Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000005

Orthogroup ID

OG0013643

Physical Location & Seq

Genomic Coordinates Forward (+)

18211532 .. 18215655

Gene Structure 4124 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug05G0187400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3789 bp

ATGAATATGGCTACTGCTGCTTCCTTTTGCTCTGCTATCATCCCTCGGGAGAAGTATGATGTATTTCTTAGTTTCAGAGGCGAGGATACCCGAAATACTTTTACCAGCCATTTTTATGAGGCTTTATGTCGGAACAAAATCCAAACTTACATAGACTACAAACTAGAGAGAGGAGATGAAATCTCACCTGCCCTTCTCAAAGCCATCAGCAAATCAAAGCTTTCGGTTATCATTTTCTCCAAAAACTATGCTTCTTCTACATGGTGCTTGGATGAACTTGTGCACATACTCCAGTGCAGAGGTGGACAGTTTGTTATACCCATCTTCTACGGCATAGATCCATCACATGTACGCAAACAATCAGGGTGTTATGAAGATGCATTTCTTGAACATGAGGAACGTTTCAAGGACTCGATGGACAAGGTGCGCATATGGAGAGTTGCTTTGACAACAGCAGCTAATATATCTGGATATGATTCCCAAACAATTAGGCTTGAGTCTGAATTAGTTAAGCTTGTGGTCAATGACATTTTGGCGAAATTGAATTGTAAATCACAAATCGCAAGTGGTTTAGAGGACCTGGTTGGAATCGAAAAACACATTCAACAAGTTGAATTGTTATTATGCCTCGACTCACTGGATGTCCAGATTGTAGGTATTTGGGGTATGGGTGGTATCGGTAAGACCACCATTGCTGATGTTGTATTTCACCGTCTCTCTTCTCAATTCGAAGCTTGCTGCTTTATTGCAAATGTCAGGGACGCATCAGAGACGAAACATGGACTAAATGATTTGCGAAATCAACTGTTTGGTACCATACTTAAGGAAGAAAATTTATATATGGCTGCTCCATCTATTGGATCAAGTTTTGCCAGAGAGAGGCTTCGCTGTACAAGGGCCCTCGTTGTTCTTGATGATGTGATTGATTTAGGCCAAGTAGAATATTTAACTGGGGGTTATCGTAATCTGTTTGGTCCTGGTAGTAGAATCATTATAACAAGTAGAAACAAGCGCACGTTTGGGAATGGTCTAGATGATGGGAAGATATATGAGGTTAAGAAATTAGATTATGATGAAGCTCTTGTGCTCTTTCATTTGAGAGCTAATATCTCCTTCCCAGGTGATTACACAACTTTTGCAAAACAGGTGGTAGATTATGCTGGGGGCAATCCATTGGCTCTTACAATGTTGGGCTCCTTATTCTCTCATTGCAGGAGCAAAGAAGACTGGGAAGGTGAATTAGATAAAGTGAAAAAATTTCCCAACCAAAATATCCAGAATGTATTGAGGTTGAGTTATGATGGATTACATCAAAATGAGAAGGATATATTTCTCGACATCGCATGTTTCTATAAAGGAGAGTCTATAGGTTATGCAAAAAGAATGTTAGATGCCTCTGGCTTCTGTGTGAATGCAGGAATAAGACTTCTCATTGATATGTCTCTTATATCAATAAACAGTAATTCTTCAAGACTAGAGATGCATGATTTGATACAAGAAATGGGTCAGACAATTGTTCTCGATGAATGCATTAAAGACCCTGGACAACGCAGTAGATTGTGGATTGCTAAGGATATCAGTTGTGTACTAAAACATAAGACGGGAACTCAAGCAATTGAATGCTTAGTCATGAACATGGACCACATTTCAGAACTGGACATAGAGCCTTCAGCCTTCAAAAAGATGCATTATCTAAGATTACTCAAGTTTAAGAGTTACTGGAACTCGAAATGTGTCAACCTTCCTCAAGGTCTCAAGTGTCTTCCTCAAGCCCTTAGAATTCTGCAGTGGGATGGCTACCCTTTGAATTCTCTGCCATCAAAGCTTTGTCCAGAAAATCTTGTTGAGCTTTGTATGCCCTTCAGTCGACTTGAGCGACTTTGGACTAAAGGCCTGAAGATCAATCTTGGGAGCTTAAAACGCATCAATCTTACTCAGTCAAAGCACCTGGCTGAGGTTCCAGATCTCTCTGAGAGTCCAAATCTTGAGAGAATTAATCTTTCGGGTTGTACAAGTTTGGTTCAACTGCCTTCATCTTTTCAGCATCTTGCCAAGCTTACGAGTCTCTATCTGGGTAGCTGCTCCAATCTCAAAGTTCTCCCAGAGATGCCTGGCAATCTGGAAGTCTTACAATTGAGTGAGACTGCAATAGAAGAATTGCCTTCATCAATTTGGTGTCTCGAGAAGCTTAATGTATTGGATCTTGGACTATGTGAAAAGCTTAAGACTCTTCCAAGCAGCACTTGTAGGCTGAATTCGCTCCAATCTCTTTATCTATATGGCTGCCTCTCTCTCGAGTCTTTGCCTGTCGAGCTCCCTTCTGGGCTGAAGAAGCTGCAAGTGAAAAATTGCAAGAGTCTTGGGTCTTTGCCTGTCGAGCTCCCTCCTGGGCTGCAGTACCTGGACTTGAGCAATTGCAAGAGGCTTGGGTCTTTGCCTGTCGAGCTCCCTTCTGGGCTGTTGGAATTGGATTTGAGAATTTGCAAGAGTCTTCGATCTTTGCCTGTCGAGCTCCCTTCTGGGCTGTTGGAATTGGAATTGAGAAATTGCCAGAGTCTTCGATCTTTGCCTGTCAAGCTCCCTTCTGGGCTGTTGGAATTGGATTTGGGAAATTGTGAGAGTCTTCGATCTTTGCCTGTCGAGCTCCCTTCTGGGCTGAAGAAGCTGACCTTGATCAATTGTGAGAGTCTTCGATCTCTACCTGTCAAGCTCCCTCCTGGGCTGTGGGAACTGGAATTGAGAAATTGTAAGAGGCTTGGGTCTTTGCCTGTCGAGCTCCCTCCTGGGCTGCAGTACCTGGACTTGAGCAATTGCAAGAGGCTTGGGTCTTTGCCTGTCGAGCTTCCTTATTGGCTGCAGTACCTGGACTTGGGCAATTGCAAGAGTCTTGGGTCTTTACCCAAGCTGCCATGGCCTCTAAAGAAGTTAGATGCACGTGGCTGCACGTCACTAGAGACAGTATCAAGTTCGATGAGGACTGCACCCGGGCAAGGTAATGAAGGTATCGATCAATATGTCGACAATGATGAAGACGTTGAGGAGGAACTTTTATTTATAAATTGCGTGAAATTGGATCAGAATGCACGGAGCAACATAATGGCTGATGCACAGCTTAGAGTTATGCGATATGCAACTGCATTCAAATTAGGGTGGGCGGGAGAAGTGATTAAAATTGTTTGTCCGGGAAATGAAATTCCAAAGTGGTTGAGGTATCAAAGTGAGGGATGTTCGATAAATATCAAGCTTCCTCCTCATTCGTTTCGTCAAGGCTTCCTCGGTTATGCTGTATGCGTTGTGGTTGCATTCAACAACTATACGCCACCACCCAATATTGGGAATTCCTACTTTCTTTGTCAAGCCCATCACAAAACTAACAATGGTCACAAAGATTGGTGCAAAACCCTCTATATAAGGCTACGAGATGATATAATTCCCAATTCAGATCATGTGTTCCTGTGGTATTTAGGCCCTCATCTTGTTCGATCAGAAAGTACAATCAATCGCAAGGATTGGGTAAAAGCATCCGAGGCCTCTTTTGACTTCATGTTGTACCACTCTACTTGTGCAAGTAGAAAAAAATATCCCTTAGACCTCTCCACTGTGAGGGTGAAAAGGTGTGGAGTCTCCTTGTTGTATGCCCAAGATTTTGAAAAATTAGTTGAAGGCATTGATGAAGATGTAGTTATGGAACCAAAACAAGTAGTTGAAAGCGGAGCTGCAATCTTGAAGGAAGAAGATGACGGCGAACTACTTCCCCAGAGTAGTAAAAGGAAAATCAACATGTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0006355 GO:0006950 GO:0007154 GO:0008150 GO:0009267 GO:0009605 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0009991 GO:0010033 GO:0010036 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016036 GO:0019219 GO:0019222 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031667 GO:0031668 GO:0031669 GO:0033554 GO:0042221 GO:0042493 GO:0042594 GO:0044212 GO:0045892 GO:0045934 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0071496 GO:0080029 GO:0080090 GO:0080169 GO:0097159 GO:0140110 GO:1901363 GO:1901698 GO:1901700 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WRKY

Protein Analysis

1262

Amino Acids

141.79

Weight (kDa)

6.77

Isoelectric Point (pI)

50.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TIR	PF01582	18 - 197	6.4e-54	TIR domain
TIR_2	PF13676	21 - 113	1.1e-11	TIR domain
NB-ARC	PF00931	198 - 367	5.8e-15	NB-ARC domain
WHD_ROQ1	PF23282	438 - 507	1e-20	Disease resistance protein Roq1-like, winged-helix domain
LRR_14	PF23598	707 - 780	5.7e-07	Leucine-rich repeat region
C-JID	PF20160	1060 - 1216	1.1e-17	C-JID domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0013643)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g23150
malus_domestica	MD03G1197600.v1.1 MD11G1213500.v1.1
prunus_persica	Prupe.4G217900_v2.0.a1
pyrus_communis	pycom03g14910 pycom11g18810 pycom12g06880
rosa_chinensis	RchiOBHm_Chr5g0040801
rosa_laevigata	RLG00000034002
rosa_multiflora	Rmu_sc0001579.1_g000002
rosa_roxburghii	Rroxscaffold_1G00039900
rosa_rugosa	Rorug05G0187400 Rorug05G0187500
rosa_samantha	Rh5AG273100 Rh5BG278200 Rh5CG310200 Rh5DG286100
rosa_wichuraiana	Rw5G025680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	995, 3056
AarI	CACCTGC	1 cut(s)	196
Acc16I	TGCGCA	1 cut(s)	428
Acc36I	ACCTGC	1 cut(s)	196
Acc65I	GGTACC	1 cut(s)	812
AccB1I	GGYRCC	1 cut(s)	812
AccB7I	CCANNNNNTGG	1 cut(s)	857
AccI	GTMKAC	2 cut(s)	1867, 3018
AciI	CCGC	2 cut(s)	3155, 3711
AclI	AACGTT	1 cut(s)	400
AclWI	GGATC	4 cut(s)	332, 868, 2208, 3081
AcsI	RAATTY	8 cut(s)	832, 1256, 1779, 1807, 2254, 2478, 3192, 3337
AcuI	CTGAAG	5 cut(s)	1653, 1846, 1916, 2348, 2678
AcvI	CACGTG	1 cut(s)	2936
AfiI	CCNNNNNNNGG	8 cut(s)	857, 879, 1954, 2869, 2990, 3332, 3333, 3603
AflII	CTTAAG	2 cut(s)	821, 2222
AflIII	ACRYGT	2 cut(s)	346, 3780
AjiI	CACGTC	1 cut(s)	2946
Alw21I	GWGCWC	8 cut(s)	285, 1089, 2316, 2382, 2448, 2514, 2646, 2778
Alw26I	GTCTC	9 cut(s)	719, 764, 1446, 1757, 2069, 2135, 2183, 2948, 3628
Alw44I	GTGCAC	1 cut(s)	281
AlwI	GGATC	4 cut(s)	332, 868, 2208, 3081
AlwNI	CAGNNNCTG	3 cut(s)	2401, 2797, 2863
Ama87I	CYCGRG	4 cut(s)	45, 2180, 2294, 2984
AoxI	GGCC	6 cut(s)	897, 931, 1891, 2912, 3466, 3528
ApaI	GGGCCC	1 cut(s)	901
ApaLI	GTGCAC	1 cut(s)	281
ApoI	RAATTY	8 cut(s)	832, 1256, 1779, 1807, 2254, 2478, 3192, 3337
AseI	ATTAAT	1 cut(s)	1995
Asp700I	GAANNNNTTC	1 cut(s)	3750
Asp718I	GGTACC	1 cut(s)	812
AspLEI	GCGC	2 cut(s)	429, 1014
AspS9I	GGNCC	6 cut(s)	577, 897, 898, 974, 1639, 3467
AsuC2I	CCSGG	3 cut(s)	2985, 2986, 3183
AsuHPI	GGTGA	5 cut(s)	177, 701, 1133, 1247, 3619
AsuII	TTCGAA	1 cut(s)	729
AvaI	CYCGRG	4 cut(s)	45, 2180, 2294, 2984
AvaII	GGWCC	3 cut(s)	577, 974, 1639
BaeGI	GKGCMC	2 cut(s)	285, 901
BanI	GGYRCC	1 cut(s)	812
BanII	GRGCYC	8 cut(s)	901, 1196, 2316, 2382, 2448, 2514, 2646, 2778
BbrPI	CACGTG	1 cut(s)	2936
BbsI	GAAGAC	7 cut(s)	1230, 1754, 2483, 2549, 2615, 2681, 3036
Bbv12I	GWGCWC	8 cut(s)	285, 1089, 2316, 2382, 2448, 2514, 2646, 2778
BbvCI	CCTCAGC	1 cut(s)	1953
BceAI	ACGGC	2 cut(s)	346, 3757
BciVI	GTATCC	1 cut(s)	79
BclI	TGATCA	1 cut(s)	2673
BcnI	CCSGG	3 cut(s)	2985, 2986, 3183
BcoDI	GTCTC	9 cut(s)	719, 764, 1446, 1757, 2069, 2135, 2183, 2948, 3628
BfaI	CTAG	4 cut(s)	164, 1031, 1475, 2951
BfmI	CTRYAG	5 cut(s)	1365, 1784, 2393, 2789, 2855
BfrI	CTTAAG	2 cut(s)	821, 2222
BfuAI	ACCTGC	1 cut(s)	196
BfuI	GTATCC	1 cut(s)	79
BglII	AGATCT	1 cut(s)	1963
Bme18I	GGWCC	3 cut(s)	577, 974, 1639
BmeT110I	CYCGRG	4 cut(s)	45, 2180, 2294, 2984
BmgBI	CACGTC	1 cut(s)	2946
BmgT120I	GGNCC	6 cut(s)	577, 897, 898, 974, 1639, 3467
BmiI	GGNNCC	5 cut(s)	814, 899, 1195, 1959, 3690
BmrI	ACTGGG	2 cut(s)	960, 1237
BmuI	ACTGGG	2 cut(s)	960, 1237
BpiI	GAAGAC	7 cut(s)	1230, 1754, 2483, 2549, 2615, 2681, 3036
BpmI	CTGGAG	1 cut(s)	275
Bpu10I	CCTNAGC	2 cut(s)	1569, 1953
Bpu14I	TTCGAA	1 cut(s)	729
BpuMI	CCSGG	3 cut(s)	2985, 2986, 3183
Bsa29I	ATCGAT	1 cut(s)	3006
BsaAI	YACGTR	1 cut(s)	2936
BsaBI	GATNNNNATC	1 cut(s)	3236
BsaI	GGTCTC	1 cut(s)	1757
Bsc4I	CCNNNNNNNGG	8 cut(s)	857, 879, 1954, 2869, 2990, 3332, 3333, 3603
Bse1I	ACTGG	7 cut(s)	292, 642, 955, 1232, 1659, 1724, 2736
Bse3DI	GCAATG	2 cut(s)	690, 1207
Bse8I	GATNNNNATC	1 cut(s)	3236
BseCI	ATCGAT	1 cut(s)	3006
BseGI	GGATG	6 cut(s)	39, 277, 646, 1798, 3230, 3521
BseJI	GATNNNNATC	1 cut(s)	3236
BseLI	CCNNNNNNNGG	8 cut(s)	857, 879, 1954, 2869, 2990, 3332, 3333, 3603
BseMI	GCAATG	2 cut(s)	690, 1207
BseMII	CTCAG	3 cut(s)	1944, 1949, 1962
BseNI	ACTGG	7 cut(s)	292, 642, 955, 1232, 1659, 1724, 2736
BseRI	GAGGAG	3 cut(s)	186, 3053, 3240
BseSI	GKGCMC	2 cut(s)	285, 901
BseYI	CCCAGC	1 cut(s)	1160
BsgI	GTGCAG	3 cut(s)	316, 2926, 2964
BshFI	GGCC	6 cut(s)	899, 933, 1893, 2914, 3468, 3530
BshNI	GGYRCC	1 cut(s)	812
BshVI	ATCGAT	1 cut(s)	3006
BsiHKAI	GWGCWC	8 cut(s)	285, 1089, 2316, 2382, 2448, 2514, 2646, 2778
BsiHKCI	CYCGRG	4 cut(s)	45, 2180, 2294, 2984
BsiSI	CCGG	2 cut(s)	2985, 3182
BslFI	GGGAC	1 cut(s)	773
BslI	CCNNNNNNNGG	8 cut(s)	857, 879, 1954, 2869, 2990, 3332, 3333, 3603
BsmAI	GTCTC	9 cut(s)	719, 764, 1446, 1757, 2069, 2135, 2183, 2948, 3628
BsmBI	CGTCTC	2 cut(s)	719, 764
BsmFI	GGGAC	1 cut(s)	773
BsmI	GAATGC	6 cut(s)	1417, 1532, 1625, 3085, 3137, 3302
BsnI	GGCC	6 cut(s)	899, 933, 1893, 2914, 3468, 3530
Bso31I	GGTCTC	1 cut(s)	1757
BsoBI	CYCGRG	4 cut(s)	45, 2180, 2294, 2984
Bsp119I	TTCGAA	1 cut(s)	729
Bsp120I	GGGCCC	1 cut(s)	897
Bsp1407I	TGTACA	2 cut(s)	890, 2009
Bsp19I	CCATGG	1 cut(s)	2909
BspACI	CCGC	2 cut(s)	3155, 3711
BspANI	GGCC	6 cut(s)	899, 933, 1893, 2914, 3468, 3530
BspCNI	CTCAG	3 cut(s)	1945, 1948, 1963
BspDI	ATCGAT	1 cut(s)	3006
BspHI	TCATGA	1 cut(s)	1629
BspLI	GGNNCC	5 cut(s)	814, 899, 1195, 1959, 3690
BspMAI	CTGCAG	4 cut(s)	1788, 2397, 2793, 2859
BspMI	ACCTGC	1 cut(s)	196
BspPI	GGATC	4 cut(s)	332, 868, 2208, 3081
BspT104I	TTCGAA	1 cut(s)	729
BspT107I	GGYRCC	1 cut(s)	812
BspTI	CTTAAG	2 cut(s)	821, 2222
BspTNI	GGTCTC	1 cut(s)	1757
BsrDI	GCAATG	2 cut(s)	690, 1207
BsrGI	TGTACA	2 cut(s)	890, 2009
BsrI	ACTGG	7 cut(s)	292, 642, 955, 1232, 1659, 1724, 2736
BssT1I	CCWWGG	1 cut(s)	2909
Bst4CI	ACNGT	6 cut(s)	309, 713, 807, 1460, 2959, 3601
Bst6I	CTCTTC	2 cut(s)	724, 2235
BstAFI	CTTAAG	2 cut(s)	821, 2222
BstAUI	TGTACA	2 cut(s)	890, 2009
BstBAI	YACGTR	1 cut(s)	2936
BstBI	TTCGAA	1 cut(s)	729
BstC8I	GCNNGC	1 cut(s)	736
BstDSI	CCRYGG	1 cut(s)	2909
BstENI	CCTNNNNNAGG	1 cut(s)	1952
BstF5I	GGATG	6 cut(s)	39, 277, 646, 1798, 3230, 3521
BstHHI	GCGC	2 cut(s)	429, 1014
BstMAI	GTCTC	9 cut(s)	719, 764, 1446, 1757, 2069, 2135, 2183, 2948, 3628
BstNSI	RCATGY	3 cut(s)	350, 1347, 3784
BstSFI	CTRYAG	5 cut(s)	1365, 1784, 2393, 2789, 2855
BstSLI	GKGCMC	2 cut(s)	285, 901
BstV2I	GAAGAC	7 cut(s)	1230, 1754, 2483, 2549, 2615, 2681, 3036
BstX2I	RGATCY	3 cut(s)	337, 1963, 2200
BstYI	RGATCY	3 cut(s)	337, 1963, 2200
Bsu15I	ATCGAT	1 cut(s)	3006
BsuI	GTATCC	1 cut(s)	79
BsuRI	GGCC	6 cut(s)	899, 933, 1893, 2914, 3468, 3530
BsuTUI	ATCGAT	1 cut(s)	3006
BtgI	CCRYGG	1 cut(s)	2909
BtgZI	GCGATG	1 cut(s)	1324
BtrI	CACGTC	1 cut(s)	2946
BtsCI	GGATG	6 cut(s)	39, 277, 646, 1798, 3230, 3521
BtsI	GCAGTG	1 cut(s)	1793
BtsIMutI	CAGTG	4 cut(s)	299, 635, 1793, 3597
BveI	ACCTGC	1 cut(s)	196
Cac8I	GCNNGC	1 cut(s)	736
CaiI	CAGNNNCTG	3 cut(s)	2401, 2797, 2863
CciI	TCATGA	1 cut(s)	1629
CfoI	GCGC	2 cut(s)	429, 1014
Cfr13I	GGNCC	6 cut(s)	577, 897, 898, 974, 1639, 3467
Cfr9I	CCCGGG	1 cut(s)	2984
ClaI	ATCGAT	1 cut(s)	3006
CseI	GACGC	1 cut(s)	770
CsiI	ACCWGGT	1 cut(s)	579
CspCI	CAANNNNNGTGG	4 cut(s)	1128, 1163, 3542, 3577
Eam1104I	CTCTTC	2 cut(s)	724, 2235
EarI	CTCTTC	2 cut(s)	724, 2235
Ecl136II	GAGCTC	6 cut(s)	2314, 2380, 2446, 2512, 2644, 2776
Eco130I	CCWWGG	1 cut(s)	2909
Eco147I	AGGCCT	2 cut(s)	1893, 3530
Eco24I	GRGCYC	8 cut(s)	901, 1196, 2316, 2382, 2448, 2514, 2646, 2778
Eco31I	GGTCTC	1 cut(s)	1757
Eco32I	GATATC	1 cut(s)	1576
Eco47I	GGWCC	3 cut(s)	577, 974, 1639
Eco53kI	GAGCTC	6 cut(s)	2314, 2380, 2446, 2512, 2644, 2776
Eco57I	CTGAAG	5 cut(s)	1653, 1846, 1916, 2348, 2678
Eco72I	CACGTG	1 cut(s)	2936
Eco88I	CYCGRG	4 cut(s)	45, 2180, 2294, 2984
EcoICRI	GAGCTC	6 cut(s)	2314, 2380, 2446, 2512, 2644, 2776
EcoNI	CCTNNNNNAGG	1 cut(s)	1952
EcoO109I	RGGNCCY	4 cut(s)	577, 897, 898, 3467
EcoRI	GAATTC	4 cut(s)	1779, 1807, 2254, 3337
EcoRV	GATATC	1 cut(s)	1576
EcoT14I	CCWWGG	1 cut(s)	2909
EcoT22I	ATGCAT	4 cut(s)	382, 1485, 1532, 1689
EcoT38I	GRGCYC	8 cut(s)	901, 1196, 2316, 2382, 2448, 2514, 2646, 2778
ErhI	CCWWGG	1 cut(s)	2909
Esp3I	CGTCTC	2 cut(s)	719, 764
FalI	AAGNNNNNCTT	4 cut(s)	2227, 2259, 2654, 2686
FaqI	GGGAC	1 cut(s)	773
FauI	CCCGC	1 cut(s)	3148
FauNDI	CATATG	1 cut(s)	431
FbaI	TGATCA	1 cut(s)	2673
FblI	GTMKAC	2 cut(s)	1867, 3018
FokI	GGATG	6 cut(s)	26, 284, 653, 1805, 3237, 3508
FriOI	GRGCYC	8 cut(s)	901, 1196, 2316, 2382, 2448, 2514, 2646, 2778
FspAI	RTGCGCAY	1 cut(s)	428
FspBI	CTAG	4 cut(s)	164, 1031, 1475, 2951
FspI	TGCGCA	1 cut(s)	428
GlaI	GCGC	2 cut(s)	428, 1013
GsaI	CCCAGC	1 cut(s)	1164
GsuI	CTGGAG	1 cut(s)	275
HaeIII	GGCC	6 cut(s)	899, 933, 1893, 2914, 3468, 3530
HapII	CCGG	2 cut(s)	2985, 3182
HgaI	GACGC	1 cut(s)	770
HhaI	GCGC	2 cut(s)	429, 1014
Hin6I	GCGC	2 cut(s)	427, 1012
HinP1I	GCGC	2 cut(s)	427, 1012
HincII	GTYRAC	3 cut(s)	1738, 1868, 3019
HindII	GTYRAC	3 cut(s)	1738, 1868, 3019
HindIII	AAGCTT	8 cut(s)	218, 512, 732, 1823, 2054, 2186, 2219, 3242
HpaII	CCGG	2 cut(s)	2985, 3182
HphI	GGTGA	5 cut(s)	177, 701, 1133, 1247, 3619
Hpy166II	GTNNAC	6 cut(s)	283, 305, 1586, 1738, 1868, 3019
Hpy8I	GTNNAC	6 cut(s)	283, 305, 1586, 1738, 1868, 3019
HpyCH4III	ACNGT	6 cut(s)	309, 713, 807, 1460, 2959, 3601
HpyCH4IV	ACGT	5 cut(s)	400, 1016, 2935, 2945, 3033
HpySE526I	ACGT	5 cut(s)	400, 1016, 2935, 2945, 3033
HspAI	GCGC	2 cut(s)	427, 1012
KpnI	GGTACC	1 cut(s)	816
Ksp22I	TGATCA	1 cut(s)	2673
MabI	ACCWGGT	1 cut(s)	579
MaeI	CTAG	4 cut(s)	164, 1031, 1475, 2951
MaeII	ACGT	5 cut(s)	400, 1016, 2935, 2945, 3033
MaeIII	GTNAC	3 cut(s)	1715, 2946, 3380
MfeI	CAATTG	7 cut(s)	1512, 1614, 2132, 2413, 2677, 2809, 2875
MflI	RGATCY	3 cut(s)	337, 1963, 2200
MmeI	TCCRAC	5 cut(s)	110, 565, 2442, 2508, 2574
Mph1103I	ATGCAT	4 cut(s)	382, 1485, 1532, 1689
MroXI	GAANNNNTTC	1 cut(s)	3750
MslI	CAYNNNNRTG	4 cut(s)	114, 1314, 1436, 1634
MspCI	CTTAAG	2 cut(s)	821, 2222
MspI	CCGG	2 cut(s)	2985, 3182
MunI	CAATTG	7 cut(s)	1512, 1614, 2132, 2413, 2677, 2809, 2875
Mva1269I	GAATGC	6 cut(s)	1417, 1532, 1625, 3085, 3137, 3302
NciI	CCSGG	3 cut(s)	2985, 2986, 3183
NcoI	CCATGG	1 cut(s)	2909
NdeI	CATATG	1 cut(s)	431
NlaIV	GGNNCC	5 cut(s)	814, 899, 1195, 1959, 3690
NmuCI	GTSAC	2 cut(s)	2946, 3380
NsbI	TGCGCA	1 cut(s)	428
NsiI	ATGCAT	4 cut(s)	382, 1485, 1532, 1689
NspI	RCATGY	3 cut(s)	350, 1347, 3784
NspV	TTCGAA	1 cut(s)	729
PaeR7I	CTCGAG	2 cut(s)	2180, 2294
PagI	TCATGA	1 cut(s)	1629
PaqCI	CACCTGC	1 cut(s)	196
PceI	AGGCCT	2 cut(s)	1893, 3530
PciI	ACATGT	2 cut(s)	346, 3780
PcsI	WCGNNNNNNNCGW	1 cut(s)	1873
PctI	GAATGC	6 cut(s)	1417, 1532, 1625, 3085, 3137, 3302
PdmI	GAANNNNTTC	1 cut(s)	3750
PfeI	GAWTC	3 cut(s)	476, 588, 987
PflMI	CCANNNNNTGG	1 cut(s)	857
PfoI	TCCNGGA	1 cut(s)	3181
PmaCI	CACGTG	1 cut(s)	2936
PmlI	CACGTG	1 cut(s)	2936
Ppu21I	YACGTR	1 cut(s)	2936
PpuMI	RGGWCCY	1 cut(s)	577
PscI	ACATGT	2 cut(s)	346, 3780
PshBI	ATTAAT	1 cut(s)	1995
PsiI	TTATAA	2 cut(s)	995, 3056
Psp124BI	GAGCTC	6 cut(s)	2316, 2382, 2448, 2514, 2646, 2778
Psp1406I	AACGTT	1 cut(s)	400
Psp5II	RGGWCCY	1 cut(s)	577
PspCI	CACGTG	1 cut(s)	2936
PspFI	CCCAGC	1 cut(s)	1160
PspN4I	GGNNCC	5 cut(s)	814, 899, 1195, 1959, 3690
PspOMI	GGGCCC	1 cut(s)	897
PspPI	GGNCC	6 cut(s)	577, 897, 898, 974, 1639, 3467
PspPPI	RGGWCCY	1 cut(s)	577
PstI	CTGCAG	4 cut(s)	1788, 2397, 2793, 2859
PstNI	CAGNNNCTG	3 cut(s)	2401, 2797, 2863
PsuI	RGATCY	3 cut(s)	337, 1963, 2200
RseI	CAYNNNNRTG	4 cut(s)	114, 1314, 1436, 1634
SacI	GAGCTC	6 cut(s)	2316, 2382, 2448, 2514, 2646, 2778
SalI	GTCGAC	2 cut(s)	1866, 3017
Sau96I	GGNCC	6 cut(s)	577, 897, 898, 974, 1639, 3467
SexAI	ACCWGGT	1 cut(s)	579
SfcI	CTRYAG	5 cut(s)	1365, 1784, 2393, 2789, 2855
Sfr274I	CTCGAG	2 cut(s)	2180, 2294
SfuI	TTCGAA	1 cut(s)	729
SinI	GGWCC	3 cut(s)	577, 974, 1639
SlaI	CTCGAG	2 cut(s)	2180, 2294
SmaI	CCCGGG	1 cut(s)	2986
SmiMI	CAYNNNNRTG	4 cut(s)	114, 1314, 1436, 1634
SseBI	AGGCCT	2 cut(s)	1893, 3530
SsiI	CCGC	2 cut(s)	3155, 3711
SspI	AATATT	2 cut(s)	944, 3331
SspMI	CTAG	4 cut(s)	164, 1031, 1475, 2951
SstI	GAGCTC	6 cut(s)	2316, 2382, 2448, 2514, 2646, 2778
StuI	AGGCCT	2 cut(s)	1893, 3530
StyI	CCWWGG	1 cut(s)	2909
TaaI	ACNGT	6 cut(s)	309, 713, 807, 1460, 2959, 3601
TaiI	ACGT	5 cut(s)	403, 1019, 2938, 2948, 3036
TatI	WGTACW	4 cut(s)	890, 1585, 2009, 3491
TfiI	GAWTC	3 cut(s)	476, 588, 987
TscAI	CASTG	4 cut(s)	299, 642, 1793, 3604
TseFI	GTSAC	2 cut(s)	2946, 3380
Tsp45I	GTSAC	2 cut(s)	2946, 3380
TspGWI	ACGGA	1 cut(s)	3100
TspMI	CCCGGG	1 cut(s)	2984
TspRI	CASTG	4 cut(s)	299, 642, 1793, 3604
Van91I	CCANNNNNTGG	1 cut(s)	857
Vha464I	CTTAAG	2 cut(s)	821, 2222
VneI	GTGCAC	1 cut(s)	281
VpaK11BI	GGWCC	3 cut(s)	577, 974, 1639
VspI	ATTAAT	1 cut(s)	1995
XagI	CCTNNNNNAGG	1 cut(s)	1952
XapI	RAATTY	8 cut(s)	832, 1256, 1779, 1807, 2254, 2478, 3192, 3337
XbaI	TCTAGA	1 cut(s)	1030
XceI	RCATGY	3 cut(s)	350, 1347, 3784
XcmI	CCANNNNNNNNNTGG	1 cut(s)	299
XhoI	CTCGAG	2 cut(s)	2180, 2294
XmaI	CCCGGG	1 cut(s)	2984
XmiI	GTMKAC	2 cut(s)	1867, 3018
XmnI	GAANNNNTTC	1 cut(s)	3750
XspI	CTAG	4 cut(s)	164, 1031, 1475, 2951
Zsp2I	ATGCAT	4 cut(s)	382, 1485, 1532, 1689

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.