Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0008857

Physical Location & Seq

Genomic Coordinates Forward (+)

674017 .. 677904

Gene Structure 3888 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1005700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1320 bp

ATGGAAGACGATAAAACCCTAATCAAACAACAGCAACAAGAACCACAGGGGGAGCTCCAAGACGAGGAATTTCGAAAAGAGTTTGAATGCTGCATTTGCCTGGATCTTCTTTACAAGCCAGTTGTATTAGGTTGTGGCCACATTTCGTGTTTCTGGTGTGTCTTTAAGACCATGAGTGCTTTTCATGAGTCTTATTGTCCCGTTTGTCGGCACCCATTTAATCATTTTCCAAGTATCTGTCAGTTGATGCATTTCTTGCTCCAGAAGTTGTATCCTGAAGCATATGAGAGAAGGGGAAAACAAGTGGAAGAAGAAAAGGAAGTTGGTTATTTCTCACCTCAATTTGATAATCCACTATCTGGGTCACATCGTATTGAGGAGTCAAATATTCTGGGCAATGTTCACTCAAAGAACTTGGAAAGTTGTTCTTCTGGGGTAGTACAATCTTCCTCACTTGTAAATACTTCAGGAACTATGTTAGATTATGAAGTTAATATTACAAGCCCTGCAACATCTCCAAAGAACGTTGAAGCTACTGGAAATGCAGCCATCCAAGAGGACTGTACGCGTGATATTGATATTGGAAATGGAACTCACCAGAAGGTTTCTGTTTCTGATCTGCTGTGTGCTGCATGCGAGAACCTGCTCTTTCAACCTGTTGTTCTCAATTGCGGTCATGTATTTTGTGAGTCCTGTATCAACATTCCAGCTGATGGAATTTCTAGGTGTCAGATTTGTCAAAGCATGCATCCAAAGGGATTCCCTAGTGTTTGCAAAATTTTAGAGCATTTCTTGGAGGAGCAATACCCTGAAGTTTATTTGCAACGACGAGTAACTTCAATAAAATTGCGTGATCATCAACGTGCTGGCCAGTCATCGTCGACACTTTCTGATGATTACTTATCTTGCTGTGCTGGGCAGAAAGTTCATTACGGAGTTGGTTGTGATTGCTGTGGGATATGTCCAATTATTGGGGAGAGGTACAGATGCAAAGACTGCGTGGAGAAAATAGGTTTTGACCTATGTGGAGCATGCAACAATGCGCCTTTTAATATCACCGGTCGATTTAATCAGCAACATAAACCAGAACACAAGCTTGAGCTTGTACCTCCAGGTGTTCTCTATCGGATTGATGACGATGGTGCCCTGTTTCAGGAAGACCTAGGAATCGTCACTGCTGCTCCCATTCCAGAAGATCCAGAAAATGGCCCTAGCGATGTGCAAAATGTTTCTCCTGCTTTTGTATTCTCAGCTGATAGCTCAGTAGATCCGCATGATGATTTGGATGGCAGTGCTTTATCAGACTCAATGCAAGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ZZ zf-C3HC4_3 zf-C3HC4_4 zf-RING_UBOX

Protein Analysis

440

Amino Acids

48.77

Weight (kDa)

4.85

Isoelectric Point (pI)

59.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-C3HC4_3	PF13920	27 - 74	7.7e-07	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_4	PF15227	30 - 69	5.9e-06	zinc finger of C3HC4-type, RING

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0008857)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g00570 FvH4_4g00570
malus_domestica	MD04G1005700.v1.1
prunus_persica	Prupe.1G006100_v2.0.a1 Prupe.1G006100_v2.0.a1 Prupe.1G006100_v2.0.a1 Prupe.1G006100_v2.0.a1
pyrus_communis	pycom420g00860
rosa_chinensis	RchiOBHm_Chr4g0386021
rosa_laevigata	RLG00000010180
rosa_multiflora	Rmu_sc0000472.1_g000019
rosa_roxburghii	Rroxscaffold_5G00333780 Rroxscaffold_5G00338450 Rroxscaffold_5G00338750
rosa_rugosa	Rorug03G0306300 Rorug03G0306400 Rorug03G0306400 Rorug03G0306500
rosa_samantha	Rh4AG010100 Rh4BG007100 Rh4CG010800 Rh4DG008200
rosa_wichuraiana	Rw4G000130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	651
AccB1I	GGYRCC	2 cut(s)	210, 1142
AccB7I	CCANNNNNTGG	4 cut(s)	359, 713, 971, 1205
AccI	GTMKAC	1 cut(s)	881
AccII	CGCG	1 cut(s)	568
AciI	CCGC	2 cut(s)	672, 1271
AclI	AACGTT	1 cut(s)	525
AclWI	GGATC	3 cut(s)	111, 1190, 1262
AcoI	YGGCCR	2 cut(s)	136, 868
AcsI	RAATTY	3 cut(s)	68, 717, 777
AcuI	CTGAAG	3 cut(s)	297, 450, 831
AfaI	GTAC	4 cut(s)	441, 565, 983, 1107
AfiI	CCNNNNNNNGG	7 cut(s)	64, 207, 359, 713, 971, 1153, 1205
AflIII	ACRYGT	1 cut(s)	566
AgeI	ACCGGT	1 cut(s)	1058
AgsI	TTSAA	4 cut(s)	86, 530, 653, 840
AjnI	CCWGG	2 cut(s)	99, 1111
AjuI	GAANNNNNNNTTGG	2 cut(s)	306, 338
AluBI	AGCT	7 cut(s)	55, 533, 710, 1096, 1102, 1253, 1260
AluI	AGCT	7 cut(s)	55, 533, 710, 1096, 1102, 1253, 1260
Alw21I	GWGCWC	1 cut(s)	57
AlwI	GGATC	3 cut(s)	111, 1190, 1262
AoxI	GGCC	3 cut(s)	136, 868, 1207
ApeKI	GCWGC	4 cut(s)	90, 545, 629, 1178
ApoI	RAATTY	3 cut(s)	68, 717, 777
AsiGI	ACCGGT	1 cut(s)	1058
AspA2I	CCTAGG	1 cut(s)	1162
AspLEI	GCGC	1 cut(s)	1045
AspS9I	GGNCC	1 cut(s)	1208
AsuHPI	GGTGA	3 cut(s)	327, 587, 1048
AsuII	TTCGAA	1 cut(s)	73
AvrII	CCTAGG	1 cut(s)	1162
BaeGI	GKGCMC	1 cut(s)	1147
BalI	TGGCCA	2 cut(s)	138, 870
BanI	GGYRCC	2 cut(s)	210, 1142
BanII	GRGCYC	1 cut(s)	57
BbsI	GAAGAC	2 cut(s)	12, 1164
Bbv12I	GWGCWC	1 cut(s)	57
BbvI	GCAGC	4 cut(s)	77, 557, 616, 1165
BccI	CCATC	4 cut(s)	557, 707, 1133, 1280
BciT130I	CCWGG	2 cut(s)	101, 1113
BciVI	GTATCC	1 cut(s)	282
BclI	TGATCA	1 cut(s)	853
BfaI	CTAG	4 cut(s)	723, 765, 1163, 1212
BfuAI	ACCTGC	1 cut(s)	651
BfuI	GTATCC	1 cut(s)	282
BisI	GCNGC	4 cut(s)	91, 546, 630, 1179
BlnI	CCTAGG	1 cut(s)	1162
BlsI	GCNGC	4 cut(s)	92, 547, 631, 1180
Bme1390I	CCNGG	2 cut(s)	101, 1113
BmgT120I	GGNCC	1 cut(s)	1208
BmiI	GGNNCC	2 cut(s)	212, 1144
BmrFI	CCNGG	2 cut(s)	101, 1113
BmsI	GCATC	3 cut(s)	237, 757, 977
BpiI	GAAGAC	2 cut(s)	12, 1164
BpmI	CTGGAG	2 cut(s)	245, 1095
Bpu14I	TTCGAA	1 cut(s)	73
BpuEI	CTTGAG	1 cut(s)	1118
BsaJI	CCNNGG	1 cut(s)	1162
BsaWI	WCCGGW	1 cut(s)	1058
BsaXI	ACNNNNNCTCC	2 cut(s)	1165, 1195
Bsc4I	CCNNNNNNNGG	7 cut(s)	64, 207, 359, 713, 971, 1153, 1205
Bse118I	RCCGGY	1 cut(s)	1058
Bse1I	ACTGG	3 cut(s)	119, 541, 871
Bse3DI	GCAATG	1 cut(s)	403
BseBI	CCWGG	2 cut(s)	101, 1113
BseDI	CCNNGG	1 cut(s)	1162
BseGI	GGATG	3 cut(s)	549, 748, 1291
BseLI	CCNNNNNNNGG	7 cut(s)	64, 207, 359, 713, 971, 1153, 1205
BseMI	GCAATG	1 cut(s)	403
BseMII	CTCAG	2 cut(s)	1263, 1275
BseNI	ACTGG	3 cut(s)	119, 541, 871
BseRI	GAGGAG	2 cut(s)	392, 812
BseSI	GKGCMC	1 cut(s)	1147
BseXI	GCAGC	4 cut(s)	77, 557, 616, 1165
BseYI	CCCAGC	1 cut(s)	914
Bsh1236I	CGCG	1 cut(s)	568
Bsh1285I	CGRYCG	1 cut(s)	1063
BshFI	GGCC	3 cut(s)	138, 870, 1209
BshNI	GGYRCC	2 cut(s)	210, 1142
BshTI	ACCGGT	1 cut(s)	1058
BsiEI	CGRYCG	1 cut(s)	1063
BsiHKAI	GWGCWC	1 cut(s)	57
BsiSI	CCGG	1 cut(s)	1059
BslFI	GGGAC	1 cut(s)	183
BslI	CCNNNNNNNGG	7 cut(s)	64, 207, 359, 713, 971, 1153, 1205
BsmFI	GGGAC	1 cut(s)	183
BsmI	GAATGC	1 cut(s)	92
BsnI	GGCC	3 cut(s)	138, 870, 1209
Bsp119I	TTCGAA	1 cut(s)	73
Bsp1286I	GDGCHC	2 cut(s)	57, 1147
Bsp143I	GATC	5 cut(s)	103, 616, 853, 1195, 1267
BspACI	CCGC	2 cut(s)	672, 1271
BspANI	GGCC	3 cut(s)	138, 870, 1209
BspCNI	CTCAG	2 cut(s)	1262, 1274
BspFNI	CGCG	1 cut(s)	568
BspHI	TCATGA	1 cut(s)	184
BspLI	GGNNCC	2 cut(s)	212, 1144
BspMI	ACCTGC	1 cut(s)	651
BspPI	GGATC	3 cut(s)	111, 1190, 1262
BspT104I	TTCGAA	1 cut(s)	73
BspT107I	GGYRCC	2 cut(s)	210, 1142
BsrDI	GCAATG	1 cut(s)	403
BsrFI	RCCGGY	1 cut(s)	1058
BsrI	ACTGG	3 cut(s)	119, 541, 871
BssAI	RCCGGY	1 cut(s)	1058
BssECI	CCNNGG	1 cut(s)	1162
BssMI	GATC	5 cut(s)	103, 616, 853, 1195, 1267
BssT1I	CCWWGG	1 cut(s)	1162
Bst2UI	CCWGG	2 cut(s)	101, 1113
Bst4CI	ACNGT	1 cut(s)	563
BstAPI	GCANNNNNTGC	2 cut(s)	256, 996
BstBI	TTCGAA	1 cut(s)	73
BstC8I	GCNNGC	4 cut(s)	634, 746, 868, 1033
BstDEI	CTNAG	2 cut(s)	1249, 1261
BstENI	CCTNNNNNAGG	1 cut(s)	1151
BstF5I	GGATG	3 cut(s)	549, 748, 1291
BstFNI	CGCG	1 cut(s)	568
BstHHI	GCGC	1 cut(s)	1045
BstKTI	GATC	5 cut(s)	106, 619, 856, 1198, 1270
BstMBI	GATC	5 cut(s)	103, 616, 853, 1195, 1267
BstMCI	CGRYCG	1 cut(s)	1063
BstMWI	GCNNNNNNNGC	3 cut(s)	96, 256, 996
BstNI	CCWGG	2 cut(s)	101, 1113
BstNSI	RCATGY	3 cut(s)	636, 748, 1035
BstSCI	CCNGG	2 cut(s)	99, 1111
BstSLI	GKGCMC	1 cut(s)	1147
BstUI	CGCG	1 cut(s)	568
BstV1I	GCAGC	4 cut(s)	77, 557, 616, 1165
BstV2I	GAAGAC	2 cut(s)	12, 1164
BstX2I	RGATCY	3 cut(s)	103, 1195, 1267
BstYI	RGATCY	3 cut(s)	103, 1195, 1267
BsuI	GTATCC	1 cut(s)	282
BsuRI	GGCC	3 cut(s)	138, 870, 1209
BtgZI	GCGATG	1 cut(s)	1230
BtsCI	GGATG	3 cut(s)	549, 748, 1291
BtsI	GCAGTG	2 cut(s)	1173, 1297
BtsIMutI	CAGTG	2 cut(s)	1173, 1297
BveI	ACCTGC	1 cut(s)	651
Cac8I	GCNNGC	4 cut(s)	634, 746, 868, 1033
CciI	TCATGA	1 cut(s)	184
CfoI	GCGC	1 cut(s)	1045
Cfr10I	RCCGGY	1 cut(s)	1058
Cfr13I	GGNCC	1 cut(s)	1208
Csp6I	GTAC	4 cut(s)	440, 564, 982, 1106
CspAI	ACCGGT	1 cut(s)	1058
CviAII	CATG	7 cut(s)	172, 185, 633, 677, 745, 1032, 1274
CviQI	GTAC	4 cut(s)	440, 564, 982, 1106
DdeI	CTNAG	2 cut(s)	1249, 1261
DpnI	GATC	5 cut(s)	105, 618, 855, 1197, 1269
DpnII	GATC	5 cut(s)	103, 616, 853, 1195, 1267
EaeI	YGGCCR	2 cut(s)	136, 868
Ecl136II	GAGCTC	1 cut(s)	55
Eco130I	CCWWGG	1 cut(s)	1162
Eco24I	GRGCYC	1 cut(s)	57
Eco53kI	GAGCTC	1 cut(s)	55
Eco57I	CTGAAG	3 cut(s)	297, 450, 831
EcoICRI	GAGCTC	1 cut(s)	55
EcoNI	CCTNNNNNAGG	1 cut(s)	1151
EcoRII	CCWGG	2 cut(s)	99, 1111
EcoT14I	CCWWGG	1 cut(s)	1162
EcoT22I	ATGCAT	2 cut(s)	252, 750
EcoT38I	GRGCYC	1 cut(s)	57
ErhI	CCWWGG	1 cut(s)	1162
FaeI	CATG	7 cut(s)	175, 188, 636, 680, 748, 1035, 1277
FalI	AAGNNNNNCTT	2 cut(s)	412, 444
FaqI	GGGAC	1 cut(s)	183
FatI	CATG	7 cut(s)	171, 184, 632, 676, 744, 1031, 1273
FauNDI	CATATG	1 cut(s)	283
FbaI	TGATCA	1 cut(s)	853
FblI	GTMKAC	1 cut(s)	881
Fnu4HI	GCNGC	4 cut(s)	91, 546, 630, 1179
FokI	GGATG	3 cut(s)	536, 735, 1298
FriOI	GRGCYC	1 cut(s)	57
Fsp4HI	GCNGC	4 cut(s)	91, 546, 630, 1179
FspBI	CTAG	4 cut(s)	723, 765, 1163, 1212
GlaI	GCGC	1 cut(s)	1044
GluI	GCNGC	4 cut(s)	91, 546, 630, 1179
GsaI	CCCAGC	1 cut(s)	918
GsuI	CTGGAG	2 cut(s)	245, 1095
HaeIII	GGCC	3 cut(s)	138, 870, 1209
HapII	CCGG	1 cut(s)	1059
HhaI	GCGC	1 cut(s)	1045
Hin1II	CATG	7 cut(s)	175, 188, 636, 680, 748, 1035, 1277
Hin6I	GCGC	1 cut(s)	1043
HinP1I	GCGC	1 cut(s)	1043
HincII	GTYRAC	1 cut(s)	882
HindII	GTYRAC	1 cut(s)	882
HindIII	AAGCTT	1 cut(s)	1094
HinfI	GANTC	6 cut(s)	188, 380, 689, 759, 1167, 1304
HpaII	CCGG	1 cut(s)	1059
HphI	GGTGA	3 cut(s)	327, 587, 1048
Hpy166II	GTNNAC	2 cut(s)	403, 882
Hpy188I	TCNGA	5 cut(s)	616, 732, 892, 1128, 1303
Hpy188III	TCNNGA	7 cut(s)	185, 262, 275, 468, 1154, 1190, 1199
Hpy8I	GTNNAC	2 cut(s)	403, 882
Hpy99I	CGWCG	2 cut(s)	831, 883
HpyAV	CCTTC	2 cut(s)	285, 595
HpyCH4III	ACNGT	1 cut(s)	563
HpyCH4IV	ACGT	2 cut(s)	525, 862
HpyF10VI	GCNNNNNNNGC	3 cut(s)	96, 256, 996
HpyF3I	CTNAG	2 cut(s)	1249, 1261
HpySE526I	ACGT	2 cut(s)	525, 862
Hsp92II	CATG	7 cut(s)	175, 188, 636, 680, 748, 1035, 1277
HspAI	GCGC	1 cut(s)	1043
Ksp22I	TGATCA	1 cut(s)	853
Kzo9I	GATC	5 cut(s)	103, 616, 853, 1195, 1267
LmnI	GCTCC	6 cut(s)	52, 60, 264, 799, 1028, 1186
Lsp1109I	GCAGC	4 cut(s)	77, 557, 616, 1165
LweI	GCATC	3 cut(s)	237, 757, 977
MaeI	CTAG	4 cut(s)	723, 765, 1163, 1212
MaeII	ACGT	2 cut(s)	525, 862
MaeIII	GTNAC	3 cut(s)	363, 832, 1171
MalI	GATC	5 cut(s)	105, 618, 855, 1197, 1269
MboI	GATC	5 cut(s)	103, 616, 853, 1195, 1267
MboII	GAAGA	8 cut(s)	17, 98, 320, 323, 420, 438, 1169, 1205
MfeI	CAATTG	1 cut(s)	667
MflI	RGATCY	3 cut(s)	103, 1195, 1267
MhlI	GDGCHC	2 cut(s)	57, 1147
MlsI	TGGCCA	2 cut(s)	138, 870
MluCI	AATT	7 cut(s)	68, 341, 667, 717, 777, 845, 966
MluI	ACGCGT	1 cut(s)	566
MluNI	TGGCCA	2 cut(s)	138, 870
MlyI	GAGTC	4 cut(s)	197, 389, 698, 1298
MnlI	CCTC	8 cut(s)	58, 348, 370, 460, 550, 790, 972, 1119
Mox20I	TGGCCA	2 cut(s)	138, 870
Mph1103I	ATGCAT	2 cut(s)	252, 750
MscI	TGGCCA	2 cut(s)	138, 870
MseI	TTAA	5 cut(s)	165, 219, 492, 1050, 1068
MslI	CAYNNNNRTG	1 cut(s)	861
Msp20I	TGGCCA	2 cut(s)	138, 870
MspA1I	CMGCKG	2 cut(s)	710, 1253
MspI	CCGG	1 cut(s)	1059
MspR9I	CCNGG	2 cut(s)	101, 1113
MunI	CAATTG	1 cut(s)	667
Mva1269I	GAATGC	1 cut(s)	92
MvaI	CCWGG	2 cut(s)	101, 1113
MvnI	CGCG	1 cut(s)	568
MwoI	GCNNNNNNNGC	3 cut(s)	96, 256, 996
NdeI	CATATG	1 cut(s)	283
NdeII	GATC	5 cut(s)	103, 616, 853, 1195, 1267
NlaIII	CATG	7 cut(s)	175, 188, 636, 680, 748, 1035, 1277
NlaIV	GGNNCC	2 cut(s)	212, 1144
NmuCI	GTSAC	2 cut(s)	363, 1171
NsiI	ATGCAT	2 cut(s)	252, 750
NspI	RCATGY	3 cut(s)	636, 748, 1035
NspV	TTCGAA	1 cut(s)	73
PaeI	GCATGC	3 cut(s)	636, 748, 1035
PagI	TCATGA	1 cut(s)	184
PctI	GAATGC	1 cut(s)	92
PfeI	GAWTC	2 cut(s)	759, 1167
PflMI	CCANNNNNTGG	4 cut(s)	359, 713, 971, 1205
PinAI	ACCGGT	1 cut(s)	1058
PkrI	GCNGC	4 cut(s)	92, 547, 631, 1180
PleI	GAGTC	4 cut(s)	196, 388, 697, 1298
PpsI	GAGTC	4 cut(s)	196, 388, 697, 1298
Psp124BI	GAGCTC	1 cut(s)	57
Psp1406I	AACGTT	1 cut(s)	525
Psp6I	CCWGG	2 cut(s)	99, 1111
PspFI	CCCAGC	1 cut(s)	914
PspGI	CCWGG	2 cut(s)	99, 1111
PspN4I	GGNNCC	2 cut(s)	212, 1144
PspPI	GGNCC	1 cut(s)	1208
PsuI	RGATCY	3 cut(s)	103, 1195, 1267
PvuII	CAGCTG	2 cut(s)	710, 1253
RsaI	GTAC	4 cut(s)	441, 565, 983, 1107
RsaNI	GTAC	4 cut(s)	440, 564, 982, 1106
RseI	CAYNNNNRTG	1 cut(s)	861
SacI	GAGCTC	1 cut(s)	57
SalI	GTCGAC	1 cut(s)	880
SaqAI	TTAA	5 cut(s)	165, 219, 492, 1050, 1068
SatI	GCNGC	4 cut(s)	91, 546, 630, 1179
Sau3AI	GATC	5 cut(s)	103, 616, 853, 1195, 1267
Sau96I	GGNCC	1 cut(s)	1208
SchI	GAGTC	4 cut(s)	197, 389, 698, 1298
ScrFI	CCNGG	2 cut(s)	101, 1113
SduI	GDGCHC	2 cut(s)	57, 1147
SfaNI	GCATC	3 cut(s)	237, 757, 977
SfuI	TTCGAA	1 cut(s)	73
SmiMI	CAYNNNNRTG	1 cut(s)	861
SmlI	CTYRAG	1 cut(s)	1097
SmoI	CTYRAG	1 cut(s)	1097
SphI	GCATGC	3 cut(s)	636, 748, 1035
Sse9I	AATT	7 cut(s)	68, 341, 667, 717, 777, 845, 966
SsiI	CCGC	2 cut(s)	672, 1271
SspI	AATATT	2 cut(s)	388, 496
SspMI	CTAG	4 cut(s)	723, 765, 1163, 1212
SstI	GAGCTC	1 cut(s)	57
StyD4I	CCNGG	2 cut(s)	99, 1111
StyI	CCWWGG	1 cut(s)	1162
TaaI	ACNGT	1 cut(s)	563
TaiI	ACGT	2 cut(s)	528, 865
TaqI	TCGA	3 cut(s)	73, 881, 1063
TasI	AATT	7 cut(s)	68, 341, 667, 717, 777, 845, 966
TatI	WGTACW	1 cut(s)	439
TfiI	GAWTC	2 cut(s)	759, 1167
Tru1I	TTAA	5 cut(s)	165, 219, 492, 1050, 1068
Tru9I	TTAA	5 cut(s)	165, 219, 492, 1050, 1068
TscAI	CASTG	2 cut(s)	1180, 1297
TseFI	GTSAC	2 cut(s)	363, 1171
TseI	GCWGC	4 cut(s)	90, 545, 629, 1178
Tsp45I	GTSAC	2 cut(s)	363, 1171
TspDTI	ATGAA	3 cut(s)	173, 501, 917
TspGWI	ACGGA	1 cut(s)	948
TspRI	CASTG	2 cut(s)	1180, 1297
Van91I	CCANNNNNTGG	4 cut(s)	359, 713, 971, 1205
XagI	CCTNNNNNAGG	1 cut(s)	1151
XapI	RAATTY	3 cut(s)	68, 717, 777
XceI	RCATGY	3 cut(s)	636, 748, 1035
XmaJI	CCTAGG	1 cut(s)	1162
XmiI	GTMKAC	1 cut(s)	881
XspI	CTAG	4 cut(s)	723, 765, 1163, 1212
Zsp2I	ATGCAT	2 cut(s)	252, 750

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.