Rh4BG007100

Zinc finger, ZZ type

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0008857
Physical Location & Seq
Forward (+)
1090024 .. 1093484
3461 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG007100.1

Sequence Viewer

Length: 1383 bp
ATGAGTGCTCATTGCAAGTCTCGTGATATGGTGTTAAACTTAATATTCTGTTATCTTTTTTACCTTCTATCTTGTTTGGTAATAACATGTCTAAAGACTATTCATTTACTTTGCAGGGATCTTCTTTACAAGCCAGTTGTATTAGGATGTGGCCACATTTCATGTTTCTGGTGCGTCTTCAATTCCATGAGTGCTTGTCATGAATCCAATTGTCCAGTTTGTCGATTCCCATATAATCATTTCCCTAGTATCTGTAATTTGCTGCATTTCATACTCCAAAAGTTGTATCCGACAGTATATCAGAGAAGGGAAAAGCAAGTGGGAGAAGAAGAGAAGGAAGCTGGCTTCTTTTCACCTGAAGTTCAAAATCCGCTAGCTGGACCACAGCCTACTGAGTCAAATATTCCTGGAAATGTTGCACATTCTCCCCCTTGTCCAAAGTCAAAGAACTCTGAAAGTTGTTCTTCTGGGGTAGGAGAATCTCCTTCACTTGTAAATTCACCAGAAATGTTAGACCATGAAGCTAATAGTTCAAGCGTTTCAATATCTTCAAAGAATGTTGAACGAACTGAAAATGCAGCCATCCAAGAGCATTGTTTGCATGGTAATGATCTTGAAAATGGAACTCACAAGGAGATTTCTGTTTCTGATCTACTATGTGCTGCATGCAAGAACTTGCTCTATCAACCTGTTGTTCTCAATTGTGGCCATGTATATTGTAAATCTTGTATAAGCATCCCAGAAGATGGAATTTCTATGTGTCAGGTTTGTCAAAGCATGCATCCAAATGGATTTCCAAGTGTGTGCTTAGTTTTAGAGCATTTCTTGGAGGAGCAGTACCCTGAAGCTTATGCACAGAGACAAGCATCTTCACTAAAACAAGCTGACAGTCAGTTGAATGTTCATCAACGTGCGGGCCGATCATCATCAACACTTTCTGATGAGTACTTATCCTGCTGGTATGGTGTCGGGCCAAAAGTTCACTTTGGAATTGGTTGTGACTACTGTGGGATGTCTCCAATTATTGGAGAGAGGTACAGATGTAAAGATTGTGTGGAGCTAATGGGTTTTGACCTATGTGGAGCATGCTATAAAGCCCCTTTTAAGATGTATGGTCGATTCAATCAGCAACATAAACCAGAACACAAGCTTGAGATTGTACAGCCAGAGATGATAGATATCGTTCCCTACCAGTCAGATGAAGATGGCGCGGAGGTTATTGAACACCTAGGAAATATCCCTCCTGTTCCCATTCCACAAGAACCTGAAAATGGCTCTAACAATCTGCAAAATGGTTCTGCTGCTTTTGTGATGTCAGTTGATACTTCACGAGATCCAGATGATGATTTTAATGATTGTTTACTGGATTTGACTCAAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ZZ zf-C3HC4_3 zf-C3HC4_4 zf-RING_UBOX

Protein Analysis

460

Amino Acids

51.16

Weight (kDa)

5.2

Isoelectric Point (pI)

59.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ZZ PF00569 328 - 366 5.3e-07 Zinc finger, ZZ type
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 746, 1025
AccII CGCG 1 cut(s) 1211
AciI CCGC 3 cut(s) 371, 914, 1211
AclWI GGATC 2 cut(s) 126, 1328
AcoI YGGCCR 2 cut(s) 151, 706
AcsI RAATTY 2 cut(s) 496, 750
AcuI CTGAAG 2 cut(s) 378, 864
AfaI GTAC 4 cut(s) 839, 947, 1037, 1161
AfiI CCNNNNNNNGG 4 cut(s) 377, 746, 1025, 1271
AflIII ACRYGT 1 cut(s) 86
AjnI CCWGG 1 cut(s) 406
AluBI AGCT 7 cut(s) 341, 377, 524, 848, 884, 1060, 1150
AluI AGCT 7 cut(s) 341, 377, 524, 848, 884, 1060, 1150
Alw21I GWGCWC 1 cut(s) 10
Alw26I GTCTC 3 cut(s) 24, 853, 1020
AlwI GGATC 2 cut(s) 126, 1328
AoxI GGCC 4 cut(s) 151, 706, 916, 971
ApeKI GCWGC 4 cut(s) 262, 578, 662, 1301
ApoI RAATTY 2 cut(s) 496, 750
AspA2I CCTAGG 1 cut(s) 1228
AspLEI GCGC 1 cut(s) 1211
AspS9I GGNCC 3 cut(s) 380, 916, 971
AsuHPI GGTGA 2 cut(s) 345, 492
AsuNHI GCTAGC 1 cut(s) 373
AvaII GGWCC 1 cut(s) 380
AvrII CCTAGG 1 cut(s) 1228
BaeI ACNNNNGTAYC 2 cut(s) 1027, 1060
BalI TGGCCA 2 cut(s) 153, 708
BauI CACGAG 2 cut(s) 21, 1329
BbsI GAAGAC 1 cut(s) 169
Bbv12I GWGCWC 1 cut(s) 10
BbvI GCAGC 4 cut(s) 249, 590, 649, 1288
BccI CCATC 3 cut(s) 590, 740, 1199
BciT130I CCWGG 1 cut(s) 408
BciVI GTATCC 1 cut(s) 297
BcoDI GTCTC 3 cut(s) 24, 853, 1020
BfaI CTAG 3 cut(s) 246, 374, 1229
BfuI GTATCC 1 cut(s) 297
BisI GCNGC 4 cut(s) 263, 579, 663, 1302
BlnI CCTAGG 1 cut(s) 1228
BlsI GCNGC 4 cut(s) 264, 580, 664, 1303
BmcAI AGTACT 1 cut(s) 947
Bme1390I CCNGG 1 cut(s) 408
Bme18I GGWCC 1 cut(s) 380
BmgT120I GGNCC 3 cut(s) 380, 916, 971
BmrFI CCNGG 1 cut(s) 408
BmsI GCATC 3 cut(s) 744, 790, 875
BmtI GCTAGC 1 cut(s) 377
BpiI GAAGAC 1 cut(s) 169
BpuEI CTTGAG 2 cut(s) 1172, 1359
BsaBI GATNNNNATC 2 cut(s) 925, 1178
BsaJI CCNNGG 1 cut(s) 1228
BsaXI ACNNNNNCTCC 2 cut(s) 821, 851
Bsc4I CCNNNNNNNGG 4 cut(s) 377, 746, 1025, 1271
Bse1I ACTGG 4 cut(s) 134, 215, 1192, 1368
Bse3DI GCAATG 1 cut(s) 10
Bse8I GATNNNNATC 2 cut(s) 925, 1178
BseBI CCWGG 1 cut(s) 408
BseDI CCNNGG 1 cut(s) 1228
BseGI GGATG 5 cut(s) 152, 582, 735, 781, 1017
BseJI GATNNNNATC 2 cut(s) 925, 1178
BseLI CCNNNNNNNGG 4 cut(s) 377, 746, 1025, 1271
BseMI GCAATG 1 cut(s) 10
BseMII CTCAG 1 cut(s) 384
BseNI ACTGG 4 cut(s) 134, 215, 1192, 1368
BseRI GAGGAG 1 cut(s) 845
BseXI GCAGC 4 cut(s) 249, 590, 649, 1288
Bsh1236I CGCG 1 cut(s) 1211
BshFI GGCC 4 cut(s) 153, 708, 918, 973
BsiHKAI GWGCWC 1 cut(s) 10
BslI CCNNNNNNNGG 4 cut(s) 377, 746, 1025, 1271
BsmAI GTCTC 3 cut(s) 24, 853, 1020
BsnI GGCC 4 cut(s) 153, 708, 918, 973
Bsp1286I GDGCHC 1 cut(s) 10
Bsp1407I TGTACA 1 cut(s) 1159
Bsp143I GATC 5 cut(s) 118, 610, 649, 920, 1333
BspACI CCGC 3 cut(s) 371, 914, 1211
BspANI GGCC 4 cut(s) 153, 708, 918, 973
BspCNI CTCAG 1 cut(s) 385
BspFNI CGCG 1 cut(s) 1211
BspHI TCATGA 1 cut(s) 199
BspOI GCTAGC 1 cut(s) 377
BspPI GGATC 2 cut(s) 126, 1328
BsrDI GCAATG 1 cut(s) 10
BsrGI TGTACA 1 cut(s) 1159
BsrI ACTGG 4 cut(s) 134, 215, 1192, 1368
BssECI CCNNGG 1 cut(s) 1228
BssMI GATC 5 cut(s) 118, 610, 649, 920, 1333
BssSI CACGAG 2 cut(s) 21, 1329
BssT1I CCWWGG 1 cut(s) 1228
Bst2BI CACGAG 2 cut(s) 21, 1329
Bst2UI CCWGG 1 cut(s) 408
Bst4CI ACNGT 3 cut(s) 295, 890, 1007
Bst6I CTCTTC 1 cut(s) 324
BstAPI GCANNNNNTGC 1 cut(s) 598
BstAUI TGTACA 1 cut(s) 1159
BstC8I GCNNGC 6 cut(s) 343, 375, 667, 779, 916, 1087
BstDEI CTNAG 2 cut(s) 393, 808
BstF5I GGATG 5 cut(s) 152, 582, 735, 781, 1017
BstFNI CGCG 1 cut(s) 1211
BstHHI GCGC 1 cut(s) 1211
BstKTI GATC 5 cut(s) 121, 613, 652, 923, 1336
BstMAI GTCTC 3 cut(s) 24, 853, 1020
BstMBI GATC 5 cut(s) 118, 610, 649, 920, 1333
BstMWI GCNNNNNNNGC 1 cut(s) 598
BstNI CCWGG 1 cut(s) 408
BstNSI RCATGY 4 cut(s) 90, 669, 781, 1089
BstSCI CCNGG 1 cut(s) 406
BstUI CGCG 1 cut(s) 1211
BstV1I GCAGC 4 cut(s) 249, 590, 649, 1288
BstV2I GAAGAC 1 cut(s) 169
BstX2I RGATCY 2 cut(s) 118, 1333
BstYI RGATCY 2 cut(s) 118, 1333
BsuI GTATCC 1 cut(s) 297
BsuRI GGCC 4 cut(s) 153, 708, 918, 973
BtsCI GGATG 5 cut(s) 152, 582, 735, 781, 1017
Cac8I GCNNGC 6 cut(s) 343, 375, 667, 779, 916, 1087
CciI TCATGA 1 cut(s) 199
CfoI GCGC 1 cut(s) 1211
Cfr13I GGNCC 3 cut(s) 380, 916, 971
CseI GACGC 1 cut(s) 163
Csp6I GTAC 4 cut(s) 838, 946, 1036, 1160
CviQI GTAC 4 cut(s) 838, 946, 1036, 1160
DdeI CTNAG 2 cut(s) 393, 808
DpnI GATC 5 cut(s) 120, 612, 651, 922, 1335
DpnII GATC 5 cut(s) 118, 610, 649, 920, 1333
EaeI YGGCCR 2 cut(s) 151, 706
Eam1104I CTCTTC 1 cut(s) 324
EarI CTCTTC 1 cut(s) 324
Eco130I CCWWGG 1 cut(s) 1228
Eco32I GATATC 1 cut(s) 1180
Eco47I GGWCC 1 cut(s) 380
Eco57I CTGAAG 2 cut(s) 378, 864
EcoRII CCWGG 1 cut(s) 406
EcoRV GATATC 1 cut(s) 1180
EcoT14I CCWWGG 1 cut(s) 1228
EcoT22I ATGCAT 1 cut(s) 783
ErhI CCWWGG 1 cut(s) 1228
FalI AAGNNNNNCTT 2 cut(s) 448, 480
FauI CCCGC 1 cut(s) 907
Fnu4HI GCNGC 4 cut(s) 263, 579, 663, 1302
FokI GGATG 5 cut(s) 159, 569, 722, 768, 1024
Fsp4HI GCNGC 4 cut(s) 263, 579, 663, 1302
FspBI CTAG 3 cut(s) 246, 374, 1229
GlaI GCGC 1 cut(s) 1210
GluI GCNGC 4 cut(s) 263, 579, 663, 1302
HaeIII GGCC 4 cut(s) 153, 708, 918, 973
HgaI GACGC 1 cut(s) 163
HhaI GCGC 1 cut(s) 1211
Hin6I GCGC 1 cut(s) 1209
HinP1I GCGC 1 cut(s) 1209
HindIII AAGCTT 2 cut(s) 846, 1148
HinfI GANTC 6 cut(s) 203, 225, 395, 479, 1119, 1372
HphI GGTGA 2 cut(s) 345, 492
Hpy166II GTNNAC 2 cut(s) 982, 1361
Hpy188I TCNGA 6 cut(s) 291, 303, 454, 649, 940, 1198
Hpy188III TCNNGA 6 cut(s) 23, 200, 614, 1329, 1337, 1376
Hpy8I GTNNAC 2 cut(s) 982, 1361
HpyAV CCTTC 4 cut(s) 74, 300, 328, 495
HpyCH4III ACNGT 3 cut(s) 295, 890, 1007
HpyCH4IV ACGT 1 cut(s) 910
HpyF10VI GCNNNNNNNGC 1 cut(s) 598
HpyF3I CTNAG 2 cut(s) 393, 808
HpySE526I ACGT 1 cut(s) 910
HspAI GCGC 1 cut(s) 1209
Kzo9I GATC 5 cut(s) 118, 610, 649, 920, 1333
LmnI GCTCC 3 cut(s) 832, 1057, 1082
Lsp1109I GCAGC 4 cut(s) 249, 590, 649, 1288
LweI GCATC 3 cut(s) 744, 790, 875
MaeI CTAG 3 cut(s) 246, 374, 1229
MaeII ACGT 1 cut(s) 910
MaeIII GTNAC 1 cut(s) 998
MalI GATC 5 cut(s) 120, 612, 651, 922, 1335
MboI GATC 5 cut(s) 118, 610, 649, 920, 1333
MboII GAAGA 9 cut(s) 113, 169, 338, 341, 456, 540, 755, 861, 1214
MfeI CAATTG 2 cut(s) 208, 700
MflI RGATCY 2 cut(s) 118, 1333
MhlI GDGCHC 1 cut(s) 10
MlsI TGGCCA 2 cut(s) 153, 708
MluCI AATT 8 cut(s) 181, 208, 256, 496, 700, 750, 990, 1020
MluNI TGGCCA 2 cut(s) 153, 708
MlyI GAGTC 2 cut(s) 404, 1366
MmeI TCCRAC 1 cut(s) 314
MnlI CCTC 4 cut(s) 823, 1026, 1207, 1251
Mox20I TGGCCA 2 cut(s) 153, 708
Mph1103I ATGCAT 1 cut(s) 783
MscI TGGCCA 2 cut(s) 153, 708
MseI TTAA 4 cut(s) 35, 41, 1104, 1350
MslI CAYNNNNRTG 3 cut(s) 606, 786, 909
Msp20I TGGCCA 2 cut(s) 153, 708
MspR9I CCNGG 1 cut(s) 408
MunI CAATTG 2 cut(s) 208, 700
MvaI CCWGG 1 cut(s) 408
MvnI CGCG 1 cut(s) 1211
MwoI GCNNNNNNNGC 1 cut(s) 598
NdeII GATC 5 cut(s) 118, 610, 649, 920, 1333
NheI GCTAGC 1 cut(s) 373
NmuCI GTSAC 1 cut(s) 998
NsiI ATGCAT 1 cut(s) 783
NspI RCATGY 4 cut(s) 90, 669, 781, 1089
PaeI GCATGC 3 cut(s) 669, 781, 1089
PagI TCATGA 1 cut(s) 199
PciI ACATGT 1 cut(s) 86
PcsI WCGNNNNNNNCGW 1 cut(s) 916
PfeI GAWTC 4 cut(s) 203, 225, 479, 1119
PflMI CCANNNNNTGG 2 cut(s) 746, 1025
PfoI TCCNGGA 1 cut(s) 406
PkrI GCNGC 4 cut(s) 264, 580, 664, 1303
PleI GAGTC 2 cut(s) 403, 1366
PpsI GAGTC 2 cut(s) 403, 1366
PscI ACATGT 1 cut(s) 86
Psp6I CCWGG 1 cut(s) 406
PspGI CCWGG 1 cut(s) 406
PspPI GGNCC 3 cut(s) 380, 916, 971
PsuI RGATCY 2 cut(s) 118, 1333
RsaI GTAC 4 cut(s) 839, 947, 1037, 1161
RsaNI GTAC 4 cut(s) 838, 946, 1036, 1160
RseI CAYNNNNRTG 3 cut(s) 606, 786, 909
SaqAI TTAA 4 cut(s) 35, 41, 1104, 1350
SatI GCNGC 4 cut(s) 263, 579, 663, 1302
Sau3AI GATC 5 cut(s) 118, 610, 649, 920, 1333
Sau96I GGNCC 3 cut(s) 380, 916, 971
ScaI AGTACT 1 cut(s) 947
SchI GAGTC 2 cut(s) 404, 1366
ScrFI CCNGG 1 cut(s) 408
SduI GDGCHC 1 cut(s) 10
SfaNI GCATC 3 cut(s) 744, 790, 875
SinI GGWCC 1 cut(s) 380
SmiMI CAYNNNNRTG 3 cut(s) 606, 786, 909
SmlI CTYRAG 2 cut(s) 1151, 1374
SmoI CTYRAG 2 cut(s) 1151, 1374
SphI GCATGC 3 cut(s) 669, 781, 1089
Sse9I AATT 8 cut(s) 181, 208, 256, 496, 700, 750, 990, 1020
SsiI CCGC 3 cut(s) 371, 914, 1211
SspI AATATT 2 cut(s) 45, 403
SspMI CTAG 3 cut(s) 246, 374, 1229
StyD4I CCNGG 1 cut(s) 406
StyI CCWWGG 1 cut(s) 1228
TaaI ACNGT 3 cut(s) 295, 890, 1007
TaiI ACGT 1 cut(s) 913
TaqI TCGA 2 cut(s) 223, 1117
TasI AATT 8 cut(s) 181, 208, 256, 496, 700, 750, 990, 1020
TatI WGTACW 2 cut(s) 945, 1159
TfiI GAWTC 4 cut(s) 203, 225, 479, 1119
Tru1I TTAA 4 cut(s) 35, 41, 1104, 1350
Tru9I TTAA 4 cut(s) 35, 41, 1104, 1350
TseFI GTSAC 1 cut(s) 998
TseI GCWGC 4 cut(s) 262, 578, 662, 1301
Tsp45I GTSAC 1 cut(s) 998
TspDTI ATGAA 7 cut(s) 92, 150, 216, 259, 534, 893, 1215
Van91I CCANNNNNTGG 2 cut(s) 746, 1025
VpaK11BI GGWCC 1 cut(s) 380
XapI RAATTY 2 cut(s) 496, 750
XceI RCATGY 4 cut(s) 90, 669, 781, 1089
XmaJI CCTAGG 1 cut(s) 1228
XspI CTAG 3 cut(s) 246, 374, 1229
ZrmI AGTACT 1 cut(s) 947
Zsp2I ATGCAT 1 cut(s) 783
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.