Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0006487

Physical Location & Seq

Genomic Coordinates Forward (+)

36537181 .. 36547460

Gene Structure 10280 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1285800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3762 bp

ATGGTAGAAACCAGGCGCAGCTCCTCTTCCAAACGCGCCCTATCGGCCTCCCCTCCTCCCAACCCCAAACGCTCAAAGGCGTCTGATGCTTCGTCGTCGAACAACGGAGTTAGGAGTGGGCCGCCGGCGGAGCCTTTGGGTCCGATTAAGGAATCTGGGTCCCAATCTCCGGAACTTGAGCTCCGACCCTCTGATCCGCCGACCACTGATTCGTTGAAGGCAAGTAACGGCTCTGATGCCACCGCGCTGGAGAGATCCCCCGATGCCATACCGGAAGGCGAGGCCTTGGTTTCGCCGCAGCCTTTGGGGGAAACTGCAGTCCGTGCGGGTTTGAAGCGGGCTAAGAAGCTTCCAAAGAAGACTGCAAAGTTGAATTCGAAATCTGCTTGGGGAATGCTTATTTCGCAGTGCTCAAAGAACCCGCACCTGTTCATTTGTGATACTGTTTTCACTGTTGGTCAAAGTCGTGAGTGCCATTTATGTATTAAAGACCCATCCATTAGTACTACTTTGTGTAAACTGAAACATGTTAAGCGTGAAGGTTTGTCCACTGCAGAACTAGAAATTATAGGCGGTAACGGTGATGTTCAGGTGAATGAGAAGACTTATCAAAAGGATACGAAGGTAATTCTTAATGGAGGCGATGAGGTTGTATTCAGCTCATCTGGAAGACATGCATATATTTTCCAGCAGCTGACAAATGACAATAATATAGCTGCTCAGGGCATTCCTTCAATTAGCATTATAGAAACCCGGAATGCTCCAGTTAATGGGATGCATATGGAGGCAAGATCAGGGGACCCCTCTGCTGTTGATGGGGCATCAATATTAGCGTCAATGTCAAATGTGCCAAATGACTTGTCACTACTTCCAGAACCTGCTAAAGCTGGTGACGAGCTGCAACAAGATGCAGAGATGACTTCTCTTCCTTCTGCATGTCGAGGTTCAGATGACCATACTCTAGACATTGTGATGAAGGAGAGTACTAACATTAATGATCAGGTTTCAGGTGATAAAGATATTGTTCAGTACCCTGACATTGCGAAAGAAAACCCCAATCTTGATAGCGTTGCATTGGATATGGATACTGAAACCAGGAAGGTCTCTGGGGCAACTCATCAACTAAGGCCACTCCTCCGTATGTTTGGCGGTTCTTCTAGTACCAATTTTGACTTAACTGGCAGCATTTCTAAAATGTTTGACGAGCAAAGGGAAATCAGAGAGCTTCTCCACAATCTTGACCCTCCAATGTTGATATCAACTAGGCGACAAGCATTTAAAGAAAAACTACAACAAGGAATTCTAAGTCCTGATGATATTGATGTCTCCTTTGAAAGTTTCCCATATTACCTAAGTGAAACAACAAAGAAGGTTTTGATTGCATCCACCCACCCAAATTTGAAATGTAGTAAGTTTGGAAAGTACTTTTCATCACTACCTACTGGGAGCCCGCGCATATTATTATCTGGTCCAGCAGGTTCTGAGATATATCAGGAGACCTTGGCAAAGGCACTTACGAAACATTTTGGTGCTAGATTACTAATTGTAGATTCTCTTCTCCTGCCTGGTGTTCAGGCACCACCGCCCAAGGATTCTGATTCTGTTAAAGAAGTACCAAGGCCTGAAAGAGTTTCCATTTTTGCGAAACGAGCAGCTCATGCTGCTGGGTTCAAGCACAAGAAACCAACCTCTAGTGTTGAGGCTGAAATTACTGGTGGATCCACTGTGAGTTCTCAGGCACTGCCAAAGCAGGAGACTTCTACTGCATCATCCAGAGGCATTACATTTAAACAAGGTGACAAGGTCAAATTTGTGGGTGCCATATCTTCTGGTCCTCTGCAGTTGCAGAGTTGTCCTCTTCTAAGGGGACCATCATATGGTTGTCGAGGCAAAGTTGTTCTTGCTTTTGAAGAAAATGAGGCTGCAAAAATTGGGGTTAGATTTGATAAATCAATACCAGATGGCAATGATCTTGGTGGGCTATGTGAAGAAGATCATGGCTTTTTTTGCTCTGCTAGTCATTTACTTCCCTTGGATGTTTCTGGAGGTGATGATATTGACAAGCTCGCTATTGGTGAACTTCTTGAGGTTGCATCCCATGAGAGTAAAAGTCTTCCTTTGATAGTGTTTTTGAAAGATATAGAAAAGGTTATGGCAGGTAACCCAGATGCATATAGTGTCTTGAAGAGTAAGCTTGAAAACTTGCCAGAGAATGTTGTTGTGATCGGCTCTCATACCCAGTTGGACACTCGCAAGGAGAAGTCCCATCCTGGTAGCCTTCTGTTCACAAAATTTGGCTTCAACCAAACGGCTTTGCTTGATCTTGCTTTTCCGGATAACCTTGGTGGTAGGCTGCATGATAGGAGCAAGGAAACTCCTAAAACTATGAAGCAACTCTCTCGGATTTTCCCCAACAAAGTGACCATACAGCTGCCCCAGGATGAAGCTTTACTATCGGACTGGAAGCAGCAGTTGGAGCGTGATGGTGAAACTTTGAAAGCCCAGTCAAATATCGTTACCATTCGCTCAGTTTTTAACCGAGTTGCTGTGGATTGCCCTGACCTTGAAAGTCTCTGCATTAATGATCTAGCGCTTACAACTGAAAGTGTTGAGAAAGTAGCAGGCTGGGCTATTAGTCACCATCTTATGCATTGTTCGGACGCTTTAGTTAAAGATGACAAACTTGTTATTTCTACTGAAAGCCTTAAGGATGGACTAAACATTCTACACGGCATTCAAAATGAAAACAAGAGCATGAAGAAATCACTTAAGGATGTGGTTACCGGAAACGAATTTGAGAAAAAACTTCTTGTTGATGTTATTCCACCAAGCGATATTGGGGTTAGTTTTGATGACATCGGGGCCTTGGAAAATGTGAAGGACACCCTAAAGGAGTTGGTGATGCTTCCTCTTCAGAGGCCTGAATTGTTTAGCAAAGGACAATTGACTAAGCCTTGCAAAGGAATCTTGCTATTTGGTCCTCCGGGTACTGGAAAAACTATGCTTGCAAAGGCTGTTGCAACTGAAGCTGGTGCAAACTTTATTAACATTTCAATGTCAAGTATTACTTCAAAGTGGTTTGGGGAAGGAGAGAAGTACGTTAAAGCTGTGTTCTCCTTAGCTAGTAAAATTGCCCCTAGCGTCATTTTTGTTGATGAGGTTGACAGCATGTTAGGAAGACGTGAAAATCCTGGGGAGCATGAAGCTATGCGTAAAATGAAAAATGAGTTCATGGTGAACTGGGATGGTTTGCGTACAAAGGATAAAGAACGTGTATTGGTACTTGCTGCTACTAATAGGCCTTTTGACCTTGATGAGGCCGTTATTAGGCGGCTTCCAAGGAGATTGATGGTGAACTTGCCAGATGCCCAGAACAGAGAGAAAATACTGAGAGTTATATTAGCCAAGGAAGATTTGGAAGCTGACGTTGATTTGGAAGCAGTTGCAAATCTTACGGATGGGTATTCAGGAAGTGACTTAAAGAATCTGTGTGTTGCTGCAGCTCACCTCCCCATCAGGGAAATTTTAGAGAAAGAGAAAAAGGAGAGAAGTTTAGCTTTGGCGGAGAACAGACCTGTACCCGAGTTGTACTGTAGTACCGACATTCGTCCTTTGAAGATGGAGGACTTTAAACATGCGCATGAGCAGGTATGTGCAAGTGTGTCATCAGAGTCGACAAATATGAACGAGCTCCTCCAATGGAATGACCTATATGGCGAAGGCGGATCAAGAAAGAAGACGTCTCTCAGTTATTTTATGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002090 GO:0002092 GO:0003008 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0007610 GO:0007611 GO:0007612 GO:0007613 GO:0008150 GO:0009893 GO:0010604 GO:0010646 GO:0010648 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019222 GO:0023051 GO:0023057 GO:0030100 GO:0031323 GO:0031325 GO:0032501 GO:0032879 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044456 GO:0044459 GO:0044464 GO:0045202 GO:0045211 GO:0045807 GO:0048259 GO:0048260 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050804 GO:0050805 GO:0050877 GO:0050890 GO:0051049 GO:0051050 GO:0051128 GO:0051130 GO:0051966 GO:0051967 GO:0060255 GO:0060627 GO:0065007 GO:0071944 GO:0097060 GO:0098590 GO:0098794 GO:0099177

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA FHA

Protein Analysis

1254

Amino Acids

136.61

Weight (kDa)

6.09

Isoelectric Point (pI)

41.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF7751	PF24933	850 - 910	5.4e-25	Domain of unknown function (DUF7751)
AAA	PF00004	989 - 1119	3.7e-38	ATPase family associated with various cellular activities (AAA)
AAA_lid_3	PF17862	1142 - 1178	3e-13	AAA+ lid domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006487)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G02890 AT1G02890 AT4G02480
fragaria_vesca	FvH4_2g25490 FvH4_2g25490 FvH4_2g25490
malus_domestica	MD03G1285800.v1.1 MD11G1305200.v1.1 MD11G1305300.v1.1 MD11G1305400.v1.1
prunus_persica	Prupe.8G259000_v2.0.a1 Prupe.8G259000_v2.0.a1 Prupe.8G259000_v2.0.a1 Prupe.8G259000_v2.0.a1
pyrus_communis	pycom03g22650 pycom11g26700 pycom11g26720
rosa_chinensis	RchiOBHm_Chr6g0293701
rosa_laevigata	RLG00000011931
rosa_roxburghii	Rroxscaffold_7G00173960
rosa_rugosa	Rorug06G0237500
rosa_samantha	Rh6AG350200 Rh6BG357700 Rh6CG364500 Rh6DG351400
rosa_wichuraiana	Rw6G030490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	3743
Acc16I	TGCGCA	1 cut(s)	3639
Acc36I	ACCTGC	4 cut(s)	886, 1466, 2147, 3637
AccB1I	GGYRCC	2 cut(s)	1576, 1817
AccB7I	CCANNNNNTGG	2 cut(s)	770, 1877
AccI	GTMKAC	1 cut(s)	3674
AccII	CGCG	3 cut(s)	36, 245, 1453
AccIII	TCCGGA	2 cut(s)	169, 2332
AclWI	GGATC	5 cut(s)	188, 249, 1713, 1726, 3733
AcsI	RAATTY	7 cut(s)	373, 1299, 1396, 1808, 2291, 2792, 3522
AcuI	CTGAAG	2 cut(s)	2897, 3045
AcyI	GRCGYC	2 cut(s)	80, 3740
AfeI	AGCGCT	1 cut(s)	2592
AfiI	CCNNNNNNNGG	8 cut(s)	169, 770, 1877, 2960, 2990, 3316, 3327, 3517
AflII	CTTAAG	2 cut(s)	2705, 2768
AflIII	ACRYGT	2 cut(s)	526, 3271
AjiI	CACGTC	1 cut(s)	3182
AjnI	CCWGG	6 cut(s)	11, 1094, 1564, 2269, 2436, 3190
AjuI	GAANNNNNNNTTGG	2 cut(s)	2456, 2488
AloI	GAACNNNNNNTCC	2 cut(s)	165, 197
Alw21I	GWGCWC	3 cut(s)	183, 413, 3693
Alw26I	GTCTC	6 cut(s)	1108, 1330, 1490, 1748, 2576, 3747
AlwI	GGATC	5 cut(s)	188, 249, 1713, 1726, 3733
AlwNI	CAGNNNCTG	4 cut(s)	694, 878, 1481, 1741
Ama87I	CYCGRG	1 cut(s)	3581
Aor13HI	TCCGGA	2 cut(s)	169, 2332
Aor51HI	AGCGCT	1 cut(s)	2592
AoxI	GGCC	9 cut(s)	45, 119, 282, 1127, 1619, 2862, 2918, 3299, 3318
ApoI	RAATTY	7 cut(s)	373, 1299, 1396, 1808, 2291, 2792, 3522
ArsI	GACNNNNNNTTYG	4 cut(s)	193, 225, 845, 877
AseI	ATTAAT	2 cut(s)	993, 2580
AspLEI	GCGC	6 cut(s)	18, 38, 247, 1455, 2593, 3640
AspS9I	GGNCC	9 cut(s)	119, 140, 159, 799, 1469, 1832, 1868, 2862, 2978
AsuC2I	CCSGG	2 cut(s)	754, 2985
AsuII	TTCGAA	1 cut(s)	377
AvaI	CYCGRG	1 cut(s)	3581
AvaII	GGWCC	7 cut(s)	140, 159, 799, 1469, 1832, 1868, 2978
BaeI	ACNNNNGTAYC	4 cut(s)	609, 642, 3580, 3613
BamHI	GGATCC	1 cut(s)	1718
BanI	GGYRCC	2 cut(s)	1576, 1817
BanII	GRGCYC	3 cut(s)	183, 1451, 3693
BarI	GAAGNNNNNNTAC	2 cut(s)	1539, 1571
BbsI	GAAGAC	6 cut(s)	365, 608, 676, 2105, 3184, 3743
Bbv12I	GWGCWC	3 cut(s)	183, 413, 3693
BceAI	ACGGC	4 cut(s)	244, 2325, 2746, 3305
BcgI	CGANNNNNNTGC	2 cut(s)	2381, 2415
BciT130I	CCWGG	6 cut(s)	13, 1096, 1566, 2271, 2438, 3192
BciVI	GTATCC	2 cut(s)	610, 1078
BclI	TGATCA	1 cut(s)	997
BcnI	CCSGG	2 cut(s)	754, 2985
BcoDI	GTCTC	6 cut(s)	1108, 1330, 1490, 1748, 2576, 3747
BfmI	CTRYAG	5 cut(s)	315, 552, 1838, 3498, 3592
BfoI	RGCGCY	1 cut(s)	2594
BfrI	CTTAAG	2 cut(s)	2705, 2768
BfuAI	ACCTGC	4 cut(s)	886, 1466, 2147, 3637
BfuI	GTATCC	2 cut(s)	610, 1078
BglI	GCCNNNNNGGC	1 cut(s)	44
BmcAI	AGTACT	3 cut(s)	505, 985, 1424
Bme1390I	CCNGG	8 cut(s)	13, 754, 1096, 1566, 2271, 2438, 2985, 3192
Bme18I	GGWCC	7 cut(s)	140, 159, 799, 1469, 1832, 1868, 2978
BmeT110I	CYCGRG	1 cut(s)	3581
BmgBI	CACGTC	1 cut(s)	3182
BmgT120I	GGNCC	9 cut(s)	119, 140, 159, 799, 1469, 1832, 1868, 2862, 2978
BmrFI	CCNGG	8 cut(s)	13, 754, 1096, 1566, 2271, 2438, 2985, 3192
BmrI	ACTGGG	4 cut(s)	1452, 2233, 2497, 3250
BmuI	ACTGGG	4 cut(s)	1452, 2233, 2497, 3250
BoxI	GACNNNNGTC	1 cut(s)	3606
BpiI	GAAGAC	6 cut(s)	365, 608, 676, 2105, 3184, 3743
BplI	GAGNNNNNCTC	4 cut(s)	1212, 1244, 1840, 1872
BpmI	CTGGAG	3 cut(s)	269, 747, 2064
Bpu10I	CCTNAGC	2 cut(s)	720, 3118
Bpu14I	TTCGAA	1 cut(s)	377
BpuEI	CTTGAG	2 cut(s)	197, 2105
BpuMI	CCSGG	2 cut(s)	754, 2985
BsaHI	GRCGYC	2 cut(s)	80, 3740
BsaI	GGTCTC	2 cut(s)	1108, 1490
BsaWI	WCCGGW	4 cut(s)	169, 271, 2332, 2783
BsaXI	ACNNNNNCTCC	2 cut(s)	165, 195
Bsc4I	CCNNNNNNNGG	8 cut(s)	169, 770, 1877, 2960, 2990, 3316, 3327, 3517
Bse118I	RCCGGY	1 cut(s)	124
Bse1I	ACTGG	9 cut(s)	764, 1183, 1447, 1717, 2239, 2465, 2503, 2995, 3245
Bse3DI	GCAATG	2 cut(s)	1038, 1972
BseAI	TCCGGA	2 cut(s)	169, 2332
BseBI	CCWGG	6 cut(s)	13, 1096, 1566, 2271, 2438, 3192
BseLI	CCNNNNNNNGG	8 cut(s)	169, 770, 1877, 2960, 2990, 3316, 3327, 3517
BseMI	GCAATG	2 cut(s)	1038, 1972
BseMII	CTCAG	6 cut(s)	734, 1473, 1748, 2541, 3380, 3760
BseNI	ACTGG	9 cut(s)	764, 1183, 1447, 1717, 2239, 2465, 2503, 2995, 3245
BseRI	GAGGAG	4 cut(s)	13, 45, 1124, 3683
BseYI	CCCAGC	2 cut(s)	1664, 2625
Bsh1236I	CGCG	3 cut(s)	36, 245, 1453
BshFI	GGCC	9 cut(s)	47, 121, 284, 1129, 1621, 2864, 2920, 3301, 3320
BshNI	GGYRCC	2 cut(s)	1576, 1817
BsiHKAI	GWGCWC	3 cut(s)	183, 413, 3693
BsiHKCI	CYCGRG	1 cut(s)	3581
BsiSI	CCGG	7 cut(s)	125, 170, 272, 754, 2333, 2784, 2984
BslFI	GGGAC	4 cut(s)	145, 812, 1881, 2248
BslI	CCNNNNNNNGG	8 cut(s)	169, 770, 1877, 2960, 2990, 3316, 3327, 3517
BsmAI	GTCTC	6 cut(s)	1108, 1330, 1490, 1748, 2576, 3747
BsmBI	CGTCTC	1 cut(s)	3747
BsmFI	GGGAC	4 cut(s)	145, 812, 1881, 2248
BsmI	GAATGC	4 cut(s)	399, 726, 763, 2733
BsnI	GGCC	9 cut(s)	47, 121, 284, 1129, 1621, 2864, 2920, 3301, 3320
Bso31I	GGTCTC	2 cut(s)	1108, 1490
BsoBI	CYCGRG	1 cut(s)	3581
Bsp119I	TTCGAA	1 cut(s)	377
Bsp1286I	GDGCHC	4 cut(s)	183, 413, 1451, 3693
Bsp13I	TCCGGA	2 cut(s)	169, 2332
BspANI	GGCC	9 cut(s)	47, 121, 284, 1129, 1621, 2864, 2920, 3301, 3320
BspCNI	CTCAG	6 cut(s)	733, 1474, 1747, 2540, 3381, 3759
BspEI	TCCGGA	2 cut(s)	169, 2332
BspFNI	CGCG	3 cut(s)	36, 245, 1453
BspMAI	CTGCAG	4 cut(s)	319, 556, 1842, 3502
BspMI	ACCTGC	4 cut(s)	886, 1466, 2147, 3637
BspPI	GGATC	5 cut(s)	188, 249, 1713, 1726, 3733
BspT104I	TTCGAA	1 cut(s)	377
BspT107I	GGYRCC	2 cut(s)	1576, 1817
BspTI	CTTAAG	2 cut(s)	2705, 2768
BspTNI	GGTCTC	2 cut(s)	1108, 1490
BsrDI	GCAATG	2 cut(s)	1038, 1972
BsrFI	RCCGGY	1 cut(s)	124
BsrI	ACTGG	9 cut(s)	764, 1183, 1447, 1717, 2239, 2465, 2503, 2995, 3245
BssAI	RCCGGY	1 cut(s)	124
BssNI	GRCGYC	2 cut(s)	80, 3740
BssT1I	CCWWGG	9 cut(s)	285, 1500, 1587, 1616, 2031, 2341, 2865, 3338, 3405
Bst2UI	CCWGG	6 cut(s)	13, 1096, 1566, 2271, 2438, 3192
Bst4CI	ACNGT	5 cut(s)	445, 454, 581, 1726, 3593
Bst6I	CTCTTC	6 cut(s)	31, 930, 1560, 1863, 2180, 2916
BstACI	GRCGYC	2 cut(s)	80, 3740
BstAFI	CTTAAG	2 cut(s)	2705, 2768
BstAPI	GCANNNNNTGC	2 cut(s)	323, 1658
BstBI	TTCGAA	1 cut(s)	377
BstC8I	GCNNGC	6 cut(s)	126, 339, 1451, 2067, 2623, 3006
BstEII	GGTNACC	2 cut(s)	2159, 2779
BstENI	CCTNNNNNAGG	2 cut(s)	2958, 3314
BstFNI	CGCG	3 cut(s)	36, 245, 1453
BstH2I	RGCGCY	1 cut(s)	2594
BstHHI	GCGC	6 cut(s)	18, 38, 247, 1455, 2593, 3640
BstMAI	GTCTC	6 cut(s)	1108, 1330, 1490, 1748, 2576, 3747
BstNI	CCWGG	6 cut(s)	13, 1096, 1566, 2271, 2438, 3192
BstNSI	RCATGY	5 cut(s)	530, 677, 939, 3172, 3638
BstPAI	GACNNNNGTC	1 cut(s)	3606
BstPI	GGTNACC	2 cut(s)	2159, 2779
BstSCI	CCNGG	8 cut(s)	11, 752, 1094, 1564, 2269, 2436, 2983, 3190
BstSFI	CTRYAG	5 cut(s)	315, 552, 1838, 3498, 3592
BstUI	CGCG	3 cut(s)	36, 245, 1453
BstV2I	GAAGAC	6 cut(s)	365, 608, 676, 2105, 3184, 3743
BstX2I	RGATCY	2 cut(s)	254, 1718
BstXI	CCANNNNNNTGG	1 cut(s)	247
BstYI	RGATCY	2 cut(s)	254, 1718
BsuI	GTATCC	2 cut(s)	610, 1078
BsuRI	GGCC	9 cut(s)	47, 121, 284, 1129, 1621, 2864, 2920, 3301, 3320
BtgZI	GCGATG	1 cut(s)	657
BtrI	CACGTC	1 cut(s)	3182
BtsI	GCAGTG	3 cut(s)	413, 549, 1739
BtsIMutI	CAGTG	6 cut(s)	204, 413, 450, 549, 1722, 1739
BveI	ACCTGC	4 cut(s)	886, 1466, 2147, 3637
Cac8I	GCNNGC	6 cut(s)	126, 339, 1451, 2067, 2623, 3006
CaiI	CAGNNNCTG	4 cut(s)	694, 878, 1481, 1741
CfoI	GCGC	6 cut(s)	18, 38, 247, 1455, 2593, 3640
Cfr10I	RCCGGY	1 cut(s)	124
Cfr13I	GGNCC	9 cut(s)	119, 140, 159, 799, 1469, 1832, 1868, 2862, 2978
CseI	GACGC	4 cut(s)	69, 822, 2669, 3130
DraI	TTTAAA	3 cut(s)	1279, 1789, 3631
Eam1104I	CTCTTC	6 cut(s)	31, 930, 1560, 1863, 2180, 2916
EarI	CTCTTC	6 cut(s)	31, 930, 1560, 1863, 2180, 2916
EciI	GGCGGA	4 cut(s)	143, 186, 3578, 3738
Ecl136II	GAGCTC	2 cut(s)	181, 3691
Eco130I	CCWWGG	9 cut(s)	285, 1500, 1587, 1616, 2031, 2341, 2865, 3338, 3405
Eco147I	AGGCCT	4 cut(s)	284, 1621, 2920, 3301
Eco24I	GRGCYC	3 cut(s)	183, 1451, 3693
Eco31I	GGTCTC	2 cut(s)	1108, 1490
Eco32I	GATATC	1 cut(s)	1257
Eco47I	GGWCC	7 cut(s)	140, 159, 799, 1469, 1832, 1868, 2978
Eco47III	AGCGCT	1 cut(s)	2592
Eco53kI	GAGCTC	2 cut(s)	181, 3691
Eco57I	CTGAAG	2 cut(s)	2897, 3045
Eco88I	CYCGRG	1 cut(s)	3581
Eco91I	GGTNACC	2 cut(s)	2159, 2779
EcoICRI	GAGCTC	2 cut(s)	181, 3691
EcoNI	CCTNNNNNAGG	2 cut(s)	2958, 3314
EcoO109I	RGGNCCY	3 cut(s)	159, 799, 2862
EcoO65I	GGTNACC	2 cut(s)	2159, 2779
EcoRI	GAATTC	2 cut(s)	373, 1299
EcoRII	CCWGG	6 cut(s)	11, 1094, 1564, 2269, 2436, 3190
EcoRV	GATATC	1 cut(s)	1257
EcoT14I	CCWWGG	9 cut(s)	285, 1500, 1587, 1616, 2031, 2341, 2865, 3338, 3405
EcoT22I	ATGCAT	4 cut(s)	679, 780, 2173, 2652
EcoT38I	GRGCYC	3 cut(s)	183, 1451, 3693
ErhI	CCWWGG	9 cut(s)	285, 1500, 1587, 1616, 2031, 2341, 2865, 3338, 3405
Esp3I	CGTCTC	1 cut(s)	3747
FalI	AAGNNNNNCTT	8 cut(s)	1884, 1916, 2101, 2133, 3052, 3084, 3541, 3573
FaqI	GGGAC	4 cut(s)	145, 812, 1881, 2248
FauI	CCCGC	4 cut(s)	319, 330, 429, 1458
FauNDI	CATATG	2 cut(s)	780, 1876
FbaI	TGATCA	1 cut(s)	997
FblI	GTMKAC	1 cut(s)	3674
FriOI	GRGCYC	3 cut(s)	183, 1451, 3693
FspAI	RTGCGCAY	1 cut(s)	3639
FspI	TGCGCA	1 cut(s)	3639
GlaI	GCGC	6 cut(s)	17, 37, 246, 1454, 2592, 3639
GsaI	CCCAGC	2 cut(s)	1668, 2629
GsuI	CTGGAG	3 cut(s)	269, 747, 2064
HaeII	RGCGCY	1 cut(s)	2594
HaeIII	GGCC	9 cut(s)	47, 121, 284, 1129, 1621, 2864, 2920, 3301, 3320
HapII	CCGG	7 cut(s)	125, 170, 272, 754, 2333, 2784, 2984
HgaI	GACGC	4 cut(s)	69, 822, 2669, 3130
HhaI	GCGC	6 cut(s)	18, 38, 247, 1455, 2593, 3640
Hin1I	GRCGYC	2 cut(s)	80, 3740
Hin6I	GCGC	6 cut(s)	16, 36, 245, 1453, 2591, 3638
HinP1I	GCGC	6 cut(s)	16, 36, 245, 1453, 2591, 3638
HincII	GTYRAC	2 cut(s)	3163, 3675
HindII	GTYRAC	2 cut(s)	3163, 3675
HindIII	AAGCTT	3 cut(s)	347, 2192, 2445
HinfI	GANTC	8 cut(s)	152, 209, 1550, 1592, 1598, 2964, 3484, 3671
HpaII	CCGG	7 cut(s)	125, 170, 272, 754, 2333, 2784, 2984
Hpy166II	GTNNAC	8 cut(s)	518, 549, 2078, 2286, 3163, 3238, 3355, 3675
Hpy8I	GTNNAC	8 cut(s)	518, 549, 2078, 2286, 3163, 3238, 3355, 3675
Hpy99I	CGWCG	2 cut(s)	97, 100
HpyCH4III	ACNGT	5 cut(s)	445, 454, 581, 1726, 3593
HpyCH4IV	ACGT	5 cut(s)	3099, 3181, 3271, 3426, 3740
HpySE526I	ACGT	5 cut(s)	3099, 3181, 3271, 3426, 3740
Hsp92I	GRCGYC	2 cut(s)	80, 3740
HspAI	GCGC	6 cut(s)	16, 36, 245, 1453, 2591, 3638
KflI	GGGWCCC	2 cut(s)	159, 799
Kpn2I	TCCGGA	2 cut(s)	169, 2332
KroI	GCCGGC	1 cut(s)	124
KroNI	GCCGGC	1 cut(s)	126
Ksp22I	TGATCA	1 cut(s)	997
LmnI	GCTCC	9 cut(s)	26, 130, 186, 766, 1446, 2364, 2476, 3196, 3696
MaeII	ACGT	5 cut(s)	3099, 3181, 3271, 3426, 3740
MfeI	CAATTG	1 cut(s)	2942
MflI	RGATCY	2 cut(s)	254, 1718
MhlI	GDGCHC	4 cut(s)	183, 413, 1451, 3693
MlyI	GAGTC	1 cut(s)	3680
MmeI	TCCRAC	3 cut(s)	208, 2223, 2454
Mph1103I	ATGCAT	4 cut(s)	679, 780, 2173, 2652
MreI	CGCCGGCG	1 cut(s)	124
MroI	TCCGGA	2 cut(s)	169, 2332
MroNI	GCCGGC	1 cut(s)	124
MslI	CAYNNNNRTG	4 cut(s)	971, 1527, 3053, 3639
MspA1I	CMGCKG	2 cut(s)	694, 2431
MspCI	CTTAAG	2 cut(s)	2705, 2768
MspI	CCGG	7 cut(s)	125, 170, 272, 754, 2333, 2784, 2984
MspR9I	CCNGG	8 cut(s)	13, 754, 1096, 1566, 2271, 2438, 2985, 3192
MunI	CAATTG	1 cut(s)	2942
Mva1269I	GAATGC	4 cut(s)	399, 726, 763, 2733
MvaI	CCWGG	6 cut(s)	13, 1096, 1566, 2271, 2438, 3192
MvnI	CGCG	3 cut(s)	36, 245, 1453
NaeI	GCCGGC	1 cut(s)	126
NciI	CCSGG	2 cut(s)	754, 2985
NdeI	CATATG	2 cut(s)	780, 1876
NgoMIV	GCCGGC	1 cut(s)	124
NmuCI	GTSAC	6 cut(s)	861, 890, 1796, 2419, 2636, 3473
NsbI	TGCGCA	1 cut(s)	3639
NsiI	ATGCAT	4 cut(s)	679, 780, 2173, 2652
NspI	RCATGY	5 cut(s)	530, 677, 939, 3172, 3638
NspV	TTCGAA	1 cut(s)	377
PceI	AGGCCT	4 cut(s)	284, 1621, 2920, 3301
PciI	ACATGT	1 cut(s)	526
PctI	GAATGC	4 cut(s)	399, 726, 763, 2733
PdiI	GCCGGC	1 cut(s)	126
PfeI	GAWTC	7 cut(s)	152, 209, 1550, 1592, 1598, 2964, 3484
PflFI	GACNNNGTC	1 cut(s)	1802
PflMI	CCANNNNNTGG	2 cut(s)	770, 1877
PleI	GAGTC	1 cut(s)	3679
PpsI	GAGTC	1 cut(s)	3679
PpuMI	RGGWCCY	2 cut(s)	159, 799
PscI	ACATGT	1 cut(s)	526
PshAI	GACNNNNGTC	1 cut(s)	3606
PshBI	ATTAAT	2 cut(s)	993, 2580
Psp124BI	GAGCTC	2 cut(s)	183, 3693
Psp5II	RGGWCCY	2 cut(s)	159, 799
Psp6I	CCWGG	6 cut(s)	11, 1094, 1564, 2269, 2436, 3190
PspEI	GGTNACC	2 cut(s)	2159, 2779
PspFI	CCCAGC	2 cut(s)	1664, 2625
PspGI	CCWGG	6 cut(s)	11, 1094, 1564, 2269, 2436, 3190
PspPI	GGNCC	9 cut(s)	119, 140, 159, 799, 1469, 1832, 1868, 2862, 2978
PspPPI	RGGWCCY	2 cut(s)	159, 799
PstI	CTGCAG	4 cut(s)	319, 556, 1842, 3502
PstNI	CAGNNNCTG	4 cut(s)	694, 878, 1481, 1741
PsuI	RGATCY	2 cut(s)	254, 1718
PsyI	GACNNNGTC	1 cut(s)	1802
PvuII	CAGCTG	2 cut(s)	694, 2431
RseI	CAYNNNNRTG	4 cut(s)	971, 1527, 3053, 3639
SacI	GAGCTC	2 cut(s)	183, 3693
SalI	GTCGAC	1 cut(s)	3673
Sau96I	GGNCC	9 cut(s)	119, 140, 159, 799, 1469, 1832, 1868, 2862, 2978
ScaI	AGTACT	3 cut(s)	505, 985, 1424
SchI	GAGTC	1 cut(s)	3680
ScrFI	CCNGG	8 cut(s)	13, 754, 1096, 1566, 2271, 2438, 2985, 3192
SduI	GDGCHC	4 cut(s)	183, 413, 1451, 3693
SfcI	CTRYAG	5 cut(s)	315, 552, 1838, 3498, 3592
SfuI	TTCGAA	1 cut(s)	377
SgrAI	CRCCGGYG	1 cut(s)	124
SinI	GGWCC	7 cut(s)	140, 159, 799, 1469, 1832, 1868, 2978
SmiMI	CAYNNNNRTG	4 cut(s)	971, 1527, 3053, 3639
SmlI	CTYRAG	4 cut(s)	176, 2084, 2705, 2768
SmoI	CTYRAG	4 cut(s)	176, 2084, 2705, 2768
SseBI	AGGCCT	4 cut(s)	284, 1621, 2920, 3301
SspI	AATATT	1 cut(s)	828
SstI	GAGCTC	2 cut(s)	183, 3693
StuI	AGGCCT	4 cut(s)	284, 1621, 2920, 3301
StyD4I	CCNGG	8 cut(s)	11, 752, 1094, 1564, 2269, 2436, 2983, 3190
StyI	CCWWGG	9 cut(s)	285, 1500, 1587, 1616, 2031, 2341, 2865, 3338, 3405
TaaI	ACNGT	5 cut(s)	445, 454, 581, 1726, 3593
TaiI	ACGT	5 cut(s)	3102, 3184, 3274, 3429, 3743
TaqI	TCGA	5 cut(s)	98, 377, 940, 1885, 3674
TatI	WGTACW	4 cut(s)	503, 983, 1422, 3588
TauI	GCSGC	3 cut(s)	124, 298, 3334
TfiI	GAWTC	7 cut(s)	152, 209, 1550, 1592, 1598, 2964, 3484
TscAI	CASTG	6 cut(s)	211, 413, 457, 556, 1729, 1746
TseFI	GTSAC	6 cut(s)	861, 890, 1796, 2419, 2636, 3473
Tsp45I	GTSAC	6 cut(s)	861, 890, 1796, 2419, 2636, 3473
TspGWI	ACGGA	4 cut(s)	120, 311, 1127, 3470
TspRI	CASTG	6 cut(s)	211, 413, 457, 556, 1729, 1746
Tth111I	GACNNNGTC	1 cut(s)	1802
Van91I	CCANNNNNTGG	2 cut(s)	770, 1877
Vha464I	CTTAAG	2 cut(s)	2705, 2768
VpaK11BI	GGWCC	7 cut(s)	140, 159, 799, 1469, 1832, 1868, 2978
VspI	ATTAAT	2 cut(s)	993, 2580
XagI	CCTNNNNNAGG	2 cut(s)	2958, 3314
XapI	RAATTY	7 cut(s)	373, 1299, 1396, 1808, 2291, 2792, 3522
XbaI	TCTAGA	1 cut(s)	961
XceI	RCATGY	5 cut(s)	530, 677, 939, 3172, 3638
XcmI	CCANNNNNNNNNTGG	1 cut(s)	3412
XmiI	GTMKAC	1 cut(s)	3674
ZraI	GACGTC	1 cut(s)	3741
ZrmI	AGTACT	3 cut(s)	505, 985, 1424
Zsp2I	ATGCAT	4 cut(s)	679, 780, 2173, 2652

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.