Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0008938

Physical Location & Seq

Genomic Coordinates Reverse (-)

1805966 .. 1809223

Gene Structure 3258 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1016700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1308 bp

ATGGTCTTAGCCAGTCAAGAAAACTTGGTCTATGGCATCAAGCTTTCATCGGTCGGGCCAGCCAGGGTTACCGGTTCGGACGTGGTTCATAACCCGAGTGGCATGGACTTGGCTATGAAGCTTCACTACCTTAGAGGAGTTTACTTCTTTGATAGCCAGGCGGCTCAAGGCCTGACCACTAACCGCATAAAGGAAGCTATGTTCACATGGTTATGTGTCAGCTACCAGACATGCGGCCGGTTTCGGAGATCCGAATCCGGCCGGCCCTATCTGAAGTGTAACGATTGCGGTGCGAGGTATCTTGAAGCGCAGTGTGATAAAACCATCGATGAATGGCTAGGGATGGACTGCTCTCTTCAGAAGCTGCTTGCTTCTAACCAAATCATTGGCCCTGAACTATTCTTCTCTCCAGTTGTTCTCTTCCAGGTTACTTATTTCAAATGTGGGGGAATGTCACTAGGCCTCAGCTGGTCTCATGTCCTCGGAGATGCATTTGCAGCATCAGATTTCATCAACGGCTTAGGGCAAGTCATGTCTGGTCTGGAGCCCAGCAGGCTACCAAATTATGCAAAACCGAACACAAATGTTCAACAAAAACTTGCAAAAAATCCATCACCCCCACCACTCTCCATCAGACACGTGGACTCGGTTGGTGACTATTGGATAAGTCCCAATAAATGCAAGATGGAAACATTCTCGTTCACTGTAACTGCCACGCAGCTAAACAACTTGCAAGTCAAGATATTAGGTCCAATTCAAAGTGACCAAATTCCAATTTTTGAACTTATTTGTGCCCTAATTTGGAAATGCGTAGCCACAGTTAGAGAAGGGCCACAACCAAAATTGGTGACAATTTGCAAAAATGACACAAATAATAGAACAGAAGGTAACAGTCAGACTATAAGCACAGTCGAGGCAGATTTTAGGGTTTCGGATATGGATCTGAAAGAACTAGCAAACTTGCTAGCCAAGCAAGCCGGCCAGAACGAGAAAACACGAATCGAAGAGGCCATAGAGAAAGAGAATGGAGTAGCTGATTTTGTTGTGTATGGAGCCAACTTGACGTTTGTGAACTGGGAAGATGTCGATTTTTATGGGTTGGAAGTGAAGGGACATAAGCCGGTTTGCGTGCATTATAACATACAAGGTGTTGGTGATGAAGGAGCTGTTTTGTTGCTGCCTGCTGGGGCTAAAGAGTTGGGTGATGGAGGAAGAGTGGTGACTGTTATTCTGCCAGAGAAAGAAGTGTTTGGGGTCAAATCTGAGTTGAGGAAAAATGATCTGCTGTTTGGTAATGAGCTTGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000038 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006629 GO:0006631 GO:0006633 GO:0007275 GO:0008150 GO:0008152 GO:0008610 GO:0009058 GO:0009507 GO:0009534 GO:0009535 GO:0009536 GO:0009555 GO:0009579 GO:0009987 GO:0010025 GO:0010166 GO:0016020 GO:0016053 GO:0019752 GO:0031976 GO:0031984 GO:0032501 GO:0032502 GO:0032787 GO:0034357 GO:0042651 GO:0042761 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0048229 GO:0048856 GO:0055035 GO:0071704 GO:0072330 GO:1901568 GO:1901570 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Transferase

Protein Analysis

436

Amino Acids

47.99

Weight (kDa)

5.57

Isoelectric Point (pI)

33.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Transferase	PF02458	33 - 422	4.6e-34	Transferase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008938)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G23840 AT4G13840
fragaria_vesca	FvH4_4g13360
malus_domestica	MD04G1016700.v1.1 MD04G1016900.v1.1
prunus_persica	Prupe.1G163500_v2.0.a1
pyrus_communis	pycom04g01280 pycom04g01300
rosa_chinensis	RchiOBHm_Chr4g0413701
rosa_laevigata	RLG00000008200
rosa_multiflora	Rmu_co8063286.1_g000001 Rmu_co8298563.1_g000001 Rmu_sc0004133.1_g000007 Rmu_sc0033890.1_g000002
rosa_roxburghii	Rroxscaffold_5G00357640
rosa_rugosa	Rorug04G0122700
rosa_samantha	Rh4AG181900 Rh4BG181000 Rh4BG181100 Rh4CG193100 Rh4DG177500
rosa_wichuraiana	Rw4G015220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1137
AciI	CCGC	4 cut(s)	161, 184, 234, 288
AclWI	GGATC	2 cut(s)	243, 948
AcoI	YGGCCR	3 cut(s)	235, 259, 979
AcsI	RAATTY	1 cut(s)	768
AcuI	CTGAAG	2 cut(s)	293, 341
AcvI	CACGTG	1 cut(s)	640
AfiI	CCNNNNNNNGG	2 cut(s)	190, 801
AflIII	ACRYGT	1 cut(s)	637
AgeI	ACCGGT	1 cut(s)	71
AgsI	TTSAA	6 cut(s)	305, 439, 590, 758, 782, 1304
AjiI	CACGTC	1 cut(s)	82
AjnI	CCWGG	3 cut(s)	62, 156, 423
AloI	GAACNNNNNNTCC	2 cut(s)	185, 217
Alw26I	GTCTC	1 cut(s)	477
AlwI	GGATC	2 cut(s)	243, 948
AlwNI	CAGNNNCTG	1 cut(s)	364
Ama87I	CYCGRG	1 cut(s)	94
ApeKI	GCWGC	4 cut(s)	364, 497, 718, 1177
ApoI	RAATTY	1 cut(s)	768
AsiGI	ACCGGT	1 cut(s)	71
AspLEI	GCGC	1 cut(s)	310
AspS9I	GGNCC	5 cut(s)	56, 264, 389, 749, 830
AsuHPI	GGTGA	6 cut(s)	606, 665, 859, 1166, 1214, 1231
AsuNHI	GCTAGC	1 cut(s)	964
AvaI	CYCGRG	1 cut(s)	94
AvaII	GGWCC	1 cut(s)	749
BaeGI	GKGCMC	1 cut(s)	796
BanII	GRGCYC	1 cut(s)	549
BarI	GAAGNNNNNNTAC	2 cut(s)	110, 142
BbrPI	CACGTG	1 cut(s)	640
BbvCI	CCTCAGC	1 cut(s)	464
BbvI	GCAGC	4 cut(s)	351, 509, 730, 1164
BccI	CCATC	6 cut(s)	332, 337, 619, 638, 679, 1199
BceAI	ACGGC	1 cut(s)	532
BcgI	CGANNNNNNTGC	2 cut(s)	272, 306
BciT130I	CCWGG	3 cut(s)	64, 158, 425
BcoDI	GTCTC	1 cut(s)	477
BfaI	CTAG	4 cut(s)	338, 458, 953, 965
BglI	GCCNNNNNGGC	1 cut(s)	553
BisI	GCNGC	6 cut(s)	162, 235, 365, 498, 719, 1178
BlsI	GCNGC	6 cut(s)	163, 236, 366, 499, 720, 1179
Bme1390I	CCNGG	3 cut(s)	64, 158, 425
Bme18I	GGWCC	1 cut(s)	749
BmeT110I	CYCGRG	1 cut(s)	94
BmgBI	CACGTC	1 cut(s)	82
BmgT120I	GGNCC	5 cut(s)	56, 264, 389, 749, 830
BmiI	GGNNCC	2 cut(s)	546, 1054
BmrFI	CCNGG	3 cut(s)	64, 158, 425
BmrI	ACTGGG	1 cut(s)	1084
BmsI	GCATC	3 cut(s)	45, 478, 509
BmtI	GCTAGC	1 cut(s)	968
BmuI	ACTGGG	1 cut(s)	1084
BpmI	CTGGAG	2 cut(s)	393, 563
Bpu10I	CCTNAGC	2 cut(s)	464, 520
BpuEI	CTTGAG	1 cut(s)	150
Bsa29I	ATCGAT	1 cut(s)	327
BsaAI	YACGTR	1 cut(s)	640
BsaBI	GATNNNNATC	2 cut(s)	253, 939
BsaI	GGTCTC	1 cut(s)	477
BsaJI	CCNNGG	2 cut(s)	63, 481
BsaWI	WCCGGW	1 cut(s)	71
BsaXI	ACNNNNNCTCC	2 cut(s)	1200, 1230
Bsc4I	CCNNNNNNNGG	2 cut(s)	190, 801
Bse118I	RCCGGY	5 cut(s)	71, 237, 261, 977, 1120
Bse1I	ACTGG	3 cut(s)	12, 410, 1079
Bse8I	GATNNNNATC	2 cut(s)	253, 939
BseBI	CCWGG	3 cut(s)	64, 158, 425
BseCI	ATCGAT	1 cut(s)	327
BseDI	CCNNGG	2 cut(s)	63, 481
BseGI	GGATG	1 cut(s)	348
BseJI	GATNNNNATC	2 cut(s)	253, 939
BseLI	CCNNNNNNNGG	2 cut(s)	190, 801
BseMII	CTCAG	2 cut(s)	478, 1254
BseNI	ACTGG	3 cut(s)	12, 410, 1079
BseRI	GAGGAG	1 cut(s)	150
BseSI	GKGCMC	1 cut(s)	796
BseX3I	CGGCCG	2 cut(s)	235, 259
BseXI	GCAGC	4 cut(s)	351, 509, 730, 1164
BseYI	CCCAGC	2 cut(s)	548, 1184
Bsh1285I	CGRYCG	3 cut(s)	54, 238, 262
BshTI	ACCGGT	1 cut(s)	71
BshVI	ATCGAT	1 cut(s)	327
BsiEI	CGRYCG	3 cut(s)	54, 238, 262
BsiHKCI	CYCGRG	1 cut(s)	94
BsiSI	CCGG	6 cut(s)	72, 238, 258, 262, 978, 1121
BslFI	GGGAC	2 cut(s)	654, 1125
BslI	CCNNNNNNNGG	2 cut(s)	190, 801
BsmAI	GTCTC	1 cut(s)	477
BsmFI	GGGAC	2 cut(s)	654, 1125
Bso31I	GGTCTC	1 cut(s)	477
BsoBI	CYCGRG	1 cut(s)	94
Bsp1286I	GDGCHC	2 cut(s)	549, 796
Bsp143I	GATC	3 cut(s)	248, 940, 1279
BspACI	CCGC	4 cut(s)	161, 184, 234, 288
BspCNI	CTCAG	2 cut(s)	477, 1255
BspDI	ATCGAT	1 cut(s)	327
BspLI	GGNNCC	2 cut(s)	546, 1054
BspOI	GCTAGC	1 cut(s)	968
BspPI	GGATC	2 cut(s)	243, 948
BspTNI	GGTCTC	1 cut(s)	477
BsrFI	RCCGGY	5 cut(s)	71, 237, 261, 977, 1120
BsrI	ACTGG	3 cut(s)	12, 410, 1079
BssAI	RCCGGY	5 cut(s)	71, 237, 261, 977, 1120
BssECI	CCNNGG	2 cut(s)	63, 481
BssMI	GATC	3 cut(s)	248, 940, 1279
Bst2UI	CCWGG	3 cut(s)	64, 158, 425
Bst4CI	ACNGT	5 cut(s)	706, 820, 893, 910, 1225
Bst6I	CTCTTC	4 cut(s)	360, 425, 999, 1207
BstBAI	YACGTR	1 cut(s)	640
BstC8I	GCNNGC	9 cut(s)	60, 263, 369, 554, 966, 975, 979, 1130, 1182
BstDEI	CTNAG	5 cut(s)	7, 131, 464, 520, 1263
BstEII	GGTNACC	1 cut(s)	67
BstF5I	GGATG	1 cut(s)	348
BstHHI	GCGC	1 cut(s)	310
BstKTI	GATC	3 cut(s)	251, 943, 1282
BstMAI	GTCTC	1 cut(s)	477
BstMBI	GATC	3 cut(s)	248, 940, 1279
BstMCI	CGRYCG	3 cut(s)	54, 238, 262
BstMWI	GCNNNNNNNGC	4 cut(s)	497, 553, 970, 974
BstNI	CCWGG	3 cut(s)	64, 158, 425
BstNSI	RCATGY	1 cut(s)	234
BstPI	GGTNACC	1 cut(s)	67
BstSCI	CCNGG	3 cut(s)	62, 156, 423
BstSLI	GKGCMC	1 cut(s)	796
BstV1I	GCAGC	4 cut(s)	351, 509, 730, 1164
BstX2I	RGATCY	2 cut(s)	248, 940
BstXI	CCANNNNNNTGG	1 cut(s)	386
BstYI	RGATCY	2 cut(s)	248, 940
BstZI	CGGCCG	2 cut(s)	235, 259
Bsu15I	ATCGAT	1 cut(s)	327
BsuTUI	ATCGAT	1 cut(s)	327
BtrI	CACGTC	1 cut(s)	82
BtsCI	GGATG	1 cut(s)	348
BtsI	GCAGTG	1 cut(s)	317
BtsIMutI	CAGTG	2 cut(s)	317, 702
Cac8I	GCNNGC	9 cut(s)	60, 263, 369, 554, 966, 975, 979, 1130, 1182
CaiI	CAGNNNCTG	1 cut(s)	364
CfoI	GCGC	1 cut(s)	310
Cfr10I	RCCGGY	5 cut(s)	71, 237, 261, 977, 1120
Cfr13I	GGNCC	5 cut(s)	56, 264, 389, 749, 830
ClaI	ATCGAT	1 cut(s)	327
CspAI	ACCGGT	1 cut(s)	71
CviAII	CATG	5 cut(s)	103, 207, 231, 476, 532
DdeI	CTNAG	5 cut(s)	7, 131, 464, 520, 1263
DpnI	GATC	3 cut(s)	250, 942, 1281
DpnII	GATC	3 cut(s)	248, 940, 1279
EaeI	YGGCCR	3 cut(s)	235, 259, 979
EagI	CGGCCG	2 cut(s)	235, 259
Eam1104I	CTCTTC	4 cut(s)	360, 425, 999, 1207
EarI	CTCTTC	4 cut(s)	360, 425, 999, 1207
EclXI	CGGCCG	2 cut(s)	235, 259
Eco147I	AGGCCT	2 cut(s)	171, 462
Eco24I	GRGCYC	1 cut(s)	549
Eco31I	GGTCTC	1 cut(s)	477
Eco47I	GGWCC	1 cut(s)	749
Eco52I	CGGCCG	2 cut(s)	235, 259
Eco57I	CTGAAG	2 cut(s)	293, 341
Eco72I	CACGTG	1 cut(s)	640
Eco88I	CYCGRG	1 cut(s)	94
Eco91I	GGTNACC	1 cut(s)	67
EcoO65I	GGTNACC	1 cut(s)	67
EcoRII	CCWGG	3 cut(s)	62, 156, 423
EcoT22I	ATGCAT	1 cut(s)	493
EcoT38I	GRGCYC	1 cut(s)	549
FaeI	CATG	5 cut(s)	106, 210, 234, 479, 535
FaqI	GGGAC	2 cut(s)	654, 1125
FatI	CATG	5 cut(s)	102, 206, 230, 475, 531
Fnu4HI	GCNGC	6 cut(s)	162, 235, 365, 498, 719, 1178
FokI	GGATG	1 cut(s)	355
FriOI	GRGCYC	1 cut(s)	549
FseI	GGCCGGCC	1 cut(s)	265
Fsp4HI	GCNGC	6 cut(s)	162, 235, 365, 498, 719, 1178
FspBI	CTAG	4 cut(s)	338, 458, 953, 965
GlaI	GCGC	1 cut(s)	309
GluI	GCNGC	6 cut(s)	162, 235, 365, 498, 719, 1178
GsaI	CCCAGC	2 cut(s)	552, 1188
GsuI	CTGGAG	2 cut(s)	393, 563
HapII	CCGG	6 cut(s)	72, 238, 258, 262, 978, 1121
HhaI	GCGC	1 cut(s)	310
Hin1II	CATG	5 cut(s)	106, 210, 234, 479, 535
Hin6I	GCGC	1 cut(s)	308
HinP1I	GCGC	1 cut(s)	308
HindIII	AAGCTT	2 cut(s)	41, 119
HinfI	GANTC	3 cut(s)	254, 644, 999
HpaII	CCGG	6 cut(s)	72, 238, 258, 262, 978, 1121
HphI	GGTGA	6 cut(s)	606, 665, 859, 1166, 1214, 1231
Hpy166II	GTNNAC	5 cut(s)	142, 204, 643, 702, 1072
Hpy188III	TCNNGA	4 cut(s)	17, 302, 542, 739
Hpy8I	GTNNAC	5 cut(s)	142, 204, 643, 702, 1072
HpyAV	CCTTC	4 cut(s)	821, 878, 1102, 1154
HpyCH4III	ACNGT	5 cut(s)	706, 820, 893, 910, 1225
HpyCH4IV	ACGT	3 cut(s)	81, 639, 1064
HpyCH4V	TGCA	8 cut(s)	491, 497, 569, 602, 681, 733, 858, 1132
HpyF10VI	GCNNNNNNNGC	4 cut(s)	497, 553, 970, 974
HpyF3I	CTNAG	5 cut(s)	7, 131, 464, 520, 1263
HpySE526I	ACGT	3 cut(s)	81, 639, 1064
Hsp92II	CATG	5 cut(s)	106, 210, 234, 479, 535
HspAI	GCGC	1 cut(s)	308
KroI	GCCGGC	2 cut(s)	261, 977
KroNI	GCCGGC	2 cut(s)	263, 979
Kzo9I	GATC	3 cut(s)	248, 940, 1279
LmnI	GCTCC	3 cut(s)	544, 1052, 1163
Lsp1109I	GCAGC	4 cut(s)	351, 509, 730, 1164
LweI	GCATC	3 cut(s)	45, 478, 509
MaeI	CTAG	4 cut(s)	338, 458, 953, 965
MaeII	ACGT	3 cut(s)	81, 639, 1064
MalI	GATC	3 cut(s)	250, 942, 1281
MboI	GATC	3 cut(s)	248, 940, 1279
MboII	GAAGA	6 cut(s)	347, 394, 412, 1016, 1091, 1224
MflI	RGATCY	2 cut(s)	248, 940
MhlI	GDGCHC	2 cut(s)	549, 796
MluCI	AATT	7 cut(s)	562, 753, 768, 774, 798, 842, 852
MlyI	GAGTC	1 cut(s)	638
MmeI	TCCRAC	1 cut(s)	1080
MnlI	CCTC	8 cut(s)	128, 288, 473, 491, 907, 1000, 1202, 1263
Mph1103I	ATGCAT	1 cut(s)	493
MroNI	GCCGGC	2 cut(s)	261, 977
MslI	CAYNNNNRTG	1 cut(s)	211
MspA1I	CMGCKG	1 cut(s)	468
MspI	CCGG	6 cut(s)	72, 238, 258, 262, 978, 1121
MspR9I	CCNGG	3 cut(s)	64, 158, 425
MvaI	CCWGG	3 cut(s)	64, 158, 425
MwoI	GCNNNNNNNGC	4 cut(s)	497, 553, 970, 974
NaeI	GCCGGC	2 cut(s)	263, 979
NdeII	GATC	3 cut(s)	248, 940, 1279
NgoMIV	GCCGGC	2 cut(s)	261, 977
NheI	GCTAGC	1 cut(s)	964
NlaIII	CATG	5 cut(s)	106, 210, 234, 479, 535
NlaIV	GGNNCC	2 cut(s)	546, 1054
NmuCI	GTSAC	5 cut(s)	453, 653, 761, 847, 1219
NsiI	ATGCAT	1 cut(s)	493
NspI	RCATGY	1 cut(s)	234
PceI	AGGCCT	2 cut(s)	171, 462
PdiI	GCCGGC	2 cut(s)	263, 979
PfeI	GAWTC	2 cut(s)	254, 999
PinAI	ACCGGT	1 cut(s)	71
PkrI	GCNGC	6 cut(s)	163, 236, 366, 499, 720, 1179
PleI	GAGTC	1 cut(s)	638
PmaCI	CACGTG	1 cut(s)	640
PmlI	CACGTG	1 cut(s)	640
PpsI	GAGTC	1 cut(s)	638
Ppu21I	YACGTR	1 cut(s)	640
PsiI	TTATAA	1 cut(s)	1137
Psp6I	CCWGG	3 cut(s)	62, 156, 423
PspCI	CACGTG	1 cut(s)	640
PspEI	GGTNACC	1 cut(s)	67
PspFI	CCCAGC	2 cut(s)	548, 1184
PspGI	CCWGG	3 cut(s)	62, 156, 423
PspN4I	GGNNCC	2 cut(s)	546, 1054
PspPI	GGNCC	5 cut(s)	56, 264, 389, 749, 830
PstNI	CAGNNNCTG	1 cut(s)	364
PsuI	RGATCY	2 cut(s)	248, 940
PvuII	CAGCTG	1 cut(s)	468
RigI	GGCCGGCC	1 cut(s)	265
RseI	CAYNNNNRTG	1 cut(s)	211
SatI	GCNGC	6 cut(s)	162, 235, 365, 498, 719, 1178
Sau3AI	GATC	3 cut(s)	248, 940, 1279
Sau96I	GGNCC	5 cut(s)	56, 264, 389, 749, 830
SchI	GAGTC	1 cut(s)	638
ScrFI	CCNGG	3 cut(s)	64, 158, 425
SduI	GDGCHC	2 cut(s)	549, 796
SfaNI	GCATC	3 cut(s)	45, 478, 509
SinI	GGWCC	1 cut(s)	749
SmiMI	CAYNNNNRTG	1 cut(s)	211
SmlI	CTYRAG	1 cut(s)	165
SmoI	CTYRAG	1 cut(s)	165
Sse9I	AATT	7 cut(s)	562, 753, 768, 774, 798, 842, 852
SseBI	AGGCCT	2 cut(s)	171, 462
SsiI	CCGC	4 cut(s)	161, 184, 234, 288
SspMI	CTAG	4 cut(s)	338, 458, 953, 965
StuI	AGGCCT	2 cut(s)	171, 462
StyD4I	CCNGG	3 cut(s)	62, 156, 423
TaaI	ACNGT	5 cut(s)	706, 820, 893, 910, 1225
TaiI	ACGT	3 cut(s)	84, 642, 1067
TaqI	TCGA	4 cut(s)	327, 912, 1002, 1086
TaqII	GACCGA	1 cut(s)	40
TasI	AATT	7 cut(s)	562, 753, 768, 774, 798, 842, 852
TauI	GCSGC	2 cut(s)	164, 237
TfiI	GAWTC	2 cut(s)	254, 999
TscAI	CASTG	2 cut(s)	317, 709
TseFI	GTSAC	5 cut(s)	453, 653, 761, 847, 1219
TseI	GCWGC	4 cut(s)	364, 497, 718, 1177
Tsp45I	GTSAC	5 cut(s)	453, 653, 761, 847, 1219
TspDTI	ATGAA	6 cut(s)	36, 77, 131, 345, 499, 1173
TspRI	CASTG	2 cut(s)	317, 709
VpaK11BI	GGWCC	1 cut(s)	749
XapI	RAATTY	1 cut(s)	768
XceI	RCATGY	1 cut(s)	234
XcmI	CCANNNNNNNNNTGG	1 cut(s)	637
XspI	CTAG	4 cut(s)	338, 458, 953, 965
Zsp2I	ATGCAT	1 cut(s)	493

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.