Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0007580

Physical Location & Seq

Genomic Coordinates Reverse (-)

24304857 .. 24309631

Gene Structure 4775 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1176800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1245 bp

ATGATGAGCGCTCAGCCTCAGCCGCCGAGTAGTATGATGGCGGCGGCTCCTACCACGTCTTCTCAGGGGTACCAGCATCATCATCAGCCAACTTCAGTGGAGGAGGTCAGAACCCTCTGGATTGGGGATTTACAGTATTGGGTGGACGAAAACTACCTCAATACCTGCTTTGCTCACACCGGCGAGGTGATTTCAATAAAGATAATACGTAATAAAATCACTGGCCAGCCGGAGGGTTATGGGTTTGTGGAATTTGTTTCTCATGCTGCAGCGGAAAGGGTTCTGCATGCTTACAATGGGACACAAATGCCTGGAACTGAACAAACTTTTAGGCTAAACTGGGCTTCCTTTGGTATTGGGGAAAGACGTCCTGATGCTGGGCCTGAGCACTCTATTTTTGTAGGCGATTTAGCCCCTGATGTTACAGATTATATGCTGCAAGAAACCTTTCGAACTAATTATCCTGAAAGGAACCGAGCTATGTCAGAAATGAATGGTCAGTATTGCTCAAGTAGACCGATGCGTATCAGTGCAGCAACACCGAAGAAAACCACTGGTTTTCAGCAACAATATGCTGCCGCAAAAGCGGTATATCCTGTCCCAGCATACACTACACCAGCAGTTCCCGTGCTTCCTACAGCAGATTATGACGTAAACAACACCACTATTTTTGTTGGTAACTTGGATCCTAATGTGGCGGAGGATGAACTCAAGCAAACTTTTATGCAGTTTGGAGATATTTTCTATGTCAAGATTCCTGTCGGCAAAGGATGTGGTTTTGTACAGTTTGGGACAAGGGCATCTGCTGAAGAAGCTATCCAAAGGATGCAGGGAAAGATGATCGGTCAACAAGTAGTCCGTATTTCTTGGGGCAGGAGCCCAACAGCTAAACAGGACGTACCTGGTACCTGGGGTCAGCAAGCAGATCCAAACCAATGGGCTGCATATTATGGTTATGGACAAGGCTACGATGCCTATGCTTACGGGGCGGCGACTCCTGATCCATCATTATATGCATATGGTGCATATGCTGGCTATGGCCAATATCCTCAACAGGTTGAAGGTCAAGATGCTGCAACTGCGGGAGGTGCTATCCCATCTGTTGAACAGAGAGAAGAGTTGGATGATCCCTTGGCTACACCGGACGTTGATAAGTTAAATGCAGCTTACCTCGCCCGCCACGAAAGGGCCATCTTATGCCGGCCTTTGTGGCTGAGAACATCATCATTTACGCAGCAAGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006950 GO:0008143 GO:0008150 GO:0009266 GO:0009408 GO:0009628 GO:0009987 GO:0010494 GO:0032991 GO:0033554 GO:0034605 GO:0035770 GO:0036464 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0051716 GO:0070717 GO:0097159 GO:1901363 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RRM_1

Protein Analysis

415

Amino Acids

45.75

Weight (kDa)

5.24

Isoelectric Point (pI)

38.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RRM_1	PF00076	39 - 107	4.8e-14	RNA recognition motif
RRM_1	PF00076	223 - 286	2.8e-17	RNA recognition motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007580)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G19350 AT5G19350
fragaria_vesca	FvH4_1g30440 FvH4_1g30440 FvH4_1g30440
malus_domestica	MD03G1176800.v1.1 MD11G1197600.v1.1
prunus_persica	Prupe.5G028700_v2.0.a1 Prupe.5G028700_v2.0.a1
pyrus_communis	pycom03g13400 pycom11g17020
rosa_chinensis	RchiOBHm_Chr3g0491021
rosa_laevigata	RLG00000022869 RLG00000022873
rosa_multiflora	Rmu_sc0009408.1_g000001 Rmu_sc0019624.1_g000001 Rmu_ssc0000386.1_g000038
rosa_roxburghii	Rroxscaffold_6G00393280
rosa_rugosa	Rorug03G0245500 Rorug04G0070300
rosa_samantha	Rh3AG295500 Rh3BG331400 Rh3CG328900
rosa_wichuraiana	Rw3G026080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	370
Acc36I	ACCTGC	1 cut(s)	173
Acc65I	GGTACC	2 cut(s)	69, 905
AccB1I	GGYRCC	2 cut(s)	69, 905
AccI	GTMKAC	1 cut(s)	514
AclWI	GGATC	5 cut(s)	680, 693, 920, 995, 1121
AcoI	YGGCCR	2 cut(s)	223, 1039
AcsI	RAATTY	1 cut(s)	251
AcuI	CTGAAG	2 cut(s)	78, 828
AcyI	GRCGYC	1 cut(s)	367
AfaI	GTAC	4 cut(s)	71, 783, 900, 907
AfeI	AGCGCT	1 cut(s)	10
AfiI	CCNNNNNNNGG	3 cut(s)	232, 377, 1186
AgsI	TTSAA	3 cut(s)	195, 1061, 1106
AjiI	CACGTC	1 cut(s)	57
AjnI	CCWGG	3 cut(s)	310, 901, 908
AloI	GAACNNNNNNTCC	2 cut(s)	445, 477
AluBI	AGCT	5 cut(s)	479, 815, 887, 1166, 1241
AluI	AGCT	5 cut(s)	479, 815, 887, 1166, 1241
Alw21I	GWGCWC	1 cut(s)	390
AlwI	GGATC	5 cut(s)	680, 693, 920, 995, 1121
Aor51HI	AGCGCT	1 cut(s)	10
AoxI	GGCC	5 cut(s)	223, 380, 1039, 1188, 1202
ApeKI	GCWGC	9 cut(s)	266, 269, 436, 533, 575, 941, 1073, 1163, 1234
ApoI	RAATTY	1 cut(s)	251
Asp700I	GAANNNNTTC	2 cut(s)	279, 447
Asp718I	GGTACC	2 cut(s)	69, 905
AspLEI	GCGC	1 cut(s)	11
AspS9I	GGNCC	2 cut(s)	380, 1188
AsuHPI	GGTGA	1 cut(s)	199
AsuII	TTCGAA	1 cut(s)	451
BalI	TGGCCA	2 cut(s)	225, 1041
BamHI	GGATCC	1 cut(s)	685
BanI	GGYRCC	2 cut(s)	69, 905
BanII	GRGCYC	1 cut(s)	881
BbsI	GAAGAC	1 cut(s)	51
Bbv12I	GWGCWC	1 cut(s)	390
BbvCI	CCTCAGC	1 cut(s)	18
BbvI	GCAGC	8 cut(s)	253, 281, 423, 545, 562, 928, 1060, 1175
BccI	CCATC	4 cut(s)	31, 1012, 1105, 1199
BcgI	CGANNNNNNTGC	2 cut(s)	959, 993
BciT130I	CCWGG	3 cut(s)	312, 903, 910
BfmI	CTRYAG	2 cut(s)	267, 636
BfoI	RGCGCY	1 cut(s)	12
BfuAI	ACCTGC	1 cut(s)	173
BglI	GCCNNNNNGGC	1 cut(s)	1210
BlpI	GCTNAGC	1 cut(s)	12
Bme1390I	CCNGG	3 cut(s)	312, 903, 910
BmgBI	CACGTC	1 cut(s)	57
BmgT120I	GGNCC	2 cut(s)	380, 1188
BmiI	GGNNCC	6 cut(s)	48, 71, 473, 687, 878, 907
BmrFI	CCNGG	3 cut(s)	312, 903, 910
BmrI	ACTGGG	1 cut(s)	349
BmsI	GCATC	7 cut(s)	85, 364, 510, 809, 816, 961, 1060
BmuI	ACTGGG	1 cut(s)	349
BpiI	GAAGAC	1 cut(s)	51
Bpu10I	CCTNAGC	2 cut(s)	18, 384
Bpu1102I	GCTNAGC	1 cut(s)	12
Bpu14I	TTCGAA	1 cut(s)	451
BpuEI	CTTGAG	2 cut(s)	493, 695
BsaAI	YACGTR	1 cut(s)	209
BsaBI	GATNNNNATC	1 cut(s)	524
BsaHI	GRCGYC	1 cut(s)	367
BsaJI	CCNNGG	2 cut(s)	909, 1131
BsaWI	WCCGGW	1 cut(s)	1141
Bsc4I	CCNNNNNNNGG	3 cut(s)	232, 377, 1186
Bse118I	RCCGGY	2 cut(s)	179, 1200
Bse1I	ACTGG	3 cut(s)	226, 344, 559
Bse8I	GATNNNNATC	1 cut(s)	524
BseBI	CCWGG	3 cut(s)	312, 903, 910
BseDI	CCNNGG	2 cut(s)	909, 1131
BseGI	GGATG	4 cut(s)	709, 776, 831, 1129
BseJI	GATNNNNATC	1 cut(s)	524
BseLI	CCNNNNNNNGG	3 cut(s)	232, 377, 1186
BseMII	CTCAG	5 cut(s)	26, 32, 77, 375, 1205
BseNI	ACTGG	3 cut(s)	226, 344, 559
BseRI	GAGGAG	1 cut(s)	116
BseXI	GCAGC	8 cut(s)	253, 281, 423, 545, 562, 928, 1060, 1175
BseYI	CCCAGC	2 cut(s)	377, 601
BsgI	GTGCAG	1 cut(s)	552
BshFI	GGCC	5 cut(s)	225, 382, 1041, 1190, 1204
BshNI	GGYRCC	2 cut(s)	69, 905
BsiHKAI	GWGCWC	1 cut(s)	390
BsiSI	CCGG	4 cut(s)	180, 230, 1142, 1201
BslFI	GGGAC	3 cut(s)	313, 584, 805
BslI	CCNNNNNNNGG	3 cut(s)	232, 377, 1186
BsmFI	GGGAC	3 cut(s)	313, 584, 805
BsnI	GGCC	5 cut(s)	225, 382, 1041, 1190, 1204
Bsp119I	TTCGAA	1 cut(s)	451
Bsp1286I	GDGCHC	2 cut(s)	390, 881
Bsp1407I	TGTACA	1 cut(s)	781
Bsp143I	GATC	5 cut(s)	685, 840, 925, 1000, 1126
Bsp1720I	GCTNAGC	1 cut(s)	12
BspANI	GGCC	5 cut(s)	225, 382, 1041, 1190, 1204
BspCNI	CTCAG	5 cut(s)	25, 31, 76, 376, 1206
BspLI	GGNNCC	6 cut(s)	48, 71, 473, 687, 878, 907
BspMAI	CTGCAG	1 cut(s)	271
BspMI	ACCTGC	1 cut(s)	173
BspPI	GGATC	5 cut(s)	680, 693, 920, 995, 1121
BspT104I	TTCGAA	1 cut(s)	451
BspT107I	GGYRCC	2 cut(s)	69, 905
BsrFI	RCCGGY	2 cut(s)	179, 1200
BsrGI	TGTACA	1 cut(s)	781
BsrI	ACTGG	3 cut(s)	226, 344, 559
BssAI	RCCGGY	2 cut(s)	179, 1200
BssECI	CCNNGG	2 cut(s)	909, 1131
BssMI	GATC	5 cut(s)	685, 840, 925, 1000, 1126
BssNI	GRCGYC	1 cut(s)	367
BssT1I	CCWWGG	1 cut(s)	1131
Bst2UI	CCWGG	3 cut(s)	312, 903, 910
Bst4CI	ACNGT	2 cut(s)	135, 786
Bst6I	CTCTTC	1 cut(s)	1110
BstACI	GRCGYC	1 cut(s)	367
BstAPI	GCANNNNNTGC	1 cut(s)	1022
BstAUI	TGTACA	1 cut(s)	781
BstBAI	YACGTR	1 cut(s)	209
BstBI	TTCGAA	1 cut(s)	451
BstC8I	GCNNGC	7 cut(s)	227, 288, 921, 1033, 1177, 1202, 1239
BstDEI	CTNAG	6 cut(s)	12, 18, 63, 384, 1214, 1242
BstF5I	GGATG	4 cut(s)	709, 776, 831, 1129
BstH2I	RGCGCY	1 cut(s)	12
BstHHI	GCGC	1 cut(s)	11
BstKTI	GATC	5 cut(s)	688, 843, 928, 1003, 1129
BstMBI	GATC	5 cut(s)	685, 840, 925, 1000, 1126
BstMWI	GCNNNNNNNGC	9 cut(s)	22, 584, 812, 986, 1022, 1079, 1088, 1172, 1210
BstNI	CCWGG	3 cut(s)	312, 903, 910
BstNSI	RCATGY	1 cut(s)	290
BstSCI	CCNGG	3 cut(s)	310, 901, 908
BstSFI	CTRYAG	2 cut(s)	267, 636
BstSNI	TACGTA	1 cut(s)	209
BstV1I	GCAGC	8 cut(s)	253, 281, 423, 545, 562, 928, 1060, 1175
BstV2I	GAAGAC	1 cut(s)	51
BstX2I	RGATCY	2 cut(s)	685, 925
BstXI	CCANNNNNNTGG	1 cut(s)	936
BstYI	RGATCY	2 cut(s)	685, 925
BsuRI	GGCC	5 cut(s)	225, 382, 1041, 1190, 1204
BtrI	CACGTC	1 cut(s)	57
BtsCI	GGATG	4 cut(s)	709, 776, 831, 1129
BtsIMutI	CAGTG	4 cut(s)	102, 219, 535, 552
BveI	ACCTGC	1 cut(s)	173
Cac8I	GCNNGC	7 cut(s)	227, 288, 921, 1033, 1177, 1202, 1239
CfoI	GCGC	1 cut(s)	11
Cfr10I	RCCGGY	2 cut(s)	179, 1200
Cfr13I	GGNCC	2 cut(s)	380, 1188
CsiI	ACCWGGT	1 cut(s)	901
Csp6I	GTAC	4 cut(s)	70, 782, 899, 906
CspCI	CAANNNNNGTGG	6 cut(s)	78, 113, 652, 687, 754, 789
CviAII	CATG	2 cut(s)	263, 287
CviQI	GTAC	4 cut(s)	70, 782, 899, 906
DdeI	CTNAG	6 cut(s)	12, 18, 63, 384, 1214, 1242
DpnI	GATC	5 cut(s)	687, 842, 927, 1002, 1128
DpnII	GATC	5 cut(s)	685, 840, 925, 1000, 1126
EaeI	YGGCCR	2 cut(s)	223, 1039
Eam1104I	CTCTTC	1 cut(s)	1110
EarI	CTCTTC	1 cut(s)	1110
EciI	GGCGGA	1 cut(s)	713
Eco105I	TACGTA	1 cut(s)	209
Eco130I	CCWWGG	1 cut(s)	1131
Eco24I	GRGCYC	1 cut(s)	881
Eco47III	AGCGCT	1 cut(s)	10
Eco57I	CTGAAG	2 cut(s)	78, 828
EcoRII	CCWGG	3 cut(s)	310, 901, 908
EcoT14I	CCWWGG	1 cut(s)	1131
EcoT22I	ATGCAT	1 cut(s)	1018
EcoT38I	GRGCYC	1 cut(s)	881
ErhI	CCWWGG	1 cut(s)	1131
FaeI	CATG	2 cut(s)	266, 290
FaqI	GGGAC	3 cut(s)	313, 584, 805
FatI	CATG	2 cut(s)	262, 286
FauI	CCCGC	2 cut(s)	1075, 1184
FauNDI	CATATG	2 cut(s)	1018, 1027
FblI	GTMKAC	1 cut(s)	514
FokI	GGATG	4 cut(s)	716, 783, 838, 1136
FriOI	GRGCYC	1 cut(s)	881
GlaI	GCGC	1 cut(s)	10
GsaI	CCCAGC	2 cut(s)	381, 605
HaeII	RGCGCY	1 cut(s)	12
HaeIII	GGCC	5 cut(s)	225, 382, 1041, 1190, 1204
HapII	CCGG	4 cut(s)	180, 230, 1142, 1201
HhaI	GCGC	1 cut(s)	11
Hin1I	GRCGYC	1 cut(s)	367
Hin1II	CATG	2 cut(s)	266, 290
Hin6I	GCGC	1 cut(s)	9
HinP1I	GCGC	1 cut(s)	9
HincII	GTYRAC	1 cut(s)	848
HindII	GTYRAC	1 cut(s)	848
HindIII	AAGCTT	1 cut(s)	1239
HinfI	GANTC	2 cut(s)	754, 994
HpaII	CCGG	4 cut(s)	180, 230, 1142, 1201
HphI	GGTGA	1 cut(s)	199
Hpy166II	GTNNAC	4 cut(s)	145, 515, 655, 848
Hpy188I	TCNGA	2 cut(s)	110, 487
Hpy188III	TCNNGA	6 cut(s)	118, 371, 464, 751, 998, 1067
Hpy8I	GTNNAC	4 cut(s)	145, 515, 655, 848
HpyAV	CCTTC	1 cut(s)	1055
HpyCH4III	ACNGT	2 cut(s)	135, 786
HpyCH4IV	ACGT	6 cut(s)	56, 208, 367, 651, 897, 1146
HpyF10VI	GCNNNNNNNGC	9 cut(s)	22, 584, 812, 986, 1022, 1079, 1088, 1172, 1210
HpyF3I	CTNAG	6 cut(s)	12, 18, 63, 384, 1214, 1242
HpySE526I	ACGT	6 cut(s)	56, 208, 367, 651, 897, 1146
Hsp92I	GRCGYC	1 cut(s)	367
Hsp92II	CATG	2 cut(s)	266, 290
HspAI	GCGC	1 cut(s)	9
KpnI	GGTACC	2 cut(s)	73, 909
KroI	GCCGGC	1 cut(s)	1200
KroNI	GCCGGC	1 cut(s)	1202
Kzo9I	GATC	5 cut(s)	685, 840, 925, 1000, 1126
LmnI	GCTCC	2 cut(s)	52, 876
Lsp1109I	GCAGC	8 cut(s)	253, 281, 423, 545, 562, 928, 1060, 1175
LweI	GCATC	7 cut(s)	85, 364, 510, 809, 816, 961, 1060
MabI	ACCWGGT	1 cut(s)	901
MaeII	ACGT	6 cut(s)	56, 208, 367, 651, 897, 1146
MaeIII	GTNAC	2 cut(s)	421, 677
MalI	GATC	5 cut(s)	687, 842, 927, 1002, 1128
MboI	GATC	5 cut(s)	685, 840, 925, 1000, 1126
MboII	GAAGA	4 cut(s)	51, 556, 821, 1127
MflI	RGATCY	2 cut(s)	685, 925
MhlI	GDGCHC	2 cut(s)	390, 881
MlsI	TGGCCA	2 cut(s)	225, 1041
MluCI	AATT	2 cut(s)	251, 457
MluNI	TGGCCA	2 cut(s)	225, 1041
MlyI	GAGTC	1 cut(s)	988
MmeI	TCCRAC	1 cut(s)	1101
Mox20I	TGGCCA	2 cut(s)	225, 1041
Mph1103I	ATGCAT	1 cut(s)	1018
MroNI	GCCGGC	1 cut(s)	1200
MroXI	GAANNNNTTC	2 cut(s)	279, 447
MscI	TGGCCA	2 cut(s)	225, 1041
MseI	TTAA	1 cut(s)	1157
Msp20I	TGGCCA	2 cut(s)	225, 1041
MspA1I	CMGCKG	1 cut(s)	272
MspI	CCGG	4 cut(s)	180, 230, 1142, 1201
MspR9I	CCNGG	3 cut(s)	312, 903, 910
MvaI	CCWGG	3 cut(s)	312, 903, 910
MwoI	GCNNNNNNNGC	9 cut(s)	22, 584, 812, 986, 1022, 1079, 1088, 1172, 1210
NaeI	GCCGGC	1 cut(s)	1202
NdeI	CATATG	2 cut(s)	1018, 1027
NdeII	GATC	5 cut(s)	685, 840, 925, 1000, 1126
NgoMIV	GCCGGC	1 cut(s)	1200
NlaIII	CATG	2 cut(s)	266, 290
NlaIV	GGNNCC	6 cut(s)	48, 71, 473, 687, 878, 907
NmeAIII	GCCGAG	1 cut(s)	51
NsiI	ATGCAT	1 cut(s)	1018
NspI	RCATGY	1 cut(s)	290
NspV	TTCGAA	1 cut(s)	451
PaeI	GCATGC	1 cut(s)	290
PcsI	WCGNNNNNNNCGW	1 cut(s)	1179
PdiI	GCCGGC	1 cut(s)	1202
PdmI	GAANNNNTTC	2 cut(s)	279, 447
PfeI	GAWTC	1 cut(s)	754
PleI	GAGTC	1 cut(s)	988
PpsI	GAGTC	1 cut(s)	988
Ppu21I	YACGTR	1 cut(s)	209
Psp6I	CCWGG	3 cut(s)	310, 901, 908
PspFI	CCCAGC	2 cut(s)	377, 601
PspGI	CCWGG	3 cut(s)	310, 901, 908
PspN4I	GGNNCC	6 cut(s)	48, 71, 473, 687, 878, 907
PspPI	GGNCC	2 cut(s)	380, 1188
PstI	CTGCAG	1 cut(s)	271
PsuI	RGATCY	2 cut(s)	685, 925
RsaI	GTAC	4 cut(s)	71, 783, 900, 907
RsaNI	GTAC	4 cut(s)	70, 782, 899, 906
SaqAI	TTAA	1 cut(s)	1157
Sau3AI	GATC	5 cut(s)	685, 840, 925, 1000, 1126
Sau96I	GGNCC	2 cut(s)	380, 1188
SchI	GAGTC	1 cut(s)	988
ScrFI	CCNGG	3 cut(s)	312, 903, 910
SduI	GDGCHC	2 cut(s)	390, 881
SexAI	ACCWGGT	1 cut(s)	901
SfaNI	GCATC	7 cut(s)	85, 364, 510, 809, 816, 961, 1060
SfcI	CTRYAG	2 cut(s)	267, 636
SfuI	TTCGAA	1 cut(s)	451
SgrAI	CRCCGGYG	1 cut(s)	179
SmlI	CTYRAG	2 cut(s)	508, 710
SmoI	CTYRAG	2 cut(s)	508, 710
SnaBI	TACGTA	1 cut(s)	209
SphI	GCATGC	1 cut(s)	290
Sse9I	AATT	2 cut(s)	251, 457
StyD4I	CCNGG	3 cut(s)	310, 901, 908
StyI	CCWWGG	1 cut(s)	1131
TaaI	ACNGT	2 cut(s)	135, 786
TaiI	ACGT	6 cut(s)	59, 211, 370, 654, 900, 1149
TaqI	TCGA	1 cut(s)	451
TaqII	GACCGA	2 cut(s)	532, 833
TasI	AATT	2 cut(s)	251, 457
TatI	WGTACW	1 cut(s)	781
TauI	GCSGC	5 cut(s)	25, 44, 47, 581, 992
TfiI	GAWTC	1 cut(s)	754
Tru1I	TTAA	1 cut(s)	1157
Tru9I	TTAA	1 cut(s)	1157
TscAI	CASTG	4 cut(s)	102, 226, 535, 559
TseI	GCWGC	9 cut(s)	266, 269, 436, 533, 575, 941, 1073, 1163, 1234
TspDTI	ATGAA	2 cut(s)	506, 720
TspGWI	ACGGA	1 cut(s)	848
TspRI	CASTG	4 cut(s)	102, 226, 535, 559
XapI	RAATTY	1 cut(s)	251
XceI	RCATGY	1 cut(s)	290
XmiI	GTMKAC	1 cut(s)	514
XmnI	GAANNNNTTC	2 cut(s)	279, 447
ZraI	GACGTC	1 cut(s)	368
Zsp2I	ATGCAT	1 cut(s)	1018

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.