Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0013643

Physical Location & Seq

Genomic Coordinates Forward (+)

27003520 .. 27006281

Gene Structure 2762 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1197600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1617 bp

ATGGAATCTTCAGCTAGTGATCATTCTATTCACAACTGCAGATTCGTACTTTCTTCTGAGACGACGAATGATCACGATCATGGGAAACGAGTGGTCGGTGAATTGGACTTCTTTGCTGATAAGGGCCGTTTGAAAGAAGATAGAGATCACACGGCTGAAGTAAAAGAAGAATGCGATCGTCACGGGGTTGGTCAAGAAAAGCAACTACCCGACGTAAATATTGGATTGAATCTTCTTACAACAAACACCAGTAGCGAAAAATCATCCATGGATGATGGAACTTCGTCTTCCCATACTATGGAAAACAAGCATAGAACAAATCAGCTGGCCGTTCTTCGAGCTGAATTAGGCCATATGAACGTAGAAAACCAACGGTTGAGAGGTGCGATTCATCAGTTAAACACTCATTACCAGGCGTTACAGGTGCACCTTGTGACACTGATGCAACGCCAGAAAAATCAAAAGACTGATTATCAAACTGCAGAACAACACAAGATGAACATAAATGGATCAGTGGTACTAGAAGAGAAGCAGATGATGAACGGGATTAATAATCTTGCTCCAATACAATTTATGGATATGGGAAGGGCTGAGAAAGATGAGCTTTCACAGTGTTCTCATGAAGGCTGCAGATCCCAAGATTGTCCCGGGTCGCCCCCTCGAAACGACATCGTCGAGTCAATGGAGTGCTGCAAGAGTACTAGTCATGTTCACAGGGATATTAGTGGAAGAAGTCGTACTGCTGGGGGGGAAGATAGCCCTGACCAAGAACTTCAAGGGTGGATTCCTAAGAAGGTTCCCAAGTTGATAAGCCCTAGGGAAGTGGATCAACCCTCATCTGAAGCCATGTCCATGATTAAAAAAGCTCGTGTTTCAGTTCGAGCACGATCTGAAGCTTCCATGATATCTGATGGATGCCAATGGAGAAAGTATGGCCAGAAAATGGCTAAAGGAAACCCATGCCCTCGAGCTTATTATCGTTGCACCATGAGAAATGGTTGCCCGGTTCGCAAACAGGTACAGAGATGTGCGGAAGATCGAACAATACTTATAACAACATACGAAGGCCACCACAACCATCCACTCCCTCCTGCTGCAATGGCCATGGCATCTACTACATCAGCATCAGCATCAATGATTCTATCAGGTTCAATGCCTAGTGCTGATGGCCTAATAAGCTCAAACAGCTTCCTAGCAAGAACTGGATTGCCTAACTACCCACCAAGCTTGGCAACACTTTCGGCCTCAGCCCCATTTCCTACTGTCACCTTGGACCTCACTAACACTCCCACTTCCTCAGAGATGCCACTTGGCCAACCAAACCAGCTCCCTCCAAATTTTCCTCACAACAATCTTATGTCTGTGCCACAAATTCTAGGTCAAGCCCTTTGCAACCAATCCAAATTCTCGGTTCTCGATAGCCTTCAGGGGTTGGATAGTACTACACACTCACTGGCTGACAAAGTCAGTGCAGCAACAGCGGCCATCACAGCGGACCCTAATTTCACAGCGGCTCTGGTAGCAGCCATCACCTCTATTGTTGTCAATGGTCATTCAAACAATAACACCTACAACAATGTTGCTACAAGAAACAATAGTGACGACAATACAGAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0006355 GO:0006950 GO:0007154 GO:0008150 GO:0009267 GO:0009605 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0009991 GO:0010033 GO:0010036 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016036 GO:0019219 GO:0019222 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031667 GO:0031668 GO:0031669 GO:0033554 GO:0042221 GO:0042493 GO:0042594 GO:0044212 GO:0045892 GO:0045934 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0071496 GO:0080029 GO:0080090 GO:0080169 GO:0097159 GO:0140110 GO:1901363 GO:1901698 GO:1901700 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WRKY

Protein Analysis

539

Amino Acids

58.93

Weight (kDa)

6.43

Isoelectric Point (pI)

44.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRKY	PF03106	304 - 361	1.3e-25	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013643)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g23150
malus_domestica	MD03G1197600.v1.1 MD11G1213500.v1.1
prunus_persica	Prupe.4G217900_v2.0.a1
pyrus_communis	pycom03g14910 pycom11g18810 pycom12g06880
rosa_chinensis	RchiOBHm_Chr5g0040801
rosa_laevigata	RLG00000034002
rosa_multiflora	Rmu_sc0001579.1_g000002
rosa_roxburghii	Rroxscaffold_1G00039900
rosa_rugosa	Rorug05G0187400 Rorug05G0187500
rosa_samantha	Rh5AG273100 Rh5BG278200 Rh5CG310200 Rh5DG286100
rosa_wichuraiana	Rw5G025680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1052
AccB7I	CCANNNNNTGG	2 cut(s)	298, 943
AciI	CCGC	4 cut(s)	1031, 1481, 1493, 1511
AclWI	GGATC	3 cut(s)	517, 627, 834
AcoI	YGGCCR	5 cut(s)	327, 934, 1101, 1312, 1482
AcsI	RAATTY	3 cut(s)	1336, 1371, 1403
AcuI	CTGAAG	4 cut(s)	177, 861, 912, 1409
AdeI	CACNNNGTG	1 cut(s)	433
AfaI	GTAC	6 cut(s)	48, 519, 700, 739, 1020, 1441
AfiI	CCNNNNNNNGG	2 cut(s)	298, 943
AgsI	TTSAA	5 cut(s)	133, 229, 776, 1152, 1557
AhlI	ACTAGT	1 cut(s)	701
AjnI	CCWGG	1 cut(s)	411
Alw21I	GWGCWC	2 cut(s)	429, 886
Alw26I	GTCTC	1 cut(s)	53
Alw44I	GTGCAC	1 cut(s)	425
AlwI	GGATC	3 cut(s)	517, 627, 834
Ama87I	CYCGRG	2 cut(s)	647, 966
ApaLI	GTGCAC	1 cut(s)	425
ApeKI	GCWGC	5 cut(s)	627, 690, 1094, 1472, 1523
ApoI	RAATTY	3 cut(s)	1336, 1371, 1403
ArsI	GACNNNNNNTTYG	2 cut(s)	1363, 1395
AseI	ATTAAT	1 cut(s)	549
AspA2I	CCTAGG	1 cut(s)	815
AspS9I	GGNCC	3 cut(s)	124, 1273, 1495
AsuC2I	CCSGG	3 cut(s)	648, 649, 1004
AsuHPI	GGTGA	3 cut(s)	110, 1258, 1522
AvaI	CYCGRG	2 cut(s)	647, 966
AvaII	GGWCC	2 cut(s)	1273, 1495
AvrII	CCTAGG	1 cut(s)	815
BaeGI	GKGCMC	1 cut(s)	429
BalI	TGGCCA	3 cut(s)	936, 1103, 1314
BarI	GAAGNNNNNNTAC	2 cut(s)	721, 753
BauI	CACGAG	1 cut(s)	867
BbsI	GAAGAC	1 cut(s)	279
Bbv12I	GWGCWC	2 cut(s)	429, 886
BbvCI	CCTCAGC	1 cut(s)	1246
BbvI	GCAGC	5 cut(s)	614, 677, 1081, 1484, 1535
BccI	CCATC	6 cut(s)	269, 905, 1086, 1160, 1493, 1535
BceAI	ACGGC	3 cut(s)	111, 168, 314
BcgI	CGANNNNNNTGC	2 cut(s)	1221, 1255
BciT130I	CCWGG	1 cut(s)	413
BclI	TGATCA	2 cut(s)	19, 70
BcnI	CCSGG	3 cut(s)	648, 649, 1004
BcoDI	GTCTC	1 cut(s)	53
BcuI	ACTAGT	1 cut(s)	701
BfaI	CTAG	7 cut(s)	15, 521, 702, 816, 1158, 1193, 1376
BfmI	CTRYAG	3 cut(s)	37, 480, 628
BisI	GCNGC	7 cut(s)	628, 691, 1095, 1473, 1482, 1512, 1524
BlnI	CCTAGG	1 cut(s)	815
BlsI	GCNGC	7 cut(s)	629, 692, 1096, 1474, 1483, 1513, 1525
BmcAI	AGTACT	2 cut(s)	700, 1441
Bme1390I	CCNGG	4 cut(s)	413, 648, 649, 1004
Bme18I	GGWCC	2 cut(s)	1273, 1495
BmeT110I	CYCGRG	2 cut(s)	647, 966
BmgT120I	GGNCC	3 cut(s)	124, 1273, 1495
BmiI	GGNNCC	2 cut(s)	798, 1497
BmrFI	CCNGG	4 cut(s)	413, 648, 649, 1004
BmsI	GCATC	6 cut(s)	432, 905, 1118, 1133, 1139, 1293
BpiI	GAAGAC	1 cut(s)	279
Bpu10I	CCTNAGC	1 cut(s)	1246
BpuMI	CCSGG	3 cut(s)	648, 649, 1004
BsaBI	GATNNNNATC	2 cut(s)	75, 144
BsaJI	CCNNGG	5 cut(s)	267, 647, 815, 1104, 1269
BsaXI	ACNNNNNCTCC	2 cut(s)	1270, 1300
Bsc4I	CCNNNNNNNGG	2 cut(s)	298, 943
Bse1I	ACTGG	3 cut(s)	249, 1207, 1458
Bse3DI	GCAATG	1 cut(s)	1104
Bse8I	GATNNNNATC	2 cut(s)	75, 144
BseBI	CCWGG	1 cut(s)	413
BseDI	CCNNGG	5 cut(s)	267, 647, 815, 1104, 1269
BseGI	GGATG	4 cut(s)	263, 277, 920, 1078
BseJI	GATNNNNATC	2 cut(s)	75, 144
BseLI	CCNNNNNNNGG	2 cut(s)	298, 943
BseMI	GCAATG	1 cut(s)	1104
BseMII	CTCAG	4 cut(s)	48, 582, 1260, 1311
BseNI	ACTGG	3 cut(s)	249, 1207, 1458
BseSI	GKGCMC	1 cut(s)	429
BseXI	GCAGC	5 cut(s)	614, 677, 1081, 1484, 1535
BseYI	CCCAGC	1 cut(s)	743
BsgI	GTGCAG	1 cut(s)	1491
Bsh1285I	CGRYCG	1 cut(s)	178
BsiEI	CGRYCG	1 cut(s)	178
BsiHKAI	GWGCWC	2 cut(s)	429, 886
BsiHKCI	CYCGRG	2 cut(s)	647, 966
BsiSI	CCGG	2 cut(s)	648, 1004
BslFI	GGGAC	1 cut(s)	630
BslI	CCNNNNNNNGG	2 cut(s)	298, 943
BsmAI	GTCTC	1 cut(s)	53
BsmBI	CGTCTC	1 cut(s)	53
BsmFI	GGGAC	1 cut(s)	630
BsmI	GAATGC	1 cut(s)	176
BsoBI	CYCGRG	2 cut(s)	647, 966
Bsp1286I	GDGCHC	2 cut(s)	429, 886
Bsp19I	CCATGG	2 cut(s)	267, 1104
BspACI	CCGC	4 cut(s)	1031, 1481, 1493, 1511
BspCNI	CTCAG	4 cut(s)	49, 583, 1259, 1310
BspHI	TCATGA	1 cut(s)	619
BspLI	GGNNCC	2 cut(s)	798, 1497
BspMAI	CTGCAG	3 cut(s)	41, 484, 632
BspPI	GGATC	3 cut(s)	517, 627, 834
BsrDI	GCAATG	1 cut(s)	1104
BsrI	ACTGG	3 cut(s)	249, 1207, 1458
BssECI	CCNNGG	5 cut(s)	267, 647, 815, 1104, 1269
BssSI	CACGAG	1 cut(s)	867
BssT1I	CCWWGG	4 cut(s)	267, 815, 1104, 1269
Bst2BI	CACGAG	1 cut(s)	867
Bst2UI	CCWGG	1 cut(s)	413
Bst4CI	ACNGT	3 cut(s)	375, 612, 1264
Bst6I	CTCTTC	1 cut(s)	519
BstC8I	GCNNGC	1 cut(s)	327
BstDEI	CTNAG	5 cut(s)	57, 591, 789, 1246, 1297
BstDSI	CCRYGG	2 cut(s)	267, 1104
BstF5I	GGATG	4 cut(s)	263, 277, 920, 1078
BstMAI	GTCTC	1 cut(s)	53
BstMCI	CGRYCG	1 cut(s)	178
BstMWI	GCNNNNNNNGC	8 cut(s)	1008, 1100, 1176, 1185, 1478, 1481, 1490, 1520
BstNI	CCWGG	1 cut(s)	413
BstSCI	CCNGG	4 cut(s)	411, 646, 647, 1002
BstSFI	CTRYAG	3 cut(s)	37, 480, 628
BstSLI	GKGCMC	1 cut(s)	429
BstV1I	GCAGC	5 cut(s)	614, 677, 1081, 1484, 1535
BstV2I	GAAGAC	1 cut(s)	279
BstX2I	RGATCY	1 cut(s)	632
BstYI	RGATCY	1 cut(s)	632
BtgI	CCRYGG	2 cut(s)	267, 1104
BtsCI	GGATG	4 cut(s)	263, 277, 920, 1078
BtsIMutI	CAGTG	5 cut(s)	437, 519, 617, 1451, 1474
Cac8I	GCNNGC	1 cut(s)	327
CciI	TCATGA	1 cut(s)	619
Cfr13I	GGNCC	3 cut(s)	124, 1273, 1495
Cfr9I	CCCGGG	1 cut(s)	647
Csp6I	GTAC	6 cut(s)	47, 518, 699, 738, 1019, 1440
CspCI	CAANNNNNGTGG	2 cut(s)	1209, 1244
CviQI	GTAC	6 cut(s)	47, 518, 699, 738, 1019, 1440
DdeI	CTNAG	5 cut(s)	57, 591, 789, 1246, 1297
DraIII	CACNNNGTG	1 cut(s)	433
EaeI	YGGCCR	5 cut(s)	327, 934, 1101, 1312, 1482
Eam1104I	CTCTTC	1 cut(s)	519
EarI	CTCTTC	1 cut(s)	519
Eco130I	CCWWGG	4 cut(s)	267, 815, 1104, 1269
Eco32I	GATATC	1 cut(s)	906
Eco47I	GGWCC	2 cut(s)	1273, 1495
Eco57I	CTGAAG	4 cut(s)	177, 861, 912, 1409
Eco88I	CYCGRG	2 cut(s)	647, 966
EcoRII	CCWGG	1 cut(s)	411
EcoRV	GATATC	1 cut(s)	906
EcoT14I	CCWWGG	4 cut(s)	267, 815, 1104, 1269
ErhI	CCWWGG	4 cut(s)	267, 815, 1104, 1269
Esp3I	CGTCTC	1 cut(s)	53
FalI	AAGNNNNNCTT	2 cut(s)	588, 620
FaqI	GGGAC	1 cut(s)	630
FauNDI	CATATG	1 cut(s)	354
FbaI	TGATCA	2 cut(s)	19, 70
Fnu4HI	GCNGC	7 cut(s)	628, 691, 1095, 1473, 1482, 1512, 1524
FokI	GGATG	4 cut(s)	250, 284, 927, 1065
Fsp4HI	GCNGC	7 cut(s)	628, 691, 1095, 1473, 1482, 1512, 1524
FspBI	CTAG	7 cut(s)	15, 521, 702, 816, 1158, 1193, 1376
GluI	GCNGC	7 cut(s)	628, 691, 1095, 1473, 1482, 1512, 1524
GsaI	CCCAGC	1 cut(s)	747
HapII	CCGG	2 cut(s)	648, 1004
HindIII	AAGCTT	2 cut(s)	894, 1225
HinfI	GANTC	7 cut(s)	5, 42, 229, 388, 677, 784, 1138
HpaII	CCGG	2 cut(s)	648, 1004
HphI	GGTGA	3 cut(s)	110, 1258, 1522
Hpy166II	GTNNAC	2 cut(s)	427, 712
Hpy188I	TCNGA	5 cut(s)	58, 841, 892, 910, 1300
Hpy188III	TCNNGA	4 cut(s)	74, 194, 620, 1415
Hpy8I	GTNNAC	2 cut(s)	427, 712
Hpy99I	CGWCG	3 cut(s)	67, 215, 677
HpyAV	CCTTC	5 cut(s)	579, 617, 787, 1058, 1433
HpyCH4III	ACNGT	3 cut(s)	375, 612, 1264
HpyCH4IV	ACGT	2 cut(s)	213, 360
HpyF10VI	GCNNNNNNNGC	8 cut(s)	1008, 1100, 1176, 1185, 1478, 1481, 1490, 1520
HpyF3I	CTNAG	5 cut(s)	57, 591, 789, 1246, 1297
HpySE526I	ACGT	2 cut(s)	213, 360
Ksp22I	TGATCA	2 cut(s)	19, 70
LmnI	GCTCC	2 cut(s)	565, 1332
Lsp1109I	GCAGC	5 cut(s)	614, 677, 1081, 1484, 1535
LweI	GCATC	6 cut(s)	432, 905, 1118, 1133, 1139, 1293
MaeI	CTAG	7 cut(s)	15, 521, 702, 816, 1158, 1193, 1376
MaeII	ACGT	2 cut(s)	213, 360
MaeIII	GTNAC	5 cut(s)	179, 417, 433, 1264, 1598
MflI	RGATCY	1 cut(s)	632
MhlI	GDGCHC	2 cut(s)	429, 886
MlsI	TGGCCA	3 cut(s)	936, 1103, 1314
MluCI	AATT	7 cut(s)	101, 344, 569, 1336, 1371, 1403, 1501
MluNI	TGGCCA	3 cut(s)	936, 1103, 1314
MlyI	GAGTC	1 cut(s)	686
MmeI	TCCRAC	1 cut(s)	1413
Mox20I	TGGCCA	3 cut(s)	936, 1103, 1314
MscI	TGGCCA	3 cut(s)	936, 1103, 1314
MseI	TTAA	3 cut(s)	398, 549, 858
MslI	CAYNNNNRTG	2 cut(s)	78, 851
Msp20I	TGGCCA	3 cut(s)	936, 1103, 1314
MspA1I	CMGCKG	4 cut(s)	325, 1481, 1493, 1511
MspI	CCGG	2 cut(s)	648, 1004
MspR9I	CCNGG	4 cut(s)	413, 648, 649, 1004
Mva1269I	GAATGC	1 cut(s)	176
MvaI	CCWGG	1 cut(s)	413
MwoI	GCNNNNNNNGC	8 cut(s)	1008, 1100, 1176, 1185, 1478, 1481, 1490, 1520
NciI	CCSGG	3 cut(s)	648, 649, 1004
NcoI	CCATGG	2 cut(s)	267, 1104
NdeI	CATATG	1 cut(s)	354
NlaIV	GGNNCC	2 cut(s)	798, 1497
NmuCI	GTSAC	4 cut(s)	179, 433, 1264, 1598
PaeR7I	CTCGAG	1 cut(s)	966
PagI	TCATGA	1 cut(s)	619
PcsI	WCGNNNNNNNCGW	1 cut(s)	672
PctI	GAATGC	1 cut(s)	176
PfeI	GAWTC	6 cut(s)	5, 42, 229, 388, 784, 1138
PflFI	GACNNNGTC	2 cut(s)	671, 1463
PflMI	CCANNNNNTGG	2 cut(s)	298, 943
PkrI	GCNGC	7 cut(s)	629, 692, 1096, 1474, 1483, 1513, 1525
Ple19I	CGATCG	1 cut(s)	178
PleI	GAGTC	1 cut(s)	685
PpsI	GAGTC	1 cut(s)	685
PshBI	ATTAAT	1 cut(s)	549
PsiI	TTATAA	1 cut(s)	1052
Psp6I	CCWGG	1 cut(s)	411
PspFI	CCCAGC	1 cut(s)	743
PspGI	CCWGG	1 cut(s)	411
PspN4I	GGNNCC	2 cut(s)	798, 1497
PspPI	GGNCC	3 cut(s)	124, 1273, 1495
PspXI	VCTCGAGB	1 cut(s)	966
PstI	CTGCAG	3 cut(s)	41, 484, 632
PsuI	RGATCY	1 cut(s)	632
PsyI	GACNNNGTC	2 cut(s)	671, 1463
PvuI	CGATCG	1 cut(s)	178
PvuII	CAGCTG	1 cut(s)	325
RsaI	GTAC	6 cut(s)	48, 519, 700, 739, 1020, 1441
RsaNI	GTAC	6 cut(s)	47, 518, 699, 738, 1019, 1440
RseI	CAYNNNNRTG	2 cut(s)	78, 851
SaqAI	TTAA	3 cut(s)	398, 549, 858
SatI	GCNGC	7 cut(s)	628, 691, 1095, 1473, 1482, 1512, 1524
Sau96I	GGNCC	3 cut(s)	124, 1273, 1495
ScaI	AGTACT	2 cut(s)	700, 1441
SchI	GAGTC	1 cut(s)	686
ScrFI	CCNGG	4 cut(s)	413, 648, 649, 1004
SduI	GDGCHC	2 cut(s)	429, 886
SfaNI	GCATC	6 cut(s)	432, 905, 1118, 1133, 1139, 1293
SfcI	CTRYAG	3 cut(s)	37, 480, 628
Sfr274I	CTCGAG	1 cut(s)	966
SinI	GGWCC	2 cut(s)	1273, 1495
SlaI	CTCGAG	1 cut(s)	966
SmaI	CCCGGG	1 cut(s)	649
SmiMI	CAYNNNNRTG	2 cut(s)	78, 851
SmlI	CTYRAG	1 cut(s)	966
SmoI	CTYRAG	1 cut(s)	966
SpeI	ACTAGT	1 cut(s)	701
Sse9I	AATT	7 cut(s)	101, 344, 569, 1336, 1371, 1403, 1501
SsiI	CCGC	4 cut(s)	1031, 1481, 1493, 1511
SspI	AATATT	1 cut(s)	220
SspMI	CTAG	7 cut(s)	15, 521, 702, 816, 1158, 1193, 1376
StyD4I	CCNGG	4 cut(s)	411, 646, 647, 1002
StyI	CCWWGG	4 cut(s)	267, 815, 1104, 1269
TaaI	ACNGT	3 cut(s)	375, 612, 1264
TaiI	ACGT	2 cut(s)	216, 363
TaqI	TCGA	7 cut(s)	337, 661, 675, 880, 967, 1039, 1416
TasI	AATT	7 cut(s)	101, 344, 569, 1336, 1371, 1403, 1501
TatI	WGTACW	2 cut(s)	698, 1439
TauI	GCSGC	2 cut(s)	1484, 1514
TfiI	GAWTC	6 cut(s)	5, 42, 229, 388, 784, 1138
Tru1I	TTAA	3 cut(s)	398, 549, 858
Tru9I	TTAA	3 cut(s)	398, 549, 858
TscAI	CASTG	5 cut(s)	444, 519, 617, 1458, 1474
TseFI	GTSAC	4 cut(s)	179, 433, 1264, 1598
TseI	GCWGC	5 cut(s)	627, 690, 1094, 1472, 1523
Tsp45I	GTSAC	4 cut(s)	179, 433, 1264, 1598
TspDTI	ATGAA	5 cut(s)	371, 380, 512, 554, 636
TspMI	CCCGGG	1 cut(s)	647
TspRI	CASTG	5 cut(s)	444, 519, 617, 1458, 1474
Tth111I	GACNNNGTC	2 cut(s)	671, 1463
Van91I	CCANNNNNTGG	2 cut(s)	298, 943
VneI	GTGCAC	1 cut(s)	425
VpaK11BI	GGWCC	2 cut(s)	1273, 1495
VspI	ATTAAT	1 cut(s)	549
XapI	RAATTY	3 cut(s)	1336, 1371, 1403
XhoI	CTCGAG	1 cut(s)	966
XmaI	CCCGGG	1 cut(s)	647
XmaJI	CCTAGG	1 cut(s)	815
XspI	CTAG	7 cut(s)	15, 521, 702, 816, 1158, 1193, 1376
ZrmI	AGTACT	2 cut(s)	700, 1441

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.