Rh5DG264000

Autophagy-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0006822
Physical Location & Seq
Reverse (-)
31688197 .. 31696760
8564 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG264000.1

Sequence Viewer

Length: 2316 bp
ATGATGAATGATGCTGGCCGGAATGGTGGTGGTGTTTCCCGCCATGCCAAGACCAACGGCTTCATTCCAAGCTCGTTTCGGGCGCTTTCGAGTTATCTGAGAATTGTTTCTTCTGGGGCTTCAACTGTTGCCAGGTCGGCGGTGTCGGCGGCCTCTTCTATTGCGGAGAGGGATAATGATGCCAACCATGATCAGGTAAACTGGGCTGGATTTGACAAGTTAGAGGGGGAAAGAAATGTCTACCGGAAAGTTCTTCTACTGGGTTACCGCTCTGGTTTCCAGGTTTGGGATGTTGAAGAAGCAGATAATGTCCGTGACCTGGTTTCCACACATGATGGTCCTATTTCATTTATGCAAATCCTACCTAAACCAATAGCATCAAAGAGATCGGATGACAAGTTTGCTGAAAGCCGTCCATTGCTGGTAGTTTGTGCTGATGGGTCAATTTCTGTTGATAATAACATAGAGGATGGCACTGCCCCTCGCAATGGTGTTACTACAAACAGCCATGACAAAGTGAACGGTAGTTTTGTGCCTACTGCTGTTCGGTTCTATTCCTTGAGATCCCAATCCTATGTTCATGTGCTGAAGTTCAGATCAGCTATTTATTCTGTAAAGTGCAGTTCTCGAGTTGTTGCTATTTCTCAAGCAACTCAGATACACTGTTTTGATGCAACAACGTTGGAGCGGGAATATACTATTCTCACCAATCCTATAGTTACGGGATTCCCTGGTTCTGGAGGTATAAGCTGTGGACCTGTTGCAGTAGGCAAGAGATGGCTAGCTTATAGTGGAAGTCCTGTTGCAGTAACCAATTCTGGGCGCGTTAATCCACAACATCTGGCACCTTCCGCTAGTTTTCCTTCAAATGGGAGCCTGGTTGCTCACTATGCAAAAGAGTCAAGCAAGCAACTAGCTACTGGGATTGCGACCTTGGGAGACATGGGGTATAAGAAGCTGTCCCAGTACTACTCGGAGCTTGTACCTGATGGTATTACTTATCCTCAAGCAGGGAATCCTGGCCGGAAACCCAATGGAACTGTCAATGGCCTGTCAACAGACACAGATAGTGTTGGAATGGTCATTGTCAGAGACATTGTCAGTAAAGCTGTTATTGCACAGTTCAGGGCTCACCAGAGTCCTATTTCTGCATTATGCTTTGATCCTAGTGGCACACTTTTAGTGTCTGCTTCGGTTCAGGGGCATAACATCAATGTTTTTAAGATAATGCCTGGACCTCCTGGAGTCTCCTCTTCAACTGATGCTGATGCATCTTATGTACATCTTTACAGGCTACAGCGCGGAATGACACATGCAATTATTCAGGACATTAGTTTCAGTGACGACAGCAACTGGATTATGGTTAGTTCCTCTAGGGGGACCAGCCATCTGTTTGCTATAAATCCTTGGGGAGGAACTGTTGATATTCCAAATTCTGATGCTAGCTTTAATCCAAAAAATACTGTATCAGGTGTCACAACTAGGTCAGCAGTTCGTTGGCCACCTAATTCAGGTTCGCAAATGCCTAATCAGCAGAGCCTCTGTTCATCTGGTCCCCCAGTTACACTTTCTGTTGTTAGTAGAATAAGAAATGGAAACAATAGCTGGAGAGGCACTGTAACTGGTGCTGCGGCAGCCGCCACTGGCAAGATGAATTCCCTCTCTGGGGCAATTGCTTCCTCTTTCCATAATTGCAAAGGCAATGATTACCATGTGGATTCCAGCTCTTTGAAGGCTAAGTACCACCTTTTGGTATTTTCTCCATCTGGTTGCATGATACAATATGCATTGCGAATACCAAATGGTCTAGATTTAACAACTGTGACTGGATTAAACACTGCTTATGAGTCAGATCACGAGTGTGATGCAAGATTAGTAGTTGAAGCTATCCAGAAGTGGAACGTATGTCAGAAGCAGAACCGAAGAGAACGGGAGGATAATACTGACATATATGGTGAGAATGGAAATTTAGACAACAATAAGATATATCCTGAAGGGATGAAGAAGGGAAACACAGTATATCCTGAAGCTTGGAGTTCTGCCACAAAAGAAAAGATCACCCCTGAGGAAAAACATCATTTGTATATTTCAGAAGCCGAACTGCAGATGCATGAAACTCGAAGTCCACTATGGGCAAGACCTGAGCTATACTTTCAGTCAATGATTGTGGAAGGTGTGAAGATGGATGGTGAAACTGCTACAGGAGGAGAAATTGAGATCGAAAGGATTCCGACTCGCATGATTGAAGCAAGGTCAAAGGATTTAGTCCCAGTTTTTGACTATCTTCAGACTCCAGTGTTCCAACAAACAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000226 GO:0000785 GO:0001952 GO:0001953 GO:0003674 GO:0003682 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005730 GO:0005737 GO:0005856 GO:0005874 GO:0005881 GO:0006355 GO:0006357 GO:0006810 GO:0006950 GO:0006996 GO:0007010 GO:0007017 GO:0007030 GO:0007162 GO:0007275 GO:0008047 GO:0008092 GO:0008134 GO:0008150 GO:0009605 GO:0009653 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0009991 GO:0010256 GO:0010468 GO:0010556 GO:0010557 GO:0010594 GO:0010595 GO:0010604 GO:0010628 GO:0010632 GO:0010634 GO:0010638 GO:0010698 GO:0010810 GO:0010812 GO:0015630 GO:0015631 GO:0016043 GO:0016192 GO:0019219 GO:0019222 GO:0019899 GO:0030155 GO:0030234 GO:0030334 GO:0030335 GO:0031023 GO:0031252 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031344 GO:0031346 GO:0031667 GO:0031974 GO:0031981 GO:0032386 GO:0032388 GO:0032501 GO:0032502 GO:0032878 GO:0032879 GO:0032880 GO:0032956 GO:0032970 GO:0033043 GO:0033157 GO:0034260 GO:0035035 GO:0035148 GO:0035239 GO:0035257 GO:0035295 GO:0035327 GO:0040012 GO:0040017 GO:0042221 GO:0042393 GO:0042594 GO:0043085 GO:0043086 GO:0043087 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043547 GO:0043627 GO:0044087 GO:0044089 GO:0044092 GO:0044093 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0045111 GO:0045893 GO:0045935 GO:0045944 GO:0048487 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048646 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051049 GO:0051050 GO:0051128 GO:0051129 GO:0051130 GO:0051171 GO:0051173 GO:0051179 GO:0051222 GO:0051223 GO:0051234 GO:0051239 GO:0051240 GO:0051252 GO:0051254 GO:0051270 GO:0051272 GO:0051336 GO:0051345 GO:0051346 GO:0051427 GO:0051489 GO:0051491 GO:0051493 GO:0051495 GO:0051716 GO:0051893 GO:0051895 GO:0060255 GO:0060341 GO:0060491 GO:0065007 GO:0065009 GO:0070013 GO:0070201 GO:0070887 GO:0071391 GO:0071840 GO:0080090 GO:0090087 GO:0090109 GO:0090316 GO:0090630 GO:0098772 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0120032 GO:0120034 GO:0120035 GO:1901888 GO:1901889 GO:1902680 GO:1903391 GO:1903392 GO:1903506 GO:1903508 GO:1903827 GO:1903829 GO:1904951 GO:2000112 GO:2000114 GO:2000145 GO:2000147 GO:2000249 GO:2000251 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BCAS3 WD40

Protein Analysis

771

Amino Acids

83.63

Weight (kDa)

7.95

Isoelectric Point (pI)

42.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BCAS3_WD40 PF21034 70 - 473 1.8e-49 BCAS3, WD40 repeat
PROPPIN PF21032 359 - 468 6e-06 PROPPIN
BCAS3 PF12490 615 - 760 2.5e-55 BCAS3 microtubule associated cell migration factor, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 844
AccB7I CCANNNNNTGG 1 cut(s) 1750
AccBSI CCGCTC 2 cut(s) 270, 688
AccI GTMKAC 1 cut(s) 240
AccII CGCG 2 cut(s) 825, 1302
AclI AACGTT 1 cut(s) 680
AclWI GGATC 2 cut(s) 558, 1157
AcoI YGGCCR 3 cut(s) 16, 1021, 1499
AcsI RAATTY 3 cut(s) 1432, 1654, 1966
AcuI CTGAAG 4 cut(s) 608, 2013, 2046, 2270
AfaI GTAC 4 cut(s) 968, 984, 1281, 1742
AgsI TTSAA 7 cut(s) 123, 296, 867, 1257, 1732, 1883, 2246
AjnI CCWGG 8 cut(s) 131, 279, 318, 730, 876, 1018, 1231, 1240
AjuI GAANNNNNNNTTGG 2 cut(s) 561, 593
AleI CACNNNNGTG 1 cut(s) 1860
Alw26I GTCTC 3 cut(s) 933, 1086, 1252
AlwI GGATC 2 cut(s) 558, 1157
AlwNI CAGNNNCTG 1 cut(s) 1353
Ama87I CYCGRG 1 cut(s) 627
AoxI GGCC 5 cut(s) 16, 150, 1021, 1048, 1499
ApeKI GCWGC 2 cut(s) 1628, 1634
ApoI RAATTY 3 cut(s) 1432, 1654, 1966
Asp700I GAANNNNTTC 2 cut(s) 106, 2226
AspLEI GCGC 3 cut(s) 85, 825, 1302
AspS9I GGNCC 5 cut(s) 338, 755, 1235, 1380, 1553
AsuHPI GGTGA 5 cut(s) 697, 1124, 1967, 2050, 2201
AsuNHI GCTAGC 2 cut(s) 781, 1442
AvaI CYCGRG 1 cut(s) 627
AvaII GGWCC 5 cut(s) 338, 755, 1235, 1380, 1553
AxyI CCTNAGG 1 cut(s) 2064
BalI TGGCCA 1 cut(s) 1501
BanI GGYRCC 1 cut(s) 844
BanII GRGCYC 1 cut(s) 1132
BarI GAAGNNNNNNTAC 2 cut(s) 1724, 1756
BauI CACGAG 1 cut(s) 1856
BbvI GCAGC 2 cut(s) 1615, 1646
BccI CCATC 9 cut(s) 329, 431, 464, 771, 983, 1395, 1771, 2176, 2180
BceAI ACGGC 2 cut(s) 73, 396
BcgI CGANNNNNNTGC 4 cut(s) 1847, 1881, 2099, 2133
BciT130I CCWGG 8 cut(s) 133, 281, 320, 732, 878, 1020, 1233, 1242
BclI TGATCA 1 cut(s) 190
BcoDI GTCTC 3 cut(s) 933, 1086, 1252
BfaI CTAG 8 cut(s) 782, 855, 914, 1167, 1374, 1443, 1482, 1808
BfmI CTRYAG 4 cut(s) 714, 1295, 2102, 2199
BfoI RGCGCY 1 cut(s) 86
BglI GCCNNNNNGGC 1 cut(s) 137
BisI GCNGC 5 cut(s) 150, 1629, 1632, 1635, 1638
BlsI GCNGC 5 cut(s) 151, 1630, 1633, 1636, 1639
BmcAI AGTACT 1 cut(s) 968
Bme1390I CCNGG 8 cut(s) 133, 281, 320, 732, 878, 1020, 1233, 1242
Bme18I GGWCC 5 cut(s) 338, 755, 1235, 1380, 1553
BmeT110I CYCGRG 1 cut(s) 627
BmgT120I GGNCC 5 cut(s) 338, 755, 1235, 1380, 1553
BmiI GGNNCC 4 cut(s) 846, 875, 1381, 1555
BmrFI CCNGG 8 cut(s) 133, 281, 320, 732, 878, 1020, 1233, 1242
BmrI ACTGGG 6 cut(s) 211, 269, 930, 958, 1553, 2264
BmsI GCATC 9 cut(s) 169, 386, 661, 1252, 1258, 1280, 1429, 1855, 2097
BmtI GCTAGC 2 cut(s) 785, 1446
BmuI ACTGGG 6 cut(s) 211, 269, 930, 958, 1553, 2264
BpmI CTGGAG 4 cut(s) 759, 1263, 1627, 2277
Bpu10I CCTNAGC 1 cut(s) 2142
BpuEI CTTGAG 3 cut(s) 580, 630, 990
BsaBI GATNNNNATC 1 cut(s) 568
BsaJI CCNNGG 3 cut(s) 730, 933, 1406
BsaWI WCCGGW 1 cut(s) 243
BsaXI ACNNNNNCTCC 2 cut(s) 1404, 1434
Bse21I CCTNAGG 1 cut(s) 2064
Bse3DI GCAATG 4 cut(s) 416, 493, 1708, 1787
Bse8I GATNNNNATC 1 cut(s) 568
BseBI CCWGG 8 cut(s) 133, 281, 320, 732, 878, 1020, 1233, 1242
BseDI CCNNGG 3 cut(s) 730, 933, 1406
BseGI GGATG 5 cut(s) 295, 397, 475, 2004, 2191
BseJI GATNNNNATC 1 cut(s) 568
BseMI GCAATG 4 cut(s) 416, 493, 1708, 1787
BseMII CTCAG 4 cut(s) 89, 668, 2055, 2133
BseRI GAGGAG 2 cut(s) 1240, 2220
BseXI GCAGC 2 cut(s) 1615, 1646
BsgI GTGCAG 1 cut(s) 640
Bsh1236I CGCG 2 cut(s) 825, 1302
BshFI GGCC 5 cut(s) 18, 152, 1023, 1050, 1501
BshNI GGYRCC 1 cut(s) 844
BsiHKCI CYCGRG 1 cut(s) 627
BsiSI CCGG 3 cut(s) 19, 244, 1024
BslFI GGGAC 4 cut(s) 946, 1393, 1539, 2252
BsmAI GTCTC 3 cut(s) 933, 1086, 1252
BsmFI GGGAC 4 cut(s) 946, 1393, 1539, 2252
BsnI GGCC 5 cut(s) 18, 152, 1023, 1050, 1501
BsoBI CYCGRG 1 cut(s) 627
Bsp1286I GDGCHC 1 cut(s) 1132
Bsp1407I TGTACA 1 cut(s) 1279
Bsp143I GATC 8 cut(s) 190, 386, 563, 596, 1162, 1852, 2055, 2217
BspANI GGCC 5 cut(s) 18, 152, 1023, 1050, 1501
BspCNI CTCAG 4 cut(s) 90, 667, 2056, 2134
BspFNI CGCG 2 cut(s) 825, 1302
BspLI GGNNCC 4 cut(s) 846, 875, 1381, 1555
BspMAI CTGCAG 1 cut(s) 2106
BspOI GCTAGC 2 cut(s) 785, 1446
BspPI GGATC 2 cut(s) 558, 1157
BspT107I GGYRCC 1 cut(s) 844
BsrBI CCGCTC 2 cut(s) 270, 688
BsrDI GCAATG 4 cut(s) 416, 493, 1708, 1787
BsrGI TGTACA 1 cut(s) 1279
BssECI CCNNGG 3 cut(s) 730, 933, 1406
BssMI GATC 8 cut(s) 190, 386, 563, 596, 1162, 1852, 2055, 2217
BssSI CACGAG 1 cut(s) 1856
BssT1I CCWWGG 2 cut(s) 933, 1406
Bst2BI CACGAG 1 cut(s) 1856
Bst2UI CCWGG 8 cut(s) 133, 281, 320, 732, 878, 1020, 1233, 1242
Bst6I CTCTTC 3 cut(s) 160, 1258, 1918
BstAUI TGTACA 1 cut(s) 1279
BstC8I GCNNGC 4 cut(s) 16, 783, 908, 1444
BstDEI CTNAG 5 cut(s) 98, 654, 1737, 2064, 2142
BstEII GGTNACC 1 cut(s) 263
BstENI CCTNNNNNAGG 3 cut(s) 1008, 1410, 1509
BstF5I GGATG 5 cut(s) 295, 397, 475, 2004, 2191
BstFNI CGCG 2 cut(s) 825, 1302
BstH2I RGCGCY 1 cut(s) 86
BstHHI GCGC 3 cut(s) 85, 825, 1302
BstKTI GATC 8 cut(s) 193, 389, 566, 599, 1165, 1855, 2058, 2220
BstMAI GTCTC 3 cut(s) 933, 1086, 1252
BstMBI GATC 8 cut(s) 190, 386, 563, 596, 1162, 1852, 2055, 2217
BstMWI GCNNNNNNNGC 9 cut(s) 137, 146, 851, 890, 1115, 1531, 1611, 1634, 1637
BstNI CCWGG 8 cut(s) 133, 281, 320, 732, 878, 1020, 1233, 1242
BstNSI RCATGY 1 cut(s) 1316
BstPI GGTNACC 1 cut(s) 263
BstSCI CCNGG 8 cut(s) 131, 279, 318, 730, 876, 1018, 1231, 1240
BstSFI CTRYAG 4 cut(s) 714, 1295, 2102, 2199
BstUI CGCG 2 cut(s) 825, 1302
BstV1I GCAGC 2 cut(s) 1615, 1646
BstX2I RGATCY 1 cut(s) 563
BstYI RGATCY 1 cut(s) 563
Bsu36I CCTNAGG 1 cut(s) 2064
BsuRI GGCC 5 cut(s) 18, 152, 1023, 1050, 1501
BtsCI GGATG 5 cut(s) 295, 397, 475, 2004, 2191
BtsI GCAGTG 2 cut(s) 474, 1836
BtsIMutI CAGTG 7 cut(s) 474, 661, 1345, 1614, 1641, 1836, 2301
Cac8I GCNNGC 4 cut(s) 16, 783, 908, 1444
CaiI CAGNNNCTG 1 cut(s) 1353
CfoI GCGC 3 cut(s) 85, 825, 1302
Cfr13I GGNCC 5 cut(s) 338, 755, 1235, 1380, 1553
CsiI ACCWGGT 1 cut(s) 318
Csp6I GTAC 4 cut(s) 967, 983, 1280, 1741
CspCI CAANNNNNGTGG 2 cut(s) 2148, 2183
CviQI GTAC 4 cut(s) 967, 983, 1280, 1741
DdeI CTNAG 5 cut(s) 98, 654, 1737, 2064, 2142
DpnI GATC 8 cut(s) 192, 388, 565, 598, 1164, 1854, 2057, 2219
DpnII GATC 8 cut(s) 190, 386, 563, 596, 1162, 1852, 2055, 2217
EaeI YGGCCR 3 cut(s) 16, 1021, 1499
Eam1104I CTCTTC 3 cut(s) 160, 1258, 1918
EarI CTCTTC 3 cut(s) 160, 1258, 1918
Eco130I CCWWGG 2 cut(s) 933, 1406
Eco24I GRGCYC 1 cut(s) 1132
Eco47I GGWCC 5 cut(s) 338, 755, 1235, 1380, 1553
Eco57I CTGAAG 4 cut(s) 608, 2013, 2046, 2270
Eco81I CCTNAGG 1 cut(s) 2064
Eco88I CYCGRG 1 cut(s) 627
Eco91I GGTNACC 1 cut(s) 263
EcoNI CCTNNNNNAGG 3 cut(s) 1008, 1410, 1509
EcoO65I GGTNACC 1 cut(s) 263
EcoRI GAATTC 1 cut(s) 1654
EcoRII CCWGG 8 cut(s) 131, 279, 318, 730, 876, 1018, 1231, 1240
EcoT14I CCWWGG 2 cut(s) 933, 1406
EcoT22I ATGCAT 3 cut(s) 1273, 1789, 2112
EcoT38I GRGCYC 1 cut(s) 1132
ErhI CCWWGG 2 cut(s) 933, 1406
FaqI GGGAC 4 cut(s) 946, 1393, 1539, 2252
FauI CCCGC 2 cut(s) 47, 681
FbaI TGATCA 1 cut(s) 190
FblI GTMKAC 1 cut(s) 240
Fnu4HI GCNGC 5 cut(s) 150, 1629, 1632, 1635, 1638
FokI GGATG 5 cut(s) 302, 404, 482, 2011, 2198
FriOI GRGCYC 1 cut(s) 1132
Fsp4HI GCNGC 5 cut(s) 150, 1629, 1632, 1635, 1638
FspBI CTAG 8 cut(s) 782, 855, 914, 1167, 1374, 1443, 1482, 1808
GlaI GCGC 3 cut(s) 84, 824, 1301
GluI GCNGC 5 cut(s) 150, 1629, 1632, 1635, 1638
GsuI CTGGAG 4 cut(s) 759, 1263, 1627, 2277
HaeII RGCGCY 1 cut(s) 86
HaeIII GGCC 5 cut(s) 18, 152, 1023, 1050, 1501
HapII CCGG 3 cut(s) 19, 244, 1024
HhaI GCGC 3 cut(s) 85, 825, 1302
Hin6I GCGC 3 cut(s) 83, 823, 1300
HinP1I GCGC 3 cut(s) 83, 823, 1300
HincII GTYRAC 1 cut(s) 1056
HindII GTYRAC 1 cut(s) 1056
HindIII AAGCTT 1 cut(s) 2028
HpaII CCGG 3 cut(s) 19, 244, 1024
HphI GGTGA 5 cut(s) 697, 1124, 1967, 2050, 2201
Hpy166II GTNNAC 6 cut(s) 199, 241, 520, 755, 1056, 2126
Hpy188III TCNNGA 8 cut(s) 627, 738, 1325, 1808, 1856, 1891, 1991, 2024
Hpy8I GTNNAC 6 cut(s) 199, 241, 520, 755, 1056, 2126
HpyAV CCTTC 6 cut(s) 858, 873, 1726, 1988, 1999, 2165
HpyCH4IV ACGT 2 cut(s) 680, 1902
HpyF10VI GCNNNNNNNGC 9 cut(s) 137, 146, 851, 890, 1115, 1531, 1611, 1634, 1637
HpyF3I CTNAG 5 cut(s) 98, 654, 1737, 2064, 2142
HpySE526I ACGT 2 cut(s) 680, 1902
HspAI GCGC 3 cut(s) 83, 823, 1300
Ksp22I TGATCA 1 cut(s) 190
Kzo9I GATC 8 cut(s) 190, 386, 563, 596, 1162, 1852, 2055, 2217
LmnI GCTCC 3 cut(s) 685, 873, 976
Lsp1109I GCAGC 2 cut(s) 1615, 1646
LweI GCATC 9 cut(s) 169, 386, 661, 1252, 1258, 1280, 1429, 1855, 2097
MabI ACCWGGT 1 cut(s) 318
MaeI CTAG 8 cut(s) 782, 855, 914, 1167, 1374, 1443, 1482, 1808
MaeII ACGT 2 cut(s) 680, 1902
MalI GATC 8 cut(s) 192, 388, 565, 598, 1164, 1854, 2057, 2219
MbiI CCGCTC 2 cut(s) 270, 688
MboI GATC 8 cut(s) 190, 386, 563, 596, 1162, 1852, 2055, 2217
MboII GAAGA 9 cut(s) 102, 147, 245, 308, 1245, 1935, 2014, 2191, 2276
MfeI CAATTG 1 cut(s) 1671
MflI RGATCY 1 cut(s) 563
MhlI GDGCHC 1 cut(s) 1132
MlsI TGGCCA 1 cut(s) 1501
MluNI TGGCCA 1 cut(s) 1501
MlyI GAGTC 6 cut(s) 908, 1147, 1254, 1856, 2227, 2284
MmeI TCCRAC 3 cut(s) 663, 1054, 2255
Mox20I TGGCCA 1 cut(s) 1501
Mph1103I ATGCAT 3 cut(s) 1273, 1789, 2112
MroXI GAANNNNTTC 2 cut(s) 106, 2226
MscI TGGCCA 1 cut(s) 1501
MseI TTAA 5 cut(s) 828, 1221, 1449, 1814, 1832
MslI CAYNNNNRTG 1 cut(s) 1860
Msp20I TGGCCA 1 cut(s) 1501
MspI CCGG 3 cut(s) 19, 244, 1024
MspR9I CCNGG 8 cut(s) 133, 281, 320, 732, 878, 1020, 1233, 1242
MunI CAATTG 1 cut(s) 1671
MvaI CCWGG 8 cut(s) 133, 281, 320, 732, 878, 1020, 1233, 1242
MvnI CGCG 2 cut(s) 825, 1302
MwoI GCNNNNNNNGC 9 cut(s) 137, 146, 851, 890, 1115, 1531, 1611, 1634, 1637
NdeII GATC 8 cut(s) 190, 386, 563, 596, 1162, 1852, 2055, 2217
NheI GCTAGC 2 cut(s) 781, 1442
NlaIV GGNNCC 4 cut(s) 846, 875, 1381, 1555
NmuCI GTSAC 4 cut(s) 314, 1340, 1474, 1822
NsiI ATGCAT 3 cut(s) 1273, 1789, 2112
NspI RCATGY 1 cut(s) 1316
OliI CACNNNNGTG 1 cut(s) 1860
PaeR7I CTCGAG 1 cut(s) 627
PdmI GAANNNNTTC 2 cut(s) 106, 2226
PfeI GAWTC 4 cut(s) 726, 1015, 1718, 2227
PflFI GACNNNGTC 1 cut(s) 1097
PflMI CCANNNNNTGG 1 cut(s) 1750
PfoI TCCNGGA 1 cut(s) 1240
PkrI GCNGC 5 cut(s) 151, 1630, 1633, 1636, 1639
PleI GAGTC 6 cut(s) 907, 1146, 1253, 1855, 2227, 2284
PpsI GAGTC 6 cut(s) 907, 1146, 1253, 1855, 2227, 2284
Psp1406I AACGTT 1 cut(s) 680
Psp6I CCWGG 8 cut(s) 131, 279, 318, 730, 876, 1018, 1231, 1240
PspEI GGTNACC 1 cut(s) 263
PspGI CCWGG 8 cut(s) 131, 279, 318, 730, 876, 1018, 1231, 1240
PspN4I GGNNCC 4 cut(s) 846, 875, 1381, 1555
PspPI GGNCC 5 cut(s) 338, 755, 1235, 1380, 1553
PstI CTGCAG 1 cut(s) 2106
PstNI CAGNNNCTG 1 cut(s) 1353
PsuI RGATCY 1 cut(s) 563
PsyI GACNNNGTC 1 cut(s) 1097
RsaI GTAC 4 cut(s) 968, 984, 1281, 1742
RsaNI GTAC 4 cut(s) 967, 983, 1280, 1741
RseI CAYNNNNRTG 1 cut(s) 1860
SaqAI TTAA 5 cut(s) 828, 1221, 1449, 1814, 1832
SatI GCNGC 5 cut(s) 150, 1629, 1632, 1635, 1638
Sau3AI GATC 8 cut(s) 190, 386, 563, 596, 1162, 1852, 2055, 2217
Sau96I GGNCC 5 cut(s) 338, 755, 1235, 1380, 1553
ScaI AGTACT 1 cut(s) 968
SchI GAGTC 6 cut(s) 908, 1147, 1254, 1856, 2227, 2284
ScrFI CCNGG 8 cut(s) 133, 281, 320, 732, 878, 1020, 1233, 1242
SduI GDGCHC 1 cut(s) 1132
SexAI ACCWGGT 1 cut(s) 318
SfaNI GCATC 9 cut(s) 169, 386, 661, 1252, 1258, 1280, 1429, 1855, 2097
SfcI CTRYAG 4 cut(s) 714, 1295, 2102, 2199
Sfr274I CTCGAG 1 cut(s) 627
SinI GGWCC 5 cut(s) 338, 755, 1235, 1380, 1553
SlaI CTCGAG 1 cut(s) 627
SmiMI CAYNNNNRTG 1 cut(s) 1860
SmlI CTYRAG 4 cut(s) 559, 627, 645, 1005
SmoI CTYRAG 4 cut(s) 559, 627, 645, 1005
SspMI CTAG 8 cut(s) 782, 855, 914, 1167, 1374, 1443, 1482, 1808
StyD4I CCNGG 8 cut(s) 131, 279, 318, 730, 876, 1018, 1231, 1240
StyI CCWWGG 2 cut(s) 933, 1406
TaiI ACGT 2 cut(s) 683, 1905
TaqI TCGA 4 cut(s) 89, 628, 2119, 2220
TatI WGTACW 2 cut(s) 966, 1279
TauI GCSGC 3 cut(s) 152, 1634, 1640
TfiI GAWTC 4 cut(s) 726, 1015, 1718, 2227
Tru1I TTAA 5 cut(s) 828, 1221, 1449, 1814, 1832
Tru9I TTAA 5 cut(s) 828, 1221, 1449, 1814, 1832
TscAI CASTG 7 cut(s) 481, 668, 1345, 1621, 1648, 1843, 2301
TseFI GTSAC 4 cut(s) 314, 1340, 1474, 1822
TseI GCWGC 2 cut(s) 1628, 1634
Tsp45I GTSAC 4 cut(s) 314, 1340, 1474, 1822
TspDTI ATGAA 8 cut(s) 20, 52, 336, 569, 1536, 1667, 2015, 2127
TspGWI ACGGA 1 cut(s) 302
TspRI CASTG 7 cut(s) 481, 668, 1345, 1621, 1648, 1843, 2301
Tth111I GACNNNGTC 1 cut(s) 1097
Van91I CCANNNNNTGG 1 cut(s) 1750
VpaK11BI GGWCC 5 cut(s) 338, 755, 1235, 1380, 1553
XagI CCTNNNNNAGG 3 cut(s) 1008, 1410, 1509
XapI RAATTY 3 cut(s) 1432, 1654, 1966
XbaI TCTAGA 1 cut(s) 1807
XceI RCATGY 1 cut(s) 1316
XhoI CTCGAG 1 cut(s) 627
XmiI GTMKAC 1 cut(s) 240
XmnI GAANNNNTTC 2 cut(s) 106, 2226
XspI CTAG 8 cut(s) 782, 855, 914, 1167, 1374, 1443, 1482, 1808
ZrmI AGTACT 1 cut(s) 968
Zsp2I ATGCAT 3 cut(s) 1273, 1789, 2112
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.