MD03G1298400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0014518
Physical Location & Seq
Forward (+)
37393866 .. 37394907
1042 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1298400.v1.1.491

Sequence Viewer

Length: 858 bp
ATGCAATATTATTATTCAAGACTATCGTTGGGTGCAGCTTCTGTAGCAAAAAGCAGGTCGATTTGGGGCCATGGTCCTCATCAATTAGCCGCAGCTCGAGTAGTACTACTACGAGGACTTGTTTCTGCCACCGCTAGTCGCACCGGCACTGCTGACCCAGCCATTCACGCTGGTGGGGACTATGAATCTGACCCTGGTCTCACCACCGCAGACCAAGGTACAGAAGATGTTGCACAGGAGAAACCAGCTGTTGTTGAGCAGCGCGGCGCCAGCGGCAGAGGAAAAGGAACAGAACCTCTTGCACCTCCTAATAAACCCTCGTATACAAACGCGAGTTCCCCAAGGCTAGAGAACACAGGAGTGAACCTGCAGCCCTCGGACCACCCAACCAATTATACTCAGAAGAGACATATAAGTAGCCACGGAAAAGCTGCTACTAGTTTGTTGGAAAAGGTGAGCTGTGTGGGGCTGGACGGCACACCATTGTCAATTGACTCATCAAATGAAATGGACACGCAGGAAAAAGAGCAGTCGATGAGAGATAGAGATAGAGACCGGAAGGAGGAGCGTGAAGAGGATGATAGAGAGTACTACAAGCATCACAAGGCCTCACCACTGTCAGAGGTTGAGTATGCAGACACCCGAAAGCCCATTACGCGTGCAACAGACGGCACGGCAGACTCTGTGTCGGAGTCTGGTGCAGGTAGAGATGTCATTGGATGGAGGCCCGAGCAGTTGGACACGGCTGAGGAGGCACTCCGTAGAGCTGCAAGGATATGGAAAGAGAATGCCATCCGTGGGGACCCTGATGCTCCTCAGTCCAGGGTGCTCAGAGCTTTACGTGGGGAGTCATTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

286

Amino Acids

31.08

Weight (kDa)

5.95

Isoelectric Point (pI)

43.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014518)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02700 AT4G02140 AT4G02140
fragaria_vesca FvH4_5g39680
malus_domestica MD03G1298400.v1.1
prunus_persica Prupe.8G268800_v2.0.a1
pyrus_communis pycom03g23640
rosa_chinensis RchiOBHm_Chr7g0241981
rosa_laevigata RLG00000000524
rosa_multiflora Rmu_sc0003266.1_g000004
rosa_roxburghii Rroxscaffold_3G00218400
rosa_rugosa Rorug07G0349500
rosa_samantha Rh7AG503500 Rh7BG474700 Rh7CG520300 Rh7DG489100
rosa_wichuraiana Rw7G042580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 45, 375, 692
AccB1I GGYRCC 1 cut(s) 266
AccI GTMKAC 1 cut(s) 323
AccII CGCG 3 cut(s) 264, 332, 658
AciI CCGC 5 cut(s) 90, 132, 207, 264, 273
AcyI GRCGYC 1 cut(s) 267
AfaI GTAC 3 cut(s) 105, 220, 590
AfiI CCNNNNNNNGG 2 cut(s) 562, 798
AflIII ACRYGT 1 cut(s) 656
AgsI TTSAA 1 cut(s) 18
AhdI GACNNNNNGTC 1 cut(s) 685
AhlI ACTAGT 1 cut(s) 437
AjnI CCWGG 2 cut(s) 193, 821
AleI CACNNNNGTG 2 cut(s) 171, 359
AluBI AGCT 7 cut(s) 38, 95, 248, 431, 459, 767, 836
AluI AGCT 7 cut(s) 38, 95, 248, 431, 459, 767, 836
Alw21I GWGCWC 1 cut(s) 831
Alw26I GTCTC 3 cut(s) 203, 400, 546
AlwNI CAGNNNCTG 2 cut(s) 41, 683
Ama87I CYCGRG 2 cut(s) 96, 728
AoxI GGCC 3 cut(s) 67, 606, 725
ApeKI GCWGC 6 cut(s) 35, 92, 259, 370, 431, 767
ArsI GACNNNNNNTTYG 2 cut(s) 41, 73
AspLEI GCGC 2 cut(s) 264, 269
AspS9I GGNCC 5 cut(s) 67, 74, 379, 726, 802
AsuHPI GGTGA 3 cut(s) 193, 466, 603
AvaI CYCGRG 2 cut(s) 96, 728
AvaII GGWCC 3 cut(s) 74, 379, 802
BanI GGYRCC 1 cut(s) 266
Bbv12I GWGCWC 1 cut(s) 831
BbvCI CCTCAGC 1 cut(s) 747
BbvI GCAGC 6 cut(s) 47, 104, 271, 382, 418, 754
BccI CCATC 2 cut(s) 714, 800
BceAI ACGGC 4 cut(s) 490, 685, 690, 759
BciT130I CCWGG 2 cut(s) 195, 823
BcoDI GTCTC 3 cut(s) 203, 400, 546
BcuI ACTAGT 1 cut(s) 437
BfaI CTAG 3 cut(s) 135, 347, 438
BfmI CTRYAG 2 cut(s) 42, 368
BfoI RGCGCY 1 cut(s) 270
BfuAI ACCTGC 3 cut(s) 45, 375, 692
BisI GCNGC 9 cut(s) 36, 90, 93, 260, 265, 274, 371, 432, 768
BlsI GCNGC 9 cut(s) 37, 91, 94, 261, 266, 275, 372, 433, 769
BmcAI AGTACT 2 cut(s) 105, 590
Bme1390I CCNGG 2 cut(s) 195, 823
Bme18I GGWCC 3 cut(s) 74, 379, 802
BmeRI GACNNNNNGTC 1 cut(s) 685
BmeT110I CYCGRG 2 cut(s) 96, 728
BmgT120I GGNCC 5 cut(s) 67, 74, 379, 726, 802
BmiI GGNNCC 4 cut(s) 68, 268, 803, 804
BmrFI CCNGG 2 cut(s) 195, 823
BmsI GCATC 2 cut(s) 607, 799
BoxI GACNNNNGTC 1 cut(s) 195
Bpu10I CCTNAGC 1 cut(s) 747
BsaAI YACGTR 1 cut(s) 842
BsaHI GRCGYC 1 cut(s) 267
BsaI GGTCTC 2 cut(s) 203, 546
BsaJI CCNNGG 8 cut(s) 70, 193, 214, 341, 375, 421, 796, 822
BsaWI WCCGGW 1 cut(s) 555
Bsc4I CCNNNNNNNGG 2 cut(s) 562, 798
Bse118I RCCGGY 1 cut(s) 143
BseBI CCWGG 2 cut(s) 195, 823
BseDI CCNNGG 8 cut(s) 70, 193, 214, 341, 375, 421, 796, 822
BseGI GGATG 3 cut(s) 583, 725, 792
BseLI CCNNNNNNNGG 2 cut(s) 562, 798
BseMII CTCAG 4 cut(s) 413, 738, 830, 844
BseRI GAGGAG 3 cut(s) 578, 764, 804
BseXI GCAGC 6 cut(s) 47, 104, 271, 382, 418, 754
BseYI CCCAGC 1 cut(s) 157
BsgI GTGCAG 2 cut(s) 54, 720
Bsh1236I CGCG 3 cut(s) 264, 332, 658
BshFI GGCC 3 cut(s) 69, 608, 727
BshNI GGYRCC 1 cut(s) 266
BsiHKAI GWGCWC 1 cut(s) 831
BsiHKCI CYCGRG 2 cut(s) 96, 728
BsiSI CCGG 2 cut(s) 144, 556
BslFI GGGAC 2 cut(s) 191, 815
BslI CCNNNNNNNGG 2 cut(s) 562, 798
BsmAI GTCTC 3 cut(s) 203, 400, 546
BsmFI GGGAC 2 cut(s) 191, 815
BsmI GAATGC 1 cut(s) 793
BsnI GGCC 3 cut(s) 69, 608, 727
Bso31I GGTCTC 2 cut(s) 203, 546
BsoBI CYCGRG 2 cut(s) 96, 728
Bsp1286I GDGCHC 1 cut(s) 831
Bsp19I CCATGG 1 cut(s) 70
BspACI CCGC 5 cut(s) 90, 132, 207, 264, 273
BspANI GGCC 3 cut(s) 69, 608, 727
BspCNI CTCAG 4 cut(s) 412, 739, 829, 843
BspFNI CGCG 3 cut(s) 264, 332, 658
BspLI GGNNCC 4 cut(s) 68, 268, 803, 804
BspMAI CTGCAG 1 cut(s) 372
BspMI ACCTGC 3 cut(s) 45, 375, 692
BspT107I GGYRCC 1 cut(s) 266
BspTNI GGTCTC 2 cut(s) 203, 546
BsrFI RCCGGY 1 cut(s) 143
BssAI RCCGGY 1 cut(s) 143
BssECI CCNNGG 8 cut(s) 70, 193, 214, 341, 375, 421, 796, 822
BssNAI GTATAC 1 cut(s) 324
BssNI GRCGYC 1 cut(s) 267
BssT1I CCWWGG 3 cut(s) 70, 214, 341
Bst1107I GTATAC 1 cut(s) 324
Bst2UI CCWGG 2 cut(s) 195, 823
Bst4CI ACNGT 1 cut(s) 618
Bst6I CTCTTC 2 cut(s) 398, 567
BstACI GRCGYC 1 cut(s) 267
BstBAI YACGTR 1 cut(s) 842
BstC8I GCNNGC 2 cut(s) 271, 660
BstDEI CTNAG 4 cut(s) 399, 747, 816, 830
BstDSI CCRYGG 3 cut(s) 70, 421, 796
BstF5I GGATG 3 cut(s) 583, 725, 792
BstFNI CGCG 3 cut(s) 264, 332, 658
BstH2I RGCGCY 1 cut(s) 270
BstHHI GCGC 2 cut(s) 264, 269
BstMAI GTCTC 3 cut(s) 203, 400, 546
BstMWI GCNNNNNNNGC 7 cut(s) 44, 158, 167, 270, 273, 655, 752
BstNI CCWGG 2 cut(s) 195, 823
BstPAI GACNNNNGTC 1 cut(s) 195
BstSCI CCNGG 2 cut(s) 193, 821
BstSFI CTRYAG 2 cut(s) 42, 368
BstUI CGCG 3 cut(s) 264, 332, 658
BstV1I GCAGC 6 cut(s) 47, 104, 271, 382, 418, 754
BstZ17I GTATAC 1 cut(s) 324
BsuRI GGCC 3 cut(s) 69, 608, 727
BtgI CCRYGG 3 cut(s) 70, 421, 796
BtsCI GGATG 3 cut(s) 583, 725, 792
BtsI GCAGTG 1 cut(s) 147
BtsIMutI CAGTG 2 cut(s) 147, 614
BveI ACCTGC 3 cut(s) 45, 375, 692
Cac8I GCNNGC 2 cut(s) 271, 660
CaiI CAGNNNCTG 2 cut(s) 41, 683
CfoI GCGC 2 cut(s) 264, 269
Cfr10I RCCGGY 1 cut(s) 143
Cfr13I GGNCC 5 cut(s) 67, 74, 379, 726, 802
Csp6I GTAC 3 cut(s) 104, 219, 589
CviAII CATG 1 cut(s) 71
CviQI GTAC 3 cut(s) 104, 219, 589
DdeI CTNAG 4 cut(s) 399, 747, 816, 830
DinI GGCGCC 1 cut(s) 268
DriI GACNNNNNGTC 1 cut(s) 685
Eam1104I CTCTTC 2 cut(s) 398, 567
Eam1105I GACNNNNNGTC 1 cut(s) 685
EarI CTCTTC 2 cut(s) 398, 567
Eco130I CCWWGG 3 cut(s) 70, 214, 341
Eco147I AGGCCT 1 cut(s) 608
Eco31I GGTCTC 2 cut(s) 203, 546
Eco47I GGWCC 3 cut(s) 74, 379, 802
Eco88I CYCGRG 2 cut(s) 96, 728
EcoO109I RGGNCCY 1 cut(s) 802
EcoRII CCWGG 2 cut(s) 193, 821
EcoT14I CCWWGG 3 cut(s) 70, 214, 341
EgeI GGCGCC 1 cut(s) 268
EheI GGCGCC 1 cut(s) 268
ErhI CCWWGG 3 cut(s) 70, 214, 341
FaeI CATG 1 cut(s) 74
FaiI YATR 8 cut(s) 72, 183, 324, 396, 411, 413, 633, 778
FaqI GGGAC 2 cut(s) 191, 815
FatI CATG 1 cut(s) 70
FblI GTMKAC 1 cut(s) 323
Fnu4HI GCNGC 9 cut(s) 36, 90, 93, 260, 265, 274, 371, 432, 768
FokI GGATG 3 cut(s) 590, 732, 779
Fsp4HI GCNGC 9 cut(s) 36, 90, 93, 260, 265, 274, 371, 432, 768
FspBI CTAG 3 cut(s) 135, 347, 438
GlaI GCGC 2 cut(s) 263, 268
GluI GCNGC 9 cut(s) 36, 90, 93, 260, 265, 274, 371, 432, 768
GsaI CCCAGC 1 cut(s) 161
HaeII RGCGCY 1 cut(s) 270
HaeIII GGCC 3 cut(s) 69, 608, 727
HapII CCGG 2 cut(s) 144, 556
HhaI GCGC 2 cut(s) 264, 269
Hin1I GRCGYC 1 cut(s) 267
Hin1II CATG 1 cut(s) 74
Hin6I GCGC 2 cut(s) 262, 267
HinP1I GCGC 2 cut(s) 262, 267
HinfI GANTC 5 cut(s) 185, 494, 680, 692, 848
HpaII CCGG 2 cut(s) 144, 556
HphI GGTGA 3 cut(s) 193, 466, 603
Hpy166II GTNNAC 2 cut(s) 324, 364
Hpy188I TCNGA 6 cut(s) 190, 379, 402, 622, 691, 833
Hpy188III TCNNGA 1 cut(s) 18
Hpy8I GTNNAC 2 cut(s) 324, 364
HpyAV CCTTC 1 cut(s) 553
HpyCH4III ACNGT 1 cut(s) 618
HpyCH4IV ACGT 1 cut(s) 841
HpyCH4V TGCA 9 cut(s) 4, 35, 233, 302, 370, 635, 662, 701, 770
HpyF10VI GCNNNNNNNGC 7 cut(s) 44, 158, 167, 270, 273, 655, 752
HpyF3I CTNAG 4 cut(s) 399, 747, 816, 830
HpySE526I ACGT 1 cut(s) 841
Hsp92I GRCGYC 1 cut(s) 267
Hsp92II CATG 1 cut(s) 74
HspAI GCGC 2 cut(s) 262, 267
KasI GGCGCC 1 cut(s) 266
KflI GGGWCCC 1 cut(s) 802
LmnI GCTCC 2 cut(s) 565, 817
Lsp1109I GCAGC 6 cut(s) 47, 104, 271, 382, 418, 754
LweI GCATC 2 cut(s) 607, 799
MaeI CTAG 3 cut(s) 135, 347, 438
MaeII ACGT 1 cut(s) 841
MboII GAAGA 3 cut(s) 236, 415, 584
MfeI CAATTG 1 cut(s) 489
MhlI GDGCHC 1 cut(s) 831
MluCI AATT 3 cut(s) 83, 391, 489
MluI ACGCGT 1 cut(s) 656
Mly113I GGCGCC 1 cut(s) 267
MlyI GAGTC 4 cut(s) 488, 674, 701, 857
MmeI TCCRAC 3 cut(s) 426, 669, 717
MslI CAYNNNNRTG 2 cut(s) 171, 359
MspA1I CMGCKG 2 cut(s) 248, 273
MspI CCGG 2 cut(s) 144, 556
MspR9I CCNGG 2 cut(s) 195, 823
MteI GCGCNGCGC 1 cut(s) 265
MunI CAATTG 1 cut(s) 489
Mva1269I GAATGC 1 cut(s) 793
MvaI CCWGG 2 cut(s) 195, 823
MvnI CGCG 3 cut(s) 264, 332, 658
MwoI GCNNNNNNNGC 7 cut(s) 44, 158, 167, 270, 273, 655, 752
NarI GGCGCC 1 cut(s) 267
NcoI CCATGG 1 cut(s) 70
NlaIII CATG 1 cut(s) 74
NlaIV GGNNCC 4 cut(s) 68, 268, 803, 804
OliI CACNNNNGTG 2 cut(s) 171, 359
PaeR7I CTCGAG 1 cut(s) 96
PceI AGGCCT 1 cut(s) 608
PctI GAATGC 1 cut(s) 793
PfeI GAWTC 1 cut(s) 185
PkrI GCNGC 9 cut(s) 37, 91, 94, 261, 266, 275, 372, 433, 769
PleI GAGTC 4 cut(s) 488, 674, 700, 856
PluTI GGCGCC 1 cut(s) 270
PpsI GAGTC 4 cut(s) 488, 674, 700, 856
Ppu21I YACGTR 1 cut(s) 842
PpuMI RGGWCCY 1 cut(s) 802
PshAI GACNNNNGTC 1 cut(s) 195
Psp5II RGGWCCY 1 cut(s) 802
Psp6I CCWGG 2 cut(s) 193, 821
PspFI CCCAGC 1 cut(s) 157
PspGI CCWGG 2 cut(s) 193, 821
PspN4I GGNNCC 4 cut(s) 68, 268, 803, 804
PspPI GGNCC 5 cut(s) 67, 74, 379, 726, 802
PspPPI RGGWCCY 1 cut(s) 802
PspXI VCTCGAGB 1 cut(s) 96
PstI CTGCAG 1 cut(s) 372
PstNI CAGNNNCTG 2 cut(s) 41, 683
PvuII CAGCTG 1 cut(s) 248
RsaI GTAC 3 cut(s) 105, 220, 590
RsaNI GTAC 3 cut(s) 104, 219, 589
RseI CAYNNNNRTG 2 cut(s) 171, 359
SatI GCNGC 9 cut(s) 36, 90, 93, 260, 265, 274, 371, 432, 768
Sau96I GGNCC 5 cut(s) 67, 74, 379, 726, 802
ScaI AGTACT 2 cut(s) 105, 590
SchI GAGTC 4 cut(s) 488, 674, 701, 857
ScrFI CCNGG 2 cut(s) 195, 823
SduI GDGCHC 1 cut(s) 831
SfaNI GCATC 2 cut(s) 607, 799
SfcI CTRYAG 2 cut(s) 42, 368
SfoI GGCGCC 1 cut(s) 268
Sfr274I CTCGAG 1 cut(s) 96
SinI GGWCC 3 cut(s) 74, 379, 802
SlaI CTCGAG 1 cut(s) 96
SmiMI CAYNNNNRTG 2 cut(s) 171, 359
SmlI CTYRAG 1 cut(s) 96
SmoI CTYRAG 1 cut(s) 96
SpeI ACTAGT 1 cut(s) 437
Sse9I AATT 3 cut(s) 83, 391, 489
SseBI AGGCCT 1 cut(s) 608
SsiI CCGC 5 cut(s) 90, 132, 207, 264, 273
SspDI GGCGCC 1 cut(s) 266
SspI AATATT 1 cut(s) 8
SspMI CTAG 3 cut(s) 135, 347, 438
StuI AGGCCT 1 cut(s) 608
StyD4I CCNGG 2 cut(s) 193, 821
StyI CCWWGG 3 cut(s) 70, 214, 341
TaaI ACNGT 1 cut(s) 618
TaiI ACGT 1 cut(s) 844
TaqI TCGA 3 cut(s) 59, 97, 533
TasI AATT 3 cut(s) 83, 391, 489
TatI WGTACW 2 cut(s) 103, 588
TauI GCSGC 3 cut(s) 92, 267, 276
TfiI GAWTC 1 cut(s) 185
TscAI CASTG 2 cut(s) 154, 621
TseI GCWGC 6 cut(s) 35, 92, 259, 370, 431, 767
TspDTI ATGAA 2 cut(s) 198, 519
TspGWI ACGGA 3 cut(s) 438, 749, 785
TspRI CASTG 2 cut(s) 154, 621
VpaK11BI GGWCC 3 cut(s) 74, 379, 802
XhoI CTCGAG 1 cut(s) 96
XmiI GTMKAC 1 cut(s) 323
XspI CTAG 3 cut(s) 135, 347, 438
ZrmI AGTACT 2 cut(s) 105, 590
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.