Rroxscaffold_3G00218400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0014518
Physical Location & Seq
Reverse (-)
600420 .. 601416
997 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00218400.1

Sequence Viewer

Length: 807 bp
ATGCAATGGAGAATATTATCAGTAGCTGCAGCTATATATAAAGTACGAAGGGCGTCGCGTATTTGGGTCTTGACTAGTATTCAAGCTGATCTTCGGAGAGGACTTGCCTCGGCCAGATCTGGTCGTACTGCGGACCCTGCCGTTCACGCTGGGGACTTTGACGCTGACCCTGTTGTCACTACAGGAGATCCGGAAGCTCCAGAAAATGTTACAAAGGAGAAATTGGTTGATGAAGAACATATATATACAGAAGACAGCCGTAGATGCAGAGGAACAGAGCCACTGTCACCACCTAAACCTCCATATGCTGGTTCCCCCAGGCTAGAGAGCACAGGGGTAAACCAGCTGCCTTTGGATCCAACCTTTCAGCAGAAGCGTAGGCTTGCAACCGTAGGTGGGGCTAATTCATTAGAAGACTTGAGCTGTGCAGGGCTTGACGGCTCACCATGGCCAAAGGACACGGAAAAACAGCAGACAGACAGGGAAGAACAACAAGAGGGTGAACAAGAGTACTACAAGCATCATCAGGCGTCACCCTTGTCAGAGATTAAGGTGGCCGATACGCGAAAGCCCATTACCCGCGCCACAGACAGGACAGCCGACGCTAGGGAAGAATATGGTGCAGGTAGAGAGGTGATTGGATGGAGGCCTGAGCAGCTGGATACAGCCGAGGAGGCACTGCTTAGAGCTGCAAGGATATGGAAGGAGAACGCCATGCGTGGTGACCCTGATGCTCCTCAATCTAGGGTTCTCAGACTACTACTGCGTGAGCGTGGAGATTCAACTACAGAATATGTGATCGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

268

Amino Acids

29.97

Weight (kDa)

5.37

Isoelectric Point (pI)

56.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014518)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02700 AT4G02140 AT4G02140
fragaria_vesca FvH4_5g39680
malus_domestica MD03G1298400.v1.1
prunus_persica Prupe.8G268800_v2.0.a1
pyrus_communis pycom03g23640
rosa_chinensis RchiOBHm_Chr7g0241981
rosa_laevigata RLG00000000524
rosa_multiflora Rmu_sc0003266.1_g000004
rosa_roxburghii Rroxscaffold_3G00218400
rosa_rugosa Rorug07G0349500
rosa_samantha Rh7AG503500 Rh7BG474700 Rh7CG520300 Rh7DG489100
rosa_wichuraiana Rw7G042580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 173
Acc36I ACCTGC 1 cut(s) 614
AccB7I CCANNNNNTGG 1 cut(s) 308
AccII CGCG 3 cut(s) 58, 565, 582
AccIII TCCGGA 1 cut(s) 190
AciI CCGC 2 cut(s) 131, 580
AclWI GGATC 3 cut(s) 182, 350, 363
AcoI YGGCCR 3 cut(s) 111, 449, 555
AcyI GRCGYC 2 cut(s) 53, 530
AfaI GTAC 3 cut(s) 45, 127, 512
AfiI CCNNNNNNNGG 4 cut(s) 308, 396, 591, 606
AgsI TTSAA 2 cut(s) 83, 783
AhlI ACTAGT 1 cut(s) 74
AjnI CCWGG 1 cut(s) 317
AluBI AGCT 8 cut(s) 26, 32, 86, 197, 346, 423, 658, 689
AluI AGCT 8 cut(s) 26, 32, 86, 197, 346, 423, 658, 689
Alw21I GWGCWC 1 cut(s) 332
AlwI GGATC 3 cut(s) 182, 350, 363
AlwNI CAGNNNCTG 1 cut(s) 26
Aor13HI TCCGGA 1 cut(s) 190
AoxI GGCC 4 cut(s) 111, 449, 555, 647
ApeKI GCWGC 5 cut(s) 26, 29, 346, 655, 689
AspLEI GCGC 1 cut(s) 584
AspS9I GGNCC 1 cut(s) 133
AsuHPI GGTGA 6 cut(s) 279, 435, 512, 525, 646, 734
AvaII GGWCC 1 cut(s) 133
BalI TGGCCA 1 cut(s) 451
BamHI GGATCC 1 cut(s) 355
BbsI GAAGAC 2 cut(s) 258, 420
Bbv12I GWGCWC 1 cut(s) 332
BbvI GCAGC 5 cut(s) 13, 41, 333, 667, 676
BccI CCATC 1 cut(s) 636
BceAI ACGGC 3 cut(s) 125, 243, 454
BciT130I CCWGG 1 cut(s) 319
BciVI GTATCC 1 cut(s) 655
BcuI ACTAGT 1 cut(s) 74
BfaI CTAG 4 cut(s) 75, 323, 606, 744
BfmI CTRYAG 3 cut(s) 27, 180, 786
BfuAI ACCTGC 1 cut(s) 614
BfuI GTATCC 1 cut(s) 655
BglI GCCNNNNNGGC 1 cut(s) 674
BglII AGATCT 1 cut(s) 116
BisI GCNGC 5 cut(s) 27, 30, 347, 656, 690
BlsI GCNGC 5 cut(s) 28, 31, 348, 657, 691
BmcAI AGTACT 1 cut(s) 512
Bme1390I CCNGG 1 cut(s) 319
Bme18I GGWCC 1 cut(s) 133
BmgT120I GGNCC 1 cut(s) 133
BmiI GGNNCC 3 cut(s) 135, 313, 357
BmrFI CCNGG 1 cut(s) 319
BmsI GCATC 3 cut(s) 254, 529, 721
BpiI GAAGAC 2 cut(s) 258, 420
BpmI CTGGAG 1 cut(s) 183
Bpu10I CCTNAGC 1 cut(s) 651
BpuEI CTTGAG 1 cut(s) 439
Bsa29I ATCGAT 1 cut(s) 801
BsaHI GRCGYC 2 cut(s) 53, 530
BsaJI CCNNGG 4 cut(s) 108, 317, 446, 669
BsaWI WCCGGW 1 cut(s) 190
Bsc4I CCNNNNNNNGG 4 cut(s) 308, 396, 591, 606
Bse3DI GCAATG 1 cut(s) 11
BseAI TCCGGA 1 cut(s) 190
BseBI CCWGG 1 cut(s) 319
BseCI ATCGAT 1 cut(s) 801
BseDI CCNNGG 4 cut(s) 108, 317, 446, 669
BseGI GGATG 1 cut(s) 647
BseLI CCNNNNNNNGG 4 cut(s) 308, 396, 591, 606
BseMI GCAATG 1 cut(s) 11
BseMII CTCAG 2 cut(s) 642, 766
BseRI GAGGAG 2 cut(s) 686, 726
BseXI GCAGC 5 cut(s) 13, 41, 333, 667, 676
BseYI CCCAGC 1 cut(s) 149
BsgI GTGCAG 2 cut(s) 447, 642
Bsh1236I CGCG 3 cut(s) 58, 565, 582
BshFI GGCC 4 cut(s) 113, 451, 557, 649
BshVI ATCGAT 1 cut(s) 801
BsiHKAI GWGCWC 1 cut(s) 332
BsiSI CCGG 1 cut(s) 191
BslFI GGGAC 1 cut(s) 167
BslI CCNNNNNNNGG 4 cut(s) 308, 396, 591, 606
BsmFI GGGAC 1 cut(s) 167
BsnI GGCC 4 cut(s) 113, 451, 557, 649
Bsp1286I GDGCHC 1 cut(s) 332
Bsp13I TCCGGA 1 cut(s) 190
Bsp143I GATC 5 cut(s) 88, 116, 187, 355, 798
Bsp19I CCATGG 1 cut(s) 446
BspACI CCGC 2 cut(s) 131, 580
BspANI GGCC 4 cut(s) 113, 451, 557, 649
BspCNI CTCAG 2 cut(s) 643, 765
BspDI ATCGAT 1 cut(s) 801
BspEI TCCGGA 1 cut(s) 190
BspFNI CGCG 3 cut(s) 58, 565, 582
BspLI GGNNCC 3 cut(s) 135, 313, 357
BspMAI CTGCAG 1 cut(s) 31
BspMI ACCTGC 1 cut(s) 614
BspPI GGATC 3 cut(s) 182, 350, 363
BsrDI GCAATG 1 cut(s) 11
BssECI CCNNGG 4 cut(s) 108, 317, 446, 669
BssMI GATC 5 cut(s) 88, 116, 187, 355, 798
BssNI GRCGYC 2 cut(s) 53, 530
BssT1I CCWWGG 1 cut(s) 446
Bst2UI CCWGG 1 cut(s) 319
Bst4CI ACNGT 2 cut(s) 285, 391
BstACI GRCGYC 2 cut(s) 53, 530
BstC8I GCNNGC 1 cut(s) 384
BstDEI CTNAG 3 cut(s) 651, 683, 752
BstDSI CCRYGG 1 cut(s) 446
BstEII GGTNACC 1 cut(s) 722
BstF5I GGATG 1 cut(s) 647
BstFNI CGCG 3 cut(s) 58, 565, 582
BstHHI GCGC 1 cut(s) 584
BstKTI GATC 5 cut(s) 91, 119, 190, 358, 801
BstMBI GATC 5 cut(s) 88, 116, 187, 355, 798
BstMWI GCNNNNNNNGC 5 cut(s) 137, 146, 264, 655, 674
BstNI CCWGG 1 cut(s) 319
BstPI GGTNACC 1 cut(s) 722
BstSCI CCNGG 1 cut(s) 317
BstSFI CTRYAG 3 cut(s) 27, 180, 786
BstUI CGCG 3 cut(s) 58, 565, 582
BstV1I GCAGC 5 cut(s) 13, 41, 333, 667, 676
BstV2I GAAGAC 2 cut(s) 258, 420
BstX2I RGATCY 3 cut(s) 116, 187, 355
BstYI RGATCY 3 cut(s) 116, 187, 355
Bsu15I ATCGAT 1 cut(s) 801
BsuI GTATCC 1 cut(s) 655
BsuRI GGCC 4 cut(s) 113, 451, 557, 649
BsuTUI ATCGAT 1 cut(s) 801
BtgI CCRYGG 1 cut(s) 446
BtsCI GGATG 1 cut(s) 647
BtsI GCAGTG 1 cut(s) 677
BtsIMutI CAGTG 2 cut(s) 281, 677
BveI ACCTGC 1 cut(s) 614
Cac8I GCNNGC 1 cut(s) 384
CaiI CAGNNNCTG 1 cut(s) 26
CfoI GCGC 1 cut(s) 584
Cfr13I GGNCC 1 cut(s) 133
ClaI ATCGAT 1 cut(s) 801
CseI GACGC 4 cut(s) 42, 170, 519, 611
Csp6I GTAC 3 cut(s) 44, 126, 511
CviAII CATG 2 cut(s) 447, 715
CviQI GTAC 3 cut(s) 44, 126, 511
DdeI CTNAG 3 cut(s) 651, 683, 752
DpnI GATC 5 cut(s) 90, 118, 189, 357, 800
DpnII GATC 5 cut(s) 88, 116, 187, 355, 798
DrdI GACNNNNNNGTC 1 cut(s) 173
DseDI GACNNNNNNGTC 1 cut(s) 173
EaeI YGGCCR 3 cut(s) 111, 449, 555
Eco130I CCWWGG 1 cut(s) 446
Eco147I AGGCCT 1 cut(s) 649
Eco47I GGWCC 1 cut(s) 133
Eco91I GGTNACC 1 cut(s) 722
EcoO65I GGTNACC 1 cut(s) 722
EcoRII CCWGG 1 cut(s) 317
EcoT14I CCWWGG 1 cut(s) 446
ErhI CCWWGG 1 cut(s) 446
FaeI CATG 2 cut(s) 450, 718
FalI AAGNNNNNCTT 2 cut(s) 75, 107
FaqI GGGAC 1 cut(s) 167
FatI CATG 2 cut(s) 446, 714
FauI CCCGC 1 cut(s) 587
FauNDI CATATG 1 cut(s) 304
Fnu4HI GCNGC 5 cut(s) 27, 30, 347, 656, 690
FokI GGATG 1 cut(s) 654
Fsp4HI GCNGC 5 cut(s) 27, 30, 347, 656, 690
FspBI CTAG 4 cut(s) 75, 323, 606, 744
GlaI GCGC 1 cut(s) 583
GluI GCNGC 5 cut(s) 27, 30, 347, 656, 690
GsaI CCCAGC 1 cut(s) 153
GsuI CTGGAG 1 cut(s) 183
HaeIII GGCC 4 cut(s) 113, 451, 557, 649
HapII CCGG 1 cut(s) 191
HgaI GACGC 4 cut(s) 42, 170, 519, 611
HhaI GCGC 1 cut(s) 584
Hin1I GRCGYC 2 cut(s) 53, 530
Hin1II CATG 2 cut(s) 450, 718
Hin6I GCGC 1 cut(s) 582
HinP1I GCGC 1 cut(s) 582
HinfI GANTC 1 cut(s) 779
HpaII CCGG 1 cut(s) 191
HphI GGTGA 6 cut(s) 279, 435, 512, 525, 646, 734
Hpy166II GTNNAC 3 cut(s) 145, 340, 503
Hpy188I TCNGA 3 cut(s) 96, 544, 755
Hpy188III TCNNGA 3 cut(s) 70, 191, 200
Hpy8I GTNNAC 3 cut(s) 145, 340, 503
Hpy99I CGWCG 2 cut(s) 58, 605
HpyAV CCTTC 2 cut(s) 42, 697
HpyCH4III ACNGT 2 cut(s) 285, 391
HpyCH4V TGCA 7 cut(s) 4, 29, 267, 386, 428, 623, 692
HpyF10VI GCNNNNNNNGC 5 cut(s) 137, 146, 264, 655, 674
HpyF3I CTNAG 3 cut(s) 651, 683, 752
Hsp92I GRCGYC 2 cut(s) 53, 530
Hsp92II CATG 2 cut(s) 450, 718
HspAI GCGC 1 cut(s) 582
Kpn2I TCCGGA 1 cut(s) 190
Kzo9I GATC 5 cut(s) 88, 116, 187, 355, 798
LmnI GCTCC 2 cut(s) 202, 739
Lsp1109I GCAGC 5 cut(s) 13, 41, 333, 667, 676
LweI GCATC 3 cut(s) 254, 529, 721
MaeI CTAG 4 cut(s) 75, 323, 606, 744
MaeIII GTNAC 5 cut(s) 175, 208, 285, 531, 722
MalI GATC 5 cut(s) 90, 118, 189, 357, 800
MboI GATC 5 cut(s) 88, 116, 187, 355, 798
MboII GAAGA 6 cut(s) 83, 245, 263, 425, 497, 623
MflI RGATCY 3 cut(s) 116, 187, 355
MhlI GDGCHC 1 cut(s) 332
MlsI TGGCCA 1 cut(s) 451
MluCI AATT 2 cut(s) 221, 403
MluNI TGGCCA 1 cut(s) 451
MmeI TCCRAC 1 cut(s) 383
Mox20I TGGCCA 1 cut(s) 451
MroI TCCGGA 1 cut(s) 190
MscI TGGCCA 1 cut(s) 451
MseI TTAA 2 cut(s) 549, 805
Msp20I TGGCCA 1 cut(s) 451
MspA1I CMGCKG 2 cut(s) 346, 658
MspI CCGG 1 cut(s) 191
MspR9I CCNGG 1 cut(s) 319
MvaI CCWGG 1 cut(s) 319
MvnI CGCG 3 cut(s) 58, 565, 582
MwoI GCNNNNNNNGC 5 cut(s) 137, 146, 264, 655, 674
NcoI CCATGG 1 cut(s) 446
NdeI CATATG 1 cut(s) 304
NdeII GATC 5 cut(s) 88, 116, 187, 355, 798
NlaIII CATG 2 cut(s) 450, 718
NlaIV GGNNCC 3 cut(s) 135, 313, 357
NmeAIII GCCGAG 2 cut(s) 89, 694
NmuCI GTSAC 4 cut(s) 175, 285, 531, 722
PceI AGGCCT 1 cut(s) 649
PfeI GAWTC 1 cut(s) 779
PflMI CCANNNNNTGG 1 cut(s) 308
PkrI GCNGC 5 cut(s) 28, 31, 348, 657, 691
Psp6I CCWGG 1 cut(s) 317
PspEI GGTNACC 1 cut(s) 722
PspFI CCCAGC 1 cut(s) 149
PspGI CCWGG 1 cut(s) 317
PspN4I GGNNCC 3 cut(s) 135, 313, 357
PspPI GGNCC 1 cut(s) 133
PstI CTGCAG 1 cut(s) 31
PstNI CAGNNNCTG 1 cut(s) 26
PsuI RGATCY 3 cut(s) 116, 187, 355
PvuII CAGCTG 2 cut(s) 346, 658
RsaI GTAC 3 cut(s) 45, 127, 512
RsaNI GTAC 3 cut(s) 44, 126, 511
SaqAI TTAA 2 cut(s) 549, 805
SatI GCNGC 5 cut(s) 27, 30, 347, 656, 690
Sau3AI GATC 5 cut(s) 88, 116, 187, 355, 798
Sau96I GGNCC 1 cut(s) 133
ScaI AGTACT 1 cut(s) 512
ScrFI CCNGG 1 cut(s) 319
SduI GDGCHC 1 cut(s) 332
SfaNI GCATC 3 cut(s) 254, 529, 721
SfcI CTRYAG 3 cut(s) 27, 180, 786
SinI GGWCC 1 cut(s) 133
SmlI CTYRAG 1 cut(s) 418
SmoI CTYRAG 1 cut(s) 418
SpeI ACTAGT 1 cut(s) 74
Sse9I AATT 2 cut(s) 221, 403
SseBI AGGCCT 1 cut(s) 649
SsiI CCGC 2 cut(s) 131, 580
SspI AATATT 1 cut(s) 15
SspMI CTAG 4 cut(s) 75, 323, 606, 744
StuI AGGCCT 1 cut(s) 649
StyD4I CCNGG 1 cut(s) 317
StyI CCWWGG 1 cut(s) 446
TaaI ACNGT 2 cut(s) 285, 391
TaqI TCGA 1 cut(s) 801
TasI AATT 2 cut(s) 221, 403
TatI WGTACW 1 cut(s) 510
TfiI GAWTC 1 cut(s) 779
Tru1I TTAA 2 cut(s) 549, 805
Tru9I TTAA 2 cut(s) 549, 805
TscAI CASTG 2 cut(s) 288, 684
TseFI GTSAC 4 cut(s) 175, 285, 531, 722
TseI GCWGC 5 cut(s) 26, 29, 346, 655, 689
Tsp45I GTSAC 4 cut(s) 175, 285, 531, 722
TspDTI ATGAA 2 cut(s) 246, 396
TspGWI ACGGA 1 cut(s) 476
TspRI CASTG 2 cut(s) 288, 684
Van91I CCANNNNNTGG 1 cut(s) 308
VpaK11BI GGWCC 1 cut(s) 133
XspI CTAG 4 cut(s) 75, 323, 606, 744
ZrmI AGTACT 1 cut(s) 512
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.