Rroxscaffold_1G00045250

Methionine gamma-lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0015573
Physical Location & Seq
Reverse (-)
64258009 .. 64263284
5276 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00045250.1

Sequence Viewer

Length: 1494 bp
ATGTGGGCCTTATCCTGCGCCACCTCTGCTTATCCTGCGCTCACTTTCAGAGCTTCTGTACCCATGGCCACTACTCGTTCCGGTTCTCCCGTCTCCGTCTCAGTCCCTACCAATCACCATTGTTATCAGAAAGACAGTCCTTTTGTTCCAGAGGTTGTGGAAGCAGTGGATGCCCTGCATTCAGAGTTCAGAGCTGTGGATAATTTGGTAGCACGCAATACCACCCGGGTTCTTAAAGCTTTCCAGAATGCCCGAATTGGATCTCATCACTTTGCTGGAAGTACTGGCTATGGTCATGATGAAGCTGGGGGACGTGAAGCTCTTGACCAAGCTTTTGCAGAAATTGTAGGTGCTGAATCTGCAATAGTTCGCTCACAGAGGTACTTGCAATACTTTGTTAATCCTTTTCTCCAATGCCTTCAGTTTTTCTCAGGAACTCATGCTATAACCTGTGCTCTGTTTGCTTTTCTAAGGCCAGGAGATGAGCTTTTGGCAGTTGCTGGTCCTCCCTATGATACCTTGGAGGAAGTTATTGGAAAGAGAGACTCTCATGGCATGGGATCCTTGATAGACTTTGGAGTGAAATACCGTGAAGTTCCGCTTGCTGAAGATGGTGGCCTTGACTGGGATGCGCTTAGGCATGCTTTGAACCCTGAAACAAAATGTGCACTCATTCAGCGGTCATGTGGTTATTCATGGCGTCAGAGTTTAAGTGTGGATGATATAAGGCGAGCAATTGAGATAATTAAGATGCAGAATCCTAACTGCCTGGTCATGGTGGATAACTGTTATGGTGAATTTGTGGAAAGCATTGAACCTCCGATGGTGGTGCGTGACATATTTTGTATCGACTTTAGATCTCTTATTTTGAAAGAAAAAGATAAAGGCAATAAAATGCTGTTATGTGTTCAGGGTGCAGATTTAATTGCTGGCAGTTTGATAAAAAATCCTGGTGGAACTATAGCCCCATGTGGTGGATATGTTGCAGGAAGAGAAAGATGGGTAAAAGCAGCAGCAGCTCGTCTGTCTGCACCTGGCCTTGGAGTTGATTGTGGCTCAACTCCTGGTCACACTATGAGAGCATTTTTCCAAGGTTTGTTCCTTTCACCTCAAATGGTTGGCGAGGCTATCAAGGGGACTTTCCTAGTAGCTGAAGTTATGGCTTCCAAAGGGTATAAAGTCCAGCCCCTTCCTCGTGTTCCTCGCCATGACACAGTACAGGCCATACAGCTTGGAAATCGTGAGCATCTTCTTGCTTTCTGTGAGGCTGTGCAGAGGAGCTCCCCAGTCGGTTCATTTACTAAACCAATTGCTGGCACAACTCCTGGATATGCATCAGAGGTGATATTTGCTGATGGAACATTTGTTGATGGGAGTACAAGTGAGCTTTCATGCGATGGACCATTAAGAGAGCCATTCGCTGTATTTTGCCAGGGTGGCTCCCATTGGACTCAGTGGGGACTAGTTCTTGGAGAGGTTTTAAAAGCCATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003962 GO:0004123 GO:0016740 GO:0016765 GO:0016829 GO:0016846
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Met_gamma_lyase

Protein Analysis

497

Amino Acids

53.86

Weight (kDa)

6.13

Isoelectric Point (pI)

36.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Met_gamma_lyase PF06838 54 - 281 6.1e-76 Methionine gamma-lyase
Met_gamma_lyase PF06838 305 - 495 2.9e-74 Methionine gamma-lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015573)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g20850 FvH4_3g20850 FvH4_3g20850
malus_domestica MD03G1220100.v1.1
prunus_persica Prupe.4G188800_v2.0.a1 Prupe.4G188800_v2.0.a1
pyrus_communis pycom03g16940
rosa_chinensis RchiOBHm_Chr5g0035161
rosa_laevigata RLG00000033603
rosa_multiflora Rmu_sc0000879.1_g000001 Rmu_sc0008176.1_g000007
rosa_roxburghii Rroxscaffold_1G00045250
rosa_rugosa Rorug05G0150400
rosa_samantha Rh5BG242800 Rh5DG251600
rosa_wichuraiana Rw5G022220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 974, 1313
AciI CCGC 2 cut(s) 599, 679
AclWI GGATC 3 cut(s) 268, 555, 568
AcoI YGGCCR 1 cut(s) 66
AcsI RAATTY 1 cut(s) 797
AcuI CTGAAG 3 cut(s) 404, 627, 1173
AcyI GRCGYC 1 cut(s) 700
AfaI GTAC 5 cut(s) 60, 283, 383, 1218, 1378
AfiI CCNNNNNNNGG 5 cut(s) 625, 775, 974, 1040, 1313
AgsI TTSAA 3 cut(s) 649, 815, 871
AhlI ACTAGT 1 cut(s) 1462
AjiI CACGTC 1 cut(s) 314
AjnI CCWGG 7 cut(s) 475, 768, 949, 1033, 1063, 1324, 1431
Alw21I GWGCWC 3 cut(s) 457, 670, 1283
Alw26I GTCTC 3 cut(s) 97, 103, 537
Alw44I GTGCAC 1 cut(s) 666
AlwI GGATC 3 cut(s) 268, 555, 568
AlwNI CAGNNNCTG 1 cut(s) 500
Ama87I CYCGRG 1 cut(s) 225
AoxI GGCC 6 cut(s) 6, 66, 473, 616, 1036, 1221
ApaLI GTGCAC 1 cut(s) 666
ApeKI GCWGC 3 cut(s) 1010, 1013, 1016
ApoI RAATTY 1 cut(s) 797
ArsI GACNNNNNNTTYG 4 cut(s) 125, 157, 317, 349
AspLEI GCGC 3 cut(s) 20, 40, 634
AspS9I GGNCC 3 cut(s) 6, 503, 1400
AsuC2I CCSGG 2 cut(s) 226, 227
AsuHPI GGTGA 4 cut(s) 107, 806, 1098, 1354
AvaI CYCGRG 1 cut(s) 225
AvaII GGWCC 2 cut(s) 503, 1400
BaeGI GKGCMC 1 cut(s) 670
BalI TGGCCA 1 cut(s) 68
BamHI GGATCC 1 cut(s) 560
BanII GRGCYC 1 cut(s) 1283
BauI CACGAG 1 cut(s) 1194
Bbv12I GWGCWC 3 cut(s) 457, 670, 1283
BbvI GCAGC 3 cut(s) 1022, 1025, 1028
BccI CCATC 6 cut(s) 605, 817, 993, 1349, 1364, 1391
BcgI CGANNNNNNTGC 2 cut(s) 811, 845
BciT130I CCWGG 7 cut(s) 477, 770, 951, 1035, 1065, 1326, 1433
BcnI CCSGG 2 cut(s) 226, 227
BcoDI GTCTC 3 cut(s) 97, 103, 537
BcuI ACTAGT 1 cut(s) 1462
BfaI CTAG 2 cut(s) 1145, 1463
BfmI CTRYAG 1 cut(s) 960
BglI GCCNNNNNGGC 1 cut(s) 1437
BglII AGATCT 1 cut(s) 857
BisI GCNGC 3 cut(s) 1011, 1014, 1017
BlsI GCNGC 3 cut(s) 1012, 1015, 1018
BmcAI AGTACT 1 cut(s) 283
Bme1390I CCNGG 9 cut(s) 226, 227, 477, 770, 951, 1035, 1065, 1326, 1433
Bme18I GGWCC 2 cut(s) 503, 1400
BmeT110I CYCGRG 1 cut(s) 225
BmgBI CACGTC 1 cut(s) 314
BmgT120I GGNCC 3 cut(s) 6, 503, 1400
BmiI GGNNCC 2 cut(s) 562, 1441
BmrFI CCNGG 9 cut(s) 226, 227, 477, 770, 951, 1035, 1065, 1326, 1433
BmrI ACTGGG 2 cut(s) 634, 1280
BmsI GCATC 5 cut(s) 160, 619, 741, 1255, 1343
BmuI ACTGGG 2 cut(s) 634, 1280
BplI GAGNNNNNCTC 2 cut(s) 532, 564
Bpu10I CCTNAGC 1 cut(s) 635
BpuMI CCSGG 2 cut(s) 226, 227
BsaBI GATNNNNATC 1 cut(s) 1333
BsaHI GRCGYC 1 cut(s) 700
BsaJI CCNNGG 6 cut(s) 63, 225, 519, 1039, 1090, 1432
BsaWI WCCGGW 1 cut(s) 80
BsaXI ACNNNNNCTCC 2 cut(s) 1366, 1396
Bsc4I CCNNNNNNNGG 5 cut(s) 625, 775, 974, 1040, 1313
Bse1I ACTGG 3 cut(s) 289, 629, 1286
Bse8I GATNNNNATC 1 cut(s) 1333
BseBI CCWGG 7 cut(s) 477, 770, 951, 1035, 1065, 1326, 1433
BseDI CCNNGG 6 cut(s) 63, 225, 519, 1039, 1090, 1432
BseGI GGATG 3 cut(s) 175, 634, 724
BseJI GATNNNNATC 1 cut(s) 1333
BseLI CCNNNNNNNGG 5 cut(s) 625, 775, 974, 1040, 1313
BseMII CTCAG 3 cut(s) 114, 444, 1466
BseNI ACTGG 3 cut(s) 289, 629, 1286
BseRI GAGGAG 1 cut(s) 1291
BseSI GKGCMC 1 cut(s) 670
BseXI GCAGC 3 cut(s) 1022, 1025, 1028
BseYI CCCAGC 1 cut(s) 305
BsgI GTGCAG 3 cut(s) 936, 1014, 1292
BshFI GGCC 6 cut(s) 8, 68, 475, 618, 1038, 1223
BsiHKAI GWGCWC 3 cut(s) 457, 670, 1283
BsiHKCI CYCGRG 1 cut(s) 225
BsiSI CCGG 2 cut(s) 81, 226
BslFI GGGAC 4 cut(s) 89, 324, 1150, 1473
BslI CCNNNNNNNGG 5 cut(s) 625, 775, 974, 1040, 1313
BsmAI GTCTC 3 cut(s) 97, 103, 537
BsmBI CGTCTC 2 cut(s) 97, 103
BsmFI GGGAC 4 cut(s) 89, 324, 1150, 1473
BsmI GAATGC 2 cut(s) 178, 253
BsnI GGCC 6 cut(s) 8, 68, 475, 618, 1038, 1223
BsoBI CYCGRG 1 cut(s) 225
Bsp1286I GDGCHC 3 cut(s) 457, 670, 1283
Bsp143I GATC 3 cut(s) 260, 560, 857
Bsp19I CCATGG 1 cut(s) 63
BspACI CCGC 2 cut(s) 599, 679
BspANI GGCC 6 cut(s) 8, 68, 475, 618, 1038, 1223
BspCNI CTCAG 3 cut(s) 113, 443, 1465
BspHI TCATGA 1 cut(s) 295
BspLI GGNNCC 2 cut(s) 562, 1441
BspPI GGATC 3 cut(s) 268, 555, 568
BsrI ACTGG 3 cut(s) 289, 629, 1286
BssECI CCNNGG 6 cut(s) 63, 225, 519, 1039, 1090, 1432
BssMI GATC 3 cut(s) 260, 560, 857
BssNI GRCGYC 1 cut(s) 700
BssSI CACGAG 1 cut(s) 1194
BssT1I CCWWGG 4 cut(s) 63, 519, 1039, 1090
Bst2BI CACGAG 1 cut(s) 1194
Bst2UI CCWGG 7 cut(s) 477, 770, 951, 1035, 1065, 1326, 1433
Bst4CI ACNGT 4 cut(s) 137, 590, 788, 1216
Bst6I CTCTTC 1 cut(s) 985
BstACI GRCGYC 1 cut(s) 700
BstAPI GCANNNNNTGC 1 cut(s) 170
BstC8I GCNNGC 6 cut(s) 214, 603, 642, 732, 931, 1315
BstDEI CTNAG 5 cut(s) 100, 430, 470, 635, 1452
BstDSI CCRYGG 1 cut(s) 63
BstF5I GGATG 3 cut(s) 175, 634, 724
BstHHI GCGC 3 cut(s) 20, 40, 634
BstKTI GATC 3 cut(s) 263, 563, 860
BstMAI GTCTC 3 cut(s) 97, 103, 537
BstMBI GATC 3 cut(s) 260, 560, 857
BstMWI GCNNNNNNNGC 7 cut(s) 26, 35, 170, 359, 461, 1016, 1437
BstNI CCWGG 7 cut(s) 477, 770, 951, 1035, 1065, 1326, 1433
BstNSI RCATGY 1 cut(s) 644
BstSCI CCNGG 9 cut(s) 224, 225, 475, 768, 949, 1033, 1063, 1324, 1431
BstSFI CTRYAG 1 cut(s) 960
BstSLI GKGCMC 1 cut(s) 670
BstV1I GCAGC 3 cut(s) 1022, 1025, 1028
BstX2I RGATCY 3 cut(s) 260, 560, 857
BstYI RGATCY 3 cut(s) 260, 560, 857
BsuRI GGCC 6 cut(s) 8, 68, 475, 618, 1038, 1223
BtgI CCRYGG 1 cut(s) 63
BtgZI GCGATG 1 cut(s) 1410
BtrI CACGTC 1 cut(s) 314
BtsCI GGATG 3 cut(s) 175, 634, 724
BtsI GCAGTG 1 cut(s) 171
BtsIMutI CAGTG 2 cut(s) 171, 1460
Cac8I GCNNGC 6 cut(s) 214, 603, 642, 732, 931, 1315
CaiI CAGNNNCTG 1 cut(s) 500
CciI TCATGA 1 cut(s) 295
CfoI GCGC 3 cut(s) 20, 40, 634
Cfr13I GGNCC 3 cut(s) 6, 503, 1400
Cfr9I CCCGGG 1 cut(s) 225
CseI GACGC 1 cut(s) 689
Csp6I GTAC 5 cut(s) 59, 282, 382, 1217, 1377
CviQI GTAC 5 cut(s) 59, 282, 382, 1217, 1377
DdeI CTNAG 5 cut(s) 100, 430, 470, 635, 1452
DpnI GATC 3 cut(s) 262, 562, 859
DpnII GATC 3 cut(s) 260, 560, 857
DraI TTTAAA 1 cut(s) 1482
EaeI YGGCCR 1 cut(s) 66
Eam1104I CTCTTC 1 cut(s) 985
EarI CTCTTC 1 cut(s) 985
Ecl136II GAGCTC 1 cut(s) 1281
Eco130I CCWWGG 4 cut(s) 63, 519, 1039, 1090
Eco24I GRGCYC 1 cut(s) 1283
Eco47I GGWCC 2 cut(s) 503, 1400
Eco53kI GAGCTC 1 cut(s) 1281
Eco57I CTGAAG 3 cut(s) 404, 627, 1173
Eco88I CYCGRG 1 cut(s) 225
EcoICRI GAGCTC 1 cut(s) 1281
EcoRII CCWGG 7 cut(s) 475, 768, 949, 1033, 1063, 1324, 1431
EcoT14I CCWWGG 4 cut(s) 63, 519, 1039, 1090
EcoT22I ATGCAT 1 cut(s) 1336
EcoT38I GRGCYC 1 cut(s) 1283
ErhI CCWWGG 4 cut(s) 63, 519, 1039, 1090
Esp3I CGTCTC 2 cut(s) 97, 103
FalI AAGNNNNNCTT 2 cut(s) 585, 617
FaqI GGGAC 4 cut(s) 89, 324, 1150, 1473
Fnu4HI GCNGC 3 cut(s) 1011, 1014, 1017
FokI GGATG 3 cut(s) 182, 641, 731
FriOI GRGCYC 1 cut(s) 1283
Fsp4HI GCNGC 3 cut(s) 1011, 1014, 1017
FspBI CTAG 2 cut(s) 1145, 1463
GlaI GCGC 3 cut(s) 19, 39, 633
GluI GCNGC 3 cut(s) 1011, 1014, 1017
GsaI CCCAGC 1 cut(s) 309
HaeIII GGCC 6 cut(s) 8, 68, 475, 618, 1038, 1223
HapII CCGG 2 cut(s) 81, 226
HgaI GACGC 1 cut(s) 689
HhaI GCGC 3 cut(s) 20, 40, 634
Hin1I GRCGYC 1 cut(s) 700
Hin6I GCGC 3 cut(s) 18, 38, 632
HinP1I GCGC 3 cut(s) 18, 38, 632
HindIII AAGCTT 2 cut(s) 237, 330
HinfI GANTC 4 cut(s) 356, 545, 757, 1450
HpaII CCGG 2 cut(s) 81, 226
HphI GGTGA 4 cut(s) 107, 806, 1098, 1354
Hpy166II GTNNAC 1 cut(s) 668
Hpy188I TCNGA 7 cut(s) 50, 129, 184, 191, 705, 822, 1339
Hpy188III TCNNGA 6 cut(s) 149, 244, 296, 323, 432, 1241
Hpy8I GTNNAC 1 cut(s) 668
HpyAV CCTTC 2 cut(s) 428, 1199
HpyCH4III ACNGT 4 cut(s) 137, 590, 788, 1216
HpyCH4IV ACGT 1 cut(s) 313
HpyF10VI GCNNNNNNNGC 7 cut(s) 26, 35, 170, 359, 461, 1016, 1437
HpyF3I CTNAG 5 cut(s) 100, 430, 470, 635, 1452
HpySE526I ACGT 1 cut(s) 313
Hsp92I GRCGYC 1 cut(s) 700
HspAI GCGC 3 cut(s) 18, 38, 632
Kzo9I GATC 3 cut(s) 260, 560, 857
LmnI GCTCC 3 cut(s) 1278, 1286, 1445
Lsp1109I GCAGC 3 cut(s) 1022, 1025, 1028
LweI GCATC 5 cut(s) 160, 619, 741, 1255, 1343
MaeI CTAG 2 cut(s) 1145, 1463
MaeII ACGT 1 cut(s) 313
MaeIII GTNAC 2 cut(s) 833, 1067
MalI GATC 3 cut(s) 262, 562, 859
MboI GATC 3 cut(s) 260, 560, 857
MboII GAAGA 3 cut(s) 620, 1002, 1241
MfeI CAATTG 2 cut(s) 735, 1308
MflI RGATCY 3 cut(s) 260, 560, 857
MhlI GDGCHC 3 cut(s) 457, 670, 1283
MlsI TGGCCA 1 cut(s) 68
MluCI AATT 8 cut(s) 202, 255, 342, 735, 744, 797, 924, 1308
MluNI TGGCCA 1 cut(s) 68
MlyI GAGTC 2 cut(s) 539, 1444
Mox20I TGGCCA 1 cut(s) 68
Mph1103I ATGCAT 1 cut(s) 1336
MscI TGGCCA 1 cut(s) 68
MseI TTAA 7 cut(s) 234, 399, 710, 747, 923, 1406, 1481
Msp20I TGGCCA 1 cut(s) 68
MspA1I CMGCKG 1 cut(s) 679
MspI CCGG 2 cut(s) 81, 226
MspR9I CCNGG 9 cut(s) 226, 227, 477, 770, 951, 1035, 1065, 1326, 1433
MunI CAATTG 2 cut(s) 735, 1308
Mva1269I GAATGC 2 cut(s) 178, 253
MvaI CCWGG 7 cut(s) 477, 770, 951, 1035, 1065, 1326, 1433
MwoI GCNNNNNNNGC 7 cut(s) 26, 35, 170, 359, 461, 1016, 1437
NciI CCSGG 2 cut(s) 226, 227
NcoI CCATGG 1 cut(s) 63
NdeII GATC 3 cut(s) 260, 560, 857
NlaIV GGNNCC 2 cut(s) 562, 1441
NmuCI GTSAC 2 cut(s) 833, 1067
NsiI ATGCAT 1 cut(s) 1336
NspI RCATGY 1 cut(s) 644
PaeI GCATGC 1 cut(s) 644
PagI TCATGA 1 cut(s) 295
PctI GAATGC 2 cut(s) 178, 253
PfeI GAWTC 2 cut(s) 356, 757
PflMI CCANNNNNTGG 2 cut(s) 974, 1313
PfoI TCCNGGA 1 cut(s) 1324
PkrI GCNGC 3 cut(s) 1012, 1015, 1018
PleI GAGTC 2 cut(s) 539, 1444
PpsI GAGTC 2 cut(s) 539, 1444
Psp124BI GAGCTC 1 cut(s) 1283
Psp6I CCWGG 7 cut(s) 475, 768, 949, 1033, 1063, 1324, 1431
PspFI CCCAGC 1 cut(s) 305
PspGI CCWGG 7 cut(s) 475, 768, 949, 1033, 1063, 1324, 1431
PspN4I GGNNCC 2 cut(s) 562, 1441
PspPI GGNCC 3 cut(s) 6, 503, 1400
PstNI CAGNNNCTG 1 cut(s) 500
PsuI RGATCY 3 cut(s) 260, 560, 857
RsaI GTAC 5 cut(s) 60, 283, 383, 1218, 1378
RsaNI GTAC 5 cut(s) 59, 282, 382, 1217, 1377
SacI GAGCTC 1 cut(s) 1283
SaqAI TTAA 7 cut(s) 234, 399, 710, 747, 923, 1406, 1481
SatI GCNGC 3 cut(s) 1011, 1014, 1017
Sau3AI GATC 3 cut(s) 260, 560, 857
Sau96I GGNCC 3 cut(s) 6, 503, 1400
ScaI AGTACT 1 cut(s) 283
SchI GAGTC 2 cut(s) 539, 1444
ScrFI CCNGG 9 cut(s) 226, 227, 477, 770, 951, 1035, 1065, 1326, 1433
SduI GDGCHC 3 cut(s) 457, 670, 1283
SfaNI GCATC 5 cut(s) 160, 619, 741, 1255, 1343
SfcI CTRYAG 1 cut(s) 960
SinI GGWCC 2 cut(s) 503, 1400
SmaI CCCGGG 1 cut(s) 227
SpeI ACTAGT 1 cut(s) 1462
SphI GCATGC 1 cut(s) 644
Sse9I AATT 8 cut(s) 202, 255, 342, 735, 744, 797, 924, 1308
SsiI CCGC 2 cut(s) 599, 679
SspMI CTAG 2 cut(s) 1145, 1463
SstI GAGCTC 1 cut(s) 1283
StyD4I CCNGG 9 cut(s) 224, 225, 475, 768, 949, 1033, 1063, 1324, 1431
StyI CCWWGG 4 cut(s) 63, 519, 1039, 1090
TaaI ACNGT 4 cut(s) 137, 590, 788, 1216
TaiI ACGT 1 cut(s) 316
TaqI TCGA 1 cut(s) 849
TasI AATT 8 cut(s) 202, 255, 342, 735, 744, 797, 924, 1308
TatI WGTACW 3 cut(s) 281, 1216, 1376
TfiI GAWTC 2 cut(s) 356, 757
Tru1I TTAA 7 cut(s) 234, 399, 710, 747, 923, 1406, 1481
Tru9I TTAA 7 cut(s) 234, 399, 710, 747, 923, 1406, 1481
TscAI CASTG 2 cut(s) 171, 1460
TseFI GTSAC 2 cut(s) 833, 1067
TseI GCWGC 3 cut(s) 1010, 1013, 1016
Tsp45I GTSAC 2 cut(s) 833, 1067
TspDTI ATGAA 4 cut(s) 315, 684, 1284, 1380
TspGWI ACGGA 1 cut(s) 85
TspMI CCCGGG 1 cut(s) 225
TspRI CASTG 2 cut(s) 171, 1460
Van91I CCANNNNNTGG 2 cut(s) 974, 1313
VneI GTGCAC 1 cut(s) 666
VpaK11BI GGWCC 2 cut(s) 503, 1400
XapI RAATTY 1 cut(s) 797
XceI RCATGY 1 cut(s) 644
XmaI CCCGGG 1 cut(s) 225
XspI CTAG 2 cut(s) 1145, 1463
ZrmI AGTACT 1 cut(s) 283
Zsp2I ATGCAT 1 cut(s) 1336
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.