Rh7AG497100
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0008867
Physical Location & Seq
Reverse (-)
69673172 .. 69673824
653 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG497100.1

Sequence Viewer

Length: 429 bp
ATGATTAGGCCAAAAACCCAGGCATCCAAAAGGAAGGTGGCTCATCAGAAGAATGAAGGGCCTCCTTCTGACTTCTGGTCATGGAGGAAATATGGGCAAAAACCTATCAAAGGATCTCCTTATCCAAGAGGGTACTACAGGTGCAGTACATCAAAGGGTTGTTCTGCCAAGAAACAAGTGGAGAGAAGCAAAACTGATGCTTCTGTGCTCATAATCACCTACACTAACAGCCATAACCATCGAGGTCCTCATGTGACCTCCACCACTAAAAACCAGAGCCCACCACCAAAAGAAGCCCAAATGCAACCCAATAGTGAGGATGATGATCATGATCTTACAGCTGAAGTGCCCAAAGAGGATGAACCAGAGCCTGAGCCAGATCAAGACGAAGAACAAGAACAAGAAAGGAAGGTAGATGAATATGAATAG

Protein Analysis

142

Amino Acids

16.32

Weight (kDa)

6.23

Isoelectric Point (pI)

43.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 24 - 80 5.8e-26 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 121
AcuI CTGAAG 1 cut(s) 363
AfaI GTAC 2 cut(s) 134, 148
AfiI CCNNNNNNNGG 1 cut(s) 110
AhdI GACNNNNNGTC 1 cut(s) 76
AjnI CCWGG 1 cut(s) 18
AluBI AGCT 1 cut(s) 341
AluI AGCT 1 cut(s) 341
Alw21I GWGCWC 1 cut(s) 210
AlwI GGATC 1 cut(s) 121
AlwNI CAGNNNCTG 1 cut(s) 371
AoxI GGCC 2 cut(s) 8, 59
AspS9I GGNCC 2 cut(s) 59, 245
AsuHPI GGTGA 1 cut(s) 208
AvaII GGWCC 1 cut(s) 245
BaeGI GKGCMC 1 cut(s) 351
BanII GRGCYC 1 cut(s) 281
Bbv12I GWGCWC 1 cut(s) 210
BccI CCATC 1 cut(s) 246
BciT130I CCWGG 1 cut(s) 20
BclI TGATCA 1 cut(s) 325
BfmI CTRYAG 1 cut(s) 136
Bme1390I CCNGG 1 cut(s) 20
Bme18I GGWCC 1 cut(s) 245
BmeRI GACNNNNNGTC 1 cut(s) 76
BmgT120I GGNCC 2 cut(s) 59, 245
BmrFI CCNGG 1 cut(s) 20
BmsI GCATC 2 cut(s) 32, 187
Bpu10I CCTNAGC 1 cut(s) 372
BsaBI GATNNNNATC 2 cut(s) 324, 330
BsaJI CCNNGG 1 cut(s) 18
Bsc4I CCNNNNNNNGG 1 cut(s) 110
Bse8I GATNNNNATC 2 cut(s) 324, 330
BseBI CCWGG 1 cut(s) 20
BseDI CCNNGG 1 cut(s) 18
BseGI GGATG 3 cut(s) 23, 325, 364
BseJI GATNNNNATC 2 cut(s) 324, 330
BseLI CCNNNNNNNGG 1 cut(s) 110
BseMII CTCAG 1 cut(s) 363
BseSI GKGCMC 1 cut(s) 351
BsgI GTGCAG 1 cut(s) 163
BshFI GGCC 2 cut(s) 10, 61
BsiHKAI GWGCWC 1 cut(s) 210
BslI CCNNNNNNNGG 1 cut(s) 110
BsnI GGCC 2 cut(s) 10, 61
Bsp1286I GDGCHC 3 cut(s) 210, 281, 351
Bsp143I GATC 4 cut(s) 113, 325, 331, 379
BspANI GGCC 2 cut(s) 10, 61
BspCNI CTCAG 1 cut(s) 364
BspHI TCATGA 1 cut(s) 328
BspPI GGATC 1 cut(s) 121
BssECI CCNNGG 1 cut(s) 18
BssMI GATC 4 cut(s) 113, 325, 331, 379
Bst2UI CCWGG 1 cut(s) 20
BstDEI CTNAG 1 cut(s) 372
BstENI CCTNNNNNAGG 1 cut(s) 108
BstF5I GGATG 3 cut(s) 23, 325, 364
BstKTI GATC 4 cut(s) 116, 328, 334, 382
BstMBI GATC 4 cut(s) 113, 325, 331, 379
BstNI CCWGG 1 cut(s) 20
BstSCI CCNGG 1 cut(s) 18
BstSFI CTRYAG 1 cut(s) 136
BstSLI GKGCMC 1 cut(s) 351
BstX2I RGATCY 1 cut(s) 113
BstYI RGATCY 1 cut(s) 113
BsuRI GGCC 2 cut(s) 10, 61
BtsCI GGATG 3 cut(s) 23, 325, 364
CaiI CAGNNNCTG 1 cut(s) 371
CciI TCATGA 1 cut(s) 328
Cfr13I GGNCC 2 cut(s) 59, 245
Csp6I GTAC 2 cut(s) 133, 147
CviAII CATG 3 cut(s) 81, 251, 329
CviJI RGCY 9 cut(s) 10, 41, 61, 231, 279, 296, 341, 370, 376
CviKI_1 RGCY 9 cut(s) 10, 41, 61, 231, 279, 296, 341, 370, 376
CviQI GTAC 2 cut(s) 133, 147
DdeI CTNAG 1 cut(s) 372
DpnI GATC 4 cut(s) 115, 327, 333, 381
DpnII GATC 4 cut(s) 113, 325, 331, 379
DriI GACNNNNNGTC 1 cut(s) 76
Eam1105I GACNNNNNGTC 1 cut(s) 76
Eco24I GRGCYC 1 cut(s) 281
Eco47I GGWCC 1 cut(s) 245
Eco57I CTGAAG 1 cut(s) 363
EcoNI CCTNNNNNAGG 1 cut(s) 108
EcoO109I RGGNCCY 2 cut(s) 59, 245
EcoRII CCWGG 1 cut(s) 18
EcoT38I GRGCYC 1 cut(s) 281
FaeI CATG 3 cut(s) 84, 254, 332
FaiI YATR 7 cut(s) 82, 93, 212, 234, 252, 330, 423
FatI CATG 3 cut(s) 80, 250, 328
FbaI TGATCA 1 cut(s) 325
FokI GGATG 3 cut(s) 10, 332, 371
FriOI GRGCYC 1 cut(s) 281
HaeIII GGCC 2 cut(s) 10, 61
Hin1II CATG 3 cut(s) 84, 254, 332
HphI GGTGA 1 cut(s) 208
Hpy188I TCNGA 2 cut(s) 48, 70
Hpy188III TCNNGA 2 cut(s) 329, 383
HpyAV CCTTC 4 cut(s) 28, 50, 75, 403
HpyCH4V TGCA 2 cut(s) 144, 304
HpyF3I CTNAG 1 cut(s) 372
Hsp92II CATG 3 cut(s) 84, 254, 332
Ksp22I TGATCA 1 cut(s) 325
Kzo9I GATC 4 cut(s) 113, 325, 331, 379
LpnPI CCDG 8 cut(s) 5, 32, 61, 124, 287, 378, 384, 390
LweI GCATC 2 cut(s) 32, 187
MaeIII GTNAC 1 cut(s) 253
MalI GATC 4 cut(s) 115, 327, 333, 381
MboI GATC 4 cut(s) 113, 325, 331, 379
MboII GAAGA 2 cut(s) 61, 401
MflI RGATCY 1 cut(s) 113
MhlI GDGCHC 3 cut(s) 210, 281, 351
MnlI CCTC 8 cut(s) 72, 78, 122, 236, 258, 268, 310, 349
MspA1I CMGCKG 1 cut(s) 341
MspR9I CCNGG 1 cut(s) 20
MvaI CCWGG 1 cut(s) 20
NdeII GATC 4 cut(s) 113, 325, 331, 379
NlaIII CATG 3 cut(s) 84, 254, 332
NmuCI GTSAC 1 cut(s) 253
PagI TCATGA 1 cut(s) 328
PpuMI RGGWCCY 1 cut(s) 245
Psp5II RGGWCCY 1 cut(s) 245
Psp6I CCWGG 1 cut(s) 18
PspGI CCWGG 1 cut(s) 18
PspPI GGNCC 2 cut(s) 59, 245
PspPPI RGGWCCY 1 cut(s) 245
PstNI CAGNNNCTG 1 cut(s) 371
PsuI RGATCY 1 cut(s) 113
PvuII CAGCTG 1 cut(s) 341
RsaI GTAC 2 cut(s) 134, 148
RsaNI GTAC 2 cut(s) 133, 147
Sau3AI GATC 4 cut(s) 113, 325, 331, 379
Sau96I GGNCC 2 cut(s) 59, 245
ScrFI CCNGG 1 cut(s) 20
SduI GDGCHC 3 cut(s) 210, 281, 351
SetI ASST 8 cut(s) 39, 106, 143, 221, 247, 260, 343, 414
SfaNI GCATC 2 cut(s) 32, 187
SfcI CTRYAG 1 cut(s) 136
SinI GGWCC 1 cut(s) 245
StyD4I CCNGG 1 cut(s) 18
TaqI TCGA 1 cut(s) 241
TatI WGTACW 1 cut(s) 146
TseFI GTSAC 1 cut(s) 253
Tsp45I GTSAC 1 cut(s) 253
TspDTI ATGAA 2 cut(s) 69, 375
VpaK11BI GGWCC 1 cut(s) 245
XagI CCTNNNNNAGG 1 cut(s) 108
XcmI CCANNNNNNNNNTGG 2 cut(s) 34, 175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.