Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0008270

Physical Location & Seq

Genomic Coordinates Forward (+)

29570447 .. 29574229

Gene Structure 3783 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1215300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1515 bp

ATGAGGCTATGCCTCGTCCTGGTTTTGATAGCCAGGCTCTTCGCAAACGCGGAATCTCTTGGCAGAGCTGATTTCCCCGACGGGTTTGTTTTCGGAACAGCTTCTTCAGCACACCAGTTTGAGGGCGCTGCAAATGAAGGAAACAAGGGAGTTAGCGTATGGGACACTTTCGCGAAAGAACCAGGGAGGATTCTGGACTTCAGCAATGCAGACACAGCAGTTGATCAATATCACAGATTTCAGAGTGATATAGAGTTGATGAAGGATATGGGAGTGGATGCATACAGATTCTCAATATCGTGGCCAAGAATTTTTCCAAACGGAAGAGGAGAATCCAACTCAGAAGGAATAAACTACTACAACAGCCTTATAGATGCTTTACTCGAAAAAGGGATCAAACCTTTTGTAACTCTATATCACTGGGATCTTCCACAAGCGCTTGAAGACAGATACGAAGGGTGGTTGAGTGCAAACATAATAAAAGACTTTGAACATTATGCCTTCACCTGCTTCAAAACCTTTGGTGACAGAGTTAAGAATTGGATCACCTTCAACGAGCCTCATGGTAATGCACTCCAAGGTTATGACTTGGGCATACAAGCTCCGGGGAGATGTTCTATTCTTGGCCATTTGTTTTGTAAGAAGGGAAATTCATCTGCTGAACCGTACATTGTTGCTCACAACATCCTCCTGTCTCATGCTGCCGCTTATCATAGTTACCGGCAATTTTTCAAGGAGACACAAGGAGGCCAAGTAGGAATTTCATTGGATGCCAAATGGTATGAGCCTGTATCTGATAATGATGAGGACAGAGATGCAGCACACAGAGCAATCGATTTTTCATTGGGATGGTTCCTTGATCCACTATTTTTTGGCGAATATCCACTTTCAATGAAGAAGCTTGTGGATAAGAGATTACCAGAGATTTCGCAGGAAATCTCAAATTTCCTGGTTGGAACCTTGGACTTTGTTGGCCTAAACCACTACACCACATATTATGCTCGAAATGACAGGACTCGGATTCGAAAGTTCATAATGCAGGATGCTACATCTGATGCTGCAGTCATTTCAACCCCATACAAAGGAGGAGTGGCAATCGGAGAAAGAGCAGCTTCCCGCTGGTTGCGCATAGTCCCATGGGGCATCCGAAAGTTGGCAAGACACCTGAAAGATAAGTATGGGAACCCCCCGATGATAATAACAGAAAATGGTATGGATGATCCAAATAAACGTTTCATACCTTTAGAAAAGGCTTTAAACGACGAAAAGAGGATAACCTTTCACAGGGACTATCTCTCGAACCTATCTGCTGCTATAAGGCAAGACAACTGTGACGTGCGTGGTTATTTTGTGTGGTCACTGCTCGACAACTGGGAATGGAACATGGGATACACTGTTCGGTTCGGTCTCTACTATGTGGATTACAAGAAGAACCTGACAAGGATTCCTAAAACTTCAGTACAGTGGTTTAGAACACTTCTCCAAGTTAACGATGACCCGAGACGCCACATCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005618 GO:0005623 GO:0008150 GO:0008152 GO:0008422 GO:0009505 GO:0015926 GO:0016787 GO:0016798 GO:0030312 GO:0044464 GO:0071704 GO:0071944 GO:1901135 GO:1901657

KEGG Pathways

Metabolic & Signaling

ko00460 ko00500 ko00940 ko01100 ko01110 map00460 map00500 map00940 map01100 map01110

Pfam Domains

Protein Families

Glyco_hydro_1

Protein Analysis

505

Amino Acids

57.91

Weight (kDa)

6.71

Isoelectric Point (pI)

27.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_1	PF00232	23 - 497	1.4e-156	Glycosyl hydrolase family 1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008270)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G54570 AT5G54570 AT5G54570
fragaria_vesca	FvH4_3g21512 FvH4_3g21512 FvH4_3g21512 FvH4_3g21512
malus_domestica	MD03G1215300.v1.1
prunus_persica	Prupe.4G197500_v2.0.a1 Prupe.4G197500_v2.0.a1 Prupe.4G197500_v2.0.a1 Prupe.4G197500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0036981
rosa_laevigata	RLG00000033740
rosa_multiflora	Rmu_co8347255.1_g000001 Rmu_sc0009301.1_g000001 Rmu_sc0013351.1_g000002
rosa_roxburghii	Rroxscaffold_1G00044000
rosa_rugosa	Rorug05G0159500
rosa_samantha	Rh5AG251600 Rh5BG253100 Rh5DG261700
rosa_wichuraiana	Rw5G023170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	515
Acc16I	TGCGCA	1 cut(s)	1127
Acc36I	ACCTGC	1 cut(s)	515
AccII	CGCG	2 cut(s)	50, 173
AciI	CCGC	3 cut(s)	50, 705, 1117
AclI	AACGTT	1 cut(s)	1231
AclWI	GGATC	5 cut(s)	401, 432, 551, 854, 1214
AcoI	YGGCCR	2 cut(s)	302, 625
AcsI	RAATTY	4 cut(s)	309, 649, 759, 943
AcuI	CTGAAG	3 cut(s)	90, 184, 1440
AcyI	GRCGYC	1 cut(s)	1504
AfaI	GTAC	2 cut(s)	668, 1461
AfeI	AGCGCT	1 cut(s)	438
AfiI	CCNNNNNNNGG	5 cut(s)	19, 121, 1082, 1153, 1284
AgsI	TTSAA	7 cut(s)	443, 491, 514, 553, 733, 891, 1071
AjiI	CACGTC	1 cut(s)	1336
AjnI	CCWGG	4 cut(s)	18, 32, 181, 948
AloI	GAACNNNNNNTCC	2 cut(s)	1380, 1412
AluBI	AGCT	5 cut(s)	68, 101, 602, 901, 1112
AluI	AGCT	5 cut(s)	68, 101, 602, 901, 1112
Alw26I	GTCTC	4 cut(s)	699, 731, 1412, 1495
AlwI	GGATC	5 cut(s)	401, 432, 551, 854, 1214
Ama87I	CYCGRG	1 cut(s)	1498
Aor51HI	AGCGCT	1 cut(s)	438
AoxI	GGCC	4 cut(s)	302, 625, 748, 973
ApeKI	GCWGC	6 cut(s)	128, 701, 818, 1058, 1109, 1310
ApoI	RAATTY	4 cut(s)	309, 649, 759, 943
Asp700I	GAANNNNTTC	1 cut(s)	100
AspLEI	GCGC	3 cut(s)	128, 439, 1128
AsuC2I	CCSGG	1 cut(s)	606
AsuHPI	GGTGA	3 cut(s)	496, 536, 538
AsuII	TTCGAA	1 cut(s)	1024
AvaI	CYCGRG	1 cut(s)	1498
BalI	TGGCCA	2 cut(s)	304, 627
BbsI	GAAGAC	1 cut(s)	450
BbvI	GCAGC	6 cut(s)	115, 688, 830, 1045, 1121, 1297
BccI	CCATC	1 cut(s)	843
BciT130I	CCWGG	4 cut(s)	20, 34, 183, 950
BciVI	GTATCC	1 cut(s)	1382
BclI	TGATCA	1 cut(s)	223
BcnI	CCSGG	1 cut(s)	606
BcoDI	GTCTC	4 cut(s)	699, 731, 1412, 1495
BfmI	CTRYAG	1 cut(s)	1059
BfoI	RGCGCY	2 cut(s)	129, 440
BfuAI	ACCTGC	1 cut(s)	515
BfuI	GTATCC	1 cut(s)	1382
BisI	GCNGC	7 cut(s)	129, 702, 705, 819, 1059, 1110, 1311
BlsI	GCNGC	7 cut(s)	130, 703, 706, 820, 1060, 1111, 1312
Bme1390I	CCNGG	5 cut(s)	20, 34, 183, 606, 950
BmeT110I	CYCGRG	1 cut(s)	1498
BmgBI	CACGTC	1 cut(s)	1336
BmiI	GGNNCC	3 cut(s)	854, 958, 1184
BmrFI	CCNGG	5 cut(s)	20, 34, 183, 606, 950
BmrI	ACTGGG	2 cut(s)	430, 1381
BmsI	GCATC	7 cut(s)	268, 364, 760, 805, 1033, 1045, 1152
BmuI	ACTGGG	2 cut(s)	430, 1381
BpiI	GAAGAC	1 cut(s)	450
Bpu14I	TTCGAA	1 cut(s)	1024
BpuMI	CCSGG	1 cut(s)	606
Bsa29I	ATCGAT	1 cut(s)	834
BsaBI	GATNNNNATC	1 cut(s)	228
BsaHI	GRCGYC	1 cut(s)	1504
BsaI	GGTCTC	1 cut(s)	1412
BsaJI	CCNNGG	5 cut(s)	182, 577, 605, 960, 1136
BsaXI	ACNNNNNCTCC	4 cut(s)	141, 171, 738, 768
Bsc4I	CCNNNNNNNGG	5 cut(s)	19, 121, 1082, 1153, 1284
Bse118I	RCCGGY	1 cut(s)	720
Bse1I	ACTGG	3 cut(s)	115, 425, 1376
Bse3DI	GCAATG	1 cut(s)	211
Bse8I	GATNNNNATC	1 cut(s)	228
BseBI	CCWGG	4 cut(s)	20, 34, 183, 950
BseCI	ATCGAT	1 cut(s)	834
BseDI	CCNNGG	5 cut(s)	182, 577, 605, 960, 1136
BseGI	GGATG	7 cut(s)	283, 684, 775, 854, 1048, 1143, 1222
BseJI	GATNNNNATC	1 cut(s)	228
BseLI	CCNNNNNNNGG	5 cut(s)	19, 121, 1082, 1153, 1284
BseMI	GCAATG	1 cut(s)	211
BseMII	CTCAG	1 cut(s)	354
BseNI	ACTGG	3 cut(s)	115, 425, 1376
BseRI	GAGGAG	2 cut(s)	342, 1101
BseXI	GCAGC	6 cut(s)	115, 688, 830, 1045, 1121, 1297
Bsh1236I	CGCG	2 cut(s)	50, 173
BshFI	GGCC	4 cut(s)	304, 627, 750, 975
BshVI	ATCGAT	1 cut(s)	834
BsiHKCI	CYCGRG	1 cut(s)	1498
BsiSI	CCGG	2 cut(s)	605, 721
BslFI	GGGAC	3 cut(s)	176, 1118, 1301
BslI	CCNNNNNNNGG	5 cut(s)	19, 121, 1082, 1153, 1284
BsmAI	GTCTC	4 cut(s)	699, 731, 1412, 1495
BsmBI	CGTCTC	1 cut(s)	1495
BsmFI	GGGAC	3 cut(s)	176, 1118, 1301
BsnI	GGCC	4 cut(s)	304, 627, 750, 975
Bso31I	GGTCTC	1 cut(s)	1412
BsoBI	CYCGRG	1 cut(s)	1498
Bsp119I	TTCGAA	1 cut(s)	1024
Bsp143I	GATC	6 cut(s)	223, 393, 424, 543, 859, 1219
Bsp19I	CCATGG	1 cut(s)	1136
Bsp68I	TCGCGA	1 cut(s)	173
BspACI	CCGC	3 cut(s)	50, 705, 1117
BspANI	GGCC	4 cut(s)	304, 627, 750, 975
BspCNI	CTCAG	1 cut(s)	353
BspDI	ATCGAT	1 cut(s)	834
BspFNI	CGCG	2 cut(s)	50, 173
BspLI	GGNNCC	3 cut(s)	854, 958, 1184
BspMAI	CTGCAG	1 cut(s)	1063
BspMI	ACCTGC	1 cut(s)	515
BspPI	GGATC	5 cut(s)	401, 432, 551, 854, 1214
BspQI	GCTCTTC	1 cut(s)	44
BspT104I	TTCGAA	1 cut(s)	1024
BspTNI	GGTCTC	1 cut(s)	1412
BsrDI	GCAATG	1 cut(s)	211
BsrFI	RCCGGY	1 cut(s)	720
BsrI	ACTGG	3 cut(s)	115, 425, 1376
BssAI	RCCGGY	1 cut(s)	720
BssECI	CCNNGG	5 cut(s)	182, 577, 605, 960, 1136
BssMI	GATC	6 cut(s)	223, 393, 424, 543, 859, 1219
BssNI	GRCGYC	1 cut(s)	1504
BssT1I	CCWWGG	3 cut(s)	577, 960, 1136
Bst2UI	CCWGG	4 cut(s)	20, 34, 183, 950
Bst4CI	ACNGT	4 cut(s)	666, 1331, 1396, 1464
Bst6I	CTCTTC	2 cut(s)	44, 319
BstACI	GRCGYC	1 cut(s)	1504
BstBI	TTCGAA	1 cut(s)	1024
BstDEI	CTNAG	1 cut(s)	340
BstDSI	CCRYGG	1 cut(s)	1136
BstENI	CCTNNNNNAGG	1 cut(s)	1282
BstF5I	GGATG	7 cut(s)	283, 684, 775, 854, 1048, 1143, 1222
BstFNI	CGCG	2 cut(s)	50, 173
BstH2I	RGCGCY	2 cut(s)	129, 440
BstHHI	GCGC	3 cut(s)	128, 439, 1128
BstKTI	GATC	6 cut(s)	226, 396, 427, 546, 862, 1222
BstMAI	GTCTC	4 cut(s)	699, 731, 1412, 1495
BstMBI	GATC	6 cut(s)	223, 393, 424, 543, 859, 1219
BstMWI	GCNNNNNNNGC	4 cut(s)	107, 215, 827, 1125
BstNI	CCWGG	4 cut(s)	20, 34, 183, 950
BstSCI	CCNGG	5 cut(s)	18, 32, 181, 604, 948
BstSFI	CTRYAG	1 cut(s)	1059
BstUI	CGCG	2 cut(s)	50, 173
BstV1I	GCAGC	6 cut(s)	115, 688, 830, 1045, 1121, 1297
BstV2I	GAAGAC	1 cut(s)	450
BstX2I	RGATCY	1 cut(s)	424
BstYI	RGATCY	1 cut(s)	424
Bsu15I	ATCGAT	1 cut(s)	834
BsuI	GTATCC	1 cut(s)	1382
BsuRI	GGCC	4 cut(s)	304, 627, 750, 975
BsuTUI	ATCGAT	1 cut(s)	834
BtgI	CCRYGG	1 cut(s)	1136
BtrI	CACGTC	1 cut(s)	1336
BtsCI	GGATG	7 cut(s)	283, 684, 775, 854, 1048, 1143, 1222
BtsI	GCAGTG	1 cut(s)	1358
BtsIMutI	CAGTG	4 cut(s)	418, 1358, 1392, 1469
BtuMI	TCGCGA	1 cut(s)	173
BveI	ACCTGC	1 cut(s)	515
CfoI	GCGC	3 cut(s)	128, 439, 1128
Cfr10I	RCCGGY	1 cut(s)	720
ClaI	ATCGAT	1 cut(s)	834
Csp6I	GTAC	2 cut(s)	667, 1460
CviAII	CATG	4 cut(s)	563, 698, 1137, 1384
CviQI	GTAC	2 cut(s)	667, 1460
DdeI	CTNAG	1 cut(s)	340
DpnI	GATC	6 cut(s)	225, 395, 426, 545, 861, 1221
DpnII	GATC	6 cut(s)	223, 393, 424, 543, 859, 1219
DraI	TTTAAA	1 cut(s)	1257
EaeI	YGGCCR	2 cut(s)	302, 625
Eam1104I	CTCTTC	2 cut(s)	44, 319
EarI	CTCTTC	2 cut(s)	44, 319
Eco130I	CCWWGG	3 cut(s)	577, 960, 1136
Eco31I	GGTCTC	1 cut(s)	1412
Eco47III	AGCGCT	1 cut(s)	438
Eco57I	CTGAAG	3 cut(s)	90, 184, 1440
Eco88I	CYCGRG	1 cut(s)	1498
EcoNI	CCTNNNNNAGG	1 cut(s)	1282
EcoRII	CCWGG	4 cut(s)	18, 32, 181, 948
EcoT14I	CCWWGG	3 cut(s)	577, 960, 1136
EcoT22I	ATGCAT	1 cut(s)	283
ErhI	CCWWGG	3 cut(s)	577, 960, 1136
Esp3I	CGTCTC	1 cut(s)	1495
FaeI	CATG	4 cut(s)	566, 701, 1140, 1387
FalI	AAGNNNNNCTT	2 cut(s)	1096, 1128
FaqI	GGGAC	3 cut(s)	176, 1118, 1301
FatI	CATG	4 cut(s)	562, 697, 1136, 1383
FauI	CCCGC	1 cut(s)	1124
FbaI	TGATCA	1 cut(s)	223
Fnu4HI	GCNGC	7 cut(s)	129, 702, 705, 819, 1059, 1110, 1311
FokI	GGATG	7 cut(s)	290, 671, 782, 861, 1055, 1130, 1229
Fsp4HI	GCNGC	7 cut(s)	129, 702, 705, 819, 1059, 1110, 1311
FspI	TGCGCA	1 cut(s)	1127
GlaI	GCGC	3 cut(s)	127, 438, 1127
GluI	GCNGC	7 cut(s)	129, 702, 705, 819, 1059, 1110, 1311
HaeII	RGCGCY	2 cut(s)	129, 440
HaeIII	GGCC	4 cut(s)	304, 627, 750, 975
HapII	CCGG	2 cut(s)	605, 721
HhaI	GCGC	3 cut(s)	128, 439, 1128
Hin1I	GRCGYC	1 cut(s)	1504
Hin1II	CATG	4 cut(s)	566, 701, 1140, 1387
Hin6I	GCGC	3 cut(s)	126, 437, 1126
HinP1I	GCGC	3 cut(s)	126, 437, 1126
HincII	GTYRAC	1 cut(s)	1489
HindII	GTYRAC	1 cut(s)	1489
HindIII	AAGCTT	1 cut(s)	899
HinfI	GANTC	7 cut(s)	53, 190, 288, 332, 1015, 1021, 1444
HpaI	GTTAAC	1 cut(s)	1489
HpaII	CCGG	2 cut(s)	605, 721
HphI	GGTGA	3 cut(s)	496, 536, 538
Hpy166II	GTNNAC	1 cut(s)	1489
Hpy188I	TCNGA	8 cut(s)	95, 243, 343, 796, 1020, 1054, 1100, 1148
Hpy188III	TCNNGA	3 cut(s)	172, 194, 1297
Hpy8I	GTNNAC	1 cut(s)	1489
Hpy99I	CGWCG	2 cut(s)	83, 1265
HpyAV	CCTTC	7 cut(s)	131, 256, 338, 449, 511, 559, 637
HpyCH4III	ACNGT	4 cut(s)	666, 1331, 1396, 1464
HpyCH4IV	ACGT	2 cut(s)	1231, 1335
HpyCH4V	TGCA	8 cut(s)	131, 209, 281, 470, 572, 818, 1039, 1061
HpyF10VI	GCNNNNNNNGC	4 cut(s)	107, 215, 827, 1125
HpyF3I	CTNAG	1 cut(s)	340
HpySE526I	ACGT	2 cut(s)	1231, 1335
Hsp92I	GRCGYC	1 cut(s)	1504
Hsp92II	CATG	4 cut(s)	566, 701, 1140, 1387
HspAI	GCGC	3 cut(s)	126, 437, 1126
Ksp22I	TGATCA	1 cut(s)	223
KspAI	GTTAAC	1 cut(s)	1489
Kzo9I	GATC	6 cut(s)	223, 393, 424, 543, 859, 1219
LguI	GCTCTTC	1 cut(s)	44
LmnI	GCTCC	1 cut(s)	607
Lsp1109I	GCAGC	6 cut(s)	115, 688, 830, 1045, 1121, 1297
LweI	GCATC	7 cut(s)	268, 364, 760, 805, 1033, 1045, 1152
MaeII	ACGT	2 cut(s)	1231, 1335
MaeIII	GTNAC	5 cut(s)	406, 524, 716, 1331, 1356
MalI	GATC	6 cut(s)	225, 395, 426, 545, 861, 1221
MboI	GATC	6 cut(s)	223, 393, 424, 543, 859, 1219
MboII	GAAGA	7 cut(s)	31, 96, 336, 419, 455, 907, 1441
MflI	RGATCY	1 cut(s)	424
MlsI	TGGCCA	2 cut(s)	304, 627
MluCI	AATT	6 cut(s)	309, 538, 649, 725, 759, 943
MluNI	TGGCCA	2 cut(s)	304, 627
MlyI	GAGTC	1 cut(s)	1009
MmeI	TCCRAC	2 cut(s)	360, 934
Mox20I	TGGCCA	2 cut(s)	304, 627
Mph1103I	ATGCAT	1 cut(s)	283
MroXI	GAANNNNTTC	1 cut(s)	100
MscI	TGGCCA	2 cut(s)	304, 627
MseI	TTAA	3 cut(s)	534, 1256, 1488
MslI	CAYNNNNRTG	2 cut(s)	567, 847
Msp20I	TGGCCA	2 cut(s)	304, 627
MspA1I	CMGCKG	1 cut(s)	1119
MspI	CCGG	2 cut(s)	605, 721
MspR9I	CCNGG	5 cut(s)	20, 34, 183, 606, 950
MvaI	CCWGG	4 cut(s)	20, 34, 183, 950
MvnI	CGCG	2 cut(s)	50, 173
MwoI	GCNNNNNNNGC	4 cut(s)	107, 215, 827, 1125
NciI	CCSGG	1 cut(s)	606
NcoI	CCATGG	1 cut(s)	1136
NdeII	GATC	6 cut(s)	223, 393, 424, 543, 859, 1219
NlaIII	CATG	4 cut(s)	566, 701, 1140, 1387
NlaIV	GGNNCC	3 cut(s)	854, 958, 1184
NmuCI	GTSAC	3 cut(s)	524, 1331, 1356
NruI	TCGCGA	1 cut(s)	173
NsbI	TGCGCA	1 cut(s)	1127
NsiI	ATGCAT	1 cut(s)	283
NspV	TTCGAA	1 cut(s)	1024
PaqCI	CACCTGC	1 cut(s)	515
PciSI	GCTCTTC	1 cut(s)	44
PdmI	GAANNNNTTC	1 cut(s)	100
PfeI	GAWTC	6 cut(s)	53, 190, 288, 332, 1021, 1444
PkrI	GCNGC	7 cut(s)	130, 703, 706, 820, 1060, 1111, 1312
PleI	GAGTC	1 cut(s)	1009
PpsI	GAGTC	1 cut(s)	1009
Psp1406I	AACGTT	1 cut(s)	1231
Psp6I	CCWGG	4 cut(s)	18, 32, 181, 948
PspGI	CCWGG	4 cut(s)	18, 32, 181, 948
PspN4I	GGNNCC	3 cut(s)	854, 958, 1184
PsrI	GAACNNNNNNTAC	2 cut(s)	1373, 1405
PstI	CTGCAG	1 cut(s)	1063
PsuI	RGATCY	1 cut(s)	424
RruI	TCGCGA	1 cut(s)	173
RsaI	GTAC	2 cut(s)	668, 1461
RsaNI	GTAC	2 cut(s)	667, 1460
RseI	CAYNNNNRTG	2 cut(s)	567, 847
SapI	GCTCTTC	1 cut(s)	44
SaqAI	TTAA	3 cut(s)	534, 1256, 1488
SatI	GCNGC	7 cut(s)	129, 702, 705, 819, 1059, 1110, 1311
Sau3AI	GATC	6 cut(s)	223, 393, 424, 543, 859, 1219
SchI	GAGTC	1 cut(s)	1009
ScrFI	CCNGG	5 cut(s)	20, 34, 183, 606, 950
SfaNI	GCATC	7 cut(s)	268, 364, 760, 805, 1033, 1045, 1152
SfcI	CTRYAG	1 cut(s)	1059
SfuI	TTCGAA	1 cut(s)	1024
SmiMI	CAYNNNNRTG	2 cut(s)	567, 847
Sse9I	AATT	6 cut(s)	309, 538, 649, 725, 759, 943
SsiI	CCGC	3 cut(s)	50, 705, 1117
StyD4I	CCNGG	5 cut(s)	18, 32, 181, 604, 948
StyI	CCWWGG	3 cut(s)	577, 960, 1136
TaaI	ACNGT	4 cut(s)	666, 1331, 1396, 1464
TaiI	ACGT	2 cut(s)	1234, 1338
TaqI	TCGA	6 cut(s)	384, 834, 1003, 1024, 1298, 1365
TaqII	GACCGA	1 cut(s)	1394
TasI	AATT	6 cut(s)	309, 538, 649, 725, 759, 943
TatI	WGTACW	1 cut(s)	1459
TauI	GCSGC	1 cut(s)	707
TfiI	GAWTC	6 cut(s)	53, 190, 288, 332, 1021, 1444
Tru1I	TTAA	3 cut(s)	534, 1256, 1488
Tru9I	TTAA	3 cut(s)	534, 1256, 1488
TscAI	CASTG	4 cut(s)	425, 1365, 1399, 1469
TseFI	GTSAC	3 cut(s)	524, 1331, 1356
TseI	GCWGC	6 cut(s)	128, 701, 818, 1058, 1109, 1310
Tsp45I	GTSAC	3 cut(s)	524, 1331, 1356
TspDTI	ATGAA	8 cut(s)	150, 275, 642, 753, 831, 908, 1021, 1225
TspGWI	ACGGA	1 cut(s)	336
TspRI	CASTG	4 cut(s)	425, 1365, 1399, 1469
XagI	CCTNNNNNAGG	1 cut(s)	1282
XapI	RAATTY	4 cut(s)	309, 649, 759, 943
XmnI	GAANNNNTTC	1 cut(s)	100
Zsp2I	ATGCAT	1 cut(s)	283

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.