Rh5BG253100

Belongs to the glycosyl hydrolase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0008270
Physical Location & Seq
Reverse (-)
31470442 .. 31480746
10305 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG253100.1

Sequence Viewer

Length: 909 bp
ATGAGACTATGCATCGTCCTGTTTCTGATGACCAGACTCTTTACAAACTCTCAATCTCTTAGCAGGGCTGATTTCCCAGAAGGGTTTGTTTTCGGAACAGCTTCTTCGGCATACCAGGAGAAACAAGTAGGTCAGGTTGGAATTGCACTGGATGCAAAATGGCATGAACCTCTATCGGAAAGTGATGAGGACATAGATGCAGCACATAGAGCGAATGAATTCACATTGGGATGGTTCCTTGATCCACTATTTTTTGGCGACTATCCTCTCTCAATGAAGAAGCTTGTGGGTAAGCGATTACCTGAGATTTCGCAGGAAGAATCAAAATTCTTGGTGGGATCTGTGGACTTTATCGGCATAAACCACTACACCACATTATATGCTCGGAATGACAGGACACGGATTCGAAAATTTATATTGCAGGATGCTACATCTGATGCTGCAGTCATTACAACACAATACAAAGGAGGAGTGGCTATTGGAGAAAAAGCAGCTTCTCGCTGGTTACGCATAGTCCCATGGGGCATCCGAAAGTTGGCAAGATACATCAAAGATAAGTACGGGAACCCCCCAGTGATGATAACAGAAAATGGTATGGATGATCCAAATAAACCTTTCATATCTATGGAGAAGGCTTTAAATGATGAGAAGAGGATACGATTTCACAGGGACTATCTCTCAAACCTATCAGCTGCTATAAGGGAAGACAAATGCGATGTTCGTGGTTATTTTGTGTGGTCATTACTTGACAACTGGGAATGGAACATGGGCTACACTGTCAGATTTGGGCTCTACTATGTCGATTACAAGCAGAACCTTACGAGGATTCCTAAATCTTCTGTTCAATGGTTTACAAGATTTCTCCAAGTTAACAGTCATCAGAGTTTTGTGCAGACACAGCACATGTAA

Protein Analysis

302

Amino Acids

35.03

Weight (kDa)

8.87

Isoelectric Point (pI)

32.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_1 PF00232 42 - 291 6.1e-66 Glycosyl hydrolase family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 236, 346, 596
AcsI RAATTY 3 cut(s) 218, 326, 410
AfaI GTAC 1 cut(s) 560
AfiI CCNNNNNNNGG 1 cut(s) 535
AflIII ACRYGT 1 cut(s) 903
AgsI TTSAA 1 cut(s) 845
AjnI CCWGG 1 cut(s) 114
AluBI AGCT 4 cut(s) 101, 283, 494, 692
AluI AGCT 4 cut(s) 101, 283, 494, 692
AlwI GGATC 3 cut(s) 236, 346, 596
ApeKI GCWGC 4 cut(s) 200, 440, 491, 692
ApoI RAATTY 3 cut(s) 218, 326, 410
Asp700I GAANNNNTTC 2 cut(s) 100, 218
AsuII TTCGAA 1 cut(s) 406
BanII GRGCYC 1 cut(s) 792
BbsI GAAGAC 1 cut(s) 711
BbvI GCAGC 4 cut(s) 212, 427, 503, 679
BccI CCATC 1 cut(s) 225
BciT130I CCWGG 1 cut(s) 116
BciVI GTATCC 1 cut(s) 648
BfmI CTRYAG 1 cut(s) 441
BfuI GTATCC 1 cut(s) 648
BisI GCNGC 4 cut(s) 201, 441, 492, 693
BlsI GCNGC 4 cut(s) 202, 442, 493, 694
Bme1390I CCNGG 1 cut(s) 116
BmiI GGNNCC 2 cut(s) 236, 566
BmrFI CCNGG 1 cut(s) 116
BmrI ACTGGG 2 cut(s) 566, 763
BmsI GCATC 6 cut(s) 21, 142, 187, 415, 427, 534
BmuI ACTGGG 2 cut(s) 566, 763
BpiI GAAGAC 1 cut(s) 711
Bpu14I TTCGAA 1 cut(s) 406
BsaJI CCNNGG 1 cut(s) 518
Bsc4I CCNNNNNNNGG 1 cut(s) 535
Bse1I ACTGG 3 cut(s) 153, 572, 758
BseBI CCWGG 1 cut(s) 116
BseDI CCNNGG 1 cut(s) 518
BseGI GGATG 5 cut(s) 157, 236, 430, 525, 604
BseLI CCNNNNNNNGG 1 cut(s) 535
BseMII CTCAG 1 cut(s) 294
BseNI ACTGG 3 cut(s) 153, 572, 758
BseRI GAGGAG 1 cut(s) 483
BseXI GCAGC 4 cut(s) 212, 427, 503, 679
BslFI GGGAC 2 cut(s) 500, 683
BslI CCNNNNNNNGG 1 cut(s) 535
BsmFI GGGAC 2 cut(s) 500, 683
Bsp119I TTCGAA 1 cut(s) 406
Bsp1286I GDGCHC 1 cut(s) 792
Bsp143I GATC 3 cut(s) 241, 338, 601
Bsp19I CCATGG 1 cut(s) 518
BspCNI CTCAG 1 cut(s) 295
BspLI GGNNCC 2 cut(s) 236, 566
BspMAI CTGCAG 1 cut(s) 445
BspPI GGATC 3 cut(s) 236, 346, 596
BspT104I TTCGAA 1 cut(s) 406
BsrI ACTGG 3 cut(s) 153, 572, 758
BssECI CCNNGG 1 cut(s) 518
BssMI GATC 3 cut(s) 241, 338, 601
BssT1I CCWWGG 1 cut(s) 518
Bst2UI CCWGG 1 cut(s) 116
Bst4CI ACNGT 2 cut(s) 778, 875
Bst6I CTCTTC 1 cut(s) 644
BstAPI GCANNNNNTGC 1 cut(s) 152
BstBI TTCGAA 1 cut(s) 406
BstDEI CTNAG 2 cut(s) 59, 303
BstDSI CCRYGG 1 cut(s) 518
BstF5I GGATG 5 cut(s) 157, 236, 430, 525, 604
BstKTI GATC 3 cut(s) 244, 341, 604
BstMBI GATC 3 cut(s) 241, 338, 601
BstMWI GCNNNNNNNGC 5 cut(s) 107, 152, 209, 507, 898
BstNI CCWGG 1 cut(s) 116
BstNSI RCATGY 1 cut(s) 907
BstSCI CCNGG 1 cut(s) 114
BstSFI CTRYAG 1 cut(s) 441
BstV1I GCAGC 4 cut(s) 212, 427, 503, 679
BstV2I GAAGAC 1 cut(s) 711
BstX2I RGATCY 1 cut(s) 338
BstYI RGATCY 1 cut(s) 338
BsuI GTATCC 1 cut(s) 648
BtgI CCRYGG 1 cut(s) 518
BtgZI GCGATG 1 cut(s) 729
BtsCI GGATG 5 cut(s) 157, 236, 430, 525, 604
BtsIMutI CAGTG 3 cut(s) 146, 579, 774
Csp6I GTAC 1 cut(s) 559
CviAII CATG 4 cut(s) 164, 519, 766, 904
CviJI RGCY 9 cut(s) 68, 101, 283, 476, 494, 635, 692, 771, 790
CviKI_1 RGCY 9 cut(s) 68, 101, 283, 476, 494, 635, 692, 771, 790
CviQI GTAC 1 cut(s) 559
DdeI CTNAG 2 cut(s) 59, 303
DpnI GATC 3 cut(s) 243, 340, 603
DpnII GATC 3 cut(s) 241, 338, 601
DraI TTTAAA 1 cut(s) 639
Eam1104I CTCTTC 1 cut(s) 644
EarI CTCTTC 1 cut(s) 644
Eco130I CCWWGG 1 cut(s) 518
Eco24I GRGCYC 1 cut(s) 792
EcoRI GAATTC 1 cut(s) 218
EcoRII CCWGG 1 cut(s) 114
EcoT14I CCWWGG 1 cut(s) 518
EcoT22I ATGCAT 1 cut(s) 14
EcoT38I GRGCYC 1 cut(s) 792
ErhI CCWWGG 1 cut(s) 518
FaeI CATG 4 cut(s) 167, 522, 769, 907
FaqI GGGAC 2 cut(s) 500, 683
FatI CATG 4 cut(s) 163, 518, 765, 903
Fnu4HI GCNGC 4 cut(s) 201, 441, 492, 693
FokI GGATG 5 cut(s) 164, 243, 437, 512, 611
FriOI GRGCYC 1 cut(s) 792
Fsp4HI GCNGC 4 cut(s) 201, 441, 492, 693
GluI GCNGC 4 cut(s) 201, 441, 492, 693
Hin1II CATG 4 cut(s) 167, 522, 769, 907
HincII GTYRAC 1 cut(s) 871
HindII GTYRAC 1 cut(s) 871
HindIII AAGCTT 1 cut(s) 281
HinfI GANTC 4 cut(s) 36, 320, 403, 826
HpaI GTTAAC 1 cut(s) 871
Hpy166II GTNNAC 3 cut(s) 346, 852, 871
Hpy188I TCNGA 8 cut(s) 27, 95, 178, 387, 436, 530, 782, 882
Hpy8I GTNNAC 3 cut(s) 346, 852, 871
HpyAV CCTTC 2 cut(s) 74, 625
HpyCH4III ACNGT 2 cut(s) 778, 875
HpyCH4V TGCA 7 cut(s) 12, 146, 155, 200, 421, 443, 892
HpyF10VI GCNNNNNNNGC 5 cut(s) 107, 152, 209, 507, 898
HpyF3I CTNAG 2 cut(s) 59, 303
Hsp92II CATG 4 cut(s) 167, 522, 769, 907
KspAI GTTAAC 1 cut(s) 871
Kzo9I GATC 3 cut(s) 241, 338, 601
Lsp1109I GCAGC 4 cut(s) 212, 427, 503, 679
LweI GCATC 6 cut(s) 21, 142, 187, 415, 427, 534
MaeIII GTNAC 1 cut(s) 504
MalI GATC 3 cut(s) 243, 340, 603
MboI GATC 3 cut(s) 241, 338, 601
MboII GAAGA 6 cut(s) 96, 289, 329, 661, 716, 828
MflI RGATCY 1 cut(s) 338
MhlI GDGCHC 1 cut(s) 792
MluCI AATT 4 cut(s) 141, 218, 326, 410
MlyI GAGTC 1 cut(s) 30
MmeI TCCRAC 1 cut(s) 118
MnlI CCTC 6 cut(s) 180, 181, 276, 461, 645, 816
Mph1103I ATGCAT 1 cut(s) 14
MroXI GAANNNNTTC 2 cut(s) 100, 218
MseI TTAA 2 cut(s) 638, 870
MslI CAYNNNNRTG 2 cut(s) 229, 623
MspA1I CMGCKG 1 cut(s) 692
MspR9I CCNGG 1 cut(s) 116
MvaI CCWGG 1 cut(s) 116
MwoI GCNNNNNNNGC 5 cut(s) 107, 152, 209, 507, 898
NcoI CCATGG 1 cut(s) 518
NdeII GATC 3 cut(s) 241, 338, 601
NlaIII CATG 4 cut(s) 167, 522, 769, 907
NlaIV GGNNCC 2 cut(s) 236, 566
NsiI ATGCAT 1 cut(s) 14
NspI RCATGY 1 cut(s) 907
NspV TTCGAA 1 cut(s) 406
PciI ACATGT 1 cut(s) 903
PdmI GAANNNNTTC 2 cut(s) 100, 218
PfeI GAWTC 3 cut(s) 320, 403, 826
PkrI GCNGC 4 cut(s) 202, 442, 493, 694
PleI GAGTC 1 cut(s) 30
PpsI GAGTC 1 cut(s) 30
PscI ACATGT 1 cut(s) 903
Psp6I CCWGG 1 cut(s) 114
PspGI CCWGG 1 cut(s) 114
PspN4I GGNNCC 2 cut(s) 236, 566
PsrI GAACNNNNNNTAC 2 cut(s) 755, 787
PstI CTGCAG 1 cut(s) 445
PsuI RGATCY 1 cut(s) 338
PvuII CAGCTG 1 cut(s) 692
RsaI GTAC 1 cut(s) 560
RsaNI GTAC 1 cut(s) 559
RseI CAYNNNNRTG 2 cut(s) 229, 623
SaqAI TTAA 2 cut(s) 638, 870
SatI GCNGC 4 cut(s) 201, 441, 492, 693
Sau3AI GATC 3 cut(s) 241, 338, 601
SchI GAGTC 1 cut(s) 30
ScrFI CCNGG 1 cut(s) 116
SduI GDGCHC 1 cut(s) 792
SfaNI GCATC 6 cut(s) 21, 142, 187, 415, 427, 534
SfcI CTRYAG 1 cut(s) 441
SfuI TTCGAA 1 cut(s) 406
SmiMI CAYNNNNRTG 2 cut(s) 229, 623
Sse9I AATT 4 cut(s) 141, 218, 326, 410
StyD4I CCNGG 1 cut(s) 114
StyI CCWWGG 1 cut(s) 518
TaaI ACNGT 2 cut(s) 778, 875
TaqI TCGA 2 cut(s) 406, 801
TasI AATT 4 cut(s) 141, 218, 326, 410
TfiI GAWTC 3 cut(s) 320, 403, 826
Tru1I TTAA 2 cut(s) 638, 870
Tru9I TTAA 2 cut(s) 638, 870
TscAI CASTG 3 cut(s) 153, 579, 781
TseI GCWGC 4 cut(s) 200, 440, 491, 692
TspDTI ATGAA 4 cut(s) 180, 231, 290, 607
TspGWI ACGGA 1 cut(s) 415
TspRI CASTG 3 cut(s) 153, 579, 781
XapI RAATTY 3 cut(s) 218, 326, 410
XceI RCATGY 1 cut(s) 907
XmnI GAANNNNTTC 2 cut(s) 100, 218
Zsp2I ATGCAT 1 cut(s) 14
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.