Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0007763

Physical Location & Seq

Genomic Coordinates Reverse (-)

27208983 .. 27211702

Gene Structure 2720 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1198900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1482 bp

ATGCTTTTCCTGTGCTCTCCACGCCAACACATTACAGACTCTCTCTCTCTCTCTCTCTCTCTAAGCATTCCAATTTCTCTCATGGCTTTCGTAGCTCCGTCTCCTTCCACCCAATCATTGCTTCGCGCTTCGCTATCTTCCCACGAGCCCAAAGCTCAATGGACATCTTCTGTGAATCCGTCGGATCAATTTTCGTTTGCATCGACGGCTGTGGCCTCTTTCCAGAGACCATCATCGGCAAAATCCCTTTCCGCGGAATCGAAAAGGTGCAAATCAGTTGTTCCGCTTGCTGCAAAAGTGGCATCTCCAGATGTTGACAACAATGTGGTAGGAGAAGATTACGAAAAACTGGCAAAAGAGTTGGAAAACGCTTCCCCACTTCAAATCATGAAGAAGGCTCTTGACCAATTTGGGAATGACATTGCCATTGCTTTCAGCGGGGCTGAGGACGTCGCCTTGATCGAGTATGCCAAACTAACAGGTCGGCCCTTCAGGGTATTCAGCCTGGATACTGGGAGGCTTAACCCAGAGACGTACCAGTTTTTTGACACTGTTGAGAAGCACTATGGCATTCACATCGAATACATGTTTCCTGATGCTGTTGAAGTTCAAGCACTGGTGCGAGGCAAAGGACTGTTCTCATTCTATGAAGACGGCCACCAGGAGTGCTGCAGGGTAAGGAAGGTTAGGCCTTTAAGGCGAGCCCTTAAGGGTCTTCGTGCCTGGATCACTGGGCAGAGAAAAGACCAGTCTCCGGGCACAAGAGCTGAAATTCCGGTTGTCCAGGTGGACCCATCGTTTGAAGGAATTGATGGTGGAATTGGCAGCTTGGTGAAATGGAATCCTGTTGCTAACGTGGAAGGCCATGACATATGGAACTTCCTCCAAGTCATGAATGTACCTGTCAATCCTTTGCACTCGCAAGGATACATTTCAATCGGGTGTGAGCCATGCACAAGGCCGGTGCTGCCAGGGCAGCATGAAAGGGAGGGAAGATGGTGGTGGGAGGATGCTAAGGCAAAGGAATGTGGCCTTCATAACGGAAATATCAAAGAAAAAGAGGCGCTGCACCTCAATGGCAATGGGAATGGGATCTCAACAACAAATGGGAGTGCCAATGTCCCAGACATCTTTAACAGCAAGAACTTGGTTTCCTTGAGCAGGACTGGGATAGAGAACTTGGCTAGACTGGAAAACCGAAGTGAACCTTGGCTTGTTGTGCTTTATGCACCATGGTGCCGTTTCTGCCAGGCAATGGAAGGATCATATGTTGAATTAGCAGAGAATCTGGCAGGAACTGGGGTGAAGGTGGGTAAGTTCAGAGCTGATGGGGAACAGAAGAAATATGCGCAGCAAGAACTGAAGCTGGGGAGCTTCCCAACAATACTCTTCTTCCCCAAGCACTCGTCAAAGCCCGTCAAGTATCCATCAGAGAAGAGGGATGTAGCGTCGTTGTTGGCCTTTGTGAATGCTCTCCGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000103 GO:0003674 GO:0003824 GO:0004604 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006790 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009973 GO:0009987 GO:0016491 GO:0016667 GO:0016671 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044424 GO:0044444 GO:0044464 GO:0055114

KEGG Pathways

Metabolic & Signaling

ko00920 ko01120 map00920 map01120

Pfam Domains

Protein Families

PAPS_reduct Thioredoxin

Protein Analysis

494

Amino Acids

54.52

Weight (kDa)

8.26

Isoelectric Point (pI)

44.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PAPS_reduct	PF01507	141 - 321	3.9e-49	Phosphoadenosine phosphosulfate reductase domain
Thioredoxin	PF00085	397 - 489	6.5e-11	Thioredoxin

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007763)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G62180 AT1G62180 AT4G04610 AT4G21990 AT4G21990
fragaria_vesca	FvH4_3g01900
malus_domestica	MD03G1198900.v1.1 MD05G1348200.v1.1 MD10G1323000.v1.1 MD11G1215200.v1.1
prunus_persica	Prupe.4G019200_v2.0.a1 Prupe.4G216100_v2.0.a1
pyrus_communis	pycom03g15040 pycom05g31570 pycom10g27340
rosa_chinensis	RchiOBHm_Chr5g0002761
rosa_laevigata	RLG00000031056
rosa_multiflora	Rmu_sc0013603.1_g000020
rosa_roxburghii	Rroxscaffold_1G00073140
rosa_rugosa	Rorug04G0399100
rosa_samantha	Rh5AG022200 Rh5BG025100 Rh5DG023500
rosa_wichuraiana	Rw5G002070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	453
Acc16I	TGCGCA	1 cut(s)	1350
AccB1I	GGYRCC	1 cut(s)	1236
AccB7I	CCANNNNNTGG	1 cut(s)	1255
AccII	CGCG	2 cut(s)	126, 254
AciI	CCGC	4 cut(s)	252, 254, 284, 438
AclWI	GGATC	4 cut(s)	192, 734, 1100, 1270
AcoI	YGGCCR	1 cut(s)	655
AcsI	RAATTY	1 cut(s)	771
AcuI	CTGAAG	2 cut(s)	475, 1382
AcyI	GRCGYC	1 cut(s)	450
AfaI	GTAC	2 cut(s)	536, 900
AfiI	CCNNNNNNNGG	4 cut(s)	253, 754, 1161, 1255
AflII	CTTAAG	1 cut(s)	707
AflIII	ACRYGT	1 cut(s)	585
AgsI	TTSAA	6 cut(s)	383, 605, 611, 803, 936, 1274
AjnI	CCWGG	6 cut(s)	504, 660, 722, 783, 970, 1248
AjuI	GAANNNNNNNTTGG	4 cut(s)	105, 137, 1192, 1224
AleI	CACNNNNGTG	1 cut(s)	1234
AloI	GAACNNNNNNTCC	2 cut(s)	1136, 1168
AluBI	AGCT	7 cut(s)	95, 155, 767, 828, 1325, 1366, 1374
AluI	AGCT	7 cut(s)	95, 155, 767, 828, 1325, 1366, 1374
Alw21I	GWGCWC	1 cut(s)	17
Alw26I	GTCTC	4 cut(s)	105, 220, 524, 756
AlwI	GGATC	4 cut(s)	192, 734, 1100, 1270
AlwNI	CAGNNNCTG	1 cut(s)	1298
AoxI	GGCC	8 cut(s)	213, 485, 655, 689, 862, 959, 1030, 1458
ApeKI	GCWGC	7 cut(s)	290, 669, 825, 967, 976, 1066, 1351
ApoI	RAATTY	1 cut(s)	771
ArsI	GACNNNNNNTTYG	2 cut(s)	782, 814
AspLEI	GCGC	3 cut(s)	128, 1066, 1351
AspS9I	GGNCC	2 cut(s)	486, 790
AsuC2I	CCSGG	1 cut(s)	756
AsuHPI	GGTGA	2 cut(s)	844, 1315
AvaII	GGWCC	1 cut(s)	790
BaeGI	GKGCMC	1 cut(s)	761
BanI	GGYRCC	1 cut(s)	1236
BanII	GRGCYC	2 cut(s)	150, 706
BauI	CACGAG	1 cut(s)	143
BbsI	GAAGAC	2 cut(s)	657, 707
Bbv12I	GWGCWC	1 cut(s)	17
BbvCI	CCTCAGC	1 cut(s)	444
BbvI	GCAGC	7 cut(s)	277, 656, 837, 954, 988, 1053, 1363
BccI	CCATC	6 cut(s)	238, 802, 806, 990, 1322, 1435
BceAI	ACGGC	3 cut(s)	222, 670, 1224
BcgI	CGANNNNNNTGC	2 cut(s)	559, 593
BciT130I	CCWGG	6 cut(s)	506, 662, 724, 785, 972, 1250
BciVI	GTATCC	3 cut(s)	502, 920, 1434
BcnI	CCSGG	1 cut(s)	756
BcoDI	GTCTC	4 cut(s)	105, 220, 524, 756
BfaI	CTAG	1 cut(s)	1185
BfmI	CTRYAG	1 cut(s)	670
BfoI	RGCGCY	1 cut(s)	1067
BfrI	CTTAAG	1 cut(s)	707
BfuI	GTATCC	3 cut(s)	502, 920, 1434
BglI	GCCNNNNNGGC	1 cut(s)	697
BisI	GCNGC	7 cut(s)	291, 670, 826, 968, 977, 1067, 1352
BlsI	GCNGC	7 cut(s)	292, 671, 827, 969, 978, 1068, 1353
Bme1390I	CCNGG	7 cut(s)	506, 662, 724, 756, 785, 972, 1250
Bme18I	GGWCC	1 cut(s)	790
BmgT120I	GGNCC	2 cut(s)	486, 790
BmiI	GGNNCC	2 cut(s)	792, 1238
BmrFI	CCNGG	7 cut(s)	506, 662, 724, 756, 785, 972, 1250
BmrI	ACTGGG	4 cut(s)	522, 741, 1176, 1308
BmsI	GCATC	4 cut(s)	209, 311, 586, 1000
BmuI	ACTGGG	4 cut(s)	522, 741, 1176, 1308
BpiI	GAAGAC	2 cut(s)	657, 707
BpmI	CTGGAG	1 cut(s)	291
Bpu10I	CCTNAGC	2 cut(s)	444, 1014
BpuEI	CTTGAG	1 cut(s)	1177
BpuMI	CCSGG	1 cut(s)	756
BsaHI	GRCGYC	1 cut(s)	450
BsaI	GGTCTC	1 cut(s)	220
BsaJI	CCNNGG	4 cut(s)	252, 971, 1208, 1232
BsaWI	WCCGGW	2 cut(s)	775, 1476
Bsc4I	CCNNNNNNNGG	4 cut(s)	253, 754, 1161, 1255
Bse118I	RCCGGY	1 cut(s)	961
Bse1I	ACTGG	9 cut(s)	354, 517, 538, 621, 736, 748, 1171, 1194, 1303
Bse3DI	GCAATG	5 cut(s)	116, 420, 426, 1087, 1260
BseBI	CCWGG	6 cut(s)	506, 662, 724, 785, 972, 1250
BseDI	CCNNGG	4 cut(s)	252, 971, 1208, 1232
BseGI	GGATG	2 cut(s)	1015, 1447
BseLI	CCNNNNNNNGG	4 cut(s)	253, 754, 1161, 1255
BseMI	GCAATG	5 cut(s)	116, 420, 426, 1087, 1260
BseMII	CTCAG	1 cut(s)	435
BseNI	ACTGG	9 cut(s)	354, 517, 538, 621, 736, 748, 1171, 1194, 1303
BseSI	GKGCMC	1 cut(s)	761
BseXI	GCAGC	7 cut(s)	277, 656, 837, 954, 988, 1053, 1363
BseYI	CCCAGC	1 cut(s)	1366
BsgI	GTGCAG	1 cut(s)	1052
Bsh1236I	CGCG	2 cut(s)	126, 254
BshFI	GGCC	8 cut(s)	215, 487, 657, 691, 864, 961, 1032, 1460
BshNI	GGYRCC	1 cut(s)	1236
BsiHKAI	GWGCWC	1 cut(s)	17
BsiSI	CCGG	4 cut(s)	755, 776, 962, 1477
BslFI	GGGAC	1 cut(s)	1106
BslI	CCNNNNNNNGG	4 cut(s)	253, 754, 1161, 1255
BsmAI	GTCTC	4 cut(s)	105, 220, 524, 756
BsmBI	CGTCTC	2 cut(s)	105, 524
BsmFI	GGGAC	1 cut(s)	1106
BsmI	GAATGC	3 cut(s)	66, 570, 1474
BsnI	GGCC	8 cut(s)	215, 487, 657, 691, 864, 961, 1032, 1460
Bso31I	GGTCTC	1 cut(s)	220
Bsp1286I	GDGCHC	4 cut(s)	17, 150, 706, 761
Bsp143I	GATC	5 cut(s)	184, 459, 726, 1092, 1262
Bsp19I	CCATGG	1 cut(s)	1232
BspACI	CCGC	4 cut(s)	252, 254, 284, 438
BspANI	GGCC	8 cut(s)	215, 487, 657, 691, 864, 961, 1032, 1460
BspCNI	CTCAG	1 cut(s)	436
BspFNI	CGCG	2 cut(s)	126, 254
BspHI	TCATGA	2 cut(s)	387, 891
BspLI	GGNNCC	2 cut(s)	792, 1238
BspMAI	CTGCAG	1 cut(s)	674
BspPI	GGATC	4 cut(s)	192, 734, 1100, 1270
BspT107I	GGYRCC	1 cut(s)	1236
BspTI	CTTAAG	1 cut(s)	707
BspTNI	GGTCTC	1 cut(s)	220
BsrDI	GCAATG	5 cut(s)	116, 420, 426, 1087, 1260
BsrFI	RCCGGY	1 cut(s)	961
BsrI	ACTGG	9 cut(s)	354, 517, 538, 621, 736, 748, 1171, 1194, 1303
BssAI	RCCGGY	1 cut(s)	961
BssECI	CCNNGG	4 cut(s)	252, 971, 1208, 1232
BssMI	GATC	5 cut(s)	184, 459, 726, 1092, 1262
BssNI	GRCGYC	1 cut(s)	450
BssSI	CACGAG	1 cut(s)	143
BssT1I	CCWWGG	2 cut(s)	1208, 1232
Bst2BI	CACGAG	1 cut(s)	143
Bst2UI	CCWGG	6 cut(s)	506, 662, 724, 785, 972, 1250
Bst4CI	ACNGT	2 cut(s)	553, 636
Bst6I	CTCTTC	2 cut(s)	1394, 1430
BstACI	GRCGYC	1 cut(s)	450
BstAFI	CTTAAG	1 cut(s)	707
BstC8I	GCNNGC	2 cut(s)	288, 702
BstDEI	CTNAG	3 cut(s)	62, 444, 1014
BstDSI	CCRYGG	2 cut(s)	252, 1232
BstENI	CCTNNNNNAGG	1 cut(s)	1159
BstF5I	GGATG	2 cut(s)	1015, 1447
BstFNI	CGCG	2 cut(s)	126, 254
BstH2I	RGCGCY	1 cut(s)	1067
BstHHI	GCGC	3 cut(s)	128, 1066, 1351
BstKTI	GATC	5 cut(s)	187, 462, 729, 1095, 1265
BstMAI	GTCTC	4 cut(s)	105, 220, 524, 756
BstMBI	GATC	5 cut(s)	184, 459, 726, 1092, 1262
BstNI	CCWGG	6 cut(s)	506, 662, 724, 785, 972, 1250
BstNSI	RCATGY	1 cut(s)	589
BstSCI	CCNGG	7 cut(s)	504, 660, 722, 754, 783, 970, 1248
BstSFI	CTRYAG	1 cut(s)	670
BstSLI	GKGCMC	1 cut(s)	761
BstUI	CGCG	2 cut(s)	126, 254
BstV1I	GCAGC	7 cut(s)	277, 656, 837, 954, 988, 1053, 1363
BstV2I	GAAGAC	2 cut(s)	657, 707
BstX2I	RGATCY	1 cut(s)	1092
BstYI	RGATCY	1 cut(s)	1092
BsuI	GTATCC	3 cut(s)	502, 920, 1434
BsuRI	GGCC	8 cut(s)	215, 487, 657, 691, 864, 961, 1032, 1460
BtgI	CCRYGG	2 cut(s)	252, 1232
BtsCI	GGATG	2 cut(s)	1015, 1447
BtsIMutI	CAGTG	3 cut(s)	549, 614, 729
Cac8I	GCNNGC	2 cut(s)	288, 702
CaiI	CAGNNNCTG	1 cut(s)	1298
CciI	TCATGA	2 cut(s)	387, 891
CfoI	GCGC	3 cut(s)	128, 1066, 1351
Cfr10I	RCCGGY	1 cut(s)	961
Cfr13I	GGNCC	2 cut(s)	486, 790
Cfr42I	CCGCGG	1 cut(s)	255
CseI	GACGC	1 cut(s)	1437
Csp6I	GTAC	2 cut(s)	535, 899
CviAII	CATG	8 cut(s)	82, 388, 586, 866, 892, 951, 980, 1233
CviQI	GTAC	2 cut(s)	535, 899
DdeI	CTNAG	3 cut(s)	62, 444, 1014
DpnI	GATC	5 cut(s)	186, 461, 728, 1094, 1264
DpnII	GATC	5 cut(s)	184, 459, 726, 1092, 1262
EaeI	YGGCCR	1 cut(s)	655
Eam1104I	CTCTTC	2 cut(s)	1394, 1430
EarI	CTCTTC	2 cut(s)	1394, 1430
Eco130I	CCWWGG	2 cut(s)	1208, 1232
Eco147I	AGGCCT	1 cut(s)	691
Eco24I	GRGCYC	2 cut(s)	150, 706
Eco31I	GGTCTC	1 cut(s)	220
Eco47I	GGWCC	1 cut(s)	790
Eco57I	CTGAAG	2 cut(s)	475, 1382
EcoNI	CCTNNNNNAGG	1 cut(s)	1159
EcoRII	CCWGG	6 cut(s)	504, 660, 722, 783, 970, 1248
EcoT14I	CCWWGG	2 cut(s)	1208, 1232
EcoT38I	GRGCYC	2 cut(s)	150, 706
ErhI	CCWWGG	2 cut(s)	1208, 1232
Esp3I	CGTCTC	2 cut(s)	105, 524
FaeI	CATG	8 cut(s)	85, 391, 589, 869, 895, 954, 983, 1236
FalI	AAGNNNNNCTT	2 cut(s)	1192, 1224
FaqI	GGGAC	1 cut(s)	1106
FatI	CATG	8 cut(s)	81, 387, 585, 865, 891, 950, 979, 1232
FauI	CCCGC	1 cut(s)	431
FauNDI	CATATG	2 cut(s)	872, 1267
Fnu4HI	GCNGC	7 cut(s)	291, 670, 826, 968, 977, 1067, 1352
FokI	GGATG	2 cut(s)	1022, 1454
FriOI	GRGCYC	2 cut(s)	150, 706
Fsp4HI	GCNGC	7 cut(s)	291, 670, 826, 968, 977, 1067, 1352
FspBI	CTAG	1 cut(s)	1185
FspI	TGCGCA	1 cut(s)	1350
GlaI	GCGC	3 cut(s)	127, 1065, 1350
GluI	GCNGC	7 cut(s)	291, 670, 826, 968, 977, 1067, 1352
GsaI	CCCAGC	1 cut(s)	1370
GsuI	CTGGAG	1 cut(s)	291
HaeII	RGCGCY	1 cut(s)	1067
HaeIII	GGCC	8 cut(s)	215, 487, 657, 691, 864, 961, 1032, 1460
HapII	CCGG	4 cut(s)	755, 776, 962, 1477
HgaI	GACGC	1 cut(s)	1437
HhaI	GCGC	3 cut(s)	128, 1066, 1351
Hin1I	GRCGYC	1 cut(s)	450
Hin1II	CATG	8 cut(s)	85, 391, 589, 869, 895, 954, 983, 1236
Hin6I	GCGC	3 cut(s)	126, 1064, 1349
HinP1I	GCGC	3 cut(s)	126, 1064, 1349
HincII	GTYRAC	1 cut(s)	316
HindII	GTYRAC	1 cut(s)	316
HinfI	GANTC	5 cut(s)	38, 175, 257, 841, 1285
HpaII	CCGG	4 cut(s)	755, 776, 962, 1477
HphI	GGTGA	2 cut(s)	844, 1315
Hpy166II	GTNNAC	3 cut(s)	316, 790, 1205
Hpy188I	TCNGA	3 cut(s)	184, 1322, 1432
Hpy188III	TCNNGA	6 cut(s)	223, 308, 388, 401, 593, 892
Hpy8I	GTNNAC	3 cut(s)	316, 790, 1205
Hpy99I	CGWCG	4 cut(s)	184, 208, 455, 1453
HpyAV	CCTTC	9 cut(s)	114, 388, 499, 676, 797, 854, 1043, 1253, 1300
HpyCH4III	ACNGT	2 cut(s)	553, 636
HpyCH4IV	ACGT	3 cut(s)	450, 533, 855
HpyCH4V	TGCA	8 cut(s)	200, 270, 293, 672, 916, 954, 1069, 1229
HpyF3I	CTNAG	3 cut(s)	62, 444, 1014
HpySE526I	ACGT	3 cut(s)	450, 533, 855
Hsp92I	GRCGYC	1 cut(s)	450
Hsp92II	CATG	8 cut(s)	85, 391, 589, 869, 895, 954, 983, 1236
HspAI	GCGC	3 cut(s)	126, 1064, 1349
KspI	CCGCGG	1 cut(s)	255
Kzo9I	GATC	5 cut(s)	184, 459, 726, 1092, 1262
LmnI	GCTCC	2 cut(s)	100, 1371
Lsp1109I	GCAGC	7 cut(s)	277, 656, 837, 954, 988, 1053, 1363
LweI	GCATC	4 cut(s)	209, 311, 586, 1000
MaeI	CTAG	1 cut(s)	1185
MaeII	ACGT	3 cut(s)	450, 533, 855
MalI	GATC	5 cut(s)	186, 461, 728, 1094, 1264
MboI	GATC	5 cut(s)	184, 459, 726, 1092, 1262
MflI	RGATCY	1 cut(s)	1092
MhlI	GDGCHC	4 cut(s)	17, 150, 706, 761
MluCI	AATT	7 cut(s)	72, 188, 407, 771, 807, 819, 1274
MlyI	GAGTC	1 cut(s)	32
MmeI	TCCRAC	2 cut(s)	162, 342
MseI	TTAA	4 cut(s)	522, 695, 708, 1134
MslI	CAYNNNNRTG	2 cut(s)	1074, 1234
MspA1I	CMGCKG	2 cut(s)	254, 438
MspCI	CTTAAG	1 cut(s)	707
MspI	CCGG	4 cut(s)	755, 776, 962, 1477
MspR9I	CCNGG	7 cut(s)	506, 662, 724, 756, 785, 972, 1250
Mva1269I	GAATGC	3 cut(s)	66, 570, 1474
MvaI	CCWGG	6 cut(s)	506, 662, 724, 785, 972, 1250
MvnI	CGCG	2 cut(s)	126, 254
NciI	CCSGG	1 cut(s)	756
NcoI	CCATGG	1 cut(s)	1232
NdeI	CATATG	2 cut(s)	872, 1267
NdeII	GATC	5 cut(s)	184, 459, 726, 1092, 1262
NlaIII	CATG	8 cut(s)	85, 391, 589, 869, 895, 954, 983, 1236
NlaIV	GGNNCC	2 cut(s)	792, 1238
NsbI	TGCGCA	1 cut(s)	1350
NspI	RCATGY	1 cut(s)	589
OliI	CACNNNNGTG	1 cut(s)	1234
PagI	TCATGA	2 cut(s)	387, 891
PceI	AGGCCT	1 cut(s)	691
PciI	ACATGT	1 cut(s)	585
PcsI	WCGNNNNNNNCGW	2 cut(s)	200, 459
PctI	GAATGC	3 cut(s)	66, 570, 1474
PfeI	GAWTC	4 cut(s)	175, 257, 841, 1285
PflMI	CCANNNNNTGG	1 cut(s)	1255
PkrI	GCNGC	7 cut(s)	292, 671, 827, 969, 978, 1068, 1353
PleI	GAGTC	1 cut(s)	32
PpsI	GAGTC	1 cut(s)	32
PscI	ACATGT	1 cut(s)	585
Psp6I	CCWGG	6 cut(s)	504, 660, 722, 783, 970, 1248
PspFI	CCCAGC	1 cut(s)	1366
PspGI	CCWGG	6 cut(s)	504, 660, 722, 783, 970, 1248
PspN4I	GGNNCC	2 cut(s)	792, 1238
PspPI	GGNCC	2 cut(s)	486, 790
PstI	CTGCAG	1 cut(s)	674
PstNI	CAGNNNCTG	1 cut(s)	1298
PsuI	RGATCY	1 cut(s)	1092
RsaI	GTAC	2 cut(s)	536, 900
RsaNI	GTAC	2 cut(s)	535, 899
RseI	CAYNNNNRTG	2 cut(s)	1074, 1234
SacII	CCGCGG	1 cut(s)	255
SaqAI	TTAA	4 cut(s)	522, 695, 708, 1134
SatI	GCNGC	7 cut(s)	291, 670, 826, 968, 977, 1067, 1352
Sau3AI	GATC	5 cut(s)	184, 459, 726, 1092, 1262
Sau96I	GGNCC	2 cut(s)	486, 790
SchI	GAGTC	1 cut(s)	32
ScrFI	CCNGG	7 cut(s)	506, 662, 724, 756, 785, 972, 1250
SduI	GDGCHC	4 cut(s)	17, 150, 706, 761
SfaNI	GCATC	4 cut(s)	209, 311, 586, 1000
SfcI	CTRYAG	1 cut(s)	670
Sfr303I	CCGCGG	1 cut(s)	255
SgrBI	CCGCGG	1 cut(s)	255
SinI	GGWCC	1 cut(s)	790
SmiMI	CAYNNNNRTG	2 cut(s)	1074, 1234
SmlI	CTYRAG	2 cut(s)	707, 1156
SmoI	CTYRAG	2 cut(s)	707, 1156
Sse9I	AATT	7 cut(s)	72, 188, 407, 771, 807, 819, 1274
SseBI	AGGCCT	1 cut(s)	691
SsiI	CCGC	4 cut(s)	252, 254, 284, 438
SspMI	CTAG	1 cut(s)	1185
StuI	AGGCCT	1 cut(s)	691
StyD4I	CCNGG	7 cut(s)	504, 660, 722, 754, 783, 970, 1248
StyI	CCWWGG	2 cut(s)	1208, 1232
TaaI	ACNGT	2 cut(s)	553, 636
TaiI	ACGT	3 cut(s)	453, 536, 858
TaqI	TCGA	4 cut(s)	203, 260, 462, 579
TasI	AATT	7 cut(s)	72, 188, 407, 771, 807, 819, 1274
TfiI	GAWTC	4 cut(s)	175, 257, 841, 1285
Tru1I	TTAA	4 cut(s)	522, 695, 708, 1134
Tru9I	TTAA	4 cut(s)	522, 695, 708, 1134
TscAI	CASTG	3 cut(s)	556, 621, 736
TseI	GCWGC	7 cut(s)	290, 669, 825, 967, 976, 1066, 1351
TspDTI	ATGAA	5 cut(s)	404, 663, 908, 996, 1025
TspGWI	ACGGA	3 cut(s)	87, 168, 1056
TspRI	CASTG	3 cut(s)	556, 621, 736
Van91I	CCANNNNNTGG	1 cut(s)	1255
Vha464I	CTTAAG	1 cut(s)	707
VpaK11BI	GGWCC	1 cut(s)	790
XagI	CCTNNNNNAGG	1 cut(s)	1159
XapI	RAATTY	1 cut(s)	771
XceI	RCATGY	1 cut(s)	589
XspI	CTAG	1 cut(s)	1185
ZraI	GACGTC	1 cut(s)	451

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.