Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0006487

Physical Location & Seq

Genomic Coordinates Forward (+)

42016462 .. 42023918

Gene Structure 7457 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1305300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2199 bp

ATGTTTAGCGCTTCTTCTAGTGCCAATTTTGACTTGACTGGCAGCACTTCTAAAAAACTTGACGAGCAAAGGGAAATCAGAGAACTTCTTCACAACTTTGATCCTCCAATGTCTATATCAACTAGACGACAACGTCGACAAGCATTTAAAGAAAAACTGCAACAAGGAATTCTACGTCCTGGTGATACTGATGTCTCATTCGAAAGTTTTCCATATTACCTAAGTGATACAACAAAGAAGGTTTTGATTGCATCCACCCTCCCGAATTTGAGGTGTAGCAAGTTTGGAAAATATTTTTCATCACTACCTACCGGGAGCCCGCGCATATTGTTATCTGGTCCAGCAGGTTCTGAGATATATCAGGAAACCTTGGCAAAGGCACTCGCGAAACATTTTGATGCTAGATTACTAATTGTAGATTCTCTTCTCCGGCATGGTGTATGTTCCTTCGTTGATGAAAGTGTTTCCACTCATGCAATGCGTGAACTTCTTGAGGTACTCATCTCCTTGTGTGGTTCTGGAGGTGATGATATTGGCAATCTTGCAATCGGTGAACTTCTTGAGGTGGCAACCAATATCTGCACCAATGTTGTGCAGGCGGCAACCAATGAGAGTAAAAGTCTTCCTTTGATAGTGTTTTTGAAAGATATAGAATCGGTTATGGTAGGTAACCCAGATGCATATAGTGTCTTGAAGAGTAAGCTTGAAAACTTGCCAGAGAATGTTGTTGTGATCGGCTCTCATACCCAGTTGGACAATCGCAAGGACAAGGATAACCTTGGGGGTAGGCTGCATGACAGGAGCAAAGAAACTCCTAAAACTATGAAGCAAGTTCAACTCTCTCGGATTTTCCCCAACAAAGTGACCATGCCCCAGGATGAGGCTTTACTTTCGGACTGGAAGCAGCAGTTTGAACGTGATGTTGAAACTTTGAAATCGAAGTTTTATATTGTTGCCATTCACTCAGTTCTTAACCGAGTTTCTGTGGATTGCCCTGACCTTGAAAGTCTGTGCATTAAAGATCTAGTGCTTACAACTGAAAGTGTTGACAAAGTAGTAGGCTGGGCTGTTAGTCACCATCTTATGCATTGTTCAGACGCTTTAGTTAAAGATGACAAACTTGTTATTTCTACTGAAAGTCTTAGGGATGGACTAAACATTCTACAAGGCATTCAAAACGAAAACAAGAGCATAAAGAAATCACTTAAGGATGTGGTTACTGGAAATGAATTTGAGAAAAAACTTCTTGCTGATGTTATTCCACCGAGCGATATTGGGGTTAGTTTTGATGACATCGGGGCCTTGGAAAATGTGAAGGACACCCTAAAGGAGTTGGTGATGCTTCCACTTCAGAGGCCTGAATTATTTACCAAGGGACAGTTGAATAAGCCTTGTAAAGGAATCTTGCTATTTGGTCCCCCGGGTACTGGAAAGACTATGCTTGCGAAGGCTGTTGCAACTGAAGCTGGTGCAAACTTTATTAACATATCAATGTCAAGTATTACTTCAGAGTGGTTTGGGGAAGGAGAGAAGTACGTTAAAGCGGTGTTCTCCTTAGCTAGTAAAATTGCCCCTAGTGTTGTTTTTGTTGATGAGGTGGACAGCATGTTAGGCAGACGTGAAAATCCTGGGGAACATGAGGCTATGCGTAAAATGAAAAATGAGTTCATGGTTAACTGGGATGGTCTGCGTACAAAGGATAAAGAACGTGTATTGGTACTTGCTGCTACTAATAGGCCTTTTGACCTTGATGAGGCTGTTATTAGGAGGCTTCCGAGGAGATTGATGGTGAACTTGCCAGATGCCCAGAACAGAGAAAAAATACTGAGAGTTGTATTGGCCAAAGAAGATTTGGAAACTGACGTTGATTTGGAGGCAGTTGCAAATATGACAGATGGGTATTCCGGAAGTGACTTAAAGAATCTGTGTGTTGCTGCAGCTCACCTCCCCATTAGGGAAATTTTAGAGAAGGAGAAAAAGGAGAGAAGTTTAGCTTTGGCGGAGAACAGACCTGTACCGGAGTTGTACTGCAGCACCGACATTCGTCCTTTGAAGATGGAGGACTTCAAACATGCGCATGAGCAGGTGTATGCAAGTGTGTCTTCGGAGTCGACAAACATGAGTGAGCTCCTCCAATGGAATGACCTATACGGCGAAGGCAGATCAAGAAAGAAGACGTCTCTCAGCTATTTTATATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002090 GO:0002092 GO:0003008 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0007610 GO:0007611 GO:0007612 GO:0007613 GO:0008150 GO:0009893 GO:0010604 GO:0010646 GO:0010648 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019222 GO:0023051 GO:0023057 GO:0030100 GO:0031323 GO:0031325 GO:0032501 GO:0032879 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044456 GO:0044459 GO:0044464 GO:0045202 GO:0045211 GO:0045807 GO:0048259 GO:0048260 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050804 GO:0050805 GO:0050877 GO:0050890 GO:0051049 GO:0051050 GO:0051128 GO:0051130 GO:0051966 GO:0051967 GO:0060255 GO:0060627 GO:0065007 GO:0071944 GO:0097060 GO:0098590 GO:0098794 GO:0099177

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA FHA

Protein Analysis

733

Amino Acids

81.63

Weight (kDa)

5.98

Isoelectric Point (pI)

36.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF7751	PF24933	329 - 389	1.7e-26	Domain of unknown function (DUF7751)
AAA	PF00004	468 - 598	8.7e-38	ATPase family associated with various cellular activities (AAA)
AAA_lid_3	PF17862	621 - 657	1e-13	AAA+ lid domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006487)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G02890 AT1G02890 AT4G02480
fragaria_vesca	FvH4_2g25490 FvH4_2g25490 FvH4_2g25490
malus_domestica	MD03G1285800.v1.1 MD11G1305200.v1.1 MD11G1305300.v1.1 MD11G1305400.v1.1
prunus_persica	Prupe.8G259000_v2.0.a1 Prupe.8G259000_v2.0.a1 Prupe.8G259000_v2.0.a1 Prupe.8G259000_v2.0.a1
pyrus_communis	pycom03g22650 pycom11g26700 pycom11g26720
rosa_chinensis	RchiOBHm_Chr6g0293701
rosa_laevigata	RLG00000011931
rosa_roxburghii	Rroxscaffold_7G00173960
rosa_rugosa	Rorug06G0237500
rosa_samantha	Rh6AG350200 Rh6BG357700 Rh6CG364500 Rh6DG351400
rosa_wichuraiana	Rw6G030490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2074
AasI	GACNNNNNNGTC	1 cut(s)	132
AatII	GACGTC	1 cut(s)	2180
Acc16I	TGCGCA	1 cut(s)	2076
Acc36I	ACCTGC	2 cut(s)	335, 2074
AccI	GTMKAC	2 cut(s)	136, 2111
AccII	CGCG	2 cut(s)	322, 386
AccIII	TCCGGA	1 cut(s)	1904
AciI	CCGC	4 cut(s)	320, 599, 1544, 2000
AclWI	GGATC	1 cut(s)	95
AcoI	YGGCCR	1 cut(s)	1839
AcsI	RAATTY	4 cut(s)	168, 265, 1229, 1959
AcuI	CTGAAG	3 cut(s)	1334, 1482, 1491
AcyI	GRCGYC	1 cut(s)	2177
AfaI	GTAC	7 cut(s)	498, 1426, 1535, 1693, 1719, 2016, 2027
AfeI	AGCGCT	1 cut(s)	10
AfiI	CCNNNNNNNGG	5 cut(s)	880, 1397, 1427, 1753, 1954
AflII	CTTAAG	1 cut(s)	1205
AflIII	ACRYGT	1 cut(s)	1708
AjiI	CACGTC	1 cut(s)	1619
AjnI	CCWGG	3 cut(s)	178, 873, 1627
AluBI	AGCT	7 cut(s)	703, 1466, 1559, 1940, 1994, 2128, 2187
AluI	AGCT	7 cut(s)	703, 1466, 1559, 1940, 1994, 2128, 2187
Alw21I	GWGCWC	1 cut(s)	2130
Alw26I	GTCTC	2 cut(s)	199, 2184
AlwI	GGATC	1 cut(s)	95
AlwNI	CAGNNNCTG	1 cut(s)	350
Ama87I	CYCGRG	1 cut(s)	1420
Aor13HI	TCCGGA	1 cut(s)	1904
Aor51HI	AGCGCT	1 cut(s)	10
AoxI	GGCC	4 cut(s)	1299, 1355, 1736, 1839
ApeKI	GCWGC	7 cut(s)	42, 790, 904, 1724, 1934, 1937, 2031
ApoI	RAATTY	4 cut(s)	168, 265, 1229, 1959
Asp700I	GAANNNNTTC	2 cut(s)	87, 207
AspLEI	GCGC	3 cut(s)	11, 324, 2077
AspS9I	GGNCC	3 cut(s)	338, 1299, 1415
AsuC2I	CCSGG	3 cut(s)	313, 1421, 1422
AsuHPI	GGTGA	7 cut(s)	194, 536, 563, 1067, 1348, 1801, 1934
AsuII	TTCGAA	1 cut(s)	201
AvaI	CYCGRG	1 cut(s)	1420
AvaII	GGWCC	2 cut(s)	338, 1415
BaeI	ACNNNNGTAYC	2 cut(s)	177, 210
BalI	TGGCCA	1 cut(s)	1841
BanII	GRGCYC	2 cut(s)	320, 2130
BarI	GAAGNNNNNNTAC	2 cut(s)	408, 440
BbsI	GAAGAC	3 cut(s)	614, 2094, 2180
Bbv12I	GWGCWC	1 cut(s)	2130
BbvI	GCAGC	7 cut(s)	54, 777, 916, 1711, 1921, 1949, 2043
BccI	CCATC	6 cut(s)	1086, 1142, 1676, 1780, 1889, 2050
BceAI	ACGGC	1 cut(s)	2167
BciT130I	CCWGG	3 cut(s)	180, 875, 1629
BcnI	CCSGG	3 cut(s)	313, 1421, 1422
BcoDI	GTCTC	2 cut(s)	199, 2184
BfaI	CTAG	6 cut(s)	18, 123, 402, 1025, 1560, 1575
BfmI	CTRYAG	2 cut(s)	1935, 2029
BfoI	RGCGCY	1 cut(s)	12
BfrI	CTTAAG	1 cut(s)	1205
BfuAI	ACCTGC	2 cut(s)	335, 2074
BglII	AGATCT	1 cut(s)	1021
BisI	GCNGC	8 cut(s)	43, 600, 791, 905, 1725, 1935, 1938, 2032
BlsI	GCNGC	8 cut(s)	44, 601, 792, 906, 1726, 1936, 1939, 2033
Bme1390I	CCNGG	6 cut(s)	180, 313, 875, 1421, 1422, 1629
Bme18I	GGWCC	2 cut(s)	338, 1415
BmeT110I	CYCGRG	1 cut(s)	1420
BmgBI	CACGTC	1 cut(s)	1619
BmgT120I	GGNCC	3 cut(s)	338, 1299, 1415
BmiI	GGNNCC	3 cut(s)	317, 1300, 1417
BmrFI	CCNGG	6 cut(s)	180, 313, 875, 1421, 1422, 1629
BmrI	ACTGGG	2 cut(s)	742, 1687
BmsI	GCATC	5 cut(s)	260, 388, 667, 1329, 1792
BmuI	ACTGGG	2 cut(s)	742, 1687
BoxI	GACNNNNGTC	1 cut(s)	2043
BpiI	GAAGAC	3 cut(s)	614, 2094, 2180
BpmI	CTGGAG	1 cut(s)	540
Bpu10I	CCTNAGC	1 cut(s)	1555
Bpu14I	TTCGAA	1 cut(s)	201
BpuEI	CTTGAG	2 cut(s)	512, 581
BpuMI	CCSGG	3 cut(s)	313, 1421, 1422
BsaHI	GRCGYC	1 cut(s)	2177
BsaJI	CCNNGG	9 cut(s)	369, 778, 873, 1302, 1371, 1419, 1420, 1628, 1775
BsaWI	WCCGGW	2 cut(s)	1904, 2017
Bsc4I	CCNNNNNNNGG	5 cut(s)	880, 1397, 1427, 1753, 1954
Bse1I	ACTGG	6 cut(s)	43, 748, 902, 1225, 1432, 1682
Bse3DI	GCAATG	1 cut(s)	483
BseAI	TCCGGA	1 cut(s)	1904
BseBI	CCWGG	3 cut(s)	180, 875, 1629
BseDI	CCNNGG	9 cut(s)	369, 778, 873, 1302, 1371, 1419, 1420, 1628, 1775
BseGI	GGATG	5 cut(s)	251, 883, 1153, 1216, 1687
BseLI	CCNNNNNNNGG	5 cut(s)	880, 1397, 1427, 1753, 1954
BseMI	GCAATG	1 cut(s)	483
BseMII	CTCAG	4 cut(s)	342, 978, 1817, 2197
BseNI	ACTGG	6 cut(s)	43, 748, 902, 1225, 1432, 1682
BseRI	GAGGAG	2 cut(s)	1792, 2120
BseXI	GCAGC	7 cut(s)	54, 777, 916, 1711, 1921, 1949, 2043
BseYI	CCCAGC	1 cut(s)	1062
BsgI	GTGCAG	2 cut(s)	565, 614
Bsh1236I	CGCG	2 cut(s)	322, 386
BshFI	GGCC	4 cut(s)	1301, 1357, 1738, 1841
BsiHKAI	GWGCWC	1 cut(s)	2130
BsiHKCI	CYCGRG	1 cut(s)	1420
BsiSI	CCGG	5 cut(s)	312, 430, 1421, 1905, 2018
BslFI	GGGAC	2 cut(s)	1389, 1401
BslI	CCNNNNNNNGG	5 cut(s)	880, 1397, 1427, 1753, 1954
BsmAI	GTCTC	2 cut(s)	199, 2184
BsmBI	CGTCTC	1 cut(s)	2184
BsmFI	GGGAC	2 cut(s)	1389, 1401
BsmI	GAATGC	1 cut(s)	1170
BsnI	GGCC	4 cut(s)	1301, 1357, 1738, 1841
BsoBI	CYCGRG	1 cut(s)	1420
Bsp119I	TTCGAA	1 cut(s)	201
Bsp1286I	GDGCHC	2 cut(s)	320, 2130
Bsp13I	TCCGGA	1 cut(s)	1904
Bsp143I	GATC	4 cut(s)	100, 732, 1021, 2162
Bsp68I	TCGCGA	1 cut(s)	386
BspACI	CCGC	4 cut(s)	320, 599, 1544, 2000
BspANI	GGCC	4 cut(s)	1301, 1357, 1738, 1841
BspCNI	CTCAG	4 cut(s)	343, 977, 1818, 2196
BspEI	TCCGGA	1 cut(s)	1904
BspFNI	CGCG	2 cut(s)	322, 386
BspLI	GGNNCC	3 cut(s)	317, 1300, 1417
BspMAI	CTGCAG	2 cut(s)	1939, 2033
BspMI	ACCTGC	2 cut(s)	335, 2074
BspPI	GGATC	1 cut(s)	95
BspT104I	TTCGAA	1 cut(s)	201
BspTI	CTTAAG	1 cut(s)	1205
BsrDI	GCAATG	1 cut(s)	483
BsrI	ACTGG	6 cut(s)	43, 748, 902, 1225, 1432, 1682
BssECI	CCNNGG	9 cut(s)	369, 778, 873, 1302, 1371, 1419, 1420, 1628, 1775
BssMI	GATC	4 cut(s)	100, 732, 1021, 2162
BssNI	GRCGYC	1 cut(s)	2177
BssT1I	CCWWGG	4 cut(s)	369, 778, 1302, 1371
Bst2UI	CCWGG	3 cut(s)	180, 875, 1629
Bst4CI	ACNGT	1 cut(s)	1380
Bst6I	CTCTTC	2 cut(s)	429, 689
BstACI	GRCGYC	1 cut(s)	2177
BstAFI	CTTAAG	1 cut(s)	1205
BstBI	TTCGAA	1 cut(s)	201
BstC8I	GCNNGC	3 cut(s)	320, 597, 1443
BstDEI	CTNAG	7 cut(s)	221, 351, 964, 1142, 1555, 1826, 2183
BstEII	GGTNACC	1 cut(s)	668
BstENI	CCTNNNNNAGG	2 cut(s)	1395, 1751
BstF5I	GGATG	5 cut(s)	251, 883, 1153, 1216, 1687
BstFNI	CGCG	2 cut(s)	322, 386
BstH2I	RGCGCY	1 cut(s)	12
BstHHI	GCGC	3 cut(s)	11, 324, 2077
BstKTI	GATC	4 cut(s)	103, 735, 1024, 2165
BstMAI	GTCTC	2 cut(s)	199, 2184
BstMBI	GATC	4 cut(s)	100, 732, 1021, 2162
BstMWI	GCNNNNNNNGC	2 cut(s)	1463, 1611
BstNI	CCWGG	3 cut(s)	180, 875, 1629
BstNSI	RCATGY	2 cut(s)	1609, 2075
BstPAI	GACNNNNGTC	1 cut(s)	2043
BstPI	GGTNACC	1 cut(s)	668
BstSCI	CCNGG	6 cut(s)	178, 311, 873, 1419, 1420, 1627
BstSFI	CTRYAG	2 cut(s)	1935, 2029
BstUI	CGCG	2 cut(s)	322, 386
BstV1I	GCAGC	7 cut(s)	54, 777, 916, 1711, 1921, 1949, 2043
BstV2I	GAAGAC	3 cut(s)	614, 2094, 2180
BstX2I	RGATCY	1 cut(s)	1021
BstYI	RGATCY	1 cut(s)	1021
BsuRI	GGCC	4 cut(s)	1301, 1357, 1738, 1841
BtrI	CACGTC	1 cut(s)	1619
BtsCI	GGATG	5 cut(s)	251, 883, 1153, 1216, 1687
BtuMI	TCGCGA	1 cut(s)	386
BveI	ACCTGC	2 cut(s)	335, 2074
Cac8I	GCNNGC	3 cut(s)	320, 597, 1443
CaiI	CAGNNNCTG	1 cut(s)	350
CfoI	GCGC	3 cut(s)	11, 324, 2077
Cfr13I	GGNCC	3 cut(s)	338, 1299, 1415
Cfr9I	CCCGGG	1 cut(s)	1420
CseI	GACGC	1 cut(s)	1106
Csp6I	GTAC	7 cut(s)	497, 1425, 1534, 1692, 1718, 2015, 2026
CviQI	GTAC	7 cut(s)	497, 1425, 1534, 1692, 1718, 2015, 2026
DdeI	CTNAG	7 cut(s)	221, 351, 964, 1142, 1555, 1826, 2183
DpnI	GATC	4 cut(s)	102, 734, 1023, 2164
DpnII	GATC	4 cut(s)	100, 732, 1021, 2162
DraI	TTTAAA	1 cut(s)	148
DrdI	GACNNNNNNGTC	1 cut(s)	132
DseDI	GACNNNNNNGTC	1 cut(s)	132
EaeI	YGGCCR	1 cut(s)	1839
Eam1104I	CTCTTC	2 cut(s)	429, 689
EarI	CTCTTC	2 cut(s)	429, 689
EciI	GGCGGA	1 cut(s)	2015
Ecl136II	GAGCTC	1 cut(s)	2128
Eco130I	CCWWGG	4 cut(s)	369, 778, 1302, 1371
Eco147I	AGGCCT	2 cut(s)	1357, 1738
Eco24I	GRGCYC	2 cut(s)	320, 2130
Eco47I	GGWCC	2 cut(s)	338, 1415
Eco47III	AGCGCT	1 cut(s)	10
Eco53kI	GAGCTC	1 cut(s)	2128
Eco57I	CTGAAG	3 cut(s)	1334, 1482, 1491
Eco88I	CYCGRG	1 cut(s)	1420
Eco91I	GGTNACC	1 cut(s)	668
EcoICRI	GAGCTC	1 cut(s)	2128
EcoNI	CCTNNNNNAGG	2 cut(s)	1395, 1751
EcoO109I	RGGNCCY	1 cut(s)	1299
EcoO65I	GGTNACC	1 cut(s)	668
EcoRI	GAATTC	1 cut(s)	168
EcoRII	CCWGG	3 cut(s)	178, 873, 1627
EcoT14I	CCWWGG	4 cut(s)	369, 778, 1302, 1371
EcoT22I	ATGCAT	2 cut(s)	682, 1089
EcoT38I	GRGCYC	2 cut(s)	320, 2130
ErhI	CCWWGG	4 cut(s)	369, 778, 1302, 1371
Esp3I	CGTCTC	1 cut(s)	2184
FalI	AAGNNNNNCTT	8 cut(s)	610, 642, 1489, 1521, 1978, 2010, 2086, 2118
FaqI	GGGAC	2 cut(s)	1389, 1401
FauI	CCCGC	1 cut(s)	327
FblI	GTMKAC	2 cut(s)	136, 2111
Fnu4HI	GCNGC	8 cut(s)	43, 600, 791, 905, 1725, 1935, 1938, 2032
FokI	GGATG	5 cut(s)	238, 890, 1160, 1223, 1694
FriOI	GRGCYC	2 cut(s)	320, 2130
Fsp4HI	GCNGC	8 cut(s)	43, 600, 791, 905, 1725, 1935, 1938, 2032
FspAI	RTGCGCAY	1 cut(s)	2076
FspBI	CTAG	6 cut(s)	18, 123, 402, 1025, 1560, 1575
FspI	TGCGCA	1 cut(s)	2076
GlaI	GCGC	3 cut(s)	10, 323, 2076
GluI	GCNGC	8 cut(s)	43, 600, 791, 905, 1725, 1935, 1938, 2032
GsaI	CCCAGC	1 cut(s)	1066
GsuI	CTGGAG	1 cut(s)	540
HaeII	RGCGCY	1 cut(s)	12
HaeIII	GGCC	4 cut(s)	1301, 1357, 1738, 1841
HapII	CCGG	5 cut(s)	312, 430, 1421, 1905, 2018
HgaI	GACGC	1 cut(s)	1106
HhaI	GCGC	3 cut(s)	11, 324, 2077
Hin1I	GRCGYC	1 cut(s)	2177
Hin6I	GCGC	3 cut(s)	9, 322, 2075
HinP1I	GCGC	3 cut(s)	9, 322, 2075
HincII	GTYRAC	4 cut(s)	137, 1048, 1675, 2112
HindII	GTYRAC	4 cut(s)	137, 1048, 1675, 2112
HindIII	AAGCTT	1 cut(s)	701
HinfI	GANTC	5 cut(s)	419, 653, 1401, 1921, 2108
HpaI	GTTAAC	1 cut(s)	1675
HpaII	CCGG	5 cut(s)	312, 430, 1421, 1905, 2018
HphI	GGTGA	7 cut(s)	194, 536, 563, 1067, 1348, 1801, 1934
Hpy166II	GTNNAC	8 cut(s)	137, 485, 554, 1048, 1600, 1675, 1792, 2112
Hpy188I	TCNGA	9 cut(s)	80, 352, 846, 895, 1096, 1353, 1510, 1776, 2107
Hpy188III	TCNNGA	9 cut(s)	262, 362, 385, 491, 519, 560, 691, 1905, 2166
Hpy8I	GTNNAC	8 cut(s)	137, 485, 554, 1048, 1600, 1675, 1792, 2112
Hpy99I	CGWCG	1 cut(s)	138
HpyAV	CCTTC	7 cut(s)	232, 457, 1309, 1441, 1517, 1963, 2150
HpyCH4III	ACNGT	1 cut(s)	1380
HpyCH4IV	ACGT	8 cut(s)	133, 175, 916, 1536, 1618, 1708, 1863, 2177
HpyF10VI	GCNNNNNNNGC	2 cut(s)	1463, 1611
HpyF3I	CTNAG	7 cut(s)	221, 351, 964, 1142, 1555, 1826, 2183
HpySE526I	ACGT	8 cut(s)	133, 175, 916, 1536, 1618, 1708, 1863, 2177
Hsp92I	GRCGYC	1 cut(s)	2177
HspAI	GCGC	3 cut(s)	9, 322, 2075
Kpn2I	TCCGGA	1 cut(s)	1904
KspAI	GTTAAC	1 cut(s)	1675
Kzo9I	GATC	4 cut(s)	100, 732, 1021, 2162
LmnI	GCTCC	3 cut(s)	315, 801, 2133
Lsp1109I	GCAGC	7 cut(s)	54, 777, 916, 1711, 1921, 1949, 2043
LweI	GCATC	5 cut(s)	260, 388, 667, 1329, 1792
MaeI	CTAG	6 cut(s)	18, 123, 402, 1025, 1560, 1575
MaeII	ACGT	8 cut(s)	133, 175, 916, 1536, 1618, 1708, 1863, 2177
MaeIII	GTNAC	5 cut(s)	668, 862, 1073, 1216, 1910
MalI	GATC	4 cut(s)	102, 734, 1023, 2164
MboI	GATC	4 cut(s)	100, 732, 1021, 2162
MboII	GAAGA	9 cut(s)	6, 80, 416, 614, 706, 1859, 2065, 2094, 2185
MflI	RGATCY	1 cut(s)	1021
MhlI	GDGCHC	2 cut(s)	320, 2130
MlsI	TGGCCA	1 cut(s)	1841
MluCI	AATT	8 cut(s)	25, 168, 265, 411, 1229, 1361, 1566, 1959
MluNI	TGGCCA	1 cut(s)	1841
MlyI	GAGTC	1 cut(s)	2117
MmeI	TCCRAC	1 cut(s)	732
Mox20I	TGGCCA	1 cut(s)	1841
Mph1103I	ATGCAT	2 cut(s)	682, 1089
MroI	TCCGGA	1 cut(s)	1904
MroXI	GAANNNNTTC	2 cut(s)	87, 207
MscI	TGGCCA	1 cut(s)	1841
MseI	TTAA	9 cut(s)	147, 972, 1017, 1107, 1206, 1482, 1539, 1674, 1916
MslI	CAYNNNNRTG	3 cut(s)	396, 1490, 2076
Msp20I	TGGCCA	1 cut(s)	1841
MspCI	CTTAAG	1 cut(s)	1205
MspI	CCGG	5 cut(s)	312, 430, 1421, 1905, 2018
MspR9I	CCNGG	6 cut(s)	180, 313, 875, 1421, 1422, 1629
Mva1269I	GAATGC	1 cut(s)	1170
MvaI	CCWGG	3 cut(s)	180, 875, 1629
MvnI	CGCG	2 cut(s)	322, 386
MwoI	GCNNNNNNNGC	2 cut(s)	1463, 1611
NciI	CCSGG	3 cut(s)	313, 1421, 1422
NdeII	GATC	4 cut(s)	100, 732, 1021, 2162
NlaIV	GGNNCC	3 cut(s)	317, 1300, 1417
NmuCI	GTSAC	3 cut(s)	862, 1073, 1910
NruI	TCGCGA	1 cut(s)	386
NsbI	TGCGCA	1 cut(s)	2076
NsiI	ATGCAT	2 cut(s)	682, 1089
NspI	RCATGY	2 cut(s)	1609, 2075
NspV	TTCGAA	1 cut(s)	201
PaqCI	CACCTGC	1 cut(s)	2074
PceI	AGGCCT	2 cut(s)	1357, 1738
PcsI	WCGNNNNNNNCGW	1 cut(s)	133
PctI	GAATGC	1 cut(s)	1170
PdmI	GAANNNNTTC	2 cut(s)	87, 207
PfeI	GAWTC	4 cut(s)	419, 653, 1401, 1921
PflFI	GACNNNGTC	1 cut(s)	132
PkrI	GCNGC	8 cut(s)	44, 601, 792, 906, 1726, 1936, 1939, 2033
PleI	GAGTC	1 cut(s)	2116
PpsI	GAGTC	1 cut(s)	2116
PshAI	GACNNNNGTC	1 cut(s)	2043
Psp124BI	GAGCTC	1 cut(s)	2130
Psp6I	CCWGG	3 cut(s)	178, 873, 1627
PspEI	GGTNACC	1 cut(s)	668
PspFI	CCCAGC	1 cut(s)	1062
PspGI	CCWGG	3 cut(s)	178, 873, 1627
PspN4I	GGNNCC	3 cut(s)	317, 1300, 1417
PspPI	GGNCC	3 cut(s)	338, 1299, 1415
PstI	CTGCAG	2 cut(s)	1939, 2033
PstNI	CAGNNNCTG	1 cut(s)	350
PsuI	RGATCY	1 cut(s)	1021
PsyI	GACNNNGTC	1 cut(s)	132
RruI	TCGCGA	1 cut(s)	386
RsaI	GTAC	7 cut(s)	498, 1426, 1535, 1693, 1719, 2016, 2027
RsaNI	GTAC	7 cut(s)	497, 1425, 1534, 1692, 1718, 2015, 2026
RseI	CAYNNNNRTG	3 cut(s)	396, 1490, 2076
SacI	GAGCTC	1 cut(s)	2130
SalI	GTCGAC	2 cut(s)	135, 2110
SaqAI	TTAA	9 cut(s)	147, 972, 1017, 1107, 1206, 1482, 1539, 1674, 1916
SatI	GCNGC	8 cut(s)	43, 600, 791, 905, 1725, 1935, 1938, 2032
Sau3AI	GATC	4 cut(s)	100, 732, 1021, 2162
Sau96I	GGNCC	3 cut(s)	338, 1299, 1415
SchI	GAGTC	1 cut(s)	2117
ScrFI	CCNGG	6 cut(s)	180, 313, 875, 1421, 1422, 1629
SduI	GDGCHC	2 cut(s)	320, 2130
SfaNI	GCATC	5 cut(s)	260, 388, 667, 1329, 1792
SfcI	CTRYAG	2 cut(s)	1935, 2029
SfuI	TTCGAA	1 cut(s)	201
SinI	GGWCC	2 cut(s)	338, 1415
SmaI	CCCGGG	1 cut(s)	1422
SmiMI	CAYNNNNRTG	3 cut(s)	396, 1490, 2076
SmlI	CTYRAG	3 cut(s)	491, 560, 1205
SmoI	CTYRAG	3 cut(s)	491, 560, 1205
Sse9I	AATT	8 cut(s)	25, 168, 265, 411, 1229, 1361, 1566, 1959
SseBI	AGGCCT	2 cut(s)	1357, 1738
SsiI	CCGC	4 cut(s)	320, 599, 1544, 2000
SspI	AATATT	1 cut(s)	293
SspMI	CTAG	6 cut(s)	18, 123, 402, 1025, 1560, 1575
SstI	GAGCTC	1 cut(s)	2130
StuI	AGGCCT	2 cut(s)	1357, 1738
StyD4I	CCNGG	6 cut(s)	178, 311, 873, 1419, 1420, 1627
StyI	CCWWGG	4 cut(s)	369, 778, 1302, 1371
TaaI	ACNGT	1 cut(s)	1380
TaiI	ACGT	8 cut(s)	136, 178, 919, 1539, 1621, 1711, 1866, 2180
TaqI	TCGA	4 cut(s)	136, 201, 938, 2111
TasI	AATT	8 cut(s)	25, 168, 265, 411, 1229, 1361, 1566, 1959
TatI	WGTACW	1 cut(s)	2025
TauI	GCSGC	1 cut(s)	602
TfiI	GAWTC	4 cut(s)	419, 653, 1401, 1921
Tru1I	TTAA	9 cut(s)	147, 972, 1017, 1107, 1206, 1482, 1539, 1674, 1916
Tru9I	TTAA	9 cut(s)	147, 972, 1017, 1107, 1206, 1482, 1539, 1674, 1916
TseFI	GTSAC	3 cut(s)	862, 1073, 1910
TseI	GCWGC	7 cut(s)	42, 790, 904, 1724, 1934, 1937, 2031
Tsp45I	GTSAC	3 cut(s)	862, 1073, 1910
TspDTI	ATGAA	6 cut(s)	288, 471, 839, 1242, 1657, 1670
TspMI	CCCGGG	1 cut(s)	1420
Tth111I	GACNNNGTC	1 cut(s)	132
Vha464I	CTTAAG	1 cut(s)	1205
VpaK11BI	GGWCC	2 cut(s)	338, 1415
XagI	CCTNNNNNAGG	2 cut(s)	1395, 1751
XapI	RAATTY	4 cut(s)	168, 265, 1229, 1959
XceI	RCATGY	2 cut(s)	1609, 2075
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1849
XmaI	CCCGGG	1 cut(s)	1420
XmiI	GTMKAC	2 cut(s)	136, 2111
XmnI	GAANNNNTTC	2 cut(s)	87, 207
XspI	CTAG	6 cut(s)	18, 123, 402, 1025, 1560, 1575
ZraI	GACGTC	1 cut(s)	2178
Zsp2I	ATGCAT	2 cut(s)	682, 1089

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.