MD03G1212200.v1.1

Autophagy-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0006822
Physical Location & Seq
Forward (+)
29092442 .. 29095650
3209 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1212200.v1.1.491

Sequence Viewer

Length: 1584 bp
ATGGTCATTGTCAGAGATATTGTCAGTAAGGCTGTTATTGTGCAGTTCAGGGCGCACACGAGTCCTATTTCTGCGATACGCTTTGATCCTAGTGGCACCCTTTTAGTGACTGCATCAACTCAGGGGCATAACATCAATGTTTTTAAGATAATGCCTGGGAGCTCTCCTTCAACTGATGGTGGTGCATCATATGTACATCTTTACAGGCTGCAACGTGGATTAACAAATGCAATCATACAGGACATCAGTTTTAGTGATGACAGCAACTGGATCATGGTTAGTTCCTCTAGGGGGACAAGCCATCTGTTTGCAATAAATCCTTGGGGAGGATCAGTAAATCTCCCAACTGCCAATGCTGGTTTTACTACCAAAAGTACAGGATTGGGGGTTACAACTAGATCGTCAGTTCGTTGGCCAGGTTTGCAAACGCCTAATCAGCAAAGCCTTTGCTCAGCTGGTCCCCCGGTTACCCTCTCTGTTGTTGGTAGAATAAGAAATGGAAATAATAGTTGGAGAGGCACTGTAACTGGTGCTGCAGCCGCTGCAACAGGCAAGATGAGTTCCCTTTCTGGGGCAGTTGCTTCTTCATTCCACAATTGCAAAGGAAAAGCTTATTATGTGGATCGCAACTCTTCGAAGGCTAAGTACCATCTCTTGGTTTTTTCCCCTTCTGGTTCCATGATACAATATGCATTGCGAATATCAAATGGTCTAGATTTGACAGCTGTGGCTGGATTGAACAGTGCTTATGAGTCAGGCCTGGAGGGTGATACAAGATTAGTAGTTGAAGCTATCCGGAAGTGGAATGTATGTCAAAAGCAAAACCGAAGGGAACGTGATGATACTTCTGACATATATGGTGAGAATGTAAATTTCGACAACAATAAGATATATCCCGAAGGGAAGAAGAAGGGAAACACCATTTACCCAGAAGCTTGCATTACTCTCCCAAAAGCAAAGATCAGTTCTGAGGAAGAACATCAGTTGTATATTTCAGAAGCCGAACTGCAGATGCATGAAGCACGGAGTCCAGTATGGGCAAAACCTGAGCTATACTTTCAGTCAATGATCATGGAAGGTGTCAAAATGGATGATGAAACCACTTCAGGAGGGGAAATTGAGATCGAGAGGATTCCTACTCGCATGATTGAAGCAAGATCAAAGGACTTGGTACCAGTTTTTGAATATCTTCAAACTCCCATATTTCAACAAACAAGGGTTGCTTCTGTAGATAGCAATTTTAGCCGGCAACTTTCGCGTCAGAGGTCTGGTATATCTGAAAATGGTGGGCGTTCATGCAGAAACAGTTCAAGCTCTCTTGACACCATGACTGAAAGTGGTGCTGGAGTGGCAGAACTTACTAATGGGATTGGAGTAACTGGACGGGGTGGTTCTCAGATGCCCACCGAAAGCATGGGCTTTGTAAATAACAATGACAGCCCAAAGACAGAGACTCAGCTTGAGACTGTAAATAATAGAGAGAGCAGCTTAAAATTGGAGGCCCAACTTAAATCTGTAAATAATGACATAGACGGCCTGGGAGTGGGGAATCTGTTTGGAATCGAAGGTGATGAGTTTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000226 GO:0000785 GO:0001952 GO:0001953 GO:0003674 GO:0003682 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005730 GO:0005737 GO:0005856 GO:0005874 GO:0005881 GO:0006355 GO:0006357 GO:0006810 GO:0006950 GO:0006996 GO:0007010 GO:0007017 GO:0007030 GO:0007162 GO:0007275 GO:0008047 GO:0008092 GO:0008134 GO:0008150 GO:0009605 GO:0009653 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0009991 GO:0010256 GO:0010468 GO:0010556 GO:0010557 GO:0010594 GO:0010595 GO:0010604 GO:0010628 GO:0010632 GO:0010634 GO:0010638 GO:0010698 GO:0010810 GO:0010812 GO:0015630 GO:0015631 GO:0016043 GO:0016192 GO:0019219 GO:0019222 GO:0019899 GO:0030155 GO:0030234 GO:0030334 GO:0030335 GO:0031023 GO:0031252 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031344 GO:0031346 GO:0031667 GO:0031974 GO:0031981 GO:0032386 GO:0032388 GO:0032501 GO:0032502 GO:0032878 GO:0032879 GO:0032880 GO:0032956 GO:0032970 GO:0033043 GO:0033157 GO:0034260 GO:0035035 GO:0035148 GO:0035239 GO:0035257 GO:0035295 GO:0035327 GO:0040012 GO:0040017 GO:0042221 GO:0042393 GO:0042594 GO:0043085 GO:0043086 GO:0043087 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043547 GO:0043627 GO:0044087 GO:0044089 GO:0044092 GO:0044093 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0045111 GO:0045893 GO:0045935 GO:0045944 GO:0048487 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048646 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051049 GO:0051050 GO:0051128 GO:0051129 GO:0051130 GO:0051171 GO:0051173 GO:0051179 GO:0051222 GO:0051223 GO:0051234 GO:0051239 GO:0051240 GO:0051252 GO:0051254 GO:0051270 GO:0051272 GO:0051336 GO:0051345 GO:0051346 GO:0051427 GO:0051489 GO:0051491 GO:0051493 GO:0051495 GO:0051716 GO:0051893 GO:0051895 GO:0060255 GO:0060341 GO:0060491 GO:0065007 GO:0065009 GO:0070013 GO:0070201 GO:0070887 GO:0071391 GO:0071840 GO:0080090 GO:0090087 GO:0090109 GO:0090316 GO:0090630 GO:0098772 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0120032 GO:0120034 GO:0120035 GO:1901888 GO:1901889 GO:1902680 GO:1903391 GO:1903392 GO:1903506 GO:1903508 GO:1903827 GO:1903829 GO:1904951 GO:2000112 GO:2000114 GO:2000145 GO:2000147 GO:2000249 GO:2000251 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BCAS3 WD40

Protein Analysis

528

Amino Acids

57.13

Weight (kDa)

6.49

Isoelectric Point (pI)

46.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BCAS3_WD40 PF21034 3 - 113 2.9e-25 BCAS3, WD40 repeat
PROPPIN PF21032 5 - 107 6.9e-06 PROPPIN
BCAS3 PF12490 250 - 395 1.1e-52 BCAS3 microtubule associated cell migration factor, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1171
AccB1I GGYRCC 2 cut(s) 95, 1171
AccB7I CCANNNNNTGG 1 cut(s) 655
AccII CGCG 1 cut(s) 1258
AccIII TCCGGA 1 cut(s) 795
AciI CCGC 1 cut(s) 540
AclWI GGATC 4 cut(s) 80, 278, 337, 630
AcoI YGGCCR 1 cut(s) 413
AcsI RAATTY 1 cut(s) 871
AcuI CTGAAG 1 cut(s) 1089
AfaI GTAC 4 cut(s) 195, 376, 647, 1173
AfiI CCNNNNNNNGG 6 cut(s) 291, 326, 570, 571, 655, 1543
AgsI TTSAA 8 cut(s) 171, 739, 788, 1151, 1184, 1193, 1208, 1311
AjnI CCWGG 4 cut(s) 154, 415, 759, 1536
AjuI GAANNNNNNNTTGG 2 cut(s) 493, 525
Alw21I GWGCWC 1 cut(s) 164
Alw26I GTCTC 2 cut(s) 1445, 1457
AlwI GGATC 4 cut(s) 80, 278, 337, 630
AlwNI CAGNNNCTG 2 cut(s) 267, 542
Aor13HI TCCGGA 1 cut(s) 795
AoxI GGCC 4 cut(s) 413, 757, 1500, 1534
ApeKI GCWGC 5 cut(s) 208, 533, 536, 542, 1485
ApoI RAATTY 1 cut(s) 871
Asp700I GAANNNNTTC 2 cut(s) 1188, 1306
Asp718I GGTACC 1 cut(s) 1171
AspLEI GCGC 1 cut(s) 55
AspS9I GGNCC 2 cut(s) 458, 1501
AsuC2I CCSGG 1 cut(s) 464
AsuHPI GGTGA 3 cut(s) 779, 872, 1580
AsuII TTCGAA 1 cut(s) 635
AvaII GGWCC 1 cut(s) 458
BalI TGGCCA 1 cut(s) 415
BanI GGYRCC 2 cut(s) 95, 1171
BanII GRGCYC 1 cut(s) 164
BarI GAAGNNNNNNTAC 2 cut(s) 629, 661
BauI CACGAG 1 cut(s) 58
Bbv12I GWGCWC 1 cut(s) 164
BbvI GCAGC 5 cut(s) 195, 520, 529, 548, 1497
BccI CCATC 3 cut(s) 170, 309, 657
BceAI ACGGC 1 cut(s) 1549
BciT130I CCWGG 4 cut(s) 156, 417, 761, 1538
BclI TGATCA 1 cut(s) 1068
BcnI CCSGG 1 cut(s) 464
BcoDI GTCTC 2 cut(s) 1445, 1457
BfaI CTAG 4 cut(s) 90, 288, 396, 713
BfmI CTRYAG 3 cut(s) 534, 1007, 1227
BisI GCNGC 6 cut(s) 209, 534, 537, 540, 543, 1486
BlpI GCTNAGC 1 cut(s) 451
BlsI GCNGC 6 cut(s) 210, 535, 538, 541, 544, 1487
Bme1390I CCNGG 5 cut(s) 156, 417, 464, 761, 1538
Bme18I GGWCC 1 cut(s) 458
BmgT120I GGNCC 2 cut(s) 458, 1501
BmiI GGNNCC 4 cut(s) 97, 460, 676, 1173
BmrFI CCNGG 5 cut(s) 156, 417, 464, 761, 1538
BmsI GCATC 4 cut(s) 122, 194, 1002, 1389
BpmI CTGGAG 2 cut(s) 782, 1365
Bpu10I CCTNAGC 1 cut(s) 1047
Bpu1102I GCTNAGC 1 cut(s) 451
Bpu14I TTCGAA 1 cut(s) 635
BpuEI CTTGAG 1 cut(s) 1481
BpuMI CCSGG 1 cut(s) 464
BsaJI CCNNGG 4 cut(s) 155, 320, 462, 1537
BsaWI WCCGGW 1 cut(s) 795
BsaXI ACNNNNNCTCC 2 cut(s) 318, 348
Bsc4I CCNNNNNNNGG 6 cut(s) 291, 326, 570, 571, 655, 1543
Bse118I RCCGGY 1 cut(s) 1245
Bse1I ACTGG 5 cut(s) 272, 532, 1031, 1175, 1384
Bse3DI GCAATG 1 cut(s) 692
BseAI TCCGGA 1 cut(s) 795
BseBI CCWGG 4 cut(s) 156, 417, 761, 1538
BseDI CCNNGG 4 cut(s) 155, 320, 462, 1537
BseGI GGATG 1 cut(s) 1096
BseLI CCNNNNNNNGG 6 cut(s) 291, 326, 570, 571, 655, 1543
BseMI GCAATG 1 cut(s) 692
BseMII CTCAG 6 cut(s) 134, 465, 960, 1038, 1409, 1469
BseNI ACTGG 5 cut(s) 272, 532, 1031, 1175, 1384
BseXI GCAGC 5 cut(s) 195, 520, 529, 548, 1497
BsgI GTGCAG 1 cut(s) 62
Bsh1236I CGCG 1 cut(s) 1258
BshFI GGCC 4 cut(s) 415, 759, 1502, 1536
BshNI GGYRCC 2 cut(s) 95, 1171
BsiHKAI GWGCWC 1 cut(s) 164
BsiSI CCGG 3 cut(s) 464, 796, 1246
BslFI GGGAC 2 cut(s) 307, 444
BslI CCNNNNNNNGG 6 cut(s) 291, 326, 570, 571, 655, 1543
BsmAI GTCTC 2 cut(s) 1445, 1457
BsmFI GGGAC 2 cut(s) 307, 444
BsnI GGCC 4 cut(s) 415, 759, 1502, 1536
Bsp119I TTCGAA 1 cut(s) 635
Bsp1286I GDGCHC 1 cut(s) 164
Bsp13I TCCGGA 1 cut(s) 795
Bsp1407I TGTACA 1 cut(s) 193
Bsp143I GATC 9 cut(s) 85, 270, 329, 398, 622, 960, 1068, 1122, 1157
Bsp1720I GCTNAGC 1 cut(s) 451
BspACI CCGC 1 cut(s) 540
BspANI GGCC 4 cut(s) 415, 759, 1502, 1536
BspCNI CTCAG 6 cut(s) 133, 464, 961, 1039, 1408, 1468
BspEI TCCGGA 1 cut(s) 795
BspFNI CGCG 1 cut(s) 1258
BspLI GGNNCC 4 cut(s) 97, 460, 676, 1173
BspMAI CTGCAG 2 cut(s) 538, 1011
BspPI GGATC 4 cut(s) 80, 278, 337, 630
BspT104I TTCGAA 1 cut(s) 635
BspT107I GGYRCC 2 cut(s) 95, 1171
BsrDI GCAATG 1 cut(s) 692
BsrFI RCCGGY 1 cut(s) 1245
BsrGI TGTACA 1 cut(s) 193
BsrI ACTGG 5 cut(s) 272, 532, 1031, 1175, 1384
BssAI RCCGGY 1 cut(s) 1245
BssECI CCNNGG 4 cut(s) 155, 320, 462, 1537
BssMI GATC 9 cut(s) 85, 270, 329, 398, 622, 960, 1068, 1122, 1157
BssSI CACGAG 1 cut(s) 58
BssT1I CCWWGG 1 cut(s) 320
Bst2BI CACGAG 1 cut(s) 58
Bst2UI CCWGG 4 cut(s) 156, 417, 761, 1538
Bst4CI ACNGT 4 cut(s) 523, 743, 1307, 1468
Bst6I CTCTTC 1 cut(s) 637
BstAPI GCANNNNNTGC 1 cut(s) 542
BstAUI TGTACA 1 cut(s) 193
BstBI TTCGAA 1 cut(s) 635
BstC8I GCNNGC 2 cut(s) 937, 1247
BstDEI CTNAG 7 cut(s) 120, 451, 642, 969, 1047, 1395, 1455
BstEII GGTNACC 1 cut(s) 466
BstENI CCTNNNNNAGG 1 cut(s) 324
BstF5I GGATG 1 cut(s) 1096
BstFNI CGCG 1 cut(s) 1258
BstHHI GCGC 1 cut(s) 55
BstKTI GATC 9 cut(s) 88, 273, 332, 401, 625, 963, 1071, 1125, 1160
BstMAI GTCTC 2 cut(s) 1445, 1457
BstMBI GATC 9 cut(s) 85, 270, 329, 398, 622, 960, 1068, 1122, 1157
BstMWI GCNNNNNNNGC 7 cut(s) 421, 436, 539, 542, 1242, 1255, 1349
BstNI CCWGG 4 cut(s) 156, 417, 761, 1538
BstPI GGTNACC 1 cut(s) 466
BstSCI CCNGG 5 cut(s) 154, 415, 462, 759, 1536
BstSFI CTRYAG 3 cut(s) 534, 1007, 1227
BstUI CGCG 1 cut(s) 1258
BstV1I GCAGC 5 cut(s) 195, 520, 529, 548, 1497
BsuRI GGCC 4 cut(s) 415, 759, 1502, 1536
BtsCI GGATG 1 cut(s) 1096
BtsIMutI CAGTG 2 cut(s) 519, 748
Cac8I GCNNGC 2 cut(s) 937, 1247
CaiI CAGNNNCTG 2 cut(s) 267, 542
CfoI GCGC 1 cut(s) 55
Cfr10I RCCGGY 1 cut(s) 1245
Cfr13I GGNCC 2 cut(s) 458, 1501
CseI GACGC 1 cut(s) 1247
Csp6I GTAC 4 cut(s) 194, 375, 646, 1172
CviAII CATG 8 cut(s) 274, 679, 1016, 1072, 1144, 1296, 1327, 1414
CviQI GTAC 4 cut(s) 194, 375, 646, 1172
DdeI CTNAG 7 cut(s) 120, 451, 642, 969, 1047, 1395, 1455
DpnI GATC 9 cut(s) 87, 272, 331, 400, 624, 962, 1070, 1124, 1159
DpnII GATC 9 cut(s) 85, 270, 329, 398, 622, 960, 1068, 1122, 1157
EaeI YGGCCR 1 cut(s) 413
Eam1104I CTCTTC 1 cut(s) 637
EarI CTCTTC 1 cut(s) 637
Ecl136II GAGCTC 1 cut(s) 162
Eco130I CCWWGG 1 cut(s) 320
Eco147I AGGCCT 1 cut(s) 759
Eco24I GRGCYC 1 cut(s) 164
Eco47I GGWCC 1 cut(s) 458
Eco53kI GAGCTC 1 cut(s) 162
Eco57I CTGAAG 1 cut(s) 1089
Eco91I GGTNACC 1 cut(s) 466
EcoICRI GAGCTC 1 cut(s) 162
EcoNI CCTNNNNNAGG 1 cut(s) 324
EcoO65I GGTNACC 1 cut(s) 466
EcoRII CCWGG 4 cut(s) 154, 415, 759, 1536
EcoT14I CCWWGG 1 cut(s) 320
EcoT22I ATGCAT 2 cut(s) 694, 1017
EcoT38I GRGCYC 1 cut(s) 164
ErhI CCWWGG 1 cut(s) 320
FaeI CATG 8 cut(s) 277, 682, 1019, 1075, 1147, 1299, 1330, 1417
FalI AAGNNNNNCTT 2 cut(s) 1207, 1239
FaqI GGGAC 2 cut(s) 307, 444
FatI CATG 8 cut(s) 273, 678, 1015, 1071, 1143, 1295, 1326, 1413
FauNDI CATATG 1 cut(s) 190
FbaI TGATCA 1 cut(s) 1068
Fnu4HI GCNGC 6 cut(s) 209, 534, 537, 540, 543, 1486
FokI GGATG 1 cut(s) 1103
FriOI GRGCYC 1 cut(s) 164
Fsp4HI GCNGC 6 cut(s) 209, 534, 537, 540, 543, 1486
FspBI CTAG 4 cut(s) 90, 288, 396, 713
GlaI GCGC 1 cut(s) 54
GluI GCNGC 6 cut(s) 209, 534, 537, 540, 543, 1486
GsuI CTGGAG 2 cut(s) 782, 1365
HaeIII GGCC 4 cut(s) 415, 759, 1502, 1536
HapII CCGG 3 cut(s) 464, 796, 1246
HgaI GACGC 1 cut(s) 1247
HhaI GCGC 1 cut(s) 55
Hin1II CATG 8 cut(s) 277, 682, 1019, 1075, 1147, 1299, 1330, 1417
Hin6I GCGC 1 cut(s) 53
HinP1I GCGC 1 cut(s) 53
HindIII AAGCTT 2 cut(s) 609, 933
HinfI GANTC 7 cut(s) 61, 752, 1027, 1132, 1453, 1549, 1560
HpaII CCGG 3 cut(s) 464, 796, 1246
HphI GGTGA 3 cut(s) 779, 872, 1580
Hpy188I TCNGA 7 cut(s) 14, 850, 970, 997, 1263, 1279, 1398
Hpy188III TCNNGA 6 cut(s) 713, 796, 896, 1107, 1126, 1319
HpyAV CCTTC 8 cut(s) 177, 631, 678, 822, 893, 904, 1070, 1559
HpyCH4III ACNGT 4 cut(s) 523, 743, 1307, 1468
HpyCH4IV ACGT 2 cut(s) 214, 835
HpyF10VI GCNNNNNNNGC 7 cut(s) 421, 436, 539, 542, 1242, 1255, 1349
HpyF3I CTNAG 7 cut(s) 120, 451, 642, 969, 1047, 1395, 1455
HpySE526I ACGT 2 cut(s) 214, 835
Hsp92II CATG 8 cut(s) 277, 682, 1019, 1075, 1147, 1299, 1330, 1417
HspAI GCGC 1 cut(s) 53
Kpn2I TCCGGA 1 cut(s) 795
KpnI GGTACC 1 cut(s) 1175
KroI GCCGGC 1 cut(s) 1245
KroNI GCCGGC 1 cut(s) 1247
Ksp22I TGATCA 1 cut(s) 1068
Kzo9I GATC 9 cut(s) 85, 270, 329, 398, 622, 960, 1068, 1122, 1157
LmnI GCTCC 1 cut(s) 159
Lsp1109I GCAGC 5 cut(s) 195, 520, 529, 548, 1497
LweI GCATC 4 cut(s) 122, 194, 1002, 1389
MaeI CTAG 4 cut(s) 90, 288, 396, 713
MaeII ACGT 2 cut(s) 214, 835
MaeIII GTNAC 5 cut(s) 106, 388, 466, 523, 1375
MalI GATC 9 cut(s) 87, 272, 331, 400, 624, 962, 1070, 1124, 1159
MboI GATC 9 cut(s) 85, 270, 329, 398, 622, 960, 1068, 1122, 1157
MboII GAAGA 6 cut(s) 576, 624, 916, 919, 986, 1181
MfeI CAATTG 1 cut(s) 595
MhlI GDGCHC 1 cut(s) 164
MlsI TGGCCA 1 cut(s) 415
MluCI AATT 5 cut(s) 595, 871, 1116, 1237, 1493
MluNI TGGCCA 1 cut(s) 415
MlyI GAGTC 4 cut(s) 70, 761, 1036, 1447
MmeI TCCRAC 1 cut(s) 491
Mox20I TGGCCA 1 cut(s) 415
Mph1103I ATGCAT 2 cut(s) 694, 1017
MroI TCCGGA 1 cut(s) 795
MroNI GCCGGC 1 cut(s) 1245
MroXI GAANNNNTTC 2 cut(s) 1188, 1306
MscI TGGCCA 1 cut(s) 415
MseI TTAA 4 cut(s) 144, 221, 1490, 1509
Msp20I TGGCCA 1 cut(s) 415
MspA1I CMGCKG 3 cut(s) 455, 542, 725
MspI CCGG 3 cut(s) 464, 796, 1246
MspR9I CCNGG 5 cut(s) 156, 417, 464, 761, 1538
MunI CAATTG 1 cut(s) 595
MvaI CCWGG 4 cut(s) 156, 417, 761, 1538
MvnI CGCG 1 cut(s) 1258
MwoI GCNNNNNNNGC 7 cut(s) 421, 436, 539, 542, 1242, 1255, 1349
NaeI GCCGGC 1 cut(s) 1247
NciI CCSGG 1 cut(s) 464
NdeI CATATG 1 cut(s) 190
NdeII GATC 9 cut(s) 85, 270, 329, 398, 622, 960, 1068, 1122, 1157
NgoMIV GCCGGC 1 cut(s) 1245
NlaIII CATG 8 cut(s) 277, 682, 1019, 1075, 1147, 1299, 1330, 1417
NlaIV GGNNCC 4 cut(s) 97, 460, 676, 1173
NmuCI GTSAC 1 cut(s) 106
NsiI ATGCAT 2 cut(s) 694, 1017
NspV TTCGAA 1 cut(s) 635
PceI AGGCCT 1 cut(s) 759
PdiI GCCGGC 1 cut(s) 1247
PdmI GAANNNNTTC 2 cut(s) 1188, 1306
PfeI GAWTC 3 cut(s) 1132, 1549, 1560
PflMI CCANNNNNTGG 1 cut(s) 655
PkrI GCNGC 6 cut(s) 210, 535, 538, 541, 544, 1487
PleI GAGTC 4 cut(s) 69, 760, 1035, 1447
PpsI GAGTC 4 cut(s) 69, 760, 1035, 1447
Psp124BI GAGCTC 1 cut(s) 164
Psp6I CCWGG 4 cut(s) 154, 415, 759, 1536
PspEI GGTNACC 1 cut(s) 466
PspGI CCWGG 4 cut(s) 154, 415, 759, 1536
PspN4I GGNNCC 4 cut(s) 97, 460, 676, 1173
PspPI GGNCC 2 cut(s) 458, 1501
PstI CTGCAG 2 cut(s) 538, 1011
PstNI CAGNNNCTG 2 cut(s) 267, 542
PvuII CAGCTG 2 cut(s) 455, 725
RsaI GTAC 4 cut(s) 195, 376, 647, 1173
RsaNI GTAC 4 cut(s) 194, 375, 646, 1172
SacI GAGCTC 1 cut(s) 164
SaqAI TTAA 4 cut(s) 144, 221, 1490, 1509
SatI GCNGC 6 cut(s) 209, 534, 537, 540, 543, 1486
Sau3AI GATC 9 cut(s) 85, 270, 329, 398, 622, 960, 1068, 1122, 1157
Sau96I GGNCC 2 cut(s) 458, 1501
SchI GAGTC 4 cut(s) 70, 761, 1036, 1447
ScrFI CCNGG 5 cut(s) 156, 417, 464, 761, 1538
SduI GDGCHC 1 cut(s) 164
SfaNI GCATC 4 cut(s) 122, 194, 1002, 1389
SfcI CTRYAG 3 cut(s) 534, 1007, 1227
SfuI TTCGAA 1 cut(s) 635
SinI GGWCC 1 cut(s) 458
SmlI CTYRAG 1 cut(s) 1460
SmoI CTYRAG 1 cut(s) 1460
Sse9I AATT 5 cut(s) 595, 871, 1116, 1237, 1493
SseBI AGGCCT 1 cut(s) 759
SsiI CCGC 1 cut(s) 540
SspMI CTAG 4 cut(s) 90, 288, 396, 713
SstI GAGCTC 1 cut(s) 164
StuI AGGCCT 1 cut(s) 759
StyD4I CCNGG 5 cut(s) 154, 415, 462, 759, 1536
StyI CCWWGG 1 cut(s) 320
TaaI ACNGT 4 cut(s) 523, 743, 1307, 1468
TaiI ACGT 2 cut(s) 217, 838
TaqI TCGA 4 cut(s) 635, 876, 1125, 1563
TasI AATT 5 cut(s) 595, 871, 1116, 1237, 1493
TatI WGTACW 2 cut(s) 193, 374
TauI GCSGC 1 cut(s) 542
TfiI GAWTC 3 cut(s) 1132, 1549, 1560
Tru1I TTAA 4 cut(s) 144, 221, 1490, 1509
Tru9I TTAA 4 cut(s) 144, 221, 1490, 1509
TscAI CASTG 2 cut(s) 526, 748
TseFI GTSAC 1 cut(s) 106
TseI GCWGC 5 cut(s) 208, 533, 536, 542, 1485
Tsp45I GTSAC 1 cut(s) 106
TspDTI ATGAA 4 cut(s) 576, 1032, 1110, 1284
TspGWI ACGGA 1 cut(s) 1039
TspRI CASTG 2 cut(s) 526, 748
Van91I CCANNNNNTGG 1 cut(s) 655
VpaK11BI GGWCC 1 cut(s) 458
XagI CCTNNNNNAGG 1 cut(s) 324
XapI RAATTY 1 cut(s) 871
XbaI TCTAGA 1 cut(s) 712
XcmI CCANNNNNNNNNTGG 1 cut(s) 1411
XmnI GAANNNNTTC 2 cut(s) 1188, 1306
XspI CTAG 4 cut(s) 90, 288, 396, 713
Zsp2I ATGCAT 2 cut(s) 694, 1017
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.