RchiOBHm_Chr5g0035161

Methionine gamma-lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0015573
Physical Location & Seq
Forward (+)
29050492 .. 29055344
4853 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ31408

Sequence Viewer

Length: 1365 bp
ATGTGGGCCTTATCCTGCGCAACCTCTGCTTATCCTGCGCTCACTTTGAGAGCGTCTGTACCCATGGCCACTACTCGTTCCGGCTCTCCCGTCTCCGTCTCAGTCCCTACCAATCACCATTGTTATCAGAAAGACAGCCCTTTTGTTCCTGAGGTTGTGGAAGCAGTGGATTCCTTGCATTCAGAGTTCAGAGCTGTGGATAATTTGGTAGCACGCAATACCACCCGGGTTCTTAAAGCTTTCCAGAATGCCCGAATTGGATCTCATCACTTTGCTGGAAGTACTGGCTATGGTCACGATGAAGCTGGGGGGCGTGAAGCTCTTGACCAAGCTTTTGCAGAAATTGTAGGCGCTGAATCTGCAGTAGTTCGCTCACAGTTTTTCTCAGGAACTCATGCTATAACCTGCGCTCTGTTTGCTTTTCTAAGGCCAGGAGATGAGCTTTTGGCAGTTGCTGGTCCTCCCTATGACACCTTGGAGGAGGTTATTGGAAAGAGAGACTCTCATGGCATGGGATCCTTGATAGACTTTGGAGTGAAATACCGTGAAGTTCCACTTGCTGAAGATGGTGGCCTTGACTGGGATGCGCTTAGGCATGCTTTGAACCCTGAAACAAAATGTGCACTCATACAGCGGTCATGTGGTTATTCATGGCGTCAGAGTTTAAGTGTGGATGATATAAGGCGAGCAATTGAGATAATTAAGATGCAGAATCCTAACTGCCTGGTCATGGTGGATAATTGTTATGGTGAATTTGTGGAAAGCATTGAACCTCCAATGGTGGGTGCAGATTTAATTGCTGGCAGTTTGATAAAAAATCCTGGTGGAACTATAGCCCCATGCGGTGGATATGTTGCAGGAAGAGAAAGATGGGTAAAAGCAGCAGCAGCTCGTCTGTCTGCACCTGGCCTTGGAGTTGATTGTGGCTCAACTCCTGGTCACATTATGAGAGCATTTTTCCAAGGTTTGTTCCTTTCACCTCAAATGGTTGGCGAGGCTATCAAGGGGACTTTCCTAGTAGCTGAAGTTATGGCTGCCAAAGGGTATAAGGTCCAGCCCCTTCCTCGTGTTCCTCGCCATGACACAGTACAGGCCATACAGCTTGGAAATCGTGAGCGTCTTCTTGCTTTCTGTGAGGCTGTGCAGAGGAGCTCCCCAGTCGGTTCATTTACTAAACCAATTGCTGGCACAACTCCTGGATATGCATCAGAGGTGATCTTTGCTGATGGAACATTTGTTGATGGGAGTACAAGTGAGCTTTCATGCGATGGACCATTAAGAGAGCCATTCGCTGTATTTTGCCAGGGTGGCTCCCATTGGACTCAGTGGGGACTAGTTCTTGGAGAGGTTTTAAAAGCCATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003962 GO:0004123 GO:0016740 GO:0016765 GO:0016829 GO:0016846
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Met_gamma_lyase

Protein Analysis

454

Amino Acids

48.62

Weight (kDa)

5.86

Isoelectric Point (pI)

36.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Met_gamma_lyase PF06838 54 - 452 2.4e-164 Methionine gamma-lyase
Cys_Met_Meta_PP PF01053 61 - 297 2.2e-09 Cys/Met metabolism PLP-dependent enzyme
Aminotran_1_2 PF00155 101 - 308 8.5e-06 Aminotransferase class I and II
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015573)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g20850 FvH4_3g20850 FvH4_3g20850
malus_domestica MD03G1220100.v1.1
prunus_persica Prupe.4G188800_v2.0.a1 Prupe.4G188800_v2.0.a1
pyrus_communis pycom03g16940
rosa_chinensis RchiOBHm_Chr5g0035161
rosa_laevigata RLG00000033603
rosa_multiflora Rmu_sc0000879.1_g000001 Rmu_sc0008176.1_g000007
rosa_roxburghii Rroxscaffold_1G00045250
rosa_rugosa Rorug05G0150400
rosa_samantha Rh5BG242800 Rh5DG251600
rosa_wichuraiana Rw5G022220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 19
Acc36I ACCTGC 1 cut(s) 413
AccB7I CCANNNNNTGG 2 cut(s) 845, 1184
AciI CCGC 2 cut(s) 634, 843
AclWI GGATC 3 cut(s) 268, 510, 523
AcoI YGGCCR 1 cut(s) 66
AcsI RAATTY 1 cut(s) 752
AcuI CTGAAG 2 cut(s) 582, 1044
AcyI GRCGYC 1 cut(s) 655
AfaI GTAC 4 cut(s) 60, 283, 1089, 1249
AfiI CCNNNNNNNGG 6 cut(s) 580, 730, 782, 845, 911, 1184
AgsI TTSAA 2 cut(s) 604, 770
AhlI ACTAGT 1 cut(s) 1333
AjnI CCWGG 7 cut(s) 430, 723, 820, 904, 934, 1195, 1302
Alw21I GWGCWC 2 cut(s) 625, 1154
Alw26I GTCTC 3 cut(s) 97, 103, 492
Alw44I GTGCAC 1 cut(s) 621
AlwI GGATC 3 cut(s) 268, 510, 523
AlwNI CAGNNNCTG 1 cut(s) 455
Ama87I CYCGRG 1 cut(s) 225
AoxI GGCC 6 cut(s) 6, 66, 428, 571, 907, 1092
ApaLI GTGCAC 1 cut(s) 621
ApeKI GCWGC 4 cut(s) 881, 884, 887, 1034
ApoI RAATTY 1 cut(s) 752
ArsI GACNNNNNNTTYG 4 cut(s) 125, 157, 317, 349
AspLEI GCGC 5 cut(s) 20, 40, 353, 410, 589
AspS9I GGNCC 4 cut(s) 6, 458, 1051, 1271
AsuC2I CCSGG 2 cut(s) 226, 227
AsuHPI GGTGA 4 cut(s) 107, 761, 969, 1225
AvaI CYCGRG 1 cut(s) 225
AvaII GGWCC 3 cut(s) 458, 1051, 1271
AxyI CCTNAGG 1 cut(s) 150
BaeGI GKGCMC 1 cut(s) 625
BalI TGGCCA 1 cut(s) 68
BamHI GGATCC 1 cut(s) 515
BanII GRGCYC 1 cut(s) 1154
BauI CACGAG 1 cut(s) 1065
BbsI GAAGAC 1 cut(s) 1112
Bbv12I GWGCWC 2 cut(s) 625, 1154
BbvI GCAGC 4 cut(s) 893, 896, 899, 1021
BccI CCATC 5 cut(s) 560, 864, 1220, 1235, 1262
BciT130I CCWGG 7 cut(s) 432, 725, 822, 906, 936, 1197, 1304
BcnI CCSGG 2 cut(s) 226, 227
BcoDI GTCTC 3 cut(s) 97, 103, 492
BcuI ACTAGT 1 cut(s) 1333
BfaI CTAG 2 cut(s) 1016, 1334
BfmI CTRYAG 2 cut(s) 360, 831
BfoI RGCGCY 1 cut(s) 354
BfuAI ACCTGC 1 cut(s) 413
BglI GCCNNNNNGGC 1 cut(s) 1308
BisI GCNGC 4 cut(s) 882, 885, 888, 1035
BlsI GCNGC 4 cut(s) 883, 886, 889, 1036
BmcAI AGTACT 1 cut(s) 283
Bme1390I CCNGG 9 cut(s) 226, 227, 432, 725, 822, 906, 936, 1197, 1304
Bme18I GGWCC 3 cut(s) 458, 1051, 1271
BmeT110I CYCGRG 1 cut(s) 225
BmgT120I GGNCC 4 cut(s) 6, 458, 1051, 1271
BmiI GGNNCC 2 cut(s) 517, 1312
BmrFI CCNGG 9 cut(s) 226, 227, 432, 725, 822, 906, 936, 1197, 1304
BmrI ACTGGG 2 cut(s) 589, 1151
BmsI GCATC 3 cut(s) 574, 696, 1214
BmuI ACTGGG 2 cut(s) 589, 1151
BpiI GAAGAC 1 cut(s) 1112
BplI GAGNNNNNCTC 2 cut(s) 487, 519
Bpu10I CCTNAGC 1 cut(s) 590
BpuMI CCSGG 2 cut(s) 226, 227
BsaBI GATNNNNATC 1 cut(s) 1204
BsaHI GRCGYC 1 cut(s) 655
BsaJI CCNNGG 6 cut(s) 63, 225, 474, 910, 961, 1303
BsaXI ACNNNNNCTCC 2 cut(s) 1237, 1267
Bsc4I CCNNNNNNNGG 6 cut(s) 580, 730, 782, 845, 911, 1184
Bse1I ACTGG 3 cut(s) 289, 584, 1157
Bse21I CCTNAGG 1 cut(s) 150
Bse8I GATNNNNATC 1 cut(s) 1204
BseBI CCWGG 7 cut(s) 432, 725, 822, 906, 936, 1197, 1304
BseDI CCNNGG 6 cut(s) 63, 225, 474, 910, 961, 1303
BseGI GGATG 2 cut(s) 589, 679
BseJI GATNNNNATC 1 cut(s) 1204
BseLI CCNNNNNNNGG 6 cut(s) 580, 730, 782, 845, 911, 1184
BseMII CTCAG 4 cut(s) 114, 141, 399, 1337
BseNI ACTGG 3 cut(s) 289, 584, 1157
BseRI GAGGAG 2 cut(s) 494, 1162
BseSI GKGCMC 1 cut(s) 625
BseXI GCAGC 4 cut(s) 893, 896, 899, 1021
BseYI CCCAGC 1 cut(s) 305
BsgI GTGCAG 3 cut(s) 807, 885, 1163
BshFI GGCC 6 cut(s) 8, 68, 430, 573, 909, 1094
BsiHKAI GWGCWC 2 cut(s) 625, 1154
BsiHKCI CYCGRG 1 cut(s) 225
BsiSI CCGG 2 cut(s) 81, 226
BslFI GGGAC 3 cut(s) 89, 1021, 1344
BslI CCNNNNNNNGG 6 cut(s) 580, 730, 782, 845, 911, 1184
BsmAI GTCTC 3 cut(s) 97, 103, 492
BsmBI CGTCTC 2 cut(s) 97, 103
BsmFI GGGAC 3 cut(s) 89, 1021, 1344
BsmI GAATGC 2 cut(s) 178, 253
BsnI GGCC 6 cut(s) 8, 68, 430, 573, 909, 1094
BsoBI CYCGRG 1 cut(s) 225
Bsp1286I GDGCHC 2 cut(s) 625, 1154
Bsp143I GATC 3 cut(s) 260, 515, 1215
Bsp19I CCATGG 1 cut(s) 63
BspACI CCGC 2 cut(s) 634, 843
BspANI GGCC 6 cut(s) 8, 68, 430, 573, 909, 1094
BspCNI CTCAG 4 cut(s) 113, 142, 398, 1336
BspLI GGNNCC 2 cut(s) 517, 1312
BspMAI CTGCAG 1 cut(s) 364
BspMI ACCTGC 1 cut(s) 413
BspPI GGATC 3 cut(s) 268, 510, 523
BsrI ACTGG 3 cut(s) 289, 584, 1157
BssECI CCNNGG 6 cut(s) 63, 225, 474, 910, 961, 1303
BssMI GATC 3 cut(s) 260, 515, 1215
BssNI GRCGYC 1 cut(s) 655
BssSI CACGAG 1 cut(s) 1065
BssT1I CCWWGG 4 cut(s) 63, 474, 910, 961
Bst2BI CACGAG 1 cut(s) 1065
Bst2UI CCWGG 7 cut(s) 432, 725, 822, 906, 936, 1197, 1304
Bst4CI ACNGT 3 cut(s) 378, 545, 1087
Bst6I CTCTTC 1 cut(s) 856
BstACI GRCGYC 1 cut(s) 655
BstAPI GCANNNNNTGC 1 cut(s) 26
BstC8I GCNNGC 5 cut(s) 214, 597, 687, 802, 1186
BstDEI CTNAG 6 cut(s) 100, 150, 385, 425, 590, 1323
BstDSI CCRYGG 1 cut(s) 63
BstF5I GGATG 2 cut(s) 589, 679
BstH2I RGCGCY 1 cut(s) 354
BstHHI GCGC 5 cut(s) 20, 40, 353, 410, 589
BstKTI GATC 3 cut(s) 263, 518, 1218
BstMAI GTCTC 3 cut(s) 97, 103, 492
BstMBI GATC 3 cut(s) 260, 515, 1215
BstMWI GCNNNNNNNGC 6 cut(s) 26, 35, 359, 416, 887, 1308
BstNI CCWGG 7 cut(s) 432, 725, 822, 906, 936, 1197, 1304
BstNSI RCATGY 1 cut(s) 599
BstSCI CCNGG 9 cut(s) 224, 225, 430, 723, 820, 904, 934, 1195, 1302
BstSFI CTRYAG 2 cut(s) 360, 831
BstSLI GKGCMC 1 cut(s) 625
BstV1I GCAGC 4 cut(s) 893, 896, 899, 1021
BstV2I GAAGAC 1 cut(s) 1112
BstX2I RGATCY 2 cut(s) 260, 515
BstYI RGATCY 2 cut(s) 260, 515
Bsu36I CCTNAGG 1 cut(s) 150
BsuRI GGCC 6 cut(s) 8, 68, 430, 573, 909, 1094
BtgI CCRYGG 1 cut(s) 63
BtgZI GCGATG 1 cut(s) 1281
BtsCI GGATG 2 cut(s) 589, 679
BtsI GCAGTG 1 cut(s) 171
BtsIMutI CAGTG 2 cut(s) 171, 1331
BveI ACCTGC 1 cut(s) 413
Cac8I GCNNGC 5 cut(s) 214, 597, 687, 802, 1186
CaiI CAGNNNCTG 1 cut(s) 455
CfoI GCGC 5 cut(s) 20, 40, 353, 410, 589
Cfr13I GGNCC 4 cut(s) 6, 458, 1051, 1271
Cfr9I CCCGGG 1 cut(s) 225
CseI GACGC 3 cut(s) 42, 644, 1106
Csp6I GTAC 4 cut(s) 59, 282, 1088, 1248
CviQI GTAC 4 cut(s) 59, 282, 1088, 1248
DdeI CTNAG 6 cut(s) 100, 150, 385, 425, 590, 1323
DpnI GATC 3 cut(s) 262, 517, 1217
DpnII GATC 3 cut(s) 260, 515, 1215
DraI TTTAAA 1 cut(s) 1353
EaeI YGGCCR 1 cut(s) 66
Eam1104I CTCTTC 1 cut(s) 856
EarI CTCTTC 1 cut(s) 856
Ecl136II GAGCTC 1 cut(s) 1152
Eco130I CCWWGG 4 cut(s) 63, 474, 910, 961
Eco24I GRGCYC 1 cut(s) 1154
Eco47I GGWCC 3 cut(s) 458, 1051, 1271
Eco53kI GAGCTC 1 cut(s) 1152
Eco57I CTGAAG 2 cut(s) 582, 1044
Eco81I CCTNAGG 1 cut(s) 150
Eco88I CYCGRG 1 cut(s) 225
EcoICRI GAGCTC 1 cut(s) 1152
EcoRII CCWGG 7 cut(s) 430, 723, 820, 904, 934, 1195, 1302
EcoT14I CCWWGG 4 cut(s) 63, 474, 910, 961
EcoT22I ATGCAT 1 cut(s) 1207
EcoT38I GRGCYC 1 cut(s) 1154
ErhI CCWWGG 4 cut(s) 63, 474, 910, 961
Esp3I CGTCTC 2 cut(s) 97, 103
FalI AAGNNNNNCTT 2 cut(s) 540, 572
FaqI GGGAC 3 cut(s) 89, 1021, 1344
Fnu4HI GCNGC 4 cut(s) 882, 885, 888, 1035
FokI GGATG 2 cut(s) 596, 686
FriOI GRGCYC 1 cut(s) 1154
Fsp4HI GCNGC 4 cut(s) 882, 885, 888, 1035
FspBI CTAG 2 cut(s) 1016, 1334
FspI TGCGCA 1 cut(s) 19
GlaI GCGC 5 cut(s) 19, 39, 352, 409, 588
GluI GCNGC 4 cut(s) 882, 885, 888, 1035
GsaI CCCAGC 1 cut(s) 309
HaeII RGCGCY 1 cut(s) 354
HaeIII GGCC 6 cut(s) 8, 68, 430, 573, 909, 1094
HapII CCGG 2 cut(s) 81, 226
HgaI GACGC 3 cut(s) 42, 644, 1106
HhaI GCGC 5 cut(s) 20, 40, 353, 410, 589
Hin1I GRCGYC 1 cut(s) 655
Hin6I GCGC 5 cut(s) 18, 38, 351, 408, 587
HinP1I GCGC 5 cut(s) 18, 38, 351, 408, 587
HindIII AAGCTT 2 cut(s) 237, 330
HinfI GANTC 5 cut(s) 170, 356, 500, 712, 1321
HpaII CCGG 2 cut(s) 81, 226
HphI GGTGA 4 cut(s) 107, 761, 969, 1225
Hpy166II GTNNAC 1 cut(s) 623
Hpy188I TCNGA 5 cut(s) 129, 184, 191, 660, 1210
Hpy188III TCNNGA 6 cut(s) 149, 244, 296, 323, 387, 1112
Hpy8I GTNNAC 1 cut(s) 623
HpyAV CCTTC 1 cut(s) 1070
HpyCH4III ACNGT 3 cut(s) 378, 545, 1087
HpyF10VI GCNNNNNNNGC 6 cut(s) 26, 35, 359, 416, 887, 1308
HpyF3I CTNAG 6 cut(s) 100, 150, 385, 425, 590, 1323
Hsp92I GRCGYC 1 cut(s) 655
HspAI GCGC 5 cut(s) 18, 38, 351, 408, 587
Kzo9I GATC 3 cut(s) 260, 515, 1215
LmnI GCTCC 3 cut(s) 1149, 1157, 1316
Lsp1109I GCAGC 4 cut(s) 893, 896, 899, 1021
LweI GCATC 3 cut(s) 574, 696, 1214
MaeI CTAG 2 cut(s) 1016, 1334
MaeIII GTNAC 2 cut(s) 293, 938
MalI GATC 3 cut(s) 262, 517, 1217
MboI GATC 3 cut(s) 260, 515, 1215
MboII GAAGA 3 cut(s) 575, 873, 1112
MfeI CAATTG 2 cut(s) 690, 1179
MflI RGATCY 2 cut(s) 260, 515
MhlI GDGCHC 2 cut(s) 625, 1154
MlsI TGGCCA 1 cut(s) 68
MluCI AATT 9 cut(s) 202, 255, 342, 690, 699, 739, 752, 795, 1179
MluNI TGGCCA 1 cut(s) 68
MlyI GAGTC 2 cut(s) 494, 1315
Mox20I TGGCCA 1 cut(s) 68
Mph1103I ATGCAT 1 cut(s) 1207
MscI TGGCCA 1 cut(s) 68
MseI TTAA 6 cut(s) 234, 665, 702, 794, 1277, 1352
Msp20I TGGCCA 1 cut(s) 68
MspA1I CMGCKG 1 cut(s) 634
MspI CCGG 2 cut(s) 81, 226
MspR9I CCNGG 9 cut(s) 226, 227, 432, 725, 822, 906, 936, 1197, 1304
MunI CAATTG 2 cut(s) 690, 1179
Mva1269I GAATGC 2 cut(s) 178, 253
MvaI CCWGG 7 cut(s) 432, 725, 822, 906, 936, 1197, 1304
MwoI GCNNNNNNNGC 6 cut(s) 26, 35, 359, 416, 887, 1308
NciI CCSGG 2 cut(s) 226, 227
NcoI CCATGG 1 cut(s) 63
NdeII GATC 3 cut(s) 260, 515, 1215
NlaIV GGNNCC 2 cut(s) 517, 1312
NmuCI GTSAC 2 cut(s) 293, 938
NsbI TGCGCA 1 cut(s) 19
NsiI ATGCAT 1 cut(s) 1207
NspI RCATGY 1 cut(s) 599
PaeI GCATGC 1 cut(s) 599
PctI GAATGC 2 cut(s) 178, 253
PfeI GAWTC 3 cut(s) 170, 356, 712
PflMI CCANNNNNTGG 2 cut(s) 845, 1184
PfoI TCCNGGA 1 cut(s) 1195
PkrI GCNGC 4 cut(s) 883, 886, 889, 1036
PleI GAGTC 2 cut(s) 494, 1315
PpsI GAGTC 2 cut(s) 494, 1315
Psp124BI GAGCTC 1 cut(s) 1154
Psp6I CCWGG 7 cut(s) 430, 723, 820, 904, 934, 1195, 1302
PspFI CCCAGC 1 cut(s) 305
PspGI CCWGG 7 cut(s) 430, 723, 820, 904, 934, 1195, 1302
PspN4I GGNNCC 2 cut(s) 517, 1312
PspPI GGNCC 4 cut(s) 6, 458, 1051, 1271
PstI CTGCAG 1 cut(s) 364
PstNI CAGNNNCTG 1 cut(s) 455
PsuI RGATCY 2 cut(s) 260, 515
RsaI GTAC 4 cut(s) 60, 283, 1089, 1249
RsaNI GTAC 4 cut(s) 59, 282, 1088, 1248
SacI GAGCTC 1 cut(s) 1154
SaqAI TTAA 6 cut(s) 234, 665, 702, 794, 1277, 1352
SatI GCNGC 4 cut(s) 882, 885, 888, 1035
Sau3AI GATC 3 cut(s) 260, 515, 1215
Sau96I GGNCC 4 cut(s) 6, 458, 1051, 1271
ScaI AGTACT 1 cut(s) 283
SchI GAGTC 2 cut(s) 494, 1315
ScrFI CCNGG 9 cut(s) 226, 227, 432, 725, 822, 906, 936, 1197, 1304
SduI GDGCHC 2 cut(s) 625, 1154
SfaNI GCATC 3 cut(s) 574, 696, 1214
SfcI CTRYAG 2 cut(s) 360, 831
SinI GGWCC 3 cut(s) 458, 1051, 1271
SmaI CCCGGG 1 cut(s) 227
SpeI ACTAGT 1 cut(s) 1333
SphI GCATGC 1 cut(s) 599
Sse9I AATT 9 cut(s) 202, 255, 342, 690, 699, 739, 752, 795, 1179
SsiI CCGC 2 cut(s) 634, 843
SspMI CTAG 2 cut(s) 1016, 1334
SstI GAGCTC 1 cut(s) 1154
StyD4I CCNGG 9 cut(s) 224, 225, 430, 723, 820, 904, 934, 1195, 1302
StyI CCWWGG 4 cut(s) 63, 474, 910, 961
TaaI ACNGT 3 cut(s) 378, 545, 1087
TasI AATT 9 cut(s) 202, 255, 342, 690, 699, 739, 752, 795, 1179
TatI WGTACW 3 cut(s) 281, 1087, 1247
TfiI GAWTC 3 cut(s) 170, 356, 712
Tru1I TTAA 6 cut(s) 234, 665, 702, 794, 1277, 1352
Tru9I TTAA 6 cut(s) 234, 665, 702, 794, 1277, 1352
TscAI CASTG 2 cut(s) 171, 1331
TseFI GTSAC 2 cut(s) 293, 938
TseI GCWGC 4 cut(s) 881, 884, 887, 1034
Tsp45I GTSAC 2 cut(s) 293, 938
TspDTI ATGAA 4 cut(s) 315, 639, 1155, 1251
TspGWI ACGGA 1 cut(s) 85
TspMI CCCGGG 1 cut(s) 225
TspRI CASTG 2 cut(s) 171, 1331
Van91I CCANNNNNTGG 2 cut(s) 845, 1184
VneI GTGCAC 1 cut(s) 621
VpaK11BI GGWCC 3 cut(s) 458, 1051, 1271
XapI RAATTY 1 cut(s) 752
XceI RCATGY 1 cut(s) 599
XmaI CCCGGG 1 cut(s) 225
XspI CTAG 2 cut(s) 1016, 1334
ZrmI AGTACT 1 cut(s) 283
Zsp2I ATGCAT 1 cut(s) 1207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.