Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0015573

Physical Location & Seq

Genomic Coordinates Reverse (-)

30437181 .. 30442321

Gene Structure 5141 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1220100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1359 bp

ATGTGGGCCTTATCCTGCGGCACGTCTGCTTATCCTACGCTCGCTCCCAGAGCTTCTGTACCCAGGGCTACTACTCGCTCAAGCTCTCCACTCTCAGTCCCTACCAATCACCATTTTCATCCGAAAGACTCTCCTTTTGTTCCAGAGGTTTCGGACGCAGTGGACTCGTTGTATTCAGAGTTCAGAGCCGTGGATAATTTGGTTGCACGCAATACCACCCGTGTTCTTAAAGCTTTCCAGAATGCTCGAGTTGGATCACATCACTTTGCTGGATGCACTGGTTATGGTCATGATGAAGCTGGGGGACGTGAAGCGCTGGACCAAGCTTTTGCTGAAATTGTTGGGGCTGAATCTGCTATAGTTCGCTCACAGTTTTTTTCAGGAACTCATGCTATAACATGTGCTTTATTTGCTTTTCTAAGGCCAGGGGATGAGCTTTTGGCAGTTGCTGGTCCTCCCTATGACACCTTGGAGGAAGTTATTGGAAAGAGAGACTCTCATGGGATGGGGTCCCTGACAGATTTTGGAGTGAAATACCGAGAAGTTCCACTTGCTGAAGATGGCGGCCTTAACTGGGATGTACTTATCCATGCTTTGAGACCTGAAACAAAATGTGCGCTCATACAGAGGTCATGTGGTTATTCATGGCGTCGGAGTTTAAGTGTAGATGAGATAGGGCGAGCAATTAAGATAATTAAGACGCAGAATCCTAACTGCTTGGTCATGGTGGATAACTGTTATGGTGAATTTGTGGAAAGCATTGAACCTCCAATGGTGGGTGCAGATTTGATTGCGGGCAGTTTGATAAAAAATCCTGGTGGGACTATAGCACCATGTGGTGGATATGTTGCAGGGAGAGAAAAATGGGTAAAAGCAGCATCAGCTCGTCTGTCTGCACCTGGCCTTGGAGTTGATTGTGGCGCAACTCCTGGTGACATTATGCGATCATTTTTCCAGGGTTTGTTCCTTTCACCTCAAATGGTAGGCGAGGCTATCAAGGGCACTTTAGTGGTAGCTGAAGTTATGGCAGCCAGAGGGTATAAGGTGCAGCCACTTCCTCGTATTCCTCGTCATGACACAGTCCAGGCCGTACAGCTTGGAAGCCGTGAGCGCCTTCTTGCTTTCTGCGAGGCTGTACAGAGAAACTCCCCAGTGGGTTCATTTACCAAACCAGTGGCTGGTGCAACTCCTGGATATGCATCAGAGGTGATCTTTGCTGATGGAACCTTCATCGATGGGAGTACAAGTGAGCTTTCATGCGATGGACCACTGAGAGAGCCATTTGCTGTGTTTTGCCAGGGTGGCTCCCATTGGACTCAGTGGGGACTAGTTCTTGGAGAGGTTTTAAAAGCCATATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003962 GO:0004123 GO:0016740 GO:0016765 GO:0016829 GO:0016846

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Met_gamma_lyase

Protein Analysis

453

Amino Acids

48.31

Weight (kDa)

6.14

Isoelectric Point (pI)

32.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Met_gamma_lyase	PF06838	52 - 450	2.7e-164	Methionine gamma-lyase
Cys_Met_Meta_PP	PF01053	70 - 294	1e-06	Cys/Met metabolism PLP-dependent enzyme

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015573)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g20850 FvH4_3g20850 FvH4_3g20850
malus_domestica	MD03G1220100.v1.1
prunus_persica	Prupe.4G188800_v2.0.a1 Prupe.4G188800_v2.0.a1
pyrus_communis	pycom03g16940
rosa_chinensis	RchiOBHm_Chr5g0035161
rosa_laevigata	RLG00000033603
rosa_multiflora	Rmu_sc0000879.1_g000001 Rmu_sc0008176.1_g000007
rosa_roxburghii	Rroxscaffold_1G00045250
rosa_rugosa	Rorug05G0150400
rosa_samantha	Rh5BG242800 Rh5DG251600
rosa_wichuraiana	Rw5G022220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	2 cut(s)	839, 1178
AciI	CCGC	3 cut(s)	18, 564, 794
AclWI	GGATC	1 cut(s)	262
AcsI	RAATTY	1 cut(s)	746
AcuI	CTGAAG	2 cut(s)	576, 1038
AcyI	GRCGYC	1 cut(s)	649
AdeI	CACNNNGTG	1 cut(s)	836
AfaI	GTAC	5 cut(s)	60, 582, 1092, 1137, 1243
AfeI	AGCGCT	1 cut(s)	315
AfiI	CCNNNNNNNGG	5 cut(s)	574, 776, 839, 905, 1178
AflIII	ACRYGT	1 cut(s)	398
AgsI	TTSAA	1 cut(s)	764
AhlI	ACTAGT	1 cut(s)	1327
AjiI	CACGTC	2 cut(s)	24, 308
AjnI	CCWGG	9 cut(s)	62, 424, 814, 898, 928, 954, 1083, 1189, 1296
AleI	CACNNNNGTG	1 cut(s)	1007
Alw26I	GTCTC	2 cut(s)	486, 592
AlwI	GGATC	1 cut(s)	262
AlwNI	CAGNNNCTG	2 cut(s)	449, 1178
Ama87I	CYCGRG	1 cut(s)	246
Aor51HI	AGCGCT	1 cut(s)	315
AoxI	GGCC	5 cut(s)	6, 422, 565, 901, 1086
ApeKI	GCWGC	3 cut(s)	875, 1028, 1048
ApoI	RAATTY	1 cut(s)	746
ArsI	GACNNNNNNTTYG	4 cut(s)	119, 151, 311, 343
AspLEI	GCGC	4 cut(s)	316, 619, 923, 1113
AspS9I	GGNCC	5 cut(s)	6, 319, 452, 510, 1265
AsuHPI	GGTGA	5 cut(s)	101, 755, 944, 963, 1219
AvaI	CYCGRG	1 cut(s)	246
AvaII	GGWCC	4 cut(s)	319, 452, 510, 1265
BaeGI	GKGCMC	1 cut(s)	1004
BbvI	GCAGC	3 cut(s)	887, 1040, 1060
BccI	CCATC	5 cut(s)	499, 554, 1214, 1229, 1256
BceAI	ACGGC	3 cut(s)	173, 1073, 1089
BcgI	CGANNNNNNTGC	2 cut(s)	147, 181
BciT130I	CCWGG	9 cut(s)	64, 426, 816, 900, 930, 956, 1085, 1191, 1298
BcoDI	GTCTC	2 cut(s)	486, 592
BcuI	ACTAGT	1 cut(s)	1327
BfaI	CTAG	1 cut(s)	1328
BfmI	CTRYAG	2 cut(s)	357, 825
BfoI	RGCGCY	2 cut(s)	317, 1114
BglI	GCCNNNNNGGC	1 cut(s)	1302
BisI	GCNGC	5 cut(s)	19, 565, 876, 1029, 1049
BlsI	GCNGC	5 cut(s)	20, 566, 877, 1030, 1050
Bme1390I	CCNGG	9 cut(s)	64, 426, 816, 900, 930, 956, 1085, 1191, 1298
Bme18I	GGWCC	4 cut(s)	319, 452, 510, 1265
BmeT110I	CYCGRG	1 cut(s)	246
BmgBI	CACGTC	2 cut(s)	24, 308
BmgT120I	GGNCC	5 cut(s)	6, 319, 452, 510, 1265
BmiI	GGNNCC	4 cut(s)	511, 512, 1225, 1306
BmrFI	CCNGG	9 cut(s)	64, 426, 816, 900, 930, 956, 1085, 1191, 1298
BmrI	ACTGGG	2 cut(s)	583, 1145
BmsI	GCATC	3 cut(s)	263, 887, 1208
BmuI	ACTGGG	2 cut(s)	583, 1145
BplI	GAGNNNNNCTC	2 cut(s)	481, 513
BpuEI	CTTGAG	1 cut(s)	64
Bsa29I	ATCGAT	1 cut(s)	1233
BsaBI	GATNNNNATC	1 cut(s)	1198
BsaHI	GRCGYC	1 cut(s)	649
BsaI	GGTCTC	1 cut(s)	592
BsaJI	CCNNGG	8 cut(s)	62, 63, 189, 425, 468, 904, 955, 1297
BsaXI	ACNNNNNCTCC	6 cut(s)	28, 58, 646, 676, 1231, 1261
Bsc4I	CCNNNNNNNGG	5 cut(s)	574, 776, 839, 905, 1178
Bse1I	ACTGG	4 cut(s)	283, 578, 1151, 1172
Bse8I	GATNNNNATC	1 cut(s)	1198
BseBI	CCWGG	9 cut(s)	64, 426, 816, 900, 930, 956, 1085, 1191, 1298
BseCI	ATCGAT	1 cut(s)	1233
BseDI	CCNNGG	8 cut(s)	62, 63, 189, 425, 468, 904, 955, 1297
BseGI	GGATG	5 cut(s)	118, 278, 436, 510, 583
BseJI	GATNNNNATC	1 cut(s)	1198
BseLI	CCNNNNNNNGG	5 cut(s)	574, 776, 839, 905, 1178
BseMII	CTCAG	3 cut(s)	108, 1262, 1331
BseNI	ACTGG	4 cut(s)	283, 578, 1151, 1172
BseSI	GKGCMC	1 cut(s)	1004
BseXI	GCAGC	3 cut(s)	887, 1040, 1060
BseYI	CCCAGC	1 cut(s)	299
BsgI	GTGCAG	3 cut(s)	801, 879, 1067
BshFI	GGCC	5 cut(s)	8, 424, 567, 903, 1088
BshVI	ATCGAT	1 cut(s)	1233
BsiHKCI	CYCGRG	1 cut(s)	246
BslFI	GGGAC	5 cut(s)	83, 318, 496, 835, 1338
BslI	CCNNNNNNNGG	5 cut(s)	574, 776, 839, 905, 1178
BsmAI	GTCTC	2 cut(s)	486, 592
BsmFI	GGGAC	5 cut(s)	83, 318, 496, 835, 1338
BsmI	GAATGC	1 cut(s)	247
BsnI	GGCC	5 cut(s)	8, 424, 567, 903, 1088
Bso31I	GGTCTC	1 cut(s)	592
BsoBI	CYCGRG	1 cut(s)	246
Bsp1286I	GDGCHC	1 cut(s)	1004
Bsp1407I	TGTACA	1 cut(s)	1135
Bsp143I	GATC	3 cut(s)	254, 944, 1209
BspACI	CCGC	3 cut(s)	18, 564, 794
BspANI	GGCC	5 cut(s)	8, 424, 567, 903, 1088
BspCNI	CTCAG	3 cut(s)	107, 1263, 1330
BspDI	ATCGAT	1 cut(s)	1233
BspHI	TCATGA	2 cut(s)	289, 1072
BspLI	GGNNCC	4 cut(s)	511, 512, 1225, 1306
BspPI	GGATC	1 cut(s)	262
BspTNI	GGTCTC	1 cut(s)	592
BsrGI	TGTACA	1 cut(s)	1135
BsrI	ACTGG	4 cut(s)	283, 578, 1151, 1172
BssECI	CCNNGG	8 cut(s)	62, 63, 189, 425, 468, 904, 955, 1297
BssMI	GATC	3 cut(s)	254, 944, 1209
BssNI	GRCGYC	1 cut(s)	649
BssT1I	CCWWGG	2 cut(s)	468, 904
Bst2UI	CCWGG	9 cut(s)	64, 426, 816, 900, 930, 956, 1085, 1191, 1298
Bst4CI	ACNGT	3 cut(s)	372, 737, 1081
BstACI	GRCGYC	1 cut(s)	649
BstAUI	TGTACA	1 cut(s)	1135
BstC8I	GCNNGC	4 cut(s)	42, 208, 681, 796
BstDEI	CTNAG	4 cut(s)	94, 419, 1271, 1317
BstDSI	CCRYGG	1 cut(s)	189
BstF5I	GGATG	5 cut(s)	118, 278, 436, 510, 583
BstH2I	RGCGCY	2 cut(s)	317, 1114
BstHHI	GCGC	4 cut(s)	316, 619, 923, 1113
BstKTI	GATC	3 cut(s)	257, 947, 1212
BstMAI	GTCTC	2 cut(s)	486, 592
BstMBI	GATC	3 cut(s)	254, 944, 1209
BstMWI	GCNNNNNNNGC	6 cut(s)	50, 353, 410, 881, 1110, 1302
BstNI	CCWGG	9 cut(s)	64, 426, 816, 900, 930, 956, 1085, 1191, 1298
BstNSI	RCATGY	1 cut(s)	402
BstSCI	CCNGG	9 cut(s)	62, 424, 814, 898, 928, 954, 1083, 1189, 1296
BstSFI	CTRYAG	2 cut(s)	357, 825
BstSLI	GKGCMC	1 cut(s)	1004
BstV1I	GCAGC	3 cut(s)	887, 1040, 1060
BstXI	CCANNNNNNTGG	1 cut(s)	1174
Bsu15I	ATCGAT	1 cut(s)	1233
BsuRI	GGCC	5 cut(s)	8, 424, 567, 903, 1088
BsuTUI	ATCGAT	1 cut(s)	1233
BtgI	CCRYGG	1 cut(s)	189
BtgZI	GCGATG	1 cut(s)	1275
BtrI	CACGTC	2 cut(s)	24, 308
BtsCI	GGATG	5 cut(s)	118, 278, 436, 510, 583
BtsI	GCAGTG	1 cut(s)	165
BtsIMutI	CAGTG	6 cut(s)	165, 276, 1158, 1179, 1268, 1325
Cac8I	GCNNGC	4 cut(s)	42, 208, 681, 796
CaiI	CAGNNNCTG	2 cut(s)	449, 1178
CciI	TCATGA	2 cut(s)	289, 1072
CfoI	GCGC	4 cut(s)	316, 619, 923, 1113
Cfr13I	GGNCC	5 cut(s)	6, 319, 452, 510, 1265
ClaI	ATCGAT	1 cut(s)	1233
CseI	GACGC	3 cut(s)	164, 638, 709
Csp6I	GTAC	5 cut(s)	59, 581, 1091, 1136, 1242
CviQI	GTAC	5 cut(s)	59, 581, 1091, 1136, 1242
DdeI	CTNAG	4 cut(s)	94, 419, 1271, 1317
DpnI	GATC	3 cut(s)	256, 946, 1211
DpnII	GATC	3 cut(s)	254, 944, 1209
DraI	TTTAAA	1 cut(s)	1347
DraIII	CACNNNGTG	1 cut(s)	836
Eco130I	CCWWGG	2 cut(s)	468, 904
Eco31I	GGTCTC	1 cut(s)	592
Eco47I	GGWCC	4 cut(s)	319, 452, 510, 1265
Eco47III	AGCGCT	1 cut(s)	315
Eco57I	CTGAAG	2 cut(s)	576, 1038
Eco88I	CYCGRG	1 cut(s)	246
EcoO109I	RGGNCCY	1 cut(s)	510
EcoRII	CCWGG	9 cut(s)	62, 424, 814, 898, 928, 954, 1083, 1189, 1296
EcoT14I	CCWWGG	2 cut(s)	468, 904
EcoT22I	ATGCAT	1 cut(s)	1201
ErhI	CCWWGG	2 cut(s)	468, 904
FalI	AAGNNNNNCTT	2 cut(s)	534, 566
FaqI	GGGAC	5 cut(s)	83, 318, 496, 835, 1338
FauI	CCCGC	1 cut(s)	787
Fnu4HI	GCNGC	5 cut(s)	19, 565, 876, 1029, 1049
FokI	GGATG	5 cut(s)	105, 285, 443, 517, 590
Fsp4HI	GCNGC	5 cut(s)	19, 565, 876, 1029, 1049
FspBI	CTAG	1 cut(s)	1328
GlaI	GCGC	4 cut(s)	315, 618, 922, 1112
GluI	GCNGC	5 cut(s)	19, 565, 876, 1029, 1049
GsaI	CCCAGC	1 cut(s)	303
HaeII	RGCGCY	2 cut(s)	317, 1114
HaeIII	GGCC	5 cut(s)	8, 424, 567, 903, 1088
HgaI	GACGC	3 cut(s)	164, 638, 709
HhaI	GCGC	4 cut(s)	316, 619, 923, 1113
Hin1I	GRCGYC	1 cut(s)	649
Hin6I	GCGC	4 cut(s)	314, 617, 921, 1111
HinP1I	GCGC	4 cut(s)	314, 617, 921, 1111
HindIII	AAGCTT	2 cut(s)	231, 324
HinfI	GANTC	6 cut(s)	128, 164, 350, 494, 706, 1315
HphI	GGTGA	5 cut(s)	101, 755, 944, 963, 1219
Hpy166II	GTNNAC	1 cut(s)	163
Hpy188I	TCNGA	6 cut(s)	123, 154, 178, 185, 654, 1204
Hpy188III	TCNNGA	5 cut(s)	143, 238, 290, 381, 1073
Hpy8I	GTNNAC	1 cut(s)	163
Hpy99I	CGWCG	1 cut(s)	654
HpyAV	CCTTC	2 cut(s)	1124, 1237
HpyCH4III	ACNGT	3 cut(s)	372, 737, 1081
HpyCH4IV	ACGT	2 cut(s)	23, 307
HpyCH4V	TGCA	8 cut(s)	206, 276, 782, 851, 896, 1048, 1184, 1199
HpyF10VI	GCNNNNNNNGC	6 cut(s)	50, 353, 410, 881, 1110, 1302
HpyF3I	CTNAG	4 cut(s)	94, 419, 1271, 1317
HpySE526I	ACGT	2 cut(s)	23, 307
Hsp92I	GRCGYC	1 cut(s)	649
HspAI	GCGC	4 cut(s)	314, 617, 921, 1111
KflI	GGGWCCC	1 cut(s)	510
Kzo9I	GATC	3 cut(s)	254, 944, 1209
LmnI	GCTCC	2 cut(s)	49, 1310
Lsp1109I	GCAGC	3 cut(s)	887, 1040, 1060
LweI	GCATC	3 cut(s)	263, 887, 1208
MaeI	CTAG	1 cut(s)	1328
MaeII	ACGT	2 cut(s)	23, 307
MaeIII	GTNAC	1 cut(s)	932
MalI	GATC	3 cut(s)	256, 946, 1211
MboI	GATC	3 cut(s)	254, 944, 1209
MboII	GAAGA	1 cut(s)	569
MhlI	GDGCHC	1 cut(s)	1004
MluCI	AATT	5 cut(s)	196, 336, 684, 693, 746
MlyI	GAGTC	4 cut(s)	122, 158, 488, 1309
MmeI	TCCRAC	2 cut(s)	232, 632
Mph1103I	ATGCAT	1 cut(s)	1201
MseI	TTAA	6 cut(s)	228, 570, 659, 687, 696, 1346
MslI	CAYNNNNRTG	1 cut(s)	1007
MspR9I	CCNGG	9 cut(s)	64, 426, 816, 900, 930, 956, 1085, 1191, 1298
Mva1269I	GAATGC	1 cut(s)	247
MvaI	CCWGG	9 cut(s)	64, 426, 816, 900, 930, 956, 1085, 1191, 1298
MwoI	GCNNNNNNNGC	6 cut(s)	50, 353, 410, 881, 1110, 1302
NdeII	GATC	3 cut(s)	254, 944, 1209
NlaIV	GGNNCC	4 cut(s)	511, 512, 1225, 1306
NmuCI	GTSAC	1 cut(s)	932
NsiI	ATGCAT	1 cut(s)	1201
NspI	RCATGY	1 cut(s)	402
OliI	CACNNNNGTG	1 cut(s)	1007
PaeR7I	CTCGAG	1 cut(s)	246
PagI	TCATGA	2 cut(s)	289, 1072
PasI	CCCWGGG	1 cut(s)	63
PciI	ACATGT	1 cut(s)	398
PcsI	WCGNNNNNNNCGW	1 cut(s)	1066
PctI	GAATGC	1 cut(s)	247
PfeI	GAWTC	2 cut(s)	350, 706
PflFI	GACNNNGTC	1 cut(s)	1079
PflMI	CCANNNNNTGG	2 cut(s)	839, 1178
PfoI	TCCNGGA	1 cut(s)	1189
PkrI	GCNGC	5 cut(s)	20, 566, 877, 1030, 1050
PleI	GAGTC	4 cut(s)	122, 158, 488, 1309
PpsI	GAGTC	4 cut(s)	122, 158, 488, 1309
PpuMI	RGGWCCY	1 cut(s)	510
PscI	ACATGT	1 cut(s)	398
Psp5II	RGGWCCY	1 cut(s)	510
Psp6I	CCWGG	9 cut(s)	62, 424, 814, 898, 928, 954, 1083, 1189, 1296
PspFI	CCCAGC	1 cut(s)	299
PspGI	CCWGG	9 cut(s)	62, 424, 814, 898, 928, 954, 1083, 1189, 1296
PspN4I	GGNNCC	4 cut(s)	511, 512, 1225, 1306
PspPI	GGNCC	5 cut(s)	6, 319, 452, 510, 1265
PspPPI	RGGWCCY	1 cut(s)	510
PspXI	VCTCGAGB	1 cut(s)	246
PsrI	GAACNNNNNNTAC	2 cut(s)	164, 196
PstNI	CAGNNNCTG	2 cut(s)	449, 1178
PsyI	GACNNNGTC	1 cut(s)	1079
RsaI	GTAC	5 cut(s)	60, 582, 1092, 1137, 1243
RsaNI	GTAC	5 cut(s)	59, 581, 1091, 1136, 1242
RseI	CAYNNNNRTG	1 cut(s)	1007
SaqAI	TTAA	6 cut(s)	228, 570, 659, 687, 696, 1346
SatI	GCNGC	5 cut(s)	19, 565, 876, 1029, 1049
Sau3AI	GATC	3 cut(s)	254, 944, 1209
Sau96I	GGNCC	5 cut(s)	6, 319, 452, 510, 1265
SchI	GAGTC	4 cut(s)	122, 158, 488, 1309
ScrFI	CCNGG	9 cut(s)	64, 426, 816, 900, 930, 956, 1085, 1191, 1298
SduI	GDGCHC	1 cut(s)	1004
SfaNI	GCATC	3 cut(s)	263, 887, 1208
SfcI	CTRYAG	2 cut(s)	357, 825
Sfr274I	CTCGAG	1 cut(s)	246
SinI	GGWCC	4 cut(s)	319, 452, 510, 1265
SlaI	CTCGAG	1 cut(s)	246
SmiMI	CAYNNNNRTG	1 cut(s)	1007
SmlI	CTYRAG	2 cut(s)	79, 246
SmoI	CTYRAG	2 cut(s)	79, 246
SpeI	ACTAGT	1 cut(s)	1327
Sse9I	AATT	5 cut(s)	196, 336, 684, 693, 746
SsiI	CCGC	3 cut(s)	18, 564, 794
SspMI	CTAG	1 cut(s)	1328
StyD4I	CCNGG	9 cut(s)	62, 424, 814, 898, 928, 954, 1083, 1189, 1296
StyI	CCWWGG	2 cut(s)	468, 904
TaaI	ACNGT	3 cut(s)	372, 737, 1081
TaiI	ACGT	2 cut(s)	26, 310
TaqI	TCGA	2 cut(s)	247, 1233
TasI	AATT	5 cut(s)	196, 336, 684, 693, 746
TatI	WGTACW	3 cut(s)	580, 1135, 1241
TauI	GCSGC	2 cut(s)	21, 567
TfiI	GAWTC	2 cut(s)	350, 706
Tru1I	TTAA	6 cut(s)	228, 570, 659, 687, 696, 1346
Tru9I	TTAA	6 cut(s)	228, 570, 659, 687, 696, 1346
TscAI	CASTG	6 cut(s)	165, 283, 1158, 1179, 1275, 1325
TseFI	GTSAC	1 cut(s)	932
TseI	GCWGC	3 cut(s)	875, 1028, 1048
Tsp45I	GTSAC	1 cut(s)	932
TspDTI	ATGAA	6 cut(s)	107, 309, 633, 1149, 1219, 1245
TspRI	CASTG	6 cut(s)	165, 283, 1158, 1179, 1275, 1325
Tth111I	GACNNNGTC	1 cut(s)	1079
Van91I	CCANNNNNTGG	2 cut(s)	839, 1178
VpaK11BI	GGWCC	4 cut(s)	319, 452, 510, 1265
XapI	RAATTY	1 cut(s)	746
XceI	RCATGY	1 cut(s)	402
XhoI	CTCGAG	1 cut(s)	246
XspI	CTAG	1 cut(s)	1328
Zsp2I	ATGCAT	1 cut(s)	1201

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.