pycom11g18810
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0013643
Physical Location & Seq
Forward (+)
21036069 .. 21037837
1769 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g18810.1

Sequence Viewer

Length: 1077 bp
ATGAACGAGTTCAATAATCTTGCTCCAAGACAATTTATGGATATGGGAAGGGCTCAGAAAGATGAGCTATCACAGTGTTCTCATGAAGGCTGCAGATCCCAAGATTGTTCTGGGTCGTCCCCTCGAAACGACATAGTCGAGTCAATGGAGTGTTGCAAGGGTACTAGTCATGTTCACAGGGATATTAGTGGAAAAAGTAGTACTACTGGGGCAGAAGATAGCCCTGACCATGAATTTCAAGGGTGGATTCCTAAGAAGGTTCCCAAGTTGATGAGCCCTAGGGAAGTGGAACAAGCCTCATCCGAAACCATGTCCATGATTAAAAAAGCTCGGGTTTCTGTTCGAGCACGATCTGAGGCTTCCATGATGTCTGATGGATGCCAATGGAGAAAGTATGGCCAGAAAATGGCTAAAGGAAACCCATGCCCTCGAGCTTATTATCGTTGCACTATGGCAGCTGGTTGCCCGGTTCGCAAACAGGTACAAAGATGTGCGGAAGATCGAACAATACTTATAACAACATACGAAGGTCACCACAACCATCCACTCCCTCCAGCAGCAATGGCCATGGCATCTACTACATCAGCAGCAGCATCAATGATTCTATCAGGTTCAAATCATAGTGCTGATGGCCTAATAAGCTCAAACAGCTTCCTCGCAAGAACAGGACTGCCTAACTGCCCACCAAGCTTGGCAACACTTTCGGCTACAGCCCCATTTCCTACTGTCACCTTGGACCTCACTCACACTCCCACTTCCTCAGAGATGCCACTTGGCCAACCAAACCAGCTCCCTTCAAATTTTCCCCACAACAATCTTATGTCTGTGCCACAAATTCTAGGTCAAGCCCTTTGTAACCAACCTAAATTCTCGGTTCTTGATAGCCTTCAAGGGTTGGATAGTACTACACACTCACTGGCTGACAAAGTCAATGCAGCAACAGCAGCCATCACAGCTGACCCTAATTTCACAATGGCTCTGGTAGCAGCTATCACCTCTATCGTTGGCAATGTTCAATCAAACAATAACACCAACAACAATGTTTCTCCAAGACACGATAGTGGCAACAGTACATGA

Protein Analysis

359

Amino Acids

38.44

Weight (kDa)

7.58

Isoelectric Point (pI)

46.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 125 - 182 7.6e-26 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013643)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g23150
malus_domestica MD03G1197600.v1.1 MD11G1213500.v1.1
prunus_persica Prupe.4G217900_v2.0.a1
pyrus_communis pycom03g14910 pycom11g18810 pycom12g06880
rosa_chinensis RchiOBHm_Chr5g0040801
rosa_laevigata RLG00000034002
rosa_multiflora Rmu_sc0001579.1_g000002
rosa_roxburghii Rroxscaffold_1G00039900
rosa_rugosa Rorug05G0187400 Rorug05G0187500
rosa_samantha Rh5AG273100 Rh5BG278200 Rh5CG310200 Rh5DG286100
rosa_wichuraiana Rw5G025680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 515
AccB7I CCANNNNNTGG 1 cut(s) 406
AciI CCGC 1 cut(s) 494
AclWI GGATC 1 cut(s) 90
AcoI YGGCCR 3 cut(s) 397, 564, 775
AcsI RAATTY 4 cut(s) 233, 799, 834, 866
AfaI GTAC 5 cut(s) 163, 202, 483, 904, 1072
AfiI CCNNNNNNNGG 1 cut(s) 406
AgsI TTSAA 6 cut(s) 13, 239, 615, 798, 890, 1016
AhlI ACTAGT 1 cut(s) 164
AleI CACNNNNGTG 1 cut(s) 1059
Alw21I GWGCWC 1 cut(s) 349
AlwI GGATC 1 cut(s) 90
Ama87I CYCGRG 2 cut(s) 330, 429
AoxI GGCC 4 cut(s) 397, 564, 631, 775
ApeKI GCWGC 8 cut(s) 90, 455, 557, 587, 590, 935, 944, 986
ApoI RAATTY 4 cut(s) 233, 799, 834, 866
ArsI GACNNNNNNTTYG 2 cut(s) 826, 858
Asp700I GAANNNNTTC 1 cut(s) 8
AspA2I CCTAGG 1 cut(s) 278
AspS9I GGNCC 1 cut(s) 736
AsuC2I CCSGG 1 cut(s) 467
AsuHPI GGTGA 3 cut(s) 524, 721, 985
AvaI CYCGRG 2 cut(s) 330, 429
AvaII GGWCC 1 cut(s) 736
AvrII CCTAGG 1 cut(s) 278
BalI TGGCCA 3 cut(s) 399, 566, 777
BanII GRGCYC 2 cut(s) 55, 278
Bbv12I GWGCWC 1 cut(s) 349
BbvI GCAGC 8 cut(s) 77, 467, 569, 599, 602, 947, 956, 998
BccI CCATC 4 cut(s) 368, 549, 623, 956
BcgI CGANNNNNNTGC 2 cut(s) 684, 718
BcnI CCSGG 1 cut(s) 467
BcuI ACTAGT 1 cut(s) 164
BfaI CTAG 3 cut(s) 165, 279, 839
BfmI CTRYAG 2 cut(s) 91, 708
BisI GCNGC 8 cut(s) 91, 456, 558, 588, 591, 936, 945, 987
BlnI CCTAGG 1 cut(s) 278
BlsI GCNGC 8 cut(s) 92, 457, 559, 589, 592, 937, 946, 988
BmcAI AGTACT 2 cut(s) 202, 904
Bme1390I CCNGG 1 cut(s) 467
Bme18I GGWCC 1 cut(s) 736
BmeT110I CYCGRG 2 cut(s) 330, 429
BmgT120I GGNCC 1 cut(s) 736
BmiI GGNNCC 1 cut(s) 261
BmrFI CCNGG 1 cut(s) 467
BmrI ACTGGG 1 cut(s) 216
BmsI GCATC 4 cut(s) 368, 581, 602, 756
BmuI ACTGGG 1 cut(s) 216
BpmI CTGGAG 1 cut(s) 537
BpuMI CCSGG 1 cut(s) 467
BsaJI CCNNGG 3 cut(s) 278, 567, 732
BsaXI ACNNNNNCTCC 2 cut(s) 733, 763
Bsc4I CCNNNNNNNGG 1 cut(s) 406
Bse1I ACTGG 2 cut(s) 211, 921
Bse3DI GCAATG 2 cut(s) 567, 1015
BseDI CCNNGG 3 cut(s) 278, 567, 732
BseGI GGATG 3 cut(s) 299, 383, 541
BseLI CCNNNNNNNGG 1 cut(s) 406
BseMI GCAATG 2 cut(s) 567, 1015
BseMII CTCAG 3 cut(s) 68, 345, 774
BseNI ACTGG 2 cut(s) 211, 921
BseXI GCAGC 8 cut(s) 77, 467, 569, 599, 602, 947, 956, 998
BshFI GGCC 4 cut(s) 399, 566, 633, 777
BsiHKAI GWGCWC 1 cut(s) 349
BsiHKCI CYCGRG 2 cut(s) 330, 429
BsiSI CCGG 1 cut(s) 467
BslFI GGGAC 1 cut(s) 103
BslI CCNNNNNNNGG 1 cut(s) 406
BsmFI GGGAC 1 cut(s) 103
BsnI GGCC 4 cut(s) 399, 566, 633, 777
BsoBI CYCGRG 2 cut(s) 330, 429
Bsp1286I GDGCHC 3 cut(s) 55, 278, 349
Bsp143I GATC 3 cut(s) 95, 350, 499
Bsp19I CCATGG 1 cut(s) 567
BspACI CCGC 1 cut(s) 494
BspANI GGCC 4 cut(s) 399, 566, 633, 777
BspCNI CTCAG 3 cut(s) 67, 346, 773
BspHI TCATGA 1 cut(s) 82
BspLI GGNNCC 1 cut(s) 261
BspMAI CTGCAG 1 cut(s) 95
BspPI GGATC 1 cut(s) 90
BsrDI GCAATG 2 cut(s) 567, 1015
BsrI ACTGG 2 cut(s) 211, 921
BssECI CCNNGG 3 cut(s) 278, 567, 732
BssMI GATC 3 cut(s) 95, 350, 499
BssT1I CCWWGG 3 cut(s) 278, 567, 732
Bst4CI ACNGT 3 cut(s) 75, 727, 1070
BstDEI CTNAG 4 cut(s) 54, 252, 354, 760
BstDSI CCRYGG 1 cut(s) 567
BstEII GGTNACC 1 cut(s) 530
BstF5I GGATG 3 cut(s) 299, 383, 541
BstKTI GATC 3 cut(s) 98, 353, 502
BstMBI GATC 3 cut(s) 95, 350, 499
BstMWI GCNNNNNNNGC 9 cut(s) 471, 563, 639, 648, 687, 941, 944, 953, 983
BstPI GGTNACC 1 cut(s) 530
BstSCI CCNGG 1 cut(s) 465
BstSFI CTRYAG 2 cut(s) 91, 708
BstV1I GCAGC 8 cut(s) 77, 467, 569, 599, 602, 947, 956, 998
BstX2I RGATCY 1 cut(s) 95
BstYI RGATCY 1 cut(s) 95
BsuRI GGCC 4 cut(s) 399, 566, 633, 777
BtgI CCRYGG 1 cut(s) 567
BtsCI GGATG 3 cut(s) 299, 383, 541
BtsIMutI CAGTG 2 cut(s) 80, 914
CciI TCATGA 1 cut(s) 82
Cfr13I GGNCC 1 cut(s) 736
Csp6I GTAC 5 cut(s) 162, 201, 482, 903, 1071
CspCI CAANNNNNGTGG 2 cut(s) 672, 707
CviAII CATG 9 cut(s) 83, 170, 230, 310, 316, 364, 423, 568, 1074
CviQI GTAC 5 cut(s) 162, 201, 482, 903, 1071
DdeI CTNAG 4 cut(s) 54, 252, 354, 760
DpnI GATC 3 cut(s) 97, 352, 501
DpnII GATC 3 cut(s) 95, 350, 499
EaeI YGGCCR 3 cut(s) 397, 564, 775
Eco130I CCWWGG 3 cut(s) 278, 567, 732
Eco24I GRGCYC 2 cut(s) 55, 278
Eco47I GGWCC 1 cut(s) 736
Eco88I CYCGRG 2 cut(s) 330, 429
Eco91I GGTNACC 1 cut(s) 530
EcoO65I GGTNACC 1 cut(s) 530
EcoT14I CCWWGG 3 cut(s) 278, 567, 732
EcoT38I GRGCYC 2 cut(s) 55, 278
ErhI CCWWGG 3 cut(s) 278, 567, 732
FaeI CATG 9 cut(s) 86, 173, 233, 313, 319, 367, 426, 571, 1077
FaqI GGGAC 1 cut(s) 103
FatI CATG 9 cut(s) 82, 169, 229, 309, 315, 363, 422, 567, 1073
Fnu4HI GCNGC 8 cut(s) 91, 456, 558, 588, 591, 936, 945, 987
FokI GGATG 3 cut(s) 286, 390, 528
FriOI GRGCYC 2 cut(s) 55, 278
Fsp4HI GCNGC 8 cut(s) 91, 456, 558, 588, 591, 936, 945, 987
FspBI CTAG 3 cut(s) 165, 279, 839
GluI GCNGC 8 cut(s) 91, 456, 558, 588, 591, 936, 945, 987
GsuI CTGGAG 1 cut(s) 537
HaeIII GGCC 4 cut(s) 399, 566, 633, 777
HapII CCGG 1 cut(s) 467
Hin1II CATG 9 cut(s) 86, 173, 233, 313, 319, 367, 426, 571, 1077
HindIII AAGCTT 1 cut(s) 688
HinfI GANTC 3 cut(s) 140, 247, 601
HpaII CCGG 1 cut(s) 467
HphI GGTGA 3 cut(s) 524, 721, 985
Hpy166II GTNNAC 1 cut(s) 175
Hpy188I TCNGA 5 cut(s) 57, 304, 355, 373, 763
Hpy188III TCNNGA 2 cut(s) 83, 878
Hpy8I GTNNAC 1 cut(s) 175
HpyAV CCTTC 6 cut(s) 42, 80, 250, 521, 804, 896
HpyCH4III ACNGT 3 cut(s) 75, 727, 1070
HpyCH4V TGCA 4 cut(s) 93, 156, 447, 935
HpyF10VI GCNNNNNNNGC 9 cut(s) 471, 563, 639, 648, 687, 941, 944, 953, 983
HpyF3I CTNAG 4 cut(s) 54, 252, 354, 760
Hsp92II CATG 9 cut(s) 86, 173, 233, 313, 319, 367, 426, 571, 1077
Kzo9I GATC 3 cut(s) 95, 350, 499
LmnI GCTCC 2 cut(s) 28, 795
Lsp1109I GCAGC 8 cut(s) 77, 467, 569, 599, 602, 947, 956, 998
LweI GCATC 4 cut(s) 368, 581, 602, 756
MaeI CTAG 3 cut(s) 165, 279, 839
MaeIII GTNAC 3 cut(s) 530, 727, 854
MalI GATC 3 cut(s) 97, 352, 501
MboI GATC 3 cut(s) 95, 350, 499
MboII GAAGA 2 cut(s) 227, 509
MflI RGATCY 1 cut(s) 95
MhlI GDGCHC 3 cut(s) 55, 278, 349
MlsI TGGCCA 3 cut(s) 399, 566, 777
MluCI AATT 6 cut(s) 32, 233, 799, 834, 866, 964
MluNI TGGCCA 3 cut(s) 399, 566, 777
MlyI GAGTC 1 cut(s) 149
MmeI TCCRAC 1 cut(s) 876
MnlI CCTC 9 cut(s) 132, 307, 349, 438, 561, 665, 749, 769, 1006
Mox20I TGGCCA 3 cut(s) 399, 566, 777
MroXI GAANNNNTTC 1 cut(s) 8
MscI TGGCCA 3 cut(s) 399, 566, 777
MseI TTAA 1 cut(s) 321
MslI CAYNNNNRTG 2 cut(s) 314, 1059
Msp20I TGGCCA 3 cut(s) 399, 566, 777
MspA1I CMGCKG 2 cut(s) 458, 956
MspI CCGG 1 cut(s) 467
MspR9I CCNGG 1 cut(s) 467
MwoI GCNNNNNNNGC 9 cut(s) 471, 563, 639, 648, 687, 941, 944, 953, 983
NciI CCSGG 1 cut(s) 467
NcoI CCATGG 1 cut(s) 567
NdeII GATC 3 cut(s) 95, 350, 499
NlaIII CATG 9 cut(s) 86, 173, 233, 313, 319, 367, 426, 571, 1077
NlaIV GGNNCC 1 cut(s) 261
NmuCI GTSAC 2 cut(s) 530, 727
OliI CACNNNNGTG 1 cut(s) 1059
PaeR7I CTCGAG 1 cut(s) 429
PagI TCATGA 1 cut(s) 82
PcsI WCGNNNNNNNCGW 1 cut(s) 135
PdmI GAANNNNTTC 1 cut(s) 8
PfeI GAWTC 2 cut(s) 247, 601
PflFI GACNNNGTC 2 cut(s) 134, 926
PflMI CCANNNNNTGG 1 cut(s) 406
PkrI GCNGC 8 cut(s) 92, 457, 559, 589, 592, 937, 946, 988
PleI GAGTC 1 cut(s) 148
PpsI GAGTC 1 cut(s) 148
PsiI TTATAA 1 cut(s) 515
PspEI GGTNACC 1 cut(s) 530
PspN4I GGNNCC 1 cut(s) 261
PspPI GGNCC 1 cut(s) 736
PspXI VCTCGAGB 1 cut(s) 429
PstI CTGCAG 1 cut(s) 95
PsuI RGATCY 1 cut(s) 95
PsyI GACNNNGTC 2 cut(s) 134, 926
PvuII CAGCTG 2 cut(s) 458, 956
RsaI GTAC 5 cut(s) 163, 202, 483, 904, 1072
RsaNI GTAC 5 cut(s) 162, 201, 482, 903, 1071
RseI CAYNNNNRTG 2 cut(s) 314, 1059
SaqAI TTAA 1 cut(s) 321
SatI GCNGC 8 cut(s) 91, 456, 558, 588, 591, 936, 945, 987
Sau3AI GATC 3 cut(s) 95, 350, 499
Sau96I GGNCC 1 cut(s) 736
ScaI AGTACT 2 cut(s) 202, 904
SchI GAGTC 1 cut(s) 149
ScrFI CCNGG 1 cut(s) 467
SduI GDGCHC 3 cut(s) 55, 278, 349
SfaNI GCATC 4 cut(s) 368, 581, 602, 756
SfcI CTRYAG 2 cut(s) 91, 708
Sfr274I CTCGAG 1 cut(s) 429
SinI GGWCC 1 cut(s) 736
SlaI CTCGAG 1 cut(s) 429
SmiMI CAYNNNNRTG 2 cut(s) 314, 1059
SmlI CTYRAG 1 cut(s) 429
SmoI CTYRAG 1 cut(s) 429
SpeI ACTAGT 1 cut(s) 164
Sse9I AATT 6 cut(s) 32, 233, 799, 834, 866, 964
SsiI CCGC 1 cut(s) 494
SspMI CTAG 3 cut(s) 165, 279, 839
StyD4I CCNGG 1 cut(s) 465
StyI CCWWGG 3 cut(s) 278, 567, 732
TaaI ACNGT 3 cut(s) 75, 727, 1070
TaqI TCGA 5 cut(s) 124, 138, 343, 430, 502
TasI AATT 6 cut(s) 32, 233, 799, 834, 866, 964
TatI WGTACW 3 cut(s) 200, 902, 1070
TfiI GAWTC 2 cut(s) 247, 601
Tru1I TTAA 1 cut(s) 321
Tru9I TTAA 1 cut(s) 321
TscAI CASTG 2 cut(s) 80, 921
TseFI GTSAC 2 cut(s) 530, 727
TseI GCWGC 8 cut(s) 90, 455, 557, 587, 590, 935, 944, 986
Tsp45I GTSAC 2 cut(s) 530, 727
TspDTI ATGAA 3 cut(s) 17, 99, 246
TspRI CASTG 2 cut(s) 80, 921
Tth111I GACNNNGTC 2 cut(s) 134, 926
Van91I CCANNNNNTGG 1 cut(s) 406
VpaK11BI GGWCC 1 cut(s) 736
XapI RAATTY 4 cut(s) 233, 799, 834, 866
XcmI CCANNNNNNNNNTGG 1 cut(s) 107
XhoI CTCGAG 1 cut(s) 429
XmaJI CCTAGG 1 cut(s) 278
XmnI GAANNNNTTC 1 cut(s) 8
XspI CTAG 3 cut(s) 165, 279, 839
ZrmI AGTACT 2 cut(s) 202, 904
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.