Rmu_sc0000879.1_g000001

Methionine gamma-lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000879.1
OG0015573
Physical Location & Seq
Reverse (-)
1662 .. 5606
3945 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000879.1_g000001.1.cds

Sequence Viewer

Length: 1215 bp
atgtgggccttatcctgcgccacctctgcatatcctgcgctcactttcagagcttctgtacccatggccactactcgttccggctctcccttctccgtctcagtccctaccaatcaccattattatcagaaagacagcccttttgttccagaggttgtggaagcagtggattccttgcattcagagttcagagctgtggataatttggtagcatgcaacaccacccgggttcttaaagctttccagaatgcccgaattggatctcatcactttgctggaagtactggctatggtcatgatgaagctgggggacgtgaagctcttgaccaagcttttgcagaaattgtaggcgctgaatctgcagtagttcgctcacagtttttctcaggaactcatgccataacctgcgctctgtttgcttttctcaggccaggagatgagcttttggcagttgctggtcctccctatgacaccttggaggaggttattggaaagagagactctcatggcatgggatccttgatagactttggagtgaaataccgggaagttccaatgcagaatcctaactgcctggtcatggtggataattgttatggtgaatttgtggaaagcattgaacctccaatggtgggtgcagatttaattgctggcagtttgataaaaaatcctggtggaactatagccccatgcggtggatatgttgcaggaagagaaagatgggtaaaagcagcagcagctcgtctgtctgcacctggccttggagttgattctggctcaactcctggtcacattatgagagcatttttccaaggtttgttcctttcacctcaaatggttggcgaggctatcaaggggactttcctagtagctgaagttatggcttccaaagggtataaggtccagccccttcctcgtgttcctcgccatgacacagtacaggccatacagcttggaaatcgtgagcgtcttctttctttctgtgaggctgtgcagaggagctccccagtcggttcatttactaaaccaattgctggcacaactcctggatatgcatcagaggtgatctttgctgatggaacatttgttgatgggagtacaagtgagctttcatgcgatggaccattaagagagccattcgctgtattttgccagggtggctcccattggactcagtggggactagttcttggagaggttttaaaagccatataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003962 GO:0004123 GO:0016740 GO:0016765 GO:0016829 GO:0016846
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Met_gamma_lyase

Protein Analysis

404

Amino Acids

43.06

Weight (kDa)

5.78

Isoelectric Point (pI)

34.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015573)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g20850 FvH4_3g20850 FvH4_3g20850
malus_domestica MD03G1220100.v1.1
prunus_persica Prupe.4G188800_v2.0.a1 Prupe.4G188800_v2.0.a1
pyrus_communis pycom03g16940
rosa_chinensis RchiOBHm_Chr5g0035161
rosa_laevigata RLG00000033603
rosa_multiflora Rmu_sc0000879.1_g000001 Rmu_sc0008176.1_g000007
rosa_roxburghii Rroxscaffold_1G00045250
rosa_rugosa Rorug05G0150400
rosa_samantha Rh5BG242800 Rh5DG251600
rosa_wichuraiana Rw5G022220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 413
AccB7I CCANNNNNTGG 2 cut(s) 695, 1034
AciI CCGC 1 cut(s) 693
AclWI GGATC 3 cut(s) 268, 510, 523
AcoI YGGCCR 1 cut(s) 66
AcsI RAATTY 1 cut(s) 602
AcuI CTGAAG 1 cut(s) 894
AfaI GTAC 4 cut(s) 60, 283, 939, 1099
AfiI CCNNNNNNNGG 5 cut(s) 580, 632, 695, 761, 1034
AgsI TTSAA 1 cut(s) 620
AhlI ACTAGT 1 cut(s) 1183
AjiI CACGTC 1 cut(s) 314
AjnI CCWGG 7 cut(s) 430, 573, 670, 754, 784, 1045, 1152
Alw21I GWGCWC 1 cut(s) 1004
Alw26I GTCTC 2 cut(s) 103, 492
AlwI GGATC 3 cut(s) 268, 510, 523
AlwNI CAGNNNCTG 1 cut(s) 455
Ama87I CYCGRG 1 cut(s) 225
AoxI GGCC 5 cut(s) 6, 66, 428, 757, 942
ApeKI GCWGC 3 cut(s) 731, 734, 737
ApoI RAATTY 1 cut(s) 602
ArsI GACNNNNNNTTYG 4 cut(s) 125, 157, 317, 349
AspLEI GCGC 4 cut(s) 20, 40, 353, 410
AspS9I GGNCC 4 cut(s) 6, 458, 901, 1121
AsuC2I CCSGG 3 cut(s) 226, 227, 545
AsuHPI GGTGA 4 cut(s) 107, 611, 819, 1075
AvaI CYCGRG 1 cut(s) 225
AvaII GGWCC 3 cut(s) 458, 901, 1121
BalI TGGCCA 1 cut(s) 68
BamHI GGATCC 1 cut(s) 515
BanII GRGCYC 1 cut(s) 1004
BauI CACGAG 1 cut(s) 915
BbsI GAAGAC 1 cut(s) 962
Bbv12I GWGCWC 1 cut(s) 1004
BbvI GCAGC 3 cut(s) 743, 746, 749
BccI CCATC 4 cut(s) 714, 1070, 1085, 1112
BciT130I CCWGG 7 cut(s) 432, 575, 672, 756, 786, 1047, 1154
BcnI CCSGG 3 cut(s) 226, 227, 545
BcoDI GTCTC 2 cut(s) 103, 492
BcuI ACTAGT 1 cut(s) 1183
BfaI CTAG 2 cut(s) 866, 1184
BfmI CTRYAG 2 cut(s) 360, 681
BfoI RGCGCY 1 cut(s) 354
BfuAI ACCTGC 1 cut(s) 413
BglI GCCNNNNNGGC 1 cut(s) 1158
BisI GCNGC 3 cut(s) 732, 735, 738
BlsI GCNGC 3 cut(s) 733, 736, 739
BmcAI AGTACT 1 cut(s) 283
Bme18I GGWCC 3 cut(s) 458, 901, 1121
BmeT110I CYCGRG 1 cut(s) 225
BmgBI CACGTC 1 cut(s) 314
BmgT120I GGNCC 4 cut(s) 6, 458, 901, 1121
BmiI GGNNCC 2 cut(s) 517, 1162
BmrI ACTGGG 1 cut(s) 1001
BmsI GCATC 1 cut(s) 1064
BmuI ACTGGG 1 cut(s) 1001
BpiI GAAGAC 1 cut(s) 962
BplI GAGNNNNNCTC 2 cut(s) 487, 519
BpuMI CCSGG 3 cut(s) 226, 227, 545
BsaBI GATNNNNATC 1 cut(s) 1054
BsaJI CCNNGG 6 cut(s) 63, 225, 474, 760, 811, 1153
BsaXI ACNNNNNCTCC 2 cut(s) 1087, 1117
Bsc4I CCNNNNNNNGG 5 cut(s) 580, 632, 695, 761, 1034
Bse1I ACTGG 2 cut(s) 289, 1007
Bse8I GATNNNNATC 1 cut(s) 1054
BseBI CCWGG 7 cut(s) 432, 575, 672, 756, 786, 1047, 1154
BseDI CCNNGG 6 cut(s) 63, 225, 474, 760, 811, 1153
BseJI GATNNNNATC 1 cut(s) 1054
BseLI CCNNNNNNNGG 5 cut(s) 580, 632, 695, 761, 1034
BseMII CTCAG 4 cut(s) 114, 399, 439, 1187
BseNI ACTGG 2 cut(s) 289, 1007
BseRI GAGGAG 2 cut(s) 494, 1012
BseXI GCAGC 3 cut(s) 743, 746, 749
BseYI CCCAGC 1 cut(s) 305
BsgI GTGCAG 3 cut(s) 657, 735, 1013
BshFI GGCC 5 cut(s) 8, 68, 430, 759, 944
BsiHKAI GWGCWC 1 cut(s) 1004
BsiHKCI CYCGRG 1 cut(s) 225
BsiSI CCGG 3 cut(s) 81, 226, 544
BslFI GGGAC 4 cut(s) 89, 324, 871, 1194
BslI CCNNNNNNNGG 5 cut(s) 580, 632, 695, 761, 1034
BsmAI GTCTC 2 cut(s) 103, 492
BsmBI CGTCTC 1 cut(s) 103
BsmFI GGGAC 4 cut(s) 89, 324, 871, 1194
BsmI GAATGC 2 cut(s) 178, 253
BsnI GGCC 5 cut(s) 8, 68, 430, 759, 944
BsoBI CYCGRG 1 cut(s) 225
Bsp1286I GDGCHC 1 cut(s) 1004
Bsp143I GATC 3 cut(s) 260, 515, 1065
Bsp19I CCATGG 1 cut(s) 63
BspACI CCGC 1 cut(s) 693
BspANI GGCC 5 cut(s) 8, 68, 430, 759, 944
BspCNI CTCAG 4 cut(s) 113, 398, 438, 1186
BspHI TCATGA 1 cut(s) 295
BspLI GGNNCC 2 cut(s) 517, 1162
BspMAI CTGCAG 1 cut(s) 364
BspMI ACCTGC 1 cut(s) 413
BspPI GGATC 3 cut(s) 268, 510, 523
BsrI ACTGG 2 cut(s) 289, 1007
BssECI CCNNGG 6 cut(s) 63, 225, 474, 760, 811, 1153
BssMI GATC 3 cut(s) 260, 515, 1065
BssSI CACGAG 1 cut(s) 915
BssT1I CCWWGG 4 cut(s) 63, 474, 760, 811
Bst2BI CACGAG 1 cut(s) 915
Bst2UI CCWGG 7 cut(s) 432, 575, 672, 756, 786, 1047, 1154
Bst4CI ACNGT 2 cut(s) 378, 937
Bst6I CTCTTC 1 cut(s) 706
BstAPI GCANNNNNTGC 1 cut(s) 35
BstC8I GCNNGC 3 cut(s) 214, 652, 1036
BstDEI CTNAG 4 cut(s) 100, 385, 425, 1173
BstDSI CCRYGG 1 cut(s) 63
BstH2I RGCGCY 1 cut(s) 354
BstHHI GCGC 4 cut(s) 20, 40, 353, 410
BstKTI GATC 3 cut(s) 263, 518, 1068
BstMAI GTCTC 2 cut(s) 103, 492
BstMBI GATC 3 cut(s) 260, 515, 1065
BstMWI GCNNNNNNNGC 6 cut(s) 26, 35, 359, 416, 737, 1158
BstNI CCWGG 7 cut(s) 432, 575, 672, 756, 786, 1047, 1154
BstNSI RCATGY 1 cut(s) 216
BstSFI CTRYAG 2 cut(s) 360, 681
BstV1I GCAGC 3 cut(s) 743, 746, 749
BstV2I GAAGAC 1 cut(s) 962
BstX2I RGATCY 2 cut(s) 260, 515
BstYI RGATCY 2 cut(s) 260, 515
BsuRI GGCC 5 cut(s) 8, 68, 430, 759, 944
BtgI CCRYGG 1 cut(s) 63
BtgZI GCGATG 1 cut(s) 1131
BtrI CACGTC 1 cut(s) 314
BtsI GCAGTG 1 cut(s) 171
BtsIMutI CAGTG 2 cut(s) 171, 1181
BveI ACCTGC 1 cut(s) 413
Cac8I GCNNGC 3 cut(s) 214, 652, 1036
CaiI CAGNNNCTG 1 cut(s) 455
CciI TCATGA 1 cut(s) 295
CfoI GCGC 4 cut(s) 20, 40, 353, 410
Cfr13I GGNCC 4 cut(s) 6, 458, 901, 1121
Cfr9I CCCGGG 1 cut(s) 225
CseI GACGC 1 cut(s) 956
Csp6I GTAC 4 cut(s) 59, 282, 938, 1098
CviQI GTAC 4 cut(s) 59, 282, 938, 1098
DdeI CTNAG 4 cut(s) 100, 385, 425, 1173
DpnI GATC 3 cut(s) 262, 517, 1067
DpnII GATC 3 cut(s) 260, 515, 1065
DraI TTTAAA 1 cut(s) 1203
EaeI YGGCCR 1 cut(s) 66
Eam1104I CTCTTC 1 cut(s) 706
EarI CTCTTC 1 cut(s) 706
Ecl136II GAGCTC 1 cut(s) 1002
Eco130I CCWWGG 4 cut(s) 63, 474, 760, 811
Eco24I GRGCYC 1 cut(s) 1004
Eco47I GGWCC 3 cut(s) 458, 901, 1121
Eco53kI GAGCTC 1 cut(s) 1002
Eco57I CTGAAG 1 cut(s) 894
Eco88I CYCGRG 1 cut(s) 225
EcoICRI GAGCTC 1 cut(s) 1002
EcoRII CCWGG 7 cut(s) 430, 573, 670, 754, 784, 1045, 1152
EcoT14I CCWWGG 4 cut(s) 63, 474, 760, 811
EcoT22I ATGCAT 1 cut(s) 1057
EcoT38I GRGCYC 1 cut(s) 1004
ErhI CCWWGG 4 cut(s) 63, 474, 760, 811
Esp3I CGTCTC 1 cut(s) 103
FaqI GGGAC 4 cut(s) 89, 324, 871, 1194
Fnu4HI GCNGC 3 cut(s) 732, 735, 738
FriOI GRGCYC 1 cut(s) 1004
Fsp4HI GCNGC 3 cut(s) 732, 735, 738
FspBI CTAG 2 cut(s) 866, 1184
GlaI GCGC 4 cut(s) 19, 39, 352, 409
GluI GCNGC 3 cut(s) 732, 735, 738
GsaI CCCAGC 1 cut(s) 309
HaeII RGCGCY 1 cut(s) 354
HaeIII GGCC 5 cut(s) 8, 68, 430, 759, 944
HapII CCGG 3 cut(s) 81, 226, 544
HgaI GACGC 1 cut(s) 956
HhaI GCGC 4 cut(s) 20, 40, 353, 410
Hin6I GCGC 4 cut(s) 18, 38, 351, 408
HinP1I GCGC 4 cut(s) 18, 38, 351, 408
HindIII AAGCTT 2 cut(s) 237, 330
HinfI GANTC 6 cut(s) 170, 356, 500, 562, 770, 1171
HpaII CCGG 3 cut(s) 81, 226, 544
HphI GGTGA 4 cut(s) 107, 611, 819, 1075
Hpy188I TCNGA 5 cut(s) 50, 129, 184, 191, 1060
Hpy188III TCNNGA 6 cut(s) 149, 244, 296, 323, 387, 962
HpyAV CCTTC 2 cut(s) 100, 920
HpyCH4III ACNGT 2 cut(s) 378, 937
HpyCH4IV ACGT 1 cut(s) 313
HpyF10VI GCNNNNNNNGC 6 cut(s) 26, 35, 359, 416, 737, 1158
HpyF3I CTNAG 4 cut(s) 100, 385, 425, 1173
HpySE526I ACGT 1 cut(s) 313
HspAI GCGC 4 cut(s) 18, 38, 351, 408
Kzo9I GATC 3 cut(s) 260, 515, 1065
LmnI GCTCC 3 cut(s) 999, 1007, 1166
Lsp1109I GCAGC 3 cut(s) 743, 746, 749
LweI GCATC 1 cut(s) 1064
MaeI CTAG 2 cut(s) 866, 1184
MaeII ACGT 1 cut(s) 313
MaeIII GTNAC 1 cut(s) 788
MalI GATC 3 cut(s) 262, 517, 1067
MboI GATC 3 cut(s) 260, 515, 1065
MboII GAAGA 2 cut(s) 723, 962
MfeI CAATTG 1 cut(s) 1029
MflI RGATCY 2 cut(s) 260, 515
MhlI GDGCHC 1 cut(s) 1004
MlsI TGGCCA 1 cut(s) 68
MluCI AATT 7 cut(s) 202, 255, 342, 589, 602, 645, 1029
MluNI TGGCCA 1 cut(s) 68
MlyI GAGTC 2 cut(s) 494, 1165
Mox20I TGGCCA 1 cut(s) 68
Mph1103I ATGCAT 1 cut(s) 1057
MscI TGGCCA 1 cut(s) 68
MseI TTAA 4 cut(s) 234, 644, 1127, 1202
Msp20I TGGCCA 1 cut(s) 68
MspI CCGG 3 cut(s) 81, 226, 544
MunI CAATTG 1 cut(s) 1029
Mva1269I GAATGC 2 cut(s) 178, 253
MvaI CCWGG 7 cut(s) 432, 575, 672, 756, 786, 1047, 1154
MwoI GCNNNNNNNGC 6 cut(s) 26, 35, 359, 416, 737, 1158
NciI CCSGG 3 cut(s) 226, 227, 545
NcoI CCATGG 1 cut(s) 63
NdeII GATC 3 cut(s) 260, 515, 1065
NlaIV GGNNCC 2 cut(s) 517, 1162
NmuCI GTSAC 1 cut(s) 788
NsiI ATGCAT 1 cut(s) 1057
NspI RCATGY 1 cut(s) 216
PaeI GCATGC 1 cut(s) 216
PagI TCATGA 1 cut(s) 295
PctI GAATGC 2 cut(s) 178, 253
PfeI GAWTC 4 cut(s) 170, 356, 562, 770
PflMI CCANNNNNTGG 2 cut(s) 695, 1034
PfoI TCCNGGA 1 cut(s) 1045
PkrI GCNGC 3 cut(s) 733, 736, 739
PleI GAGTC 2 cut(s) 494, 1165
PpsI GAGTC 2 cut(s) 494, 1165
Psp124BI GAGCTC 1 cut(s) 1004
Psp6I CCWGG 7 cut(s) 430, 573, 670, 754, 784, 1045, 1152
PspFI CCCAGC 1 cut(s) 305
PspGI CCWGG 7 cut(s) 430, 573, 670, 754, 784, 1045, 1152
PspN4I GGNNCC 2 cut(s) 517, 1162
PspPI GGNCC 4 cut(s) 6, 458, 901, 1121
PstI CTGCAG 1 cut(s) 364
PstNI CAGNNNCTG 1 cut(s) 455
PsuI RGATCY 2 cut(s) 260, 515
RsaI GTAC 4 cut(s) 60, 283, 939, 1099
RsaNI GTAC 4 cut(s) 59, 282, 938, 1098
SacI GAGCTC 1 cut(s) 1004
SaqAI TTAA 4 cut(s) 234, 644, 1127, 1202
SatI GCNGC 3 cut(s) 732, 735, 738
Sau3AI GATC 3 cut(s) 260, 515, 1065
Sau96I GGNCC 4 cut(s) 6, 458, 901, 1121
ScaI AGTACT 1 cut(s) 283
SchI GAGTC 2 cut(s) 494, 1165
SduI GDGCHC 1 cut(s) 1004
SfaNI GCATC 1 cut(s) 1064
SfcI CTRYAG 2 cut(s) 360, 681
SinI GGWCC 3 cut(s) 458, 901, 1121
SmaI CCCGGG 1 cut(s) 227
SpeI ACTAGT 1 cut(s) 1183
SphI GCATGC 1 cut(s) 216
Sse9I AATT 7 cut(s) 202, 255, 342, 589, 602, 645, 1029
SsiI CCGC 1 cut(s) 693
SspMI CTAG 2 cut(s) 866, 1184
SstI GAGCTC 1 cut(s) 1004
StyI CCWWGG 4 cut(s) 63, 474, 760, 811
TaaI ACNGT 2 cut(s) 378, 937
TaiI ACGT 1 cut(s) 316
TasI AATT 7 cut(s) 202, 255, 342, 589, 602, 645, 1029
TatI WGTACW 3 cut(s) 281, 937, 1097
TfiI GAWTC 4 cut(s) 170, 356, 562, 770
Tru1I TTAA 4 cut(s) 234, 644, 1127, 1202
Tru9I TTAA 4 cut(s) 234, 644, 1127, 1202
TscAI CASTG 2 cut(s) 171, 1181
TseFI GTSAC 1 cut(s) 788
TseI GCWGC 3 cut(s) 731, 734, 737
Tsp45I GTSAC 1 cut(s) 788
TspDTI ATGAA 3 cut(s) 315, 1005, 1101
TspGWI ACGGA 1 cut(s) 85
TspMI CCCGGG 1 cut(s) 225
TspRI CASTG 2 cut(s) 171, 1181
Van91I CCANNNNNTGG 2 cut(s) 695, 1034
VpaK11BI GGWCC 3 cut(s) 458, 901, 1121
XapI RAATTY 1 cut(s) 602
XceI RCATGY 1 cut(s) 216
XmaI CCCGGG 1 cut(s) 225
XspI CTAG 2 cut(s) 866, 1184
ZrmI AGTACT 1 cut(s) 283
Zsp2I ATGCAT 1 cut(s) 1057
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.