Rh5BG242800

Methionine gamma-lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0015573
Physical Location & Seq
Forward (+)
29339883 .. 29344307
4425 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG242800.1

Sequence Viewer

Length: 1227 bp
ATGTGGGCCTTATCCTGCGCCACCTCTGCATATCCTGCGCTCACTTTCAGAGCTTCTGTACCCATGGCCACTACTCGTTCCGGCTCTCCCTTCTCCGTCTCAGTCCCTACCAATCACCATTATTATCAGAAAGACAGCCCTTTTGTTCCAGAGGTTGTGGAAGCAGTGGATTCCTTGCATTCAGAGTTCAGAGCAGTGGATAATTTGGTAGCATGCAACACCACCCGGGTTCTTAAAGCTTTCCAGAATGCCCGAATTGGATCTCATCACTTTACTGGGAGTACTGGCTATGGTCATGATGAAGCTGGGGGACGTGAAGCTCTTGACCAAGCTTTTGCAGAAATTGTAGGCGCTGAATCTGCAGTAGTTCGCTCACAGTTTTTCTCAGGAACTCATGCTATAACCTGCGCTCTGTTTGCTTTTCTCAGGCCAGGAGATGAGCTTTTGGCAGTTGCTGGTCCTCCCTATGACACCTTGGAGGAGGTTATTGGAAAGAGAGACTCTCATGGCATGGGATCCTTGATAGACTTTGGAGTGAAATACCGGGAAGTTCCACTTGCTGAAGATGGTGGCCTTGACTGGGATGCGCTTAGGCATGCTTTGAACCCTGAAACAAAATGTGCACTCATACAGCGTTCATGTGGTTATTCATGGCGTCAGAGTTTAAGTGTGGATGATATAAGGCGAGCAATTGAGATAATTAAGATGCAGAATCCTAACTGCCTGGTCATGGTGGATAATTGTTATGGTGAATTTGTGGAAAGCATTGAACCTCCAATGGTGGGTGCAGATTTAATTGCTGGCAGTTTGATAAAAAATCCTGGTGGAACTATAGCCCCATGCGGTGGATATGTTGCAGGAAGAGAAAGATGGGTAAAAGCAGCAGCAGCTCGTCTGTCTGCACCTGGCCTTGGAGTTGATTCTGGCTCAACTCCTGGTCACATTATGAGAGCATTTTTCCAAGGTTTGTTCCTTTCACCTCAAATGGTTGGCGAGGCTATCAAGGGGACTTTCCTAGTAGCAGAAGTTATGGCTTCCAAAGGCTATAAGGTCCAGCCCCTTCCTCGTGTTCCTCGCCATGACACAGTACAGGCCATACAGCTTGGAAATCGTGAGCGTCTTCTTGCTTTCTGTGAGGCTGTGCAGAGGAGCTCCCCAGTCGGTTCATTTACTAAACCAATTGCTGGCACAACTCCTGGATATGCATCAGAGGTCTGGCCACATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003962 GO:0004123 GO:0016740 GO:0016765 GO:0016829 GO:0016846
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Met_gamma_lyase

Protein Analysis

408

Amino Acids

43.93

Weight (kDa)

6.19

Isoelectric Point (pI)

40.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Met_gamma_lyase PF06838 54 - 405 5.8e-143 Methionine gamma-lyase
Cys_Met_Meta_PP PF01053 70 - 297 3.3e-09 Cys/Met metabolism PLP-dependent enzyme
Aminotran_1_2 PF00155 102 - 308 5.9e-06 Aminotransferase class I and II
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015573)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g20850 FvH4_3g20850 FvH4_3g20850
malus_domestica MD03G1220100.v1.1
prunus_persica Prupe.4G188800_v2.0.a1 Prupe.4G188800_v2.0.a1
pyrus_communis pycom03g16940
rosa_chinensis RchiOBHm_Chr5g0035161
rosa_laevigata RLG00000033603
rosa_multiflora Rmu_sc0000879.1_g000001 Rmu_sc0008176.1_g000007
rosa_roxburghii Rroxscaffold_1G00045250
rosa_rugosa Rorug05G0150400
rosa_samantha Rh5BG242800 Rh5DG251600
rosa_wichuraiana Rw5G022220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 413
AccB7I CCANNNNNTGG 2 cut(s) 845, 1184
AciI CCGC 1 cut(s) 843
AclWI GGATC 3 cut(s) 268, 510, 523
AcoI YGGCCR 2 cut(s) 66, 1217
AcsI RAATTY 1 cut(s) 752
AcuI CTGAAG 1 cut(s) 582
AcyI GRCGYC 1 cut(s) 655
AfaI GTAC 3 cut(s) 60, 283, 1089
AfiI CCNNNNNNNGG 6 cut(s) 580, 730, 782, 845, 911, 1184
AgsI TTSAA 2 cut(s) 604, 770
AjiI CACGTC 1 cut(s) 314
AjnI CCWGG 6 cut(s) 430, 723, 820, 904, 934, 1195
AluBI AGCT 9 cut(s) 53, 239, 305, 320, 332, 442, 890, 1102, 1152
AluI AGCT 9 cut(s) 53, 239, 305, 320, 332, 442, 890, 1102, 1152
Alw21I GWGCWC 2 cut(s) 625, 1154
Alw26I GTCTC 2 cut(s) 103, 492
Alw44I GTGCAC 1 cut(s) 621
AlwI GGATC 3 cut(s) 268, 510, 523
AlwNI CAGNNNCTG 1 cut(s) 455
Ama87I CYCGRG 1 cut(s) 225
AoxI GGCC 7 cut(s) 6, 66, 428, 571, 907, 1092, 1217
ApaLI GTGCAC 1 cut(s) 621
ApeKI GCWGC 3 cut(s) 881, 884, 887
ApoI RAATTY 1 cut(s) 752
ArsI GACNNNNNNTTYG 4 cut(s) 125, 157, 317, 349
AspLEI GCGC 5 cut(s) 20, 40, 353, 410, 589
AspS9I GGNCC 3 cut(s) 6, 458, 1051
AsuC2I CCSGG 3 cut(s) 226, 227, 545
AsuHPI GGTGA 3 cut(s) 107, 761, 969
AvaI CYCGRG 1 cut(s) 225
AvaII GGWCC 2 cut(s) 458, 1051
BaeGI GKGCMC 1 cut(s) 625
BalI TGGCCA 2 cut(s) 68, 1219
BamHI GGATCC 1 cut(s) 515
BanII GRGCYC 1 cut(s) 1154
BauI CACGAG 1 cut(s) 1065
BbsI GAAGAC 1 cut(s) 1112
Bbv12I GWGCWC 2 cut(s) 625, 1154
BbvI GCAGC 3 cut(s) 893, 896, 899
BccI CCATC 2 cut(s) 560, 864
BciT130I CCWGG 6 cut(s) 432, 725, 822, 906, 936, 1197
BcnI CCSGG 3 cut(s) 226, 227, 545
BcoDI GTCTC 2 cut(s) 103, 492
BfaI CTAG 1 cut(s) 1016
BfmI CTRYAG 2 cut(s) 360, 831
BfoI RGCGCY 1 cut(s) 354
BfuAI ACCTGC 1 cut(s) 413
BisI GCNGC 3 cut(s) 882, 885, 888
BlsI GCNGC 3 cut(s) 883, 886, 889
BmcAI AGTACT 1 cut(s) 283
Bme1390I CCNGG 9 cut(s) 226, 227, 432, 545, 725, 822, 906, 936, 1197
Bme18I GGWCC 2 cut(s) 458, 1051
BmeT110I CYCGRG 1 cut(s) 225
BmgBI CACGTC 1 cut(s) 314
BmgT120I GGNCC 3 cut(s) 6, 458, 1051
BmiI GGNNCC 1 cut(s) 517
BmrFI CCNGG 9 cut(s) 226, 227, 432, 545, 725, 822, 906, 936, 1197
BmrI ACTGGG 3 cut(s) 285, 589, 1151
BmsI GCATC 3 cut(s) 574, 696, 1214
BmuI ACTGGG 3 cut(s) 285, 589, 1151
BpiI GAAGAC 1 cut(s) 1112
BplI GAGNNNNNCTC 2 cut(s) 487, 519
Bpu10I CCTNAGC 1 cut(s) 590
BpuMI CCSGG 3 cut(s) 226, 227, 545
BsaBI GATNNNNATC 1 cut(s) 1204
BsaHI GRCGYC 1 cut(s) 655
BsaJI CCNNGG 5 cut(s) 63, 225, 474, 910, 961
Bsc4I CCNNNNNNNGG 6 cut(s) 580, 730, 782, 845, 911, 1184
Bse1I ACTGG 4 cut(s) 280, 289, 584, 1157
Bse8I GATNNNNATC 1 cut(s) 1204
BseBI CCWGG 6 cut(s) 432, 725, 822, 906, 936, 1197
BseDI CCNNGG 5 cut(s) 63, 225, 474, 910, 961
BseGI GGATG 2 cut(s) 589, 679
BseJI GATNNNNATC 1 cut(s) 1204
BseLI CCNNNNNNNGG 6 cut(s) 580, 730, 782, 845, 911, 1184
BseMII CTCAG 3 cut(s) 114, 399, 439
BseNI ACTGG 4 cut(s) 280, 289, 584, 1157
BseRI GAGGAG 2 cut(s) 494, 1162
BseSI GKGCMC 1 cut(s) 625
BseXI GCAGC 3 cut(s) 893, 896, 899
BseYI CCCAGC 1 cut(s) 305
BsgI GTGCAG 3 cut(s) 807, 885, 1163
BshFI GGCC 7 cut(s) 8, 68, 430, 573, 909, 1094, 1219
BsiHKAI GWGCWC 2 cut(s) 625, 1154
BsiHKCI CYCGRG 1 cut(s) 225
BsiSI CCGG 3 cut(s) 81, 226, 544
BslFI GGGAC 3 cut(s) 89, 324, 1021
BslI CCNNNNNNNGG 6 cut(s) 580, 730, 782, 845, 911, 1184
BsmAI GTCTC 2 cut(s) 103, 492
BsmBI CGTCTC 1 cut(s) 103
BsmFI GGGAC 3 cut(s) 89, 324, 1021
BsmI GAATGC 2 cut(s) 178, 253
BsnI GGCC 7 cut(s) 8, 68, 430, 573, 909, 1094, 1219
BsoBI CYCGRG 1 cut(s) 225
Bsp1286I GDGCHC 2 cut(s) 625, 1154
Bsp143I GATC 2 cut(s) 260, 515
Bsp19I CCATGG 1 cut(s) 63
BspACI CCGC 1 cut(s) 843
BspANI GGCC 7 cut(s) 8, 68, 430, 573, 909, 1094, 1219
BspCNI CTCAG 3 cut(s) 113, 398, 438
BspHI TCATGA 1 cut(s) 295
BspLI GGNNCC 1 cut(s) 517
BspMAI CTGCAG 1 cut(s) 364
BspMI ACCTGC 1 cut(s) 413
BspPI GGATC 3 cut(s) 268, 510, 523
BsrI ACTGG 4 cut(s) 280, 289, 584, 1157
BssECI CCNNGG 5 cut(s) 63, 225, 474, 910, 961
BssMI GATC 2 cut(s) 260, 515
BssNI GRCGYC 1 cut(s) 655
BssSI CACGAG 1 cut(s) 1065
BssT1I CCWWGG 4 cut(s) 63, 474, 910, 961
Bst2BI CACGAG 1 cut(s) 1065
Bst2UI CCWGG 6 cut(s) 432, 725, 822, 906, 936, 1197
Bst4CI ACNGT 2 cut(s) 378, 1087
Bst6I CTCTTC 1 cut(s) 856
BstACI GRCGYC 1 cut(s) 655
BstAPI GCANNNNNTGC 1 cut(s) 35
BstC8I GCNNGC 5 cut(s) 214, 597, 687, 802, 1186
BstDEI CTNAG 4 cut(s) 100, 385, 425, 590
BstDSI CCRYGG 1 cut(s) 63
BstF5I GGATG 2 cut(s) 589, 679
BstH2I RGCGCY 1 cut(s) 354
BstHHI GCGC 5 cut(s) 20, 40, 353, 410, 589
BstKTI GATC 2 cut(s) 263, 518
BstMAI GTCTC 2 cut(s) 103, 492
BstMBI GATC 2 cut(s) 260, 515
BstMWI GCNNNNNNNGC 5 cut(s) 26, 35, 359, 416, 887
BstNI CCWGG 6 cut(s) 432, 725, 822, 906, 936, 1197
BstNSI RCATGY 2 cut(s) 216, 599
BstSCI CCNGG 9 cut(s) 224, 225, 430, 543, 723, 820, 904, 934, 1195
BstSFI CTRYAG 2 cut(s) 360, 831
BstSLI GKGCMC 1 cut(s) 625
BstV1I GCAGC 3 cut(s) 893, 896, 899
BstV2I GAAGAC 1 cut(s) 1112
BstX2I RGATCY 2 cut(s) 260, 515
BstYI RGATCY 2 cut(s) 260, 515
BsuRI GGCC 7 cut(s) 8, 68, 430, 573, 909, 1094, 1219
BtgI CCRYGG 1 cut(s) 63
BtrI CACGTC 1 cut(s) 314
BtsCI GGATG 2 cut(s) 589, 679
BtsI GCAGTG 2 cut(s) 171, 201
BtsIMutI CAGTG 2 cut(s) 171, 201
BveI ACCTGC 1 cut(s) 413
Cac8I GCNNGC 5 cut(s) 214, 597, 687, 802, 1186
CaiI CAGNNNCTG 1 cut(s) 455
CciI TCATGA 1 cut(s) 295
CfoI GCGC 5 cut(s) 20, 40, 353, 410, 589
Cfr13I GGNCC 3 cut(s) 6, 458, 1051
Cfr9I CCCGGG 1 cut(s) 225
CseI GACGC 2 cut(s) 644, 1106
Csp6I GTAC 3 cut(s) 59, 282, 1088
CviQI GTAC 3 cut(s) 59, 282, 1088
DdeI CTNAG 4 cut(s) 100, 385, 425, 590
DpnI GATC 2 cut(s) 262, 517
DpnII GATC 2 cut(s) 260, 515
EaeI YGGCCR 2 cut(s) 66, 1217
Eam1104I CTCTTC 1 cut(s) 856
EarI CTCTTC 1 cut(s) 856
Ecl136II GAGCTC 1 cut(s) 1152
Eco130I CCWWGG 4 cut(s) 63, 474, 910, 961
Eco24I GRGCYC 1 cut(s) 1154
Eco47I GGWCC 2 cut(s) 458, 1051
Eco53kI GAGCTC 1 cut(s) 1152
Eco57I CTGAAG 1 cut(s) 582
Eco88I CYCGRG 1 cut(s) 225
EcoICRI GAGCTC 1 cut(s) 1152
EcoRII CCWGG 6 cut(s) 430, 723, 820, 904, 934, 1195
EcoT14I CCWWGG 4 cut(s) 63, 474, 910, 961
EcoT22I ATGCAT 1 cut(s) 1207
EcoT38I GRGCYC 1 cut(s) 1154
ErhI CCWWGG 4 cut(s) 63, 474, 910, 961
Esp3I CGTCTC 1 cut(s) 103
FalI AAGNNNNNCTT 2 cut(s) 540, 572
FaqI GGGAC 3 cut(s) 89, 324, 1021
Fnu4HI GCNGC 3 cut(s) 882, 885, 888
FokI GGATG 2 cut(s) 596, 686
FriOI GRGCYC 1 cut(s) 1154
Fsp4HI GCNGC 3 cut(s) 882, 885, 888
FspBI CTAG 1 cut(s) 1016
GlaI GCGC 5 cut(s) 19, 39, 352, 409, 588
GluI GCNGC 3 cut(s) 882, 885, 888
GsaI CCCAGC 1 cut(s) 309
HaeII RGCGCY 1 cut(s) 354
HaeIII GGCC 7 cut(s) 8, 68, 430, 573, 909, 1094, 1219
HapII CCGG 3 cut(s) 81, 226, 544
HgaI GACGC 2 cut(s) 644, 1106
HhaI GCGC 5 cut(s) 20, 40, 353, 410, 589
Hin1I GRCGYC 1 cut(s) 655
Hin6I GCGC 5 cut(s) 18, 38, 351, 408, 587
HinP1I GCGC 5 cut(s) 18, 38, 351, 408, 587
HindIII AAGCTT 2 cut(s) 237, 330
HinfI GANTC 5 cut(s) 170, 356, 500, 712, 920
HpaII CCGG 3 cut(s) 81, 226, 544
HphI GGTGA 3 cut(s) 107, 761, 969
Hpy166II GTNNAC 1 cut(s) 623
Hpy188I TCNGA 6 cut(s) 50, 129, 184, 191, 660, 1210
Hpy188III TCNNGA 6 cut(s) 149, 244, 296, 323, 387, 1112
Hpy8I GTNNAC 1 cut(s) 623
HpyAV CCTTC 2 cut(s) 100, 1070
HpyCH4III ACNGT 2 cut(s) 378, 1087
HpyCH4IV ACGT 1 cut(s) 313
HpyF10VI GCNNNNNNNGC 5 cut(s) 26, 35, 359, 416, 887
HpyF3I CTNAG 4 cut(s) 100, 385, 425, 590
HpySE526I ACGT 1 cut(s) 313
Hsp92I GRCGYC 1 cut(s) 655
HspAI GCGC 5 cut(s) 18, 38, 351, 408, 587
Kzo9I GATC 2 cut(s) 260, 515
LmnI GCTCC 2 cut(s) 1149, 1157
Lsp1109I GCAGC 3 cut(s) 893, 896, 899
LweI GCATC 3 cut(s) 574, 696, 1214
MaeI CTAG 1 cut(s) 1016
MaeII ACGT 1 cut(s) 313
MaeIII GTNAC 1 cut(s) 938
MalI GATC 2 cut(s) 262, 517
MboI GATC 2 cut(s) 260, 515
MboII GAAGA 3 cut(s) 575, 873, 1112
MfeI CAATTG 2 cut(s) 690, 1179
MflI RGATCY 2 cut(s) 260, 515
MhlI GDGCHC 2 cut(s) 625, 1154
MlsI TGGCCA 2 cut(s) 68, 1219
MluCI AATT 9 cut(s) 202, 255, 342, 690, 699, 739, 752, 795, 1179
MluNI TGGCCA 2 cut(s) 68, 1219
MlyI GAGTC 1 cut(s) 494
Mox20I TGGCCA 2 cut(s) 68, 1219
Mph1103I ATGCAT 1 cut(s) 1207
MscI TGGCCA 2 cut(s) 68, 1219
MseI TTAA 5 cut(s) 234, 665, 702, 794, 1225
Msp20I TGGCCA 2 cut(s) 68, 1219
MspI CCGG 3 cut(s) 81, 226, 544
MspR9I CCNGG 9 cut(s) 226, 227, 432, 545, 725, 822, 906, 936, 1197
MunI CAATTG 2 cut(s) 690, 1179
Mva1269I GAATGC 2 cut(s) 178, 253
MvaI CCWGG 6 cut(s) 432, 725, 822, 906, 936, 1197
MwoI GCNNNNNNNGC 5 cut(s) 26, 35, 359, 416, 887
NciI CCSGG 3 cut(s) 226, 227, 545
NcoI CCATGG 1 cut(s) 63
NdeII GATC 2 cut(s) 260, 515
NlaIV GGNNCC 1 cut(s) 517
NmuCI GTSAC 1 cut(s) 938
NsiI ATGCAT 1 cut(s) 1207
NspI RCATGY 2 cut(s) 216, 599
PaeI GCATGC 2 cut(s) 216, 599
PagI TCATGA 1 cut(s) 295
PctI GAATGC 2 cut(s) 178, 253
PfeI GAWTC 4 cut(s) 170, 356, 712, 920
PflMI CCANNNNNTGG 2 cut(s) 845, 1184
PfoI TCCNGGA 1 cut(s) 1195
PkrI GCNGC 3 cut(s) 883, 886, 889
PleI GAGTC 1 cut(s) 494
PpsI GAGTC 1 cut(s) 494
Psp124BI GAGCTC 1 cut(s) 1154
Psp6I CCWGG 6 cut(s) 430, 723, 820, 904, 934, 1195
PspFI CCCAGC 1 cut(s) 305
PspGI CCWGG 6 cut(s) 430, 723, 820, 904, 934, 1195
PspN4I GGNNCC 1 cut(s) 517
PspPI GGNCC 3 cut(s) 6, 458, 1051
PstI CTGCAG 1 cut(s) 364
PstNI CAGNNNCTG 1 cut(s) 455
PsuI RGATCY 2 cut(s) 260, 515
RsaI GTAC 3 cut(s) 60, 283, 1089
RsaNI GTAC 3 cut(s) 59, 282, 1088
SacI GAGCTC 1 cut(s) 1154
SaqAI TTAA 5 cut(s) 234, 665, 702, 794, 1225
SatI GCNGC 3 cut(s) 882, 885, 888
Sau3AI GATC 2 cut(s) 260, 515
Sau96I GGNCC 3 cut(s) 6, 458, 1051
ScaI AGTACT 1 cut(s) 283
SchI GAGTC 1 cut(s) 494
ScrFI CCNGG 9 cut(s) 226, 227, 432, 545, 725, 822, 906, 936, 1197
SduI GDGCHC 2 cut(s) 625, 1154
SfaNI GCATC 3 cut(s) 574, 696, 1214
SfcI CTRYAG 2 cut(s) 360, 831
SinI GGWCC 2 cut(s) 458, 1051
SmaI CCCGGG 1 cut(s) 227
SphI GCATGC 2 cut(s) 216, 599
Sse9I AATT 9 cut(s) 202, 255, 342, 690, 699, 739, 752, 795, 1179
SsiI CCGC 1 cut(s) 843
SspMI CTAG 1 cut(s) 1016
SstI GAGCTC 1 cut(s) 1154
StyD4I CCNGG 9 cut(s) 224, 225, 430, 543, 723, 820, 904, 934, 1195
StyI CCWWGG 4 cut(s) 63, 474, 910, 961
TaaI ACNGT 2 cut(s) 378, 1087
TaiI ACGT 1 cut(s) 316
TasI AATT 9 cut(s) 202, 255, 342, 690, 699, 739, 752, 795, 1179
TatI WGTACW 2 cut(s) 281, 1087
TfiI GAWTC 4 cut(s) 170, 356, 712, 920
Tru1I TTAA 5 cut(s) 234, 665, 702, 794, 1225
Tru9I TTAA 5 cut(s) 234, 665, 702, 794, 1225
TscAI CASTG 2 cut(s) 171, 201
TseFI GTSAC 1 cut(s) 938
TseI GCWGC 3 cut(s) 881, 884, 887
Tsp45I GTSAC 1 cut(s) 938
TspDTI ATGAA 4 cut(s) 315, 627, 639, 1155
TspGWI ACGGA 1 cut(s) 85
TspMI CCCGGG 1 cut(s) 225
TspRI CASTG 2 cut(s) 171, 201
Van91I CCANNNNNTGG 2 cut(s) 845, 1184
VneI GTGCAC 1 cut(s) 621
VpaK11BI GGWCC 2 cut(s) 458, 1051
XapI RAATTY 1 cut(s) 752
XceI RCATGY 2 cut(s) 216, 599
XmaI CCCGGG 1 cut(s) 225
XspI CTAG 1 cut(s) 1016
ZrmI AGTACT 1 cut(s) 283
Zsp2I ATGCAT 1 cut(s) 1207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.