RLG00000033603

Methionine gamma-lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0015573
Physical Location & Seq
Forward (+)
29548976 .. 29554444
5469 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033603

Sequence Viewer

Length: 1683 bp
ATGTGGGCCTTATCCTGCGCCACCTCTGCTTATCCTGCGCTCACTTTCAGAGCTTCTGTACCCAAGGCCACTACTCGTTCCGGCTCTCCCGTCTCGGTCTCAGTCCCTACCAATCACCATTATTATCAGAAAGACAGTCCTTTTGTTCCAGAGGTTGTGGAAGCAGTGGATTCCTTGCATTCAGAGTTCAGAGCTGTGGATAATTTGGTAGCACACAATACCACCCGGGTTCTTAAAGCTTTCCAGAATGCCCGAATTGGATCTCATCACTTTGCTGGAAGTACTGGCTATGGTCACGATGAAGCTGGGGGACGTGAAGCTCTTGACCAAGCTTTTGCAGAAATTGTAGGCGCTGAATCTGCAATAGTTCGCTCACAGTTTTTCTCAGGAACTCATGCTATAACCTGTGCTCTGTTTTCTTTTCTAAGGCCAGGAGATGAGCTTTTGGCAGTTGCTGGTCCACCCTATGACACCTTGGAGGAGGTTATCGGAAAGAGAGACTCTCATGGCATGGGATCCTTGATAGATTTTGGAGTGAAATACCGTGAAGTTCCACTTGCTGAAGATGGTAGCCTTGATTGGGATGCGCTTAGGCATGCTTTGAACCCTGAAACAAAATGTGCACTCATACAGCGGTCATGTGGTTATTCATGGCGTCAGAGTTTAAGTGTGGATGATATAAGGCGAGCAATTGAGATAATTAAGATGCAGAATCCTAACTGCCTGGTCATGGTGGATAATTGTTATGGTGAATTTGTGGAAAGCATTGAACCTCCAATGGTGGGTGCAGATTTAATTGCTGGCAGTTTGATAAAAAACCCTGGTGGAACTATTGCCCCGTGTGGTGGATATGTTGCAGGAAGAGAAAGATGGGTAAAAGCAGCAGCAGCTCGCCTGTCTGCACCTGGCCTTGGAGTTGATTGTGGCTCAACTCCTGGTCACATTATGAGAGCGTTTTTCCAAGGTTTGTTCCTTTCACCTCAAATGGTTGGCGAGGCTATCAAGGGGACTTTCCTAGTAGCTGAAGTTATGGCTTCCAAAGGGTATAAGGTCCAGCCCCTTCCTGGTGTTCCTCGCCATGACACAGTACAGGCCGTACAGCTTGGAAATCGTGAGCATCTTCTTGCTTTCTGTGAGGCTGTGCAGAGGAGCTCCCCAGTCGGTTCATTTACTAAGCCAATTGCTGGCACAACTCCTGGATATGCATCAGAGGTGATCTTTGCTGATGGAACATTTGTTGATGGGAGTACAAGTGAGCTTTCATGCGATGGACCATTAAGAGAGCCATTCGCTGTATTTTGCCAGGGGGGCTCCCATTGGACTCAGTGGGGACTAGTTCTTGGAGAGTCTCAAGATATGTCCTTGCTGCAATTGCTCAAAATACAGCAGCAGTGGCATTTCACATCATTTTTTTCGAAAGAAGGGAGCTACTATGTGAATCCCAGGAGACAAATGAGGATGCTGCTTCAAGGGGCAGACCGCAAACAATTTGTCAAGGTTGTCATGATTGATATTCAAAGTAGAACACTGATCTTGTCTCCACTTCCTTCCTTCCTTGGTCGCTCTCGATTAAACTCAAGCATATCTCCATCAGAAAACCAGGCGTCGATCTCTTCCTCTGCAACCAAAACCACTGACCCAGTTCTTGAGCCACCTCTGGGTGGTGGAAAGATTCAAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003962 GO:0004123 GO:0016740 GO:0016765 GO:0016829 GO:0016846
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Met_gamma_lyase

Protein Analysis

561

Amino Acids

60.56

Weight (kDa)

6.32

Isoelectric Point (pI)

44.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Met_gamma_lyase PF06838 54 - 446 9.6e-164 Methionine gamma-lyase
Cys_Met_Meta_PP PF01053 61 - 297 4.3e-09 Cys/Met metabolism PLP-dependent enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015573)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g20850 FvH4_3g20850 FvH4_3g20850
malus_domestica MD03G1220100.v1.1
prunus_persica Prupe.4G188800_v2.0.a1 Prupe.4G188800_v2.0.a1
pyrus_communis pycom03g16940
rosa_chinensis RchiOBHm_Chr5g0035161
rosa_laevigata RLG00000033603
rosa_multiflora Rmu_sc0000879.1_g000001 Rmu_sc0008176.1_g000007
rosa_roxburghii Rroxscaffold_1G00045250
rosa_rugosa Rorug05G0150400
rosa_samantha Rh5BG242800 Rh5DG251600
rosa_wichuraiana Rw5G022220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1184
AciI CCGC 2 cut(s) 634, 1480
AclWI GGATC 3 cut(s) 268, 510, 523
AcsI RAATTY 1 cut(s) 752
AcuI CTGAAG 2 cut(s) 582, 1044
AcyI GRCGYC 2 cut(s) 655, 1604
AfaI GTAC 5 cut(s) 60, 283, 1089, 1098, 1249
AfiI CCNNNNNNNGG 9 cut(s) 580, 730, 782, 845, 911, 1064, 1184, 1658, 1661
AgsI TTSAA 5 cut(s) 604, 770, 1469, 1517, 1676
AhlI ACTAGT 1 cut(s) 1333
AjiI CACGTC 1 cut(s) 314
Alw21I GWGCWC 3 cut(s) 412, 625, 1154
Alw26I GTCTC 6 cut(s) 97, 103, 492, 1353, 1441, 1542
Alw44I GTGCAC 1 cut(s) 621
AlwI GGATC 3 cut(s) 268, 510, 523
AlwNI CAGNNNCTG 1 cut(s) 455
Ama87I CYCGRG 1 cut(s) 225
AoxI GGCC 5 cut(s) 6, 66, 428, 907, 1092
ApaLI GTGCAC 1 cut(s) 621
ApeKI GCWGC 6 cut(s) 881, 884, 887, 1366, 1387, 1462
ApoI RAATTY 1 cut(s) 752
ArsI GACNNNNNNTTYG 6 cut(s) 125, 157, 317, 349, 1476, 1508
AspLEI GCGC 4 cut(s) 20, 40, 353, 589
AspS9I GGNCC 4 cut(s) 6, 458, 1051, 1271
AsuC2I CCSGG 2 cut(s) 226, 227
AsuHPI GGTGA 4 cut(s) 107, 761, 969, 1225
AsuII TTCGAA 1 cut(s) 1415
AvaI CYCGRG 1 cut(s) 225
AvaII GGWCC 3 cut(s) 458, 1051, 1271
BaeGI GKGCMC 1 cut(s) 625
BamHI GGATCC 1 cut(s) 515
BanII GRGCYC 2 cut(s) 1154, 1313
Bbv12I GWGCWC 3 cut(s) 412, 625, 1154
BbvI GCAGC 6 cut(s) 893, 896, 899, 1353, 1399, 1449
BccI CCATC 6 cut(s) 560, 864, 1220, 1235, 1262, 1597
BceAI ACGGC 1 cut(s) 1079
BcnI CCSGG 2 cut(s) 226, 227
BcoDI GTCTC 6 cut(s) 97, 103, 492, 1353, 1441, 1542
BcuI ACTAGT 1 cut(s) 1333
BfaI CTAG 2 cut(s) 1016, 1334
BfoI RGCGCY 1 cut(s) 354
BglI GCCNNNNNGGC 1 cut(s) 1308
BisI GCNGC 6 cut(s) 882, 885, 888, 1367, 1388, 1463
BlsI GCNGC 6 cut(s) 883, 886, 889, 1368, 1389, 1464
BmcAI AGTACT 1 cut(s) 283
Bme18I GGWCC 3 cut(s) 458, 1051, 1271
BmeT110I CYCGRG 1 cut(s) 225
BmgBI CACGTC 1 cut(s) 314
BmgT120I GGNCC 4 cut(s) 6, 458, 1051, 1271
BmiI GGNNCC 2 cut(s) 517, 1312
BmrI ACTGGG 2 cut(s) 1151, 1634
BmsI GCATC 5 cut(s) 574, 696, 1126, 1214, 1449
BmuI ACTGGG 2 cut(s) 1151, 1634
BplI GAGNNNNNCTC 2 cut(s) 487, 519
Bpu10I CCTNAGC 1 cut(s) 590
Bpu14I TTCGAA 1 cut(s) 1415
BpuEI CTTGAG 3 cut(s) 1335, 1561, 1667
BpuMI CCSGG 2 cut(s) 226, 227
BsaBI GATNNNNATC 1 cut(s) 1204
BsaHI GRCGYC 2 cut(s) 655, 1604
BsaI GGTCTC 1 cut(s) 103
BsaJI CCNNGG 9 cut(s) 63, 225, 474, 820, 910, 961, 1303, 1442, 1555
BsaXI ACNNNNNCTCC 2 cut(s) 1237, 1267
Bsc4I CCNNNNNNNGG 9 cut(s) 580, 730, 782, 845, 911, 1064, 1184, 1658, 1661
Bse1I ACTGG 3 cut(s) 289, 1157, 1640
Bse8I GATNNNNATC 1 cut(s) 1204
BseDI CCNNGG 9 cut(s) 63, 225, 474, 820, 910, 961, 1303, 1442, 1555
BseGI GGATG 3 cut(s) 589, 679, 1464
BseJI GATNNNNATC 1 cut(s) 1204
BseLI CCNNNNNNNGG 9 cut(s) 580, 730, 782, 845, 911, 1064, 1184, 1658, 1661
BseMII CTCAG 3 cut(s) 114, 399, 1337
BseNI ACTGG 3 cut(s) 289, 1157, 1640
BseRI GAGGAG 2 cut(s) 494, 1162
BseSI GKGCMC 1 cut(s) 625
BseXI GCAGC 6 cut(s) 893, 896, 899, 1353, 1399, 1449
BseYI CCCAGC 1 cut(s) 305
BsgI GTGCAG 3 cut(s) 807, 885, 1163
BshFI GGCC 5 cut(s) 8, 68, 430, 909, 1094
BsiHKAI GWGCWC 3 cut(s) 412, 625, 1154
BsiHKCI CYCGRG 1 cut(s) 225
BsiSI CCGG 2 cut(s) 81, 226
BslFI GGGAC 4 cut(s) 89, 324, 1021, 1344
BslI CCNNNNNNNGG 9 cut(s) 580, 730, 782, 845, 911, 1064, 1184, 1658, 1661
BsmAI GTCTC 6 cut(s) 97, 103, 492, 1353, 1441, 1542
BsmBI CGTCTC 1 cut(s) 97
BsmFI GGGAC 4 cut(s) 89, 324, 1021, 1344
BsmI GAATGC 2 cut(s) 178, 253
BsnI GGCC 5 cut(s) 8, 68, 430, 909, 1094
Bso31I GGTCTC 1 cut(s) 103
BsoBI CYCGRG 1 cut(s) 225
Bsp119I TTCGAA 1 cut(s) 1415
Bsp1286I GDGCHC 4 cut(s) 412, 625, 1154, 1313
Bsp143I GATC 5 cut(s) 260, 515, 1215, 1530, 1608
BspACI CCGC 2 cut(s) 634, 1480
BspANI GGCC 5 cut(s) 8, 68, 430, 909, 1094
BspCNI CTCAG 3 cut(s) 113, 398, 1336
BspHI TCATGA 1 cut(s) 1503
BspLI GGNNCC 2 cut(s) 517, 1312
BspPI GGATC 3 cut(s) 268, 510, 523
BspT104I TTCGAA 1 cut(s) 1415
BspTNI GGTCTC 1 cut(s) 103
BsrI ACTGG 3 cut(s) 289, 1157, 1640
BssECI CCNNGG 9 cut(s) 63, 225, 474, 820, 910, 961, 1303, 1442, 1555
BssMI GATC 5 cut(s) 260, 515, 1215, 1530, 1608
BssNI GRCGYC 2 cut(s) 655, 1604
BssT1I CCWWGG 5 cut(s) 63, 474, 910, 961, 1555
Bst4CI ACNGT 4 cut(s) 137, 378, 545, 1087
Bst6I CTCTTC 2 cut(s) 856, 1618
BstACI GRCGYC 2 cut(s) 655, 1604
BstBI TTCGAA 1 cut(s) 1415
BstC8I GCNNGC 5 cut(s) 597, 687, 802, 892, 1186
BstDEI CTNAG 6 cut(s) 100, 385, 425, 590, 1173, 1323
BstF5I GGATG 3 cut(s) 589, 679, 1464
BstH2I RGCGCY 1 cut(s) 354
BstHHI GCGC 4 cut(s) 20, 40, 353, 589
BstKTI GATC 5 cut(s) 263, 518, 1218, 1533, 1611
BstMAI GTCTC 6 cut(s) 97, 103, 492, 1353, 1441, 1542
BstMBI GATC 5 cut(s) 260, 515, 1215, 1530, 1608
BstMWI GCNNNNNNNGC 7 cut(s) 26, 35, 359, 887, 1308, 1372, 1393
BstNSI RCATGY 1 cut(s) 599
BstSLI GKGCMC 1 cut(s) 625
BstV1I GCAGC 6 cut(s) 893, 896, 899, 1353, 1399, 1449
BstX2I RGATCY 2 cut(s) 260, 515
BstYI RGATCY 2 cut(s) 260, 515
BsuRI GGCC 5 cut(s) 8, 68, 430, 909, 1094
BtgZI GCGATG 1 cut(s) 1281
BtrI CACGTC 1 cut(s) 314
BtsCI GGATG 3 cut(s) 589, 679, 1464
BtsI GCAGTG 2 cut(s) 171, 1397
BtsIMutI CAGTG 5 cut(s) 171, 1331, 1397, 1526, 1632
Cac8I GCNNGC 5 cut(s) 597, 687, 802, 892, 1186
CaiI CAGNNNCTG 1 cut(s) 455
CciI TCATGA 1 cut(s) 1503
CfoI GCGC 4 cut(s) 20, 40, 353, 589
Cfr13I GGNCC 4 cut(s) 6, 458, 1051, 1271
Cfr9I CCCGGG 1 cut(s) 225
CseI GACGC 2 cut(s) 644, 1593
Csp6I GTAC 5 cut(s) 59, 282, 1088, 1097, 1248
CviQI GTAC 5 cut(s) 59, 282, 1088, 1097, 1248
DdeI CTNAG 6 cut(s) 100, 385, 425, 590, 1173, 1323
DpnI GATC 5 cut(s) 262, 517, 1217, 1532, 1610
DpnII GATC 5 cut(s) 260, 515, 1215, 1530, 1608
Eam1104I CTCTTC 2 cut(s) 856, 1618
EarI CTCTTC 2 cut(s) 856, 1618
Ecl136II GAGCTC 1 cut(s) 1152
Eco130I CCWWGG 5 cut(s) 63, 474, 910, 961, 1555
Eco24I GRGCYC 2 cut(s) 1154, 1313
Eco31I GGTCTC 1 cut(s) 103
Eco47I GGWCC 3 cut(s) 458, 1051, 1271
Eco53kI GAGCTC 1 cut(s) 1152
Eco57I CTGAAG 2 cut(s) 582, 1044
Eco88I CYCGRG 1 cut(s) 225
EcoICRI GAGCTC 1 cut(s) 1152
EcoT14I CCWWGG 5 cut(s) 63, 474, 910, 961, 1555
EcoT22I ATGCAT 1 cut(s) 1207
EcoT38I GRGCYC 2 cut(s) 1154, 1313
ErhI CCWWGG 5 cut(s) 63, 474, 910, 961, 1555
Esp3I CGTCTC 1 cut(s) 97
FalI AAGNNNNNCTT 2 cut(s) 540, 572
FaqI GGGAC 4 cut(s) 89, 324, 1021, 1344
Fnu4HI GCNGC 6 cut(s) 882, 885, 888, 1367, 1388, 1463
FokI GGATG 3 cut(s) 596, 686, 1471
FriOI GRGCYC 2 cut(s) 1154, 1313
Fsp4HI GCNGC 6 cut(s) 882, 885, 888, 1367, 1388, 1463
FspBI CTAG 2 cut(s) 1016, 1334
GlaI GCGC 4 cut(s) 19, 39, 352, 588
GluI GCNGC 6 cut(s) 882, 885, 888, 1367, 1388, 1463
GsaI CCCAGC 1 cut(s) 309
HaeII RGCGCY 1 cut(s) 354
HaeIII GGCC 5 cut(s) 8, 68, 430, 909, 1094
HapII CCGG 2 cut(s) 81, 226
HgaI GACGC 2 cut(s) 644, 1593
HhaI GCGC 4 cut(s) 20, 40, 353, 589
Hin1I GRCGYC 2 cut(s) 655, 1604
Hin6I GCGC 4 cut(s) 18, 38, 351, 587
HinP1I GCGC 4 cut(s) 18, 38, 351, 587
HindIII AAGCTT 2 cut(s) 237, 330
HinfI GANTC 8 cut(s) 170, 356, 500, 712, 1321, 1346, 1438, 1672
HpaII CCGG 2 cut(s) 81, 226
HphI GGTGA 4 cut(s) 107, 761, 969, 1225
Hpy166II GTNNAC 2 cut(s) 461, 623
Hpy188I TCNGA 8 cut(s) 50, 129, 184, 191, 491, 660, 1210, 1594
Hpy8I GTNNAC 2 cut(s) 461, 623
Hpy99I CGWCG 1 cut(s) 1609
HpyAV CCTTC 4 cut(s) 1070, 1415, 1557, 1561
HpyCH4III ACNGT 4 cut(s) 137, 378, 545, 1087
HpyCH4IV ACGT 1 cut(s) 313
HpyF10VI GCNNNNNNNGC 7 cut(s) 26, 35, 359, 887, 1308, 1372, 1393
HpyF3I CTNAG 6 cut(s) 100, 385, 425, 590, 1173, 1323
HpySE526I ACGT 1 cut(s) 313
Hsp92I GRCGYC 2 cut(s) 655, 1604
HspAI GCGC 4 cut(s) 18, 38, 351, 587
Kzo9I GATC 5 cut(s) 260, 515, 1215, 1530, 1608
LmnI GCTCC 4 cut(s) 1149, 1157, 1316, 1425
Lsp1109I GCAGC 6 cut(s) 893, 896, 899, 1353, 1399, 1449
LweI GCATC 5 cut(s) 574, 696, 1126, 1214, 1449
MaeI CTAG 2 cut(s) 1016, 1334
MaeII ACGT 1 cut(s) 313
MaeIII GTNAC 2 cut(s) 293, 938
MalI GATC 5 cut(s) 262, 517, 1217, 1532, 1610
MboI GATC 5 cut(s) 260, 515, 1215, 1530, 1608
MboII GAAGA 4 cut(s) 575, 873, 1112, 1605
MfeI CAATTG 3 cut(s) 690, 1179, 1370
MflI RGATCY 2 cut(s) 260, 515
MhlI GDGCHC 4 cut(s) 412, 625, 1154, 1313
MlyI GAGTC 3 cut(s) 494, 1315, 1355
Mph1103I ATGCAT 1 cut(s) 1207
MseI TTAA 6 cut(s) 234, 665, 702, 794, 1277, 1571
MspA1I CMGCKG 1 cut(s) 634
MspI CCGG 2 cut(s) 81, 226
MunI CAATTG 3 cut(s) 690, 1179, 1370
Mva1269I GAATGC 2 cut(s) 178, 253
MwoI GCNNNNNNNGC 7 cut(s) 26, 35, 359, 887, 1308, 1372, 1393
NciI CCSGG 2 cut(s) 226, 227
NdeII GATC 5 cut(s) 260, 515, 1215, 1530, 1608
NlaIV GGNNCC 2 cut(s) 517, 1312
NmuCI GTSAC 2 cut(s) 293, 938
NsiI ATGCAT 1 cut(s) 1207
NspI RCATGY 1 cut(s) 599
NspV TTCGAA 1 cut(s) 1415
PaeI GCATGC 1 cut(s) 599
PagI TCATGA 1 cut(s) 1503
PctI GAATGC 2 cut(s) 178, 253
PfeI GAWTC 5 cut(s) 170, 356, 712, 1438, 1672
PflMI CCANNNNNTGG 1 cut(s) 1184
PfoI TCCNGGA 1 cut(s) 1195
PkrI GCNGC 6 cut(s) 883, 886, 889, 1368, 1389, 1464
PleI GAGTC 3 cut(s) 494, 1315, 1354
PpsI GAGTC 3 cut(s) 494, 1315, 1354
Psp124BI GAGCTC 1 cut(s) 1154
PspFI CCCAGC 1 cut(s) 305
PspN4I GGNNCC 2 cut(s) 517, 1312
PspPI GGNCC 4 cut(s) 6, 458, 1051, 1271
PstNI CAGNNNCTG 1 cut(s) 455
PsuI RGATCY 2 cut(s) 260, 515
RsaI GTAC 5 cut(s) 60, 283, 1089, 1098, 1249
RsaNI GTAC 5 cut(s) 59, 282, 1088, 1097, 1248
SacI GAGCTC 1 cut(s) 1154
SaqAI TTAA 6 cut(s) 234, 665, 702, 794, 1277, 1571
SatI GCNGC 6 cut(s) 882, 885, 888, 1367, 1388, 1463
Sau3AI GATC 5 cut(s) 260, 515, 1215, 1530, 1608
Sau96I GGNCC 4 cut(s) 6, 458, 1051, 1271
ScaI AGTACT 1 cut(s) 283
SchI GAGTC 3 cut(s) 494, 1315, 1355
SduI GDGCHC 4 cut(s) 412, 625, 1154, 1313
SfaNI GCATC 5 cut(s) 574, 696, 1126, 1214, 1449
SfuI TTCGAA 1 cut(s) 1415
SinI GGWCC 3 cut(s) 458, 1051, 1271
SmaI CCCGGG 1 cut(s) 227
SmlI CTYRAG 3 cut(s) 1350, 1576, 1646
SmoI CTYRAG 3 cut(s) 1350, 1576, 1646
SpeI ACTAGT 1 cut(s) 1333
SphI GCATGC 1 cut(s) 599
SsiI CCGC 2 cut(s) 634, 1480
SspMI CTAG 2 cut(s) 1016, 1334
SstI GAGCTC 1 cut(s) 1154
StyI CCWWGG 5 cut(s) 63, 474, 910, 961, 1555
TaaI ACNGT 4 cut(s) 137, 378, 545, 1087
TaiI ACGT 1 cut(s) 316
TaqI TCGA 3 cut(s) 1415, 1567, 1607
TaqII GACCGA 1 cut(s) 85
TatI WGTACW 3 cut(s) 281, 1087, 1247
TfiI GAWTC 5 cut(s) 170, 356, 712, 1438, 1672
Tru1I TTAA 6 cut(s) 234, 665, 702, 794, 1277, 1571
Tru9I TTAA 6 cut(s) 234, 665, 702, 794, 1277, 1571
TscAI CASTG 5 cut(s) 171, 1331, 1397, 1533, 1639
TseFI GTSAC 2 cut(s) 293, 938
TseI GCWGC 6 cut(s) 881, 884, 887, 1366, 1387, 1462
Tsp45I GTSAC 2 cut(s) 293, 938
TspDTI ATGAA 4 cut(s) 315, 639, 1155, 1251
TspMI CCCGGG 1 cut(s) 225
TspRI CASTG 5 cut(s) 171, 1331, 1397, 1533, 1639
Van91I CCANNNNNTGG 1 cut(s) 1184
VneI GTGCAC 1 cut(s) 621
VpaK11BI GGWCC 3 cut(s) 458, 1051, 1271
XapI RAATTY 1 cut(s) 752
XceI RCATGY 1 cut(s) 599
XcmI CCANNNNNNNNNTGG 1 cut(s) 1061
XmaI CCCGGG 1 cut(s) 225
XspI CTAG 2 cut(s) 1016, 1334
ZrmI AGTACT 1 cut(s) 283
Zsp2I ATGCAT 1 cut(s) 1207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.