Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0008867

Physical Location & Seq

Genomic Coordinates Reverse (-)

36975267 .. 36977012

Gene Structure 1746 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1292900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 783 bp

ATGGATGAAACCCTCTCTCCAGAACCCGACTCAGATGTTTCAAAGGAGCTAAGGCCAGAAACTCAGTCATCCAAAAGGAGGAAGGTGGCTCATGAGAAGACTGTTGTGACAGTGAAGATAGGAGCTAATGTTGGGAAGCTAAAGAATGAAGGGCCGCCTTTTGATCTTTGGTCATGGAGGAAATATGGCCAAAAACCCATCAAAGGATCTCCTTATCCAAGGGGATACTACCGTTGCAGTACATCGAAAGGTTGCTTGGCAAAAAAACAAGTGGAGAGAAGCAAAACTGATGCTTCAGTACTTATAATCACCTACACTTGTAGCCACAATCATTCAGGTCCTCCTGATTTGAATGTGATCTCCACCACCCAACAAGAACATGATCAAAAGCAGGAACAGGAACAAGAACAGGAACAAGAACAAGAACAAGAACAAGAACATGATCAAAAGCAAGATCAAAAGCAAGAACAAGAACAAGAACATCGAAAATTGGAAGAACACTTCCACTACATCGAGTCCCCAATCAGATTTTCGCAAGAAGAAGAACACATTTTTATTGCTCACGACACGAGTTTTGAGTTCCTATTGGATGAAAAACAAGAGCCACTATCACTATCATATTCTCAGATGATGAGCTTCTCAACACCCAAATTATCAGAAGAAAACAATGACTTCTTTGATGAGCTTGAAGAACTACCCACATTTTCATCCTTCCCAAGCTTCATGAGGAGGACTAACAATCTTTCCTTTGGACTTGAAAGGATTCCCTCTGTCCCTTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003700 GO:0006355 GO:0007275 GO:0008150 GO:0009555 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0010154 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0022414 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0048229 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0061458 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WRKY

Protein Analysis

261

Amino Acids

30.34

Weight (kDa)

5.17

Isoelectric Point (pI)

68.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRKY	PF03106	55 - 111	1.8e-24	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0008867)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g39060 FvH4_5g39060
malus_domestica	MD00G1140800.v1.1 MD03G1292900.v1.1
prunus_persica	Prupe.8G265900_v2.0.a1 Prupe.8G265900_v2.0.a1
pyrus_communis	pycom03g23220 pycom11g27360
rosa_chinensis	RchiOBHm_Chr7g0241021 RchiOBHm_Chr7g0241041
rosa_laevigata	RLG00000000603
rosa_multiflora	Rmu_sc0003187.1_g000008
rosa_roxburghii	Rroxscaffold_3G00219310
rosa_rugosa	Rorug07G0341300 Rorug07G0341400 Rorug07G0341500
rosa_samantha	Rh7AG496600 Rh7AG497100 Rh7BG467800 Rh7CG513800 Rh7DG481000
rosa_wichuraiana	Rw0G005810 Rw7G041940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	305
AciI	CCGC	1 cut(s)	155
AclWI	GGATC	1 cut(s)	214
AcoI	YGGCCR	1 cut(s)	187
AcuI	CTGAAG	1 cut(s)	279
AfaI	GTAC	2 cut(s)	241, 300
AfiI	CCNNNNNNNGG	2 cut(s)	78, 203
AgsI	TTSAA	4 cut(s)	42, 352, 689, 758
AjuI	GAANNNNNNNTTGG	4 cut(s)	239, 271, 514, 546
AluBI	AGCT	6 cut(s)	49, 125, 139, 636, 685, 720
AluI	AGCT	6 cut(s)	49, 125, 139, 636, 685, 720
AlwI	GGATC	1 cut(s)	214
AoxI	GGCC	3 cut(s)	53, 152, 187
ArsI	GACNNNNNNTTYG	2 cut(s)	557, 589
Asp700I	GAANNNNTTC	1 cut(s)	762
AspS9I	GGNCC	2 cut(s)	152, 338
AsuHPI	GGTGA	1 cut(s)	301
AvaII	GGWCC	1 cut(s)	338
BaeI	ACNNNNGTAYC	2 cut(s)	217, 250
BalI	TGGCCA	1 cut(s)	189
BauI	CACGAG	1 cut(s)	568
BbsI	GAAGAC	1 cut(s)	104
BccI	CCATC	1 cut(s)	206
BciVI	GTATCC	1 cut(s)	218
BclI	TGATCA	2 cut(s)	382, 442
BfuI	GTATCC	1 cut(s)	218
BisI	GCNGC	1 cut(s)	155
BlsI	GCNGC	1 cut(s)	156
BmcAI	AGTACT	1 cut(s)	300
Bme18I	GGWCC	1 cut(s)	338
BmgT120I	GGNCC	2 cut(s)	152, 338
BmsI	GCATC	1 cut(s)	280
BpiI	GAAGAC	1 cut(s)	104
Bpu10I	CCTNAGC	1 cut(s)	50
BsaJI	CCNNGG	1 cut(s)	218
BsaXI	ACNNNNNCTCC	3 cut(s)	31, 114, 144
Bsc4I	CCNNNNNNNGG	2 cut(s)	78, 203
BseDI	CCNNGG	1 cut(s)	218
BseGI	GGATG	4 cut(s)	10, 68, 595, 707
BseLI	CCNNNNNNNGG	2 cut(s)	78, 203
BseMII	CTCAG	3 cut(s)	45, 77, 638
BseRI	GAGGAG	1 cut(s)	742
BshFI	GGCC	3 cut(s)	55, 154, 189
BslFI	GGGAC	2 cut(s)	502, 758
BslI	CCNNNNNNNGG	2 cut(s)	78, 203
BsmFI	GGGAC	2 cut(s)	502, 758
BsnI	GGCC	3 cut(s)	55, 154, 189
Bsp143I	GATC	6 cut(s)	163, 206, 357, 382, 442, 454
BspACI	CCGC	1 cut(s)	155
BspANI	GGCC	3 cut(s)	55, 154, 189
BspCNI	CTCAG	3 cut(s)	44, 76, 637
BspHI	TCATGA	2 cut(s)	91, 723
BspPI	GGATC	1 cut(s)	214
BssECI	CCNNGG	1 cut(s)	218
BssMI	GATC	6 cut(s)	163, 206, 357, 382, 442, 454
BssSI	CACGAG	1 cut(s)	568
BssT1I	CCWWGG	1 cut(s)	218
Bst2BI	CACGAG	1 cut(s)	568
Bst4CI	ACNGT	3 cut(s)	103, 112, 233
BstDEI	CTNAG	4 cut(s)	31, 50, 63, 624
BstF5I	GGATG	4 cut(s)	10, 68, 595, 707
BstKTI	GATC	6 cut(s)	166, 209, 360, 385, 445, 457
BstMBI	GATC	6 cut(s)	163, 206, 357, 382, 442, 454
BstV2I	GAAGAC	1 cut(s)	104
BstX2I	RGATCY	1 cut(s)	206
BstYI	RGATCY	1 cut(s)	206
BsuI	GTATCC	1 cut(s)	218
BsuRI	GGCC	3 cut(s)	55, 154, 189
BtsCI	GGATG	4 cut(s)	10, 68, 595, 707
BtsIMutI	CAGTG	1 cut(s)	117
CciI	TCATGA	2 cut(s)	91, 723
Cfr13I	GGNCC	2 cut(s)	152, 338
Csp6I	GTAC	2 cut(s)	240, 299
CviAII	CATG	5 cut(s)	92, 174, 380, 440, 724
CviQI	GTAC	2 cut(s)	240, 299
DdeI	CTNAG	4 cut(s)	31, 50, 63, 624
DpnI	GATC	6 cut(s)	165, 208, 359, 384, 444, 456
DpnII	GATC	6 cut(s)	163, 206, 357, 382, 442, 454
EaeI	YGGCCR	1 cut(s)	187
Eco130I	CCWWGG	1 cut(s)	218
Eco47I	GGWCC	1 cut(s)	338
Eco57I	CTGAAG	1 cut(s)	279
EcoO109I	RGGNCCY	1 cut(s)	338
EcoT14I	CCWWGG	1 cut(s)	218
ErhI	CCWWGG	1 cut(s)	218
FaeI	CATG	5 cut(s)	95, 177, 383, 443, 727
FaiI	YATR	8 cut(s)	93, 175, 186, 305, 381, 441, 619, 725
FaqI	GGGAC	2 cut(s)	502, 758
FatI	CATG	5 cut(s)	91, 173, 379, 439, 723
FbaI	TGATCA	2 cut(s)	382, 442
Fnu4HI	GCNGC	1 cut(s)	155
FokI	GGATG	4 cut(s)	17, 55, 602, 694
Fsp4HI	GCNGC	1 cut(s)	155
GluI	GCNGC	1 cut(s)	155
HaeIII	GGCC	3 cut(s)	55, 154, 189
Hin1II	CATG	5 cut(s)	95, 177, 383, 443, 727
HindIII	AAGCTT	1 cut(s)	718
HinfI	GANTC	3 cut(s)	29, 515, 763
HphI	GGTGA	1 cut(s)	301
Hpy188I	TCNGA	4 cut(s)	34, 527, 627, 658
Hpy188III	TCNNGA	6 cut(s)	20, 92, 344, 563, 724, 780
HpyAV	CCTTC	3 cut(s)	76, 143, 721
HpyCH4III	ACNGT	3 cut(s)	103, 112, 233
HpyCH4V	TGCA	1 cut(s)	237
HpyF3I	CTNAG	4 cut(s)	31, 50, 63, 624
Hsp92II	CATG	5 cut(s)	95, 177, 383, 443, 727
Ksp22I	TGATCA	2 cut(s)	382, 442
Kzo9I	GATC	6 cut(s)	163, 206, 357, 382, 442, 454
LmnI	GCTCC	2 cut(s)	46, 122
LpnPI	CCDG	7 cut(s)	33, 69, 321, 357, 377, 383, 395
LweI	GCATC	1 cut(s)	280
MaeIII	GTNAC	1 cut(s)	106
MalI	GATC	6 cut(s)	165, 208, 359, 384, 444, 456
MboI	GATC	6 cut(s)	163, 206, 357, 382, 442, 454
MboII	GAAGA	7 cut(s)	109, 127, 506, 551, 554, 671, 701
MflI	RGATCY	1 cut(s)	206
MlsI	TGGCCA	1 cut(s)	189
MluCI	AATT	2 cut(s)	488, 650
MluNI	TGGCCA	1 cut(s)	189
MlyI	GAGTC	2 cut(s)	23, 524
MnlI	CCTC	7 cut(s)	23, 72, 171, 351, 720, 723, 778
Mox20I	TGGCCA	1 cut(s)	189
MroXI	GAANNNNTTC	1 cut(s)	762
MscI	TGGCCA	1 cut(s)	189
Msp20I	TGGCCA	1 cut(s)	189
NdeII	GATC	6 cut(s)	163, 206, 357, 382, 442, 454
NlaIII	CATG	5 cut(s)	95, 177, 383, 443, 727
NmuCI	GTSAC	1 cut(s)	106
PagI	TCATGA	2 cut(s)	91, 723
PdmI	GAANNNNTTC	1 cut(s)	762
PfeI	GAWTC	1 cut(s)	763
PkrI	GCNGC	1 cut(s)	156
PleI	GAGTC	2 cut(s)	23, 523
PpsI	GAGTC	2 cut(s)	23, 523
PpuMI	RGGWCCY	1 cut(s)	338
PsiI	TTATAA	1 cut(s)	305
Psp5II	RGGWCCY	1 cut(s)	338
PspPI	GGNCC	2 cut(s)	152, 338
PspPPI	RGGWCCY	1 cut(s)	338
PsuI	RGATCY	1 cut(s)	206
RsaI	GTAC	2 cut(s)	241, 300
RsaNI	GTAC	2 cut(s)	240, 299
SatI	GCNGC	1 cut(s)	155
Sau3AI	GATC	6 cut(s)	163, 206, 357, 382, 442, 454
Sau96I	GGNCC	2 cut(s)	152, 338
ScaI	AGTACT	1 cut(s)	300
SchI	GAGTC	2 cut(s)	23, 524
SfaNI	GCATC	1 cut(s)	280
SinI	GGWCC	1 cut(s)	338
Sse9I	AATT	2 cut(s)	488, 650
SsiI	CCGC	1 cut(s)	155
StyI	CCWWGG	1 cut(s)	218
TaaI	ACNGT	3 cut(s)	103, 112, 233
TaqI	TCGA	3 cut(s)	245, 484, 513
TasI	AATT	2 cut(s)	488, 650
TatI	WGTACW	2 cut(s)	239, 298
TauI	GCSGC	1 cut(s)	157
TfiI	GAWTC	1 cut(s)	763
TscAI	CASTG	1 cut(s)	117
TseFI	GTSAC	1 cut(s)	106
Tsp45I	GTSAC	1 cut(s)	106
TspDTI	ATGAA	5 cut(s)	21, 162, 606, 696, 712
TspRI	CASTG	1 cut(s)	117
VpaK11BI	GGWCC	1 cut(s)	338
XmnI	GAANNNNTTC	1 cut(s)	762
ZrmI	AGTACT	1 cut(s)	300

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.