCGC 2 cut(s) 494, 736
SspMI CTAG 3 cut(s) 384, 486, 862
StyI CCWWGG 1 cut(s) 747
TaaI ACNGT 3 cut(s) 372, 505, 625
TaiI ACGT 1 cut(s) 442
TaqI TCGA 1 cut(s) 341
TasI AATT 3 cut(s) 84, 448, 558
TatI WGTACW 2 cut(s) 42, 374
TfiI GAWTC 1 cut(s) 253
Tru1I TTAA 2 cut(s) 611, 800
Tru9I TTAA 2 cut(s) 611, 800
TseI GCWGC 3 cut(s) 319, 707, 853
TspDTI ATGAA 4 cut(s) 96, 246, 713, 797
TspGWI ACGGA 1 cut(s) 26
VpaK11BI GGWCC 2 cut(s) 219, 753
XapI RAATTY 1 cut(s) 448
XceI RCATGY 1 cut(s) 814
XspI CTAG 3 cut(s) 384, 486, 862
Zsp2I ATGCAT 1 cut(s) 650
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.