Rorug05G0159500

Belongs to the glycosyl hydrolase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0008270
Physical Location & Seq
Reverse (-)
14875327 .. 14878430
3104 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0159500.1

Sequence Viewer

Length: 1707 bp
ATGCACCGGCAGTCTGTACCTTTCAACAGCTTCTCCATTCTCTTTACTCTAAAATCATGTACCCAATTGCATAACCACGCAACCATTCAACACCTACATTCTCATATTGTTAAACTCGGGTTTGATTCTCACGTATACGTGGCCACCTCACTTCTCCATGCTTACGTCGTGGTCTCGTTTGTGGATGCATGTAAACTGTTCAAGGAAATGCCTGAGAAGAGCACAGTCACCTGGAACACAATGATTTCTGGGTATTCGAGGACCGGGAATGTAGAGAGAGCAAGACTGGTGTTTGAGGAAATGCCACTGAGAGATGTTTCATCATGGTGTGCAATGGTCACAGCTTATGTGAACAATGGCAAACATGAGCTTGGGTCATCACTCTTTCGGTATATGGTAATGGGTGAAGTTCATGGGTTTATGGTCAAGAACCGGTGGGAGATGAATGCGGAGCTCGGTACAGTTTTGATCGGCATGTATGCGAAATGTGGGTTTTTGAAGGGAGCAGAACAGGTTTTTGAGTTGATGCAAGAGAAGAATGTGATGGCTTGGACTTCATTGATTTGTGGATCGGCACAACATGGATACAGCAAAGAAGCATTGTGTTTGTTTGAGATGATGCAAAACAGCGGTGTAAGACCTAATGAACTGACTTTTACAGGAGTTATCAGTGCTTGTGTGAACACGGGACTAGTTGAGGAGGGTTGCAAATATTTCAATTTGATTGAAGAAAGTGGCTTGGAGCTTCGGATTCAGCATTATGGATGCATGGTCGATTTGTATGGCAAGGCGGGGCTGTTAGAGGAAGCCTATGATTTTATTAAGAACATGAGACTTGAACCCAATATTGCTGTCTGGGGAGCTTTTTTGTCGGCTTGCAAGGAGCATAAACAGTTTGAGATGGCCGAAAGAGTTGTTGAGGAGGTCATGAAGATGGTGAAACCAGAGAATGATAGTAGGGTTTACTCTCTTATCGCCGATTTGTATGTTTTGGGTGGAAAGTGGGATGATGCTGAAAGAGTGAGGAAATTAATGGTGAACCACAAGGCGAAAAATGATAGTGAGGCACCTCAGTGGCAGCAGCCCAGTAGCTTTATCGAGATCGGAAGCGTATTGTTCAAAGGCTCTAATTCTGCAATTGGCAGAAGGCTGTTTACAAACAGGAACAACACAATCAGAATTACAACCTTAGTTCTGCAGCTAAATAATGAGAAGCCAAGTTTATTAAGGAGAAGAGAAGCAATTGGTTTTGGCTTCTACTTTGGCCTTGTTGAAGTGCTCTTGCCAGGTCAACCCACTGAAGCAGTTGAAGTTGCTCCTTGTGAATTTACTGTAGCTCCTTCTGGACTAGCTTTCTGTGACAAAGTAGTGGGATATGGAGCTGAGGCTGTTAAGGGACAGATGATTAAGGCACACTACACTGAGTACACTGGGAAATTGGAGAATGGCAAGGTGTTCGATAGTAGCTATAATCGCGGGAAGCCCCTTACATTCCGAATTGGTGCTGGTGAGGTAATCAAAGGGTGGGACCAAGGTATTCTTGGTGGCGATGGAATTCCTCCCATGCTTGCTGGGGGAAAGCGCACCTTGAAGATCCCTGCAGAACTTGCATATGGTGCACGTGGAGCTGGGTGTAGAGGAGGTTCGTGTATTATTCCTCCATATTCAGTTCTTTTGTTTGATGTGGAATTCATTGGCAAGGCATGA

Protein Analysis

568

Amino Acids

63.24

Weight (kDa)

7.54

Isoelectric Point (pI)

38.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 74 - 106 2.2e-07 PPR repeat family
PPR_1 PF12854 75 - 101 1.5e-06 PPR repeat
PPR PF01535 76 - 104 1.4e-08 PPR repeat
PPR_2 PF13041 179 - 228 5.7e-11 PPR repeat family
FKBP_C PF00254 463 - 564 7.3e-26 FKBP-type peptidyl-prolyl cis-trans isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1066
AccI GTMKAC 1 cut(s) 135
AccII CGCG 1 cut(s) 1476
AciI CCGC 4 cut(s) 449, 630, 791, 1476
AclWI GGATC 2 cut(s) 577, 1588
AcoI YGGCCR 2 cut(s) 141, 903
AcsI RAATTY 3 cut(s) 1325, 1554, 1688
AcuI CTGAAG 1 cut(s) 1320
AcvI CACGTG 1 cut(s) 1622
AfaI GTAC 4 cut(s) 18, 61, 460, 1427
AgeI ACCGGT 1 cut(s) 432
AhlI ACTAGT 1 cut(s) 691
AjnI CCWGG 2 cut(s) 230, 1285
AleI CACNNNNGTG 1 cut(s) 1072
Alw21I GWGCWC 4 cut(s) 224, 456, 1281, 1621
Alw26I GTCTC 2 cut(s) 178, 826
Alw44I GTGCAC 1 cut(s) 1617
AlwI GGATC 2 cut(s) 577, 1588
Ama87I CYCGRG 1 cut(s) 116
AoxI GGCC 3 cut(s) 141, 903, 1264
ApaLI GTGCAC 1 cut(s) 1617
ApeKI GCWGC 3 cut(s) 1078, 1081, 1198
ApoI RAATTY 3 cut(s) 1325, 1554, 1688
AseI ATTAAT 1 cut(s) 1031
AsiGI ACCGGT 1 cut(s) 432
AspLEI GCGC 1 cut(s) 1584
AspS9I GGNCC 2 cut(s) 261, 1528
AsuC2I CCSGG 1 cut(s) 265
AsuHPI GGTGA 5 cut(s) 220, 416, 949, 1048, 1520
AvaI CYCGRG 1 cut(s) 116
AvaII GGWCC 2 cut(s) 261, 1528
BaeGI GKGCMC 1 cut(s) 1621
BalI TGGCCA 1 cut(s) 143
BanI GGYRCC 1 cut(s) 1066
BanII GRGCYC 1 cut(s) 456
BarI GAAGNNNNNNTAC 2 cut(s) 1138, 1170
BbrPI CACGTG 1 cut(s) 1622
Bbv12I GWGCWC 4 cut(s) 224, 456, 1281, 1621
BbvCI CCTCAGC 1 cut(s) 1383
BbvI GCAGC 3 cut(s) 1090, 1093, 1210
BccI CCATC 4 cut(s) 538, 895, 928, 1544
BcgI CGANNNNNNTGC 2 cut(s) 1438, 1472
BciT130I CCWGG 2 cut(s) 232, 1287
BciVI GTATCC 1 cut(s) 578
BcnI CCSGG 1 cut(s) 265
BcoDI GTCTC 2 cut(s) 178, 826
BcuI ACTAGT 1 cut(s) 691
BfaI CTAG 2 cut(s) 692, 1349
BfmI CTRYAG 3 cut(s) 1196, 1332, 1599
BfuI GTATCC 1 cut(s) 578
BisI GCNGC 3 cut(s) 1079, 1082, 1199
BlsI GCNGC 3 cut(s) 1080, 1083, 1200
Bme1390I CCNGG 3 cut(s) 232, 265, 1287
Bme18I GGWCC 2 cut(s) 261, 1528
BmeT110I CYCGRG 1 cut(s) 116
BmgT120I GGNCC 2 cut(s) 261, 1528
BmiI GGNNCC 2 cut(s) 1068, 1529
BmrFI CCNGG 3 cut(s) 232, 265, 1287
BmrI ACTGGG 2 cut(s) 1080, 1440
BmsI GCATC 5 cut(s) 175, 516, 609, 755, 1000
BmuI ACTGGG 2 cut(s) 1080, 1440
Bpu10I CCTNAGC 1 cut(s) 1383
BpuMI CCSGG 1 cut(s) 265
BsaAI YACGTR 3 cut(s) 133, 139, 1622
BsaI GGTCTC 1 cut(s) 178
BsaJI CCNNGG 1 cut(s) 1531
BsaWI WCCGGW 1 cut(s) 432
BsaXI ACNNNNNCTCC 4 cut(s) 17, 47, 1321, 1351
Bse118I RCCGGY 2 cut(s) 6, 432
Bse1I ACTGG 3 cut(s) 291, 1086, 1435
Bse3DI GCAATG 1 cut(s) 339
BseBI CCWGG 2 cut(s) 232, 1287
BseDI CCNNGG 1 cut(s) 1531
BseGI GGATG 3 cut(s) 190, 770, 1012
BseMI GCAATG 1 cut(s) 339
BseMII CTCAG 5 cut(s) 204, 299, 1085, 1374, 1413
BseNI ACTGG 3 cut(s) 291, 1086, 1435
BseRI GAGGAG 3 cut(s) 713, 935, 1653
BseSI GKGCMC 1 cut(s) 1621
BseXI GCAGC 3 cut(s) 1090, 1093, 1210
BseYI CCCAGC 2 cut(s) 1571, 1628
Bsh1236I CGCG 1 cut(s) 1476
BshFI GGCC 3 cut(s) 143, 905, 1266
BshNI GGYRCC 1 cut(s) 1066
BshTI ACCGGT 1 cut(s) 432
BsiHKAI GWGCWC 4 cut(s) 224, 456, 1281, 1621
BsiHKCI CYCGRG 1 cut(s) 116
BsiSI CCGG 3 cut(s) 7, 264, 433
BslFI GGGAC 3 cut(s) 702, 1410, 1541
BsmAI GTCTC 2 cut(s) 178, 826
BsmFI GGGAC 3 cut(s) 702, 1410, 1541
BsmI GAATGC 1 cut(s) 451
BsnI GGCC 3 cut(s) 143, 905, 1266
Bso31I GGTCTC 1 cut(s) 178
BsoBI CYCGRG 1 cut(s) 116
Bsp1286I GDGCHC 4 cut(s) 224, 456, 1281, 1621
Bsp143I GATC 4 cut(s) 468, 569, 1101, 1593
BspACI CCGC 4 cut(s) 449, 630, 791, 1476
BspANI GGCC 3 cut(s) 143, 905, 1266
BspCNI CTCAG 5 cut(s) 205, 300, 1084, 1375, 1414
BspFNI CGCG 1 cut(s) 1476
BspHI TCATGA 1 cut(s) 927
BspLI GGNNCC 2 cut(s) 1068, 1529
BspMAI CTGCAG 2 cut(s) 1200, 1603
BspPI GGATC 2 cut(s) 577, 1588
BspQI GCTCTTC 1 cut(s) 212
BspT107I GGYRCC 1 cut(s) 1066
BspTNI GGTCTC 1 cut(s) 178
BsrDI GCAATG 1 cut(s) 339
BsrFI RCCGGY 2 cut(s) 6, 432
BsrI ACTGG 3 cut(s) 291, 1086, 1435
BssAI RCCGGY 2 cut(s) 6, 432
BssECI CCNNGG 1 cut(s) 1531
BssMI GATC 4 cut(s) 468, 569, 1101, 1593
BssNAI GTATAC 1 cut(s) 136
BssT1I CCWWGG 1 cut(s) 1531
Bst1107I GTATAC 1 cut(s) 136
Bst2UI CCWGG 2 cut(s) 232, 1287
Bst4CI ACNGT 5 cut(s) 198, 226, 463, 894, 1333
Bst6I CTCTTC 2 cut(s) 212, 1228
BstAPI GCANNNNNTGC 2 cut(s) 1607, 1616
BstBAI YACGTR 3 cut(s) 133, 139, 1622
BstC8I GCNNGC 2 cut(s) 877, 1569
BstDEI CTNAG 6 cut(s) 213, 308, 1071, 1189, 1383, 1422
BstF5I GGATG 3 cut(s) 190, 770, 1012
BstFNI CGCG 1 cut(s) 1476
BstHHI GCGC 1 cut(s) 1584
BstKTI GATC 4 cut(s) 471, 572, 1104, 1596
BstMAI GTCTC 2 cut(s) 178, 826
BstMBI GATC 4 cut(s) 468, 569, 1101, 1593
BstMWI GCNNNNNNNGC 4 cut(s) 1473, 1607, 1616, 1625
BstNI CCWGG 2 cut(s) 232, 1287
BstNSI RCATGY 2 cut(s) 192, 478
BstSCI CCNGG 3 cut(s) 230, 263, 1285
BstSFI CTRYAG 3 cut(s) 1196, 1332, 1599
BstSLI GKGCMC 1 cut(s) 1621
BstUI CGCG 1 cut(s) 1476
BstV1I GCAGC 3 cut(s) 1090, 1093, 1210
BstX2I RGATCY 1 cut(s) 1593
BstYI RGATCY 1 cut(s) 1593
BstZ17I GTATAC 1 cut(s) 136
BsuI GTATCC 1 cut(s) 578
BsuRI GGCC 3 cut(s) 143, 905, 1266
BtgZI GCGATG 1 cut(s) 1563
BtsCI GGATG 3 cut(s) 190, 770, 1012
BtsIMutI CAGTG 6 cut(s) 305, 676, 1079, 1296, 1419, 1428
Cac8I GCNNGC 2 cut(s) 877, 1569
CciI TCATGA 1 cut(s) 927
CfoI GCGC 1 cut(s) 1584
Cfr10I RCCGGY 2 cut(s) 6, 432
Cfr13I GGNCC 2 cut(s) 261, 1528
Csp6I GTAC 4 cut(s) 17, 60, 459, 1426
CspAI ACCGGT 1 cut(s) 432
CviQI GTAC 4 cut(s) 17, 60, 459, 1426
DdeI CTNAG 6 cut(s) 213, 308, 1071, 1189, 1383, 1422
DpnI GATC 4 cut(s) 470, 571, 1103, 1595
DpnII GATC 4 cut(s) 468, 569, 1101, 1593
EaeI YGGCCR 2 cut(s) 141, 903
Eam1104I CTCTTC 2 cut(s) 212, 1228
EarI CTCTTC 2 cut(s) 212, 1228
Ecl136II GAGCTC 1 cut(s) 454
Eco130I CCWWGG 1 cut(s) 1531
Eco24I GRGCYC 1 cut(s) 456
Eco31I GGTCTC 1 cut(s) 178
Eco47I GGWCC 2 cut(s) 261, 1528
Eco53kI GAGCTC 1 cut(s) 454
Eco57I CTGAAG 1 cut(s) 1320
Eco72I CACGTG 1 cut(s) 1622
Eco88I CYCGRG 1 cut(s) 116
EcoICRI GAGCTC 1 cut(s) 454
EcoRI GAATTC 2 cut(s) 1554, 1688
EcoRII CCWGG 2 cut(s) 230, 1285
EcoT14I CCWWGG 1 cut(s) 1531
EcoT22I ATGCAT 2 cut(s) 190, 770
EcoT38I GRGCYC 1 cut(s) 456
ErhI CCWWGG 1 cut(s) 1531
FalI AAGNNNNNCTT 4 cut(s) 1524, 1556, 1571, 1603
FaqI GGGAC 3 cut(s) 702, 1410, 1541
FauI CCCGC 2 cut(s) 784, 1469
FauNDI CATATG 1 cut(s) 1612
FblI GTMKAC 1 cut(s) 135
Fnu4HI GCNGC 3 cut(s) 1079, 1082, 1199
FokI GGATG 3 cut(s) 197, 777, 1019
FriOI GRGCYC 1 cut(s) 456
Fsp4HI GCNGC 3 cut(s) 1079, 1082, 1199
FspBI CTAG 2 cut(s) 692, 1349
GlaI GCGC 1 cut(s) 1583
GluI GCNGC 3 cut(s) 1079, 1082, 1199
GsaI CCCAGC 2 cut(s) 1575, 1632
HaeIII GGCC 3 cut(s) 143, 905, 1266
HapII CCGG 3 cut(s) 7, 264, 433
HhaI GCGC 1 cut(s) 1584
Hin6I GCGC 1 cut(s) 1582
HinP1I GCGC 1 cut(s) 1582
HincII GTYRAC 1 cut(s) 1292
HindII GTYRAC 1 cut(s) 1292
HinfI GANTC 2 cut(s) 125, 751
HpaII CCGG 3 cut(s) 7, 264, 433
HphI GGTGA 5 cut(s) 220, 416, 949, 1048, 1520
Hpy188I TCNGA 4 cut(s) 750, 1106, 1178, 1496
Hpy188III TCNNGA 4 cut(s) 427, 928, 1099, 1344
Hpy99I CGWCG 1 cut(s) 170
HpyAV CCTTC 3 cut(s) 493, 1140, 1350
HpyCH4III ACNGT 5 cut(s) 198, 226, 463, 894, 1333
HpyCH4IV ACGT 4 cut(s) 132, 138, 165, 1621
HpyF10VI GCNNNNNNNGC 4 cut(s) 1473, 1607, 1616, 1625
HpyF3I CTNAG 6 cut(s) 213, 308, 1071, 1189, 1383, 1422
HpySE526I ACGT 4 cut(s) 132, 138, 165, 1621
HspAI GCGC 1 cut(s) 1582
Kzo9I GATC 4 cut(s) 468, 569, 1101, 1593
LguI GCTCTTC 1 cut(s) 212
LmnI GCTCC 9 cut(s) 451, 503, 742, 860, 883, 1321, 1342, 1379, 1625
Lsp1109I GCAGC 3 cut(s) 1090, 1093, 1210
LweI GCATC 5 cut(s) 175, 516, 609, 755, 1000
MaeI CTAG 2 cut(s) 692, 1349
MaeII ACGT 4 cut(s) 132, 138, 165, 1621
MaeIII GTNAC 3 cut(s) 226, 337, 1358
MalI GATC 4 cut(s) 470, 571, 1103, 1595
MboI GATC 4 cut(s) 468, 569, 1101, 1593
MboII GAAGA 6 cut(s) 229, 547, 740, 943, 1245, 1603
MfeI CAATTG 3 cut(s) 65, 1137, 1242
MflI RGATCY 1 cut(s) 1593
MhlI GDGCHC 4 cut(s) 224, 456, 1281, 1621
MlsI TGGCCA 1 cut(s) 143
MluNI TGGCCA 1 cut(s) 143
Mox20I TGGCCA 1 cut(s) 143
Mph1103I ATGCAT 2 cut(s) 190, 770
MscI TGGCCA 1 cut(s) 143
MseI TTAA 6 cut(s) 111, 822, 1031, 1226, 1392, 1407
MslI CAYNNNNRTG 3 cut(s) 325, 932, 1072
Msp20I TGGCCA 1 cut(s) 143
MspA1I CMGCKG 1 cut(s) 630
MspI CCGG 3 cut(s) 7, 264, 433
MspR9I CCNGG 3 cut(s) 232, 265, 1287
MunI CAATTG 3 cut(s) 65, 1137, 1242
Mva1269I GAATGC 1 cut(s) 451
MvaI CCWGG 2 cut(s) 232, 1287
MvnI CGCG 1 cut(s) 1476
MwoI GCNNNNNNNGC 4 cut(s) 1473, 1607, 1616, 1625
NciI CCSGG 1 cut(s) 265
NdeI CATATG 1 cut(s) 1612
NdeII GATC 4 cut(s) 468, 569, 1101, 1593
NlaIV GGNNCC 2 cut(s) 1068, 1529
NmuCI GTSAC 3 cut(s) 226, 337, 1358
NsiI ATGCAT 2 cut(s) 190, 770
NspI RCATGY 2 cut(s) 192, 478
OliI CACNNNNGTG 1 cut(s) 1072
PagI TCATGA 1 cut(s) 927
PciSI GCTCTTC 1 cut(s) 212
PctI GAATGC 1 cut(s) 451
PfeI GAWTC 2 cut(s) 125, 751
PinAI ACCGGT 1 cut(s) 432
PkrI GCNGC 3 cut(s) 1080, 1083, 1200
PmaCI CACGTG 1 cut(s) 1622
PmlI CACGTG 1 cut(s) 1622
Ppu21I YACGTR 3 cut(s) 133, 139, 1622
PshBI ATTAAT 1 cut(s) 1031
Psp124BI GAGCTC 1 cut(s) 456
Psp6I CCWGG 2 cut(s) 230, 1285
PspCI CACGTG 1 cut(s) 1622
PspFI CCCAGC 2 cut(s) 1571, 1628
PspGI CCWGG 2 cut(s) 230, 1285
PspN4I GGNNCC 2 cut(s) 1068, 1529
PspPI GGNCC 2 cut(s) 261, 1528
PsrI GAACNNNNNNTAC 2 cut(s) 1627, 1659
PstI CTGCAG 2 cut(s) 1200, 1603
PsuI RGATCY 1 cut(s) 1593
RsaI GTAC 4 cut(s) 18, 61, 460, 1427
RsaNI GTAC 4 cut(s) 17, 60, 459, 1426
RseI CAYNNNNRTG 3 cut(s) 325, 932, 1072
SacI GAGCTC 1 cut(s) 456
SapI GCTCTTC 1 cut(s) 212
SaqAI TTAA 6 cut(s) 111, 822, 1031, 1226, 1392, 1407
SatI GCNGC 3 cut(s) 1079, 1082, 1199
Sau3AI GATC 4 cut(s) 468, 569, 1101, 1593
Sau96I GGNCC 2 cut(s) 261, 1528
ScrFI CCNGG 3 cut(s) 232, 265, 1287
SduI GDGCHC 4 cut(s) 224, 456, 1281, 1621
SfaNI GCATC 5 cut(s) 175, 516, 609, 755, 1000
SfcI CTRYAG 3 cut(s) 1196, 1332, 1599
SinI GGWCC 2 cut(s) 261, 1528
SmiMI CAYNNNNRTG 3 cut(s) 325, 932, 1072
SpeI ACTAGT 1 cut(s) 691
SsiI CCGC 4 cut(s) 449, 630, 791, 1476
SspI AATATT 2 cut(s) 713, 847
SspMI CTAG 2 cut(s) 692, 1349
SstI GAGCTC 1 cut(s) 456
StyD4I CCNGG 3 cut(s) 230, 263, 1285
StyI CCWWGG 1 cut(s) 1531
TaaI ACNGT 5 cut(s) 198, 226, 463, 894, 1333
TaiI ACGT 4 cut(s) 135, 141, 168, 1624
TaqI TCGA 4 cut(s) 257, 774, 1098, 1458
TatI WGTACW 1 cut(s) 1425
TfiI GAWTC 2 cut(s) 125, 751
Tru1I TTAA 6 cut(s) 111, 822, 1031, 1226, 1392, 1407
Tru9I TTAA 6 cut(s) 111, 822, 1031, 1226, 1392, 1407
TscAI CASTG 6 cut(s) 312, 676, 1079, 1303, 1426, 1435
TseFI GTSAC 3 cut(s) 226, 337, 1358
TseI GCWGC 3 cut(s) 1078, 1081, 1198
Tsp45I GTSAC 3 cut(s) 226, 337, 1358
TspDTI ATGAA 7 cut(s) 309, 401, 458, 546, 660, 944, 1681
TspRI CASTG 6 cut(s) 312, 676, 1079, 1303, 1426, 1435
VneI GTGCAC 1 cut(s) 1617
VpaK11BI GGWCC 2 cut(s) 261, 1528
VspI ATTAAT 1 cut(s) 1031
XapI RAATTY 3 cut(s) 1325, 1554, 1688
XceI RCATGY 2 cut(s) 192, 478
XcmI CCANNNNNNNNNTGG 1 cut(s) 1538
XmiI GTMKAC 1 cut(s) 135
XspI CTAG 2 cut(s) 692, 1349
Zsp2I ATGCAT 2 cut(s) 190, 770
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.