MD11G1187600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0006259
Physical Location & Seq
Forward (+)
26398763 .. 26403736
4974 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1187600.v1.1.491

Sequence Viewer

Length: 870 bp
ATGTCGACCACAGAAGCGCTCTACTCCAAACTCTACGATAAGTACTCGAAAATCAAGACAAAGAAATGGTCGGAGTTGGAAGATATTGGTAAAGATCAAGAAGGAAAGTTCAAGCATTATGTATCTGTTGTGGAGGAGTATATTCAACATTTGCGAAATGAAAATGAGAGGTTGTGCTCGGAAGTTGGTGATCTGAGAAGTGAAGTTGCTTCTATTAGGTCCACCAAAGCTGAACAATGTACCGGGTACCAGAATCGTTTGTTGGAAGAGAACAGGAAAAATGAAAAGCTTTCTGAAGAAGTAGAGAGGCTCCGAAAGCTGCAACAGGAGGGAAATTTTAGCAGATCGAAAGGTAGCAACACAGACAATGGACTTTGTACACCTGCTAGTGGTCAAGTATCAGGAGCAGCAGGAAATCTCTCAAAGAGAAGACGGACAAGTAAGCGTAGGAAGAATTCCCAGTCTGAGGCGGAGGATTTAGTTATGCCTTCTAGTGTGGCTCTTGTAACTGTTCAAGAGCCACAATGGTGTAGAACTATTGAAAGACCAGGTTGTGCAAAACCAATTGGCCAAGTTAACTGCGTGTTTCAAGTGCTTGTTGAGTATATGGTGGATTTAAAGTTCTCGACTGTTAGTCAAGCTGAAGAAATATGCATCTCAGCTGTGCATCAATCAAGTGGTTACGCCTTCAATCTTACATGGATGAACAGAGCTGCTGGTAGCGAAGTAGAACTTCTGTATCACACCGCATCCTTGGGGACGCTTGAGAGGGAAGCACCAGAATGGATGAAGGATGACATAGTTTTAAGCACAAACATGTGCCCTATCTTCTTTGAAAGGTTATCTCGTGTTGTCAAGCTGCACTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

290

Amino Acids

32.83

Weight (kDa)

7.55

Isoelectric Point (pI)

54.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7806 PF25091 192 - 286 1.4e-42 Domain of unknown function (DUF7806)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 391
Acc36I ACCTGC 1 cut(s) 391
Acc65I GGTACC 1 cut(s) 246
AccB1I GGYRCC 1 cut(s) 246
AccI GTMKAC 1 cut(s) 5
AciI CCGC 2 cut(s) 470, 747
AcoI YGGCCR 1 cut(s) 568
AcsI RAATTY 2 cut(s) 334, 454
AcuI CTGAAG 2 cut(s) 315, 663
AfaI GTAC 4 cut(s) 44, 241, 248, 379
AfeI AGCGCT 1 cut(s) 18
AfiI CCNNNNNNNGG 2 cut(s) 389, 466
AflIII ACRYGT 1 cut(s) 816
AgsI TTSAA 7 cut(s) 112, 146, 515, 542, 590, 691, 836
AhdI GACNNNNNGTC 1 cut(s) 633
AjnI CCWGG 1 cut(s) 547
AjuI GAANNNNNNNTTGG 2 cut(s) 245, 277
AleI CACNNNNGTG 1 cut(s) 526
AloI GAACNNNNNNTCC 2 cut(s) 605, 637
AluBI AGCT 7 cut(s) 230, 289, 319, 641, 662, 713, 859
AluI AGCT 7 cut(s) 230, 289, 319, 641, 662, 713, 859
Alw21I GWGCWC 1 cut(s) 179
Aor51HI AGCGCT 1 cut(s) 18
AoxI GGCC 1 cut(s) 568
ApeKI GCWGC 4 cut(s) 319, 407, 713, 859
ApoI RAATTY 2 cut(s) 334, 454
ArsI GACNNNNNNTTYG 2 cut(s) 53, 85
Asp718I GGTACC 1 cut(s) 246
AspLEI GCGC 1 cut(s) 19
AspS9I GGNCC 1 cut(s) 219
AsuC2I CCSGG 1 cut(s) 244
AsuHPI GGTGA 1 cut(s) 200
AvaII GGWCC 1 cut(s) 219
BaeGI GKGCMC 1 cut(s) 824
BaeI ACNNNNGTAYC 2 cut(s) 722, 755
BalI TGGCCA 1 cut(s) 570
BanI GGYRCC 1 cut(s) 246
BauI CACGAG 1 cut(s) 846
BbsI GAAGAC 1 cut(s) 436
Bbv12I GWGCWC 1 cut(s) 179
BbvI GCAGC 4 cut(s) 306, 419, 700, 846
BciT130I CCWGG 1 cut(s) 549
BcnI CCSGG 1 cut(s) 244
BfaI CTAG 3 cut(s) 387, 492, 868
BfoI RGCGCY 1 cut(s) 20
BfuAI ACCTGC 1 cut(s) 391
BisI GCNGC 4 cut(s) 320, 408, 714, 860
BlsI GCNGC 4 cut(s) 321, 409, 715, 861
BmcAI AGTACT 1 cut(s) 44
Bme1390I CCNGG 2 cut(s) 244, 549
Bme18I GGWCC 1 cut(s) 219
BmeRI GACNNNNNGTC 1 cut(s) 633
BmgT120I GGNCC 1 cut(s) 219
BmiI GGNNCC 2 cut(s) 248, 311
BmrFI CCNGG 2 cut(s) 244, 549
BmrI ACTGGG 1 cut(s) 454
BmsI GCATC 3 cut(s) 663, 676, 758
BmuI ACTGGG 1 cut(s) 454
BpiI GAAGAC 1 cut(s) 436
BpuEI CTTGAG 1 cut(s) 785
BpuMI CCSGG 1 cut(s) 244
BsaJI CCNNGG 1 cut(s) 753
Bsc4I CCNNNNNNNGG 2 cut(s) 389, 466
Bse1I ACTGG 1 cut(s) 460
BseBI CCWGG 1 cut(s) 549
BseDI CCNNGG 1 cut(s) 753
BseGI GGATG 4 cut(s) 708, 749, 792, 799
BseLI CCNNNNNNNGG 2 cut(s) 389, 466
BseMII CTCAG 3 cut(s) 185, 456, 672
BseNI ACTGG 1 cut(s) 460
BseRI GAGGAG 1 cut(s) 149
BseSI GKGCMC 1 cut(s) 824
BseXI GCAGC 4 cut(s) 306, 419, 700, 846
BsgI GTGCAG 1 cut(s) 845
BshFI GGCC 1 cut(s) 570
BshNI GGYRCC 1 cut(s) 246
BsiHKAI GWGCWC 1 cut(s) 179
BsiSI CCGG 1 cut(s) 243
BslFI GGGAC 1 cut(s) 772
BslI CCNNNNNNNGG 2 cut(s) 389, 466
BsmFI GGGAC 1 cut(s) 772
BsnI GGCC 1 cut(s) 570
Bsp1286I GDGCHC 2 cut(s) 179, 824
Bsp1407I TGTACA 1 cut(s) 377
Bsp143I GATC 3 cut(s) 94, 190, 344
BspACI CCGC 2 cut(s) 470, 747
BspANI GGCC 1 cut(s) 570
BspCNI CTCAG 3 cut(s) 186, 457, 671
BspLI GGNNCC 2 cut(s) 248, 311
BspMI ACCTGC 1 cut(s) 391
BspT107I GGYRCC 1 cut(s) 246
BsrGI TGTACA 1 cut(s) 377
BsrI ACTGG 1 cut(s) 460
BssECI CCNNGG 1 cut(s) 753
BssMI GATC 3 cut(s) 94, 190, 344
BssSI CACGAG 1 cut(s) 846
BssT1I CCWWGG 1 cut(s) 753
Bst2BI CACGAG 1 cut(s) 846
Bst2UI CCWGG 1 cut(s) 549
Bst4CI ACNGT 2 cut(s) 511, 631
Bst6I CTCTTC 1 cut(s) 261
BstAUI TGTACA 1 cut(s) 377
BstDEI CTNAG 3 cut(s) 194, 465, 658
BstF5I GGATG 4 cut(s) 708, 749, 792, 799
BstH2I RGCGCY 1 cut(s) 20
BstHHI GCGC 1 cut(s) 19
BstKTI GATC 3 cut(s) 97, 193, 347
BstMBI GATC 3 cut(s) 94, 190, 344
BstMWI GCNNNNNNNGC 1 cut(s) 316
BstNI CCWGG 1 cut(s) 549
BstNSI RCATGY 1 cut(s) 820
BstSCI CCNGG 2 cut(s) 242, 547
BstSLI GKGCMC 1 cut(s) 824
BstV1I GCAGC 4 cut(s) 306, 419, 700, 846
BstV2I GAAGAC 1 cut(s) 436
BsuRI GGCC 1 cut(s) 570
BtsCI GGATG 4 cut(s) 708, 749, 792, 799
BveI ACCTGC 1 cut(s) 391
CfoI GCGC 1 cut(s) 19
Cfr13I GGNCC 1 cut(s) 219
CseI GACGC 1 cut(s) 769
CsiI ACCWGGT 1 cut(s) 547
Csp6I GTAC 4 cut(s) 43, 240, 247, 378
CviAII CATG 2 cut(s) 699, 817
CviQI GTAC 4 cut(s) 43, 240, 247, 378
DdeI CTNAG 3 cut(s) 194, 465, 658
DpnI GATC 3 cut(s) 96, 192, 346
DpnII GATC 3 cut(s) 94, 190, 344
DraI TTTAAA 1 cut(s) 618
DriI GACNNNNNGTC 1 cut(s) 633
EaeI YGGCCR 1 cut(s) 568
Eam1104I CTCTTC 1 cut(s) 261
Eam1105I GACNNNNNGTC 1 cut(s) 633
EarI CTCTTC 1 cut(s) 261
EciI GGCGGA 1 cut(s) 485
Eco130I CCWWGG 1 cut(s) 753
Eco47I GGWCC 1 cut(s) 219
Eco47III AGCGCT 1 cut(s) 18
Eco57I CTGAAG 2 cut(s) 315, 663
EcoRI GAATTC 1 cut(s) 454
EcoRII CCWGG 1 cut(s) 547
EcoT14I CCWWGG 1 cut(s) 753
EcoT22I ATGCAT 1 cut(s) 656
ErhI CCWWGG 1 cut(s) 753
FaeI CATG 2 cut(s) 702, 820
FaiI YATR 9 cut(s) 120, 141, 485, 606, 608, 652, 700, 800, 818
FalI AAGNNNNNCTT 2 cut(s) 717, 749
FaqI GGGAC 1 cut(s) 772
FatI CATG 2 cut(s) 698, 816
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 4 cut(s) 320, 408, 714, 860
FokI GGATG 4 cut(s) 715, 736, 799, 806
Fsp4HI GCNGC 4 cut(s) 320, 408, 714, 860
FspBI CTAG 3 cut(s) 387, 492, 868
GlaI GCGC 1 cut(s) 18
GluI GCNGC 4 cut(s) 320, 408, 714, 860
HaeII RGCGCY 1 cut(s) 20
HaeIII GGCC 1 cut(s) 570
HapII CCGG 1 cut(s) 243
HgaI GACGC 1 cut(s) 769
HhaI GCGC 1 cut(s) 19
Hin1II CATG 2 cut(s) 702, 820
Hin6I GCGC 1 cut(s) 17
HinP1I GCGC 1 cut(s) 17
HincII GTYRAC 2 cut(s) 6, 577
HindII GTYRAC 2 cut(s) 6, 577
HindIII AAGCTT 1 cut(s) 287
HinfI GANTC 1 cut(s) 253
HpaI GTTAAC 1 cut(s) 577
HpaII CCGG 1 cut(s) 243
HphI GGTGA 1 cut(s) 200
Hpy166II GTNNAC 4 cut(s) 6, 222, 380, 577
Hpy188I TCNGA 6 cut(s) 73, 181, 195, 295, 314, 466
Hpy188III TCNNGA 5 cut(s) 55, 98, 402, 515, 625
Hpy8I GTNNAC 4 cut(s) 6, 222, 380, 577
HpyAV CCTTC 4 cut(s) 95, 498, 697, 784
HpyCH4III ACNGT 2 cut(s) 511, 631
HpyCH4V TGCA 5 cut(s) 322, 557, 654, 667, 862
HpyF10VI GCNNNNNNNGC 1 cut(s) 316
HpyF3I CTNAG 3 cut(s) 194, 465, 658
Hsp92II CATG 2 cut(s) 702, 820
HspAI GCGC 1 cut(s) 17
KpnI GGTACC 1 cut(s) 250
KspAI GTTAAC 1 cut(s) 577
Kzo9I GATC 3 cut(s) 94, 190, 344
LmnI GCTCC 2 cut(s) 315, 404
Lsp1109I GCAGC 4 cut(s) 306, 419, 700, 846
LweI GCATC 3 cut(s) 663, 676, 758
MabI ACCWGGT 1 cut(s) 547
MaeI CTAG 3 cut(s) 387, 492, 868
MaeIII GTNAC 2 cut(s) 505, 680
MalI GATC 3 cut(s) 96, 192, 346
MboI GATC 3 cut(s) 94, 190, 344
MboII GAAGA 7 cut(s) 92, 278, 308, 441, 463, 656, 820
MfeI CAATTG 1 cut(s) 564
MhlI GDGCHC 2 cut(s) 179, 824
MlsI TGGCCA 1 cut(s) 570
MluCI AATT 3 cut(s) 334, 454, 564
MluNI TGGCCA 1 cut(s) 570
MmeI TCCRAC 3 cut(s) 51, 57, 243
MnlI CCTC 7 cut(s) 127, 162, 300, 322, 460, 466, 762
Mox20I TGGCCA 1 cut(s) 570
Mph1103I ATGCAT 1 cut(s) 656
MscI TGGCCA 1 cut(s) 570
MseI TTAA 3 cut(s) 576, 617, 806
MslI CAYNNNNRTG 3 cut(s) 526, 781, 815
Msp20I TGGCCA 1 cut(s) 570
MspA1I CMGCKG 1 cut(s) 662
MspI CCGG 1 cut(s) 243
MspR9I CCNGG 2 cut(s) 244, 549
MunI CAATTG 1 cut(s) 564
MvaI CCWGG 1 cut(s) 549
MwoI GCNNNNNNNGC 1 cut(s) 316
NciI CCSGG 1 cut(s) 244
NdeII GATC 3 cut(s) 94, 190, 344
NlaIII CATG 2 cut(s) 702, 820
NlaIV GGNNCC 2 cut(s) 248, 311
NsiI ATGCAT 1 cut(s) 656
NspI RCATGY 1 cut(s) 820
OliI CACNNNNGTG 1 cut(s) 526
PaqCI CACCTGC 1 cut(s) 391
PciI ACATGT 1 cut(s) 816
PfeI GAWTC 1 cut(s) 253
PkrI GCNGC 4 cut(s) 321, 409, 715, 861
PscI ACATGT 1 cut(s) 816
Psp6I CCWGG 1 cut(s) 547
PspGI CCWGG 1 cut(s) 547
PspN4I GGNNCC 2 cut(s) 248, 311
PspPI GGNCC 1 cut(s) 219
PvuII CAGCTG 1 cut(s) 662
RsaI GTAC 4 cut(s) 44, 241, 248, 379
RsaNI GTAC 4 cut(s) 43, 240, 247, 378
RseI CAYNNNNRTG 3 cut(s) 526, 781, 815
SalI GTCGAC 1 cut(s) 4
SaqAI TTAA 3 cut(s) 576, 617, 806
SatI GCNGC 4 cut(s) 320, 408, 714, 860
Sau3AI GATC 3 cut(s) 94, 190, 344
Sau96I GGNCC 1 cut(s) 219
ScaI AGTACT 1 cut(s) 44
ScrFI CCNGG 2 cut(s) 244, 549
SduI GDGCHC 2 cut(s) 179, 824
SexAI ACCWGGT 1 cut(s) 547
SfaNI GCATC 3 cut(s) 663, 676, 758
SinI GGWCC 1 cut(s) 219
SmiMI CAYNNNNRTG 3 cut(s) 526, 781, 815
SmlI CTYRAG 1 cut(s) 764
SmoI CTYRAG 1 cut(s) 764
Sse9I AATT 3 cut(s) 334, 454, 564
SsiI CCGC 2 cut(s) 470, 747
SspMI CTAG 3 cut(s) 387, 492, 868
StyD4I CCNGG 2 cut(s) 242, 547
StyI CCWWGG 1 cut(s) 753
TaaI ACNGT 2 cut(s) 511, 631
TaqI TCGA 4 cut(s) 5, 47, 347, 626
TasI AATT 3 cut(s) 334, 454, 564
TatI WGTACW 2 cut(s) 42, 377
TfiI GAWTC 1 cut(s) 253
Tru1I TTAA 3 cut(s) 576, 617, 806
Tru9I TTAA 3 cut(s) 576, 617, 806
TseI GCWGC 4 cut(s) 319, 407, 713, 859
TspDTI ATGAA 4 cut(s) 174, 297, 719, 803
TspGWI ACGGA 1 cut(s) 448
VpaK11BI GGWCC 1 cut(s) 219
XapI RAATTY 2 cut(s) 334, 454
XceI RCATGY 1 cut(s) 820
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 3 cut(s) 387, 492, 868
ZrmI AGTACT 1 cut(s) 44
Zsp2I ATGCAT 1 cut(s) 656
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.