Rw5G022220

Methionine gamma-lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0015573
Physical Location & Seq
Forward (+)
30514723 .. 30518979
4257 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G022220.1

Sequence Viewer

Length: 1311 bp
ATGTGGGTCTTATCCTGCGCCACCTCTGCTTATCCTGCGCTCACTTTCAGAGCTTCTGTACCCATGGCCACTACTCGTTCCGGCTCTCCCGTCTCCGTCTCAGTACCTACCAATCACCATTATTATCAGAAAGACAGCCCTTTTGTTCCAGAGGTCACATGCAACACCACCCGGGTTCTTAAAGCTTTCCAGAATGCCCGAATTGGATCTCATCACTTTGCTGGAAGTACTGGCTATGGTCATGATGAAGCTGGGGGACGTGAAGCTCTTGACCAAGCTTTTGCAGAAATTGTAGGCGCTGAATCTGCAGTAGTTCGCTCACAGTTTTTCTCAGGAACTCATGCTATAACCTGCGCTCTGTTTGCTTTTCTCAGGCCAGGAGATGAGCTTTTGGCAGTTGCTGGTCCTCCCTATGACACCTTGGAGGAGGTTATTGGAAAGAGAGACTCTCATGGCATGGGATCCTTGATAGACTTTGGAGTGAAATACCGGGAAGTTCCACTTGCTGAAGATGGTGGCCTTGACTGGGATGCGCTTAGGCATGCTTTGAACCCTGAAACAAAATGTGCACTCATACAGCGTTCATGTGGTTATTCATGGCGTCAGAGTTTAAGTGTGGATGATATAAGGCGAGCAATTGAGATAATTAAGATGCAGAATCCTAACTGCCTGGTCATGGTGGATAATTGTTATGGTGAATTTGTGGAAAGCATCGAACCTCCAATGGTGGGTGCAGATTTAATTGCTGGCAGTTTGATAAAAAATCCTGGTGGAACTATAGCCCCATGCGGTGGATATGTTGCAGGAAGAGAAAGATGGGTAAAAGCAGCAGCAGCTCGTCTGTCTGCACCTGGCCTTGGAGTTGATTCTGGCTCAACTCCTGGTCACATTATGAGAGCATTTTTCCAAGGTTTGTTCCTTTCACCTCAAATGGTTGGCGAGGCTATCAAGGGGACTTTCCTAGTAGCTGAAGTTATGGCTTCCAAAGGGTATAAGGTCCAGCCCCTTCCTCGTGTTCCTCGCCATGACACAGTACAGGCCATACAGCTTGGAAATCGTGAGCGTCTTCTTGCTTTCTGTGAGGCTGTGCAGAGGAGCTCCCCAGTCGGTTCATTTACTAAACCAATTGCTGGCACAACTCCTGGATATGCATCAGAGGTGATCTTTGCTGATGGAACATTTGTTGATGGGAGTACAAGTGAGCTTTCATGCGATGGACCATTAAGAGAGCCATTCGCTGTATTTTGCCAGGGTGGCTCCCATTGGACTCAGTGGGGACTAGTTCTTGGAGAGGTTTTAAAAGCCATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003962 GO:0004123 GO:0016740 GO:0016765 GO:0016829 GO:0016846
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Met_gamma_lyase

Protein Analysis

436

Amino Acids

46.73

Weight (kDa)

6.02

Isoelectric Point (pI)

39.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Met_gamma_lyase PF06838 52 - 434 3.2e-161 Methionine gamma-lyase
Cys_Met_Meta_PP PF01053 77 - 279 6.2e-09 Cys/Met metabolism PLP-dependent enzyme
Aminotran_1_2 PF00155 84 - 290 7.7e-06 Aminotransferase class I and II
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015573)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g20850 FvH4_3g20850 FvH4_3g20850
malus_domestica MD03G1220100.v1.1
prunus_persica Prupe.4G188800_v2.0.a1 Prupe.4G188800_v2.0.a1
pyrus_communis pycom03g16940
rosa_chinensis RchiOBHm_Chr5g0035161
rosa_laevigata RLG00000033603
rosa_multiflora Rmu_sc0000879.1_g000001 Rmu_sc0008176.1_g000007
rosa_roxburghii Rroxscaffold_1G00045250
rosa_rugosa Rorug05G0150400
rosa_samantha Rh5BG242800 Rh5DG251600
rosa_wichuraiana Rw5G022220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 359
AccB7I CCANNNNNTGG 2 cut(s) 791, 1130
AciI CCGC 1 cut(s) 789
AclWI GGATC 3 cut(s) 214, 456, 469
AcoI YGGCCR 1 cut(s) 66
AcsI RAATTY 1 cut(s) 698
AcuI CTGAAG 2 cut(s) 528, 990
AcyI GRCGYC 1 cut(s) 601
AfaI GTAC 5 cut(s) 60, 105, 229, 1035, 1195
AfiI CCNNNNNNNGG 6 cut(s) 526, 676, 728, 791, 857, 1130
AgsI TTSAA 1 cut(s) 550
AhlI ACTAGT 1 cut(s) 1279
AjiI CACGTC 1 cut(s) 260
AjnI CCWGG 7 cut(s) 376, 669, 766, 850, 880, 1141, 1248
Alw21I GWGCWC 2 cut(s) 571, 1100
Alw26I GTCTC 3 cut(s) 97, 103, 438
Alw44I GTGCAC 1 cut(s) 567
AlwI GGATC 3 cut(s) 214, 456, 469
AlwNI CAGNNNCTG 1 cut(s) 401
Ama87I CYCGRG 1 cut(s) 171
AoxI GGCC 5 cut(s) 66, 374, 517, 853, 1038
ApaLI GTGCAC 1 cut(s) 567
ApeKI GCWGC 3 cut(s) 827, 830, 833
ApoI RAATTY 1 cut(s) 698
ArsI GACNNNNNNTTYG 4 cut(s) 125, 157, 263, 295
AspLEI GCGC 5 cut(s) 20, 40, 299, 356, 535
AspS9I GGNCC 3 cut(s) 404, 997, 1217
AsuC2I CCSGG 3 cut(s) 172, 173, 491
AsuHPI GGTGA 4 cut(s) 107, 707, 915, 1171
AvaI CYCGRG 1 cut(s) 171
AvaII GGWCC 3 cut(s) 404, 997, 1217
BaeGI GKGCMC 1 cut(s) 571
BalI TGGCCA 1 cut(s) 68
BamHI GGATCC 1 cut(s) 461
BanII GRGCYC 1 cut(s) 1100
BauI CACGAG 1 cut(s) 1011
BbsI GAAGAC 1 cut(s) 1058
Bbv12I GWGCWC 2 cut(s) 571, 1100
BbvI GCAGC 3 cut(s) 839, 842, 845
BccI CCATC 5 cut(s) 506, 810, 1166, 1181, 1208
BciT130I CCWGG 7 cut(s) 378, 671, 768, 852, 882, 1143, 1250
BcnI CCSGG 3 cut(s) 172, 173, 491
BcoDI GTCTC 3 cut(s) 97, 103, 438
BcuI ACTAGT 1 cut(s) 1279
BfaI CTAG 2 cut(s) 962, 1280
BfmI CTRYAG 2 cut(s) 306, 777
BfoI RGCGCY 1 cut(s) 300
BfuAI ACCTGC 1 cut(s) 359
BglI GCCNNNNNGGC 1 cut(s) 1254
BisI GCNGC 3 cut(s) 828, 831, 834
BlsI GCNGC 3 cut(s) 829, 832, 835
BmcAI AGTACT 1 cut(s) 229
Bme18I GGWCC 3 cut(s) 404, 997, 1217
BmeT110I CYCGRG 1 cut(s) 171
BmgBI CACGTC 1 cut(s) 260
BmgT120I GGNCC 3 cut(s) 404, 997, 1217
BmiI GGNNCC 2 cut(s) 463, 1258
BmrI ACTGGG 2 cut(s) 535, 1097
BmsI GCATC 4 cut(s) 520, 642, 720, 1160
BmuI ACTGGG 2 cut(s) 535, 1097
BpiI GAAGAC 1 cut(s) 1058
BplI GAGNNNNNCTC 2 cut(s) 433, 465
Bpu10I CCTNAGC 1 cut(s) 536
BpuMI CCSGG 3 cut(s) 172, 173, 491
BsaBI GATNNNNATC 1 cut(s) 1150
BsaHI GRCGYC 1 cut(s) 601
BsaJI CCNNGG 6 cut(s) 63, 171, 420, 856, 907, 1249
BsaXI ACNNNNNCTCC 2 cut(s) 1183, 1213
Bsc4I CCNNNNNNNGG 6 cut(s) 526, 676, 728, 791, 857, 1130
Bse1I ACTGG 3 cut(s) 235, 530, 1103
Bse8I GATNNNNATC 1 cut(s) 1150
BseBI CCWGG 7 cut(s) 378, 671, 768, 852, 882, 1143, 1250
BseDI CCNNGG 6 cut(s) 63, 171, 420, 856, 907, 1249
BseGI GGATG 2 cut(s) 535, 625
BseJI GATNNNNATC 1 cut(s) 1150
BseLI CCNNNNNNNGG 6 cut(s) 526, 676, 728, 791, 857, 1130
BseMII CTCAG 4 cut(s) 114, 345, 385, 1283
BseNI ACTGG 3 cut(s) 235, 530, 1103
BseRI GAGGAG 2 cut(s) 440, 1108
BseSI GKGCMC 1 cut(s) 571
BseXI GCAGC 3 cut(s) 839, 842, 845
BseYI CCCAGC 1 cut(s) 251
BsgI GTGCAG 3 cut(s) 753, 831, 1109
BshFI GGCC 5 cut(s) 68, 376, 519, 855, 1040
BsiHKAI GWGCWC 2 cut(s) 571, 1100
BsiHKCI CYCGRG 1 cut(s) 171
BsiSI CCGG 3 cut(s) 81, 172, 490
BslFI GGGAC 3 cut(s) 270, 967, 1290
BslI CCNNNNNNNGG 6 cut(s) 526, 676, 728, 791, 857, 1130
BsmAI GTCTC 3 cut(s) 97, 103, 438
BsmBI CGTCTC 2 cut(s) 97, 103
BsmFI GGGAC 3 cut(s) 270, 967, 1290
BsmI GAATGC 1 cut(s) 199
BsnI GGCC 5 cut(s) 68, 376, 519, 855, 1040
BsoBI CYCGRG 1 cut(s) 171
Bsp1286I GDGCHC 2 cut(s) 571, 1100
Bsp143I GATC 3 cut(s) 206, 461, 1161
Bsp19I CCATGG 1 cut(s) 63
BspACI CCGC 1 cut(s) 789
BspANI GGCC 5 cut(s) 68, 376, 519, 855, 1040
BspCNI CTCAG 4 cut(s) 113, 344, 384, 1282
BspHI TCATGA 1 cut(s) 241
BspLI GGNNCC 2 cut(s) 463, 1258
BspMAI CTGCAG 1 cut(s) 310
BspMI ACCTGC 1 cut(s) 359
BspPI GGATC 3 cut(s) 214, 456, 469
BsrI ACTGG 3 cut(s) 235, 530, 1103
BssECI CCNNGG 6 cut(s) 63, 171, 420, 856, 907, 1249
BssMI GATC 3 cut(s) 206, 461, 1161
BssNI GRCGYC 1 cut(s) 601
BssSI CACGAG 1 cut(s) 1011
BssT1I CCWWGG 4 cut(s) 63, 420, 856, 907
Bst2BI CACGAG 1 cut(s) 1011
Bst2UI CCWGG 7 cut(s) 378, 671, 768, 852, 882, 1143, 1250
Bst4CI ACNGT 2 cut(s) 324, 1033
Bst6I CTCTTC 1 cut(s) 802
BstACI GRCGYC 1 cut(s) 601
BstC8I GCNNGC 4 cut(s) 543, 633, 748, 1132
BstDEI CTNAG 5 cut(s) 100, 331, 371, 536, 1269
BstDSI CCRYGG 1 cut(s) 63
BstF5I GGATG 2 cut(s) 535, 625
BstH2I RGCGCY 1 cut(s) 300
BstHHI GCGC 5 cut(s) 20, 40, 299, 356, 535
BstKTI GATC 3 cut(s) 209, 464, 1164
BstMAI GTCTC 3 cut(s) 97, 103, 438
BstMBI GATC 3 cut(s) 206, 461, 1161
BstMWI GCNNNNNNNGC 6 cut(s) 26, 35, 305, 362, 833, 1254
BstNI CCWGG 7 cut(s) 378, 671, 768, 852, 882, 1143, 1250
BstNSI RCATGY 2 cut(s) 162, 545
BstSFI CTRYAG 2 cut(s) 306, 777
BstSLI GKGCMC 1 cut(s) 571
BstV1I GCAGC 3 cut(s) 839, 842, 845
BstV2I GAAGAC 1 cut(s) 1058
BstX2I RGATCY 2 cut(s) 206, 461
BstYI RGATCY 2 cut(s) 206, 461
BsuRI GGCC 5 cut(s) 68, 376, 519, 855, 1040
BtgI CCRYGG 1 cut(s) 63
BtgZI GCGATG 1 cut(s) 1227
BtrI CACGTC 1 cut(s) 260
BtsCI GGATG 2 cut(s) 535, 625
BtsIMutI CAGTG 1 cut(s) 1277
BveI ACCTGC 1 cut(s) 359
Cac8I GCNNGC 4 cut(s) 543, 633, 748, 1132
CaiI CAGNNNCTG 1 cut(s) 401
CciI TCATGA 1 cut(s) 241
CfoI GCGC 5 cut(s) 20, 40, 299, 356, 535
Cfr13I GGNCC 3 cut(s) 404, 997, 1217
Cfr9I CCCGGG 1 cut(s) 171
CseI GACGC 2 cut(s) 590, 1052
Csp6I GTAC 5 cut(s) 59, 104, 228, 1034, 1194
CviQI GTAC 5 cut(s) 59, 104, 228, 1034, 1194
DdeI CTNAG 5 cut(s) 100, 331, 371, 536, 1269
DpnI GATC 3 cut(s) 208, 463, 1163
DpnII GATC 3 cut(s) 206, 461, 1161
DraI TTTAAA 1 cut(s) 1299
EaeI YGGCCR 1 cut(s) 66
Eam1104I CTCTTC 1 cut(s) 802
EarI CTCTTC 1 cut(s) 802
Ecl136II GAGCTC 1 cut(s) 1098
Eco130I CCWWGG 4 cut(s) 63, 420, 856, 907
Eco24I GRGCYC 1 cut(s) 1100
Eco47I GGWCC 3 cut(s) 404, 997, 1217
Eco53kI GAGCTC 1 cut(s) 1098
Eco57I CTGAAG 2 cut(s) 528, 990
Eco88I CYCGRG 1 cut(s) 171
EcoICRI GAGCTC 1 cut(s) 1098
EcoRII CCWGG 7 cut(s) 376, 669, 766, 850, 880, 1141, 1248
EcoT14I CCWWGG 4 cut(s) 63, 420, 856, 907
EcoT22I ATGCAT 1 cut(s) 1153
EcoT38I GRGCYC 1 cut(s) 1100
ErhI CCWWGG 4 cut(s) 63, 420, 856, 907
Esp3I CGTCTC 2 cut(s) 97, 103
FalI AAGNNNNNCTT 2 cut(s) 486, 518
FaqI GGGAC 3 cut(s) 270, 967, 1290
Fnu4HI GCNGC 3 cut(s) 828, 831, 834
FokI GGATG 2 cut(s) 542, 632
FriOI GRGCYC 1 cut(s) 1100
Fsp4HI GCNGC 3 cut(s) 828, 831, 834
FspBI CTAG 2 cut(s) 962, 1280
GlaI GCGC 5 cut(s) 19, 39, 298, 355, 534
GluI GCNGC 3 cut(s) 828, 831, 834
GsaI CCCAGC 1 cut(s) 255
HaeII RGCGCY 1 cut(s) 300
HaeIII GGCC 5 cut(s) 68, 376, 519, 855, 1040
HapII CCGG 3 cut(s) 81, 172, 490
HgaI GACGC 2 cut(s) 590, 1052
HhaI GCGC 5 cut(s) 20, 40, 299, 356, 535
Hin1I GRCGYC 1 cut(s) 601
Hin6I GCGC 5 cut(s) 18, 38, 297, 354, 533
HinP1I GCGC 5 cut(s) 18, 38, 297, 354, 533
HindIII AAGCTT 2 cut(s) 183, 276
HinfI GANTC 5 cut(s) 302, 446, 658, 866, 1267
HpaII CCGG 3 cut(s) 81, 172, 490
HphI GGTGA 4 cut(s) 107, 707, 915, 1171
Hpy166II GTNNAC 1 cut(s) 569
Hpy188I TCNGA 4 cut(s) 50, 129, 606, 1156
Hpy188III TCNNGA 6 cut(s) 149, 190, 242, 269, 333, 1058
Hpy8I GTNNAC 1 cut(s) 569
HpyAV CCTTC 1 cut(s) 1016
HpyCH4III ACNGT 2 cut(s) 324, 1033
HpyCH4IV ACGT 1 cut(s) 259
HpyF10VI GCNNNNNNNGC 6 cut(s) 26, 35, 305, 362, 833, 1254
HpyF3I CTNAG 5 cut(s) 100, 331, 371, 536, 1269
HpySE526I ACGT 1 cut(s) 259
Hsp92I GRCGYC 1 cut(s) 601
HspAI GCGC 5 cut(s) 18, 38, 297, 354, 533
Kzo9I GATC 3 cut(s) 206, 461, 1161
LmnI GCTCC 3 cut(s) 1095, 1103, 1262
Lsp1109I GCAGC 3 cut(s) 839, 842, 845
LweI GCATC 4 cut(s) 520, 642, 720, 1160
MaeI CTAG 2 cut(s) 962, 1280
MaeII ACGT 1 cut(s) 259
MaeIII GTNAC 2 cut(s) 154, 884
MalI GATC 3 cut(s) 208, 463, 1163
MboI GATC 3 cut(s) 206, 461, 1161
MboII GAAGA 3 cut(s) 521, 819, 1058
MfeI CAATTG 2 cut(s) 636, 1125
MflI RGATCY 2 cut(s) 206, 461
MhlI GDGCHC 2 cut(s) 571, 1100
MlsI TGGCCA 1 cut(s) 68
MluCI AATT 8 cut(s) 201, 288, 636, 645, 685, 698, 741, 1125
MluNI TGGCCA 1 cut(s) 68
MlyI GAGTC 2 cut(s) 440, 1261
Mox20I TGGCCA 1 cut(s) 68
Mph1103I ATGCAT 1 cut(s) 1153
MscI TGGCCA 1 cut(s) 68
MseI TTAA 6 cut(s) 180, 611, 648, 740, 1223, 1298
Msp20I TGGCCA 1 cut(s) 68
MspI CCGG 3 cut(s) 81, 172, 490
MunI CAATTG 2 cut(s) 636, 1125
Mva1269I GAATGC 1 cut(s) 199
MvaI CCWGG 7 cut(s) 378, 671, 768, 852, 882, 1143, 1250
MwoI GCNNNNNNNGC 6 cut(s) 26, 35, 305, 362, 833, 1254
NciI CCSGG 3 cut(s) 172, 173, 491
NcoI CCATGG 1 cut(s) 63
NdeII GATC 3 cut(s) 206, 461, 1161
NlaIV GGNNCC 2 cut(s) 463, 1258
NmuCI GTSAC 2 cut(s) 154, 884
NsiI ATGCAT 1 cut(s) 1153
NspI RCATGY 2 cut(s) 162, 545
PaeI GCATGC 1 cut(s) 545
PagI TCATGA 1 cut(s) 241
PctI GAATGC 1 cut(s) 199
PfeI GAWTC 3 cut(s) 302, 658, 866
PflMI CCANNNNNTGG 2 cut(s) 791, 1130
PfoI TCCNGGA 1 cut(s) 1141
PkrI GCNGC 3 cut(s) 829, 832, 835
PleI GAGTC 2 cut(s) 440, 1261
PpsI GAGTC 2 cut(s) 440, 1261
Psp124BI GAGCTC 1 cut(s) 1100
Psp6I CCWGG 7 cut(s) 376, 669, 766, 850, 880, 1141, 1248
PspFI CCCAGC 1 cut(s) 251
PspGI CCWGG 7 cut(s) 376, 669, 766, 850, 880, 1141, 1248
PspN4I GGNNCC 2 cut(s) 463, 1258
PspPI GGNCC 3 cut(s) 404, 997, 1217
PstI CTGCAG 1 cut(s) 310
PstNI CAGNNNCTG 1 cut(s) 401
PsuI RGATCY 2 cut(s) 206, 461
RsaI GTAC 5 cut(s) 60, 105, 229, 1035, 1195
RsaNI GTAC 5 cut(s) 59, 104, 228, 1034, 1194
SacI GAGCTC 1 cut(s) 1100
SaqAI TTAA 6 cut(s) 180, 611, 648, 740, 1223, 1298
SatI GCNGC 3 cut(s) 828, 831, 834
Sau3AI GATC 3 cut(s) 206, 461, 1161
Sau96I GGNCC 3 cut(s) 404, 997, 1217
ScaI AGTACT 1 cut(s) 229
SchI GAGTC 2 cut(s) 440, 1261
SduI GDGCHC 2 cut(s) 571, 1100
SfaNI GCATC 4 cut(s) 520, 642, 720, 1160
SfcI CTRYAG 2 cut(s) 306, 777
SinI GGWCC 3 cut(s) 404, 997, 1217
SmaI CCCGGG 1 cut(s) 173
SpeI ACTAGT 1 cut(s) 1279
SphI GCATGC 1 cut(s) 545
Sse9I AATT 8 cut(s) 201, 288, 636, 645, 685, 698, 741, 1125
SsiI CCGC 1 cut(s) 789
SspMI CTAG 2 cut(s) 962, 1280
SstI GAGCTC 1 cut(s) 1100
StyI CCWWGG 4 cut(s) 63, 420, 856, 907
TaaI ACNGT 2 cut(s) 324, 1033
TaiI ACGT 1 cut(s) 262
TaqI TCGA 1 cut(s) 714
TasI AATT 8 cut(s) 201, 288, 636, 645, 685, 698, 741, 1125
TatI WGTACW 3 cut(s) 227, 1033, 1193
TfiI GAWTC 3 cut(s) 302, 658, 866
Tru1I TTAA 6 cut(s) 180, 611, 648, 740, 1223, 1298
Tru9I TTAA 6 cut(s) 180, 611, 648, 740, 1223, 1298
TscAI CASTG 1 cut(s) 1277
TseFI GTSAC 2 cut(s) 154, 884
TseI GCWGC 3 cut(s) 827, 830, 833
Tsp45I GTSAC 2 cut(s) 154, 884
TspDTI ATGAA 5 cut(s) 261, 573, 585, 1101, 1197
TspGWI ACGGA 1 cut(s) 85
TspMI CCCGGG 1 cut(s) 171
TspRI CASTG 1 cut(s) 1277
Van91I CCANNNNNTGG 2 cut(s) 791, 1130
VneI GTGCAC 1 cut(s) 567
VpaK11BI GGWCC 3 cut(s) 404, 997, 1217
XapI RAATTY 1 cut(s) 698
XceI RCATGY 2 cut(s) 162, 545
XmaI CCCGGG 1 cut(s) 171
XspI CTAG 2 cut(s) 962, 1280
ZrmI AGTACT 1 cut(s) 229
Zsp2I ATGCAT 1 cut(s) 1153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.