Rroxscaffold_1G00044000

Belongs to the glycosyl hydrolase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0008270
Physical Location & Seq
Forward (+)
62718622 .. 62720887
2266 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00044000.1

Sequence Viewer

Length: 1269 bp
ATGAAGGATATCGGAGTAAATGCATACAGATTCTCAATATCGTGGCCAAGAATTTTTCCAAATGGAACAGGAGCACCCAACTCAGAAGGAATAAGCTACTACAACAGCCTTATAGATTCTTTGCTTGCAAAAGGGATCCAGCCTTTTGTAACTCTATATCATTGGGATCTTCCGCAGATGCTTGAAGACCGATATGAAGGATGGTTGAGCACACAGATAGTAAAGGACTTTGAACATTATGCCTTCACCTGCTTCCAAGCGTTTGGAGACAGAGTTAAGCACTGGATCACCATCAACGAGCCTCATGGTTATGCACTCCAAAGTTATGACTTGGGAATACAAGCTCCCGGGCGATGTTCTATTCTTGGTCACTTGTTTTGTAAGAAAGGAAATTCATCCGTTGAACCGTACATTGTTGCTCACAACATCCTCCTGTCTCATGCTGCAACTTATCATATGTACCAGAAATTTTTCAAGGAGAAACAAGTAGGTCAGGTTGGAATTGCACTGGATGCAAAATGGCATGAACCTCTATCGGAAAGTGATGAGGACATTGATGCAGCACATAGAGCGAATGAATTCACATTGGGATGGTTCCTTGATCCACTATTTTTTGGCGACTATCCTCTCTCGATGAAGAAGCTTGTGGGTAAGCGATTACCTGAGATTTCGCAGGAAGTATCAAAATTCTTGGTGGGATCTGTGGACTTTATTGGCATAAACCACTACACCACATTATATGCTCGGAATGACAGGACACGGATTCGAAAATTTATATTGCAGGATGCTACATCTGATGCTGCAGTCATTACAACACAATACAAAGGAGGAGTGGCGATTGGAGAAAAAGCAGCTTCTCGCTGGTTACGCATAGTCCCATGGGGCATCCGAAAGTTGGCAAGATACATCAAAGATAAGTACGGGAACCCCCCAGTGATGATAACAGAAAATGGTATGGATGATCCAAATAAACCTTTCATATCTATGGAAAAGGCTTTAAATGATGAGAAGAGGATACGATTTCACAGGGATTATCTCTCAAACCTATCAGCTGCTATAAGGGAAGACAAATGTGATGTTCGTGGTTATTTTGTGTGGTCATTACTTGACAACTGGGAATGGAACATGGGCTACACTGTCAGATTTGGGCTCTACTATGTCGATTACAAGAAGAACCTCACGAGGATTCCTAAATCTTCTGTTCAATGGTTTACAAGATTTCTCCAAGTTAACAATCATCAGAGTTTTGTGCAGACAAAGCACATGTAA

Protein Analysis

422

Amino Acids

48.9

Weight (kDa)

8.85

Isoelectric Point (pI)

30.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_1 PF00232 1 - 411 4e-127 Glycosyl hydrolase family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 257
Acc36I ACCTGC 1 cut(s) 257
AciI CCGC 1 cut(s) 173
AclWI GGATC 7 cut(s) 130, 143, 174, 293, 596, 706, 956
AcoI YGGCCR 1 cut(s) 44
AcsI RAATTY 6 cut(s) 51, 391, 467, 578, 686, 770
AfaI GTAC 3 cut(s) 410, 461, 920
AfiI CCNNNNNNNGG 1 cut(s) 895
AflIII ACRYGT 1 cut(s) 1263
AgsI TTSAA 5 cut(s) 185, 233, 404, 475, 1205
AluBI AGCT 5 cut(s) 96, 344, 643, 854, 1052
AluI AGCT 5 cut(s) 96, 344, 643, 854, 1052
Alw21I GWGCWC 2 cut(s) 76, 212
Alw26I GTCTC 2 cut(s) 261, 441
AlwI GGATC 7 cut(s) 130, 143, 174, 293, 596, 706, 956
Ama87I CYCGRG 1 cut(s) 347
AoxI GGCC 1 cut(s) 44
ApeKI GCWGC 5 cut(s) 443, 560, 800, 851, 1052
ApoI RAATTY 6 cut(s) 51, 391, 467, 578, 686, 770
Asp700I GAANNNNTTC 2 cut(s) 470, 578
AsuC2I CCSGG 2 cut(s) 348, 349
AsuHPI GGTGA 2 cut(s) 238, 280
AsuII TTCGAA 1 cut(s) 766
AvaI CYCGRG 1 cut(s) 347
BalI TGGCCA 1 cut(s) 46
BamHI GGATCC 1 cut(s) 135
BanII GRGCYC 1 cut(s) 1152
BauI CACGAG 1 cut(s) 1180
BbsI GAAGAC 2 cut(s) 192, 1071
Bbv12I GWGCWC 2 cut(s) 76, 212
BbvI GCAGC 5 cut(s) 430, 572, 787, 863, 1039
BccI CCATC 3 cut(s) 195, 299, 585
BciVI GTATCC 1 cut(s) 1008
BcnI CCSGG 2 cut(s) 348, 349
BcoDI GTCTC 2 cut(s) 261, 441
BfmI CTRYAG 1 cut(s) 801
BfuAI ACCTGC 1 cut(s) 257
BfuI GTATCC 1 cut(s) 1008
BisI GCNGC 5 cut(s) 444, 561, 801, 852, 1053
BlsI GCNGC 5 cut(s) 445, 562, 802, 853, 1054
Bme1390I CCNGG 2 cut(s) 348, 349
BmeT110I CYCGRG 1 cut(s) 347
BmiI GGNNCC 3 cut(s) 137, 596, 926
BmrFI CCNGG 2 cut(s) 348, 349
BmrI ACTGGG 2 cut(s) 926, 1123
BmsI GCATC 6 cut(s) 168, 502, 547, 775, 787, 894
BmuI ACTGGG 2 cut(s) 926, 1123
BpiI GAAGAC 2 cut(s) 192, 1071
Bpu14I TTCGAA 1 cut(s) 766
BpuMI CCSGG 2 cut(s) 348, 349
BsaBI GATNNNNATC 1 cut(s) 290
BsaJI CCNNGG 2 cut(s) 347, 878
BsaXI ACNNNNNCTCC 2 cut(s) 258, 288
Bsc4I CCNNNNNNNGG 1 cut(s) 895
Bse1I ACTGG 4 cut(s) 287, 513, 932, 1118
Bse8I GATNNNNATC 1 cut(s) 290
BseDI CCNNGG 2 cut(s) 347, 878
BseGI GGATG 8 cut(s) 206, 395, 426, 517, 596, 790, 885, 964
BseJI GATNNNNATC 1 cut(s) 290
BseLI CCNNNNNNNGG 1 cut(s) 895
BseMII CTCAG 2 cut(s) 96, 654
BseNI ACTGG 4 cut(s) 287, 513, 932, 1118
BseRI GAGGAG 1 cut(s) 843
BseXI GCAGC 5 cut(s) 430, 572, 787, 863, 1039
BshFI GGCC 1 cut(s) 46
BsiHKAI GWGCWC 2 cut(s) 76, 212
BsiHKCI CYCGRG 1 cut(s) 347
BsiSI CCGG 1 cut(s) 348
BslFI GGGAC 1 cut(s) 860
BslI CCNNNNNNNGG 1 cut(s) 895
BsmAI GTCTC 2 cut(s) 261, 441
BsmFI GGGAC 1 cut(s) 860
BsnI GGCC 1 cut(s) 46
BsoBI CYCGRG 1 cut(s) 347
Bsp119I TTCGAA 1 cut(s) 766
Bsp1286I GDGCHC 3 cut(s) 76, 212, 1152
Bsp143I GATC 6 cut(s) 135, 166, 285, 601, 698, 961
Bsp19I CCATGG 1 cut(s) 878
BspACI CCGC 1 cut(s) 173
BspANI GGCC 1 cut(s) 46
BspCNI CTCAG 2 cut(s) 95, 655
BspLI GGNNCC 3 cut(s) 137, 596, 926
BspMAI CTGCAG 1 cut(s) 805
BspMI ACCTGC 1 cut(s) 257
BspPI GGATC 7 cut(s) 130, 143, 174, 293, 596, 706, 956
BspT104I TTCGAA 1 cut(s) 766
BsrI ACTGG 4 cut(s) 287, 513, 932, 1118
BssECI CCNNGG 2 cut(s) 347, 878
BssMI GATC 6 cut(s) 135, 166, 285, 601, 698, 961
BssSI CACGAG 1 cut(s) 1180
BssT1I CCWWGG 1 cut(s) 878
Bst2BI CACGAG 1 cut(s) 1180
Bst4CI ACNGT 2 cut(s) 408, 1138
Bst6I CTCTTC 1 cut(s) 1004
BstAPI GCANNNNNTGC 1 cut(s) 512
BstBI TTCGAA 1 cut(s) 766
BstC8I GCNNGC 1 cut(s) 126
BstDEI CTNAG 2 cut(s) 82, 663
BstDSI CCRYGG 1 cut(s) 878
BstF5I GGATG 8 cut(s) 206, 395, 426, 517, 596, 790, 885, 964
BstKTI GATC 6 cut(s) 138, 169, 288, 604, 701, 964
BstMAI GTCTC 2 cut(s) 261, 441
BstMBI GATC 6 cut(s) 135, 166, 285, 601, 698, 961
BstMWI GCNNNNNNNGC 4 cut(s) 512, 569, 867, 1258
BstNSI RCATGY 1 cut(s) 1267
BstSCI CCNGG 2 cut(s) 346, 347
BstSFI CTRYAG 1 cut(s) 801
BstV1I GCAGC 5 cut(s) 430, 572, 787, 863, 1039
BstV2I GAAGAC 2 cut(s) 192, 1071
BstX2I RGATCY 3 cut(s) 135, 166, 698
BstXI CCANNNNNNTGG 1 cut(s) 263
BstYI RGATCY 3 cut(s) 135, 166, 698
BsuI GTATCC 1 cut(s) 1008
BsuRI GGCC 1 cut(s) 46
BtgI CCRYGG 1 cut(s) 878
BtgZI GCGATG 1 cut(s) 367
BtsCI GGATG 8 cut(s) 206, 395, 426, 517, 596, 790, 885, 964
BtsIMutI CAGTG 4 cut(s) 280, 506, 939, 1134
BveI ACCTGC 1 cut(s) 257
Cac8I GCNNGC 1 cut(s) 126
Cfr9I CCCGGG 1 cut(s) 347
Csp6I GTAC 3 cut(s) 409, 460, 919
CviAII CATG 6 cut(s) 305, 440, 524, 879, 1126, 1264
CviQI GTAC 3 cut(s) 409, 460, 919
DdeI CTNAG 2 cut(s) 82, 663
DpnI GATC 6 cut(s) 137, 168, 287, 603, 700, 963
DpnII GATC 6 cut(s) 135, 166, 285, 601, 698, 961
DraI TTTAAA 1 cut(s) 999
EaeI YGGCCR 1 cut(s) 44
Eam1104I CTCTTC 1 cut(s) 1004
EarI CTCTTC 1 cut(s) 1004
Eco130I CCWWGG 1 cut(s) 878
Eco24I GRGCYC 1 cut(s) 1152
Eco32I GATATC 1 cut(s) 10
Eco88I CYCGRG 1 cut(s) 347
EcoRI GAATTC 1 cut(s) 578
EcoRV GATATC 1 cut(s) 10
EcoT14I CCWWGG 1 cut(s) 878
EcoT22I ATGCAT 1 cut(s) 25
EcoT38I GRGCYC 1 cut(s) 1152
ErhI CCWWGG 1 cut(s) 878
FaeI CATG 6 cut(s) 308, 443, 527, 882, 1129, 1267
FaqI GGGAC 1 cut(s) 860
FatI CATG 6 cut(s) 304, 439, 523, 878, 1125, 1263
FauNDI CATATG 1 cut(s) 456
Fnu4HI GCNGC 5 cut(s) 444, 561, 801, 852, 1053
FokI GGATG 8 cut(s) 213, 382, 413, 524, 603, 797, 872, 971
FriOI GRGCYC 1 cut(s) 1152
Fsp4HI GCNGC 5 cut(s) 444, 561, 801, 852, 1053
GluI GCNGC 5 cut(s) 444, 561, 801, 852, 1053
HaeIII GGCC 1 cut(s) 46
HapII CCGG 1 cut(s) 348
Hin1II CATG 6 cut(s) 308, 443, 527, 882, 1129, 1267
HincII GTYRAC 1 cut(s) 1231
HindII GTYRAC 1 cut(s) 1231
HindIII AAGCTT 1 cut(s) 641
HinfI GANTC 4 cut(s) 30, 116, 763, 1186
HpaI GTTAAC 1 cut(s) 1231
HpaII CCGG 1 cut(s) 348
HphI GGTGA 2 cut(s) 238, 280
Hpy166II GTNNAC 3 cut(s) 706, 1212, 1231
Hpy188I TCNGA 8 cut(s) 14, 85, 538, 747, 796, 890, 1142, 1242
Hpy188III TCNNGA 2 cut(s) 631, 1180
Hpy8I GTNNAC 3 cut(s) 706, 1212, 1231
HpyAV CCTTC 3 cut(s) 80, 191, 253
HpyCH4III ACNGT 2 cut(s) 408, 1138
HpyF10VI GCNNNNNNNGC 4 cut(s) 512, 569, 867, 1258
HpyF3I CTNAG 2 cut(s) 82, 663
Hsp92II CATG 6 cut(s) 308, 443, 527, 882, 1129, 1267
KspAI GTTAAC 1 cut(s) 1231
Kzo9I GATC 6 cut(s) 135, 166, 285, 601, 698, 961
LmnI GCTCC 2 cut(s) 71, 349
Lsp1109I GCAGC 5 cut(s) 430, 572, 787, 863, 1039
LweI GCATC 6 cut(s) 168, 502, 547, 775, 787, 894
MaeIII GTNAC 3 cut(s) 148, 368, 864
MalI GATC 6 cut(s) 137, 168, 287, 603, 700, 963
MboI GATC 6 cut(s) 135, 166, 285, 601, 698, 961
MboII GAAGA 7 cut(s) 161, 197, 649, 1021, 1076, 1183, 1188
MflI RGATCY 3 cut(s) 135, 166, 698
MhlI GDGCHC 3 cut(s) 76, 212, 1152
MlsI TGGCCA 1 cut(s) 46
MluCI AATT 7 cut(s) 51, 391, 467, 501, 578, 686, 770
MluNI TGGCCA 1 cut(s) 46
MmeI TCCRAC 1 cut(s) 478
MnlI CCTC 9 cut(s) 312, 440, 540, 541, 636, 821, 1005, 1176, 1187
Mox20I TGGCCA 1 cut(s) 46
Mph1103I ATGCAT 1 cut(s) 25
MroXI GAANNNNTTC 2 cut(s) 470, 578
MscI TGGCCA 1 cut(s) 46
MseI TTAA 3 cut(s) 276, 998, 1230
MslI CAYNNNNRTG 3 cut(s) 309, 589, 983
Msp20I TGGCCA 1 cut(s) 46
MspA1I CMGCKG 1 cut(s) 1052
MspI CCGG 1 cut(s) 348
MspR9I CCNGG 2 cut(s) 348, 349
MwoI GCNNNNNNNGC 4 cut(s) 512, 569, 867, 1258
NciI CCSGG 2 cut(s) 348, 349
NcoI CCATGG 1 cut(s) 878
NdeI CATATG 1 cut(s) 456
NdeII GATC 6 cut(s) 135, 166, 285, 601, 698, 961
NlaIII CATG 6 cut(s) 308, 443, 527, 882, 1129, 1267
NlaIV GGNNCC 3 cut(s) 137, 596, 926
NmuCI GTSAC 1 cut(s) 368
NsiI ATGCAT 1 cut(s) 25
NspI RCATGY 1 cut(s) 1267
NspV TTCGAA 1 cut(s) 766
PaqCI CACCTGC 1 cut(s) 257
PciI ACATGT 1 cut(s) 1263
PdmI GAANNNNTTC 2 cut(s) 470, 578
PfeI GAWTC 4 cut(s) 30, 116, 763, 1186
PkrI GCNGC 5 cut(s) 445, 562, 802, 853, 1054
PscI ACATGT 1 cut(s) 1263
PspN4I GGNNCC 3 cut(s) 137, 596, 926
PsrI GAACNNNNNNTAC 2 cut(s) 1115, 1147
PstI CTGCAG 1 cut(s) 805
PsuI RGATCY 3 cut(s) 135, 166, 698
PvuII CAGCTG 1 cut(s) 1052
RsaI GTAC 3 cut(s) 410, 461, 920
RsaNI GTAC 3 cut(s) 409, 460, 919
RseI CAYNNNNRTG 3 cut(s) 309, 589, 983
SaqAI TTAA 3 cut(s) 276, 998, 1230
SatI GCNGC 5 cut(s) 444, 561, 801, 852, 1053
Sau3AI GATC 6 cut(s) 135, 166, 285, 601, 698, 961
ScrFI CCNGG 2 cut(s) 348, 349
SduI GDGCHC 3 cut(s) 76, 212, 1152
SfaNI GCATC 6 cut(s) 168, 502, 547, 775, 787, 894
SfcI CTRYAG 1 cut(s) 801
SfuI TTCGAA 1 cut(s) 766
SmaI CCCGGG 1 cut(s) 349
SmiMI CAYNNNNRTG 3 cut(s) 309, 589, 983
Sse9I AATT 7 cut(s) 51, 391, 467, 501, 578, 686, 770
SsiI CCGC 1 cut(s) 173
StyD4I CCNGG 2 cut(s) 346, 347
StyI CCWWGG 1 cut(s) 878
TaaI ACNGT 2 cut(s) 408, 1138
TaqI TCGA 3 cut(s) 632, 766, 1161
TaqII GACCGA 1 cut(s) 204
TasI AATT 7 cut(s) 51, 391, 467, 501, 578, 686, 770
TfiI GAWTC 4 cut(s) 30, 116, 763, 1186
Tru1I TTAA 3 cut(s) 276, 998, 1230
Tru9I TTAA 3 cut(s) 276, 998, 1230
TscAI CASTG 4 cut(s) 287, 513, 939, 1141
TseFI GTSAC 1 cut(s) 368
TseI GCWGC 5 cut(s) 443, 560, 800, 851, 1052
Tsp45I GTSAC 1 cut(s) 368
TspDTI ATGAA 7 cut(s) 17, 210, 384, 540, 591, 650, 967
TspGWI ACGGA 2 cut(s) 388, 775
TspMI CCCGGG 1 cut(s) 347
TspRI CASTG 4 cut(s) 287, 513, 939, 1141
XapI RAATTY 6 cut(s) 51, 391, 467, 578, 686, 770
XceI RCATGY 1 cut(s) 1267
XmaI CCCGGG 1 cut(s) 347
XmnI GAANNNNTTC 2 cut(s) 470, 578
Zsp2I ATGCAT 1 cut(s) 25
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.