Rh4AG010100

Zinc finger, ZZ type

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0008857
Physical Location & Seq
Forward (+)
1661913 .. 1665701
3789 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG010100.1

Sequence Viewer

Length: 1251 bp
ATGGAAAACAAGGAAACCTTAATAAAACAAGAACAAGACCTTGAACCTGAGGATTTCCCTGAAGAGTTCCAGTGCTGTGTTTGCCTGGATCTTCTTTACAAGCCAGTTGTATTAGGATGTGGCCACATTTCATGTTTCTGGTGCGTCTTCAATTCCATGAGTGCTTGTCATGAATCCAATTGTCCAGTTTGTCGATTCCCATATAATCATTTCCCTAGTATCTGTAATTTGCTGCATTTCATACTCCAAAAGTTGTATCCGACAGCATATCAGAGAAGGGAAAAGCAAGTGGGAGAAGAAGAGAAGGAAGCTGGCTTCTTTTCACCTGAAGTTCAAAATCCGCTAGCCGGACCACAGCCTACTGAGTCAAATATTCCTGGAAATGTTGCACATTCTCCCCCTTGTCCAAAGTCAAAGAACTCTGAAAGTTGTTCTTCTGGGGTAGGAGAATCTCCTTCACTTGTAAATTCACCAGAAATGTTAGACCATGAAGCTAATAGTTCAAGCGTTTCAATATCTTCAAAGAATGTTGAACGAACTGAAAATGCAGCCATCCAAGAGCATTGTTTGCATGGTAATGATCTTGAAAATGGAACTCACAAGGAGATTTCTGTTTCTGATCTACTATGTGCTGCATGCAAGAACTTGCTCTATCAACCTGTTGTTCTCAATTGTGGCCATGTATATTGTAAATCTTGTATAAGCATCCCAGAAGATGGAATTTCTAGGTGTCAGGTTTGTCAAAGCATGCATCCAAATGGATTTCCAAGTGTGTGCTTAGTTTTAGAGCATTTCTTGGAGGAGCAGTACCCTGAAGCTTATGCACAGAGACAAGCATCTTCACTAAAACAAGCTGACAGTCAGTTGAATGTTCGTCAACGTGCGGGCCGATCATCATCAACACTTTCTGATGAATACTTATCCTGCTGGTATGGTGTCGGGCCAAAAGTTCACTTTGGAATTGGTTGTGACTACTGTGGGATGTCTCCAATTATTGGAGAGAGGTACAGATGTAAAGATTGTGTGGAGCTAATGGGTTTTGACCTATGTGGAGCATGCTATAAAGCCCCTTTTAAGATGTCTGGTCGATTCAATCAGCAACATAAACCAGAACACAAGCTTGAGATTGTACAGCCACAGATGATAGATATCGTTCCCTACCAGTCAGATGAAGATGGCGCCGAGGTTATGGAACACCTAGGAAATGTCCCTCCTGTTCCCATTCCACAAGAACCAGAAAATGGCTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ZZ zf-C3HC4_3 zf-C3HC4_4 zf-RING_UBOX

Protein Analysis

416

Amino Acids

46.27

Weight (kDa)

5.08

Isoelectric Point (pI)

65.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_UBOX PF13445 210 - 244 8.4e-06 RING-type zinc-finger
ZZ PF00569 318 - 356 5.5e-07 Zinc finger, ZZ type
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1178
AccB7I CCANNNNNTGG 3 cut(s) 716, 995, 1241
AciI CCGC 2 cut(s) 341, 884
AclWI GGATC 1 cut(s) 96
AcoI YGGCCR 2 cut(s) 121, 676
AcsI RAATTY 2 cut(s) 466, 720
AcuI CTGAAG 3 cut(s) 81, 348, 834
AcyI GRCGYC 1 cut(s) 1179
AfaI GTAC 3 cut(s) 809, 1007, 1131
AfiI CCNNNNNNNGG 4 cut(s) 347, 716, 995, 1241
AjnI CCWGG 2 cut(s) 84, 376
AluBI AGCT 6 cut(s) 311, 494, 818, 854, 1030, 1120
AluI AGCT 6 cut(s) 311, 494, 818, 854, 1030, 1120
Alw26I GTCTC 2 cut(s) 823, 990
AlwI GGATC 1 cut(s) 96
AoxI GGCC 4 cut(s) 121, 676, 886, 941
ApeKI GCWGC 3 cut(s) 232, 548, 632
ApoI RAATTY 2 cut(s) 466, 720
AspA2I CCTAGG 1 cut(s) 1198
AspLEI GCGC 1 cut(s) 1181
AspS9I GGNCC 3 cut(s) 350, 886, 941
AsuHPI GGTGA 2 cut(s) 315, 462
AsuNHI GCTAGC 1 cut(s) 343
AvaII GGWCC 1 cut(s) 350
AvrII CCTAGG 1 cut(s) 1198
AxyI CCTNAGG 1 cut(s) 48
BaeI ACNNNNGTAYC 2 cut(s) 997, 1030
BalI TGGCCA 2 cut(s) 123, 678
BanI GGYRCC 1 cut(s) 1178
BbsI GAAGAC 1 cut(s) 139
BbvI GCAGC 3 cut(s) 219, 560, 619
BccI CCATC 3 cut(s) 560, 710, 1169
BciT130I CCWGG 2 cut(s) 86, 378
BciVI GTATCC 1 cut(s) 267
BcoDI GTCTC 2 cut(s) 823, 990
BfaI CTAG 4 cut(s) 216, 344, 726, 1199
BfoI RGCGCY 1 cut(s) 1182
BfuI GTATCC 1 cut(s) 267
BisI GCNGC 3 cut(s) 233, 549, 633
BlnI CCTAGG 1 cut(s) 1198
BlsI GCNGC 3 cut(s) 234, 550, 634
Bme1390I CCNGG 2 cut(s) 86, 378
Bme18I GGWCC 1 cut(s) 350
BmgT120I GGNCC 3 cut(s) 350, 886, 941
BmiI GGNNCC 1 cut(s) 1180
BmrFI CCNGG 2 cut(s) 86, 378
BmsI GCATC 3 cut(s) 714, 760, 845
BmtI GCTAGC 1 cut(s) 347
BpiI GAAGAC 1 cut(s) 139
BpuEI CTTGAG 1 cut(s) 1142
BsaBI GATNNNNATC 2 cut(s) 895, 1148
BsaHI GRCGYC 1 cut(s) 1179
BsaJI CCNNGG 2 cut(s) 1182, 1198
BsaXI ACNNNNNCTCC 2 cut(s) 791, 821
Bsc4I CCNNNNNNNGG 4 cut(s) 347, 716, 995, 1241
Bse1I ACTGG 4 cut(s) 70, 104, 185, 1162
Bse21I CCTNAGG 1 cut(s) 48
Bse8I GATNNNNATC 2 cut(s) 895, 1148
BseBI CCWGG 2 cut(s) 86, 378
BseDI CCNNGG 2 cut(s) 1182, 1198
BseGI GGATG 5 cut(s) 122, 552, 705, 751, 987
BseJI GATNNNNATC 2 cut(s) 895, 1148
BseLI CCNNNNNNNGG 4 cut(s) 347, 716, 995, 1241
BseMII CTCAG 2 cut(s) 39, 354
BseNI ACTGG 4 cut(s) 70, 104, 185, 1162
BseRI GAGGAG 1 cut(s) 815
BseXI GCAGC 3 cut(s) 219, 560, 619
BshFI GGCC 4 cut(s) 123, 678, 888, 943
BshNI GGYRCC 1 cut(s) 1178
BsiSI CCGG 1 cut(s) 348
BslFI GGGAC 1 cut(s) 1193
BslI CCNNNNNNNGG 4 cut(s) 347, 716, 995, 1241
BsmAI GTCTC 2 cut(s) 823, 990
BsmFI GGGAC 1 cut(s) 1193
BsnI GGCC 4 cut(s) 123, 678, 888, 943
Bsp1407I TGTACA 1 cut(s) 1129
Bsp143I GATC 4 cut(s) 88, 580, 619, 890
BspACI CCGC 2 cut(s) 341, 884
BspANI GGCC 4 cut(s) 123, 678, 888, 943
BspCNI CTCAG 2 cut(s) 40, 355
BspHI TCATGA 1 cut(s) 169
BspLI GGNNCC 1 cut(s) 1180
BspOI GCTAGC 1 cut(s) 347
BspPI GGATC 1 cut(s) 96
BspT107I GGYRCC 1 cut(s) 1178
BsrGI TGTACA 1 cut(s) 1129
BsrI ACTGG 4 cut(s) 70, 104, 185, 1162
BssECI CCNNGG 2 cut(s) 1182, 1198
BssMI GATC 4 cut(s) 88, 580, 619, 890
BssNI GRCGYC 1 cut(s) 1179
BssT1I CCWWGG 1 cut(s) 1198
Bst2UI CCWGG 2 cut(s) 86, 378
Bst4CI ACNGT 2 cut(s) 860, 977
Bst6I CTCTTC 2 cut(s) 57, 294
BstACI GRCGYC 1 cut(s) 1179
BstAPI GCANNNNNTGC 1 cut(s) 568
BstAUI TGTACA 1 cut(s) 1129
BstC8I GCNNGC 6 cut(s) 313, 345, 637, 749, 886, 1057
BstDEI CTNAG 3 cut(s) 48, 363, 778
BstF5I GGATG 5 cut(s) 122, 552, 705, 751, 987
BstH2I RGCGCY 1 cut(s) 1182
BstHHI GCGC 1 cut(s) 1181
BstKTI GATC 4 cut(s) 91, 583, 622, 893
BstMAI GTCTC 2 cut(s) 823, 990
BstMBI GATC 4 cut(s) 88, 580, 619, 890
BstMWI GCNNNNNNNGC 2 cut(s) 81, 568
BstNI CCWGG 2 cut(s) 86, 378
BstNSI RCATGY 3 cut(s) 639, 751, 1059
BstSCI CCNGG 2 cut(s) 84, 376
BstV1I GCAGC 3 cut(s) 219, 560, 619
BstV2I GAAGAC 1 cut(s) 139
BstX2I RGATCY 1 cut(s) 88
BstYI RGATCY 1 cut(s) 88
Bsu36I CCTNAGG 1 cut(s) 48
BsuI GTATCC 1 cut(s) 267
BsuRI GGCC 4 cut(s) 123, 678, 888, 943
BtsCI GGATG 5 cut(s) 122, 552, 705, 751, 987
BtsIMutI CAGTG 1 cut(s) 77
Cac8I GCNNGC 6 cut(s) 313, 345, 637, 749, 886, 1057
CciI TCATGA 1 cut(s) 169
CfoI GCGC 1 cut(s) 1181
Cfr13I GGNCC 3 cut(s) 350, 886, 941
CseI GACGC 1 cut(s) 133
Csp6I GTAC 3 cut(s) 808, 1006, 1130
CviAII CATG 9 cut(s) 132, 157, 170, 488, 572, 636, 680, 748, 1056
CviQI GTAC 3 cut(s) 808, 1006, 1130
DdeI CTNAG 3 cut(s) 48, 363, 778
DinI GGCGCC 1 cut(s) 1180
DpnI GATC 4 cut(s) 90, 582, 621, 892
DpnII GATC 4 cut(s) 88, 580, 619, 890
EaeI YGGCCR 2 cut(s) 121, 676
Eam1104I CTCTTC 2 cut(s) 57, 294
EarI CTCTTC 2 cut(s) 57, 294
Eco130I CCWWGG 1 cut(s) 1198
Eco32I GATATC 1 cut(s) 1150
Eco47I GGWCC 1 cut(s) 350
Eco57I CTGAAG 3 cut(s) 81, 348, 834
Eco81I CCTNAGG 1 cut(s) 48
EcoRII CCWGG 2 cut(s) 84, 376
EcoRV GATATC 1 cut(s) 1150
EcoT14I CCWWGG 1 cut(s) 1198
EcoT22I ATGCAT 1 cut(s) 753
EgeI GGCGCC 1 cut(s) 1180
EheI GGCGCC 1 cut(s) 1180
ErhI CCWWGG 1 cut(s) 1198
FaeI CATG 9 cut(s) 135, 160, 173, 491, 575, 639, 683, 751, 1059
FalI AAGNNNNNCTT 3 cut(s) 34, 418, 450
FaqI GGGAC 1 cut(s) 1193
FatI CATG 9 cut(s) 131, 156, 169, 487, 571, 635, 679, 747, 1055
FauI CCCGC 1 cut(s) 877
Fnu4HI GCNGC 3 cut(s) 233, 549, 633
FokI GGATG 5 cut(s) 129, 539, 692, 738, 994
Fsp4HI GCNGC 3 cut(s) 233, 549, 633
FspBI CTAG 4 cut(s) 216, 344, 726, 1199
GlaI GCGC 1 cut(s) 1180
GluI GCNGC 3 cut(s) 233, 549, 633
HaeII RGCGCY 1 cut(s) 1182
HaeIII GGCC 4 cut(s) 123, 678, 888, 943
HapII CCGG 1 cut(s) 348
HgaI GACGC 1 cut(s) 133
HhaI GCGC 1 cut(s) 1181
Hin1I GRCGYC 1 cut(s) 1179
Hin1II CATG 9 cut(s) 135, 160, 173, 491, 575, 639, 683, 751, 1059
Hin6I GCGC 1 cut(s) 1179
HinP1I GCGC 1 cut(s) 1179
HincII GTYRAC 1 cut(s) 878
HindII GTYRAC 1 cut(s) 878
HindIII AAGCTT 2 cut(s) 816, 1118
HinfI GANTC 5 cut(s) 173, 195, 365, 449, 1089
HpaII CCGG 1 cut(s) 348
HphI GGTGA 2 cut(s) 315, 462
Hpy166II GTNNAC 2 cut(s) 878, 952
Hpy188I TCNGA 6 cut(s) 261, 273, 424, 619, 910, 1168
Hpy188III TCNNGA 2 cut(s) 170, 584
Hpy8I GTNNAC 2 cut(s) 878, 952
HpyAV CCTTC 3 cut(s) 270, 298, 465
HpyCH4III ACNGT 2 cut(s) 860, 977
HpyCH4IV ACGT 1 cut(s) 880
HpyCH4V TGCA 8 cut(s) 235, 389, 548, 571, 635, 639, 751, 824
HpyF10VI GCNNNNNNNGC 2 cut(s) 81, 568
HpyF3I CTNAG 3 cut(s) 48, 363, 778
HpySE526I ACGT 1 cut(s) 880
Hsp92I GRCGYC 1 cut(s) 1179
Hsp92II CATG 9 cut(s) 135, 160, 173, 491, 575, 639, 683, 751, 1059
HspAI GCGC 1 cut(s) 1179
KasI GGCGCC 1 cut(s) 1178
Kzo9I GATC 4 cut(s) 88, 580, 619, 890
LmnI GCTCC 3 cut(s) 802, 1027, 1052
Lsp1109I GCAGC 3 cut(s) 219, 560, 619
LweI GCATC 3 cut(s) 714, 760, 845
MaeI CTAG 4 cut(s) 216, 344, 726, 1199
MaeII ACGT 1 cut(s) 880
MaeIII GTNAC 1 cut(s) 968
MalI GATC 4 cut(s) 90, 582, 621, 892
MboI GATC 4 cut(s) 88, 580, 619, 890
MfeI CAATTG 2 cut(s) 178, 670
MflI RGATCY 1 cut(s) 88
MlsI TGGCCA 2 cut(s) 123, 678
MluCI AATT 8 cut(s) 151, 178, 226, 466, 670, 720, 960, 990
MluNI TGGCCA 2 cut(s) 123, 678
Mly113I GGCGCC 1 cut(s) 1179
MlyI GAGTC 1 cut(s) 374
MmeI TCCRAC 1 cut(s) 284
MnlI CCTC 5 cut(s) 43, 793, 996, 1177, 1221
Mox20I TGGCCA 2 cut(s) 123, 678
Mph1103I ATGCAT 1 cut(s) 753
MscI TGGCCA 2 cut(s) 123, 678
MseI TTAA 3 cut(s) 20, 1074, 1249
MslI CAYNNNNRTG 2 cut(s) 576, 756
Msp20I TGGCCA 2 cut(s) 123, 678
MspI CCGG 1 cut(s) 348
MspR9I CCNGG 2 cut(s) 86, 378
MunI CAATTG 2 cut(s) 178, 670
MvaI CCWGG 2 cut(s) 86, 378
MwoI GCNNNNNNNGC 2 cut(s) 81, 568
NarI GGCGCC 1 cut(s) 1179
NdeII GATC 4 cut(s) 88, 580, 619, 890
NheI GCTAGC 1 cut(s) 343
NlaIII CATG 9 cut(s) 135, 160, 173, 491, 575, 639, 683, 751, 1059
NlaIV GGNNCC 1 cut(s) 1180
NmeAIII GCCGAG 1 cut(s) 1207
NmuCI GTSAC 1 cut(s) 968
NsiI ATGCAT 1 cut(s) 753
NspI RCATGY 3 cut(s) 639, 751, 1059
PaeI GCATGC 3 cut(s) 639, 751, 1059
PagI TCATGA 1 cut(s) 169
PcsI WCGNNNNNNNCGW 1 cut(s) 886
PfeI GAWTC 4 cut(s) 173, 195, 449, 1089
PflMI CCANNNNNTGG 3 cut(s) 716, 995, 1241
PfoI TCCNGGA 1 cut(s) 376
PkrI GCNGC 3 cut(s) 234, 550, 634
PleI GAGTC 1 cut(s) 373
PluTI GGCGCC 1 cut(s) 1182
PpsI GAGTC 1 cut(s) 373
Psp6I CCWGG 2 cut(s) 84, 376
PspGI CCWGG 2 cut(s) 84, 376
PspN4I GGNNCC 1 cut(s) 1180
PspPI GGNCC 3 cut(s) 350, 886, 941
PsuI RGATCY 1 cut(s) 88
RsaI GTAC 3 cut(s) 809, 1007, 1131
RsaNI GTAC 3 cut(s) 808, 1006, 1130
RseI CAYNNNNRTG 2 cut(s) 576, 756
SaqAI TTAA 3 cut(s) 20, 1074, 1249
SatI GCNGC 3 cut(s) 233, 549, 633
Sau3AI GATC 4 cut(s) 88, 580, 619, 890
Sau96I GGNCC 3 cut(s) 350, 886, 941
SchI GAGTC 1 cut(s) 374
ScrFI CCNGG 2 cut(s) 86, 378
SfaNI GCATC 3 cut(s) 714, 760, 845
SfoI GGCGCC 1 cut(s) 1180
SinI GGWCC 1 cut(s) 350
SmiMI CAYNNNNRTG 2 cut(s) 576, 756
SmlI CTYRAG 1 cut(s) 1121
SmoI CTYRAG 1 cut(s) 1121
SphI GCATGC 3 cut(s) 639, 751, 1059
Sse9I AATT 8 cut(s) 151, 178, 226, 466, 670, 720, 960, 990
SsiI CCGC 2 cut(s) 341, 884
SspDI GGCGCC 1 cut(s) 1178
SspI AATATT 1 cut(s) 373
SspMI CTAG 4 cut(s) 216, 344, 726, 1199
StyD4I CCNGG 2 cut(s) 84, 376
StyI CCWWGG 1 cut(s) 1198
TaaI ACNGT 2 cut(s) 860, 977
TaiI ACGT 1 cut(s) 883
TaqI TCGA 2 cut(s) 193, 1087
TasI AATT 8 cut(s) 151, 178, 226, 466, 670, 720, 960, 990
TatI WGTACW 1 cut(s) 1129
TfiI GAWTC 4 cut(s) 173, 195, 449, 1089
Tru1I TTAA 3 cut(s) 20, 1074, 1249
Tru9I TTAA 3 cut(s) 20, 1074, 1249
TscAI CASTG 1 cut(s) 77
TseFI GTSAC 1 cut(s) 968
TseI GCWGC 3 cut(s) 232, 548, 632
Tsp45I GTSAC 1 cut(s) 968
TspDTI ATGAA 6 cut(s) 120, 186, 229, 504, 927, 1185
TspRI CASTG 1 cut(s) 77
Van91I CCANNNNNTGG 3 cut(s) 716, 995, 1241
VpaK11BI GGWCC 1 cut(s) 350
XapI RAATTY 2 cut(s) 466, 720
XceI RCATGY 3 cut(s) 639, 751, 1059
XmaJI CCTAGG 1 cut(s) 1198
XspI CTAG 4 cut(s) 216, 344, 726, 1199
Zsp2I ATGCAT 1 cut(s) 753
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.