pycom03g16060

Autophagy-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0006822
Physical Location & Seq
Forward (+)
17242460 .. 17249283
6824 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g16060.6

Sequence Viewer

Length: 2802 bp
ATGATTAAGGCAGTTTTCGAAGAATCATCGGTTTTCGTGATTTGGGTATTGACTAGTTTGATCTCTCTGGTTCTGGGGATGAGGAATGATGGCGGTCAGAAGCAGCAGCAGGGTGGTGTGCCTCGCCCAGCTCGGTCCAACAGCTTCATTCCCAACTCTTTTCGGGCCATTTCGAGCTACTTGAGGATCGTTTCGTCCGGTGCTTCCACCGTTGCGAGGTCCGCGGCTTCGGTGGCTTCCTCGATTGTAGATAGGGATGATGATACCAACCATCGATCAGGCATGGTTAACTGGGCTGGCTTTGACAATTTAGAAGGTGAGGGGAATCTCACTCGTCAAGTTCTCCTTCTGGGTTACCGTTCTGGATTCCAGGTTTGGGATGTTGAAGAAGCAGATAATGTCCGGGACCTGGTTTCCAGATATGATGGTGCTGTCTCATACATGCAAATGCTACCTAAACCTATAGCTTCAAAGAGATCAGAAGACAATTTTGAAGAAAGCCGTCCATTGCTGGTAGTTTGTGCCGATGGATCCATTTCTGTTGGTAATACCATACAGGATGGTAGTGCCACTCCTCGCAATGGTATTACTGCAAACAGCCATGACACAATGAACAGCAGTTTTGTGCCTACTGTTGTTCGGTTTTATTCTCTGAAATCTCAATCTTATGTTCATGTTCTAAAGTTCAGATCAGTTGTTTATTCTTTAAAATGCAGTTCTCGAGTTGTTGCTATTTCTCTAGCAGCTCAGATACACTGTTTTGATGCCATGACATTTGAGAGAGAATATACCATTCTTACCAACCCGATAGTTATGGGATTTCCTGGTTCTGGAGGTATTGGTTGTGGACCTCTTGCATTAGGCAATAGATGGCTGGCTTATAGTGGAAGTCCTGTTGCTGTATCTAATTCTGGGCGTGTCAGTCCACAGCATCTGGAACCTTCTGCAAGTCTTTCTGGTTTTCCTTCAAATGGAAGCCTGGTTGCTCACTATGCAAAAGAGTCAAGCAAGCAACTAGCTGCTGGGATTGTAACCCTGGGAGACATGGGTTATAAGAAGCTGTCCTGGTACTGCTCTGAGCTTATACCTGATAGTAATACTTCTCTTCAATCACCCAATCCTGTGTGGAAAGTCAATGGTACTGTCAATGGCCAGTCCACAGATACTGATAATGTTGGAATGGTCATTGTCAGAGATATTGTCAATAAGGCTGTTATTGCGCAGTTCAGGGCGCACACGAGTCCTATTTCTGCGTTACGCTTTGATCCTAGTGGCACCCTTTTAGTGACTGCATCAACTCAGGGGCATAACGTCAATGTTTTTAAGATAATGCCTGGGAGCTCTCCTCCAACTGATGGTGGTGCATCATATGTACATCTTTACAGGCTGCAACGTGGATTAACAAATGCATTGTTATTATACAGGACATCAGTTTTAGTGATGACAGCAACTGGATCGATCGATAGTTCCTCTAGGGGGACAAGCCATCTGTTTGCAATAAATCCTTGGGGAGGATCAGTAAATCTCCCAACTGCCGATGCTGGTTTTACTACCAAAAGTACAGGATTAGGGGTTACAACTAGATCGTCAGTTCGTTGGCCAGGTTTGCAAACGCCTAATCAGCAAAGCCTTTGCTCAGCTGGTCCCCCGGTTACCCTCTCTGTTGTTAGTAGAATAAGAAATGGAAATAATAGTTGGAGAGGCACTGTAACTGGTGCTGCAGCCGCTGCAACAGGCAAGATGAGTTCCCTTTCTGGGGCAGTTGCTTCTTCATTCCACAATTGCAAAGGCAATGCTTATTATGTGGATCGCAACTCTTCGAAGGCTAAGTACCACCTCTTGGTTTTTTCCCCTTCTGGTTCCATGATACAATATGCATTGAGAATATCAAATGGTCTAGATTTGACAGCTGTGGCTGGATTGAACACTGCTTATGAGTCAGGCCAGGAGGGTGATACAAGATTAGTAGTTGAAGCTATCCGGAAGTGGAATGTATGTCAAAAGCAAAACCGAAGGGAACGTGATGATACTTCTGACATATATGGTGAGAATGTAAATTTCGACAACAATAAGATATTTCCCGAAGGGAAGAAGAAGGGAAACACCATTTACCCTGAAGCTTGCATTACTTTCCCAAAAGCAAAGATCAGTTCTGAGGAAAAACATCAGTTGTATATTTCAGAAGCCGAACTGCAGATGCATGAAGCACGGAGTCCAGTGTGGGCAAAACCTAAGCTATACTTTCAGTCAATGATCATGGAAGGTGTCAAAATGGATGATGAAACCGCCTCAGGAGGGGAAATTGAGATCGAGAGGATTCCTACTTGCATGATTGAAGCAAGATCAAAGGACTTGGTACCAGTTTTTGAATATCTTCAAGCTCCCATATTTCAACAAACAAGGGTTGCTTCTGTAGATAGCAATTTTAGCAGGCAACTTTCGCGTCAGAGGTCTGGTATATCTGAAAATGGTGGGCGTTCATGCAGAAACAGTTCAAGCTCTCTTGACACCATGACTGAAAGTGGTGCTGGAGTGGCAGAACTTACTAATGGGATTGAAGTAACTGGACGGGGTGGTTCTCAGATGCCCACCGAAAGCATGGGCTTTGTAAATAACAATAACAGCCCAAAGACAGAGACTCAGCTTGAGACTGTAAATAATAGAGAGAGCAGCTTAAAATCGGAGGCCCAACTTAAATCTGTAAATAATGACATAGACGGCCTGGGAGTGGGGAATCTGTTTGGAATTGAAGGCGATGAGTTTGATTTGTGCAGATATTCTTTACTTCTACTGGGATATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000226 GO:0000785 GO:0001952 GO:0001953 GO:0003674 GO:0003682 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005730 GO:0005737 GO:0005856 GO:0005874 GO:0005881 GO:0006355 GO:0006357 GO:0006810 GO:0006950 GO:0006996 GO:0007010 GO:0007017 GO:0007030 GO:0007162 GO:0007275 GO:0008047 GO:0008092 GO:0008134 GO:0008150 GO:0009605 GO:0009653 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0009991 GO:0010256 GO:0010468 GO:0010556 GO:0010557 GO:0010594 GO:0010595 GO:0010604 GO:0010628 GO:0010632 GO:0010634 GO:0010638 GO:0010698 GO:0010810 GO:0010812 GO:0015630 GO:0015631 GO:0016043 GO:0016192 GO:0019219 GO:0019222 GO:0019899 GO:0030155 GO:0030234 GO:0030334 GO:0030335 GO:0031023 GO:0031252 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031344 GO:0031346 GO:0031667 GO:0031974 GO:0031981 GO:0032386 GO:0032388 GO:0032501 GO:0032502 GO:0032878 GO:0032879 GO:0032880 GO:0032956 GO:0032970 GO:0033043 GO:0033157 GO:0034260 GO:0035035 GO:0035148 GO:0035239 GO:0035257 GO:0035295 GO:0035327 GO:0040012 GO:0040017 GO:0042221 GO:0042393 GO:0042594 GO:0043085 GO:0043086 GO:0043087 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043547 GO:0043627 GO:0044087 GO:0044089 GO:0044092 GO:0044093 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0045111 GO:0045893 GO:0045935 GO:0045944 GO:0048487 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048646 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051049 GO:0051050 GO:0051128 GO:0051129 GO:0051130 GO:0051171 GO:0051173 GO:0051179 GO:0051222 GO:0051223 GO:0051234 GO:0051239 GO:0051240 GO:0051252 GO:0051254 GO:0051270 GO:0051272 GO:0051336 GO:0051345 GO:0051346 GO:0051427 GO:0051489 GO:0051491 GO:0051493 GO:0051495 GO:0051716 GO:0051893 GO:0051895 GO:0060255 GO:0060341 GO:0060491 GO:0065007 GO:0065009 GO:0070013 GO:0070201 GO:0070887 GO:0071391 GO:0071840 GO:0080090 GO:0090087 GO:0090109 GO:0090316 GO:0090630 GO:0098772 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0120032 GO:0120034 GO:0120035 GO:1901888 GO:1901889 GO:1902680 GO:1903391 GO:1903392 GO:1903506 GO:1903508 GO:1903827 GO:1903829 GO:1904951 GO:2000112 GO:2000114 GO:2000145 GO:2000147 GO:2000249 GO:2000251 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BCAS3 WD40

Protein Analysis

934

Amino Acids

100.75

Weight (kDa)

8.11

Isoelectric Point (pI)

44.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1053
Acc16I TGCGCA 1 cut(s) 1221
Acc65I GGTACC 1 cut(s) 2356
AccB1I GGYRCC 2 cut(s) 1274, 2356
AccB7I CCANNNNNTGG 2 cut(s) 1355, 1840
AccII CGCG 2 cut(s) 224, 2443
AccIII TCCGGA 1 cut(s) 1980
AciI CCGC 5 cut(s) 93, 222, 224, 1725, 2286
AclWI GGATC 7 cut(s) 194, 525, 538, 1259, 1462, 1522, 1815
AcoI YGGCCR 2 cut(s) 1150, 1598
AcsI RAATTY 1 cut(s) 2056
AcuI CTGAAG 1 cut(s) 2136
AfaI GTAC 6 cut(s) 1070, 1141, 1374, 1561, 1832, 2358
AhlI ACTAGT 1 cut(s) 53
AjuI GAANNNNNNNTTGG 2 cut(s) 1678, 1710
Alw21I GWGCWC 1 cut(s) 1343
Alw26I GTCTC 4 cut(s) 439, 1035, 2630, 2642
AlwI GGATC 7 cut(s) 194, 525, 538, 1259, 1462, 1522, 1815
AlwNI CAGNNNCTG 4 cut(s) 934, 1166, 1451, 1727
Ama87I CYCGRG 1 cut(s) 720
Aor13HI TCCGGA 1 cut(s) 1980
AoxI GGCC 6 cut(s) 165, 1150, 1598, 1942, 2685, 2719
ApeKI GCWGC 9 cut(s) 103, 106, 743, 1019, 1387, 1718, 1721, 1727, 2670
ApoI RAATTY 1 cut(s) 2056
Asp700I GAANNNNTTC 2 cut(s) 2373, 2491
Asp718I GGTACC 1 cut(s) 2356
AspLEI GCGC 2 cut(s) 1222, 1234
AspS9I GGNCC 7 cut(s) 135, 165, 219, 406, 848, 1643, 2686
AsuC2I CCSGG 2 cut(s) 404, 1649
AsuHPI GGTGA 4 cut(s) 329, 1104, 1964, 2057
AsuII TTCGAA 2 cut(s) 18, 1820
AvaI CYCGRG 1 cut(s) 720
AvaII GGWCC 5 cut(s) 135, 219, 406, 848, 1643
AxyI CCTNAGG 1 cut(s) 2290
BalI TGGCCA 2 cut(s) 1152, 1600
BamHI GGATCC 1 cut(s) 530
BanI GGYRCC 2 cut(s) 1274, 2356
BanII GRGCYC 1 cut(s) 1343
BarI GAAGNNNNNNTAC 2 cut(s) 1814, 1846
BauI CACGAG 1 cut(s) 1237
BbsI GAAGAC 1 cut(s) 489
Bbv12I GWGCWC 1 cut(s) 1343
BbvI GCAGC 9 cut(s) 115, 118, 755, 1006, 1374, 1705, 1714, 1733, 2682
BccI CCATC 8 cut(s) 83, 279, 419, 521, 554, 864, 1349, 1494
BceAI ACGGC 2 cut(s) 486, 2734
BcgI CGANNNNNNTGC 2 cut(s) 1437, 1471
BclI TGATCA 1 cut(s) 2253
BcnI CCSGG 2 cut(s) 404, 1649
BcoDI GTCTC 4 cut(s) 439, 1035, 2630, 2642
BcuI ACTAGT 1 cut(s) 53
BfaI CTAG 7 cut(s) 54, 740, 1016, 1269, 1473, 1581, 1898
BfmI CTRYAG 4 cut(s) 462, 1719, 2192, 2412
BlpI GCTNAGC 1 cut(s) 1636
Bme18I GGWCC 5 cut(s) 135, 219, 406, 848, 1643
BmeT110I CYCGRG 1 cut(s) 720
BmgT120I GGNCC 7 cut(s) 135, 165, 219, 406, 848, 1643, 2686
BmiI GGNNCC 7 cut(s) 407, 532, 939, 1276, 1645, 1861, 2358
BmrI ACTGGG 2 cut(s) 301, 2801
BmsI GCATC 7 cut(s) 754, 940, 1301, 1373, 1528, 2187, 2574
BmuI ACTGGG 2 cut(s) 301, 2801
BpiI GAAGAC 1 cut(s) 489
BplI GAGNNNNNCTC 2 cut(s) 1330, 1362
BpmI CTGGAG 2 cut(s) 852, 2550
Bpu10I CCTNAGC 1 cut(s) 2232
Bpu1102I GCTNAGC 1 cut(s) 1636
Bpu14I TTCGAA 2 cut(s) 18, 1820
BpuEI CTTGAG 2 cut(s) 202, 2666
BpuMI CCSGG 2 cut(s) 404, 1649
Bsa29I ATCGAT 3 cut(s) 274, 1457, 1461
BsaJI CCNNGG 7 cut(s) 222, 1035, 1036, 1334, 1505, 1647, 2722
BsaWI WCCGGW 2 cut(s) 197, 1980
BsaXI ACNNNNNCTCC 2 cut(s) 1503, 1533
Bse1I ACTGG 8 cut(s) 296, 1153, 1456, 1717, 2216, 2360, 2569, 2796
Bse21I CCTNAGG 1 cut(s) 2290
Bse3DI GCAATG 3 cut(s) 506, 586, 1798
BseAI TCCGGA 1 cut(s) 1980
BseCI ATCGAT 3 cut(s) 274, 1457, 1461
BseDI CCNNGG 7 cut(s) 222, 1035, 1036, 1334, 1505, 1647, 2722
BseGI GGATG 5 cut(s) 84, 262, 385, 565, 2281
BseMI GCAATG 3 cut(s) 506, 586, 1798
BseMII CTCAG 8 cut(s) 761, 1068, 1313, 1650, 2145, 2304, 2594, 2654
BseNI ACTGG 8 cut(s) 296, 1153, 1456, 1717, 2216, 2360, 2569, 2796
BseRI GAGGAG 2 cut(s) 564, 1335
BseXI GCAGC 9 cut(s) 115, 118, 755, 1006, 1374, 1705, 1714, 1733, 2682
BseYI CCCAGC 2 cut(s) 127, 1022
BsgI GTGCAG 1 cut(s) 2791
Bsh1236I CGCG 2 cut(s) 224, 2443
Bsh1285I CGRYCG 1 cut(s) 1461
BshFI GGCC 6 cut(s) 167, 1152, 1600, 1944, 2687, 2721
BshNI GGYRCC 2 cut(s) 1274, 2356
BshVI ATCGAT 3 cut(s) 274, 1457, 1461
BsiEI CGRYCG 1 cut(s) 1461
BsiHKAI GWGCWC 1 cut(s) 1343
BsiHKCI CYCGRG 1 cut(s) 720
BsiSI CCGG 4 cut(s) 198, 403, 1649, 1981
BslFI GGGAC 3 cut(s) 419, 1492, 1629
BsmAI GTCTC 4 cut(s) 439, 1035, 2630, 2642
BsmFI GGGAC 3 cut(s) 419, 1492, 1629
BsnI GGCC 6 cut(s) 167, 1152, 1600, 1944, 2687, 2721
BsoBI CYCGRG 1 cut(s) 720
Bsp119I TTCGAA 2 cut(s) 18, 1820
Bsp1286I GDGCHC 1 cut(s) 1343
Bsp13I TCCGGA 1 cut(s) 1980
Bsp1407I TGTACA 1 cut(s) 1372
Bsp1720I GCTNAGC 1 cut(s) 1636
BspACI CCGC 5 cut(s) 93, 222, 224, 1725, 2286
BspANI GGCC 6 cut(s) 167, 1152, 1600, 1944, 2687, 2721
BspCNI CTCAG 8 cut(s) 760, 1069, 1312, 1649, 2146, 2303, 2593, 2653
BspDI ATCGAT 3 cut(s) 274, 1457, 1461
BspEI TCCGGA 1 cut(s) 1980
BspFNI CGCG 2 cut(s) 224, 2443
BspLI GGNNCC 7 cut(s) 407, 532, 939, 1276, 1645, 1861, 2358
BspMAI CTGCAG 2 cut(s) 1723, 2196
BspPI GGATC 7 cut(s) 194, 525, 538, 1259, 1462, 1522, 1815
BspT104I TTCGAA 2 cut(s) 18, 1820
BspT107I GGYRCC 2 cut(s) 1274, 2356
BsrDI GCAATG 3 cut(s) 506, 586, 1798
BsrGI TGTACA 1 cut(s) 1372
BsrI ACTGG 8 cut(s) 296, 1153, 1456, 1717, 2216, 2360, 2569, 2796
BssECI CCNNGG 7 cut(s) 222, 1035, 1036, 1334, 1505, 1647, 2722
BssSI CACGAG 1 cut(s) 1237
BssT1I CCWWGG 1 cut(s) 1505
Bst2BI CACGAG 1 cut(s) 1237
Bst4CI ACNGT 8 cut(s) 211, 359, 634, 758, 1144, 1708, 2492, 2653
Bst6I CTCTTC 2 cut(s) 1110, 1822
BstAPI GCANNNNNTGC 1 cut(s) 1727
BstAUI TGTACA 1 cut(s) 1372
BstBI TTCGAA 2 cut(s) 18, 1820
BstC8I GCNNGC 5 cut(s) 298, 876, 1010, 2122, 2432
BstDSI CCRYGG 1 cut(s) 222
BstEII GGTNACC 2 cut(s) 353, 1651
BstENI CCTNNNNNAGG 1 cut(s) 1509
BstF5I GGATG 5 cut(s) 84, 262, 385, 565, 2281
BstFNI CGCG 2 cut(s) 224, 2443
BstHHI GCGC 2 cut(s) 1222, 1234
BstMAI GTCTC 4 cut(s) 439, 1035, 2630, 2642
BstMCI CGRYCG 1 cut(s) 1461
BstNSI RCATGY 1 cut(s) 445
BstPI GGTNACC 2 cut(s) 353, 1651
BstSFI CTRYAG 4 cut(s) 462, 1719, 2192, 2412
BstUI CGCG 2 cut(s) 224, 2443
BstV1I GCAGC 9 cut(s) 115, 118, 755, 1006, 1374, 1705, 1714, 1733, 2682
BstV2I GAAGAC 1 cut(s) 489
BstX2I RGATCY 1 cut(s) 530
BstYI RGATCY 1 cut(s) 530
Bsu15I ATCGAT 3 cut(s) 274, 1457, 1461
Bsu36I CCTNAGG 1 cut(s) 2290
BsuRI GGCC 6 cut(s) 167, 1152, 1600, 1944, 2687, 2721
BsuTUI ATCGAT 3 cut(s) 274, 1457, 1461
BtgI CCRYGG 1 cut(s) 222
BtgZI GCGATG 1 cut(s) 2769
BtsCI GGATG 5 cut(s) 84, 262, 385, 565, 2281
BtsI GCAGTG 1 cut(s) 1926
BtsIMutI CAGTG 4 cut(s) 754, 1704, 1926, 2223
Cac8I GCNNGC 5 cut(s) 298, 876, 1010, 2122, 2432
CaiI CAGNNNCTG 4 cut(s) 934, 1166, 1451, 1727
CfoI GCGC 2 cut(s) 1222, 1234
Cfr13I GGNCC 7 cut(s) 135, 165, 219, 406, 848, 1643, 2686
Cfr42I CCGCGG 1 cut(s) 225
ClaI ATCGAT 3 cut(s) 274, 1457, 1461
CseI GACGC 1 cut(s) 2432
CsiI ACCWGGT 1 cut(s) 408
Csp6I GTAC 6 cut(s) 1069, 1140, 1373, 1560, 1831, 2357
CviQI GTAC 6 cut(s) 1069, 1140, 1373, 1560, 1831, 2357
DraI TTTAAA 1 cut(s) 708
EaeI YGGCCR 2 cut(s) 1150, 1598
Eam1104I CTCTTC 2 cut(s) 1110, 1822
EarI CTCTTC 2 cut(s) 1110, 1822
Ecl136II GAGCTC 1 cut(s) 1341
Eco130I CCWWGG 1 cut(s) 1505
Eco24I GRGCYC 1 cut(s) 1343
Eco47I GGWCC 5 cut(s) 135, 219, 406, 848, 1643
Eco53kI GAGCTC 1 cut(s) 1341
Eco57I CTGAAG 1 cut(s) 2136
Eco81I CCTNAGG 1 cut(s) 2290
Eco88I CYCGRG 1 cut(s) 720
Eco91I GGTNACC 2 cut(s) 353, 1651
EcoICRI GAGCTC 1 cut(s) 1341
EcoNI CCTNNNNNAGG 1 cut(s) 1509
EcoO109I RGGNCCY 1 cut(s) 406
EcoO65I GGTNACC 2 cut(s) 353, 1651
EcoT14I CCWWGG 1 cut(s) 1505
EcoT22I ATGCAT 3 cut(s) 1411, 1879, 2202
EcoT38I GRGCYC 1 cut(s) 1343
ErhI CCWWGG 1 cut(s) 1505
FalI AAGNNNNNCTT 6 cut(s) 330, 362, 2225, 2257, 2392, 2424
FaqI GGGAC 3 cut(s) 419, 1492, 1629
FauNDI CATATG 1 cut(s) 1369
FbaI TGATCA 1 cut(s) 2253
FokI GGATG 5 cut(s) 91, 269, 392, 572, 2288
FriOI GRGCYC 1 cut(s) 1343
FspBI CTAG 7 cut(s) 54, 740, 1016, 1269, 1473, 1581, 1898
FspI TGCGCA 1 cut(s) 1221
GlaI GCGC 2 cut(s) 1221, 1233
GsaI CCCAGC 2 cut(s) 131, 1026
GsuI CTGGAG 2 cut(s) 852, 2550
HaeIII GGCC 6 cut(s) 167, 1152, 1600, 1944, 2687, 2721
HapII CCGG 4 cut(s) 198, 403, 1649, 1981
HgaI GACGC 1 cut(s) 2432
HhaI GCGC 2 cut(s) 1222, 1234
Hin6I GCGC 2 cut(s) 1220, 1232
HinP1I GCGC 2 cut(s) 1220, 1232
HincII GTYRAC 1 cut(s) 289
HindII GTYRAC 1 cut(s) 289
HindIII AAGCTT 1 cut(s) 2118
HpaI GTTAAC 1 cut(s) 289
HpaII CCGG 4 cut(s) 198, 403, 1649, 1981
HphI GGTGA 4 cut(s) 329, 1104, 1964, 2057
Hpy166II GTNNAC 4 cut(s) 289, 848, 926, 1158
Hpy8I GTNNAC 4 cut(s) 289, 848, 926, 1158
HpyCH4III ACNGT 8 cut(s) 211, 359, 634, 758, 1144, 1708, 2492, 2653
HpyCH4IV ACGT 3 cut(s) 1311, 1393, 2020
HpySE526I ACGT 3 cut(s) 1311, 1393, 2020
HspAI GCGC 2 cut(s) 1220, 1232
Kpn2I TCCGGA 1 cut(s) 1980
KpnI GGTACC 1 cut(s) 2360
Ksp22I TGATCA 1 cut(s) 2253
KspAI GTTAAC 1 cut(s) 289
KspI CCGCGG 1 cut(s) 225
LmnI GCTCC 2 cut(s) 1338, 2386
Lsp1109I GCAGC 9 cut(s) 115, 118, 755, 1006, 1374, 1705, 1714, 1733, 2682
LweI GCATC 7 cut(s) 754, 940, 1301, 1373, 1528, 2187, 2574
MabI ACCWGGT 1 cut(s) 408
MaeI CTAG 7 cut(s) 54, 740, 1016, 1269, 1473, 1581, 1898
MaeII ACGT 3 cut(s) 1311, 1393, 2020
MaeIII GTNAC 8 cut(s) 353, 1030, 1254, 1285, 1573, 1651, 1708, 2560
MfeI CAATTG 1 cut(s) 1780
MflI RGATCY 1 cut(s) 530
MhlI GDGCHC 1 cut(s) 1343
MlsI TGGCCA 2 cut(s) 1152, 1600
MluCI AATT 8 cut(s) 307, 487, 907, 1780, 2056, 2301, 2422, 2745
MluNI TGGCCA 2 cut(s) 1152, 1600
MlyI GAGTC 5 cut(s) 1010, 1249, 1946, 2221, 2632
MmeI TCCRAC 4 cut(s) 162, 1156, 1373, 1676
Mox20I TGGCCA 2 cut(s) 1152, 1600
Mph1103I ATGCAT 3 cut(s) 1411, 1879, 2202
MroI TCCGGA 1 cut(s) 1980
MroXI GAANNNNTTC 2 cut(s) 2373, 2491
MscI TGGCCA 2 cut(s) 1152, 1600
MseI TTAA 8 cut(s) 6, 288, 707, 1323, 1400, 2675, 2694, 2800
MslI CAYNNNNRTG 1 cut(s) 446
Msp20I TGGCCA 2 cut(s) 1152, 1600
MspA1I CMGCKG 4 cut(s) 224, 1640, 1727, 1910
MspI CCGG 4 cut(s) 198, 403, 1649, 1981
MunI CAATTG 1 cut(s) 1780
MvnI CGCG 2 cut(s) 224, 2443
NciI CCSGG 2 cut(s) 404, 1649
NdeI CATATG 1 cut(s) 1369
NlaIV GGNNCC 7 cut(s) 407, 532, 939, 1276, 1645, 1861, 2358
NmuCI GTSAC 1 cut(s) 1285
NsbI TGCGCA 1 cut(s) 1221
NsiI ATGCAT 3 cut(s) 1411, 1879, 2202
NspI RCATGY 1 cut(s) 445
NspV TTCGAA 2 cut(s) 18, 1820
PaeR7I CTCGAG 1 cut(s) 720
PasI CCCWGGG 1 cut(s) 1036
PdmI GAANNNNTTC 2 cut(s) 2373, 2491
PfeI GAWTC 5 cut(s) 23, 325, 366, 2317, 2734
PflMI CCANNNNNTGG 2 cut(s) 1355, 1840
PfoI TCCNGGA 1 cut(s) 402
Ple19I CGATCG 1 cut(s) 1461
PleI GAGTC 5 cut(s) 1009, 1248, 1945, 2220, 2632
PpsI GAGTC 5 cut(s) 1009, 1248, 1945, 2220, 2632
PpuMI RGGWCCY 1 cut(s) 406
PsiI TTATAA 1 cut(s) 1053
Psp124BI GAGCTC 1 cut(s) 1343
Psp5II RGGWCCY 1 cut(s) 406
PspEI GGTNACC 2 cut(s) 353, 1651
PspFI CCCAGC 2 cut(s) 127, 1022
PspN4I GGNNCC 7 cut(s) 407, 532, 939, 1276, 1645, 1861, 2358
PspPI GGNCC 7 cut(s) 135, 165, 219, 406, 848, 1643, 2686
PspPPI RGGWCCY 1 cut(s) 406
PstI CTGCAG 2 cut(s) 1723, 2196
PstNI CAGNNNCTG 4 cut(s) 934, 1166, 1451, 1727
PsuI RGATCY 1 cut(s) 530
PvuI CGATCG 1 cut(s) 1461
PvuII CAGCTG 2 cut(s) 1640, 1910
RsaI GTAC 6 cut(s) 1070, 1141, 1374, 1561, 1832, 2358
RsaNI GTAC 6 cut(s) 1069, 1140, 1373, 1560, 1831, 2357
RseI CAYNNNNRTG 1 cut(s) 446
SacI GAGCTC 1 cut(s) 1343
SacII CCGCGG 1 cut(s) 225
SaqAI TTAA 8 cut(s) 6, 288, 707, 1323, 1400, 2675, 2694, 2800
Sau96I GGNCC 7 cut(s) 135, 165, 219, 406, 848, 1643, 2686
SchI GAGTC 5 cut(s) 1010, 1249, 1946, 2221, 2632
SduI GDGCHC 1 cut(s) 1343
SexAI ACCWGGT 1 cut(s) 408
SfaNI GCATC 7 cut(s) 754, 940, 1301, 1373, 1528, 2187, 2574
SfcI CTRYAG 4 cut(s) 462, 1719, 2192, 2412
Sfr274I CTCGAG 1 cut(s) 720
Sfr303I CCGCGG 1 cut(s) 225
SfuI TTCGAA 2 cut(s) 18, 1820
SgrBI CCGCGG 1 cut(s) 225
SinI GGWCC 5 cut(s) 135, 219, 406, 848, 1643
SlaI CTCGAG 1 cut(s) 720
SmiMI CAYNNNNRTG 1 cut(s) 446
SmlI CTYRAG 3 cut(s) 181, 720, 2645
SmoI CTYRAG 3 cut(s) 181, 720, 2645
SpeI ACTAGT 1 cut(s) 53
Sse9I AATT 8 cut(s) 307, 487, 907, 1780, 2056, 2301, 2422, 2745
SsiI CCGC 5 cut(s) 93, 222, 224, 1725, 2286
SspMI CTAG 7 cut(s) 54, 740, 1016, 1269, 1473, 1581, 1898
SstI GAGCTC 1 cut(s) 1343
StyI CCWWGG 1 cut(s) 1505
TaaI ACNGT 8 cut(s) 211, 359, 634, 758, 1144, 1708, 2492, 2653
TaiI ACGT 3 cut(s) 1314, 1396, 2023
TaqII GACCGA 1 cut(s) 123
TasI AATT 8 cut(s) 307, 487, 907, 1780, 2056, 2301, 2422, 2745
TatI WGTACW 2 cut(s) 1372, 1559
TauI GCSGC 2 cut(s) 227, 1727
TfiI GAWTC 5 cut(s) 23, 325, 366, 2317, 2734
Tru1I TTAA 8 cut(s) 6, 288, 707, 1323, 1400, 2675, 2694, 2800
Tru9I TTAA 8 cut(s) 6, 288, 707, 1323, 1400, 2675, 2694, 2800
TscAI CASTG 4 cut(s) 761, 1711, 1933, 2223
TseFI GTSAC 1 cut(s) 1285
TseI GCWGC 9 cut(s) 103, 106, 743, 1019, 1387, 1718, 1721, 1727, 2670
Tsp45I GTSAC 1 cut(s) 1285
TspDTI ATGAA 7 cut(s) 136, 626, 662, 1761, 2217, 2295, 2469
TspGWI ACGGA 1 cut(s) 2224
TspRI CASTG 4 cut(s) 761, 1711, 1933, 2223
Van91I CCANNNNNTGG 2 cut(s) 1355, 1840
VpaK11BI GGWCC 5 cut(s) 135, 219, 406, 848, 1643
XagI CCTNNNNNAGG 1 cut(s) 1509
XapI RAATTY 1 cut(s) 2056
XbaI TCTAGA 1 cut(s) 1897
XceI RCATGY 1 cut(s) 445
XcmI CCANNNNNNNNNTGG 1 cut(s) 2596
XhoI CTCGAG 1 cut(s) 720
XmnI GAANNNNTTC 2 cut(s) 2373, 2491
XspI CTAG 7 cut(s) 54, 740, 1016, 1269, 1473, 1581, 1898
Zsp2I ATGCAT 3 cut(s) 1411, 1879, 2202
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.