Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0008938

Physical Location & Seq

Genomic Coordinates Reverse (-)

1836830 .. 1839627

Gene Structure 2798 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1016900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1317 bp

ATGGTCTTAGCCAGTCAAGAAAATTTGGTCTATGGCATTAAGCTTTCATCGGTCGGGCCAGCCAGGGTTACCGGTTCGGACGTGGTTCATAACCCGAGTGGCATGGACTTGGCTATGAAGCTTCACTACCTTAGAGGAGTCTACTTCTTTGATAGCCAGGCGGCCCAAGGCATGACCACTAACCGCATAAAGGAAGCTATGTTCACATGGTTATGTGTCAGCTACCGGACATGCGGCCGGTTTCGGAGATCCGAATCCGGCCGGCCCTATCTGAAGTGTAACGATTGCGGTGTGAGGTATCTTGAAGCGCAGTGTGATAAAACCCTCGATGAATGGCTAGGGTTGGACTGCTCTCTTCAGAAGCTGCTTGCTTCTAACCAAATCATTGGCCCTGAACTATTCTTCTCTCCAGTTGTTCTCTTCCAGGTTACTTATTTCAAATGTGGAGGAATATCACTAGGCCTCAGCTGGTCTCATGTCCTCGGAGATGCATTTGCAGCATCAGATTTCATCAACGGCTTAGGGCAAGTCATGTCTGGTCTGGAGATCAGCAAGCTACCAAATTATGCAAATCCGAACACAAATGTTCAACAAAAACTTGCAAAAAATCCATCACCACCACCACTCTCCATCAGACACGTGGACTCGGTTGGTGACTATTGGATAAGTCCCAATAATTGCAAGATGGAAACATTCTCGTTCCCTGTAACTGCCACGCAGCTAAACAACTTGCAAGTCAAGATATTAGGTCCAATTCAAAGTGACCAAATTCCAATTTTTGAACTTATTTGTGCCCTAATTTGGAAATGCATAGCCACAGTTAGAGAAGGGCCACAACCAAAATTGGTGACAATTTGCAAAAATGATACAAATAGGAGAACAGAAGGTGAATGCAGCAACAGTCAGACTATAAGCACAGTTGAGGCAGATTTTAGGGTTTCGGATATGGATCCAAAAGAACTAGCAAACTTGCTAGCCAAGCAAGCCGGCCAGAACGAGAAAACACGAATCGAAGAGGCCATAGAGAATGAGAATGGAGTAGCTGATTTTGTTGTGTATGGAGCCAACTTGACGTTTGTGAACTGGGAAGATGTTGATTTTTATGGGTTGGAAGTGAAGGGACATAAGCCGGTTTGCGTGCATTATAACATACAAGGTGTTGGTGATGAAGGAGCTGTTTTGTTGCTGCCTGCTGGGGCTAAAGAGTTTGGTGATGGAGGAAGAGTGGTGAGTGTTATTCTGCCAGAGAAAGAAGTGTTTGGGGTCAAATCTGAGTTGAGGAAAAATGATCTGCTGTTTGGTAATGAGCTTGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000038 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006629 GO:0006631 GO:0006633 GO:0007275 GO:0008150 GO:0008152 GO:0008610 GO:0009058 GO:0009507 GO:0009534 GO:0009535 GO:0009536 GO:0009555 GO:0009579 GO:0009987 GO:0010025 GO:0010166 GO:0016020 GO:0016053 GO:0019752 GO:0031976 GO:0031984 GO:0032501 GO:0032502 GO:0032787 GO:0034357 GO:0042651 GO:0042761 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0048229 GO:0048856 GO:0055035 GO:0071704 GO:0072330 GO:1901568 GO:1901570 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Transferase

Protein Analysis

439

Amino Acids

48.35

Weight (kDa)

5.37

Isoelectric Point (pI)

34.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Transferase	PF02458	33 - 425	1e-33	Transferase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0008938)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G23840 AT4G13840
fragaria_vesca	FvH4_4g13360
malus_domestica	MD04G1016700.v1.1 MD04G1016900.v1.1
prunus_persica	Prupe.1G163500_v2.0.a1
pyrus_communis	pycom04g01280 pycom04g01300
rosa_chinensis	RchiOBHm_Chr4g0413701
rosa_laevigata	RLG00000008200
rosa_multiflora	Rmu_co8063286.1_g000001 Rmu_co8298563.1_g000001 Rmu_sc0004133.1_g000007 Rmu_sc0033890.1_g000002
rosa_roxburghii	Rroxscaffold_5G00357640
rosa_rugosa	Rorug04G0122700
rosa_samantha	Rh4AG181900 Rh4BG181000 Rh4BG181100 Rh4CG193100 Rh4DG177500
rosa_wichuraiana	Rw4G015220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1146
AccI	GTMKAC	1 cut(s)	141
AciI	CCGC	4 cut(s)	161, 184, 234, 288
AclWI	GGATC	3 cut(s)	243, 944, 957
AcoI	YGGCCR	3 cut(s)	235, 259, 988
AcsI	RAATTY	2 cut(s)	22, 768
AcuI	CTGAAG	2 cut(s)	293, 341
AcvI	CACGTG	1 cut(s)	640
AfiI	CCNNNNNNNGG	2 cut(s)	190, 801
AflIII	ACRYGT	1 cut(s)	637
AgeI	ACCGGT	1 cut(s)	71
AgsI	TTSAA	6 cut(s)	305, 439, 590, 758, 782, 1313
AjiI	CACGTC	1 cut(s)	82
AjnI	CCWGG	3 cut(s)	62, 156, 423
AloI	GAACNNNNNNTCC	2 cut(s)	185, 217
Alw26I	GTCTC	1 cut(s)	477
AlwI	GGATC	3 cut(s)	243, 944, 957
AlwNI	CAGNNNCTG	1 cut(s)	364
Ama87I	CYCGRG	1 cut(s)	94
ApeKI	GCWGC	5 cut(s)	364, 497, 718, 894, 1186
ApoI	RAATTY	2 cut(s)	22, 768
AsiGI	ACCGGT	1 cut(s)	71
AspLEI	GCGC	1 cut(s)	310
AspS9I	GGNCC	6 cut(s)	56, 163, 264, 389, 749, 830
AsuHPI	GGTGA	7 cut(s)	606, 665, 859, 899, 1175, 1223, 1240
AsuNHI	GCTAGC	1 cut(s)	973
AvaI	CYCGRG	1 cut(s)	94
AvaII	GGWCC	1 cut(s)	749
BaeGI	GKGCMC	1 cut(s)	796
BamHI	GGATCC	1 cut(s)	949
BarI	GAAGNNNNNNTAC	2 cut(s)	110, 142
BbrPI	CACGTG	1 cut(s)	640
BbvCI	CCTCAGC	1 cut(s)	464
BbvI	GCAGC	5 cut(s)	351, 509, 730, 906, 1173
BccI	CCATC	4 cut(s)	619, 638, 679, 1208
BceAI	ACGGC	1 cut(s)	532
BciT130I	CCWGG	3 cut(s)	64, 158, 425
BcoDI	GTCTC	1 cut(s)	477
BfaI	CTAG	4 cut(s)	338, 458, 962, 974
BisI	GCNGC	7 cut(s)	162, 235, 365, 498, 719, 895, 1187
BlsI	GCNGC	7 cut(s)	163, 236, 366, 499, 720, 896, 1188
Bme1390I	CCNGG	3 cut(s)	64, 158, 425
Bme18I	GGWCC	1 cut(s)	749
BmeT110I	CYCGRG	1 cut(s)	94
BmgBI	CACGTC	1 cut(s)	82
BmgT120I	GGNCC	6 cut(s)	56, 163, 264, 389, 749, 830
BmiI	GGNNCC	2 cut(s)	951, 1063
BmrFI	CCNGG	3 cut(s)	64, 158, 425
BmrI	ACTGGG	1 cut(s)	1093
BmsI	GCATC	2 cut(s)	478, 509
BmtI	GCTAGC	1 cut(s)	977
BmuI	ACTGGG	1 cut(s)	1093
BpmI	CTGGAG	2 cut(s)	393, 563
Bpu10I	CCTNAGC	2 cut(s)	464, 520
BsaAI	YACGTR	1 cut(s)	640
BsaBI	GATNNNNATC	2 cut(s)	253, 948
BsaI	GGTCTC	1 cut(s)	477
BsaJI	CCNNGG	3 cut(s)	63, 166, 481
BsaWI	WCCGGW	2 cut(s)	71, 225
BsaXI	ACNNNNNCTCC	2 cut(s)	1209, 1239
Bsc4I	CCNNNNNNNGG	2 cut(s)	190, 801
Bse118I	RCCGGY	5 cut(s)	71, 237, 261, 986, 1129
Bse1I	ACTGG	3 cut(s)	12, 410, 1088
Bse8I	GATNNNNATC	2 cut(s)	253, 948
BseBI	CCWGG	3 cut(s)	64, 158, 425
BseDI	CCNNGG	3 cut(s)	63, 166, 481
BseJI	GATNNNNATC	2 cut(s)	253, 948
BseLI	CCNNNNNNNGG	2 cut(s)	190, 801
BseMII	CTCAG	2 cut(s)	478, 1263
BseNI	ACTGG	3 cut(s)	12, 410, 1088
BseRI	GAGGAG	1 cut(s)	150
BseSI	GKGCMC	1 cut(s)	796
BseX3I	CGGCCG	2 cut(s)	235, 259
BseXI	GCAGC	5 cut(s)	351, 509, 730, 906, 1173
BseYI	CCCAGC	1 cut(s)	1193
Bsh1285I	CGRYCG	3 cut(s)	54, 238, 262
BshTI	ACCGGT	1 cut(s)	71
BsiEI	CGRYCG	3 cut(s)	54, 238, 262
BsiHKCI	CYCGRG	1 cut(s)	94
BsiSI	CCGG	7 cut(s)	72, 226, 238, 258, 262, 987, 1130
BslFI	GGGAC	2 cut(s)	654, 1134
BslI	CCNNNNNNNGG	2 cut(s)	190, 801
BsmAI	GTCTC	1 cut(s)	477
BsmFI	GGGAC	2 cut(s)	654, 1134
BsmI	GAATGC	1 cut(s)	896
Bso31I	GGTCTC	1 cut(s)	477
BsoBI	CYCGRG	1 cut(s)	94
Bsp1286I	GDGCHC	1 cut(s)	796
Bsp143I	GATC	4 cut(s)	248, 546, 949, 1288
BspACI	CCGC	4 cut(s)	161, 184, 234, 288
BspCNI	CTCAG	2 cut(s)	477, 1264
BspLI	GGNNCC	2 cut(s)	951, 1063
BspOI	GCTAGC	1 cut(s)	977
BspPI	GGATC	3 cut(s)	243, 944, 957
BspTNI	GGTCTC	1 cut(s)	477
BsrFI	RCCGGY	5 cut(s)	71, 237, 261, 986, 1129
BsrI	ACTGG	3 cut(s)	12, 410, 1088
BssAI	RCCGGY	5 cut(s)	71, 237, 261, 986, 1129
BssECI	CCNNGG	3 cut(s)	63, 166, 481
BssMI	GATC	4 cut(s)	248, 546, 949, 1288
BssT1I	CCWWGG	1 cut(s)	166
Bst2UI	CCWGG	3 cut(s)	64, 158, 425
Bst4CI	ACNGT	3 cut(s)	820, 902, 919
Bst6I	CTCTTC	4 cut(s)	360, 425, 1008, 1216
BstBAI	YACGTR	1 cut(s)	640
BstC8I	GCNNGC	9 cut(s)	60, 263, 369, 554, 975, 984, 988, 1139, 1191
BstDEI	CTNAG	5 cut(s)	7, 131, 464, 520, 1272
BstEII	GGTNACC	1 cut(s)	67
BstHHI	GCGC	1 cut(s)	310
BstKTI	GATC	4 cut(s)	251, 549, 952, 1291
BstMAI	GTCTC	1 cut(s)	477
BstMBI	GATC	4 cut(s)	248, 546, 949, 1288
BstMCI	CGRYCG	3 cut(s)	54, 238, 262
BstMWI	GCNNNNNNNGC	3 cut(s)	497, 979, 983
BstNI	CCWGG	3 cut(s)	64, 158, 425
BstNSI	RCATGY	1 cut(s)	234
BstPI	GGTNACC	1 cut(s)	67
BstSCI	CCNGG	3 cut(s)	62, 156, 423
BstSLI	GKGCMC	1 cut(s)	796
BstV1I	GCAGC	5 cut(s)	351, 509, 730, 906, 1173
BstX2I	RGATCY	2 cut(s)	248, 949
BstXI	CCANNNNNNTGG	1 cut(s)	386
BstYI	RGATCY	2 cut(s)	248, 949
BstZI	CGGCCG	2 cut(s)	235, 259
BtrI	CACGTC	1 cut(s)	82
BtsI	GCAGTG	1 cut(s)	317
BtsIMutI	CAGTG	1 cut(s)	317
Cac8I	GCNNGC	9 cut(s)	60, 263, 369, 554, 975, 984, 988, 1139, 1191
CaiI	CAGNNNCTG	1 cut(s)	364
CfoI	GCGC	1 cut(s)	310
Cfr10I	RCCGGY	5 cut(s)	71, 237, 261, 986, 1129
Cfr13I	GGNCC	6 cut(s)	56, 163, 264, 389, 749, 830
CspAI	ACCGGT	1 cut(s)	71
CviAII	CATG	6 cut(s)	103, 172, 207, 231, 476, 532
DdeI	CTNAG	5 cut(s)	7, 131, 464, 520, 1272
DpnI	GATC	4 cut(s)	250, 548, 951, 1290
DpnII	GATC	4 cut(s)	248, 546, 949, 1288
EaeI	YGGCCR	3 cut(s)	235, 259, 988
EagI	CGGCCG	2 cut(s)	235, 259
Eam1104I	CTCTTC	4 cut(s)	360, 425, 1008, 1216
EarI	CTCTTC	4 cut(s)	360, 425, 1008, 1216
EclXI	CGGCCG	2 cut(s)	235, 259
Eco130I	CCWWGG	1 cut(s)	166
Eco147I	AGGCCT	1 cut(s)	462
Eco31I	GGTCTC	1 cut(s)	477
Eco47I	GGWCC	1 cut(s)	749
Eco52I	CGGCCG	2 cut(s)	235, 259
Eco57I	CTGAAG	2 cut(s)	293, 341
Eco72I	CACGTG	1 cut(s)	640
Eco88I	CYCGRG	1 cut(s)	94
Eco91I	GGTNACC	1 cut(s)	67
EcoO65I	GGTNACC	1 cut(s)	67
EcoRII	CCWGG	3 cut(s)	62, 156, 423
EcoT14I	CCWWGG	1 cut(s)	166
EcoT22I	ATGCAT	2 cut(s)	493, 812
ErhI	CCWWGG	1 cut(s)	166
FaeI	CATG	6 cut(s)	106, 175, 210, 234, 479, 535
FaqI	GGGAC	2 cut(s)	654, 1134
FatI	CATG	6 cut(s)	102, 171, 206, 230, 475, 531
FblI	GTMKAC	1 cut(s)	141
Fnu4HI	GCNGC	7 cut(s)	162, 235, 365, 498, 719, 895, 1187
FseI	GGCCGGCC	1 cut(s)	265
Fsp4HI	GCNGC	7 cut(s)	162, 235, 365, 498, 719, 895, 1187
FspBI	CTAG	4 cut(s)	338, 458, 962, 974
GlaI	GCGC	1 cut(s)	309
GluI	GCNGC	7 cut(s)	162, 235, 365, 498, 719, 895, 1187
GsaI	CCCAGC	1 cut(s)	1197
GsuI	CTGGAG	2 cut(s)	393, 563
HapII	CCGG	7 cut(s)	72, 226, 238, 258, 262, 987, 1130
HhaI	GCGC	1 cut(s)	310
Hin1II	CATG	6 cut(s)	106, 175, 210, 234, 479, 535
Hin6I	GCGC	1 cut(s)	308
HinP1I	GCGC	1 cut(s)	308
HindIII	AAGCTT	2 cut(s)	41, 119
HinfI	GANTC	4 cut(s)	138, 254, 644, 1008
HpaII	CCGG	7 cut(s)	72, 226, 238, 258, 262, 987, 1130
HphI	GGTGA	7 cut(s)	606, 665, 859, 899, 1175, 1223, 1240
Hpy166II	GTNNAC	4 cut(s)	142, 204, 643, 1081
Hpy188III	TCNNGA	4 cut(s)	17, 302, 542, 739
Hpy8I	GTNNAC	4 cut(s)	142, 204, 643, 1081
HpyAV	CCTTC	4 cut(s)	821, 878, 1111, 1163
HpyCH4III	ACNGT	3 cut(s)	820, 902, 919
HpyCH4IV	ACGT	3 cut(s)	81, 639, 1073
HpyF10VI	GCNNNNNNNGC	3 cut(s)	497, 979, 983
HpyF3I	CTNAG	5 cut(s)	7, 131, 464, 520, 1272
HpySE526I	ACGT	3 cut(s)	81, 639, 1073
Hsp92II	CATG	6 cut(s)	106, 175, 210, 234, 479, 535
HspAI	GCGC	1 cut(s)	308
KroI	GCCGGC	2 cut(s)	261, 986
KroNI	GCCGGC	2 cut(s)	263, 988
Kzo9I	GATC	4 cut(s)	248, 546, 949, 1288
LmnI	GCTCC	2 cut(s)	1061, 1172
Lsp1109I	GCAGC	5 cut(s)	351, 509, 730, 906, 1173
LweI	GCATC	2 cut(s)	478, 509
MaeI	CTAG	4 cut(s)	338, 458, 962, 974
MaeII	ACGT	3 cut(s)	81, 639, 1073
MaeIII	GTNAC	7 cut(s)	67, 278, 427, 653, 706, 761, 847
MalI	GATC	4 cut(s)	250, 548, 951, 1290
MboI	GATC	4 cut(s)	248, 546, 949, 1288
MboII	GAAGA	6 cut(s)	347, 394, 412, 1025, 1100, 1233
MflI	RGATCY	2 cut(s)	248, 949
MhlI	GDGCHC	1 cut(s)	796
MluCI	AATT	9 cut(s)	22, 562, 676, 753, 768, 774, 798, 842, 852
MlyI	GAGTC	2 cut(s)	147, 638
MmeI	TCCRAC	2 cut(s)	324, 1089
Mph1103I	ATGCAT	2 cut(s)	493, 812
MroNI	GCCGGC	2 cut(s)	261, 986
MseI	TTAA	1 cut(s)	39
MslI	CAYNNNNRTG	1 cut(s)	211
MspA1I	CMGCKG	1 cut(s)	468
MspI	CCGG	7 cut(s)	72, 226, 238, 258, 262, 987, 1130
MspR9I	CCNGG	3 cut(s)	64, 158, 425
Mva1269I	GAATGC	1 cut(s)	896
MvaI	CCWGG	3 cut(s)	64, 158, 425
MwoI	GCNNNNNNNGC	3 cut(s)	497, 979, 983
NaeI	GCCGGC	2 cut(s)	263, 988
NdeII	GATC	4 cut(s)	248, 546, 949, 1288
NgoMIV	GCCGGC	2 cut(s)	261, 986
NheI	GCTAGC	1 cut(s)	973
NlaIII	CATG	6 cut(s)	106, 175, 210, 234, 479, 535
NlaIV	GGNNCC	2 cut(s)	951, 1063
NmuCI	GTSAC	3 cut(s)	653, 761, 847
NsiI	ATGCAT	2 cut(s)	493, 812
NspI	RCATGY	1 cut(s)	234
PceI	AGGCCT	1 cut(s)	462
PctI	GAATGC	1 cut(s)	896
PdiI	GCCGGC	2 cut(s)	263, 988
PfeI	GAWTC	2 cut(s)	254, 1008
PinAI	ACCGGT	1 cut(s)	71
PkrI	GCNGC	7 cut(s)	163, 236, 366, 499, 720, 896, 1188
PleI	GAGTC	2 cut(s)	146, 638
PmaCI	CACGTG	1 cut(s)	640
PmlI	CACGTG	1 cut(s)	640
PpsI	GAGTC	2 cut(s)	146, 638
Ppu21I	YACGTR	1 cut(s)	640
PsiI	TTATAA	1 cut(s)	1146
Psp6I	CCWGG	3 cut(s)	62, 156, 423
PspCI	CACGTG	1 cut(s)	640
PspEI	GGTNACC	1 cut(s)	67
PspFI	CCCAGC	1 cut(s)	1193
PspGI	CCWGG	3 cut(s)	62, 156, 423
PspN4I	GGNNCC	2 cut(s)	951, 1063
PspPI	GGNCC	6 cut(s)	56, 163, 264, 389, 749, 830
PstNI	CAGNNNCTG	1 cut(s)	364
PsuI	RGATCY	2 cut(s)	248, 949
PvuII	CAGCTG	1 cut(s)	468
RigI	GGCCGGCC	1 cut(s)	265
RseI	CAYNNNNRTG	1 cut(s)	211
SaqAI	TTAA	1 cut(s)	39
SatI	GCNGC	7 cut(s)	162, 235, 365, 498, 719, 895, 1187
Sau3AI	GATC	4 cut(s)	248, 546, 949, 1288
Sau96I	GGNCC	6 cut(s)	56, 163, 264, 389, 749, 830
SchI	GAGTC	2 cut(s)	147, 638
ScrFI	CCNGG	3 cut(s)	64, 158, 425
SduI	GDGCHC	1 cut(s)	796
SfaNI	GCATC	2 cut(s)	478, 509
SinI	GGWCC	1 cut(s)	749
SmiMI	CAYNNNNRTG	1 cut(s)	211
Sse9I	AATT	9 cut(s)	22, 562, 676, 753, 768, 774, 798, 842, 852
SseBI	AGGCCT	1 cut(s)	462
SsiI	CCGC	4 cut(s)	161, 184, 234, 288
SspMI	CTAG	4 cut(s)	338, 458, 962, 974
StuI	AGGCCT	1 cut(s)	462
StyD4I	CCNGG	3 cut(s)	62, 156, 423
StyI	CCWWGG	1 cut(s)	166
TaaI	ACNGT	3 cut(s)	820, 902, 919
TaiI	ACGT	3 cut(s)	84, 642, 1076
TaqI	TCGA	2 cut(s)	327, 1011
TaqII	GACCGA	1 cut(s)	40
TasI	AATT	9 cut(s)	22, 562, 676, 753, 768, 774, 798, 842, 852
TauI	GCSGC	2 cut(s)	164, 237
TfiI	GAWTC	2 cut(s)	254, 1008
Tru1I	TTAA	1 cut(s)	39
Tru9I	TTAA	1 cut(s)	39
TscAI	CASTG	1 cut(s)	317
TseFI	GTSAC	3 cut(s)	653, 761, 847
TseI	GCWGC	5 cut(s)	364, 497, 718, 894, 1186
Tsp45I	GTSAC	3 cut(s)	653, 761, 847
TspDTI	ATGAA	6 cut(s)	36, 77, 131, 345, 499, 1182
TspRI	CASTG	1 cut(s)	317
VpaK11BI	GGWCC	1 cut(s)	749
XapI	RAATTY	2 cut(s)	22, 768
XceI	RCATGY	1 cut(s)	234
XcmI	CCANNNNNNNNNTGG	1 cut(s)	637
XmiI	GTMKAC	1 cut(s)	141
XspI	CTAG	4 cut(s)	338, 458, 962, 974
Zsp2I	ATGCAT	2 cut(s)	493, 812

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.