pycom03g16940

Methionine gamma-lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0015573
Physical Location & Seq
Reverse (-)
18104541 .. 18108975
4435 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g16940.9

Sequence Viewer

Length: 1359 bp
ATGTGGGCCTTATCCTGCGCCACGTCTGCTTATCCTACGCTCACTGCCAGAGCTTCCGTACCCAGGGCTACTAATCGCTCAAGCTCTCCACTCTCAGTCCCTACCAATCACCATTTTCATGCGAAAGACTCTCCTTTTGTTCCAGAGATTTCGGACGCAGTGGACTCGTTGTATTCAGAGTTCAGAGCCGTGGATAATTTGGTTGCACGCAATACCACCCGTGTTCTTAAAGCTTTCCAGAATGCTCGAGTTGGATCACATCACTTTGCCGGATGCACTGGTTATGGTCATGATGAAGCTGGGGGACGTGAAGCGCTAGACCAAGCTTTTGCTGAAATTGTTGGGGCTGAATCTGCTATAGTTCGCTCACAGTTTTTTTCAGGAACTCATGCTATAACGTGTGCTTTATTTGCTTTTCTAAGGCCAGGGGATGAGCTTTTGGCAGTTGCTGGTCCTCCCTATGACACCTTGGAGGAAGTTATTGGAAAGAGAGACTCTCATGGCATGGGGTCCTTGACAGCTTTTGGAGTGAAATACCGAGAAGTTCCACTTGCTGAAGATGGCGGCCTTAACTGGGATGCACTTATCCATGCTTTGAGACCTGAAACAAAATGTGCGCTCATACAGAGGTCATGTGGTTATTCATGGCGTCGGAGTTTAAGTGTAGATGATATAGGGCAAGCAATTAAGATAATTAAGACGCAGAATCCTAACTGCTTGGTCATGGTGGATAACTGTTATGGTGAATTTGTGGAAAGCATTGAACCTCCATTGGTGGGTGCAGATTTGATTGCGGGCAGTTTGATAAAAAATCCTGGTGGGACTATAGCACCATGTGGTGGATATGTTGCTGGGAAAGAAAAATGGGTAAAAGCAGCATCAGCTCGTCTGTCTGCACCTGGCCTTGGAGTTGATTGTGGCGCAACTCCTGGTGACATTATGCGAGCATTTTTCCAGGGTTTGTTCCTTTCACCTCAAATGGTAGGCGAGGCTATCAAGGGCACTTTACTGGTAGCTGAAGTTATGGCAGCCAGAGGGTATAAGGTGCAGCCACTTCCTCGTATTCCTCGTCATGACACAGTCCAGGCTGTACAGCTTGGAAGCCGTGAGCGCCTTCTTGCTTTCTGCGAGGCTGTACAGAGAAACTCCCCAGTAGGTTCATTTACCAAACCAGTGGCTGGTGCAACTCCTGGATATGCATCAGAGGTGATCTTTGCTGATGGAACCTTCATTGATGGGAGTACAAGTGAGCTTTCATGCGATGGACCATTAAGAGAGCCATTTGCCGTGTTTTGCCAGGGTGGCTCCCATTGGACTCAGTGGGGACTAGTTCTTGGAGAGGTTTTAAAAGCCATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003962 GO:0004123 GO:0016740 GO:0016765 GO:0016829 GO:0016846
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Met_gamma_lyase

Protein Analysis

453

Amino Acids

48.17

Weight (kDa)

6.14

Isoelectric Point (pI)

31.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015573)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g20850 FvH4_3g20850 FvH4_3g20850
malus_domestica MD03G1220100.v1.1
prunus_persica Prupe.4G188800_v2.0.a1 Prupe.4G188800_v2.0.a1
pyrus_communis pycom03g16940
rosa_chinensis RchiOBHm_Chr5g0035161
rosa_laevigata RLG00000033603
rosa_multiflora Rmu_sc0000879.1_g000001 Rmu_sc0008176.1_g000007
rosa_roxburghii Rroxscaffold_1G00045250
rosa_rugosa Rorug05G0150400
rosa_samantha Rh5BG242800 Rh5DG251600
rosa_wichuraiana Rw5G022220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 839, 1178
AciI CCGC 2 cut(s) 564, 794
AclWI GGATC 1 cut(s) 262
AcsI RAATTY 1 cut(s) 746
AcuI CTGAAG 2 cut(s) 576, 1038
AcyI GRCGYC 1 cut(s) 649
AdeI CACNNNGTG 1 cut(s) 836
AfaI GTAC 4 cut(s) 60, 1092, 1137, 1243
AfeI AGCGCT 1 cut(s) 315
AfiI CCNNNNNNNGG 6 cut(s) 63, 574, 776, 839, 905, 1178
AflIII ACRYGT 1 cut(s) 398
AgsI TTSAA 1 cut(s) 764
AhlI ACTAGT 1 cut(s) 1327
AjiI CACGTC 2 cut(s) 24, 308
AjnI CCWGG 9 cut(s) 62, 424, 814, 898, 928, 954, 1083, 1189, 1296
Alw26I GTCTC 2 cut(s) 486, 592
AlwI GGATC 1 cut(s) 262
AlwNI CAGNNNCTG 2 cut(s) 449, 1178
Ama87I CYCGRG 1 cut(s) 246
Aor51HI AGCGCT 1 cut(s) 315
AoxI GGCC 4 cut(s) 6, 422, 565, 901
ApeKI GCWGC 3 cut(s) 875, 1028, 1048
ApoI RAATTY 1 cut(s) 746
ArsI GACNNNNNNTTYG 4 cut(s) 119, 151, 311, 343
AspLEI GCGC 5 cut(s) 20, 316, 619, 923, 1113
AspS9I GGNCC 4 cut(s) 6, 452, 510, 1265
AsuHPI GGTGA 5 cut(s) 101, 755, 944, 963, 1219
AvaI CYCGRG 1 cut(s) 246
AvaII GGWCC 3 cut(s) 452, 510, 1265
BaeGI GKGCMC 1 cut(s) 1004
BbvI GCAGC 3 cut(s) 887, 1040, 1060
BccI CCATC 4 cut(s) 554, 1214, 1229, 1256
BceAI ACGGC 3 cut(s) 173, 1089, 1271
BcgI CGANNNNNNTGC 2 cut(s) 147, 181
BciT130I CCWGG 9 cut(s) 64, 426, 816, 900, 930, 956, 1085, 1191, 1298
BcoDI GTCTC 2 cut(s) 486, 592
BcuI ACTAGT 1 cut(s) 1327
BfaI CTAG 2 cut(s) 317, 1328
BfmI CTRYAG 2 cut(s) 357, 825
BfoI RGCGCY 2 cut(s) 317, 1114
BglI GCCNNNNNGGC 1 cut(s) 1302
BisI GCNGC 4 cut(s) 565, 876, 1029, 1049
BlsI GCNGC 4 cut(s) 566, 877, 1030, 1050
Bme1390I CCNGG 9 cut(s) 64, 426, 816, 900, 930, 956, 1085, 1191, 1298
Bme18I GGWCC 3 cut(s) 452, 510, 1265
BmeT110I CYCGRG 1 cut(s) 246
BmgBI CACGTC 2 cut(s) 24, 308
BmgT120I GGNCC 4 cut(s) 6, 452, 510, 1265
BmiI GGNNCC 3 cut(s) 511, 1225, 1306
BmrFI CCNGG 9 cut(s) 64, 426, 816, 900, 930, 956, 1085, 1191, 1298
BmrI ACTGGG 2 cut(s) 583, 1145
BmsI GCATC 4 cut(s) 263, 568, 887, 1208
BmuI ACTGGG 2 cut(s) 583, 1145
BplI GAGNNNNNCTC 2 cut(s) 481, 513
BpuEI CTTGAG 1 cut(s) 64
BsaBI GATNNNNATC 1 cut(s) 1198
BsaHI GRCGYC 1 cut(s) 649
BsaI GGTCTC 1 cut(s) 592
BsaJI CCNNGG 8 cut(s) 62, 63, 189, 425, 468, 904, 955, 1297
BsaXI ACNNNNNCTCC 4 cut(s) 646, 676, 1231, 1261
Bsc4I CCNNNNNNNGG 6 cut(s) 63, 574, 776, 839, 905, 1178
Bse1I ACTGG 5 cut(s) 283, 578, 1014, 1151, 1172
Bse8I GATNNNNATC 1 cut(s) 1198
BseBI CCWGG 9 cut(s) 64, 426, 816, 900, 930, 956, 1085, 1191, 1298
BseDI CCNNGG 8 cut(s) 62, 63, 189, 425, 468, 904, 955, 1297
BseGI GGATG 3 cut(s) 278, 436, 583
BseJI GATNNNNATC 1 cut(s) 1198
BseLI CCNNNNNNNGG 6 cut(s) 63, 574, 776, 839, 905, 1178
BseMII CTCAG 2 cut(s) 108, 1331
BseNI ACTGG 5 cut(s) 283, 578, 1014, 1151, 1172
BseSI GKGCMC 1 cut(s) 1004
BseXI GCAGC 3 cut(s) 887, 1040, 1060
BseYI CCCAGC 2 cut(s) 299, 851
BsgI GTGCAG 3 cut(s) 801, 879, 1067
BshFI GGCC 4 cut(s) 8, 424, 567, 903
BsiHKCI CYCGRG 1 cut(s) 246
BsiSI CCGG 1 cut(s) 270
BslFI GGGAC 4 cut(s) 83, 318, 835, 1338
BslI CCNNNNNNNGG 6 cut(s) 63, 574, 776, 839, 905, 1178
BsmAI GTCTC 2 cut(s) 486, 592
BsmFI GGGAC 4 cut(s) 83, 318, 835, 1338
BsmI GAATGC 1 cut(s) 247
BsnI GGCC 4 cut(s) 8, 424, 567, 903
Bso31I GGTCTC 1 cut(s) 592
BsoBI CYCGRG 1 cut(s) 246
Bsp1286I GDGCHC 1 cut(s) 1004
Bsp1407I TGTACA 2 cut(s) 1090, 1135
Bsp143I GATC 2 cut(s) 254, 1209
BspACI CCGC 2 cut(s) 564, 794
BspANI GGCC 4 cut(s) 8, 424, 567, 903
BspCNI CTCAG 2 cut(s) 107, 1330
BspHI TCATGA 2 cut(s) 289, 1072
BspLI GGNNCC 3 cut(s) 511, 1225, 1306
BspPI GGATC 1 cut(s) 262
BspTNI GGTCTC 1 cut(s) 592
BsrGI TGTACA 2 cut(s) 1090, 1135
BsrI ACTGG 5 cut(s) 283, 578, 1014, 1151, 1172
BssECI CCNNGG 8 cut(s) 62, 63, 189, 425, 468, 904, 955, 1297
BssMI GATC 2 cut(s) 254, 1209
BssNI GRCGYC 1 cut(s) 649
BssT1I CCWWGG 2 cut(s) 468, 904
Bst2UI CCWGG 9 cut(s) 64, 426, 816, 900, 930, 956, 1085, 1191, 1298
Bst4CI ACNGT 3 cut(s) 372, 737, 1081
BstACI GRCGYC 1 cut(s) 649
BstAUI TGTACA 2 cut(s) 1090, 1135
BstC8I GCNNGC 4 cut(s) 208, 681, 796, 945
BstDEI CTNAG 3 cut(s) 94, 419, 1317
BstDSI CCRYGG 1 cut(s) 189
BstF5I GGATG 3 cut(s) 278, 436, 583
BstH2I RGCGCY 2 cut(s) 317, 1114
BstHHI GCGC 5 cut(s) 20, 316, 619, 923, 1113
BstKTI GATC 2 cut(s) 257, 1212
BstMAI GTCTC 2 cut(s) 486, 592
BstMBI GATC 2 cut(s) 254, 1209
BstMWI GCNNNNNNNGC 6 cut(s) 26, 353, 410, 881, 1110, 1302
BstNI CCWGG 9 cut(s) 64, 426, 816, 900, 930, 956, 1085, 1191, 1298
BstSCI CCNGG 9 cut(s) 62, 424, 814, 898, 928, 954, 1083, 1189, 1296
BstSFI CTRYAG 2 cut(s) 357, 825
BstSLI GKGCMC 1 cut(s) 1004
BstV1I GCAGC 3 cut(s) 887, 1040, 1060
BstXI CCANNNNNNTGG 1 cut(s) 1174
BsuRI GGCC 4 cut(s) 8, 424, 567, 903
BtgI CCRYGG 1 cut(s) 189
BtgZI GCGATG 1 cut(s) 1275
BtrI CACGTC 2 cut(s) 24, 308
BtsCI GGATG 3 cut(s) 278, 436, 583
BtsI GCAGTG 2 cut(s) 42, 165
BtsIMutI CAGTG 5 cut(s) 42, 165, 276, 1179, 1325
Cac8I GCNNGC 4 cut(s) 208, 681, 796, 945
CaiI CAGNNNCTG 2 cut(s) 449, 1178
CciI TCATGA 2 cut(s) 289, 1072
CfoI GCGC 5 cut(s) 20, 316, 619, 923, 1113
Cfr13I GGNCC 4 cut(s) 6, 452, 510, 1265
CseI GACGC 3 cut(s) 164, 638, 709
Csp6I GTAC 4 cut(s) 59, 1091, 1136, 1242
CviQI GTAC 4 cut(s) 59, 1091, 1136, 1242
DdeI CTNAG 3 cut(s) 94, 419, 1317
DpnI GATC 2 cut(s) 256, 1211
DpnII GATC 2 cut(s) 254, 1209
DraI TTTAAA 1 cut(s) 1347
DraIII CACNNNGTG 1 cut(s) 836
Eco130I CCWWGG 2 cut(s) 468, 904
Eco31I GGTCTC 1 cut(s) 592
Eco47I GGWCC 3 cut(s) 452, 510, 1265
Eco47III AGCGCT 1 cut(s) 315
Eco57I CTGAAG 2 cut(s) 576, 1038
Eco88I CYCGRG 1 cut(s) 246
EcoO109I RGGNCCY 1 cut(s) 510
EcoRII CCWGG 9 cut(s) 62, 424, 814, 898, 928, 954, 1083, 1189, 1296
EcoT14I CCWWGG 2 cut(s) 468, 904
EcoT22I ATGCAT 1 cut(s) 1201
ErhI CCWWGG 2 cut(s) 468, 904
FalI AAGNNNNNCTT 2 cut(s) 534, 566
FaqI GGGAC 4 cut(s) 83, 318, 835, 1338
FauI CCCGC 1 cut(s) 787
Fnu4HI GCNGC 4 cut(s) 565, 876, 1029, 1049
FokI GGATG 3 cut(s) 285, 443, 590
Fsp4HI GCNGC 4 cut(s) 565, 876, 1029, 1049
FspBI CTAG 2 cut(s) 317, 1328
GlaI GCGC 5 cut(s) 19, 315, 618, 922, 1112
GluI GCNGC 4 cut(s) 565, 876, 1029, 1049
GsaI CCCAGC 2 cut(s) 303, 855
HaeII RGCGCY 2 cut(s) 317, 1114
HaeIII GGCC 4 cut(s) 8, 424, 567, 903
HapII CCGG 1 cut(s) 270
HgaI GACGC 3 cut(s) 164, 638, 709
HhaI GCGC 5 cut(s) 20, 316, 619, 923, 1113
Hin1I GRCGYC 1 cut(s) 649
Hin6I GCGC 5 cut(s) 18, 314, 617, 921, 1111
HinP1I GCGC 5 cut(s) 18, 314, 617, 921, 1111
HindIII AAGCTT 2 cut(s) 231, 324
HinfI GANTC 6 cut(s) 128, 164, 350, 494, 706, 1315
HpaII CCGG 1 cut(s) 270
HphI GGTGA 5 cut(s) 101, 755, 944, 963, 1219
Hpy166II GTNNAC 1 cut(s) 163
Hpy188I TCNGA 5 cut(s) 154, 178, 185, 654, 1204
Hpy188III TCNNGA 5 cut(s) 143, 238, 290, 381, 1073
Hpy8I GTNNAC 1 cut(s) 163
Hpy99I CGWCG 1 cut(s) 654
HpyAV CCTTC 2 cut(s) 1124, 1237
HpyCH4III ACNGT 3 cut(s) 372, 737, 1081
HpyCH4IV ACGT 3 cut(s) 23, 307, 398
HpyCH4V TGCA 8 cut(s) 206, 276, 581, 782, 896, 1048, 1184, 1199
HpyF10VI GCNNNNNNNGC 6 cut(s) 26, 353, 410, 881, 1110, 1302
HpyF3I CTNAG 3 cut(s) 94, 419, 1317
HpySE526I ACGT 3 cut(s) 23, 307, 398
Hsp92I GRCGYC 1 cut(s) 649
HspAI GCGC 5 cut(s) 18, 314, 617, 921, 1111
Kzo9I GATC 2 cut(s) 254, 1209
LmnI GCTCC 1 cut(s) 1310
Lsp1109I GCAGC 3 cut(s) 887, 1040, 1060
LweI GCATC 4 cut(s) 263, 568, 887, 1208
MaeI CTAG 2 cut(s) 317, 1328
MaeII ACGT 3 cut(s) 23, 307, 398
MaeIII GTNAC 1 cut(s) 932
MalI GATC 2 cut(s) 256, 1211
MboI GATC 2 cut(s) 254, 1209
MboII GAAGA 1 cut(s) 569
MhlI GDGCHC 1 cut(s) 1004
MluCI AATT 5 cut(s) 196, 336, 684, 693, 746
MlyI GAGTC 4 cut(s) 122, 158, 488, 1309
MmeI TCCRAC 2 cut(s) 232, 632
Mph1103I ATGCAT 1 cut(s) 1201
MseI TTAA 7 cut(s) 228, 570, 659, 687, 696, 1271, 1346
MslI CAYNNNNRTG 1 cut(s) 117
MspI CCGG 1 cut(s) 270
MspR9I CCNGG 9 cut(s) 64, 426, 816, 900, 930, 956, 1085, 1191, 1298
Mva1269I GAATGC 1 cut(s) 247
MvaI CCWGG 9 cut(s) 64, 426, 816, 900, 930, 956, 1085, 1191, 1298
MwoI GCNNNNNNNGC 6 cut(s) 26, 353, 410, 881, 1110, 1302
NdeII GATC 2 cut(s) 254, 1209
NlaIV GGNNCC 3 cut(s) 511, 1225, 1306
NmuCI GTSAC 1 cut(s) 932
NsiI ATGCAT 1 cut(s) 1201
PaeR7I CTCGAG 1 cut(s) 246
PagI TCATGA 2 cut(s) 289, 1072
PasI CCCWGGG 1 cut(s) 63
PcsI WCGNNNNNNNCGW 1 cut(s) 1066
PctI GAATGC 1 cut(s) 247
PfeI GAWTC 2 cut(s) 350, 706
PflFI GACNNNGTC 1 cut(s) 1079
PflMI CCANNNNNTGG 2 cut(s) 839, 1178
PfoI TCCNGGA 1 cut(s) 1189
PkrI GCNGC 4 cut(s) 566, 877, 1030, 1050
PleI GAGTC 4 cut(s) 122, 158, 488, 1309
PpsI GAGTC 4 cut(s) 122, 158, 488, 1309
PpuMI RGGWCCY 1 cut(s) 510
Psp5II RGGWCCY 1 cut(s) 510
Psp6I CCWGG 9 cut(s) 62, 424, 814, 898, 928, 954, 1083, 1189, 1296
PspFI CCCAGC 2 cut(s) 299, 851
PspGI CCWGG 9 cut(s) 62, 424, 814, 898, 928, 954, 1083, 1189, 1296
PspN4I GGNNCC 3 cut(s) 511, 1225, 1306
PspPI GGNCC 4 cut(s) 6, 452, 510, 1265
PspPPI RGGWCCY 1 cut(s) 510
PspXI VCTCGAGB 1 cut(s) 246
PsrI GAACNNNNNNTAC 2 cut(s) 164, 196
PstNI CAGNNNCTG 2 cut(s) 449, 1178
PsyI GACNNNGTC 1 cut(s) 1079
RsaI GTAC 4 cut(s) 60, 1092, 1137, 1243
RsaNI GTAC 4 cut(s) 59, 1091, 1136, 1242
RseI CAYNNNNRTG 1 cut(s) 117
SaqAI TTAA 7 cut(s) 228, 570, 659, 687, 696, 1271, 1346
SatI GCNGC 4 cut(s) 565, 876, 1029, 1049
Sau3AI GATC 2 cut(s) 254, 1209
Sau96I GGNCC 4 cut(s) 6, 452, 510, 1265
SchI GAGTC 4 cut(s) 122, 158, 488, 1309
ScrFI CCNGG 9 cut(s) 64, 426, 816, 900, 930, 956, 1085, 1191, 1298
SduI GDGCHC 1 cut(s) 1004
SfaNI GCATC 4 cut(s) 263, 568, 887, 1208
SfcI CTRYAG 2 cut(s) 357, 825
Sfr274I CTCGAG 1 cut(s) 246
SinI GGWCC 3 cut(s) 452, 510, 1265
SlaI CTCGAG 1 cut(s) 246
SmiMI CAYNNNNRTG 1 cut(s) 117
SmlI CTYRAG 2 cut(s) 79, 246
SmoI CTYRAG 2 cut(s) 79, 246
SpeI ACTAGT 1 cut(s) 1327
Sse9I AATT 5 cut(s) 196, 336, 684, 693, 746
SsiI CCGC 2 cut(s) 564, 794
SspMI CTAG 2 cut(s) 317, 1328
StyD4I CCNGG 9 cut(s) 62, 424, 814, 898, 928, 954, 1083, 1189, 1296
StyI CCWWGG 2 cut(s) 468, 904
TaaI ACNGT 3 cut(s) 372, 737, 1081
TaiI ACGT 3 cut(s) 26, 310, 401
TaqI TCGA 1 cut(s) 247
TasI AATT 5 cut(s) 196, 336, 684, 693, 746
TatI WGTACW 3 cut(s) 1090, 1135, 1241
TauI GCSGC 1 cut(s) 567
TfiI GAWTC 2 cut(s) 350, 706
Tru1I TTAA 7 cut(s) 228, 570, 659, 687, 696, 1271, 1346
Tru9I TTAA 7 cut(s) 228, 570, 659, 687, 696, 1271, 1346
TscAI CASTG 5 cut(s) 49, 165, 283, 1179, 1325
TseFI GTSAC 1 cut(s) 932
TseI GCWGC 3 cut(s) 875, 1028, 1048
Tsp45I GTSAC 1 cut(s) 932
TspDTI ATGAA 6 cut(s) 107, 309, 633, 1149, 1219, 1245
TspGWI ACGGA 1 cut(s) 46
TspRI CASTG 5 cut(s) 49, 165, 283, 1179, 1325
Tth111I GACNNNGTC 1 cut(s) 1079
Van91I CCANNNNNTGG 2 cut(s) 839, 1178
VpaK11BI GGWCC 3 cut(s) 452, 510, 1265
XapI RAATTY 1 cut(s) 746
XhoI CTCGAG 1 cut(s) 246
XspI CTAG 2 cut(s) 317, 1328
Zsp2I ATGCAT 1 cut(s) 1201
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.